Lag0025345 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0025345
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING/U-box superfamily protein
Location	chr10: 11665279 .. 11669660 (-)
RNA-Seq Expression	Lag0025345
Synteny	Lag0025345

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCCTCGTGAGATTTTTGGTCGTGGGTCTGCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCACGAGGTATGGAAAGAGACCGAGGATGTAGTAGAAGAAGAGTCTGAGGAGGAGGAGGAGGAGGAGGAACTTGATGAGTTTTCAGTAAAAATGTTCTTTAAGGGCGTTTCAATAGCTGAGATTGGTGACCCTGCTTCGGGTCTTTCTGGTATTGGAGTGTTCATGGAAAAATCAGGGAAAGTCTCTTTTATTCAGGTCCAGAAAAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGACATTCGCTGTATTCGAGCTTTCACTGACTCCGATCTGTTATATAATCAGGTTATTATTTCCCCTATCTGCCTAAACCTTGCGTGTTTTGATTTATGCCAATTATCATTTTTATTTCTATTGGAGAGTTTATCATACTCTTTTTTGAAAGTTTGTTGCTGTTTGTTGACTCTATTTTTGGGGTTCTTTTGCATTGTTTTCAAAAGAAAAATGGAGAATTGTTCTCACTCCTACTCTCAGTAATGTGGTTTTCTGGAAACTCCAGGCTTTTTAACATTTGACTAGTTGTATTTGATTTTCCTTATAATAAATGAATTTTTTGATGAAATATAATTTGTTGAGAAAGGGGAGGGGATAGAATTGATTATTACCATTTCTTCCGCACAAGTCAGAGGAGTATGGGGTTGGTTACATGTTTCACGGCCAGTAATTTGAGTGTTGGGACTTTATGAGCTGTGGAAGTCGTGGAGAGTGGAGAGATGGATAAACTCTTACAGTTTTCTAGCCATGCGCTTACAAGTTTTTCTTATATGCTTAAGATAATGTGAATGTTTGACAGATAACAAATGTAGAAGAGCCTGAAATTCCACTTCTTGTAGCGCTTAAAGAAAGGATTCTAGAACATGCTAGCACTTTTGAAGCCTTTTCCCTGAATCTTTGTTCAAGTACTGATCTTGAGCAGCCATTTCATCTTGCCAAAGTGGCCATTGGGGTTCTTTCTTGTCCGCAAAAGGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGAGAACAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCCATCAGATGCCCTCAGTTGAGATGCAAATATTACATCAAAAGCAATGAGTTTAAATCTTTCCTTCCACTGACTTTATATGAATTGTTAGAGAGTACCTTCGCTGAAGCAAATATTCAATCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCCACCGAATGTTTGTCAGCTCGAGCAAGTTCATCAAGTCAATCAGATAATAGCTGTATTGAGTGCCCAGTTTGTCAGAGATTTATCTGTGTTGAATGTCGTGTTCCTTGGCACTCATCCATGAGTTGTGAAGAGTTTCAGAACATTCCATTAGATGAGAGAGACACTGAAGATATTACCTTGCATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGCAGTGTCGTAGAATGATTGAATTGTCTCAAGGTTGCTACCATATGACTTGCTGGTAAGGCTACTTCCTTCATATCTCTTTATTTCTATTAACCTGTTTGTTTATGAGATATTGTGGAAGCACCATCATCAGAAACATATATCACAAGTTTAGTTGGATGTTAGATTTATAAGGTTTTGGACCTTTGGTTGGAGGACGATTTGAAAATTTGGTGCACTTTATATGCTTATGTAGTTATGTGGATAATCAACAGGCATGGCATACAAATAGAATCTTCCCTCCCCATTTTAATGTCTTTGTTTTCTGCTAGATTAGATCTTTTATTGTTTTACAAATTTTCTAGCGACCATCATTTACTATTTAGTCTATCTCCCTGAGGAACTTCTGTTTATCCCAAACTCTACTGGTTTACTAATCTCATGGGCTACCTAAATTCAGGCTGCTAGAATTCACCTATTAGGCTATTTACTTACTTATCACAAATCCACAGACTAATTACATGTTGGAACTTGGAACTCATTTCTCTAGTCTACGCAATTCAAACACATACTACATAAACCTAAACTTCTCCCCTCAAGTTTACCAAACCCAAGTTTATTGGAATTGCTAGCCAAACATTCCCGTAGATTTTTATGTCAATACAGTGCCTATACCGTAGGGAAAATCTTAATTTGGTGGTTTATATGTTATAACAGCCTTATTGTTCAATGTGATGAACATAATTGATGTATACATCACAAGTAGGTCTAACTTATTTGAAGAAATGAAATGTGGGGAAACACATGATAGTGCTAAAATATCATTTGCCATTATGTATTGTGCAGTCTAAGTTGGTCATACAATGATTACAGCCTTTCTAGGGATTTCTCTAGAGATGAGGTCAGAGGTTCGAATCCCCCCATCCCCAAATGTTGTTGTACTCAAAAAAAAAAAAAAAAATATTAACGGTTGCTGTGGTAAAAGAGACGAAATATTAGCTCAACTTTAAATGTTTCTACTTGTCTTCAGAGTTCCTCCATCTGCCGTTTTTGTGAGAAGAGGAGGTTTTTGTGGCAAGCTAGGATGTGTGCTCTGTTATTGAGTCTATGGGGGAGAGAAATAATAGAGTGTTTAGGGGGATAGAGGTCTGTGTGATATGTGGTCCCTTTCGAGGTTCCATGTATCCCGATGGCCATCATCAGTTACGAAATTATTGTGTAATTACTCATTAGGTCTCATTTTACTTTATTGGAGTCTCTTCCTTCAATTGGACTCCTTTTGGTAGGCTTGTTTTTTGTATGTCCTTGTATTCTTTCATTTTTTTTGCAATGAAAGTTGGCTTATTATAAAAAAAAGTTCGCTTGTCTTGTAATTTTTTATATCTTTTGGGTGTTGAACATGATGAGCGGGATTTTCTTATTTTTGCTTTTTCTACTCTTAACAGTTTCTGGCTGTTTCCTTTTAATTTCACATTCAAACTCTTATCCAAGAGTCTGTCAAGCACGCTACTATTAGATCAGCCAACTGTTTGTGCACACTGTATTCTTGTTGTCACTCACACATGGCTAAATGTCAGTCAACGTAGTATTGAGTCATATCTTATTCCTTCCTCATTACTGGACTTTGCGTACAAGTTGATTGTTGTGTGAATTATAATAGCTTATCGGTGAATAATATTACATATGTACCCCAGCACCCCCCCCCCCCCAAATGAGGGGCTGGATTCGAGTCTTTTACATGTGTAATCATTTCAAGAAATACCTACCGCAGATGCATTTCTCCTGCTCATTAGTTGCATATTCAATCTGCCCATGAAACTCTCATATCTCACACTACCAATCTAGAGCCGTCTTTTTTATTTTGTTGCACACAGTTCTCCTAGTTGGTTCTGAAGTTTAGATCCCAACACTTGGATGATGAATCCAAGGTCTTTTATTCCAAAACCATTGATTTACATTCTTTGTCCATTTAGTGAATATAATTCCTGTCTGGGTGTTTGCCGAATCGAGATCTTCTCTTACTACCAAGCTAGAGCTGTCTTGTTGATTTTATTGCACACATTTCTCCTAGTAGGTTCTAAAGTTTAGAACCCAACACTTGATTGGATGATGAATCCGACGTCTTTTATTCTAAACCCATTGTTTTACATTCTTTGTTCATTTAGTGGCTATTATTCCTGTGTGGGTGTTTGGTGAATTGAGATCTTTGAACGTGCACTAGCACTACTGTCACATACATTTGTGGTCAAGCGAATGAGGTTCAATTTTAGCAAAACATGATCTGAAAATTCCAAATTGCAATTCTTGGTTTTTTACTAAACTAGTTAGTGCAGGGCTTTATGTATCTGACTTTAATGTTTATACTGCTTTGCTTAATATGTTTACAGGTGCGGACACGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGATGACAATTCCGAGGACTTGGTCACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTTCCCATGATAATGGATGCATACTCAGAACAAGAGAGGTCACAATTAGCATTAATTCAACGATTCCTCGCGGGGGGATTTAGTCTGAGCGACCACCAACCATATCAATCTCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTGTGATAAGCGATAGCTACTACGAAGATTATGTACAATGA

mRNA sequence

ATGGAGCCTCGTGAGATTTTTGGTCGTGGGTCTGCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCACGAGGTATGGAAAGAGACCGAGGATGTAGTAGAAGAAGAGTCTGAGGAGGAGGAGGAGGAGGAGGAACTTGATGAGTTTTCAGTAAAAATGTTCTTTAAGGGCGTTTCAATAGCTGAGATTGGTGACCCTGCTTCGGGTCTTTCTGGTATTGGAGTGTTCATGGAAAAATCAGGGAAAGTCTCTTTTATTCAGGTCCAGAAAAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGACATTCGCTGTATTCGAGCTTTCACTGACTCCGATCTGTTATATAATCAGATAACAAATGTAGAAGAGCCTGAAATTCCACTTCTTGTAGCGCTTAAAGAAAGGATTCTAGAACATGCTAGCACTTTTGAAGCCTTTTCCCTGAATCTTTGTTCAAGTACTGATCTTGAGCAGCCATTTCATCTTGCCAAAGTGGCCATTGGGGTTCTTTCTTGTCCGCAAAAGGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGAGAACAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCCATCAGATGCCCTCAGTTGAGATGCAAATATTACATCAAAAGCAATGAGTTTAAATCTTTCCTTCCACTGACTTTATATGAATTGTTAGAGAGTACCTTCGCTGAAGCAAATATTCAATCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCCACCGAATGTTTGTCAGCTCGAGCAAGTTCATCAAGTCAATCAGATAATAGCTGTATTGAGTGCCCAGTTTGTCAGAGATTTATCTGTGTTGAATGTCGTGTTCCTTGGCACTCATCCATGAGTTGTGAAGAGTTTCAGAACATTCCATTAGATGAGAGAGACACTGAAGATATTACCTTGCATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGCAGTGTCGTAGAATGATTGAATTGTCTCAAGGTTGCTACCATATGACTTGCTGGTGCGGACACGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGATGACAATTCCGAGGACTTGGTCACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTTCCCATGATAATGGATGCATACTCAGAACAAGAGAGGTCACAATTAGCATTAATTCAACGATTCCTCGCGGGGGGATTTAGTCTGAGCGACCACCAACCATATCAATCTCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTGTGATAAGCGATAGCTACTACGAAGATTATGTACAATGA

