Homology
BLAST of Lag0025721 vs. NCBI nr
Match:
XP_022142619.1 (subtilisin-like protease SBT5.4 [Momordica charantia])
HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 588/762 (77.17%), Postives = 651/762 (85.43%), Query Frame = 0
Query: 102 AYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHIN 161
++ KK YVVLLGSHSHGLE+SE DLQRV NSHY+LLGS LGS KKAKDAIFYSYKKHIN
Sbjct: 25 SFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYSYKKHIN 84
Query: 162 GFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMA 221
GFAA+LEEE+AA LAKH EVAVVL +EMM+LHTTHS G + +P +
Sbjct: 85 GFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSS-----IPSSAWG 144
Query: 222 LHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKF 281
FGE+ +NLDSGVWPESMSF+D GI KPIPS+WKGSCE GF CNRKLIGAKF
Sbjct: 145 KARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCE----QGFPCNRKLIGAKF 204
Query: 282 FNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKG 341
+NEGY+AYMR++N NF+VS+V NS D AGHGSHTLSTAGGNFVA K+VFGSSGFG AKG
Sbjct: 205 YNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKG 264
Query: 342 GSPRARVAAYKVCWGDNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIAS 401
GSP+AR+AAYKVCWG NVGCP ADILQG+D+AIHDGVDV+SYS+GG P+YQD FAIAS
Sbjct: 265 GSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVIVPYYQDFFAIAS 324
Query: 402 FHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGESL 461
F AVKNGISVVCSAGN+GP PG+AVNLAPW+ TVGASTLDRRI + VQLNNGQ FQG SL
Sbjct: 325 FQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSL 384
Query: 462 SKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARIDKGI 521
SKGLPEEKYYPLISGGQAAA GAY DAMLCK G LDPAKVKGKILAC+RGETARIDKG
Sbjct: 385 SKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGT 444
Query: 522 QAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIVPPT 581
+A AGAVGM+LCND+ +GE +D HVLPA IGY+ LAVF+Y+DNTQNP AY+VPP+
Sbjct: 445 EAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPS 504
Query: 582 EKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPY 641
EKFD+ PAP MA FSSRGPN+ITPEIIKPDITAPGVNIIAA TQ+H PS+DPS R+ PY
Sbjct: 505 EKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPY 564
Query: 642 VSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGEL 701
++MSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMT+ARVRDNTM PM DGGS RG+
Sbjct: 565 MTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDP 624
Query: 702 ATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEA-YQCPPSAS 761
ATPFSYGSGHVQPV AMDPGLVYDLTTEDYL+FLCALGYD SM+R FSD+ Y CPPSAS
Sbjct: 625 ATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSAS 684
Query: 762 LLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQVGE 821
LL+FNYPSI VQNL NG+VTVTRKLKNVGSPG Y+ASVRRPEGV V VEPRVL+F+ VGE
Sbjct: 685 LLDFNYPSIGVQNL-NGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGE 744
Query: 822 EKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
EKSF+VTL+G P NAVVDG LIWSDGKHFV SPIVVSSG F
Sbjct: 745 EKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 776
BLAST of Lag0025721 vs. NCBI nr
Match:
XP_022142562.1 (subtilisin-like protease SBT5.4 [Momordica charantia])
HSP 1 Score: 1000.0 bits (2584), Expect = 1.3e-287
Identity = 500/766 (65.27%), Postives = 607/766 (79.24%), Query Frame = 0
Query: 99 INNAYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKK 158
I+ A KKSYVV+LGSHSHGLEVSEADLQRV +SH+KLLGSFLGS++KAKDAIFYSYKK
Sbjct: 21 ISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKK 80
Query: 159 HINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYT 218
+INGFAA+LEEEEAAELAKH EVA VL N+ +LHTTHS + G +VP T
Sbjct: 81 NINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNG-----VVPPT 140
Query: 219 FMALHA-FGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLI 278
A FG++ +NLD+GVWPES SF ++GI +P+KWKG C + + CNRKLI
Sbjct: 141 SPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRKLI 200
Query: 279 GAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFG 338
GAK+FN+G+IAY++++N++ D+SS+ NST DY GHGSHTLSTAGG+FV VFG SG G
Sbjct: 201 GAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFG-SGLG 260
Query: 339 SAKGGSPRARVAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDG 398
+AKGGSP+ARVAAYK+CW + GC ADI +G D AIHDGV+VIS SVGG P+ ++ D
Sbjct: 261 TAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDDS 320
Query: 399 FAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSF 458
AIA+FHAVK GI VVCSAGNSGPD +A N APW+LTVGASTLDR+ + V+L NGQ F
Sbjct: 321 IAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQRF 380
Query: 459 QGESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETAR 518
QG SLS LPE+K YPLI+G QA A A + DAMLCKP TLD +K KGKILACLRG+ AR
Sbjct: 381 QGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNAR 440
Query: 519 IDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAY 578
+DKG QA AGA GM+LCNDE +G + +AD H+LPA + Y DG AV YI +TQNP Y
Sbjct: 441 VDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMGY 500
Query: 579 IVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHN 638
++PP K + PAP+MA FSSRGPN++TPEIIKPD+TAPGVN+IAA T+A SP+ +P N
Sbjct: 501 LIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFDN 560
Query: 639 RKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGS 698
R P+++MSGTSMSCPH++G+ GLL++LHP+WSP+AIKSAIMTSAR+RDNTM PM DGG+
Sbjct: 561 RTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGT 620
Query: 699 ERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCP 758
+ ATPFSYGSGH++P+GA+DPGLVYDLT +DYL+FLCA+GYDE +R FSD Y+CP
Sbjct: 621 DELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCP 680
Query: 759 PSASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFD 818
PSASLLNFNYPSI VQN+ G VTVTR+LKNVG+PG Y+A VR+PEGV V VEPR LKFD
Sbjct: 681 PSASLLNFNYPSIGVQNM-TGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFD 740
Query: 819 QVGEEKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
+VGEEKSF +T++G P VVDG LIW+DG+HFV SPIV+SSG F
Sbjct: 741 KVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779
BLAST of Lag0025721 vs. NCBI nr
Match:
XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])
HSP 1 Score: 947.2 bits (2447), Expect = 9.9e-272
Identity = 478/762 (62.73%), Postives = 590/762 (77.43%), Query Frame = 0
Query: 99 INNAYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKK 158
I++A KKSYVVLLGSHSHG+E++E DL+RV +SH+KLLGSFLGS +KAKDAIFYSYKK
Sbjct: 17 ISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKK 76
Query: 159 HINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYT 218
HINGFAA L+EEEA +LA+H EVA +L N LHTTHS W + + V+ +
Sbjct: 77 HINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHS---WEFMHL--EKNGVIPSSS 136
Query: 219 FMALHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIG 278
+ G++ +NLD+GVWPES SF + GI P+PSKWKG C + + CNRKLIG
Sbjct: 137 PWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIG 196
Query: 279 AKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGS 338
AK+FN+G+IAY++S N+ + SSVINST DY GHGSHTLSTAGG++V+ VFG SG G+
Sbjct: 197 AKYFNKGFIAYLKSENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFG-SGAGT 256
Query: 339 AKGGSPRARVAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGF 398
AKGGSP+ARVAAYKVCW + GC +DI++ D AIHDGVDV+S S+G P+ + +D
Sbjct: 257 AKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSI 316
Query: 399 AIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQ 458
AIA+FHAVK GI VVCSAGNSGPD +A N APW+LTVGASTLDR + V+L NG +
Sbjct: 317 AIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYM 376
Query: 459 GESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARI 518
G SLSK L K YPLI+G QA A A DAMLCKP TLD +KVKGKILACLRG+ AR+
Sbjct: 377 GSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARV 436
Query: 519 DKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYI 578
DKG QA AGAVGM+LCNDE +G + +AD HVLPA I Y DG AV +YI++T+NP Y+
Sbjct: 437 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYL 496
Query: 579 VPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNR 638
+PP+ K + PAP MA FSSRGPN+I+PEIIKPD+TAPGVN+IAA ++A SP+ + NR
Sbjct: 497 IPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNR 556
Query: 639 KTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSE 698
P+++MSGTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMTSAR+RDNTM PM DGGS
Sbjct: 557 TVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSP 616
Query: 699 RGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCPP 758
ATPF+YGSGH++P GA+DPGLVYDL+ DYL+FLCA GYD+ +R FSD ++CPP
Sbjct: 617 DLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPP 676
Query: 759 SASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQ 818
+AS+LNFNYPSI VQNL NG VTVTRKLKNV +PG Y+A + P+GV V V+P+VLKF++
Sbjct: 677 NASILNFNYPSIGVQNL-NGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFER 736
Query: 819 VGEEKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSS 859
V EEKSF +T++G P + VVDG+LIW+DGKHFV SPIV+SS
Sbjct: 737 VAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISS 770
BLAST of Lag0025721 vs. NCBI nr
Match:
TYK15479.1 (subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa])
HSP 1 Score: 933.7 bits (2412), Expect = 1.1e-267
Identity = 473/756 (62.57%), Postives = 576/756 (76.19%), Query Frame = 0
Query: 108 SYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHINGFAAML 167
SYVVLLGSHSHGLEV+E D + V +SH+KLLGSFL S++KAKD+IFYSYKKHINGFAA L
Sbjct: 755 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814
Query: 168 EEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMALHAFGE 227
++E+A LA H EVA VL N+ +L+TTHS W + + V+ P + + FG+
Sbjct: 815 DDEDATRLANHPEVAAVLPNKPKDLYTTHS---WEFMHL--EKNGVVPPSSPWRMAKFGK 874
Query: 228 E-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKFFNEGYI 287
+ +NLD+GVWPES SF + GI+ P PSKWKG C + +G CN+KLIGAK+FN+GY+
Sbjct: 875 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYL 934
Query: 288 AYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKGGSPRAR 347
Y++S N+ D+SS+INST DY GHGSHTLSTA GN+V VFG SG G+AKGGSP+AR
Sbjct: 935 EYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFG-SGIGTAKGGSPKAR 994
Query: 348 VAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIASFHAVK 407
VAAYKVCW + GC ADI + D AIHDGVDV+S S+GG P + +D AIASFHAVK
Sbjct: 995 VAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVK 1054
Query: 408 NGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGESLSKGLP 467
GI VVC+ GNSGP P +A N APW+LTVGASTLDR + V L NG F G S SKGL
Sbjct: 1055 KGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLT 1114
Query: 468 EEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARIDKGIQAENA 527
K YPLI+G QA A A DAMLCKP TLD +KVKGKIL CLRGETAR+DKG QA A
Sbjct: 1115 GRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 1174
Query: 528 GAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIVPPTEKFDV 587
GAVGM+LCND+ +G I+ DFH+LPA I Y+DG + +YI++ +NP Y++PP K +
Sbjct: 1175 GAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNT 1234
Query: 588 TPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPYVSMSG 647
PAP MA FSSRGPN I+PEIIKPD+TAPGVNIIAA ++A SP+ D S NR TP+++MSG
Sbjct: 1235 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSG 1294
Query: 648 TSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGELATPFS 707
TSMSCPH++G+VGLL+ LHPDWSP+AIKSAIMTS++VRDNT++PM DGGS ATPF+
Sbjct: 1295 TSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFA 1354
Query: 708 YGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCPPSASLLNFNY 767
YGSGH+ P GA+DPGLVYDL+ DYL+FLCA GYDE +R FSDE ++CPP++S+LN NY
Sbjct: 1355 YGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNY 1414
Query: 768 PSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQVGEEKSFTV 827
PSI VQNL G V+VTRKLKNVGSPG Y+A + P GV V V+PR LKF++VGEEKSF +
Sbjct: 1415 PSIGVQNL-KGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFEL 1474
Query: 828 TLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
TL+G P + V G LIWSDG+H V SPIVVSSG F
Sbjct: 1475 TLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503
BLAST of Lag0025721 vs. NCBI nr
Match:
KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])
HSP 1 Score: 929.9 bits (2402), Expect = 1.6e-266
Identity = 473/762 (62.07%), Postives = 583/762 (76.51%), Query Frame = 0
Query: 102 AYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHIN 161
A K SY+VLLGSHSHGLEV++ DL+RV +SH+KLLGS GS++KA++AIFYSYKK+IN
Sbjct: 2 AAKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNIN 61
Query: 162 GFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMA 221
GFAA+++EEEAA+LAKH EVA VL N +LHTTHS W + + V+ P +
Sbjct: 62 GFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHS---WEFMHL--EKNGVIPPSSAWR 121
Query: 222 LHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKF 281
G++ +NLD+GVWPES SF + GI P+PSKWKG C + + CNRKLIGAK+
Sbjct: 122 RAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKY 181
Query: 282 FNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKG 341
FN+G++AY++S N + VINST DY GHGSHTLSTAGG++V+ VFG G G+AKG
Sbjct: 182 FNKGFLAYLKSENL---TALVINSTRDYDGHGSHTLSTAGGSYVSGASVFG-LGVGTAKG 241
Query: 342 GSPRARVAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIA 401
GSP+ARVAAYKVCW ++ GC ADI Q D AIHD VDV+S S+GG P+ +Y DG AI+
Sbjct: 242 GSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIS 301
Query: 402 SFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGES 461
+FHAVK GI VVCSAGNSGP + N APW+LTVGAST+DR + V+L NG + G S
Sbjct: 302 AFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSS 361
Query: 462 LSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARIDKG 521
LSKGL +K YPLI+G +A A A + +A LCKP TLD +KVKGKIL CLRG+TAR+DKG
Sbjct: 362 LSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKG 421
Query: 522 IQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIVPP 581
QA AGAVGM+LCNDE +G + +AD HVLPA I Y DG AVF+YI T+NP Y++PP
Sbjct: 422 EQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPP 481
Query: 582 TEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTP 641
T K + PAP MA FSSRGPNLI+PEIIKPD+TAPGVNIIAA ++A SP+ +P NR P
Sbjct: 482 TAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVP 541
Query: 642 YVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGE 701
+++MSGTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMTSAR+RDNT PM DGGS
Sbjct: 542 FITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLA 601
Query: 702 LATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCPPSAS 761
+TPF+YGSGH++P GA+DPGLVYDL+ DYL+FLCA GY+E ++ FSD ++CP SAS
Sbjct: 602 PSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASAS 661
Query: 762 LLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQVGE 821
+LN NYPSI VQNL G VTVTRKLKNV +PG YK VR P GV V+V+P+VLKF++VGE
Sbjct: 662 ILNLNYPSIGVQNL-TGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGE 721
Query: 822 EKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
