Lag0025301 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0025301
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	transcription factor bHLH52
Location	chr10: 11021754 .. 11022655 (+)
RNA-Seq Expression	Lag0025301
Synteny	Lag0025301

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCTCTCACATTTTTCTCCAATTTTTACTCCGACCCTTCTTCAATCACTCACCAATTCCACTGTTTCTCCCCTGAATTCTCGCCGGACCTCTTCTTTCTCCCTCCGCCGCAGCTCGTCCCCGACCCCGCCTTCGATTACGTCCCTGCCGTGCTCGACGACTCTGTTTTTTCCCGACCGCCGCTCCGTTCTTCGACGATGCCTCGTCGTTTCTTTTCTCTGATGTCCACCCGTCCTTCTCCGCTCCGGTCGCCGATGAACTCGCTCCCGTCTCGGCCGAATTCTTCCCTTTTGATGAATTTGAGTTGCATTGCCCCAAACGACAGAGGGTCGTGCTGGAGCAGAGTTTTTGTTGCGGCGGTGTCGGTGGTGGTGGAGGAGGCGGAGAAGGCTACTTTCCTCCGCCGGAGTTGTTTTCGGGTCCGTGGGATGGAAGAATGGACAATGCTGGAATGACGAGCAATGGTGGTTGCTTGAAGACTAAACCGTCGCTGTCGCCGTCGTCGTCGAGTAACAACTTGTCGGCACAGACGATTGCGGCCCGGGAACGGCGGAGGAAGATCACGGCGAAGACGCAGGAGCTCGGAGAGCTGGTTCCGGGCGGCAGCAAGATGAACACTGCTGAAATGCTGAATTCTGCTTTCAAATATGTCAAATTCTTGCAAGCCCAAGTCGCCATTCTGCAACTCAAGCAAGAAACAGAGCAAGAACAAGAAGAAGAAGAAGAAACAGAGGATCTTCAGATTCTCGAATCCACAATGATTCAAGAGAAATTATACTCAGAAGAAAAATGTTTGGTTCCAAAAGGGTTCGTCCAAAATCTTGCAAATCTCCCAGAGATTCAATCCCATCCGTCCATTTTCAACTCCATCAATCAGATCCTTCAAACGAGCAGCTAG

mRNA sequence

ATGGCGGCTCTCACATTTTTCTCCAATTTTTACTCCGACCCTTCTTCAATCACTCACCAATTCCACTGTTTCTCCCCTGAATTCTCGCCGGACCTCTTCTTTCTCCCTCCGCCGCAGCTCGTCCCCGACCCCGCCTTCGATTACGTCCCTGCCGTGCTCGACGACTCTGTTTTTTCCCGACCGCCGCTCCGTTCTTCGACGATGCCTCGTCGTTTCTTTTCTCTGATGTCCACCCGTCCTTCTCCGCTCCGGTCGCCGATGAACTCGCTCCCGTCTCGGCCGAATTCTTCCCTTTTGATGAATTTGAGTTGCATTGCCCCAAACGACAGAGGGTCGTGCTGGAGCAGAGTTTTTGTTGCGGCGGTGTCGGTGGTGGTGGAGGAGGCGGAGAAGGCTACTTTCCTCCGCCGGAGTTGTTTTCGGACTAAACCGTCGCTGTCGCCGTCGTCGTCGAGTAACAACTTGTCGGCACAGACGATTGCGGCCCGGGAACGGCGGAGGAAGATCACGGCGAAGACGCAGGAGCTCGGAGAGCTGGTTCCGGGCGGCAGCAAGATGAACACTGCTGAAATGCTGAATTCTGCTTTCAAATATGTCAAATTCTTGCAAGCCCAAGTCGCCATTCTGCAACTCAAGCAAGAAACAGAGCAAGAACAAGAAGAAGAAGAAGAAACAGAGGATCTTCAGATTCTCGAATCCACAATGATTCAAGAGAAATTATACTCAGAAGAAAAATGTTTGGTTCCAAAAGGGTTCGTCCAAAATCTTGCAAATCTCCCAGAGATTCAATCCCATCCGTCCATTTTCAACTCCATCAATCAGATCCTTCAAACGAGCAGCTAG

Coding sequence (CDS)

ATGGCGGCTCTCACATTTTTCTCCAATTTTTACTCCGACCCTTCTTCAATCACTCACCAATTCCACTGTTTCTCCCCTGAATTCTCGCCGGACCTCTTCTTTCTCCCTCCGCCGCAGCTCGTCCCCGACCCCGCCTTCGATTACGTCCCTGCCGTGCTCGACGACTCTGTTTTTTCCCGACCGCCGCTCCGTTCTTCGACGATGCCTCGTCGTTTCTTTTCTCTGATGTCCACCCGTCCTTCTCCGCTCCGGTCGCCGATGAACTCGCTCCCGTCTCGGCCGAATTCTTCCCTTTTGATGAATTTGAGTTGCATTGCCCCAAACGACAGAGGGTCGTGCTGGAGCAGAGTTTTTGTTGCGGCGGTGTCGGTGGTGGTGGAGGAGGCGGAGAAGGCTACTTTCCTCCGCCGGAGTTGTTTTCGGACTAAACCGTCGCTGTCGCCGTCGTCGTCGAGTAACAACTTGTCGGCACAGACGATTGCGGCCCGGGAACGGCGGAGGAAGATCACGGCGAAGACGCAGGAGCTCGGAGAGCTGGTTCCGGGCGGCAGCAAGATGAACACTGCTGAAATGCTGAATTCTGCTTTCAAATATGTCAAATTCTTGCAAGCCCAAGTCGCCATTCTGCAACTCAAGCAAGAAACAGAGCAAGAACAAGAAGAAGAAGAAGAAACAGAGGATCTTCAGATTCTCGAATCCACAATGATTCAAGAGAAATTATACTCAGAAGAAAAATGTTTGGTTCCAAAAGGGTTCGTCCAAAATCTTGCAAATCTCCCAGAGATTCAATCCCATCCGTCCATTTTCAACTCCATCAATCAGATCCTTCAAACGAGCAGCTAG

Protein sequence

MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVFSRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSRPNSSLLMNLSCIAPNDRGSCWSRVFVAAVSVVVEEAEKATFLRRSCFRTKPSLSPSSSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSINQILQTSS

Homology

[Close]

BLAST of Lag0025301 vs. NCBI nr
Match: XP_038894685.1 (transcription factor bHLH52 [Benincasa hispida])

HSP 1 Score: 292.4 bits (747), Expect = 4.3e-75
Identity = 193/315 (61.27%), Postives = 210/315 (66.67%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDP-AFDYVPAVLDDSVF- 60
           MAALTF+SNFYSDPSS  HQFH FSPEFSPDL + PPPQL+ DP AFDYV    DDSVF 
Sbjct: 1   MAALTFYSNFYSDPSSYYHQFHYFSPEFSPDLCYFPPPQLLHDPIAFDYV----DDSVFY 60

Query: 61  --SRPPLRSSTMPRRF---FSLMSTRP-------SPLRSPMNSL----PSRPNSSLLMNL 120
             +  P      P  F   +   ST         SP   P +      P R  + L  + 
Sbjct: 61  PTTAAPFFDDAFPFLFSDAYPYFSTPAVDEFLPVSPEFFPCDEFEFHCPKRQRAVLEQSF 120

Query: 121 SC-----------------IAPNDRGSCWSRVFVAAVSVVVEEAEKATFLRRSCFRTKPS 180
            C                   P    +  S +F +A    ++ AE        C +TKP+
Sbjct: 121 RCGDGGGGGGGNVGGGGGYFPPPPTMTMTSELFSSAWDGRMDNAEMMN--DGGCLKTKPT 180

Query: 181 LSPSSSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQ 240
             P  SSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQ
Sbjct: 181 PVP-LSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQ 240

Query: 241 VAILQLKQETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSH 281
           VAILQLKQETEQEQE+EEETEDL+ILESTMIQEKLYSEEKCLVPKGFVQNLAN PEIQSH
Sbjct: 241 VAILQLKQETEQEQEQEEETEDLRILESTMIQEKLYSEEKCLVPKGFVQNLANFPEIQSH 300

[Close]

BLAST of Lag0025301 vs. NCBI nr
Match: XP_023520229.1 (transcription factor bHLH52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-70
Identity = 179/312 (57.37%), Postives = 203/312 (65.06%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAAL+F+SNF SDPSS  HQFH FSPE SP+LF LPPPQL+PDPAFDY+PA +D SVF  
Sbjct: 1   MAALSFYSNFSSDPSSYHHQFHPFSPEISPELFHLPPPQLLPDPAFDYLPAAVDHSVFFP 60

Query: 61  -SRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSR------------PNSSLLMNLSCI 120
            +  P      P  F  +    P+P       + +                 ++M  S  
Sbjct: 61  TATAPFFDDVSPFVFSDVYPYFPTPAVDSFVPVSAEFFPCDEFEFYCPKRQRVVMEQSFC 120

Query: 121 APNDRGSC----------------WSRVFVAAVSVVVEEAEKATFLRRSCFRTKPSLSPS 180
                G+                 W      A  +   +  K T          P  SPS
Sbjct: 121 YGGGGGNVGGDGGYFPLPEFFSDQWDGRLGNAEMMNKGDCLKGT------LTPAPLPSPS 180

Query: 181 SSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAIL 240
            SSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEML+SAFKYVKFLQAQVAIL
Sbjct: 181 PSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLSSAFKYVKFLQAQVAIL 240

Query: 241 QLKQETEQEQEEEE-ETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSI 281
           Q+KQETEQEQE+E+ ETEDLQILESTMIQEKLY+EEKCLVPKGFVQNL N PEIQS+PSI
Sbjct: 241 QVKQETEQEQEQEQTETEDLQILESTMIQEKLYAEEKCLVPKGFVQNLVNSPEIQSNPSI 300

[Close]

BLAST of Lag0025301 vs. NCBI nr
Match: XP_023001636.1 (transcription factor bHLH52 [Cucurbita maxima])

HSP 1 Score: 273.1 bits (697), Expect = 2.7e-69
Identity = 175/307 (57.00%), Postives = 200/307 (65.15%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAAL+F+SNF SDPSS  HQFH FSPE SP+LF LPPPQL+PDP FDY+PA +D SVF  
Sbjct: 1   MAALSFYSNFSSDPSSYHHQFHPFSPEISPELFHLPPPQLLPDPTFDYLPAAVDHSVFFP 60

Query: 61  -SRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSR------------PNSSLLMNLSCI 120
            +  P      P  F  +    P+P       + +                 ++M  S  
Sbjct: 61  TATAPFFDDVSPFVFSDVYPYFPTPAVDEFVPVSAEFFPCDEFEFYCPKRQRVVMEQSFC 120

Query: 121 APNDRGSCWSRVFVAAVSVVVEE------AEKATFLRRSCFRTKPSL------SPSSSSN 180
                G+         +     +             +  C +  P+L      SPS SSN
Sbjct: 121 YGGGGGNVGGEGGYFPLPEFFSDQWDGRLGNAEMMNKGDCLKGTPTLVPLPLPSPSPSSN 180

Query: 181 NLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQ 240
           NLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEML+SAFKYVKFLQAQVAILQ+KQ
Sbjct: 181 NLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLSSAFKYVKFLQAQVAILQVKQ 240

Query: 241 ETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSIN 281
           ETEQEQ E+ ETEDLQILESTMIQEKLY+EEKCLVPKGFVQNLAN  EIQS+PSI NSIN
Sbjct: 241 ETEQEQ-EQTETEDLQILESTMIQEKLYAEEKCLVPKGFVQNLANSSEIQSNPSILNSIN 300

[Close]

BLAST of Lag0025301 vs. NCBI nr
Match: KAG6583823.1 (Transcription factor basic helix-loop-helix 52, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 269.2 bits (687), Expect = 3.9e-68
Identity = 175/303 (57.76%), Postives = 200/303 (66.01%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAAL+F+SNF SDPSS  HQFH FSPE SP+LF LPPPQL+PDPAFDY+PA +D SVF  
Sbjct: 1   MAALSFYSNFSSDPSSYHHQFHPFSPEISPELFHLPPPQLLPDPAFDYLPAAVDHSVFFP 60

Query: 61  -SRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSR------------PNSSLLMNLSCI 120
            +  P  +   P  F  +    P+P       + +                 ++M  S  
Sbjct: 61  TATAPFFNDVSPFVFSDVYPYLPTPAVDSFVPVSAEFFPCDEFEFYCPKRQRVVMEQSFC 120

Query: 121 APNDRGSCWS--------RVFVAAVSVVVEEAEKATFLRRSCFRTKPSLSPSSSSNNLSA 180
                G+             F       +  AE     +  C   K + +P+ SSNNLSA
Sbjct: 121 YGGGGGNVGGDGGYFPLPEFFSDQWDGRLGNAELMN--KGDCL--KGTRTPAPSSNNLSA 180

Query: 181 QTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQETEQ 240
           QTIAARERRRKITAKTQELGELVPGGSKMNTAEML+SAFKYVKFLQAQVAILQ+KQETEQ
Sbjct: 181 QTIAARERRRKITAKTQELGELVPGGSKMNTAEMLSSAFKYVKFLQAQVAILQVKQETEQ 240

Query: 241 EQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSINQILQ 281
           EQ E  ETEDLQILESTMIQEKLY+EEKCLVPKGFVQNL N PEIQS+PSI NSINQIL 
Sbjct: 241 EQ-EHTETEDLQILESTMIQEKLYAEEKCLVPKGFVQNLVNSPEIQSNPSILNSINQILH 298

[Close]

BLAST of Lag0025301 vs. NCBI nr
Match: XP_022927111.1 (transcription factor bHLH52-like [Cucurbita moschata])

HSP 1 Score: 265.0 bits (676), Expect = 7.3e-67
Identity = 174/305 (57.05%), Postives = 198/305 (64.92%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAAL+F+SNF SDPSS  HQFH FSPE SP+LF LPPPQL+PDPAFDY+ A +D SVF  
Sbjct: 1   MAALSFYSNFSSDPSSYHHQFHPFSPEISPELFHLPPPQLLPDPAFDYLHAAVDHSVFFP 60

Query: 61  -SRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSR------------PNSSLLMNLSCI 120
            +  P      P  F  +    P+P       + +                 ++M  S  
Sbjct: 61  TATAPFFDDVSPFVFSDVYPYFPTPAVDSFVPVSAEFFPCDEFEFYCPKRQRVVMEQSFC 120

Query: 121 APNDRGSCWS--------RVFVAAVSVVVEEAEKATFLRRSCFR--TKPSLSPSSSSNNL 180
                G+             F       +  AE     +  C +    P+  P+ SSNNL
Sbjct: 121 YGGGGGNVGGDGGYFPLPEFFSDQWDGRLGNAELMN--KGDCLKGTLTPAPLPAPSSNNL 180

Query: 181 SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQET 240
           SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEML+SAFKYVKFLQAQVAILQ+KQET
Sbjct: 181 SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLSSAFKYVKFLQAQVAILQVKQET 240

Query: 241 EQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSINQI 281
           EQEQ E  ETEDLQILESTMIQEKLY+EEKCLVPKGFVQNL N PEIQS+PSI NSINQI
Sbjct: 241 EQEQ-ERTETEDLQILESTMIQEKLYAEEKCLVPKGFVQNLVNSPEIQSNPSILNSINQI 300

[Close]

BLAST of Lag0025301 vs. ExPASy Swiss-Prot
Match: Q9SA82 (Transcription factor bHLH52 OS=Arabidopsis thaliana OX=3702 GN=BHLH52 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.2e-23
Identity = 71/143 (49.65%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 138 SCFRTKPSLSPSSSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFK 197
           S F+   +    +    LSAQ+IAAR+RRR+IT KTQELG+L+PG  K NTAEM N+A K
Sbjct: 119 STFKVGWTEQGDTKKRELSAQSIAARKRRRRITEKTQELGKLIPGSQKHNTAEMFNAAAK 178

Query: 198 YVKFLQAQVAILQLKQETEQEQEEEEETEDLQ-ILESTMIQEKLYSEEKCLVPKGFVQNL 257
           YVKFLQAQ+ ILQLKQ   Q  +  +   ++Q +L S  IQEKL +EE C+VP+  VQ L
Sbjct: 179 YVKFLQAQIEILQLKQTKMQTLDSSKVGREMQFLLGSQEIQEKLSTEEVCVVPREMVQVL 238

Query: 258 ANLPEIQSHPSIFNSINQILQTS 280
                I ++P I   IN++L T+
Sbjct: 239 KAEECILTNPKISRDINKLLSTN 261

[Close]

BLAST of Lag0025301 vs. ExPASy Swiss-Prot
Match: Q84RD0 (Transcription factor bHLH53 OS=Arabidopsis thaliana OX=3702 GN=BHLH53 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 7.5e-22
Identity = 67/127 (52.76%), Postives = 85/127 (66.93%), Query Frame = 0


Query: 150 SSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAIL 209
           S    LS+Q+IAAR RRR+I  KT ELG+L+PGG+K+NTAEM  +A KYVKFLQ+QV IL
Sbjct: 160 SKKPTLSSQSIAARGRRRRIAEKTHELGKLIPGGNKLNTAEMFQAAAKYVKFLQSQVGIL 219

Query: 210 QLKQETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIF 269
           QL Q T ++     + E   +LES  IQEKL +EE CLVP   VQ+L     I   P+I 
Sbjct: 220 QLMQ-TTKKGSSNVQMETQYLLESQAIQEKLSTEEVCLVPCEMVQDLTTEETICRTPNIS 279

Query: 270 NSINQIL 277
             IN++L
Sbjct: 280 REINKLL 285

[Close]

BLAST of Lag0025301 vs. ExPASy Swiss-Prot
Match: Q8S3D2 (Transcription factor bHLH87 OS=Arabidopsis thaliana OX=3702 GN=BHLH87 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.0e-08
Identity = 30/53 (56.60%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 158 QTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQ 211
           QT+AAR+RR +I+ K + L  LVPGG+KM+TA ML+ A  Y+KFL+AQV  L+
Sbjct: 280 QTVAARQRRERISEKIRVLQTLVPGGTKMDTASMLDEAANYLKFLRAQVKALE 332

[Close]

BLAST of Lag0025301 vs. ExPASy Swiss-Prot
Match: Q7XAQ6 (Transcription factor LAX PANICLE 1 OS=Oryza sativa subsp. japonica OX=39947 GN=LAX1 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.8e-07
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 0


Query: 158 QTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQ 211
           Q++AARERR +I+ + + L  LVPGGSKM+T  ML  A  YVKFL+AQV + Q
Sbjct: 45  QSVAARERRHRISDRFRVLRSLVPGGSKMDTVSMLEQAIHYVKFLKAQVTLHQ 97

[Close]

BLAST of Lag0025301 vs. ExPASy Swiss-Prot
Match: O81313 (Transcription factor IND OS=Arabidopsis thaliana OX=3702 GN=IND PE=1 SV=3)

HSP 1 Score: 57.4 bits (137), Expect = 3.0e-07
Identity = 32/71 (45.07%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 144 PSLSPSSSSNNL----SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYV 203
           P+  P  +  N+      QT+ AR RR +I+ K + L  +VPGG+KM+TA ML+ A +Y 
Sbjct: 105 PATVPKPNRRNVRISDDPQTVVARRRRERISEKIRILKRIVPGGAKMDTASMLDEAIRYT 164

Query: 204 KFLQAQVAILQ 211
           KFL+ QV ILQ
Sbjct: 165 KFLKRQVRILQ 175

[Close]

BLAST of Lag0025301 vs. ExPASy TrEMBL
Match: A0A6J1KJ69 (transcription factor bHLH52 OS=Cucurbita maxima OX=3661 GN=LOC111495708 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 175/307 (57.00%), Postives = 200/307 (65.15%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAAL+F+SNF SDPSS  HQFH FSPE SP+LF LPPPQL+PDP FDY+PA +D SVF  
Sbjct: 1   MAALSFYSNFSSDPSSYHHQFHPFSPEISPELFHLPPPQLLPDPTFDYLPAAVDHSVFFP 60

Query: 61  -SRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSR------------PNSSLLMNLSCI 120
            +  P      P  F  +    P+P       + +                 ++M  S  
Sbjct: 61  TATAPFFDDVSPFVFSDVYPYFPTPAVDEFVPVSAEFFPCDEFEFYCPKRQRVVMEQSFC 120

Query: 121 APNDRGSCWSRVFVAAVSVVVEE------AEKATFLRRSCFRTKPSL------SPSSSSN 180
                G+         +     +             +  C +  P+L      SPS SSN
Sbjct: 121 YGGGGGNVGGEGGYFPLPEFFSDQWDGRLGNAEMMNKGDCLKGTPTLVPLPLPSPSPSSN 180

Query: 181 NLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQ 240
           NLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEML+SAFKYVKFLQAQVAILQ+KQ
Sbjct: 181 NLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLSSAFKYVKFLQAQVAILQVKQ 240

Query: 241 ETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSIN 281
           ETEQEQ E+ ETEDLQILESTMIQEKLY+EEKCLVPKGFVQNLAN  EIQS+PSI NSIN
Sbjct: 241 ETEQEQ-EQTETEDLQILESTMIQEKLYAEEKCLVPKGFVQNLANSSEIQSNPSILNSIN 300

[Close]

BLAST of Lag0025301 vs. ExPASy TrEMBL
Match: A0A6J1EGS4 (transcription factor bHLH52-like OS=Cucurbita moschata OX=3662 GN=LOC111434050 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 3.5e-67
Identity = 174/305 (57.05%), Postives = 198/305 (64.92%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAAL+F+SNF SDPSS  HQFH FSPE SP+LF LPPPQL+PDPAFDY+ A +D SVF  
Sbjct: 1   MAALSFYSNFSSDPSSYHHQFHPFSPEISPELFHLPPPQLLPDPAFDYLHAAVDHSVFFP 60

Query: 61  -SRPPLRSSTMPRRFFSLMSTRPSPLRSPMNSLPSR------------PNSSLLMNLSCI 120
            +  P      P  F  +    P+P       + +                 ++M  S  
Sbjct: 61  TATAPFFDDVSPFVFSDVYPYFPTPAVDSFVPVSAEFFPCDEFEFYCPKRQRVVMEQSFC 120

Query: 121 APNDRGSCWS--------RVFVAAVSVVVEEAEKATFLRRSCFR--TKPSLSPSSSSNNL 180
                G+             F       +  AE     +  C +    P+  P+ SSNNL
Sbjct: 121 YGGGGGNVGGDGGYFPLPEFFSDQWDGRLGNAELMN--KGDCLKGTLTPAPLPAPSSNNL 180

Query: 181 SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQET 240
           SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEML+SAFKYVKFLQAQVAILQ+KQET
Sbjct: 181 SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLSSAFKYVKFLQAQVAILQVKQET 240

Query: 241 EQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSINQI 281
           EQEQ E  ETEDLQILESTMIQEKLY+EEKCLVPKGFVQNL N PEIQS+PSI NSINQI
Sbjct: 241 EQEQ-ERTETEDLQILESTMIQEKLYAEEKCLVPKGFVQNLVNSPEIQSNPSILNSINQI 300

[Close]

BLAST of Lag0025301 vs. ExPASy TrEMBL
Match: A0A6J1GUV4 (transcription factor bHLH52-like OS=Cucurbita moschata OX=3662 GN=LOC111457139 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.5e-65
Identity = 174/287 (60.63%), Postives = 190/287 (66.20%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAALTFFSNFYSDPSSI  QF  FSPE SPDLFFLPPP L      DYVPA+ DDSVF  
Sbjct: 1   MAALTFFSNFYSDPSSINPQFPYFSPEMSPDLFFLPPPHL------DYVPAMPDDSVFIP 60

Query: 61  SRPPLRSSTMPRRF---FSLMSTRPSPLRSPMNSLPSRPNSSLLMNLSC---IAPNDRGS 120
           +  P      P  F   +   S    P         S+    +L    C   +  N  G 
Sbjct: 61  TVAPFFDDASPFLFSDVYPYFSAGFFPF-DEFEFHYSKRQRIVLEQSFCYGGVGGNVGGG 120

Query: 121 CWSRVFVAAVSVVVEEAEKATFLRRSCFRTKPSLSPSSSSNNLSAQTIAARERRRKITAK 180
                +  +  +             +C +TK  +SP   SN LS QTIAARERRRKITAK
Sbjct: 121 GGGGGYFPSGLLDGRVDNVEMMNNGNCSKTKQLVSP---SNTLSVQTIAARERRRKITAK 180

Query: 181 TQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQETEQEQEEEEETEDLQILE 240
           TQELGEL+PGG KMNTAEMLNSAFKYVKFLQAQVAILQLKQETE E  EE+ETEDLQILE
Sbjct: 181 TQELGELIPGGVKMNTAEMLNSAFKYVKFLQAQVAILQLKQETEHEL-EEQETEDLQILE 240

Query: 241 STMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSINQILQTS 280
           S MIQEKLYSEE+CLVPKGFVQNLAN PEIQSHPSI NSINQIL+TS
Sbjct: 241 SAMIQEKLYSEEQCLVPKGFVQNLANFPEIQSHPSISNSINQILKTS 276

[Close]

BLAST of Lag0025301 vs. ExPASy TrEMBL
Match: A0A0A0LY56 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542450 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 9.7e-65
Identity = 178/320 (55.62%), Postives = 205/320 (64.06%), Query Frame = 0


Query: 1   MAALTFFSNFYS-DPSSITHQFHCFSPEFSPDLFFLPPPQLVPDP-AFDYVPAVLDDSVF 60
           MAALT++SNFYS DPS   H FH FSPEFSPDL ++PPPQL+  P AFDYV    DDS+F
Sbjct: 1   MAALTYYSNFYSPDPSPYYHHFHYFSPEFSPDLSYVPPPQLLNHPAAFDYV----DDSLF 60

Query: 61  -----------SRPPLRSSTMP------RRFFSLMSTRPSPLRSPMNSLPSRPNSSLLMN 120
                      + P L S T P         F  +S++  P        P R  +    +
Sbjct: 61  YPTADTLLFDDALPFLFSDTYPCFSAPSVDEFLPVSSQFFPFDEFEFHCPKRQRAVFEHS 120

Query: 121 LSC---IAPNDRGSCWS------------------RVFVAAVSVVVEEAEKATFLRRSCF 180
             C   +   + G   S                   VF       ++ AE    +R  C 
Sbjct: 121 FCCGGGVGDGNVGGGGSGAGAGFFPSPPPPPPPLAEVFSGPWDSRMDNAE----MRNDCL 180

Query: 181 RTKPSLSPSSSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVK 240
           +++PS +P SS NNLSAQTIAARERRRKIT KTQELGELVPGGSKMNTAEMLNSAFKYVK
Sbjct: 181 KSQPSPAP-SSHNNLSAQTIAARERRRKITVKTQELGELVPGGSKMNTAEMLNSAFKYVK 240

Query: 241 FLQAQVAILQLKQETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLP 281
           FLQAQVAILQLKQETEQ   E +ETE+L+ILESTMIQEKLYSEEKCLVPKGF+QNLA+ P
Sbjct: 241 FLQAQVAILQLKQETEQ---EGQETENLEILESTMIQEKLYSEEKCLVPKGFIQNLADFP 300

[Close]

BLAST of Lag0025301 vs. ExPASy TrEMBL
Match: A0A6J1K695 (uncharacterized protein LOC111490459 OS=Cucurbita maxima OX=3661 GN=LOC111490459 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 5.3e-63
Identity = 172/290 (59.31%), Postives = 190/290 (65.52%), Query Frame = 0


Query: 1   MAALTFFSNFYSDPSSITHQFHCFSPEFSPDLFFLPPPQLVPDPAFDYVPAVLDDSVF-- 60
           MAALTFFSNFYSDPSSI  QF  FSPE SPDLFFLPPP L      DYVPA+ DDSV   
Sbjct: 1   MAALTFFSNFYSDPSSINPQFPYFSPEMSPDLFFLPPPHL------DYVPAMPDDSVLFP 60

Query: 61  SRPPLRSSTMPRRF---FSLMSTRPSPLRSPMNSLPSRPNSSLLMNLSC---IAPNDRGS 120
           +  P      P  F   +   S    P         S+    +L    C   +  N  G 
Sbjct: 61  TVAPFFDDASPFLFSDVYPYFSAEIFPF-DEFEFHYSKRQRIVLEQSFCYGGVGGNVGGG 120

Query: 121 C--WSRVFVAAVSVVVEEAEKATFLRRS-CFRTKPSLSPSSSSNNLSAQTIAARERRRKI 180
           C          + ++    + A  +  S C +TK      SSSN  S QTIAARERRR+I
Sbjct: 121 CGGGGGGGYFPLGLLDGRMDNAEMMNNSNCSKTK---QLPSSSNASSVQTIAARERRRRI 180

Query: 181 TAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQLKQETEQEQEEEEETEDLQ 240
           TAKTQELGEL+PGG KMNTAEMLNSAFKYVKFLQAQVAILQLKQETE E  EE+ETEDLQ
Sbjct: 181 TAKTQELGELIPGGVKMNTAEMLNSAFKYVKFLQAQVAILQLKQETEHEL-EEQETEDLQ 240

Query: 241 ILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIFNSINQILQTS 280
           IL+S MIQEKLYSEE+CLVPKGFVQNLAN PEIQSHP I NSINQIL+TS
Sbjct: 241 ILDSAMIQEKLYSEEQCLVPKGFVQNLANFPEIQSHPFISNSINQILKTS 279

[Close]

BLAST of Lag0025301 vs. TAIR 10
Match: AT1G30670.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.7e-24
Identity = 71/143 (49.65%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 138 SCFRTKPSLSPSSSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFK 197
           S F+   +    +    LSAQ+IAAR+RRR+IT KTQELG+L+PG  K NTAEM N+A K
Sbjct: 119 STFKVGWTEQGDTKKRELSAQSIAARKRRRRITEKTQELGKLIPGSQKHNTAEMFNAAAK 178

Query: 198 YVKFLQAQVAILQLKQETEQEQEEEEETEDLQ-ILESTMIQEKLYSEEKCLVPKGFVQNL 257
           YVKFLQAQ+ ILQLKQ   Q  +  +   ++Q +L S  IQEKL +EE C+VP+  VQ L
Sbjct: 179 YVKFLQAQIEILQLKQTKMQTLDSSKVGREMQFLLGSQEIQEKLSTEEVCVVPREMVQVL 238

Query: 258 ANLPEIQSHPSIFNSINQILQTS 280
                I ++P I   IN++L T+
Sbjct: 239 KAEECILTNPKISRDINKLLSTN 261

[Close]

BLAST of Lag0025301 vs. TAIR 10
Match: AT2G34820.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 5.3e-23
Identity = 67/127 (52.76%), Postives = 85/127 (66.93%), Query Frame = 0


Query: 150 SSSNNLSAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAIL 209
           S    LS+Q+IAAR RRR+I  KT ELG+L+PGG+K+NTAEM  +A KYVKFLQ+QV IL
Sbjct: 160 SKKPTLSSQSIAARGRRRRIAEKTHELGKLIPGGNKLNTAEMFQAAAKYVKFLQSQVGIL 219

Query: 210 QLKQETEQEQEEEEETEDLQILESTMIQEKLYSEEKCLVPKGFVQNLANLPEIQSHPSIF 269
           QL Q T ++     + E   +LES  IQEKL +EE CLVP   VQ+L     I   P+I 
Sbjct: 220 QLMQ-TTKKGSSNVQMETQYLLESQAIQEKLSTEEVCLVPCEMVQDLTTEETICRTPNIS 279

Query: 270 NSINQIL 277
             IN++L
Sbjct: 280 REINKLL 285

[Close]

BLAST of Lag0025301 vs. TAIR 10
Match: AT3G21330.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.7e-09
Identity = 30/53 (56.60%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 158 QTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAILQ 211
           QT+AAR+RR +I+ K + L  LVPGG+KM+TA ML+ A  Y+KFL+AQV  L+
Sbjct: 280 QTVAARQRRERISEKIRVLQTLVPGGTKMDTASMLDEAANYLKFLRAQVKALE 332

[Close]

BLAST of Lag0025301 vs. TAIR 10
Match: AT4G00120.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 2.2e-08
Identity = 32/71 (45.07%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 144 PSLSPSSSSNNL----SAQTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYV 203
           P+  P  +  N+      QT+ AR RR +I+ K + L  +VPGG+KM+TA ML+ A +Y 
Sbjct: 105 PATVPKPNRRNVRISDDPQTVVARRRRERISEKIRILKRIVPGGAKMDTASMLDEAIRYT 164

Query: 204 KFLQAQVAILQ 211
           KFL+ QV ILQ
Sbjct: 165 KFLKRQVRILQ 175

[Close]

BLAST of Lag0025301 vs. TAIR 10
Match: AT5G09750.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-08
Identity = 27/52 (51.92%), Postives = 40/52 (76.92%), Query Frame = 0


Query: 158 QTIAARERRRKITAKTQELGELVPGGSKMNTAEMLNSAFKYVKFLQAQVAIL 210
           Q++AAR RR +I+ + + L  LVPGG+KM+TA ML+ A +YVKFL+ Q+ +L
Sbjct: 130 QSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIRYVKFLKRQIRLL 181

The following BLAST results are available for this feature:

BLAST of Lag0025301 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894685.1	4.3e-75	61.27	transcription factor bHLH52 [Benincasa hispida]	[more]
XP_023520229.1	1.9e-70	57.37	transcription factor bHLH52-like [Cucurbita pepo subsp. pepo]	[more]
XP_023001636.1	2.7e-69	57.00	transcription factor bHLH52 [Cucurbita maxima]	[more]
KAG6583823.1	3.9e-68	57.76	Transcription factor basic helix-loop-helix 52, partial [Cucurbita argyrosperma ...	[more]
XP_022927111.1	7.3e-67	57.05	transcription factor bHLH52-like [Cucurbita moschata]	[more]

BLAST of Lag0025301 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA82	5.2e-23	49.65	Transcription factor bHLH52 OS=Arabidopsis thaliana OX=3702 GN=BHLH52 PE=2 SV=1	[more]
Q84RD0	7.5e-22	52.76	Transcription factor bHLH53 OS=Arabidopsis thaliana OX=3702 GN=BHLH53 PE=2 SV=1	[more]
Q8S3D2	8.0e-08	56.60	Transcription factor bHLH87 OS=Arabidopsis thaliana OX=3702 GN=BHLH87 PE=1 SV=1	[more]
Q7XAQ6	1.8e-07	56.60	Transcription factor LAX PANICLE 1 OS=Oryza sativa subsp. japonica OX=39947 GN=L...	[more]
O81313	3.0e-07	45.07	Transcription factor IND OS=Arabidopsis thaliana OX=3702 GN=IND PE=1 SV=3	[more]

BLAST of Lag0025301 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KJ69	1.3e-69	57.00	transcription factor bHLH52 OS=Cucurbita maxima OX=3661 GN=LOC111495708 PE=4 SV=...	[more]
A0A6J1EGS4	3.5e-67	57.05	transcription factor bHLH52-like OS=Cucurbita moschata OX=3662 GN=LOC111434050 P...	[more]
A0A6J1GUV4	1.5e-65	60.63	transcription factor bHLH52-like OS=Cucurbita moschata OX=3662 GN=LOC111457139 P...	[more]
A0A0A0LY56	9.7e-65	55.63	BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542450 PE=4 S...	[more]
A0A6J1K695	5.3e-63	59.31	uncharacterized protein LOC111490459 OS=Cucurbita maxima OX=3661 GN=LOC111490459...	[more]

BLAST of Lag0025301 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30670.1	3.7e-24	49.65	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G34820.1	5.3e-23	52.76	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G21330.1	5.7e-09	56.60	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G00120.1	2.2e-08	45.07	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G09750.1	2.8e-08	51.92	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 199..234
None	No IPR available	PANTHER	PTHR16223:SF49	TRANSCRIPTION FACTOR BHLH52-RELATED	coord: 24..271
None	No IPR available	PANTHER	PTHR16223	TRANSCRIPTION FACTOR BHLH83-RELATED	coord: 24..271
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 159..208 e-value: 1.8E-9 score: 47.4
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 160..203 e-value: 7.8E-6 score: 25.8
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 153..202 score: 12.302888
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 155..229 e-value: 1.2E-10 score: 43.3
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 151..214

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0025301.1	Lag0025301.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding