Lag0025536 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0025536
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NADH-ubiquinone oxidoreductase
Location	chr10: 14737688 .. 14738216 (-)
RNA-Seq Expression	Lag0025536
Synteny	Lag0025536

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCGTATCGTCTTCAACCGCAGCGAGTTGGCAAAGCGAGTTTTTGCAGAGTCACACAGCGATACAGAGAGAAAGAGAGCCGGCGGAGATCAGTTTCGGTTTCAGCTCCGGAGAAGACAAGATGAACACCGACATTACTGCGTGGACCAAGCCAAAGTACCCAGTAATAGATCGGAATCCTTCATTCACCAAAGTCGTCGGCAATTTCGACCCTATCGATTACTTCTTTATCTTCAACTTCACCGGCGTTTCCGTCTGGATCTCTCGTGACGGTATCAATCTCTTCTTCCTTCTCTCCTTCCTTCTTCCTCTTCCATTTCGTTGCTAATCTAGAAATGTGTTCTCTTCTTTTCGCTTTAGAGATTAAGCGCGGGATTGGAAAAATGCACAATATTAGGGGCCCCTTGATGGTGACCGGTGGTCTAATTGGGCTGATGGGAGGCTTCATCTTCGCTTATAAGGACTCGGCGGCTCGGCTTAAGGGGTTTTACCCCAATGAACGCGAGGTAGTTCGTTACAAGAAATAA

mRNA sequence

ATGCTCGTATCGTCTTCAACCGCAGCGAGTTGGCAAAGCGAGTTTTTGCAGAGTCACACAGCGATACAGAGAGAAAGAGAGCCGGCGGAGATCAGTTTCGGTTTCAGCTCCGGAGAAGACAAGATGAACACCGACATTACTGCGTGGACCAAGCCAAAGTACCCAGTAATAGATCGGAATCCTTCATTCACCAAAGTCGTCGGCAATTTCGACCCTATCGATTACTTCTTTATCTTCAACTTCACCGGCGTTTCCGTCTGGATCTCTCGTGACGAGATTAAGCGCGGGATTGGAAAAATGCACAATATTAGGGGCCCCTTGATGGTGACCGGTGGTCTAATTGGGCTGATGGGAGGCTTCATCTTCGCTTATAAGGACTCGGCGGCTCGGCTTAAGGGGTTTTACCCCAATGAACGCGAGGTAGTTCGTTACAAGAAATAA

Coding sequence (CDS)

ATGCTCGTATCGTCTTCAACCGCAGCGAGTTGGCAAAGCGAGTTTTTGCAGAGTCACACAGCGATACAGAGAGAAAGAGAGCCGGCGGAGATCAGTTTCGGTTTCAGCTCCGGAGAAGACAAGATGAACACCGACATTACTGCGTGGACCAAGCCAAAGTACCCAGTAATAGATCGGAATCCTTCATTCACCAAAGTCGTCGGCAATTTCGACCCTATCGATTACTTCTTTATCTTCAACTTCACCGGCGTTTCCGTCTGGATCTCTCGTGACGAGATTAAGCGCGGGATTGGAAAAATGCACAATATTAGGGGCCCCTTGATGGTGACCGGTGGTCTAATTGGGCTGATGGGAGGCTTCATCTTCGCTTATAAGGACTCGGCGGCTCGGCTTAAGGGGTTTTACCCCAATGAACGCGAGGTAGTTCGTTACAAGAAATAA

Protein sequence

MLVSSSTAASWQSEFLQSHTAIQREREPAEISFGFSSGEDKMNTDITAWTKPKYPVIDRNPSFTKVVGNFDPIDYFFIFNFTGVSVWISRDEIKRGIGKMHNIRGPLMVTGGLIGLMGGFIFAYKDSAARLKGFYPNEREVVRYKK

Homology

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BLAST of Lag0025536 vs. NCBI nr
Match: KAG6583741.1 (Heterogeneous nuclear ribonucleoprotein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 150.2 bits (378), Expect = 1.4e-32
Identity = 83/137 (60.58%), Postives = 99/137 (72.26%), Query Frame = 0


Query: 7   TAASWQSEFLQSHTAIQREREPAEISFGFSSGEDKMNTDITAWTKPKYPVIDRNPSFTKV 66
           TAAS Q+ +   +   QR REPAE+S  FS+GED MNTDITA TKP+YPVIDRNP FTKV
Sbjct: 136 TAASRQANY---YRVAQRGREPAEVS--FSTGEDVMNTDITASTKPEYPVIDRNPPFTKV 195

Query: 67  VGNFDPIDYFFIFNFTGVSVWIS-RDEIKRGIGKMHNIRGPLMVTGGLIGLMGGFIFAYK 126
           VGNFD +DY      TGVSV +     IK G      IRGP MVTGGLIGLMGGF++AY+
Sbjct: 196 VGNFDTLDYLRFVTITGVSVTVGYLSGIKPG------IRGPSMVTGGLIGLMGGFMYAYQ 255

Query: 127 DSAARLKGFYPNEREVV 143
           +SA R+ GF+PNE E++
Sbjct: 256 NSAGRIMGFFPNEGEIL 261

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BLAST of Lag0025536 vs. NCBI nr
Match: XP_022142552.1 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Momordica charantia] >XP_022925305.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita moschata] >XP_022966760.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita maxima] >XP_023001508.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita maxima] >XP_023541604.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita pepo subsp. pepo] >KAG6573532.1 hypothetical protein SDJN03_27419, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012647.1 hypothetical protein SDJN02_25399 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 136.0 bits (341), Expect = 2.7e-28
Identity = 71/106 (66.98%), Postives = 79/106 (74.53%), Query Frame = 0