Coding sequence (CDS)

ATGGAGCCTCGTGAGATTTTTGGTCGTGGGTCTGCTCATGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCACGAGGTATGGAAAGAGACCGAGGATGTAGTAGAAGAAGAGTCTGAGGAGGAGGAGGAGGAGGAGGAACTTGATGAGTTTTCAGTAAAAATGTTCTTTAAGGGCGTTTCAATAGCTGAGATTGGTGACCCTGCTTCGGGTCTTTCTGGTATTGGAGTGTTCATGGAAAAATCAGGGAAAGTCTCTTTTATTCAGGTCCAGAAAAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTGGAGGCCATGCAGAATGACATTCGCTGTATTCGAGCTTTCACTGACTCCGATCTGTTATATAATCAGATAACAAATGTAGAAGAGCCTGAAATTCCACTTCTTGTAGCGCTTAAAGAAAGGATTCTAGAACATGCTAGCACTTTTGAAGCCTTTTCCCTGAATCTTTGTTCAAGTACTGATCTTGAGCAGCCATTTCATCTTGCCAAAGTGGCCATTGGGGTTCTTTCTTGTCCGCAAAAGGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGAGAACAAACCATCTGCTATGATGGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCCATCAGATGCCCTCAGTTGAGATGCAAATATTACATCAAAAGCAATGAGTTTAAATCTTTCCTTCCACTGACTTTATATGAATTGTTAGAGAGTACCTTCGCTGAAGCAAATATTCAATCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCCACCGAATGTTTGTCAGCTCGAGCAAGTTCATCAAGTCAATCAGATAATAGCTGTATTGAGTGCCCAGTTTGTCAGAGATTTATCTGTGTTGAATGTCGTGTTCCTTGGCACTCATCCATGAGTTGTGAAGAGTTTCAGAACATTCCATTAGATGAGAGAGACACTGAAGATATTACCTTGCATCGTCTTGCACAAAACAAAAGGTGGAGGCGTTGTCAGCAGTGTCGTAGAATGATTGAATTGTCTCAAGGTTGCTACCATATGACTTGCTGGTGCGGACACGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGATGACAATTCCGAGGACTTGGTCACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTTCCCATGATAATGGATGCATACTCAGAACAAGAGAGGTCACAATTAGCATTAATTCAACGATTCCTCGCGGGGGGATTTAGTCTGAGCGACCACCAACCATATCAATCTCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTGTGATAAGCGATAGCTACTACGAAGATTATGTACAATGA

Protein sequence

MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVKMFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDLEQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ

Homology

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BLAST of Lag0025345 vs. NCBI nr
Match: XP_008459101.1 (PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >XP_008459102.1 PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >KAA0043320.1 E3 ubiquitin-protein ligase arih1 [Cucumis melo var. makuwa] >TYK29385.1 E3 ubiquitin-protein ligase arih1 [Cucumis melo var. makuwa])

HSP 1 Score: 990.3 bits (2559), Expect = 6.1e-285
Identity = 472/520 (90.77%), Postives = 498/520 (95.77%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME R+IFGRGS+HGPAEEDDVE+FRSCCEDHEVWKETED VEEES     EE+LDEFSVK
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES-----EEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKGVSIAEIGDPASGLSGIGVFMEKSGK+ F+QVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQND+RCIRAFTDSDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL L SS DL
Sbjct: 121 AMQNDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS C+K+YVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           ++ SQVPIRCPQLRCKYYI SNEFKSFLPLTLYELLE+T AEANI +DRIYCPFPNCSVL
Sbjct: 241 VDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQSDNSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDT D
Sbjct: 301 LDPSECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD
Sbjct: 361 ITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Lag0025345 vs. NCBI nr
Match: XP_022142466.1 (uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142467.1 uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142468.1 uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142469.1 uncharacterized protein LOC111012584 [Momordica charantia])

HSP 1 Score: 980.7 bits (2534), Expect = 4.9e-282
Identity = 476/520 (91.54%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKE ED+VEE S   EEEE LDEFSVK
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGS---EEEEHLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKG+SIAEIGDPASGLSGIGVFMEKSGKV FIQVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQN+IRCIRAFTDSDLLYNQITN EE EIPLL+ALKERILEHASTFEAFSLNL SSTDL
Sbjct: 121 AMQNEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMV LKCCHKFCSHCMK+YVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           +++SQVPIRCPQLRCKYYI  NEF+SFLPLTLYE LE+T AEANI SDRIYCPFPNCSVL
Sbjct: 241 VQSSQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLS RASSSSQSDNSCIECPVCQRFICV C VPWHSSMSCEEFQNI     DTED
Sbjct: 301 LDPSECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTED 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRW+RCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQD QQTCQCA WD
Sbjct: 361 ITLHRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           ++NSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP+
Sbjct: 421 EENSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPH 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

[Close]

BLAST of Lag0025345 vs. NCBI nr
Match: XP_004145453.1 (E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >XP_011660321.1 E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >KGN66894.1 hypothetical protein Csa_007170 [Cucumis sativus])

