Lag0025331 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0025331 vs. NCBI nr
Match: XP_038894766.1 (putative E3 ubiquitin-protein ligase LIN isoform X1 [Benincasa hispida])

HSP 1 Score: 1695.2 bits (4389), Expect = 0.0e+00
Identity = 882/1011 (87.24%), Postives = 933/1011 (92.28%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RK  R KGR R APDDSVTLPIYICHDKKIIDSSKKK +
Sbjct: 1    MASLQELLTREGFEGSNFPTTRKSLRSKGRSRAAPDDSVTLPIYICHDKKIIDSSKKKAE 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K L+RNGSSVYSSKRVGSVSET LCKS     MEEPAIDEIAIRAVVSILSGYVGRYSKD
Sbjct: 61   KPLIRNGSSVYSSKRVGSVSETLLCKS-----MEEPAIDEIAIRAVVSILSGYVGRYSKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            E FRE++RKKCNPCL+RKGEMESGI SNLEMGMKSVDRLV EG GNERE RMKASRNSIG
Sbjct: 121  ESFREIMRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGQGNERELRMKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMV+TSL+SAKKS K+GA+SHLS+CAQLYLAIVYKIEKNEKVSAKHLLQVF DSPFFA
Sbjct: 181  LLNMVITSLNSAKKSTKNGAQSHLSSCAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLPELWEHFFLPHLLHLKVWYNQELE VSN E E+KDRKIKALSKVYNEHMD GTVQ
Sbjct: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALSKVYNEHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGARAPPVPVVPSPSKSIH ASR SSDSYF QSSSNKNLYHAVFGPSLDQ
Sbjct: 301  FALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFLQSSSNKNLYHAVFGPSLDQ 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWR 420
            QLAELRSGNVVAAKARSSNEKEIL+++KNYE+   V DE  NRRM SILDYR HNTDSWR
Sbjct: 361  QLAELRSGNVVAAKARSSNEKEILYSNKNYENIASVRDEHNNRRMPSILDYRGHNTDSWR 420

Query: 421  ETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILS 480
            ETVKSDYFRFFTCQN+TKEYLES+NVI KNS VRVEG++HLLSND S+AIT ICSSD+LS
Sbjct: 421  ETVKSDYFRFFTCQNITKEYLESSNVITKNSVVRVEGRNHLLSNDFSKAITAICSSDVLS 480

Query: 481  ECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAA 540
            ECEIAIRVVTKAWLDAH  + IEV LSKPPVVEGMLEVLLASDDDE+LELVISVLAELAA
Sbjct: 481  ECEIAIRVVTKAWLDAHDDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAA 540

Query: 541  KNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600
            ++EVIRQ+ILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE
Sbjct: 541  RSEVIRQIILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600

Query: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660
            FGGQLQTLFS+RCKPHEAAFYLLDQLLKGFDEDRNLENCRHLI+LGGLSLLLRRLERGEI
Sbjct: 601  FGGQLQTLFSIRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLISLGGLSLLLRRLERGEI 660

Query: 661  EERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720
            EERKNSVSII CCI+ADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS
Sbjct: 661  EERKNSVSIISCCIQADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720

Query: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFR 780
            RRTRIT LLDGLKEGWSGLGIMNILSVYLQRA PEEQPL ATILLQLDFMED LNCSIFR
Sbjct: 721  RRTRITNLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATILLQLDFMEDSLNCSIFR 780

Query: 781  EEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDS 840
            EEAIVTIITALNSR+ RE  Q++LARALLILGGRFSC+G PS ENWLL+ AGFKE+SGDS
Sbjct: 781  EEAIVTIITALNSRICREKGQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDS 840

Query: 841  FHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQSLAKASLI 900
             HS+ +YDD VQ YEEEEDVVNWQLKAATVLFN+GHKSLLSALST M SCI SLAKASLI
Sbjct: 841  SHSQPLYDDVVQLYEEEEDVVNWQLKAATVLFNHGHKSLLSALSTLMTSCILSLAKASLI 900

Query: 901  TVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKH 960
            TVSWMSRYLFV++DEKLCLMAPSILVP LIKYL++DKDVED+VLASYSLLNLSKYTECKH
Sbjct: 901  TVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDQVLASYSLLNLSKYTECKH 960

Query: 961  IFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSEQEDSHTQTSTR 1008
            IFRLFD++ LD LQNLSLVTWTAEEL  ++K  S H ++E E+SH Q STR
Sbjct: 961  IFRLFDEDTLDYLQNLSLVTWTAEELTLILKSRSMHPYTEHENSHIQRSTR 1006

BLAST of Lag0025331 vs. NCBI nr
Match: XP_038894767.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1688.3 bits (4371), Expect = 0.0e+00
Identity = 881/1011 (87.14%), Postives = 932/1011 (92.19%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RK  R KGR R APDDSVTLPIYICHDKKIIDSSKKK +
Sbjct: 1    MASLQELLTREGFEGSNFPTTRKSLRSKGRSRAAPDDSVTLPIYICHDKKIIDSSKKKAE 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K L+RNGSSVYSSKRVGSVSET LCKS     MEEPAIDEIAIRAVVSILSGYVGRYSKD
Sbjct: 61   KPLIRNGSSVYSSKRVGSVSETLLCKS-----MEEPAIDEIAIRAVVSILSGYVGRYSKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            E FRE++RKKCNPCL+RKGEMESGI SNLEMGMKSVDRLV EG GNERE RMKASRNSIG
Sbjct: 121  ESFREIMRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGQGNERELRMKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMV+TSL+SAKKS K+GA+SHLS+CAQLYLAIVYKIEKNEKVSAKHLLQVF DSPFFA
Sbjct: 181  LLNMVITSLNSAKKSTKNGAQSHLSSCAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLPELWEHFFLPHLLHLKVWYNQELE VSN E E+KDRKIKALSKVYNEHMD GTVQ
Sbjct: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALSKVYNEHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGARAPPVPVVPSPSKSIH ASR SSDSYF QSSSNKNLYHAVFGPSLDQ
Sbjct: 301  FALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFLQSSSNKNLYHAVFGPSLDQ 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWR 420
            QLAELRSGNVVAAKARSSNEKEIL+++KNYE+     DE  NRRM SILDYR HNTDSWR
Sbjct: 361  QLAELRSGNVVAAKARSSNEKEILYSNKNYENI--ASDEHNNRRMPSILDYRGHNTDSWR 420

Query: 421  ETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILS 480
            ETVKSDYFRFFTCQN+TKEYLES+NVI KNS VRVEG++HLLSND S+AIT ICSSD+LS
Sbjct: 421  ETVKSDYFRFFTCQNITKEYLESSNVITKNSVVRVEGRNHLLSNDFSKAITAICSSDVLS 480

Query: 481  ECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAA 540
            ECEIAIRVVTKAWLDAH  + IEV LSKPPVVEGMLEVLLASDDDE+LELVISVLAELAA
Sbjct: 481  ECEIAIRVVTKAWLDAHDDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAA 540