Query: 42  MNTDITAWTKPKYPVIDRNPSFTKVVGNFDPIDYFFIFNFTGVSVWIS-RDEIKRGIGKM 101
           MNTDITA TKP+YPVIDRNP FTKVVGNFD +DY      TGVSV +     IK G    
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPG---- 60

Query: 102 HNIRGPLMVTGGLIGLMGGFIFAYKDSAARLKGFYPNEREVVRYKK 147
             IRGP MVTGGLIGLMGGF++AY++SA RL GF+PNE EV RYKK
Sbjct: 61  --IRGPSMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEGEVARYKK 100

[Close]

BLAST of Lag0025536 vs. NCBI nr
Match: XP_022927362.1 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita moschata] >XP_023520383.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 135.2 bits (339), Expect = 4.6e-28
Identity = 70/106 (66.04%), Postives = 79/106 (74.53%), Query Frame = 0


Query: 42  MNTDITAWTKPKYPVIDRNPSFTKVVGNFDPIDYFFIFNFTGVSVWIS-RDEIKRGIGKM 101
           MNTDITA TKP+YPVIDRNP FTKVVGNFD +DY      TGVSV +     IK G    
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPG---- 60

Query: 102 HNIRGPLMVTGGLIGLMGGFIFAYKDSAARLKGFYPNEREVVRYKK 147
             IRGP MVTGGLIGLMGGF++AY++SA R+ GF+PNE EV RYKK
Sbjct: 61  --IRGPSMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEGEVARYKK 100

[Close]

BLAST of Lag0025536 vs. NCBI nr
Match: KAG7019380.1 (hypothetical protein SDJN02_18341 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 135.2 bits (339), Expect = 4.6e-28
Identity = 70/106 (66.04%), Postives = 79/106 (74.53%), Query Frame = 0


Query: 42  MNTDITAWTKPKYPVIDRNPSFTKVVGNFDPIDYFFIFNFTGVSVWIS-RDEIKRGIGKM 101
           MNTDITA TKP+YPVIDRNP FTKVVGNFD +DY      TGVSV +     IK G    
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPG---- 60

Query: 102 HNIRGPLMVTGGLIGLMGGFIFAYKDSAARLKGFYPNEREVVRYKK 147
             IRGP MVTGGLIGLMGGF++AY++SA R+ GF+PNE EV RYKK
Sbjct: 61  --IRGPSMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 100

[Close]

BLAST of Lag0025536 vs. NCBI nr
Match: XP_008463609.1 (PREDICTED: NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Cucumis melo] >XP_038894012.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Benincasa hispida])

HSP 1 Score: 134.8 bits (338), Expect = 6.0e-28
Identity = 70/106 (66.04%), Postives = 79/106 (74.53%), Query Frame = 0


Query: 42  MNTDITAWTKPKYPVIDRNPSFTKVVGNFDPIDYFFIFNFTGVSVWIS-RDEIKRGIGKM 101
           MNTDITA TKP+YPVIDRNP FTKVVGNFD +DY      TGVSV +     IK G    
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPG---- 60

Query: 102 HNIRGPLMVTGGLIGLMGGFIFAYKDSAARLKGFYPNEREVVRYKK 147
             IRGP MVTGGLIGLMGGF++AY++SA RL GF+PN+ EV RYKK
Sbjct: 61  --IRGPSMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVARYKK 100

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BLAST of Lag0025536 vs. ExPASy Swiss-Prot
Match: Q02854 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=nuo20.9 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.8e-04
Identity = 28/91 (30.77%), Postives = 43/91 (47.25%), Query Frame = 0


Query: 54  YPVIDRNPSFTKVVGNFDPIDYFF--IFNFTGVS-VWISRDEIKRGIGKMHNIRGPLMVT 113
           YP+ID +P F +V+G   P DY    +    G   +++       G+GK    +   + T
Sbjct: 19  YPLIDNDPHFRRVIGYARPSDYVHGTVAGAAGPGLLYLMEKMAPSGVGKGGFPKAMRLAT 78

Query: 114 GGLIGLMGGFIFAYKDSAARLKGFYPNEREV 142
              +G  GGF++ Y+ S  R  G   N REV
Sbjct: 79  A--VGFFGGFLYFYQRSILRFYGMSENAREV 107

The following BLAST results are available for this feature:

BLAST of Lag0025536 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6583741.1	1.4e-32	60.58	Heterogeneous nuclear ribonucleoprotein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022142552.1	2.7e-28	66.98	NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Momordica charantia] >XP_022925...	[more]
XP_022927362.1	4.6e-28	66.04	NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita moschata] >XP_02...	[more]
KAG7019380.1	4.6e-28	66.04	hypothetical protein SDJN02_18341 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_008463609.1	6.0e-28	66.04	PREDICTED: NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Cucumis melo] >XP_03...	[more]

BLAST of Lag0025536 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q02854	4.8e-04	30.77	NADH-ubiquinone oxidoreductase 20.9 kDa subunit OS=Neurospora crassa (strain ATC...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019721	NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal	PFAM	PF10785	NADH-u_ox-rdase	coord: 52..135 e-value: 1.7E-17 score: 63.9
None	No IPR available	PANTHER	PTHR34062	OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED	coord: 42..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0025536.1	Lag0025536.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005747	mitochondrial respiratory chain complex I