Lag0025261 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0025261 vs. NCBI nr
Match: XP_023000935.1 (berberine bridge enzyme-like 27 [Cucurbita maxima])

HSP 1 Score: 853.6 bits (2204), Expect = 9.4e-244
Identity = 416/540 (77.04%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MKSS L LL    IVL SSSL  A SS++++DF+ CLLHHSPD++SITKLVYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 VAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VAY+PFIV+D+INL  V VD EK+TAWVQSGA+LGELYYRIAEKSRTLAFPAG+CPTVGV
Sbjct: 121 VAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIV 240
           GG  SGGGYG+++RKYGLAADNV+DAYLVDANG+F DRE MGE LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQG 300
           VAWKVKLVPVPATVT+C+T+++L + AI LI +WQ+VADKLDENLFLGI L G      G
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------G 300

Query: 301 GKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVL 360
           GKTNP+A+FFSLFLGKA++LV IL   FPELGLSKEDCIETSWIE+ALIAANGFQTGEVL
Sbjct: 301 GKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGEVL 360

Query: 361 EALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRM 420
           EALLNRSALIPPI +G  K KSDYVKEP+   AIEG+W R KSQ++E   L  +PYGG+M
Sbjct: 361 EALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGKM 420

Query: 421 SQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAY 480
           S+ISE+E P+ HRAG  Y+I Y++GW EQ V AEKRHLNWIRELYSYM PFVSKSPRA Y
Sbjct: 421 SEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRAVY 480

Query: 481 VNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTLT 540
           VN RDLDIGSN+  G TSY++ARVWGLKYFGNNFDRLV VKTEVD  +FFRHEQSIPTL+
Sbjct: 481 VNCRDLDIGSNDMYGRTSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQSIPTLS 534

BLAST of Lag0025261 vs. NCBI nr
Match: XP_022927075.1 (berberine bridge enzyme-like 27 [Cucurbita moschata])

HSP 1 Score: 850.1 bits (2195), Expect = 1.0e-242
Identity = 414/540 (76.67%), Postives = 468/540 (86.67%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MKSS L LL    IVL SSSL  A SS++++DF+ CLLHHSPD++SITKLVYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIPHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 VAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VA++PFIV+D+INL SV VD EK+TAWVQSGA+LGELYYRIAEKSRTL FPAG+CPTVGV
Sbjct: 121 VAHVPFIVVDLINLSSVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLTFPAGLCPTVGV 180

Query: 181 GGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIV 240
           GG  SGGGYG+++RKYGLAADNV+DAYLVD NG+F DRE MGE LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDGNGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQG 300
           VAWKVKLVPVPATVT+C+T+++L + AI LI +WQ+VADKLDENLFLGI L G      G
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAISLIDKWQYVADKLDENLFLGIILAG------G 300

Query: 301 GKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVL 360
           GKTNP+A+FFSLFLGKA++LV IL   FPELGL K DCIETSWIE+ALIAANGFQTGEVL
Sbjct: 301 GKTNPLAIFFSLFLGKADELVTILNNAFPELGLPKGDCIETSWIEAALIAANGFQTGEVL 360

Query: 361 EALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRM 420
           EALLNRSALIPPI +G  K KSDYVKEP+   AIEG+W R KSQ++E   L  +PYGG+M
Sbjct: 361 EALLNRSALIPPINSGKSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGKM 420

Query: 421 SQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAY 480
           S+ISE+E P+ HRAG  Y+I Y++GW EQ V AEKRHLNWIRELYSYMAPFVSKSPRAAY
Sbjct: 421 SEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTLT 540
           VN RDLDIGSN+  G TSYQ+ARVWGLKYFGNNFDRL  VKTEVD  +FFRHEQSIPTL+
Sbjct: 481 VNCRDLDIGSNDMYGRTSYQRARVWGLKYFGNNFDRLAHVKTEVDPYDFFRHEQSIPTLS 534

BLAST of Lag0025261 vs. NCBI nr
Match: XP_023520064.1 (berberine bridge enzyme-like 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.9 bits (2184), Expect = 2.0e-241
Identity = 409/540 (75.74%), Postives = 471/540 (87.22%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MKSS L LL    IVL SSSL  A SS++++DF+ CLLHHSPD++SITKLVYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFIQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSHGLQIRIRSGGHD EGLS+
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIPHIQAAIICSKSHGLQIRIRSGGHDLEGLSF 120

Query: 121 VAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VA++PFIV+D+INL SV VD EK+TAWVQSGA+LGELYYRIAEKSRTLAFPAG+CPTVGV
Sbjct: 121 VAHVPFIVVDLINLSSVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIV 240
           GG  SGGGYG+++RKYGLAADNV+DAYLVD NG+F DRE MGE LFW IRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDGNGEFFDRERMGEELFWGIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQG 300
           VAWKVKLVPVPATVT+C+T+++L + AI LI +WQ+VADKLDENLFLGI L G      G
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------G 300

Query: 301 GKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVL 360
           GKTNP+A+FFSLFLGKA++LV IL   FPELGLSKE+CIETSWIE+ALIAANGFQTGEVL
Sbjct: 301 GKTNPLAIFFSLFLGKADELVTILNNAFPELGLSKENCIETSWIEAALIAANGFQTGEVL 360

Query: 361 EALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRM 420
           EALLNRSALIPPI +G  K KSDYVK+P+   AIEG+W R KSQ++E   L  +PYGG+M
Sbjct: 361 EALLNRSALIPPINSGRSKTKSDYVKQPMTRTAIEGMWNRSKSQEIERASLVFIPYGGKM 420

Query: 421 SQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAY 480
           S+IS++E P+ HRAG  Y+I Y++GW EQ V+AEKRHLNW+RELYSYMAPFVS SPRAAY
Sbjct: 421 SEISDSEIPFPHRAGNRYEIDYIVGWDEQGVEAEKRHLNWMRELYSYMAPFVSNSPRAAY 480

Query: 481 VNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTLT 540
           VN RDLDIGSN+  G TSY++ARVWGLKYFGNNFDRLV VKT+VD  +FFRHEQSIPTL+
Sbjct: 481 VNCRDLDIGSNDMYGRTSYERARVWGLKYFGNNFDRLVHVKTKVDPYDFFRHEQSIPTLS 534

BLAST of Lag0025261 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 839.3 bits (2167), Expect = 1.8e-239
Identity = 420/545 (77.06%), Postives = 468/545 (85.87%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVL-SSSSLCTATSSD--KHQDFVGCLLHHSPDSYSITKLVYTPINS 60
           MK S LI L  A I+L SSSSL +ATSS   K+++F+ CLLHHSP++YSIT LVY P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SYSSVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEG 120
           SYSS+LNFSIRNLRFSTP  PKPL+IITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPT 180
           LSYVAY PFIV+D+INL SV VD E+STAWVQSGATLGELYYRIAEKSRTLAFPAG CPT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSF 240
           VGVGG+ SGGGYG +LRKYGLAADNVIDAYLVDANG+FHDRESMGE LFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTS 300
           GIVVAWKVKLVPVPATVTIC+  RT +E AIKLI +WQ+VA+KLDE+LFL I LTGG  S
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 TQGGKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQ-T 360
            QGGK NP ALFF+LFLGKA++L+ ILK  FPEL L+KEDC ETSWIESA+  ANG Q  
Sbjct: 301 AQGGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQID 360

Query: 361 GEVLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPY 420
            + LEA LNR+    P+  GS KIKSDYVKEPI +AAIEGIWQRLKSQD+E   L L+PY
Sbjct: 361 DQPLEAFLNRT----PLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPY 420

Query: 421 GGRMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSP 480
           GGRMSQISE+E P+ HR G LYKIGY+IGW+EQ VDAEKRHLNWIRE+YSYM PFVSKSP
Sbjct: 421 GGRMSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSP 480

Query: 481 RAAYVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSI 540
           RAAYVNYRDLDIGSN+K G+TSY++A +WG KYFGNNF+RLV VK EVD  +FFRHEQSI
Sbjct: 481 RAAYVNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSI 540

Query: 541 PTLTH 542
           P+  H
Sbjct: 541 PSFPH 541

BLAST of Lag0025261 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-236
Identity = 415/542 (76.57%), Postives = 472/542 (87.08%), Query Frame = 0

Query: 3   SSSLILLPFAL--IVLSSSSLCTATSSDKHQDFVGCLLHHSPDS-YSITKLVYTPINSSY 62
           SS LI L F+L  I LSSSSL  A S DKH+ F+ CLL HSPDS YSI+K+VYTP+NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLS 122
           SSVL+FSIRNLRFST  TPKPL IITP ++SH+QAAV+CS++HGLQIR RSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVG 182
            V++LPFI+ID+INLRS+ +DA  +TAWVQSGATLGELYY IAEKSRTLAFPAG CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGI 242
           +GG+ SGGGYGLMLRK+GLAADNVIDAYLVDANGKF DRE MGE LFWAIRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQ 302
           VVAWKVKLVPVPATVT C   RTLEE AIKLIHRWQ+VA+KLDENLFLGI L GGN + Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 GG-KTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGE 362
           GG KTNP A FFSLFLGKA++  AIL TTFPELGL+KE+C+E+SWIESAL++A GF+ G+
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGD 363

Query: 363 VLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGG 422
            LEALLNR+    P+T GS KIKSDYVKEPI+EA IEGIW+RLKS+DV A QL  VPYGG
Sbjct: 364 SLEALLNRT----PLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 423

Query: 423 RMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRA 482
           RMSQISE+E P+SHRAG LYKIGY++ WQ+Q+VD EKRHL WIRELYSYM PFVSKSPR+
Sbjct: 424 RMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRS 483

Query: 483 AYVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPT 541
           AYVNYRDLDIGSNNK G+TS+++A VWG KYFGNNF+RLV VKT+VD  +FFRHEQSIPT
Sbjct: 484 AYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 540

BLAST of Lag0025261 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 5.3e-149
Identity = 266/541 (49.17%), Postives = 376/541 (69.50%), Query Frame = 0

Query: 2   KSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSS--Y 61
           ++++L L+   LI +  S      SS   QDFV CL+ +S  S+ IT   ++P  ++  +
Sbjct: 8   RNATLFLVTLLLISVPLS------SSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLF 67

Query: 62  SSVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLS 121
              L  + +NLR+ TP  PKP+ I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS
Sbjct: 68  KEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLS 127

Query: 122 YVA--YLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPT 181
           +VA    PF+++D+  LR V VD + ++AW  +GAT+GE+YYRI EKS+T  FPAG+C +
Sbjct: 128 FVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSS 187

Query: 182 VGVGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSF 241
           +G+GG+L GG YG M+RK+GL ADNV+DA +VDANG+  DR +MGE +FWAIRGGGGGSF
Sbjct: 188 LGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSF 247

Query: 242 GIVVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTS 301
           G+++AWK+KLVPVPATVT+ + T+TLE+D  K++++W+ +ADKLD++LF+ + ++  + +
Sbjct: 248 GVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKT 307

Query: 302 TQGGKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTG 361
           T+ G       + + FLG +N L+ +++ +FPELGL+K+DC E SWI+S +  A GF   
Sbjct: 308 TKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNS 367

Query: 362 EVLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYG 421
              EALL   +L         K KSD+VKEPI    +EG+W+R   +D   T     PYG
Sbjct: 368 AAPEALLAGKSLF----KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYG 427

Query: 422 GRMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPR 481
           G MS+ISE+E P+ HR G L+KI ++  WQ+  V +E+RH+ WIRE+YSYM  +VSK+PR
Sbjct: 428 GMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPR 487

Query: 482 AAYVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIP 539
            AYVNYRDLD+G+N   GET    AR WG KY+  NF+RLVK+K E D +NFFRHEQS+P
Sbjct: 488 QAYVNYRDLDLGTN--EGET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 529

BLAST of Lag0025261 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 262/519 (50.48%), Postives = 357/519 (68.79%), Query Frame = 0

Query: 27  SDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLVIITP 86
           ++  ++F+ C   H P++ +  KLVYT  +  Y S+LN +I+NLRF +  TPKPLVI+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 87  SNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYLPFIVIDMINLRSVIVDAEKSTA 146
           SN SHIQA ++CSK  GLQIR RSGGHD EG+SY++ +PF+V+D+ N+ S+ +D    TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 147 WVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGYLSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY I EK+  L+FP G CPTVGVGG+ SGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 YLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTTRTLE-E 266
           +LV+ +GK  DR+SMGE LFWAIRGGGG +FGI+ AWK+KLV VP+  TI S  + +E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 267 DAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQGG--KTNPVALFFSLFLGKANDLVAI 326
             +KL ++WQ +A K D++L L       N +   G  KT     F S+F G  + LV +
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 327

Query: 327 LKTTFPELGLSKEDCIETSWIESALIAAN--GFQTGEV-LEALLNRSALIPPITTGSGKI 386
           +  +FPELG+ K DC E SWI++ +  +    F T     E LL+RSA        +  I
Sbjct: 328 MNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSA----GKKTAFSI 387

Query: 387 KSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRMSQISETETPYSHRAGILYKI 446
           K DYVK+PI E A+  I ++L  +DV A    L PYGG M +ISE+  P+ HRAGI+Y++
Sbjct: 388 KLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYEL 447

Query: 447 GYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAYVNYRDLDIGSNNKNGETSYQ 506
            Y   W++Q  +  ++H+NW+R +Y++  P+VS++PR AY+NYRDLD+G  N     +Y 
Sbjct: 448 WYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYT 507

Query: 507 QARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTL 540
           QAR+WG KYFG NF+RLVKVKT+VD  NFFR+EQSIP L
Sbjct: 508 QARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Lag0025261 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-147
Identity = 262/519 (50.48%), Postives = 362/519 (69.75%), Query Frame = 0

Query: 27  SDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYSSVLNFSIRNLRFSTPKTPKPLVIITP 86
           ++  ++F+ C   + P++ +  KLVYT  N  Y SVLN +I NLRF++  TPKPLVI+TP
Sbjct: 28  ANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTP 87

Query: 87  SNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYLPFIVIDMINLRSVIVDAEKSTA 146
           S++SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF+++D+ N+RS+ +D    TA
Sbjct: 88  SHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTA 147

Query: 147 WVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGYLSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY + EK+  L+  AG CPTV  GG+  GGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDA 207

Query: 207 YLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTTRTLE-E 266
           +LV+ +GK  DR+SMGE LFWA+RGGG  SFGI+VAWK++LV VP + T+ S  + +E  
Sbjct: 208 HLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIH 267

Query: 267 DAIKLIHRWQFVADKLDENLFLGI-TLTGGNTSTQG-GKTNPVALFFSLFLGKANDLVAI 326
           + +KL+++WQ +A K D++L L    +T   T  QG  KT     F S+FLG  + LV +
Sbjct: 268 ELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDL 327

Query: 327 LKTTFPELGLSKEDCIETSWIESALIAAN--GFQTGEV-LEALLNRSALIPPITTGSGKI 386
           +  +FPELG+ K DC + SWI++ +  +    + T     E LL+RSA       G+ KI
Sbjct: 328 MNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSA----GQNGAFKI 387

Query: 387 KSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRMSQISETETPYSHRAGILYKI 446
           K DYVK+PI E+    I ++L  +D+ A    L PYGG M +ISE+  P+ HRAGILY++
Sbjct: 388 KLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYEL 447

Query: 447 GYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAYVNYRDLDIGSNNKNGETSYQ 506
            Y+  W++Q  +  ++HLNWIR +Y++M P+VSK+PR AY+NYRDLDIG N+     +Y 
Sbjct: 448 WYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYT 507

Query: 507 QARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTL 540
           QAR+WG KYFG NFDRLVKVKT VD  NFFR+EQSIP L
Sbjct: 508 QARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Lag0025261 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.7e-147
Identity = 265/538 (49.26%), Postives = 372/538 (69.14%), Query Frame = 0

Query: 5   SLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYSSVLN 64
           S  L    L  L+ S L +A  S+ H+DF+ CL +   D+    K+++T  +SS+ S+L+
Sbjct: 4   SSFLFTILLFSLNISPLVSAHGSN-HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILD 63

Query: 65  FSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAY- 124
            SI+N RFS  +TPKP+ IITP   S +Q  + C++ HG+ +R RS GH +EGLSY+AY 
Sbjct: 64  SSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYN 123

Query: 125 LPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGY 184
            PF VID+ NLRS+ +D +  T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGG 
Sbjct: 124 KPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQ 183

Query: 185 LSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVAW 244
            SGGGYG +LRKYGLAADN+IDA +VDA+G+  DR++MGE  FWAIRGGGG SFG++++W
Sbjct: 184 FSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSW 243

Query: 245 KVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQGGKT 304
           KVKLV VP+T+T+    +T +++A+++I +WQ+ ADK+ ++LF+  TL   N      K 
Sbjct: 244 KVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN------KN 303

Query: 305 NPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVLEAL 364
              ALF  L++G  N+L+A+++  FPELGL KE C E SWIES L  A+ F  GE L  L
Sbjct: 304 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVL 363

Query: 365 LNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRMSQI 424
            NR       T+ S K K D+V+EPI EAAI+ IW+RL++ +    ++ L P+GG+MS++
Sbjct: 364 TNRER-----TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEM 423

Query: 425 SETETPYSHRAGILYKIGYVIGWQEQSVDAEK----RHLNWIRELYSYMAPFVSKSPRAA 484
           +E ETP+ HR G LY+I YV  W+E+  D  K    ++L W+  +Y +M P+VSKSPR A
Sbjct: 424 AEYETPFPHRGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGA 483

Query: 485 YVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIP 538
           YVN++D+D+G      +T Y++ + WG+KYF NNF+RLV+VKT VD  +FF  EQSIP
Sbjct: 484 YVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Lag0025261 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 272/542 (50.18%), Postives = 364/542 (67.16%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MK + +++L F + +  SSS     SS   + F  CL  +S   + I+  ++   N SYS
Sbjct: 1   MKYALILVLFFVVFIWQSSS-----SSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           SVL  +IRNLRF+T  TPKP +II  ++ SH+QAA+ C K H LQ++IRSGGHD++GLSY
Sbjct: 61  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 120

Query: 121 VAY--LPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTV 180
           V Y   PF V+DM NLRSV VD    TAWVQ+GA LGE+YY I EKS+TLA+PAG+CPTV
Sbjct: 121 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 180

Query: 181 GVGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFG 240
           GVGG++SGGGYG M+RKYGL  DN IDA +VD NGK  DR+ MGE L+WAI GGGGGS+G
Sbjct: 181 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 240

Query: 241 IVVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTST 300
           +V+A+K+ LV VP  VT+   +RTLE++A  +IHRWQ VA KL + LF+   +   N + 
Sbjct: 241 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 300

Query: 301 QGGKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGE 360
              KT     F ++FLG    L++IL   FPELGL + DC ETSWI+S L   N  Q G 
Sbjct: 301 SSQKTVRTT-FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-IQVGS 360

Query: 361 VLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGG 420
               LL R+  +  +     K KSDYV+EPI+   +E IW+  K  ++E   +   PYGG
Sbjct: 361 SETLLLQRNQPVNYL-----KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGG 420

Query: 421 RMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRA 480
            M +IS T TP+ +RAG L+KI Y   W+++++    R++   R+LY +M PFVSK+PR 
Sbjct: 421 EMGRISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQ 480

Query: 481 AYVNYRDLDIGSNNKNGE-TSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIP 540
           ++ NYRD+D+G N+ NG+ +SY + + +G KYF  NF+RLVK+KT VD  NFFR+EQSIP
Sbjct: 481 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of Lag0025261 vs. ExPASy TrEMBL
Match: A0A6J1KJP1 (berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.5e-244
Identity = 416/540 (77.04%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MKSS L LL    IVL SSSL  A SS++++DF+ CLLHHSPD++SITKLVYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 VAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VAY+PFIV+D+INL  V VD EK+TAWVQSGA+LGELYYRIAEKSRTLAFPAG+CPTVGV
Sbjct: 121 VAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIV 240
           GG  SGGGYG+++RKYGLAADNV+DAYLVDANG+F DRE MGE LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQG 300
           VAWKVKLVPVPATVT+C+T+++L + AI LI +WQ+VADKLDENLFLGI L G      G
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------G 300

Query: 301 GKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVL 360
           GKTNP+A+FFSLFLGKA++LV IL   FPELGLSKEDCIETSWIE+ALIAANGFQTGEVL
Sbjct: 301 GKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGEVL 360

Query: 361 EALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRM 420
           EALLNRSALIPPI +G  K KSDYVKEP+   AIEG+W R KSQ++E   L  +PYGG+M
Sbjct: 361 EALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGKM 420

Query: 421 SQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAY 480
           S+ISE+E P+ HRAG  Y+I Y++GW EQ V AEKRHLNWIRELYSYM PFVSKSPRA Y
Sbjct: 421 SEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRAVY 480

Query: 481 VNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTLT 540
           VN RDLDIGSN+  G TSY++ARVWGLKYFGNNFDRLV VKTEVD  +FFRHEQSIPTL+
Sbjct: 481 VNCRDLDIGSNDMYGRTSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQSIPTLS 534

BLAST of Lag0025261 vs. ExPASy TrEMBL
Match: A0A6J1EMV2 (berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 5.0e-243
Identity = 414/540 (76.67%), Postives = 468/540 (86.67%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MKSS L LL    IVL SSSL  A SS++++DF+ CLLHHSPD++SITKLVYTPIN+SYS
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+NLRFSTPKTPKPL IITPSNI HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIPHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 VAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VA++PFIV+D+INL SV VD EK+TAWVQSGA+LGELYYRIAEKSRTL FPAG+CPTVGV
Sbjct: 121 VAHVPFIVVDLINLSSVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLTFPAGLCPTVGV 180

Query: 181 GGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIV 240
           GG  SGGGYG+++RKYGLAADNV+DAYLVD NG+F DRE MGE LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDGNGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQG 300
           VAWKVKLVPVPATVT+C+T+++L + AI LI +WQ+VADKLDENLFLGI L G      G
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAISLIDKWQYVADKLDENLFLGIILAG------G 300

Query: 301 GKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVL 360
           GKTNP+A+FFSLFLGKA++LV IL   FPELGL K DCIETSWIE+ALIAANGFQTGEVL
Sbjct: 301 GKTNPLAIFFSLFLGKADELVTILNNAFPELGLPKGDCIETSWIEAALIAANGFQTGEVL 360

Query: 361 EALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRM 420
           EALLNRSALIPPI +G  K KSDYVKEP+   AIEG+W R KSQ++E   L  +PYGG+M
Sbjct: 361 EALLNRSALIPPINSGKSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGKM 420

Query: 421 SQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAY 480
           S+ISE+E P+ HRAG  Y+I Y++GW EQ V AEKRHLNWIRELYSYMAPFVSKSPRAAY
Sbjct: 421 SEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTLT 540
           VN RDLDIGSN+  G TSYQ+ARVWGLKYFGNNFDRL  VKTEVD  +FFRHEQSIPTL+
Sbjct: 481 VNCRDLDIGSNDMYGRTSYQRARVWGLKYFGNNFDRLAHVKTEVDPYDFFRHEQSIPTLS 534

BLAST of Lag0025261 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.4e-237
Identity = 415/542 (76.57%), Postives = 472/542 (87.08%), Query Frame = 0

Query: 3   SSSLILLPFAL--IVLSSSSLCTATSSDKHQDFVGCLLHHSPDS-YSITKLVYTPINSSY 62
           SS LI L F+L  I LSSSSL  A S DKH+ F+ CLL HSPDS YSI+K+VYTP+NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLS 122
           SSVL+FSIRNLRFST  TPKPL IITP ++SH+QAAV+CS++HGLQIR RSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVAYLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVG 182
            V++LPFI+ID+INLRS+ +DA  +TAWVQSGATLGELYY IAEKSRTLAFPAG CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGI 242
           +GG+ SGGGYGLMLRK+GLAADNVIDAYLVDANGKF DRE MGE LFWAIRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQ 302
           VVAWKVKLVPVPATVT C   RTLEE AIKLIHRWQ+VA+KLDENLFLGI L GGN + Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 GG-KTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGE 362
           GG KTNP A FFSLFLGKA++  AIL TTFPELGL+KE+C+E+SWIESAL++A GF+ G+
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGD 363

Query: 363 VLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGG 422
            LEALLNR+    P+T GS KIKSDYVKEPI+EA IEGIW+RLKS+DV A QL  VPYGG
Sbjct: 364 SLEALLNRT----PLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 423

Query: 423 RMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRA 482
           RMSQISE+E P+SHRAG LYKIGY++ WQ+Q+VD EKRHL WIRELYSYM PFVSKSPR+
Sbjct: 424 RMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRS 483

Query: 483 AYVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPT 541
           AYVNYRDLDIGSNNK G+TS+++A VWG KYFGNNF+RLV VKT+VD  +FFRHEQSIPT
Sbjct: 484 AYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 540

BLAST of Lag0025261 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.5e-234
Identity = 402/537 (74.86%), Postives = 459/537 (85.47%), Query Frame = 0

Query: 3   SSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYSSV 62
           SS+LI L    IV+SS+SLC + S  K+Q+F+ CL  HS  SY I+K+VYTPINSSYSSV
Sbjct: 4   SSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSV 63

Query: 63  LNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 122
           L+FSIRNL FS P+TPKPL+IITPS++SHIQAAVICSKSHGLQIR RSGGHDFEGLSYVA
Sbjct: 64  LDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 123

Query: 123 YLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGG 182
           Y PFIV+D+INLRSV V+ E +TAWV+SGATLGELYYRI EKSRTLAFPAGVCPTVG+GG
Sbjct: 124 YRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGG 183

Query: 183 YLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVA 242
           + SGGGYGLMLRK+GLAADNVIDAYLVDANGK  D+ESMGE LFWAIRGGGGGSFGIVVA
Sbjct: 184 HFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVA 243

Query: 243 WKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQGGK 302
           WK+KLVPVP TVTICST R LEEDAIKLIHRWQ+V +KLDEN++LGI LTGGN STQ G 
Sbjct: 244 WKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGI 303

Query: 303 TNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVLEA 362
           TNP A FFSLFLG+A++LV  L T FPELGL K+DC+E SW+ES LI   G Q  E LE 
Sbjct: 304 TNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEP 363

Query: 363 LLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRMSQ 422
           LLNR+      T  S KIKSDY+ EPI  AAI+ IWQRLK+QD+E +QL  VPYGGRMSQ
Sbjct: 364 LLNRTL----TTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 423

Query: 423 ISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAYVN 482
           ISE+ETP+SHRA  L+KIGYV+GW++QS+ A+KRH++W+RE+Y YMAPFVSKSPRAAY N
Sbjct: 424 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 483

Query: 483 YRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTL 540
           YRDLDIGSNNK G+TSY++A VWGLKYFGNNFDRLV VKT+VD  +FFRHEQSIPTL
Sbjct: 484 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Lag0025261 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.5e-234
Identity = 402/537 (74.86%), Postives = 459/537 (85.47%), Query Frame = 0

Query: 3   SSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYSSV 62
           SS+LI L    IV+SS+SLC + S  K+Q+F+ CL  HS  SY I+K+VYTPINSSYSSV
Sbjct: 4   SSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSV 63

Query: 63  LNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 122
           L+FSIRNL FS P+TPKPL+IITPS++SHIQAAVICSKSHGLQIR RSGGHDFEGLSYVA
Sbjct: 64  LDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 123

Query: 123 YLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGG 182
           Y PFIV+D+INLRSV V+ E +TAWV+SGATLGELYYRI EKSRTLAFPAGVCPTVG+GG
Sbjct: 124 YRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGG 183

Query: 183 YLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVA 242
           + SGGGYGLMLRK+GLAADNVIDAYLVDANGK  D+ESMGE LFWAIRGGGGGSFGIVVA
Sbjct: 184 HFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVA 243

Query: 243 WKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQGGK 302
           WK+KLVPVP TVTICST R LEEDAIKLIHRWQ+V +KLDEN++LGI LTGGN STQ G 
Sbjct: 244 WKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGI 303

Query: 303 TNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVLEA 362
           TNP A FFSLFLG+A++LV  L T FPELGL K+DC+E SW+ES LI   G Q  E LE 
Sbjct: 304 TNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEP 363

Query: 363 LLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRMSQ 422
           LLNR+      T  S KIKSDY+ EPI  AAI+ IWQRLK+QD+E +QL  VPYGGRMSQ
Sbjct: 364 LLNRTL----TTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 423

Query: 423 ISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAYVN 482
           ISE+ETP+SHRA  L+KIGYV+GW++QS+ A+KRH++W+RE+Y YMAPFVSKSPRAAY N
Sbjct: 424 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 483

Query: 483 YRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTL 540
           YRDLDIGSNNK G+TSY++A VWGLKYFGNNFDRLV VKT+VD  +FFRHEQSIPTL
Sbjct: 484 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Lag0025261 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 529.3 bits (1362), Expect = 3.8e-150
Identity = 266/541 (49.17%), Postives = 376/541 (69.50%), Query Frame = 0

Query: 2   KSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSS--Y 61
           ++++L L+   LI +  S      SS   QDFV CL+ +S  S+ IT   ++P  ++  +
Sbjct: 8   RNATLFLVTLLLISVPLS------SSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLF 67

Query: 62  SSVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLS 121
              L  + +NLR+ TP  PKP+ I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS
Sbjct: 68  KEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLS 127

Query: 122 YVA--YLPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPT 181
           +VA    PF+++D+  LR V VD + ++AW  +GAT+GE+YYRI EKS+T  FPAG+C +
Sbjct: 128 FVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSS 187

Query: 182 VGVGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSF 241
           +G+GG+L GG YG M+RK+GL ADNV+DA +VDANG+  DR +MGE +FWAIRGGGGGSF
Sbjct: 188 LGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSF 247

Query: 242 GIVVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTS 301
           G+++AWK+KLVPVPATVT+ + T+TLE+D  K++++W+ +ADKLD++LF+ + ++  + +
Sbjct: 248 GVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKT 307

Query: 302 TQGGKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTG 361
           T+ G       + + FLG +N L+ +++ +FPELGL+K+DC E SWI+S +  A GF   
Sbjct: 308 TKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNS 367

Query: 362 EVLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYG 421
              EALL   +L         K KSD+VKEPI    +EG+W+R   +D   T     PYG
Sbjct: 368 AAPEALLAGKSLF----KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYG 427

Query: 422 GRMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPR 481
           G MS+ISE+E P+ HR G L+KI ++  WQ+  V +E+RH+ WIRE+YSYM  +VSK+PR
Sbjct: 428 GMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPR 487

Query: 482 AAYVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIP 539
            AYVNYRDLD+G+N   GET    AR WG KY+  NF+RLVK+K E D +NFFRHEQS+P
Sbjct: 488 QAYVNYRDLDLGTN--EGET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 529

BLAST of Lag0025261 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.2e-148
Identity = 265/538 (49.26%), Postives = 372/538 (69.14%), Query Frame = 0

Query: 5   SLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYSSVLN 64
           S  L    L  L+ S L +A  S+ H+DF+ CL +   D+    K+++T  +SS+ S+L+
Sbjct: 4   SSFLFTILLFSLNISPLVSAHGSN-HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILD 63

Query: 65  FSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAY- 124
            SI+N RFS  +TPKP+ IITP   S +Q  + C++ HG+ +R RS GH +EGLSY+AY 
Sbjct: 64  SSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYN 123

Query: 125 LPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGY 184
            PF VID+ NLRS+ +D +  T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGG 
Sbjct: 124 KPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQ 183

Query: 185 LSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVAW 244
            SGGGYG +LRKYGLAADN+IDA +VDA+G+  DR++MGE  FWAIRGGGG SFG++++W
Sbjct: 184 FSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSW 243

Query: 245 KVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTSTQGGKT 304
           KVKLV VP+T+T+    +T +++A+++I +WQ+ ADK+ ++LF+  TL   N      K 
Sbjct: 244 KVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN------KN 303

Query: 305 NPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVLEAL 364
              ALF  L++G  N+L+A+++  FPELGL KE C E SWIES L  A+ F  GE L  L
Sbjct: 304 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVL 363

Query: 365 LNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGRMSQI 424
            NR       T+ S K K D+V+EPI EAAI+ IW+RL++ +    ++ L P+GG+MS++
Sbjct: 364 TNRER-----TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEM 423

Query: 425 SETETPYSHRAGILYKIGYVIGWQEQSVDAEK----RHLNWIRELYSYMAPFVSKSPRAA 484
           +E ETP+ HR G LY+I YV  W+E+  D  K    ++L W+  +Y +M P+VSKSPR A
Sbjct: 424 AEYETPFPHRGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGA 483

Query: 485 YVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIP 538
           YVN++D+D+G      +T Y++ + WG+KYF NNF+RLV+VKT VD  +FF  EQSIP
Sbjct: 484 YVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Lag0025261 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.9e-148
Identity = 272/542 (50.18%), Postives = 364/542 (67.16%), Query Frame = 0

Query: 1   MKSSSLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPINSSYS 60
           MK + +++L F + +  SSS     SS   + F  CL  +S   + I+  ++   N SYS
Sbjct: 1   MKYALILVLFFVVFIWQSSS-----SSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYS 60

Query: 61  SVLNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           SVL  +IRNLRF+T  TPKP +II  ++ SH+QAA+ C K H LQ++IRSGGHD++GLSY
Sbjct: 61  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 120

Query: 121 VAY--LPFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTV 180
           V Y   PF V+DM NLRSV VD    TAWVQ+GA LGE+YY I EKS+TLA+PAG+CPTV
Sbjct: 121 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 180

Query: 181 GVGGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFG 240
           GVGG++SGGGYG M+RKYGL  DN IDA +VD NGK  DR+ MGE L+WAI GGGGGS+G
Sbjct: 181 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 240

Query: 241 IVVAWKVKLVPVPATVTICSTTRTLEEDAIKLIHRWQFVADKLDENLFLGITLTGGNTST 300
           +V+A+K+ LV VP  VT+   +RTLE++A  +IHRWQ VA KL + LF+   +   N + 
Sbjct: 241 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 300

Query: 301 QGGKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGE 360
              KT     F ++FLG    L++IL   FPELGL + DC ETSWI+S L   N  Q G 
Sbjct: 301 SSQKTVRTT-FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-IQVGS 360

Query: 361 VLEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGG 420
               LL R+  +  +     K KSDYV+EPI+   +E IW+  K  ++E   +   PYGG
Sbjct: 361 SETLLLQRNQPVNYL-----KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGG 420

Query: 421 RMSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRA 480
            M +IS T TP+ +RAG L+KI Y   W+++++    R++   R+LY +M PFVSK+PR 
Sbjct: 421 EMGRISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQ 480

Query: 481 AYVNYRDLDIGSNNKNGE-TSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIP 540
           ++ NYRD+D+G N+ NG+ +SY + + +G KYF  NF+RLVK+KT VD  NFFR+EQSIP
Sbjct: 481 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of Lag0025261 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 259/540 (47.96%), Postives = 370/540 (68.52%), Query Frame = 0

Query: 5   SLILLPFALIVLSSSSLCTATSSDKHQDFVGCLLHHSPDSYSITKLVYTPIN--SSYSSV 64
           S+++L F+L  ++     T++ +     F+ C+  ++   + + K  + P    S +S V
Sbjct: 11  SILVLYFSLYTITP----TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQV 70

Query: 65  LNFSIRNLRFSTPKTPKPLVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 124
           L  + +NLRF     PKP  I +P + SH+QA++ICSK   + +R+RSGGHD+EGLSYV+
Sbjct: 71  LESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVS 130

Query: 125 YL--PFIVIDMINLRSVIVDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 184
            +  PFI++D+  +R V ++ + ++AWVQSGAT+GELYYRIAEKS+   FPAG+C ++G+
Sbjct: 131 QIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGI 190

Query: 185 GGYLSGGGYGLMLRKYGLAADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIV 244
           GG+++GG YG M+RKYGL ADNV+DA +VDANGK  DR +MGE  FWAIRGG GGSFGI+
Sbjct: 191 GGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGII 250

Query: 245 VAWKVKLVPVPATVTICSTTRTLEED-AIKLIHRWQFVADKLDENLFLGITLTGGNTSTQ 304
           +AWK+KLVPVP TVT+ + T+TL++D   K+I +WQ VADKL E LF+ +     N +  
Sbjct: 251 LAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGT 310

Query: 305 GGKTNPVALFFSLFLGKANDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEV 364
           GG       + +LFLG    L+ ++K +FPELGL+ +DCIE SW+ES +   +GF T   
Sbjct: 311 GGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLES-IAYISGFPTHTP 370

Query: 365 LEALLNRSALIPPITTGSGKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFLVPYGGR 424
              LL   +  P +   S K KSD+VK PI E+ ++GI+++L  +D+    +   PYGG 
Sbjct: 371 TNVLLQGKSPFPKV---SFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGM 430

Query: 425 MSQISETETPYSHRAGILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAA 484
           M++I E++ P+ HR G+L+K+ YV  W + S     RH+NWIR+LYSYM P+VS +PR A
Sbjct: 431 MAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREA 490

Query: 485 YVNYRDLDIGSNNKNGETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTL 540
           YVNYRDLD+G N K+ +T  +QA+VWG  YF NNF+RL+ +K +VD ENFFRHEQSIP +
Sbjct: 491 YVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Lag0025261 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 261/526 (49.62%), Postives = 356/526 (67.68%), Query Frame = 0

Query: 23  TATSSDKHQDFVGCLLHHSPDSYSITKLVYTPIN--SSYSSVLNFSIRNLRFSTPKTPKP 82
           T++S+     F+ C+  ++  S+ + K ++TP    S ++ VL  + +NL+F     PKP
Sbjct: 28  TSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKP 87

Query: 83  LVIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYL--PFIVIDMINLRSVI 142
             I  P + S +QA++ICSK  G+  R+RSGGHDFE LSYV+ +  PFI++D+  L+ + 
Sbjct: 88  GFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQIN 147

Query: 143 VDAEKSTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGYLSGGGYGLMLRKYGL 202
           VD E ++AWVQ GATLGELYYRIAEKS+   FPAG+C +VG+GGY++GGGYG ++RKYGL
Sbjct: 148 VDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGL 207

Query: 203 AADNVIDAYLVDANGKFHDRESMGEHLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICS 262
           A DNV+D  +VDANGK  DR +MGE LFWAIRGGGG SFGIV+AWK+KLVPVP TVT+ +
Sbjct: 208 AGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFT 267

Query: 263 TTRTLEEDA-IKLIHRWQFVADKLDENLFLGITLTGGNTSTQGGKTNPVALFFSLFLGKA 322
            T+TLE+DA +K I +WQ ++ K+ E + + + L        G KT  +  +   FLG+ 
Sbjct: 268 VTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRA--AGNDGNKTVTMT-YLGQFLGEK 327

Query: 323 NDLVAILKTTFPELGLSKEDCIETSWIESALIAANGFQTGEVLEALLNRSALIPPITTGS 382
             L+ +++  FPELGL+++DC E SWIE+AL    GF TG  +E LL    L  P+    
Sbjct: 328 GTLLKVMEKAFPELGLTQKDCTEMSWIEAALF-HGGFPTGSPIEILLQ---LKSPLGKDY 387

Query: 383 GKIKSDYVKEPIAEAAIEGIWQRLKSQDVEATQLFL--VPYGGRMSQISETETPYSHRAG 442
            K  SD+VKEPI    ++GI++RL    +E    FL   PYGG MS+I E+  P+ HR G
Sbjct: 388 FKATSDFVKEPIPVIGLKGIFKRL----IEGNTTFLNWTPYGGMMSKIPESAIPFPHRNG 447

Query: 443 ILYKIGYVIGWQEQSVDAEKRHLNWIRELYSYMAPFVSKSPRAAYVNYRDLDIGSNNKNG 502
            L+KI Y   W E       R +NWI+E+Y+YMAP+VS +PR AYVNYRDLD G N  N 
Sbjct: 448 TLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNA 507

Query: 503 ETSYQQARVWGLKYFGNNFDRLVKVKTEVDYENFFRHEQSIPTLTH 542
           + ++ +A++WG KYF  NFDRLVK+KT+VD ENFFRHEQSIP + +
Sbjct: 508 KVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMPY 541