Query: 541  KNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600
            ++EVIRQ+ILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE
Sbjct: 541  RSEVIRQIILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600

Query: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660
            FGGQLQTLFS+RCKPHEAAFYLLDQLLKGFDEDRNLENCRHLI+LGGLSLLLRRLERGEI
Sbjct: 601  FGGQLQTLFSIRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLISLGGLSLLLRRLERGEI 660

Query: 661  EERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720
            EERKNSVSII CCI+ADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS
Sbjct: 661  EERKNSVSIISCCIQADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720

Query: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFR 780
            RRTRIT LLDGLKEGWSGLGIMNILSVYLQRA PEEQPL ATILLQLDFMED LNCSIFR
Sbjct: 721  RRTRITNLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATILLQLDFMEDSLNCSIFR 780

Query: 781  EEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDS 840
            EEAIVTIITALNSR+ RE  Q++LARALLILGGRFSC+G PS ENWLL+ AGFKE+SGDS
Sbjct: 781  EEAIVTIITALNSRICREKGQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDS 840

Query: 841  FHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQSLAKASLI 900
             HS+ +YDD VQ YEEEEDVVNWQLKAATVLFN+GHKSLLSALST M SCI SLAKASLI
Sbjct: 841  SHSQPLYDDVVQLYEEEEDVVNWQLKAATVLFNHGHKSLLSALSTLMTSCILSLAKASLI 900

Query: 901  TVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKH 960
            TVSWMSRYLFV++DEKLCLMAPSILVP LIKYL++DKDVED+VLASYSLLNLSKYTECKH
Sbjct: 901  TVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDQVLASYSLLNLSKYTECKH 960

Query: 961  IFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSEQEDSHTQTSTR 1008
            IFRLFD++ LD LQNLSLVTWTAEEL  ++K  S H ++E E+SH Q STR
Sbjct: 961  IFRLFDEDTLDYLQNLSLVTWTAEELTLILKSRSMHPYTEHENSHIQRSTR 1004

BLAST of Lag0025331 vs. NCBI nr
Match: XP_004145484.2 (putative E3 ubiquitin-protein ligase LIN-2 isoform X2 [Cucumis sativus])

HSP 1 Score: 1667.5 bits (4317), Expect = 0.0e+00
Identity = 871/1012 (86.07%), Postives = 932/1012 (92.09%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RK SRPKGR RTAPDDSVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K LVRNGSSVYSSKRVGSVSET  CKS     MEEPAIDEIAIRAVVSILSGYVGRYSKD
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKS-----MEEPAIDEIAIRAVVSILSGYVGRYSKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            E FRE+VRKKCNPCL+RKGEMESGI SNLEMGMKSVDRLV EGHGNERE R+KASRNSIG
Sbjct: 121  ENFREIVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMV+TSL SAK S K+GA SHLSACAQLYLAIV KIEKNE+VSAKHLLQVF DSP+FA
Sbjct: 181  LLNMVITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLP+LWEHFFLPHLLHLKVWYNQELE VSN E E+KDRKIKAL+KVYN+HMD GTVQ
Sbjct: 241  RTHLLPDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGARAPPVPVVPSPSKSIH ASR SSDSYFSQ SSNKNLYHAVFGPSLDQ
Sbjct: 301  FALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQ 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWR 420
            QLAELR GN+VAA ARSS+EKEILF+DK+YE+S  V DE  NRRMSS+LDYRSHNT+SWR
Sbjct: 361  QLAELRRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWR 420

Query: 421  ETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILS 480
            ETVKSDYFRFFTCQN+TKEYLES+NVI KNS V+VEG++HLLSND S+AIT ICSSDILS
Sbjct: 421  ETVKSDYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILS 480

Query: 481  ECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAA 540
            ECEIA+RVVTKAWLDAHG S IEV LS+PPVVEGMLEVLLASDDDE+LELVISVLAELAA
Sbjct: 481  ECEIAVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAA 540

Query: 541  KNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600
            ++E+I+QMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE
Sbjct: 541  RSEIIQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600

Query: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660
            FGGQLQTLFSV+CKPHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLRRLERGEI
Sbjct: 601  FGGQLQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEI 660

Query: 661  EERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720
            EERKNSVSII CCI+ADGSCRNYLAENLNKASLLEL+VHESNKNSDR GLALLVDLLCLS
Sbjct: 661  EERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLS 720

Query: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFR 780
            RRTRITKLLDGLKEGWSGLGIMNILSVYLQRA PEEQPL AT LLQLDFMED LNCSIFR
Sbjct: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFR 780

Query: 781  EEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDS 840
            EEAIVTIITALN+R+  E AQ++LARALLILGGRFSC+G PS ENWLL+ AGFKE+SGDS
Sbjct: 781  EEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDS 840

Query: 841  FHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQ-SLAKASL 900
             HSKH+YDD VQ YEEEE+V+NWQLKAA+VLFN+GHKSLLS+LSTSM SCI+ SLAKA L
Sbjct: 841  SHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACL 900

Query: 901  ITVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECK 960
            IT+SWMSRYLFV+R+EKLCLMAPSILVP LIKYL++DK VED+VLASYSLLNL KYTECK
Sbjct: 901  ITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECK 960

Query: 961  HIFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSEQEDSHTQTSTR 1008
            HIFRLFDDEALD L+NLSLVTWTAEELI +I  GS   ++E+E+S  Q STR
Sbjct: 961  HIFRLFDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTR 1007

BLAST of Lag0025331 vs. NCBI nr
Match: XP_022142564.1 (putative E3 ubiquitin-protein ligase LIN-1 [Momordica charantia])

HSP 1 Score: 1659.4 bits (4296), Expect = 0.0e+00
Identity = 866/1012 (85.57%), Postives = 929/1012 (91.80%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RKPSRP+GRRRT  D SVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGTNYPKTRKPSRPRGRRRTPADGSVTLPIYICHDKKFIDSSKKKAD 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K  VRNGSSVYSSKRVGSVSE+SLCKS     MEEPAID+IAIRAVVSILSGYVG Y+KD
Sbjct: 61   KAFVRNGSSVYSSKRVGSVSESSLCKS-----MEEPAIDDIAIRAVVSILSGYVGSYAKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            EIFRE VRKKCN CLVR GEM  GI SNLEMGMKSVDRLV EGHGNE+E R+KASRNSIG
Sbjct: 121  EIFRETVRKKCNLCLVRSGEMGVGICSNLEMGMKSVDRLVEEGHGNEKELRVKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMVV SL+S  +SM++G+ S LSACA+LYLAIVYKIEKNEK+SAKHLLQVF DSPF A
Sbjct: 181  LLNMVVASLNSV-RSMENGS-SDLSACAKLYLAIVYKIEKNEKISAKHLLQVFCDSPFLA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLPELWEHFFLPHLLHLKVWY+QELE VSN+E E+KDR+IKALSKVYNEHMD GTVQ
Sbjct: 241  RTHLLPELWEHFFLPHLLHLKVWYSQELEFVSNVECEHKDRRIKALSKVYNEHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGA+APPVPVVPSPSKSIHGASR SSDSYFSQSSSNKNLYHAVFGPSL++
Sbjct: 301  FALYYIQWLKDGAKAPPVPVVPSPSKSIHGASRRSSDSYFSQSSSNKNLYHAVFGPSLEE 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWR 420
            Q+ ELRSGN+ AAK RSSNEKEILFA+KNYESS  VH+EL N RMSS+LDYR  N DSWR
Sbjct: 361  QVIELRSGNMAAAKTRSSNEKEILFAEKNYESSASVHEELSNHRMSSVLDYRHQNADSWR 420

Query: 421  ETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILS 480
            ETVKSDY RFFTCQNV KEYLE++NVI +NS+VRV+GKSHLLSNDLSRAI+TICSSDILS
Sbjct: 421  ETVKSDYLRFFTCQNVAKEYLENSNVITRNSAVRVDGKSHLLSNDLSRAISTICSSDILS 480

Query: 481  ECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAA 540
            ECEIAIRVVTKAWLDAHG +AIEV LSKPPVVEGMLEVLLASDDDE+LELVISVLAELAA
Sbjct: 481  ECEIAIRVVTKAWLDAHGDTAIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAA 540

Query: 541  KNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600
            ++EVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE
Sbjct: 541  RDEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600

Query: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660
            FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI
Sbjct: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660

Query: 661  EERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720
            EERKN+VSII CCI+ADGSCRNYL +NLNK SLLELIVHESNK+SDRCGLALLVDLLCLS
Sbjct: 661  EERKNAVSIISCCIQADGSCRNYLVDNLNKTSLLELIVHESNKSSDRCGLALLVDLLCLS 720

Query: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFR 780
            RRTRIT+LLDGLKEGWSGLGIMNILSVYLQRA PEEQPL A ILLQLDFMED  NCSI+R
Sbjct: 721  RRTRITRLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAAILLQLDFMEDTQNCSIYR 780

Query: 781  EEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDS 840
            EEAIVTIITAL SR+SRE  QE+LARALLILGGRF  +G PS ENWLL +AGFKESS DS
Sbjct: 781  EEAIVTIITALKSRISREEVQENLARALLILGGRFLYTGEPSAENWLLLRAGFKESSEDS 840

Query: 841  FHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQSLAKASLI 900
            FHSKHVYDDFVQSYEEEEDV NWQLKAATVLFN+GHKSLLSALS S++S I SLAKASL+
Sbjct: 841  FHSKHVYDDFVQSYEEEEDVENWQLKAATVLFNHGHKSLLSALSISLSSSIPSLAKASLV 900

Query: 901  TVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKH 960
            TVSWMS YLFVVRDEKL L+ PSILVP LIKYL+YDKDVEDRVLASYSLLNLSKYTECKH
Sbjct: 901  TVSWMSTYLFVVRDEKLQLITPSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKH 960

Query: 961  IFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSEQEDSHTQTSTRI 1009
            IFRLFD+E LDRLQNLSLVTWTAEEL+ +++ GS HQ++++E+S+TQ S RI
Sbjct: 961  IFRLFDEEVLDRLQNLSLVTWTAEELVLIVRSGSAHQYTKRENSYTQRSRRI 1005

BLAST of Lag0025331 vs. NCBI nr
Match: XP_031744408.1 (putative E3 ubiquitin-protein ligase LIN-2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1649.8 bits (4271), Expect = 0.0e+00
Identity = 871/1047 (83.19%), Postives = 932/1047 (89.02%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RK SRPKGR RTAPDDSVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K LVRNGSSVYSSKRVGSVSET  CKS     MEEPAIDEIAIRAVVSILSGYVGRYSKD
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKS-----MEEPAIDEIAIRAVVSILSGYVGRYSKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            E FRE+VRKKCNPCL+RKGEMESGI SNLEMGMKSVDRLV EGHGNERE R+KASRNSIG
Sbjct: 121  ENFREIVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMV+TSL SAK S K+GA SHLSACAQLYLAIV KIEKNE+VSAKHLLQVF DSP+FA
Sbjct: 181  LLNMVITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLP+LWEHFFLPHLLHLKVWYNQELE VSN E E+KDRKIKAL+KVYN+HMD GTVQ
Sbjct: 241  RTHLLPDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGARAPPVPVVPSPSKSIH ASR SSDSYFSQ SSNKNLYHAVFGPSLDQ
Sbjct: 301  FALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQ 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPV------------------------ 420
            QLAELR GN+VAA ARSS+EKEILF+DK+YE+S  V                        
Sbjct: 361  QLAELRRGNLVAAMARSSHEKEILFSDKHYENSASVQVCNSISIFNIESFGSWLEKSRCT 420

Query: 421  -----------HDELGNRRMSSILDYRSHNTDSWRETVKSDYFRFFTCQNVTKEYLESTN 480
                        DE  NRRMSS+LDYRSHNT+SWRETVKSDYFRFFTCQN+TKEYLES+N
Sbjct: 421  VFIFLRSLLFLQDEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQNITKEYLESSN 480

Query: 481  VIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILSECEIAIRVVTKAWLDAHGHSAIEVT 540
            VI KNS V+VEG++HLLSND S+AIT ICSSDILSECEIA+RVVTKAWLDAHG S IEV 
Sbjct: 481  VITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVA 540

Query: 541  LSKPPVVEGMLEVLLASDDDEVLELVISVLAELAAKNEVIRQMILNSDPQLQVFLKLLKS 600
            LS+PPVVEGMLEVLLASDDDE+LELVISVLAELAA++E+I+QMILNSDPQLQVFLKLLKS
Sbjct: 541  LSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKS 600

Query: 601  SSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQ 660
            SSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSV+CKPHEAAFYLLDQ
Sbjct: 601  SSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQ 660

Query: 661  LLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEIEERKNSVSIILCCIRADGSCRNYLA 720
            LLKGFDEDRNLEN RHLIALGGLSLLLRRLERGEIEERKNSVSII CCI+ADGSCRNYLA
Sbjct: 661  LLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLA 720

Query: 721  ENLNKASLLELIVHESNKNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNIL 780
            ENLNKASLLEL+VHESNKNSDR GLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNIL
Sbjct: 721  ENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNIL 780

Query: 781  SVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFREEAIVTIITALNSRLSRENAQESLA 840
            SVYLQRA PEEQPL AT LLQLDFMED LNCSIFREEAIVTIITALN+R+  E AQ++LA
Sbjct: 781  SVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLA 840

Query: 841  RALLILGGRFSCSGVPSVENWLLQQAGFKESSGDSFHSKHVYDDFVQSYEEEEDVVNWQL 900
            RALLILGGRFSC+G PS ENWLL+ AGFKE+SGDS HSKH+YDD VQ YEEEE+V+NWQL
Sbjct: 841  RALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHLYDDVVQLYEEEEEVMNWQL 900