HSP 1 Score: 977.6 bits (2526), Expect = 4.1e-281
Identity = 467/520 (89.81%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKETED VEEES     EE+LDEFSVK
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEES-----EEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKGVSIAEIGD ASGLSGIGV MEKSGK+ F+QVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGVSIAEIGDSASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQND+RCIRAFT SDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL+L S  DL
Sbjct: 121 AMQNDVRCIRAFTSSDLLYKQITNHEEPEIPLLVALKERILEHASNFESFSLSLSSGADL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           +QPFHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS CMK+YVDGK
Sbjct: 181 QQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           ++ SQVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE+T AEANI +DRIYCPFPNCSVL
Sbjct: 241 VDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQS+NSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDT D
Sbjct: 301 LDPSECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD
Sbjct: 361 ITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPC 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Lag0025345 vs. NCBI nr
Match: XP_038894440.1 (uncharacterized protein LOC120083025 [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 462/512 (90.23%), Postives = 489/512 (95.51%), Query Frame = 0


Query: 9   RGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVKMFFKGVSI 68
           RGS+HGPAEED+VEDFRSCCEDHEVWKETE+ VEEES      E+LDEFSVKMFFKGVSI
Sbjct: 5   RGSSHGPAEEDEVEDFRSCCEDHEVWKETEEAVEEES------EDLDEFSVKMFFKGVSI 64

Query: 69  AEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRC 128
           AEIGDPASGLSGIGVFMEKSGK+ F+QVQKKLDFYVDELVADYLALMDGLVEAMQND+ C
Sbjct: 65  AEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQNDVCC 124

Query: 129 IRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDLEQPFHLAK 188
           IRAFTDSDLLY QITN E+PEIPLLVALKERILEHAS FEAFSLNL SSTDLEQP HLAK
Sbjct: 125 IRAFTDSDLLYKQITNEEDPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQPLHLAK 184

Query: 189 VAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQVPI 248
           VAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCSHCMK+YVDGK++ SQVPI
Sbjct: 185 VAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSHCMKTYVDGKVDASQVPI 244

Query: 249 RCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVLLDPTECLS 308
           RCPQLRCKYYI SNEFKSFLPLTLYE LE+T AEANI SDRIYCPFPNCSVLLDP+ECLS
Sbjct: 245 RCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDPSECLS 304

Query: 309 ARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQ 368
           ARASSSSQSDNSCIECPVCQRFICVEC+VPWHSSMSCEEFQ++PL+ERDT DITLHRLAQ
Sbjct: 305 ARASSSSQSDNSCIECPVCQRFICVECQVPWHSSMSCEEFQSVPLEERDTADITLHRLAQ 364

Query: 369 NKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSEDLV 428
           NKRWRRCQ+C RMIEL+ GCYHMTCWCGHEFCYSCG EYQDG+QTCQCAFWD+D+SE++V
Sbjct: 365 NKRWRRCQECHRMIELAHGCYHMTCWCGHEFCYSCGVEYQDGEQTCQCAFWDEDDSENVV 424

Query: 429 THSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 488
           THS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 425 THSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 484

Query: 489 SYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           SYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 485 SYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 510

[Close]

BLAST of Lag0025345 vs. NCBI nr
Match: KAG7012597.1 (putative E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 950.7 bits (2456), Expect = 5.4e-273
Identity = 469/525 (89.33%), Postives = 488/525 (92.95%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME RE+F      GPAEEDDVEDFRSCCEDH      EDVVEE S EEEEEE+LDEFSVK
Sbjct: 1   MENREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGS-EEEEEEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKGVSIAEIGDP+SGLSGIGVFME+SGKV FIQVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGVSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           A+QN +R IRAFTDS+ LYNQITN EEPEIPLLVALKERILEHASTFEAFSLNL S +DL
Sbjct: 121 AIQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGD SVENCSICCENKP+AMMVALKCCHKFCSHCMKSYVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           LETSQVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE+T AEANI SD+IYCP+PNCSVL
Sbjct: 241 LETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSSMSCEEFQ+IPL+ERD  D
Sbjct: 301 LDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW- 420
           ITLHRLA+NKRWRRCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQDGQQTCQC FW 
Sbjct: 361 ITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWD 420

Query: 421 ----DDDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLS 480
               DDDNSEDLVTHSIQESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLS
Sbjct: 421 DDDNDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLS 480

Query: 481 DHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           DHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 DHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

[Close]

BLAST of Lag0025345 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 1.6e-17
Identity = 63/220 (28.64%), Postives = 99/220 (45.00%), Query Frame = 0


Query: 207 CSICCENKPSAMMVALKCCHK-FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYIKSNEFK 266
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  +++  E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLE--ETT 295

Query: 267 SFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECP 326
               LT  + ++  +     + D    P P C       +       S S+S    I+CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYK-IQCP 355

Query: 327 VCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRMIELS 386
            CQ   C +C  PWH  ++C+E++      R       H     +  ++C +C+  I+ +
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 415

Query: 387 QGCYHMTC-WCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 425
           +GC HMTC  C   FCY CG  Y+      Q  F+ D  S
Sbjct: 416 EGCDHMTCSQCNTNFCYRCGERYR------QLRFFGDHTS 440

[Close]

BLAST of Lag0025345 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 91.7 bits (226), Expect = 2.7e-17
Identity = 63/220 (28.64%), Postives = 99/220 (45.00%), Query Frame = 0


Query: 207 CSICCENKPSAMMVALKCCHK-FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYIKSNEFK 266
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  +++  E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLE--ETT 322

Query: 267 SFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECP 326
               LT  + ++  +     + D    P P C       +       S S+S    I+CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 327 VCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRMIELS 386
            CQ   C +C  PWH  ++C+E++      R       H     +  ++C +C+  I+ +
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 442

Query: 387 QGCYHMTC-WCGHEFCYSCGAEYQDGQQTCQCAFWDDDNS 425
           +GC HMTC  C   FCY CG  Y+      Q  F+ D  S
Sbjct: 443 EGCDHMTCSQCNTNFCYRCGERYR------QLRFFGDHTS 467

[Close]

BLAST of Lag0025345 vs. ExPASy Swiss-Prot
Match: Q9SKC4 (Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=ARI10 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.1e-15
Identity = 61/233 (26.18%), Postives = 93/233 (39.91%), Query Frame = 0


Query: 191 IGVLSCPQKGDKSVE---NCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQ-- 250
           +G+L  P       E    C IC E+     + ++ C H +C  C   Y+  K+E     
Sbjct: 102 VGILKEPVVDVNGTEVDIQCGICFESYTRKEIASVSCGHPYCKTCWTGYITTKIEDGPGC 161

Query: 251 VPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDR--IYCPFPNCSVLLDP 310
           + ++CP+  C   +  +           +     F  + ++  +   +CP P C      
Sbjct: 162 LRVKCPEPSCYAVVGQDMIDEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGC------ 221

Query: 311 TECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITL 370
            EC +     SS  D +C+    C    C  C    HS + CE         +D      
Sbjct: 222 -EC-AVEFGESSGYDVACL----CSYRFCWNCSEDAHSPVDCETVSKWIFKNQDE----- 281

Query: 371 HRLAQNKRW-----RRCQQCRRMIELSQGCYHMTC--WCGHEFCYSCGAEYQD 410
              ++NK W     + C +C+R IE S GC HMTC   CGH FC+ CG  Y D
Sbjct: 282 ---SENKNWILANSKPCPKCKRPIEKSHGCNHMTCSASCGHRFCWICGKSYSD 314

[Close]

BLAST of Lag0025345 vs. ExPASy Swiss-Prot
Match: Q9SKC3 (Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI9 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.5e-15
Identity = 55/219 (25.11%), Postives = 88/219 (40.18%), Query Frame = 0


Query: 207 CSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQ--VPIRCPQLRCKYYIKSNEF 266
           C IC E+     +  + C H +C  C   Y+  K+E     + ++CP+  C   +  +  
Sbjct: 128 CGICFESYTREEIARVSCGHPYCKTCWAGYITTKIEDGPGCLRVKCPEPSCSAAVGKDMI 187

Query: 267 KSFLPLTLYELLESTFAEANIQSDR--IYCPFPNCSVLLDPTECLSARASSSSQSDNSCI 326
           +      + E        + ++  +   +CP P C   ++         S SS  D SC+
Sbjct: 188 EDVTETKVNEKYSRYILRSYVEDGKKIKWCPSPGCGYAVE------FGGSESSSYDVSCL 247

Query: 327 ECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRW-----RRCQQ 386
               C    C  C    HS + C+         +D         ++NK W     + C +
Sbjct: 248 ----CSYRFCWNCSEDAHSPVDCDTVSKWIFKNQDE--------SENKNWMLANSKPCPE 307

Query: 387 CRRMIELSQGCYHMTCW--CGHEFCYSCGAEYQDGQQTC 415
           C+R IE + GC HMTC   CGHEFC+ C   Y+     C
Sbjct: 308 CKRPIEKNDGCNHMTCSAPCGHEFCWICLKAYRRHSGAC 328

[Close]

BLAST of Lag0025345 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 9.6e-15
Identity = 69/284 (24.30%), Postives = 110/284 (38.73%), Query Frame = 0


Query: 206 NCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQ--VPIRCPQLRCKYYIKSNE 265
           +C IC E+ P     AL C H++C  C K+Y++ K+      +   CP  +CK  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 266 FKSFLPLTLYELLESTFAEANIQSDR--IYCPFPNCSVLLDPTECLSARASSSSQSDNSC 325
           FK  +   ++E   +   ++ +  +    +CP P C          S R     + +   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 326 IECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRM 385
            +C     F C +  +  H    C +         D  +     LA  K   +C +CR  
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 386 IELSQGCYHMTC-----WCGHEFCYSC-----------GAEYQDGQQTCQCAFWDDDNSE 445
           IE + GC HMTC      CG EFC+ C           G  Y   +     A  DDD + 
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAH 377

Query: 446 DLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA 470
           D  T    E+  + +  + S    M    EQ R+     Q+ L+
Sbjct: 378 DAKTE--LEAYMFYYHRYESHRNAMKIADEQRRNAHLKEQQILS 408

[Close]

BLAST of Lag0025345 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.0e-285
Identity = 472/520 (90.77%), Postives = 498/520 (95.77%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME R+IFGRGS+HGPAEEDDVE+FRSCCEDHEVWKETED VEEES     EE+LDEFSVK
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES-----EEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKGVSIAEIGDPASGLSGIGVFMEKSGK+ F+QVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQND+RCIRAFTDSDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL L SS DL
Sbjct: 121 AMQNDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS C+K+YVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           ++ SQVPIRCPQLRCKYYI SNEFKSFLPLTLYELLE+T AEANI +DRIYCPFPNCSVL
Sbjct: 241 VDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQSDNSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDT D
Sbjct: 301 LDPSECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD
Sbjct: 361 ITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Lag0025345 vs. ExPASy TrEMBL
Match: A0A5D3E1X1 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001170 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.0e-285
Identity = 472/520 (90.77%), Postives = 498/520 (95.77%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME R+IFGRGS+HGPAEEDDVE+FRSCCEDHEVWKETED VEEES     EE+LDEFSVK
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES-----EEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKGVSIAEIGDPASGLSGIGVFMEKSGK+ F+QVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQND+RCIRAFTDSDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL L SS DL
Sbjct: 121 AMQNDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS C+K+YVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           ++ SQVPIRCPQLRCKYYI SNEFKSFLPLTLYELLE+T AEANI +DRIYCPFPNCSVL
Sbjct: 241 VDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQSDNSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDT D
Sbjct: 301 LDPSECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD
Sbjct: 361 ITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Lag0025345 vs. ExPASy TrEMBL
Match: A0A6J1CLL9 (uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012584 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.4e-282
Identity = 476/520 (91.54%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKE ED+VEE S   EEEE LDEFSVK
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGS---EEEEHLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKG+SIAEIGDPASGLSGIGVFMEKSGKV FIQVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQN+IRCIRAFTDSDLLYNQITN EE EIPLL+ALKERILEHASTFEAFSLNL SSTDL
Sbjct: 121 AMQNEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMV LKCCHKFCSHCMK+YVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           +++SQVPIRCPQLRCKYYI  NEF+SFLPLTLYE LE+T AEANI SDRIYCPFPNCSVL
Sbjct: 241 VQSSQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLS RASSSSQSDNSCIECPVCQRFICV C VPWHSSMSCEEFQNI     DTED
Sbjct: 301 LDPSECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTED 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRW+RCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQD QQTCQCA WD
Sbjct: 361 ITLHRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           ++NSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP+
Sbjct: 421 EENSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPH 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

[Close]

BLAST of Lag0025345 vs. ExPASy TrEMBL
Match: A0A0A0M0W5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G706610 PE=3 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 2.0e-281
Identity = 467/520 (89.81%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME REIFGRGS+HGPAEEDDVEDFRSCCEDHEVWKETED VEEES     EE+LDEFSVK
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKETEDAVEEES-----EEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKGVSIAEIGD ASGLSGIGV MEKSGK+ F+QVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGVSIAEIGDSASGLSGIGVLMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           AMQND+RCIRAFT SDLLY QITN EEPEIPLLVALKERILEHAS FE+FSL+L S  DL
Sbjct: 121 AMQNDVRCIRAFTSSDLLYKQITNHEEPEIPLLVALKERILEHASNFESFSLSLSSGADL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           +QPFHLAKVAIGVLSCPQKGDKSVENCSICC+NKPSAMMVALKCCHKFCS CMK+YVDGK
Sbjct: 181 QQPFHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCMKTYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           ++ SQVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE+T AEANI +DRIYCPFPNCSVL
Sbjct: 241 VDASQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHADRIYCPFPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQS+NSCIECPVCQRFICVEC+VPWHSS+SCEEFQNIPL+ERDT D
Sbjct: 301 LDPSECLSARASSSSQSENSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420
           ITLHRLAQNKRWRRCQ+C RMIEL+QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFWD
Sbjct: 361 ITLHRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWD 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPC 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISDSYYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Lag0025345 vs. ExPASy TrEMBL
Match: A0A6J1K3V2 (probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111490412 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.6e-273
Identity = 467/524 (89.12%), Postives = 488/524 (93.13%), Query Frame = 0


Query: 1   MEPREIFGRGSAHGPAEEDDVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVK 60
           ME RE+F      GPAEEDDVEDFRSCCEDH      EDVVEE S   EEEE+LDEFSVK
Sbjct: 1   MEHREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGS---EEEEDLDEFSVK 60

Query: 61  MFFKGVSIAEIGDPASGLSGIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVE 120
           MFFKG+SIAEIGDP+SGLSGIGVFME+SGKV FIQVQKKLDFYVDELVADYLALMDGLVE
Sbjct: 61  MFFKGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVE 120

Query: 121 AMQNDIRCIRAFTDSDLLYNQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDL 180
           A+QN +R IRAFTDS+ LYNQITN EEPEIPLLVALKERILEHASTFEAFSLNL S +DL
Sbjct: 121 AIQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGK 240
           EQPFHLAKVAIGVLSCPQKGDKS+ENCSICCENKP+AMMVALKCCHKFCSHCMKSYVDGK
Sbjct: 181 EQPFHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGK 240

Query: 241 LETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQSDRIYCPFPNCSVL 300
           LETSQVPIRCPQLRCKYYI SNEFKSFLPLTLYE LE+T AEANI SD+IYCP+PNCSVL
Sbjct: 241 LETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVL 300

Query: 301 LDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTED 360
           LDP+ECLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSSMSCEEFQ+IPL+ERD  D
Sbjct: 301 LDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIAD 360

Query: 361 ITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW- 420
           ITLHRLA+NKRWRRCQQCRRMIEL+QGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW 
Sbjct: 361 ITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD 420

Query: 421 ---DDDNSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSD 480
              DDDNSEDLVTHSIQESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSD
Sbjct: 421 DDDDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSD 480