EKSF +T++G P + VVDG+LIW+DGKHFV SPIVVSS F
Sbjct: 722 EKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753
BLAST of Lag0025721 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 805.4 bits (2079), Expect = 6.1e-232
Identity = 433/764 (56.68%), Postives = 536/764 (70.16%), Query Frame = 0
Query: 102 AYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHIN 161
A+ KKSY+V LGSH+H ++S A L V +SH L SF+GS++ AK+AIFYSYK+HIN
Sbjct: 35 AFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHIN 94
Query: 162 GFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMA 221
GFAA+L+E EAAE+AKH +V V N+ +LHTTHS W + V+ +
Sbjct: 95 GFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHS---WNFMLLAKN--GVVHKSSLWN 154
Query: 222 LHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKF 281
+GE+ +NLD+GVWPES SF D+G +P++WKG C + CNRKLIGA++
Sbjct: 155 KAGYGEDTIIANLDTGVWPESKSFSDEGYG-AVPARWKGRC----HKDVPCNRKLIGARY 214
Query: 282 FNEGYIAYM-RSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAK 341
FN+GY+AY NA+++ + D+ GHGSHTLSTA GNFV +VFG G G+A
Sbjct: 215 FNKGYLAYTGLPSNASYE------TCRDHDGHGSHTLSTAAGNFVPGANVFG-IGNGTAS 274
Query: 342 GGSPRARVAAYKVCWG--DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFA 401
GGSP+ARVAAYKVCW D C ADIL ++ AI DGVDV+S SVGG + DG A
Sbjct: 275 GGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIA 334
Query: 402 IASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQG 461
I SFHAVKNG++VVCSAGNSGP G+ N+APW++TVGAS++DR + V+L NGQSF+G
Sbjct: 335 IGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 394
Query: 462 ESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARID 521
SLSK LPEEK Y LIS A A DA+LCK G+LDP KVKGKIL CLRG+ AR+D
Sbjct: 395 TSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVD 454
Query: 522 KGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIV 581
KG+QA AGA GMVLCND+ +G +I++D HVLPA QI Y+DG +F+Y+ +T++P YI
Sbjct: 455 KGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIK 514
Query: 582 PPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRK 641
PT + PAP MA FSSRGPN ITP I+KPDITAPGVNIIAA T+A P+D S NR+
Sbjct: 515 APTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRR 574
Query: 642 TPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSER 701
TP+ + SGTSMSCPHISGVVGLLKTLHP WSPAAI+SAIMT++R R+N PM D E
Sbjct: 575 TPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVD---ES 634
Query: 702 GELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFS-DEAYQCPP 761
+ A PFSYGSGHVQP A PGLVYDLTT DYLDFLCA+GY+ +++++F+ D Y C
Sbjct: 635 FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ 694
Query: 762 SASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQ 821
A+LL+FNYPSI V NL G +TVTRKLKNVG P Y A R P GV V VEP+ L F++
Sbjct: 695 GANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 754
Query: 822 VGEEKSFTVTLSGASPL----NAVVDGILIWSDGKHFVSSPIVV 857
GE K F +TL PL + V G L W+D H+V SPIVV
Sbjct: 755 TGEVKIFQMTL---RPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774
BLAST of Lag0025721 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 740.0 bits (1909), Expect = 3.1e-212
Identity = 401/769 (52.15%), Postives = 517/769 (67.23%), Query Frame = 0
Query: 96 SRHINNAYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYS 155
S+HI A K SYVV G+HSH E++E + RV +HY LGSF GS ++A DAIFYS
Sbjct: 20 SKHI-LASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYS 79
Query: 156 YKKHINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLV 215
Y KHINGFAA L+ + A E++KH EV V N+ ++LHTT S + ++ V
Sbjct: 80 YTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS------YV 139
Query: 216 PYTFMALHA-FGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNR 275
P + + A FGE+ +NLD+GVWPES SF+D+G+ PIPS+WKG C+N + FHCNR
Sbjct: 140 PSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLG-PIPSRWKGICQNQKDATFHCNR 199
Query: 276 KLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSS 335
KLIGA++FN+GY A + +N++FD S D GHGSHTLSTA G+FV +FG
Sbjct: 200 KLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPGVSIFG-Q 259
Query: 336 GFGSAKGGSPRARVAAYKVCWGDNVG--CPTADILQGVDEAIHDGVDVISYSVGGGPSPF 395
G G+AKGGSPRARVAAYKVCW G C AD+L D AIHDG DVIS S+GG P+ F
Sbjct: 260 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 319
Query: 396 YQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNN 455
+ D AI SFHA K I VVCSAGNSGP + N+APW +TVGAST+DR AS + L N
Sbjct: 320 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 379
Query: 456 GQSFQGESLSK-GLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLR 515
G+ ++G+SLS LP K+YP+++ A A A + DA LCK G+LDP K KGKIL CLR
Sbjct: 380 GKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLR 439
Query: 516 GETARIDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQ 575
G+ R++KG G +GMVL N G +LAD HVLPA Q+ +D AV YI T+
Sbjct: 440 GQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTK 499
Query: 576 NPSAYIVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSD 635
P A+I P + PAP+MA FSS+GP+++ P+I+KPDITAPGV++IAA T A SP++
Sbjct: 500 KPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTN 559
Query: 636 DPSHNRKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPM 695
+ R+ + ++SGTSMSCPHISG+ GLLKT +P WSPAAI+SAIMT+A + D+ P+
Sbjct: 560 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPI 619
Query: 696 QDGGSERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDE 755
Q+ + + ATPFS+G+GHVQP A++PGLVYDL +DYL+FLC+LGY+ S + VFS
Sbjct: 620 QNATNMK---ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN 679
Query: 756 AYQC-PPSASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEP 815
+ C P SL+N NYPSI V NL + +VTV+R +KNVG P Y V P+GV V V+P
Sbjct: 680 NFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKP 739
Query: 816 RVLKFDQVGEEKSFTVTL--SGASPLNAVVDGILIWSDGKHFVSSPIVV 857
L F +VGE+K+F V L S + V G L+WSD KH V SPIVV
Sbjct: 740 TSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVV 770
BLAST of Lag0025721 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 597.0 bits (1538), Expect = 3.3e-169
Identity = 352/789 (44.61%), Postives = 466/789 (59.06%), Query Frame = 0
Query: 105 TKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHINGFA 164
+KK Y+V +G+HSHG + ADL+ T+SHY LLGS GS +KAK+AI YSY +HINGFA
Sbjct: 28 SKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFA 87
Query: 165 AMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMALH- 224
A+LEEEEAA++AK+ V V ++ +LHTT S + F+ LH
Sbjct: 88 ALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRS-------------------WEFLGLHR 147
Query: 225 ----------AFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSC----ENPHNNG 284
FGE N+D+GVWPES SF D G +PSKW+G + P +
Sbjct: 148 RGQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYG-TVPSKWRGGLCQINKLPGSMK 207
Query: 285 FHCNRKLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKD 344
CNRKLIGA+++N+ + A+ N + ++++ D+ GHG+HTLSTAGGNFV
Sbjct: 208 NTCNRKLIGARYYNKAFEAH------NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGAR 267
Query: 345 VFGSSGFGSAKGGSPRARVAAYKVCWG--DNVGCPTADILQGVDEAIHDGVDVISYSVGG 404
VF + G G+AKGGSPRARVAAYKVCW D C AD+L +D+AI DGVDVI+ S G
Sbjct: 268 VF-AVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGV 327
Query: 405 G----PSPFYQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRR 464
+ D +I +FHA+ I +V SAGN GP PG+ N+APW+ T+ ASTLDR
Sbjct: 328 SYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRD 387
Query: 465 IASVVQLNNGQSFQGESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVK 524
+S + +NN Q +G SL LP + + LI A A A DA LC+ GTLD KV
Sbjct: 388 FSSNLTINN-QLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVN 447
Query: 525 GKILACLR-GETARIDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLA 584
GKI+ C R G+ + +G++A AGA GM+L N NG+ + A+ HV + +
Sbjct: 448 GKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKS 507
Query: 585 VFNYIDNT----------QNPSAYIVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDI 644
+ + T + + F PAP+MA FSSRGPN I P I+KPD+
Sbjct: 508 RPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDV 567
Query: 645 TAPGVNIIAATTQAHSPSDDPSHNRK-TPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPA 704
TAPGVNI+AA ++ S S NR+ + + GTSMSCPH SG+ GLLKT HP WSPA
Sbjct: 568 TAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPA 627
Query: 705 AIKSAIMTSARVRDNTMHPMQDGGSERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDY 764
AIKSAIMT+A DNT P+QD + LA F+YGSGHV+P A++PGLVYDL+ DY
Sbjct: 628 AIKSAIMTTATTLDNTNRPIQDAFDK--TLADAFAYGSGHVRPDLAIEPGLVYDLSLTDY 687
Query: 765 LDFLCALGYDESMMRVFS-DEAYQCPPSASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGS 824
L+FLCA GYD+ ++ + + + C S S+ + NYPSI + NL VT+ R + NVG
Sbjct: 688 LNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGP 747
Query: 825 PGEYKASVRRPEGVNVVVEPRVLKFDQVGEEKSFTVTL--SGASPLNAVVDGILIWSDGK 857
P Y S R P G ++ V P L F ++GE K+F V + S A+ G L W+DGK
Sbjct: 748 PSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGK 786
BLAST of Lag0025721 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 560.1 bits (1442), Expect = 4.4e-158
Identity = 327/714 (45.80%), Postives = 428/714 (59.94%), Query Frame = 0
Query: 152 IFYSYKKHINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLC 211
+ Y+Y+ I+GF+ L +EEA L V VL ELHTT + + H DL
Sbjct: 65 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL- 124
Query: 212 RVLVPYTFMALHAFGEECFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHN-NGFH 271
F ++ + LD+GVWPES S+ D+G PIPS WKG CE N
Sbjct: 125 -------FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFG-PIPSSWKGGCEAGTNFTASL 184
Query: 272 CNRKLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVF 331
CNRKLIGA+FF GY + M I D S S D GHG+HT STA G+ V +
Sbjct: 185 CNRKLIGARFFARGYESTMGPI----DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 244
Query: 332 GSSGFGSAKGGSPRARVAAYKVCWGDNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSP 391
G + G+A+G +PRARVA YKVCW GC ++DIL +D+AI D V+V+S S+GGG S
Sbjct: 245 GYAS-GTARGMAPRARVAVYKVCWLG--GCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 304
Query: 392 FYQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLN 451
+Y+DG AI +F A++ GI V CSAGN+GP S N+APW+ TVGA TLDR ++ L
Sbjct: 305 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 364
Query: 452 NGQSFQGESLSKG--LPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILAC 511
NG++F G SL KG LP +K P I G A+ A + LC GTL P KVKGKI+ C
Sbjct: 365 NGKNFTGVSLFKGEALP-DKLLPFIYAGNASNA----TNGNLCMTGTLIPEKVKGKIVMC 424
Query: 512 LRGETARIDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDN 571
RG AR+ KG + AG VGM+L N NGE+++AD H+LPA +G + G + +Y+
Sbjct: 425 DRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT 484
Query: 572 TQNPSAYIVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSP 631
NP+A I V P+P++A FSSRGPN ITP I+KPD+ APGVNI+AA T A P
Sbjct: 485 DPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGP 544
Query: 632 SDDPSHNRKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMH 691
+ S +R+ + +SGTSMSCPH+SG+ LLK++HP+WSPAAI+SA+MT+A
Sbjct: 545 TGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 604
Query: 692 PMQDGGSERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFS 751
P+ D + G+ +TPF +G+GHV P A +PGL+YDLTTEDYL FLCAL Y +R S
Sbjct: 605 PLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVS 664
Query: 752 DEAYQCPPSA--SLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASV-RRPEGVNV 811
Y C PS S+ + NYPS AV G TR + +VG G Y V GV +
Sbjct: 665 RRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKI 724
Query: 812 VVEPRVLKFDQVGEEKSFTV--TLSGASPLNAVVDGILIWSDGKHFVSSPIVVS 858
VEP VL F + E+KS+TV T+ + P + G + WSDGKH V SP+ +S
Sbjct: 725 SVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of Lag0025721 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 506.5 bits (1303), Expect = 5.8e-142
Identity = 306/750 (40.80%), Postives = 421/750 (56.13%), Query Frame = 0
Query: 133 SHYKLLGSFLGSNKKAKDAIFYSYKKHINGFAAMLEEEEAAELAKHREVAVVLGNEMMEL 192
+H+ S L S + +I ++Y +GF+A L ++A++L H V V+ ++ L
Sbjct: 43 THFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHL 102
Query: 193 HTTHSVGEWTISYHCGDLCRVLVPYTFMALHAFGEECFSNLDSGVWPESMSFKDDGINKP 252
HTT S + D +L F + G +D+GVWPE SF D G+ P
Sbjct: 103 HTTRS--PEFLGLRSTDKAGLLEESDFGSDLVIGV-----IDTGVWPERPSFDDRGLG-P 162
Query: 253 IPSKWKGSC----ENPHNNGFHCNRKLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDY 312
+P KWKG C + P + CNRKL+GA+FF GY + N + ++ S D
Sbjct: 163 VPIKWKGQCIASQDFPES---ACNRKLVGARFFCGGY----EATNGKMNETTEFRSPRDS 222
Query: 313 AGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKGGSPRARVAAYKVCWGDNVGCPTADILQG 372
GHG+HT S + G +V P G + G A G +P+AR+AAYKVCW N GC +DIL
Sbjct: 223 DGHGTHTASISAGRYVFPASTLGYA-HGVAAGMAPKARLAAYKVCW--NSGCYDSDILAA 282
Query: 373 VDEAIHDGVDVISYSVGGGPSPFYQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLA 432
D A+ DGVDVIS SVGG P+Y D AI +F A+ GI V SAGN GP + N+A
Sbjct: 283 FDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVA 342
Query: 433 PWMLTVGASTLDRRIASVVQLNNGQSFQGESL--SKGLPEEKYYPLISGGQAAAAGAYSG 492
PWM TVGA T+DR + V+L NG+ G S+ GL + YPL+ GG YS
Sbjct: 343 PWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS- 402
Query: 493 DAMLCKPGTLDPAKVKGKILACLRGETARIDKGIQAENAGAVGMVLCNDEGNGEQILADF 552
+ LC G+LDP VKGKI+ C RG +R KG G +GM++ N +GE ++AD
Sbjct: 403 -SSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADC 462
Query: 553 HVLPAIQIGYEDGLAVFNYID------NTQNPSAYIVPPTEKFDVTPAPIMAKFSSRGPN 612
HVLPA +G G + YI ++++P+A IV + + PAP++A FS+RGPN
Sbjct: 463 HVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPN 522
Query: 613 LITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPYVSMSGTSMSCPHISGVVGLL 672
TPEI+KPD+ APG+NI+AA PS S NR+T + +SGTSM+CPH+SG+ LL
Sbjct: 523 PETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALL 582
Query: 673 KTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGELATPFSYGSGHVQPVGAMDPG 732
K HPDWSPAAI+SA++T+A DN+ PM D G ++ YGSGHV P AMDPG
Sbjct: 583 KAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVMDYGSGHVHPTKAMDPG 642
Query: 733 LVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCP---PSASLLNFNYPSIAV--QNLNN 792
LVYD+T+ DY++FLC Y + + + C + + N NYPS +V Q
Sbjct: 643 LVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGE 702
Query: 793 GRVTV--TRKLKNVG-SPGEYKASVRRPEGVNVVVEPRVLKFDQVGEEKSFTVTLSGA-- 852
+++ R + NVG S Y+ +R P G V VEP L F +VG++ SF V +
Sbjct: 703 SKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEV 762
Query: 853 --SP-LNAVVDGILIWSDGKHFVSSPIVVS 858
SP V G ++WSDGK V+SP+VV+
Sbjct: 763 KLSPGATNVETGHIVWSDGKRNVTSPLVVT 770
BLAST of Lag0025721 vs. ExPASy TrEMBL
Match:
A0A6J1CLZ9 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012693 PE=3 SV=1)
HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 588/762 (77.17%), Postives = 651/762 (85.43%), Query Frame = 0
Query: 102 AYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHIN 161
++ KK YVVLLGSHSHGLE+SE DLQRV NSHY+LLGS LGS KKAKDAIFYSYKKHIN
Sbjct: 25 SFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYSYKKHIN 84
Query: 162 GFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMA 221
GFAA+LEEE+AA LAKH EVAVVL +EMM+LHTTHS G + +P +
Sbjct: 85 GFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSS-----IPSSAWG 144
Query: 222 LHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKF 281
FGE+ +NLDSGVWPESMSF+D GI KPIPS+WKGSCE GF CNRKLIGAKF
Sbjct: 145 KARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCE----QGFPCNRKLIGAKF 204
Query: 282 FNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKG 341
+NEGY+AYMR++N NF+VS+V NS D AGHGSHTLSTAGGNFVA K+VFGSSGFG AKG
Sbjct: 205 YNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKG 264
Query: 342 GSPRARVAAYKVCWGDNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIAS 401
GSP+AR+AAYKVCWG NVGCP ADILQG+D+AIHDGVDV+SYS+GG P+YQD FAIAS
Sbjct: 265 GSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVIVPYYQDFFAIAS 324
Query: 402 FHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGESL 461
F AVKNGISVVCSAGN+GP PG+AVNLAPW+ TVGASTLDRRI + VQLNNGQ FQG SL
Sbjct: 325 FQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSL 384
Query: 462 SKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARIDKGI 521
SKGLPEEKYYPLISGGQAAA GAY DAMLCK G LDPAKVKGKILAC+RGETARIDKG
Sbjct: 385 SKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGT 444
Query: 522 QAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIVPPT 581
+A AGAVGM+LCND+ +GE +D HVLPA IGY+ LAVF+Y+DNTQNP AY+VPP+
Sbjct: 445 EAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPS 504
Query: 582 EKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPY 641
EKFD+ PAP MA FSSRGPN+ITPEIIKPDITAPGVNIIAA TQ+H PS+DPS R+ PY
Sbjct: 505 EKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPY 564
Query: 642 VSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGEL 701
++MSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMT+ARVRDNTM PM DGGS RG+
Sbjct: 565 MTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDP 624
Query: 702 ATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEA-YQCPPSAS 761
ATPFSYGSGHVQPV AMDPGLVYDLTTEDYL+FLCALGYD SM+R FSD+ Y CPPSAS
Sbjct: 625 ATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSAS 684
Query: 762 LLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQVGE 821
LL+FNYPSI VQNL NG+VTVTRKLKNVGSPG Y+ASVRRPEGV V VEPRVL+F+ VGE
Sbjct: 685 LLDFNYPSIGVQNL-NGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGE 744
Query: 822 EKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
EKSF+VTL+G P NAVVDG LIWSDGKHFV SPIVVSSG F
Sbjct: 745 EKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 776
BLAST of Lag0025721 vs. ExPASy TrEMBL
Match:
A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)
HSP 1 Score: 1000.0 bits (2584), Expect = 6.2e-288
Identity = 500/766 (65.27%), Postives = 607/766 (79.24%), Query Frame = 0
Query: 99 INNAYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKK 158
I+ A KKSYVV+LGSHSHGLEVSEADLQRV +SH+KLLGSFLGS++KAKDAIFYSYKK
Sbjct: 21 ISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKK 80
Query: 159 HINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYT 218
+INGFAA+LEEEEAAELAKH EVA VL N+ +LHTTHS + G +VP T
Sbjct: 81 NINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNG-----VVPPT 140
Query: 219 FMALHA-FGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLI 278
A FG++ +NLD+GVWPES SF ++GI +P+KWKG C + + CNRKLI
Sbjct: 141 SPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRKLI 200
Query: 279 GAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFG 338
GAK+FN+G+IAY++++N++ D+SS+ NST DY GHGSHTLSTAGG+FV VFG SG G
Sbjct: 201 GAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFG-SGLG 260
Query: 339 SAKGGSPRARVAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDG 398
+AKGGSP+ARVAAYK+CW + GC ADI +G D AIHDGV+VIS SVGG P+ ++ D
Sbjct: 261 TAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDDS 320
Query: 399 FAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSF 458
AIA+FHAVK GI VVCSAGNSGPD +A N APW+LTVGASTLDR+ + V+L NGQ F
Sbjct: 321 IAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQRF 380
Query: 459 QGESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETAR 518
QG SLS LPE+K YPLI+G QA A A + DAMLCKP TLD +K KGKILACLRG+ AR
Sbjct: 381 QGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNAR 440
Query: 519 IDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAY 578
+DKG QA AGA GM+LCNDE +G + +AD H+LPA + Y DG AV YI +TQNP Y
Sbjct: 441 VDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMGY 500
Query: 579 IVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHN 638
++PP K + PAP+MA FSSRGPN++TPEIIKPD+TAPGVN+IAA T+A SP+ +P N
Sbjct: 501 LIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFDN 560
Query: 639 RKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGS 698
R P+++MSGTSMSCPH++G+ GLL++LHP+WSP+AIKSAIMTSAR+RDNTM PM DGG+
Sbjct: 561 RTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGT 620
Query: 699 ERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCP 758
+ ATPFSYGSGH++P+GA+DPGLVYDLT +DYL+FLCA+GYDE +R FSD Y+CP
Sbjct: 621 DELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCP 680
Query: 759 PSASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFD 818
PSASLLNFNYPSI VQN+ G VTVTR+LKNVG+PG Y+A VR+PEGV V VEPR LKFD
Sbjct: 681 PSASLLNFNYPSIGVQNM-TGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFD 740
Query: 819 QVGEEKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
+VGEEKSF +T++G P VVDG LIW+DG+HFV SPIV+SSG F
Sbjct: 741 KVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779
BLAST of Lag0025721 vs. ExPASy TrEMBL
Match:
A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)
HSP 1 Score: 933.7 bits (2412), Expect = 5.5e-268
Identity = 473/756 (62.57%), Postives = 576/756 (76.19%), Query Frame = 0
Query: 108 SYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHINGFAAML 167
SYVVLLGSHSHGLEV+E D + V +SH+KLLGSFL S++KAKD+IFYSYKKHINGFAA L
Sbjct: 755 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814
Query: 168 EEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMALHAFGE 227
++E+A LA H EVA VL N+ +L+TTHS W + + V+ P + + FG+
Sbjct: 815 DDEDATRLANHPEVAAVLPNKPKDLYTTHS---WEFMHL--EKNGVVPPSSPWRMAKFGK 874
Query: 228 E-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKFFNEGYI 287
+ +NLD+GVWPES SF + GI+ P PSKWKG C + +G CN+KLIGAK+FN+GY+
Sbjct: 875 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYL 934
Query: 288 AYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKGGSPRAR 347
Y++S N+ D+SS+INST DY GHGSHTLSTA GN+V VFG SG G+AKGGSP+AR
Sbjct: 935 EYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFG-SGIGTAKGGSPKAR 994
Query: 348 VAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIASFHAVK 407
VAAYKVCW + GC ADI + D AIHDGVDV+S S+GG P + +D AIASFHAVK
Sbjct: 995 VAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVK 1054
Query: 408 NGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGESLSKGLP 467
GI VVC+ GNSGP P +A N APW+LTVGASTLDR + V L NG F G S SKGL
Sbjct: 1055 KGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLT 1114
Query: 468 EEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARIDKGIQAENA 527
K YPLI+G QA A A DAMLCKP TLD +KVKGKIL CLRGETAR+DKG QA A
Sbjct: 1115 GRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 1174
Query: 528 GAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIVPPTEKFDV 587
GAVGM+LCND+ +G I+ DFH+LPA I Y+DG + +YI++ +NP Y++PP K +
Sbjct: 1175 GAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNT 1234
Query: 588 TPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPYVSMSG 647
PAP MA FSSRGPN I+PEIIKPD+TAPGVNIIAA ++A SP+ D S NR TP+++MSG
Sbjct: 1235 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSG 1294
Query: 648 TSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGELATPFS 707
TSMSCPH++G+VGLL+ LHPDWSP+AIKSAIMTS++VRDNT++PM DGGS ATPF+
Sbjct: 1295 TSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFA 1354
Query: 708 YGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCPPSASLLNFNY 767
YGSGH+ P GA+DPGLVYDL+ DYL+FLCA GYDE +R FSDE ++CPP++S+LN NY
Sbjct: 1355 YGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNY 1414
Query: 768 PSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQVGEEKSFTV 827
PSI VQNL G V+VTRKLKNVGSPG Y+A + P GV V V+PR LKF++VGEEKSF +
Sbjct: 1415 PSIGVQNL-KGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFEL 1474
Query: 828 TLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
TL+G P + V G LIWSDG+H V SPIVVSSG F
Sbjct: 1475 TLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503
BLAST of Lag0025721 vs. ExPASy TrEMBL
Match:
A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)
HSP 1 Score: 929.1 bits (2400), Expect = 1.3e-266
Identity = 471/757 (62.22%), Postives = 582/757 (76.88%), Query Frame = 0
Query: 107 KSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHINGFAAM 166
+SY+VLLGSHSHGLEV++ DL+RV +SH+KLLGS GS++KA++AIFYSYKK+INGFAA+
Sbjct: 11 QSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAI 70
Query: 167 LEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMALHAFG 226
++EEEAA+LAKH EVA VL N +LHTTHS W + + V+ P + G
Sbjct: 71 MDEEEAAQLAKHPEVAAVLPNRAKKLHTTHS---WEFMHL--EKNGVIPPSSAWRRAKSG 130
Query: 227 EE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKFFNEGY 286
++ +NLD+GVWPES SF + GI P+PSKWKG C + + CNRKLIGAK+FN+G+
Sbjct: 131 KDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGF 190
Query: 287 IAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKGGSPRA 346
+AY++S N + VINST DY GHGSHTLSTAGG++V+ VFG G G+AKGGSP+A
Sbjct: 191 LAYLKSENL---TALVINSTRDYDGHGSHTLSTAGGSYVSGASVFG-LGVGTAKGGSPKA 250
Query: 347 RVAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIASFHAV 406
RVAAYKVCW ++ GC ADI Q D AIHD VDV+S S+GG P+ +Y DG AI++FHAV
Sbjct: 251 RVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAV 310
Query: 407 KNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGESLSKGL 466
K GI VVCSAGNSGP + N APW+LTVGAST+DR + V+L NG + G SLSKGL
Sbjct: 311 KKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGL 370
Query: 467 PEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARIDKGIQAEN 526
+K YPLI+G +A A A + +A LCKP TLD +KVKGKIL CLRG+TAR+DKG QA
Sbjct: 371 KGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAAL 430
Query: 527 AGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIVPPTEKFD 586
AGAVGM+LCNDE +G + +AD HVLPA I Y DG AVF+YI T+NP Y++PPT K +
Sbjct: 431 AGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVN 490
Query: 587 VTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPYVSMS 646
PAP MA FSSRGPNLI+PEIIKPD+TAPGVNIIAA ++A SP+ +P NR P+++MS
Sbjct: 491 TKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMS 550
Query: 647 GTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGELATPF 706
GTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMTSAR+RDNT PM DGGS +TPF
Sbjct: 551 GTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPF 610
Query: 707 SYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCPPSASLLNFN 766
+YGSGH++P GA+DPGLVYDL+ DYL+FLCA GY+E ++ FSD ++CP SAS+LN N
Sbjct: 611 AYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLN 670
Query: 767 YPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQVGEEKSFT 826
YPSI VQNL G VTVTRKLKNV +PG YK VR P GV V+V+P+VLKF++VGEEKSF
Sbjct: 671 YPSIGVQNL-TGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFE 730
Query: 827 VTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
+T++G P + VVDG+LIW+DGKHFV SPIVVSS F
Sbjct: 731 LTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757
BLAST of Lag0025721 vs. ExPASy TrEMBL
Match:
A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)
HSP 1 Score: 928.3 bits (2398), Expect = 2.3e-266
Identity = 474/765 (61.96%), Postives = 585/765 (76.47%), Query Frame = 0
Query: 99 INNAYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKK 158
I+ A KKSY+VLLGSHSHGLEV + DL+RV +SH+KLLGS +GS++KA+DAIFYSYK+
Sbjct: 22 ISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSIIGSDEKARDAIFYSYKR 81
Query: 159 HINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYT 218
+INGFAA+++EEEA +LAKH EVA VL N+ +LHTTHS W + + V+ P +
Sbjct: 82 NINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHS---WEFMHL--EKNGVIPPSS 141
Query: 219 FMALHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIG 278
G++ NLD+GVW ES SF + GI +PSKWKG C + +G CNRKLIG
Sbjct: 142 AWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTPDGVSCNRKLIG 201
Query: 279 AKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGS 338
AK+FN+G++AY+ S N +SVINST DY GHGSHTLSTAGG++V+ VFG G G+
Sbjct: 202 AKYFNKGFLAYLNSQNL---TASVINSTRDYDGHGSHTLSTAGGSYVSGASVFG-LGVGT 261
Query: 339 AKGGSPRARVAAYKVCWG-DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGF 398
AKGGSP+ARVA+YKVCW ++ GC ADI + D AIHD VDV+S S+GG P+ +Y DG
Sbjct: 262 AKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGI 321
Query: 399 AIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQ 458
AIA+FHAVK GI VVCSAGNSGP + N APW+LTVGASTLDR + V+L NG S+
Sbjct: 322 AIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAPVELQNGHSYM 381
Query: 459 GESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARI 518
G SLSKGL +K YPLI+G +A A A + AMLCKP TLD +KVKGKIL CLRG+TAR+
Sbjct: 382 GSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARV 441
Query: 519 DKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYI 578
DKG QA AGAVGM+LCND+ +G + +AD HVLPA I Y DG AVF+YI +T+NP +
Sbjct: 442 DKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGSL 501
Query: 579 VPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNR 638
+PP+ K + PAP MA FSSRGPNLI+PEIIKPD+TAPGVNIIAA ++A SP+ +P NR
Sbjct: 502 IPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNR 561
Query: 639 KTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSE 698
P+++MSGTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMTSAR+RDNT PM DGGS
Sbjct: 562 TVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTKKPMLDGGSP 621
Query: 699 RGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCPP 758
ATPF+YGSGH++P GA+DPGLVYDL+ DYL+FLCA GY+E ++ FSD ++CP
Sbjct: 622 DLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPA 681
Query: 759 SASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQ 818
SAS+LNFNYPSI VQNL G VT+TRKLKNV +PG YKA V P GV V+V+P+VLKF++
Sbjct: 682 SASILNFNYPSIGVQNL-TGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFER 741
Query: 819 VGEEKSFTVTLSGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
VGEEK F + ++G P N VVDG+LIW+DGKHFV SPIVVSSG F
Sbjct: 742 VGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776
BLAST of Lag0025721 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 805.4 bits (2079), Expect = 4.3e-233
Identity = 433/764 (56.68%), Postives = 536/764 (70.16%), Query Frame = 0
Query: 102 AYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHIN 161
A+ KKSY+V LGSH+H ++S A L V +SH L SF+GS++ AK+AIFYSYK+HIN
Sbjct: 35 AFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHIN 94
Query: 162 GFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMA 221
GFAA+L+E EAAE+AKH +V V N+ +LHTTHS W + V+ +
Sbjct: 95 GFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHS---WNFMLLAKN--GVVHKSSLWN 154
Query: 222 LHAFGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNRKLIGAKF 281
+GE+ +NLD+GVWPES SF D+G +P++WKG C + CNRKLIGA++
Sbjct: 155 KAGYGEDTIIANLDTGVWPESKSFSDEGYG-AVPARWKGRC----HKDVPCNRKLIGARY 214
Query: 282 FNEGYIAYM-RSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAK 341
FN+GY+AY NA+++ + D+ GHGSHTLSTA GNFV +VFG G G+A
Sbjct: 215 FNKGYLAYTGLPSNASYE------TCRDHDGHGSHTLSTAAGNFVPGANVFG-IGNGTAS 274
Query: 342 GGSPRARVAAYKVCWG--DNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFA 401
GGSP+ARVAAYKVCW D C ADIL ++ AI DGVDV+S SVGG + DG A
Sbjct: 275 GGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIA 334
Query: 402 IASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQG 461
I SFHAVKNG++VVCSAGNSGP G+ N+APW++TVGAS++DR + V+L NGQSF+G
Sbjct: 335 IGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 394
Query: 462 ESLSKGLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETARID 521
SLSK LPEEK Y LIS A A DA+LCK G+LDP KVKGKIL CLRG+ AR+D
Sbjct: 395 TSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVD 454
Query: 522 KGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSAYIV 581
KG+QA AGA GMVLCND+ +G +I++D HVLPA QI Y+DG +F+Y+ +T++P YI
Sbjct: 455 KGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIK 514
Query: 582 PPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRK 641
PT + PAP MA FSSRGPN ITP I+KPDITAPGVNIIAA T+A P+D S NR+
Sbjct: 515 APTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRR 574
Query: 642 TPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSER 701
TP+ + SGTSMSCPHISGVVGLLKTLHP WSPAAI+SAIMT++R R+N PM D E
Sbjct: 575 TPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVD---ES 634
Query: 702 GELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFS-DEAYQCPP 761
+ A PFSYGSGHVQP A PGLVYDLTT DYLDFLCA+GY+ +++++F+ D Y C
Sbjct: 635 FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ 694
Query: 762 SASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEPRVLKFDQ 821
A+LL+FNYPSI V NL G +TVTRKLKNVG P Y A R P GV V VEP+ L F++
Sbjct: 695 GANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 754
Query: 822 VGEEKSFTVTLSGASPL----NAVVDGILIWSDGKHFVSSPIVV 857
GE K F +TL PL + V G L W+D H+V SPIVV
Sbjct: 755 TGEVKIFQMTL---RPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774
BLAST of Lag0025721 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 740.0 bits (1909), Expect = 2.2e-213
Identity = 401/769 (52.15%), Postives = 517/769 (67.23%), Query Frame = 0
Query: 96 SRHINNAYKTKKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYS 155
S+HI A K SYVV G+HSH E++E + RV +HY LGSF GS ++A DAIFYS
Sbjct: 20 SKHI-LASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYS 79
Query: 156 YKKHINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLV 215
Y KHINGFAA L+ + A E++KH EV V N+ ++LHTT S + ++ V
Sbjct: 80 YTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS------YV 139
Query: 216 PYTFMALHA-FGEE-CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHNNGFHCNR 275
P + + A FGE+ +NLD+GVWPES SF+D+G+ PIPS+WKG C+N + FHCNR
Sbjct: 140 PSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLG-PIPSRWKGICQNQKDATFHCNR 199
Query: 276 KLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSS 335
KLIGA++FN+GY A + +N++FD S D GHGSHTLSTA G+FV +FG
Sbjct: 200 KLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPGVSIFG-Q 259
Query: 336 GFGSAKGGSPRARVAAYKVCWGDNVG--CPTADILQGVDEAIHDGVDVISYSVGGGPSPF 395
G G+AKGGSPRARVAAYKVCW G C AD+L D AIHDG DVIS S+GG P+ F
Sbjct: 260 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 319
Query: 396 YQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNN 455
+ D AI SFHA K I VVCSAGNSGP + N+APW +TVGAST+DR AS + L N
Sbjct: 320 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 379
Query: 456 GQSFQGESLSK-GLPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLR 515
G+ ++G+SLS LP K+YP+++ A A A + DA LCK G+LDP K KGKIL CLR
Sbjct: 380 GKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLR 439
Query: 516 GETARIDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQ 575
G+ R++KG G +GMVL N G +LAD HVLPA Q+ +D AV YI T+
Sbjct: 440 GQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTK 499
Query: 576 NPSAYIVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSD 635
P A+I P + PAP+MA FSS+GP+++ P+I+KPDITAPGV++IAA T A SP++
Sbjct: 500 KPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTN 559
Query: 636 DPSHNRKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPM 695
+ R+ + ++SGTSMSCPHISG+ GLLKT +P WSPAAI+SAIMT+A + D+ P+
Sbjct: 560 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPI 619
Query: 696 QDGGSERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFSDE 755
Q+ + + ATPFS+G+GHVQP A++PGLVYDL +DYL+FLC+LGY+ S + VFS
Sbjct: 620 QNATNMK---ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN 679
Query: 756 AYQC-PPSASLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASVRRPEGVNVVVEP 815
+ C P SL+N NYPSI V NL + +VTV+R +KNVG P Y V P+GV V V+P
Sbjct: 680 NFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKP 739
Query: 816 RVLKFDQVGEEKSFTVTL--SGASPLNAVVDGILIWSDGKHFVSSPIVV 857
L F +VGE+K+F V L S + V G L+WSD KH V SPIVV
Sbjct: 740 TSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVV 770
BLAST of Lag0025721 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 560.1 bits (1442), Expect = 3.2e-159
Identity = 327/714 (45.80%), Postives = 428/714 (59.94%), Query Frame = 0
Query: 152 IFYSYKKHINGFAAMLEEEEAAELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLC 211
+ Y+Y+ I+GF+ L +EEA L V VL ELHTT + + H DL
Sbjct: 65 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL- 124
Query: 212 RVLVPYTFMALHAFGEECFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHN-NGFH 271
F ++ + LD+GVWPES S+ D+G PIPS WKG CE N
Sbjct: 125 -------FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFG-PIPSSWKGGCEAGTNFTASL 184
Query: 272 CNRKLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVF 331
CNRKLIGA+FF GY + M I D S S D GHG+HT STA G+ V +
Sbjct: 185 CNRKLIGARFFARGYESTMGPI----DESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 244
Query: 332 GSSGFGSAKGGSPRARVAAYKVCWGDNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSP 391
G + G+A+G +PRARVA YKVCW GC ++DIL +D+AI D V+V+S S+GGG S
Sbjct: 245 GYAS-GTARGMAPRARVAVYKVCWLG--GCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 304
Query: 392 FYQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLN 451
+Y+DG AI +F A++ GI V CSAGN+GP S N+APW+ TVGA TLDR ++ L
Sbjct: 305 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 364
Query: 452 NGQSFQGESLSKG--LPEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILAC 511
NG++F G SL KG LP +K P I G A+ A + LC GTL P KVKGKI+ C
Sbjct: 365 NGKNFTGVSLFKGEALP-DKLLPFIYAGNASNA----TNGNLCMTGTLIPEKVKGKIVMC 424
Query: 512 LRGETARIDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDN 571
RG AR+ KG + AG VGM+L N NGE+++AD H+LPA +G + G + +Y+
Sbjct: 425 DRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT 484
Query: 572 TQNPSAYIVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSP 631
NP+A I V P+P++A FSSRGPN ITP I+KPD+ APGVNI+AA T A P
Sbjct: 485 DPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGP 544
Query: 632 SDDPSHNRKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMH 691
+ S +R+ + +SGTSMSCPH+SG+ LLK++HP+WSPAAI+SA+MT+A
Sbjct: 545 TGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 604
Query: 692 PMQDGGSERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYDESMMRVFS 751
P+ D + G+ +TPF +G+GHV P A +PGL+YDLTTEDYL FLCAL Y +R S
Sbjct: 605 PLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVS 664
Query: 752 DEAYQCPPSA--SLLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGEYKASV-RRPEGVNV 811
Y C PS S+ + NYPS AV G TR + +VG G Y V GV +
Sbjct: 665 RRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKI 724
Query: 812 VVEPRVLKFDQVGEEKSFTV--TLSGASPLNAVVDGILIWSDGKHFVSSPIVVS 858
VEP VL F + E+KS+TV T+ + P + G + WSDGKH V SP+ +S
Sbjct: 725 SVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of Lag0025721 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 506.5 bits (1303), Expect = 4.1e-143
Identity = 306/750 (40.80%), Postives = 421/750 (56.13%), Query Frame = 0
Query: 133 SHYKLLGSFLGSNKKAKDAIFYSYKKHINGFAAMLEEEEAAELAKHREVAVVLGNEMMEL 192
+H+ S L S + +I ++Y +GF+A L ++A++L H V V+ ++ L
Sbjct: 43 THFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHL 102
Query: 193 HTTHSVGEWTISYHCGDLCRVLVPYTFMALHAFGEECFSNLDSGVWPESMSFKDDGINKP 252
HTT S + D +L F + G +D+GVWPE SF D G+ P
Sbjct: 103 HTTRS--PEFLGLRSTDKAGLLEESDFGSDLVIGV-----IDTGVWPERPSFDDRGLG-P 162
Query: 253 IPSKWKGSC----ENPHNNGFHCNRKLIGAKFFNEGYIAYMRSINANFDVSSVINSTHDY 312
+P KWKG C + P + CNRKL+GA+FF GY + N + ++ S D
Sbjct: 163 VPIKWKGQCIASQDFPES---ACNRKLVGARFFCGGY----EATNGKMNETTEFRSPRDS 222
Query: 313 AGHGSHTLSTAGGNFVAPKDVFGSSGFGSAKGGSPRARVAAYKVCWGDNVGCPTADILQG 372
GHG+HT S + G +V P G + G A G +P+AR+AAYKVCW N GC +DIL
Sbjct: 223 DGHGTHTASISAGRYVFPASTLGYA-HGVAAGMAPKARLAAYKVCW--NSGCYDSDILAA 282
Query: 373 VDEAIHDGVDVISYSVGGGPSPFYQDGFAIASFHAVKNGISVVCSAGNSGPDPGSAVNLA 432
D A+ DGVDVIS SVGG P+Y D AI +F A+ GI V SAGN GP + N+A
Sbjct: 283 FDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVA 342
Query: 433 PWMLTVGASTLDRRIASVVQLNNGQSFQGESL--SKGLPEEKYYPLISGGQAAAAGAYSG 492
PWM TVGA T+DR + V+L NG+ G S+ GL + YPL+ GG YS
Sbjct: 343 PWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS- 402
Query: 493 DAMLCKPGTLDPAKVKGKILACLRGETARIDKGIQAENAGAVGMVLCNDEGNGEQILADF 552
+ LC G+LDP VKGKI+ C RG +R KG G +GM++ N +GE ++AD
Sbjct: 403 -SSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADC 462
Query: 553 HVLPAIQIGYEDGLAVFNYID------NTQNPSAYIVPPTEKFDVTPAPIMAKFSSRGPN 612
HVLPA +G G + YI ++++P+A IV + + PAP++A FS+RGPN
Sbjct: 463 HVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPN 522
Query: 613 LITPEIIKPDITAPGVNIIAATTQAHSPSDDPSHNRKTPYVSMSGTSMSCPHISGVVGLL 672
TPEI+KPD+ APG+NI+AA PS S NR+T + +SGTSM+CPH+SG+ LL
Sbjct: 523 PETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALL 582
Query: 673 KTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGGSERGELATPFSYGSGHVQPVGAMDPG 732
K HPDWSPAAI+SA++T+A DN+ PM D G ++ YGSGHV P AMDPG
Sbjct: 583 KAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVMDYGSGHVHPTKAMDPG 642
Query: 733 LVYDLTTEDYLDFLCALGYDESMMRVFSDEAYQCP---PSASLLNFNYPSIAV--QNLNN 792
LVYD+T+ DY++FLC Y + + + C + + N NYPS +V Q
Sbjct: 643 LVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGE 702
Query: 793 GRVTV--TRKLKNVG-SPGEYKASVRRPEGVNVVVEPRVLKFDQVGEEKSFTVTLSGA-- 852
+++ R + NVG S Y+ +R P G V VEP L F +VG++ SF V +
Sbjct: 703 SKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEV 762
Query: 853 --SP-LNAVVDGILIWSDGKHFVSSPIVVS 858
SP V G ++WSDGK V+SP+VV+
Sbjct: 763 KLSPGATNVETGHIVWSDGKRNVTSPLVVT 770
BLAST of Lag0025721 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 505.0 bits (1299), Expect = 1.2e-142
Identity = 305/773 (39.46%), Postives = 437/773 (56.53%), Query Frame = 0
Query: 106 KKSYVVLLGSHSHGLEVSEADLQRVTNSHYKLLGSFLGSNKKAKDAIFYSYKKHINGFAA 165
KK+Y++ V+ +D +H+ S L S + ++ Y+Y +GF+A
Sbjct: 27 KKTYII---------RVNHSDKPESFLTHHDWYTSQLNS----ESSLLYTYTTSFHGFSA 86
Query: 166 MLEEEEA-AELAKHREVAVVLGNEMMELHTTHSVGEWTISYHCGDLCRVLVPYTFMALHA 225
L+ EA + L+ + + + + LHTT + ++ G +H
Sbjct: 87 YLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG-------------VHD 146
Query: 226 FGEE----CFSNLDSGVWPESMSFKDDGINKPIPSKWKGSCENPHN-NGFHCNRKLIGAK 285
G LD+GVWPES SF DD IPSKWKG CE+ + + CN+KLIGA+
Sbjct: 147 LGSSSNGVIIGVLDTGVWPESRSF-DDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 206
Query: 286 FFNEGYIAYMRSINANFDVSSVINSTHDYAGHGSHTLSTAGGNFVAPKDVFGSSGFGSAK 345
F++G + + F S D GHG+HT +TA G+ V G + G+A+
Sbjct: 207 SFSKG---FQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAA-GTAR 266
Query: 346 GGSPRARVAAYKVCWGDNVGCPTADILQGVDEAIHDGVDVISYSVGGGPSPFYQDGFAIA 405
G + RARVA YKVCW + GC +DIL +D AI DGVDV+S S+GGG +P+Y+D AI
Sbjct: 267 GMATRARVATYKVCW--STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIG 326
Query: 406 SFHAVKNGISVVCSAGNSGPDPGSAVNLAPWMLTVGASTLDRRIASVVQLNNGQSFQGES 465
+F A++ G+ V CSAGNSGP S N+APW++TVGA TLDR + L NG+ G S
Sbjct: 327 AFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVS 386
Query: 466 LSKGL-----PEEKYYPLISGGQAAAAGAYSGDAMLCKPGTLDPAKVKGKILACLRGETA 525
L G+ P E Y + G ++++ LC PG+LD + V+GKI+ C RG A
Sbjct: 387 LYSGVGMGTKPLELVY---NKGNSSSSN-------LCLPGSLDSSIVRGKIVVCDRGVNA 446
Query: 526 RIDKGIQAENAGAVGMVLCNDEGNGEQILADFHVLPAIQIGYEDGLAVFNYIDNTQNPSA 585
R++KG +AG +GM++ N +GE+++AD H+LPAI +G + G + Y+ + P+A
Sbjct: 447 RVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTA 506
Query: 586 YIVPPTEKFDVTPAPIMAKFSSRGPNLITPEIIKPDITAPGVNIIAATTQAHSPSDDPSH 645
+V DV P+P++A FSSRGPN +TPEI+KPD+ PGVNI+A + A P+
Sbjct: 507 LLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKD 566
Query: 646 NRKTPYVSMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTSARVRDNTMHPMQDGG 705
+R+T + MSGTSMSCPHISG+ GLLK HP+WSP+AIKSA+MT+A V DNT P+ D
Sbjct: 567 SRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAA 626
Query: 706 SERGELATPFSYGSGHVQPVGAMDPGLVYDLTTEDYLDFLCALGYD-ESMMRVFSDEAYQ 765
L+ P+++GSGHV P A+ PGLVYD++TE+Y+ FLC+L Y + ++ + +
Sbjct: 627 D--NSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN 686
Query: 766 CPPSAS-LLNFNYPSIAVQNLNNGRVTVTRKLKNVGSPGE-YKASVRRPEGVNVVVEPRV 825
C S NYPS +V V TR++ NVG+ YK +V V + V+P
Sbjct: 687 CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 746
Query: 826 LKFDQVGEEKSFTVTL---SGASPLNAVVDGILIWSDGKHFVSSPIVVSSGPF 862
L F VGE+K +TVT G S N G + WS+ +H V SP+ S F
Sbjct: 747 LSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 6.1e-232 | 56.68 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 3.1e-212 | 52.15 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 3.3e-169 | 44.61 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 4.4e-158 | 45.80 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9LUM3 | 5.8e-142 | 40.80 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CLZ9 | 0.0e+00 | 77.17 | subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012693 P... | [more] |
| A0A6J1CN43 | 6.2e-288 | 65.27 | subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P... | [more] |
| A0A5D3CZ66 | 5.5e-268 | 62.57 | Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A0A0LVY8 | 1.3e-266 | 62.22 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1 | [more] |
| A0A1S3CLB7 | 2.3e-266 | 61.96 | subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=... | [more] |