Query: 901  KAATVLFNYGHKSLLSALSTSMASCIQ-SLAKASLITVSWMSRYLFVVRDEKLCLMAPSI 960
            KAA+VLFN+GHKSLLS+LSTSM SCI+ SLAKA LIT+SWMSRYLFV+R+EKLCLMAPSI
Sbjct: 901  KAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLMAPSI 960

Query: 961  LVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDDEALDRLQNLSLVTWTAE 1008
            LVP LIKYL++DK VED+VLASYSLLNL KYTECKHIFRLFDDEALD L+NLSLVTWTAE
Sbjct: 961  LVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVTWTAE 1020

BLAST of Lag0025331 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 3.6e-58
Identity = 163/544 (29.96%), Postives = 285/544 (52.39%), Query Frame = 0

Query: 447  SSVRVEGKSHLLSNDLSRAITTICSSDILSECEIAIRVVTKAWLDAHGHSAIEVTLSKPP 506
            +SV  +     + N L   I+++C+S+ L ECE A+  + +   D+  +  I   LSKP 
Sbjct: 645  TSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPT 704

Query: 507  VVEGMLEVLLASDDDEVLELVISVLAELAAKNEVIRQMILNSDPQLQVFLKLLKSSSLFL 566
            ++ G++E+L AS + EVL   I +L+EL   ++ + + + + D        LLK+     
Sbjct: 705  IINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LA 764

Query: 567  KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGF 626
            +A++L+Y  +P   Q+ + E +P ++ V++   +    F +   P +AA  +L+Q L G 
Sbjct: 765  EAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGG 824

Query: 627  DEDRNLENCRHLIALGGLSLLLRRLERGEIEERKNSVSIILCCIRADGSCRNYLAENLNK 686
            DE     N   +I+  G+  L++ LER  +E R++ VS++LCC++A+ SC+N +A  +  
Sbjct: 825  DEYSRSLNASSVISANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIANRIEL 884

Query: 687  ASLLELIVHESNKNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQ 746
            + +LEL  H  N +     +  L +L+ L+RRT   +LL  +K+       M+   VYLQ
Sbjct: 885  SPVLELF-HSGNDSVRGTCVEFLSELVQLNRRTSCNQLLHTIKDE-GAFSTMHTFLVYLQ 944

Query: 747  RAHPEEQPLAATILLQLDFMEDPLNCSIFREEAIVTIITALNSRLSRENAQESLARALLI 806
             A  E Q   A++LLQLD + +P   SI+REEA+ T+I AL  +    N Q     ALL 
Sbjct: 945  MAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQK-DFSNTQMKALDALLF 1004

Query: 807  LGGRFSCSGVPSVENWLLQQAGFKESSGDSFHSKHV---YDDFVQSYEEEEDVVN-WQLK 866
            L G  S SG    E WLL+ AGF +        + +    +D +++ E+E++ +N WQ +
Sbjct: 1005 LIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKR 1064

Query: 867  AATVLFNYGHKSLLSALSTSMASCIQSLAKASLITVSWMSRYLFVVRDEKLCLMAPSILV 926
             A+VL N+ + S+  AL   + S    +AK+ L+  +W++R L+ + D  +  +A   L+
Sbjct: 1065 IASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLL 1124

Query: 927  PTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDDEALDRLQNLSLVTWTAEEL 986
              +IK L   K +ED +L + SL           + R++       L+ L   +  A ++
Sbjct: 1125 EEVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADI 1181

BLAST of Lag0025331 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 225.7 bits (574), Expect = 2.3e-57
Identity = 161/544 (29.60%), Postives = 286/544 (52.57%), Query Frame = 0

Query: 447  SSVRVEGKSHLLSNDLSRAITTICSSDILSECEIAIRVVTKAWLDAHGHSAIEVTLSKPP 506
            +SV  +     + N L   I+++C+S+ L ECE A+  + +   D+  +  I   LSKP 
Sbjct: 645  TSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPT 704

Query: 507  VVEGMLEVLLASDDDEVLELVISVLAELAAKNEVIRQMILNSDPQLQVFLKLLKSSSLFL 566
            ++ G++E+L AS + EVL   I +L+EL   ++ + + + + D        LLK+     
Sbjct: 705  IINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LA 764

Query: 567  KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGF 626
            +A++L+Y  +P   Q+ + E +P ++ V++   +    F +   P +AA  +L+Q L G 
Sbjct: 765  EAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGG 824

Query: 627  DEDRNLENCRHLIALGGLSLLLRRLERGEIEERKNSVSIILCCIRADGSCRNYLAENLNK 686
            DE     N   +I+  G+  L++ LER  +E R++ VS++LCC++A+ SC+N +A  +  
Sbjct: 825  DEYSRSLNASSVISANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIANRIEL 884

Query: 687  ASLLELIVHESNKNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQ 746
            + +LEL  H  N +     +  L +L+ L+RRT   ++L  +K+       M+   VYLQ
Sbjct: 885  SPVLELF-HSGNDSVRGTCVEFLSELVQLNRRTSCNQILHTIKDE-GAFSTMHTFLVYLQ 944

Query: 747  RAHPEEQPLAATILLQLDFMEDPLNCSIFREEAIVTIITALNSRLSRENAQESLARALLI 806
             A  E Q   A++LLQLD + +P   SI+REEA+ T+I AL  +    N Q     ALL 
Sbjct: 945  MAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQK-DFSNTQMKALDALLF 1004

Query: 807  LGGRFSCSGVPSVENWLLQQAGFKESSGDSFHSKHV---YDDFVQSYEEEEDVVN-WQLK 866
            L G  S SG    E WLL+ AGF +        + +    +D +++ E+E++ +N WQ +
Sbjct: 1005 LIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKR 1064

Query: 867  AATVLFNYGHKSLLSALSTSMASCIQSLAKASLITVSWMSRYLFVVRDEKLCLMAPSILV 926
             A+VL N+ + S+  AL   + S    +AK+ L+  +W++  L+ + D  +  +A   L+
Sbjct: 1065 IASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLL 1124

Query: 927  PTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDDEALDRLQNLSLVTWTAEEL 986
              +I  L   K++E+++LA+ +L             R++       L+ L   +  A ++
Sbjct: 1125 EEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDI 1181

BLAST of Lag0025331 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 4.0e-57
Identity = 169/579 (29.19%), Postives = 299/579 (51.64%), Query Frame = 0

Query: 412  TDSWRETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICS 471
            TDS  +  K DY R               +V A  +SV  +     + N L+  IT++C+
Sbjct: 619  TDSPSQKYKDDYIR------QRNNRFTRVSVGASPTSVLSQAAVETIINSLTPYITSLCT 678

Query: 472  SDILSECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVL 531
            S+ L +CE A+  + + W D+     I   LSKP VV G++E+L AS + EVL   I +L
Sbjct: 679  SENLQDCEQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASLNREVLRRSIYIL 738

Query: 532  AELAAKNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLV 591
            +EL   +E + + + + D        LLK+     +A++L+Y  +P   Q+   E +P +
Sbjct: 739  SELIFSDERVGETLNSVDSDFDCLAMLLKNG--LAEAALLIYQLRPVFAQLSEHELIPSL 798

Query: 592  LRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRL 651
            ++V++   +    F +   P  AA  +L+Q+L G DE     N   +I+  G+  +++ L
Sbjct: 799  IQVIQNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVISANGIPAIVKYL 858

Query: 652  ERGEIEERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVD 711
            ++   E R+  +SI+LCC++A+ SC++ +A  +  + +LEL  H  N +     +  L +
Sbjct: 859  DK--TEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLELF-HAGNDSVRGICVEFLSE 918

Query: 712  LLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLN 771
            L+ L+RRT   + L  +K+       M+   VYLQ A  E Q   A++LLQLD + +P  
Sbjct: 919  LVRLNRRTSSNQTLQIIKDE-GAFSTMHTFLVYLQMAPMEHQIAVASLLLQLDLLAEPRK 978

Query: 772  CSIFREEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKE 831
             SI+REEA+ T+I AL  +    N  ++L  ALL L G  + SG    E  LL+ AGF +
Sbjct: 979  MSIYREEAVETLIEALWQKDFSNNQMKAL-DALLFLIGHVTSSGKSYTEAGLLKIAGFDQ 1038

Query: 832  SSGDSFHSK---HVYDDFVQSYEEEEDVV-NWQLKAATVLFNYGHKSLLSALSTSMASCI 891
                   ++   H  +DF+++ E+E++ + +WQ + A+VL N+ + S+  AL   + S  
Sbjct: 1039 PYNVLMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVASVLCNHENGSIFQALEECLKSNS 1098

Query: 892  QSLAKASLITVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLN 951
              +AK+ L+  +W++  LF + D  +  +A   L+  L+  L   K++E+++LAS +L +
Sbjct: 1099 LKMAKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKNLEEKILASLALKS 1158

Query: 952  LSKYTECKHIFRLFDDEALDRLQNLSLVTWTAEELISVI 987
                     + R++       L+ L   +  A +++  +
Sbjct: 1159 FISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADILKAL 1184

BLAST of Lag0025331 vs. ExPASy TrEMBL
Match: A0A0A0LYR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G713660 PE=4 SV=1)

HSP 1 Score: 1667.5 bits (4317), Expect = 0.0e+00
Identity = 871/1012 (86.07%), Postives = 932/1012 (92.09%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RK SRPKGR RTAPDDSVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K LVRNGSSVYSSKRVGSVSET  CKS     MEEPAIDEIAIRAVVSILSGYVGRYSKD
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKS-----MEEPAIDEIAIRAVVSILSGYVGRYSKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            E FRE+VRKKCNPCL+RKGEMESGI SNLEMGMKSVDRLV EGHGNERE R+KASRNSIG
Sbjct: 121  ENFREIVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMV+TSL SAK S K+GA SHLSACAQLYLAIV KIEKNE+VSAKHLLQVF DSP+FA
Sbjct: 181  LLNMVITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLP+LWEHFFLPHLLHLKVWYNQELE VSN E E+KDRKIKAL+KVYN+HMD GTVQ
Sbjct: 241  RTHLLPDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGARAPPVPVVPSPSKSIH ASR SSDSYFSQ SSNKNLYHAVFGPSLDQ
Sbjct: 301  FALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQ 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWR 420
            QLAELR GN+VAA ARSS+EKEILF+DK+YE+S  V DE  NRRMSS+LDYRSHNT+SWR
Sbjct: 361  QLAELRRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWR 420

Query: 421  ETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILS 480
            ETVKSDYFRFFTCQN+TKEYLES+NVI KNS V+VEG++HLLSND S+AIT ICSSDILS
Sbjct: 421  ETVKSDYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILS 480

Query: 481  ECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAA 540
            ECEIA+RVVTKAWLDAHG S IEV LS+PPVVEGMLEVLLASDDDE+LELVISVLAELAA
Sbjct: 481  ECEIAVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAA 540

Query: 541  KNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600
            ++E+I+QMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE
Sbjct: 541  RSEIIQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600

Query: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660
            FGGQLQTLFSV+CKPHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLRRLERGEI
Sbjct: 601  FGGQLQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEI 660

Query: 661  EERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720
            EERKNSVSII CCI+ADGSCRNYLAENLNKASLLEL+VHESNKNSDR GLALLVDLLCLS
Sbjct: 661  EERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLS 720

Query: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFR 780
            RRTRITKLLDGLKEGWSGLGIMNILSVYLQRA PEEQPL AT LLQLDFMED LNCSIFR
Sbjct: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFR 780

Query: 781  EEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDS 840
            EEAIVTIITALN+R+  E AQ++LARALLILGGRFSC+G PS ENWLL+ AGFKE+SGDS
Sbjct: 781  EEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDS 840

Query: 841  FHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQ-SLAKASL 900
             HSKH+YDD VQ YEEEE+V+NWQLKAA+VLFN+GHKSLLS+LSTSM SCI+ SLAKA L
Sbjct: 841  SHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACL 900

Query: 901  ITVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECK 960
            IT+SWMSRYLFV+R+EKLCLMAPSILVP LIKYL++DK VED+VLASYSLLNL KYTECK
Sbjct: 901  ITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECK 960

Query: 961  HIFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSEQEDSHTQTSTR 1008
            HIFRLFDDEALD L+NLSLVTWTAEELI +I  GS   ++E+E+S  Q STR
Sbjct: 961  HIFRLFDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTR 1007

BLAST of Lag0025331 vs. ExPASy TrEMBL
Match: A0A6J1CLV0 (putative E3 ubiquitin-protein ligase LIN-1 OS=Momordica charantia OX=3673 GN=LOC111012647 PE=4 SV=1)

HSP 1 Score: 1659.4 bits (4296), Expect = 0.0e+00
Identity = 866/1012 (85.57%), Postives = 929/1012 (91.80%), Query Frame = 0

Query: 1    MASLQELLTREGFEGRN----RKPSRPKGRRRTAPDDSVTLPIYICHDKKIIDSSKKKVD 60
            MASLQELLTREGFEG N    RKPSRP+GRRRT  D SVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGTNYPKTRKPSRPRGRRRTPADGSVTLPIYICHDKKFIDSSKKKAD 60

Query: 61   KTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYVGRYSKD 120
            K  VRNGSSVYSSKRVGSVSE+SLCKS     MEEPAID+IAIRAVVSILSGYVG Y+KD
Sbjct: 61   KAFVRNGSSVYSSKRVGSVSESSLCKS-----MEEPAIDDIAIRAVVSILSGYVGSYAKD 120

Query: 121  EIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIG 180
            EIFRE VRKKCN CLVR GEM  GI SNLEMGMKSVDRLV EGHGNE+E R+KASRNSIG
Sbjct: 121  EIFRETVRKKCNLCLVRSGEMGVGICSNLEMGMKSVDRLVEEGHGNEKELRVKASRNSIG 180

Query: 181  LLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFA 240
            LLNMVV SL+S  +SM++G+ S LSACA+LYLAIVYKIEKNEK+SAKHLLQVF DSPF A
Sbjct: 181  LLNMVVASLNSV-RSMENGS-SDLSACAKLYLAIVYKIEKNEKISAKHLLQVFCDSPFLA 240

Query: 241  RTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQ 300
            RTHLLPELWEHFFLPHLLHLKVWY+QELE VSN+E E+KDR+IKALSKVYNEHMD GTVQ
Sbjct: 241  RTHLLPELWEHFFLPHLLHLKVWYSQELEFVSNVECEHKDRRIKALSKVYNEHMDRGTVQ 300

Query: 301  FAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQ 360
            FA+YYIQWLKDGA+APPVPVVPSPSKSIHGASR SSDSYFSQSSSNKNLYHAVFGPSL++
Sbjct: 301  FALYYIQWLKDGAKAPPVPVVPSPSKSIHGASRRSSDSYFSQSSSNKNLYHAVFGPSLEE 360

Query: 361  QLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWR 420
            Q+ ELRSGN+ AAK RSSNEKEILFA+KNYESS  VH+EL N RMSS+LDYR  N DSWR
Sbjct: 361  QVIELRSGNMAAAKTRSSNEKEILFAEKNYESSASVHEELSNHRMSSVLDYRHQNADSWR 420

Query: 421  ETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILS 480
            ETVKSDY RFFTCQNV KEYLE++NVI +NS+VRV+GKSHLLSNDLSRAI+TICSSDILS
Sbjct: 421  ETVKSDYLRFFTCQNVAKEYLENSNVITRNSAVRVDGKSHLLSNDLSRAISTICSSDILS 480

Query: 481  ECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAA 540
            ECEIAIRVVTKAWLDAHG +AIEV LSKPPVVEGMLEVLLASDDDE+LELVISVLAELAA
Sbjct: 481  ECEIAIRVVTKAWLDAHGDTAIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAA 540

Query: 541  KNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600
            ++EVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE
Sbjct: 541  RDEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLE 600

Query: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660
            FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI
Sbjct: 601  FGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEI 660

Query: 661  EERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLS 720
            EERKN+VSII CCI+ADGSCRNYL +NLNK SLLELIVHESNK+SDRCGLALLVDLLCLS
Sbjct: 661  EERKNAVSIISCCIQADGSCRNYLVDNLNKTSLLELIVHESNKSSDRCGLALLVDLLCLS 720

Query: 721  RRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFR 780
            RRTRIT+LLDGLKEGWSGLGIMNILSVYLQRA PEEQPL A ILLQLDFMED  NCSI+R
Sbjct: 721  RRTRITRLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAAILLQLDFMEDTQNCSIYR 780

Query: 781  EEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDS 840
            EEAIVTIITAL SR+SRE  QE+LARALLILGGRF  +G PS ENWLL +AGFKESS DS
Sbjct: 781  EEAIVTIITALKSRISREEVQENLARALLILGGRFLYTGEPSAENWLLLRAGFKESSEDS 840

Query: 841  FHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQSLAKASLI 900
            FHSKHVYDDFVQSYEEEEDV NWQLKAATVLFN+GHKSLLSALS S++S I SLAKASL+
Sbjct: 841  FHSKHVYDDFVQSYEEEEDVENWQLKAATVLFNHGHKSLLSALSISLSSSIPSLAKASLV 900

Query: 901  TVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKH 960
            TVSWMS YLFVVRDEKL L+ PSILVP LIKYL+YDKDVEDRVLASYSLLNLSKYTECKH
Sbjct: 901  TVSWMSTYLFVVRDEKLQLITPSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKH 960

Query: 961  IFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSEQEDSHTQTSTRI 1009
            IFRLFD+E LDRLQNLSLVTWTAEEL+ +++ GS HQ++++E+S+TQ S RI
Sbjct: 961  IFRLFDEEVLDRLQNLSLVTWTAEELVLIVRSGSAHQYTKRENSYTQRSRRI 1005

BLAST of Lag0025331 vs. ExPASy TrEMBL
Match: A0A6J1JWN9 (putative E3 ubiquitin-protein ligase LIN isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490369 PE=4 SV=1)

HSP 1 Score: 1582.8 bits (4097), Expect = 0.0e+00
Identity = 830/1007 (82.42%), Postives = 903/1007 (89.67%), Query Frame = 0

Query: 1   MASLQELLTREGFEG----RNRKPSRPKG------RRRTAPDDSVTLPIYICHDKKIIDS 60
           MASLQELLTREGFEG     NRKPSRP G      RRRT  D SVTLPIYICHDKKIIDS
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGRRRRRRTVSDGSVTLPIYICHDKKIIDS 60

Query: 61  SKKKVDKTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGYV 120
           SKKK+DK LVRNGSSVYSSKRVGSVSETSLCKSMEG K+EEPAID IAIRAVVSILSGYV
Sbjct: 61  SKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSILSGYV 120

Query: 121 GRYSKDEIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKA 180
           GRY KDE FREMVRKKC  C         GI  NLEMGMKSVDRLV E +GNE+E R+KA
Sbjct: 121 GRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEKELRVKA 180

Query: 181 SRNSIGLLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFS 240
           SRNSIGLLNMV+    SA K+MK+G RSHLSACA LYLAIVYKIEKNEKVSAKH+LQ+F 
Sbjct: 181 SRNSIGLLNMVI----SATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFC 240

Query: 241 DSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEHM 300
           D PFFARTHLLPELWEHFFLPHLLHLKVWYNQELE VSNL+ E+K+R IKALSKVYNE+M
Sbjct: 241 DYPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYM 300

Query: 301 DSGTVQFAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQ-SSSNKNLYHAV 360
           D GT QFA+YYIQWLKDGA+APPVPVV  PSKSIHG SR SSDSYFSQ SSSNKNLYHAV
Sbjct: 301 DMGTAQFAVYYIQWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSNKNLYHAV 360

Query: 361 FGPSLDQQLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYRS 420
           FGPSLDQQLAELRS    AAKARSSN           ESSVPVHDEL N   SSIL Y++
Sbjct: 361 FGPSLDQQLAELRS----AAKARSSN-----------ESSVPVHDELSNGITSSILRYKT 420

Query: 421 HNTDSWRETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITTI 480
           HNTDSWRETVKSDYF FFTCQN+TKE+ ES+N++AKN ++RVEG++H LS+ +SRAI TI
Sbjct: 421 HNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKN-AIRVEGRNHNLSSVVSRAIATI 480

Query: 481 CSSDILSECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVIS 540
           CSSDIL++CEIA+RVVTKAWLDAHG +AIEV LSKPPV+EGMLEVLLAS+DDE+LELVIS
Sbjct: 481 CSSDILNDCEIAVRVVTKAWLDAHGDAAIEVALSKPPVIEGMLEVLLASNDDEILELVIS 540

Query: 541 VLAELAAKNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLP 600
           VLAELAAKNE+IRQ+IL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEWLP
Sbjct: 541 VLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLP 600

Query: 601 LVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLR 660
           LVLRVLEFGGQLQTLFSVRC PHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLR
Sbjct: 601 LVLRVLEFGGQLQTLFSVRCMPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLR 660

Query: 661 RLERGEIEERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALL 720
           RLE GEIEERK SVSII+CCIRADGSCRNYLAEN+NKASLLELIVHESN NSDRCGLALL
Sbjct: 661 RLEEGEIEERKKSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLALL 720

Query: 721 VDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMEDP 780
           VDLLCLSRRTRI++LLDGLK+GW+GL IMN+LSVYLQRA PEEQPL ATILLQLDFMEDP
Sbjct: 721 VDLLCLSRRTRISRLLDGLKDGWNGLSIMNVLSVYLQRARPEEQPLVATILLQLDFMEDP 780

Query: 781 LNCSIFREEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAGF 840
           LNC+IFREEAIVTIIT+LN+R+S E  QE+LARALLILGGRFSC+G P++ENWLLQQAGF
Sbjct: 781 LNCTIFREEAIVTIITSLNARISHEKTQENLARALLILGGRFSCTGEPTIENWLLQQAGF 840

Query: 841 KESSGDSFHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQS 900
           KE+SG SFHSKH+YDDFVQSYEEEE+VVNWQLKAATV FN+GHKSLLSALSTSM SCI S
Sbjct: 841 KENSGGSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCIPS 900

Query: 901 LAKASLITVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLS 960
           LAKA L+ VSWMS+YLFVV D+KLCLMAPSILVP LIKYL+YDK+VED+VLASYSLLNLS
Sbjct: 901 LAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKEVEDQVLASYSLLNLS 960

Query: 961 KYTECKHIFRLFDDEALDRLQNLSLVTWTAEELISVIKRGSTHQHSE 997
           KYTECKHIFRLFD+EALD L+NLSLVTWTAEELIS+++ GSTHQ++E
Sbjct: 961 KYTECKHIFRLFDEEALDHLRNLSLVTWTAEELISIVESGSTHQYTE 978

BLAST of Lag0025331 vs. ExPASy TrEMBL
Match: A0A6J1GRP8 (putative E3 ubiquitin-protein ligase LIN OS=Cucurbita moschata OX=3662 GN=LOC111456892 PE=4 SV=1)

HSP 1 Score: 1564.3 bits (4049), Expect = 0.0e+00
Identity = 823/1009 (81.57%), Postives = 900/1009 (89.20%), Query Frame = 0

Query: 1   MASLQELLTREGFEG----RNRKPSRPKG-------RRRTAPDDSVTLPIYICHDKKIID 60
           MASLQELLTREGFEG     NRKP RP G       RRRT  D SVTLPIYICHDKKIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPLRPNGGGGGRRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKVDKTLVRNGSSVYSSKRVGSVSETSLCKSMEGPKMEEPAIDEIAIRAVVSILSGY 120
           SSKKK+DK LVRNGSSVYSSKRVGSVSETSLCKSMEG K+EEPAID IAIRAVVS+LSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120

Query: 121 VGRYSKDEIFREMVRKKCNPCLVRKGEMESGIFSNLEMGMKSVDRLVVEGHGNEREFRMK 180
           VGRY KDE FREMVRKKC  C         GI  NLEMGMKSVDRLV E +GNE+E R+K
Sbjct: 121 VGRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEQELRVK 180

Query: 181 ASRNSIGLLNMVVTSLSSAKKSMKHGARSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVF 240
           ASRNSIGLLNMV+    SA K+MK+G RSHLSACA LYLAIVYKIEKNEKVSAKH+LQ+F
Sbjct: 181 ASRNSIGLLNMVI----SATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLF 240

Query: 241 SDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEIVSNLESEYKDRKIKALSKVYNEH 300
            D PFFART+LLPELWEHFFLPHLLHLKVWYNQELE VSNL+ E+K+R IKALSKVYNE+
Sbjct: 241 CDYPFFARTYLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEY 300

Query: 301 MDSGTVQFAMYYIQWLKDGARAPPVPVVPSPSKSIHGASRSSSDSYFSQ-SSSNKNLYHA 360
           MD GT QFA+YY+QWLKDGA+APPVPVV  PSKSI G SR SSDSYFSQ SSSNKNLYHA
Sbjct: 301 MDMGTAQFAVYYVQWLKDGAKAPPVPVVSLPSKSISGMSRRSSDSYFSQSSSSNKNLYHA 360

Query: 361 VFGPSLDQQLAELRSGNVVAAKARSSNEKEILFADKNYESSVPVHDELGNRRMSSILDYR 420
           VFGPSLDQQLAELR+    AAKARSSNEK           SVPVHDE  N   SSIL Y+
Sbjct: 361 VFGPSLDQQLAELRT----AAKARSSNEK-----------SVPVHDEPSNGISSSILCYK 420

Query: 421 SHNTDSWRETVKSDYFRFFTCQNVTKEYLESTNVIAKNSSVRVEGKSHLLSNDLSRAITT 480
           +HNTDSWRETVKSDYF FFTCQN+TKE+ ES+N++AKN ++RVEG++H LS+ ++RAI T
Sbjct: 421 THNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKN-AIRVEGRNHNLSSVVNRAIAT 480

Query: 481 ICSSDILSECEIAIRVVTKAWLDAHGHSAIEVTLSKPPVVEGMLEVLLASDDDEVLELVI 540
           ICSSDIL++CEIA+RVVTKAWLDAHG +AIE  LSKP VVEGMLEVLLAS+DDE+LELVI
Sbjct: 481 ICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAVLSKPLVVEGMLEVLLASNDDEILELVI 540

Query: 541 SVLAELAAKNEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWL 600
           SVLAELAAKNE+IRQ+IL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEWL
Sbjct: 541 SVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEWL 600

Query: 601 PLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL 660
           PLVLRVLEFGGQLQTLFSVRC PHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL
Sbjct: 601 PLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL 660

Query: 661 RRLERGEIEERKNSVSIILCCIRADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLAL 720
           RRLE GEIEERKNSVSII+CCIRADGSCRNYLAEN+NKASLLELIVHESN NSDRCGLAL
Sbjct: 661 RRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLAL 720

Query: 721 LVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRAHPEEQPLAATILLQLDFMED 780
           LVDLLCLSRR RI++LLDGLK+GW+GL IMN+LS+YLQRA PEEQPL ATILLQLDFMED
Sbjct: 721 LVDLLCLSRRARISRLLDGLKDGWNGLSIMNVLSIYLQRARPEEQPLVATILLQLDFMED 780

Query: 781 PLNCSIFREEAIVTIITALNSRLSRENAQESLARALLILGGRFSCSGVPSVENWLLQQAG 840
           PLNC+IFREEAIVTIIT+LN+R+SRE  +E+LARALLILGGRFSC+G P++ENWLLQQAG
Sbjct: 781 PLNCTIFREEAIVTIITSLNARISREKTRENLARALLILGGRFSCTGEPTIENWLLQQAG 840

Query: 841 FKESSGDSFHSKHVYDDFVQSYEEEEDVVNWQLKAATVLFNYGHKSLLSALSTSMASCIQ 900
           FKESSGDSFHSKH+YDDFVQSYEEEE+VVNWQLKAATV FN+GHKSLLSALSTSM SCI 
Sbjct: 841 FKESSGDSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCIP 900

Query: 901 SLAKASLITVSWMSRYLFVVRDEKLCLMAPSILVPTLIKYLSYDKDVEDRVLASYSLLNL 960
           SLAKA L+ VSWMS+YLFVV D+KLCLMAPSILVP LIKYL+YDKDVEDRVLASYSLLNL
Sbjct: 901 SLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKDVEDRVLASYSLLNL 960

Query: 961 SKYTECKHIFRLFDDEALDRLQNLSLVTWTAEELISVIK-RGSTHQHSE 997
           SKYTECKHIFRLFD+EAL+ L+NLSLVTWTAEELIS+++  GSTHQ++E
Sbjct: 961 SKYTECKHIFRLFDEEALEHLRNLSLVTWTAEELISIVEMSGSTHQYTE 980

BLAST of Lag0025331 vs. ExPASy TrEMBL
Match: A0A1S3C8W1 (putative E3 ubiquitin-protein ligase LIN-2 OS=Cucumis melo OX=3656 GN=LOC103498300 PE=4 SV=1)

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 753/872 (86.35%), Postives = 807/872 (92.55%), Query Frame = 0

Query: 137  MESGIFSNLEMGMKSVDRLVVEGHGNEREFRMKASRNSIGLLNMVVTSLSSAKKSMKHGA 196
            MESGI SNLEMGMKSVDRLV EGHGNERE R+KASRNSIGLLNMV+TSL SA K  K+GA
Sbjct: 1    MESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMVITSLDSAIKFTKNGA 60

Query: 197  RSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFSDSPFFARTHLLPELWEHFFLPHLLHL 256
             SHLSACA LYLAIV KIEK EK+SAKHLLQVF DSPFFARTHLLP+LWEHFFLPHLLHL
Sbjct: 61   YSHLSACAHLYLAIVNKIEKKEKISAKHLLQVFCDSPFFARTHLLPDLWEHFFLPHLLHL 120

Query: 257  KVWYNQELEIVSNLESEYKDRKIKALSKVYNEHMDSGTVQFAMYYIQWLKDGARAPPVPV 316
            KVWYNQELE VSN E E+KDRK KAL+KVYN+HMD GTVQFA+YYIQWLKDGARAPPVPV
Sbjct: 121  KVWYNQELEFVSNFECEHKDRKTKALNKVYNDHMDRGTVQFALYYIQWLKDGARAPPVPV 180

Query: 317  VPSPSKSIHGASRSSSDSYFSQSSSNKNLYHAVFGPSLDQQLAELRSGNVVAAKARSSNE 376
            V SPSKSIH ASR SSDSYFSQ SSNKNLYHAVFGPSLDQQLA LRSGN+VAA ARSSNE
Sbjct: 181  VRSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAGLRSGNLVAALARSSNE 240

Query: 377  KEILFADKNYESSVPVHDELGNRRMSSILDYRSHNTDSWRETVKSDYFRFFTCQNVTKEY 436
            KEILF+DK+YE+S  + DE  NRRMSS+LDYRSHNT+SWRETVKSDYFRFFTCQ++TKEY
Sbjct: 241  KEILFSDKHYENSASLQDEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQSITKEY 300

Query: 437  LESTNVIAKNSSVRVEGKSHLLSNDLSRAITTICSSDILSECEIAIRVVTKAWLDAHGHS 496
            LES+NVI KNSSVRVEG++HLLSNDLS+AIT ICSSDILSECEIAIRVVTKAWLDAHG +
Sbjct: 301  LESSNVITKNSSVRVEGRNHLLSNDLSKAITAICSSDILSECEIAIRVVTKAWLDAHGDT 360

Query: 497  AIEVTLSKPPVVEGMLEVLLASDDDEVLELVISVLAELAAKNEVIRQMILNSDPQLQVFL 556
             IEV LSKPPVVEGMLEVLLASDDDE+LELVIS LAELA ++EVIRQMILNSDPQLQVFL
Sbjct: 361  TIEVALSKPPVVEGMLEVLLASDDDEILELVISFLAELAVRSEVIRQMILNSDPQLQVFL 420

Query: 557  KLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAF 616
            KLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAF
Sbjct: 421  KLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAF 480

Query: 617  YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEIEERKNSVSIILCCIRADGSC 676
            YLLDQLLKGFDEDRNLEN RHLI+LGGLSLLLRRLERGEIEERKNSVSII CC++ADGSC
Sbjct: 481  YLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKNSVSIISCCVQADGSC 540

Query: 677  RNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLG 736
            RNYLAENLNKASLLELIVHESNKNS R GLALLVDLLCLSRRTRITKLLDGLKEGWSGLG
Sbjct: 541  RNYLAENLNKASLLELIVHESNKNSGRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLG 600

Query: 737  IMNILSVYLQRAHPEEQPLAATILLQLDFMEDPLNCSIFREEAIVTIITALNSRLSRENA 796
            IMNILSVYLQRA PEEQPL AT+LLQLDFMED LNCSIFREEAIVTIITALN+R+S E  
Sbjct: 601  IMNILSVYLQRALPEEQPLVATMLLQLDFMEDALNCSIFREEAIVTIITALNARISGEKV 660

Query: 797  QESLARALLILGGRFSCSGVPSVENWLLQQAGFKESSGDSFHSKHVYDDFVQSYEEEEDV 856
            Q++LARALLILGGRFSC+G PS ENWLL+ AGF+E+SGDS HSKH+YDD V  YEEEE+V
Sbjct: 661  QDNLARALLILGGRFSCTGEPSTENWLLKLAGFRENSGDSSHSKHLYDDVVLLYEEEEEV 720

Query: 857  VNWQLKAATVLFNYGHKSLLSALSTSMASCIQ-SLAKASLITVSWMSRYLFVVRDEKLCL 916
            VNWQLKAATVLFN+GHKSLLS+LSTSM SC++ SLAKA LIT+SWMSRYLFV+RDEKLCL
Sbjct: 721  VNWQLKAATVLFNHGHKSLLSSLSTSMTSCVRPSLAKACLITLSWMSRYLFVIRDEKLCL 780

Query: 917  MAPSILVPTLIKYLSYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDDEALDRLQNLSLV 976
            MAPSILVP LIKYL++DK VED+VLASYSLLNL KYTECKHIFRLFD+EALD L+NLSLV
Sbjct: 781  MAPSILVPALIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDEEALDHLRNLSLV 840

Query: 977  TWTAEELISVIKRGSTHQHSEQEDSHTQTSTR 1008
            TWTAEELI +I  GS H H+EQE+SHTQ STR
Sbjct: 841  TWTAEELILIITSGSMHPHTEQENSHTQGSTR 872