Query: 481 HQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 521
           HQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 HQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

[Close]

BLAST of Lag0025345 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 660.2 bits (1702), Expect = 1.4e-189
Identity = 333/514 (64.79%), Postives = 408/514 (79.38%), Query Frame = 0


Query: 20  DVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVKMFFKGVSIAEIGDPASGLS 79
           D E+FRSCC D E+W +  D + +  EEE++++  DEFSVKM FKGVSI E GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAFTDSDLLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A TDS+LLY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 NQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDLEQPFHLAKVAIGVLSCPQK 199
           NQIT  E+ EIPLLVAL+ER+LE  S    F L L    DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYI 259
           GDK +ENCSICCE++ S MM++LKC HKFCSHCMK+YV+GK++TS+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 KSNEFKSFLPLTLYELLESTFAEANI---QSDRIYCPFPNCSVLLDPTECLS---ARASS 319
            + E KSFLP+T ++    +F EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFK----SFEEANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRW 379
           S+QS+NS C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P+DER  +DITLHRLA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSED---LVT 439
           RRCQQCR MIEL+QGC HMTC CGHEFCYSCGAEY++GQQTC CAFWDDD  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TD-SYVD-TMKDLHQLPWLERFVSVISDSYYEDY 519
           T+ SYV+  MKDLHQLPWLERFVSVISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

[Close]

BLAST of Lag0025345 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 660.2 bits (1702), Expect = 1.4e-189
Identity = 333/514 (64.79%), Postives = 408/514 (79.38%), Query Frame = 0


Query: 20  DVEDFRSCCEDHEVWKETEDVVEEESEEEEEEEELDEFSVKMFFKGVSIAEIGDPASGLS 79
           D E+FRSCC D E+W +  D + +  EEE++++  DEFSVKM FKGVSI E GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVSFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIRCIRAFTDSDLLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A TDS+LLY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 NQITNVEEPEIPLLVALKERILEHASTFEAFSLNLCSSTDLEQPFHLAKVAIGVLSCPQK 199
           NQIT  E+ EIPLLVAL+ER+LE  S    F L L    DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCENKPSAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYI 259
           GDK +ENCSICCE++ S MM++LKC HKFCSHCMK+YV+GK++TS+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 KSNEFKSFLPLTLYELLESTFAEANI---QSDRIYCPFPNCSVLLDPTECLS---ARASS 319
            + E KSFLP+T ++    +F EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFK----SFEEANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CIECPVCQRFICVECRVPWHSSMSCEEFQNIPLDERDTEDITLHRLAQNKRW 379
           S+QS+NS C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P+DER  +DITLHRLA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDNSED---LVT 439
           RRCQQCR MIEL+QGC HMTC CGHEFCYSCGAEY++GQQTC CAFWDDD  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TD-SYVD-TMKDLHQLPWLERFVSVISDSYYEDY 519
           T+ SYV+  MKDLHQLPWLERFVSVISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

[Close]

BLAST of Lag0025345 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 189.9 bits (481), Expect = 5.2e-48
Identity = 120/379 (31.66%), Postives = 200/379 (52.77%), Query Frame = 0


Query: 60  KMFFKGVSIAE--IGDPASGLSGIGV-FMEKSGKVSFIQVQKKLDFYVDELVADYLALMD 119
           +++FKG+   E   G     +SG GV   ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 120 GLVEAMQNDIRCIRAFTDSDLLYNQITN---VEEPEIPLLVALKERILEHASTFEAFSLN 179
           GL EA++  I+ I  F DS  ++  +T     ++ +I LL+   + I++H S+++     
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHV--- 265

Query: 180 LCSSTDLEQPFHLAKVAIGVLSCPQKGDKSV-----ENCSICCENKPSAMMVAL-KCCHK 239
           L +  D++  + LA+ +I  +  P +  +       E C+IC  +  +  M ++ KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 240 FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANIQ- 299
           FC  C+K +V+ KL     P +CP   CK  +  +     L   L +L +    E  I  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 300 SDRIYCPFPNCSVLLDPTECL-SARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSC 359
           ++R+YCP+P CS L+  T+   SA++  S    +    C  C+   CV+C+VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 360 EEFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGA 419
            E++ +   E   +D+ L  LA NK WR+C +C+ MIELSQGC H+TC CGHEFCY+CG 
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 420 EYQDGQQTC--QCAFWDDD 423
            +     TC  +C  W+++
Sbjct: 506 GWNKIMGTCLNRCPTWNEE 519

[Close]

BLAST of Lag0025345 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 4.9e-46
Identity = 112/308 (36.36%), Postives = 167/308 (54.22%), Query Frame = 0


Query: 112 LALMDGLVEAMQNDIRCIRAFTDSDLLYNQIT--NVEEPEIPLLVALKERILEHASTFEA 171
           +AL+ GL E+    IR +  + D  LLY  I      + ++  LV   + +LE  +  +A
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 172 FSLNLCSSTDLEQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMM--VALKCCHK 231
              +L +  D+   F LA+ AI       + D   E CSIC E      M     KC H+
Sbjct: 61  ---DLIALNDVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 232 FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANI-Q 291
            C  C+K YV+ KL +  VP  C    CK+ +        L L L E+ +    E +I  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 292 SDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMSCE 351
           ++RIYCP+PNCS+L+  TE  S    S   +D SC++C  C  F C++C+VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 352 EFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCGAE 411
           E++ +  D    +++ L  LA++K+WR+C+ CR MIELS  C HMTC CG++FCY C  E
Sbjct: 241 EYKKLHHDPL-VDELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQCEVE 292

Query: 412 YQDGQQTC 415
           +++ Q+TC
Sbjct: 301 WKNDQKTC 292

[Close]

BLAST of Lag0025345 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-43
Identity = 120/364 (32.97%), Postives = 187/364 (51.37%), Query Frame = 0


Query: 54  LDEFSVKMFFKG-VSIAEIGDPASGL----SGIGVFMEKSGKVSFIQVQKKLDFYVDELV 113
           +D    ++ FKG V+   + D          G+ +  EK   +  I+ +   D  +    
Sbjct: 149 MDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK-ESLRDIEISRRG 208

Query: 114 ADYLALMDGLVEAMQNDIRCIRAFTDSDLLYNQI--TNVEEPEIPLLVALKERILEHAST 173
            + +AL+ GL E+    +R +  + D D +Y  I      + +I  LV   + ILE  + 
Sbjct: 209 VEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVEEVQGILEKMAC 268

Query: 174 FEAFSLNLCSSTDLEQPFHLAKVAIGVLSCPQKGDKSVENCSICCENKP-SAMMVALKCC 233
            +A    L +  D++  F LA+ AIG  S     ++  E C I  E      M V  KC 
Sbjct: 269 IDAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETDVEHMFVTEKCL 328

Query: 234 HKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYIKSNEFKSFLPLTLYELLESTFAEANI 293
           H+ C  C+K +V  KL +   P  C +  CK+ +        L L L E+ +    E +I
Sbjct: 329 HRHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIEMWKQKMKEDSI 388

Query: 294 -QSDRIYCPFPNCSVLLDPTECLSARASSSSQSDNSCIECPVCQRFICVECRVPWHSSMS 353
             ++RIYCP+PNCS+L+  TE LS+ A  S+    +C++C  C  F C++C+VP H+ +S
Sbjct: 389 PAAERIYCPYPNCSMLMSKTE-LSSEADLSNV--RTCVKC--CGLF-CIDCKVPSHTDLS 448

Query: 354 CEEFQNIPLDERDTEDITLHRLAQNKRWRRCQQCRRMIELSQGCYHMTCWCGHEFCYSCG 409
            ++++ +  D    +D+ L  LA +K WR+C +CR MIELS GC HMTC CG+EFCY CG
Sbjct: 449 YDDYKKLHPDPL-VDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEFCYECG 499

The following BLAST results are available for this feature:

BLAST of Lag0025345 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459101.1	6.1e-285	90.77	PREDICTED: E3 ubiquitin-protein ligase arih1 [Cucumis melo] >XP_008459102.1 PRED...	[more]
XP_022142466.1	4.9e-282	91.54	uncharacterized protein LOC111012584 [Momordica charantia] >XP_022142467.1 uncha...	[more]
XP_004145453.1	4.1e-281	89.81	E3 ubiquitin-protein ligase arih1 [Cucumis sativus] >XP_011660321.1 E3 ubiquitin...	[more]
XP_038894440.1	1.3e-279	90.23	uncharacterized protein LOC120083025 [Benincasa hispida]	[more]
KAG7012597.1	5.4e-273	89.33	putative E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of Lag0025345 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D3YYI7	1.6e-17	28.64	Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P...	[more]
Q8TC41	2.7e-17	28.64	Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE...	[more]
Q9SKC4	1.1e-15	26.18	Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=AR...	[more]
Q9SKC3	2.5e-15	25.11	Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI...	[more]
Q6T486	9.6e-15	24.30	Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G...	[more]

BLAST of Lag0025345 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C9E4	3.0e-285	90.77	E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S...	[more]
A0A5D3E1X1	3.0e-285	90.77	E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1CLL9	2.4e-282	91.54	uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A0A0M0W5	2.0e-281	89.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G706610 PE=3 SV=1	[more]
A0A6J1K3V2	2.6e-273	89.12	probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Lag0025345 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19670.1	1.4e-189	64.79	RING/U-box superfamily protein	[more]
AT4G19670.2	1.4e-189	64.79	RING/U-box superfamily protein	[more]
AT5G60250.1	5.2e-48	31.66	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G25360.1	4.9e-46	36.36	RING/U-box superfamily protein	[more]
AT2G25370.1	1.3e-43	32.97	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 31..60
None	No IPR available	GENE3D	1.20.120.1750		coord: 343..469 e-value: 2.2E-15 score: 59.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR11685:SF353	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 18..519
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 202..283
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 356..422
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 355..414 e-value: 0.058 score: 15.6 coord: 274..345 e-value: 5.5E-10 score: 49.2
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 285..345 e-value: 5.1E-9 score: 36.2 coord: 363..414 e-value: 2.3E-6 score: 27.7
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 375..416 e-value: 11.0 score: 2.4 coord: 207..253 e-value: 0.0033 score: 26.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 207..254 score: 10.836942
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 77..176 e-value: 1.5E-8 score: 34.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 201..284 e-value: 1.7E-13 score: 52.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 59..193 e-value: 5.3E-7 score: 31.6
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 18..519
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 224..233
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 203..420 score: 29.798216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0025345.1	Lag0025345.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity