Lag0025166 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0025166
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionphospholipase A1-Igamma1, chloroplastic-like
Locationchr10: 9316338 .. 9317930 (-)
RNA-Seq ExpressionLag0025166
SyntenyLag0025166
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCGAAAATCGTAACACCGAGAGGAGTTCCACTGAAAAAAGTGACGATGTTGAGAAACGGAACGAAGAAAACAAAATGGTATTGGAAATTGAAATTCGGAATCCGACTCAGAGCCATTAAGAACGCTCTTTCATCTTCCTTACACCAGCAGCGCCTCCGTCTGACATGTTCGACCGCCCTGAACGGCCAGCAAACTCCGGTGGCCAACGGCGCCGTTTCGCCACTGGTAATCCAGCCTTCCAAATCGAAGAAGAACGACCTCCGTTTGGCCAAGCCACTGGCTTCCCTCCTCCGAATGCCACTCCGCGCCGCCGACTTCATCGACTACGGCAGCCACATGACTCCCACGCTCTCCCCACGCGAAAACATCTCCGCCATGTGGCGCGACCTCCACGGCGCCGCCGACTGGGCCGGCCTCCTCGACCCTCTACACCCGTTCCTCCGCCGCGAGCTCGTCAAATACGGCGAGTTCGCACAAGCCACCTACGACGCCTTCGACTTCGACCCATTGTCGGAGTTCTGCGGCAGCTGTAGATACAACCGCCACAAGCTCTTCCAAGAACTGGGTCTGGCCCAAAACGGCTACAAAGTCACGAAATACATCTACGCTTTGTCCCCCGTCGACGGCCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTCTGGAGCCGCGACTCGAACTGGATGGGGTTCGTCGCCGTGAGCACCGACGAGGAGAGCGACAGAATCGGCCGTCGCGACATCATGGTAGCGTGGAGAGGAACCGTGACCCCAACGGAATGGTACATCGACCTGAAAACAAAGCTGAAAAAACTCAACCGCAGCGACAAGAAAGTGAAGGTCCAACGTGGGTTCCTAACGATCTACCGATCGAAAGACGAGGAATCAAAATTCAACAAAACGAGCGCGTCGGAGCAAGTGATGGAGGAGCTACACAGACTGGTAGACTTCTTCAAAGAGAAAGGGGACAGAGAGATCAGCGTGACGATCACGGGGCACAGCCTGGGCGGGGCGCTGTCGCTGCTGACGGCGTACGAGGCCGGGGCGTCGTTTCCGGGGGTCCACGTGAGCGTGGTGTCGTTCGGAGCGCCGAGAGTGGGGAACCTGGCGTTCAGGGAGAAGCTGAAGGAGATGGGAGTGAAGACGCTACGAGTGGTGATAAGACAGGACATCGTTCCAAAACTGCCGGGATTGGTGGTGAATTCGATCGTGAACAAGTTGAGCGCAGTGACGGGGAAGTTGAACTGGGTGTACCGACACGTCGGGACGCAGCTGAAGATGGATATGTACATGTCGCCGTACCTGAAGAAGGATAGCGACATGTCGGGGAGCCACAACTTGGAGATTTATATGCATTTGGTGGATGGGTTCGTGAGTAGGAGAGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAATAAAGGGAGTGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAAGGATTGGTCAAGAATAGGTATGGCCGATGGGTCAAGCCTGGCAGGAACCCTGAGGATATTCCTTCTCCATTTTCTCAACCTACCAAGATTTGA

mRNA sequence

ATGGAGGCGAAAATCGTAACACCGAGAGGAGTTCCACTGAAAAAAGTGACGATGTTGAGAAACGGAACGAAGAAAACAAAATGGTATTGGAAATTGAAATTCGGAATCCGACTCAGAGCCATTAAGAACGCTCTTTCATCTTCCTTACACCAGCAGCGCCTCCGTCTGACATGTTCGACCGCCCTGAACGGCCAGCAAACTCCGGTGGCCAACGGCGCCGTTTCGCCACTGGTAATCCAGCCTTCCAAATCGAAGAAGAACGACCTCCGTTTGGCCAAGCCACTGGCTTCCCTCCTCCGAATGCCACTCCGCGCCGCCGACTTCATCGACTACGGCAGCCACATGACTCCCACGCTCTCCCCACGCGAAAACATCTCCGCCATGTGGCGCGACCTCCACGGCGCCGCCGACTGGGCCGGCCTCCTCGACCCTCTACACCCGTTCCTCCGCCGCGAGCTCGTCAAATACGGCGAGTTCGCACAAGCCACCTACGACGCCTTCGACTTCGACCCATTGTCGGAGTTCTGCGGCAGCTGTAGATACAACCGCCACAAGCTCTTCCAAGAACTGGGTCTGGCCCAAAACGGCTACAAAGTCACGAAATACATCTACGCTTTGTCCCCCGTCGACGGCCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTCTGGAGCCGCGACTCGAACTGGATGGGGTTCGTCGCCGTGAGCACCGACGAGGAGAGCGACAGAATCGGCCGTCGCGACATCATGGTAGCGTGGAGAGGAACCGTGACCCCAACGGAATGGTACATCGACCTGAAAACAAAGCTGAAAAAACTCAACCGCAGCGACAAGAAAGTGAAGGTCCAACGTGGGTTCCTAACGATCTACCGATCGAAAGACGAGGAATCAAAATTCAACAAAACGAGCGCGTCGGAGCAAGTGATGGAGGAGCTACACAGACTGGTAGACTTCTTCAAAGAGAAAGGGGACAGAGAGATCAGCGTGACGATCACGGGGCACAGCCTGGGCGGGGCGCTGTCGCTGCTGACGGCGTACGAGGCCGGGGCGTCGTTTCCGGGGGTCCACGTGAGCGTGGTGTCGTTCGGAGCGCCGAGAGTGGGGAACCTGGCGTTCAGGGAGAAGCTGAAGGAGATGGGAGTGAAGACGCTACGAGTGGTGATAAGACAGGACATCGTTCCAAAACTGCCGGGATTGGTGGTGAATTCGATCGTGAACAAGTTGAGCGCAGTGACGGGGAAGTTGAACTGGGTGTACCGACACGTCGGGACGCAGCTGAAGATGGATATGTACATGTCGCCGTACCTGAAGAAGGATAGCGACATGTCGGGGAGCCACAACTTGGAGATTTATATGCATTTGGTGGATGGGTTCGTGAGTAGGAGAGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAATAAAGGGAGTGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAAGGATTGGTCAAGAATAGGTATGGCCGATGGGTCAAGCCTGGCAGGAACCCTGAGGATATTCCTTCTCCATTTTCTCAACCTACCAAGATTTGA

Coding sequence (CDS)

ATGGAGGCGAAAATCGTAACACCGAGAGGAGTTCCACTGAAAAAAGTGACGATGTTGAGAAACGGAACGAAGAAAACAAAATGGTATTGGAAATTGAAATTCGGAATCCGACTCAGAGCCATTAAGAACGCTCTTTCATCTTCCTTACACCAGCAGCGCCTCCGTCTGACATGTTCGACCGCCCTGAACGGCCAGCAAACTCCGGTGGCCAACGGCGCCGTTTCGCCACTGGTAATCCAGCCTTCCAAATCGAAGAAGAACGACCTCCGTTTGGCCAAGCCACTGGCTTCCCTCCTCCGAATGCCACTCCGCGCCGCCGACTTCATCGACTACGGCAGCCACATGACTCCCACGCTCTCCCCACGCGAAAACATCTCCGCCATGTGGCGCGACCTCCACGGCGCCGCCGACTGGGCCGGCCTCCTCGACCCTCTACACCCGTTCCTCCGCCGCGAGCTCGTCAAATACGGCGAGTTCGCACAAGCCACCTACGACGCCTTCGACTTCGACCCATTGTCGGAGTTCTGCGGCAGCTGTAGATACAACCGCCACAAGCTCTTCCAAGAACTGGGTCTGGCCCAAAACGGCTACAAAGTCACGAAATACATCTACGCTTTGTCCCCCGTCGACGGCCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTCTGGAGCCGCGACTCGAACTGGATGGGGTTCGTCGCCGTGAGCACCGACGAGGAGAGCGACAGAATCGGCCGTCGCGACATCATGGTAGCGTGGAGAGGAACCGTGACCCCAACGGAATGGTACATCGACCTGAAAACAAAGCTGAAAAAACTCAACCGCAGCGACAAGAAAGTGAAGGTCCAACGTGGGTTCCTAACGATCTACCGATCGAAAGACGAGGAATCAAAATTCAACAAAACGAGCGCGTCGGAGCAAGTGATGGAGGAGCTACACAGACTGGTAGACTTCTTCAAAGAGAAAGGGGACAGAGAGATCAGCGTGACGATCACGGGGCACAGCCTGGGCGGGGCGCTGTCGCTGCTGACGGCGTACGAGGCCGGGGCGTCGTTTCCGGGGGTCCACGTGAGCGTGGTGTCGTTCGGAGCGCCGAGAGTGGGGAACCTGGCGTTCAGGGAGAAGCTGAAGGAGATGGGAGTGAAGACGCTACGAGTGGTGATAAGACAGGACATCGTTCCAAAACTGCCGGGATTGGTGGTGAATTCGATCGTGAACAAGTTGAGCGCAGTGACGGGGAAGTTGAACTGGGTGTACCGACACGTCGGGACGCAGCTGAAGATGGATATGTACATGTCGCCGTACCTGAAGAAGGATAGCGACATGTCGGGGAGCCACAACTTGGAGATTTATATGCATTTGGTGGATGGGTTCGTGAGTAGGAGAGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAATAAAGGGAGTGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAAGGATTGGTCAAGAATAGGTATGGCCGATGGGTCAAGCCTGGCAGGAACCCTGAGGATATTCCTTCTCCATTTTCTCAACCTACCAAGATTTGA

Protein sequence

MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCSTALNGQQTPVANGAVSPLVIQPSKSKKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPTLSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEESKFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI
Homology
BLAST of Lag0025166 vs. NCBI nr
Match: XP_022927629.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 977.2 bits (2525), Expect = 5.5e-281
Identity = 477/532 (89.66%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNALSSSLH QR RLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSK--SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGAVSPLVIQPSK  SKKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHG ++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF GS
Sbjct: 121 FSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSN 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSD+K+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+DFF +KGDREIS+TI GHSLGGALSLLTAYEAGASFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLKK+SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPFS+P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Lag0025166 vs. NCBI nr
Match: KAG7019492.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 476/532 (89.47%), Postives = 505/532 (94.92%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNALSSSLH QR RLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSK--SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGA SPLVIQPSK  SKKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHG ++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF GS
Sbjct: 121 FSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSN 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSD+K+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+DFF +KGDREIS+TI GHSLGGALSLLTAYEAGASFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLKK+SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPFS+P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Lag0025166 vs. NCBI nr
Match: KAG6583869.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 974.5 bits (2518), Expect = 3.5e-280
Identity = 475/532 (89.29%), Postives = 505/532 (94.92%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNAL+SSLH QR RLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALASSLHNQRRRLTCAT 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSK--SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGA SPLVIQPSK  SKKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHG ++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF GS
Sbjct: 121 FSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSN 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSD+K+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+DFF +KGDREIS+TI GHSLGGALSLLTAYEAGASFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLKK+SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPFS+P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Lag0025166 vs. NCBI nr
Match: XP_023519232.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 474/532 (89.10%), Postives = 505/532 (94.92%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNALSSSLH QR RLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSK--SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGA SPLVIQPSK  SKKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHG ++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF GS
Sbjct: 121 FSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSN 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSD+K+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+DFF +KGDREIS+TI GHSLGGALSLLTAYEAGASFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLK++SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPFS+P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Lag0025166 vs. NCBI nr
Match: XP_023001410.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 970.7 bits (2508), Expect = 5.1e-279
Identity = 474/532 (89.10%), Postives = 503/532 (94.55%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNALSSSLH QR RLTCST
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCST 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSKS--KKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGA SPLVIQPSKS  KKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHGA++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSE+ GS
Sbjct: 121 FSPRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEYRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSD 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSDKK+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+ FF +KGDREIS+TI GHSLGGALSLLTAYEAG SFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVSFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLK++SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPF +P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Lag0025166 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 394.0 bits (1011), Expect = 2.6e-108
Identity = 200/410 (48.78%), Postives = 277/410 (67.56%), Query Frame = 0

Query: 129 WRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQ 188
           WR + G  DWAGL+DP+ P LR EL++YGE AQA YDAFDFDP S++CG+ R+ R + F 
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 189 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 248
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 249 RDIMVAWRGTVTPTEWYIDLKTKLK-----KLNRSDKKVKVQRGFLTIYRSKDEESKFNK 308
           RDI +AWRGTVT  EW  DLK  LK     K+   D  VKV+ GFL +Y  KD   KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 309 TSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYE-----AGASFPG-- 368
            SA EQ++ E+ RLV+   +  D ++S+T+TGHSLGGAL++L+AY+        S  G  
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 369 VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVN-SIVNKLSAVTG 428
           + V+V+++G PRVGN+ FRE+++E+GVK +RVV   D+VPK PGL +N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 429 KLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDV 488
            L W Y HVG +L +D   SP+LK   D+S +HNLE  +HL+DG+  +  +F  +S RD 
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRDH 464

Query: 489 ALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGR-NPEDIPSP 524
           ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 465 ALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Lag0025166 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 3.7e-107
Identity = 189/402 (47.01%), Postives = 265/402 (65.92%), Query Frame = 0

Query: 129 WRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQ 188
           WR + G  DWAGL+DP+ P LR EL++YGE AQA YDAFDFDP S +CGSCR+ R  LF 
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 189 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESD--RIG 248
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 249 RRDIMVAWRGTVTPTEWYIDLKTKLKKLNRS-----DKKVKVQRGFLTIYRSKDEESKFN 308
           RRDI +AWRGTVT  EW  DLK  LK ++ +     D  VK + GFL +Y  KD    F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 309 KTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPG------ 368
           K SA EQV+ E+ RLV+ + ++   E+S+T+TGHSLGGAL++L+AY+             
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 369 -VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNS-IVNKLSAVT 428
            + V+  ++G PRVGN+ F+E+++++GVK LRVV   D+V K PGL +N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 429 GKLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRD 488
           G L W Y HVG  L +D   SP+LK   D+S +HNLE  +HL+DG+  +  +F  +S RD
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRD 448

Query: 489 VALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGR 516
            ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R
Sbjct: 449 PALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of Lag0025166 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 7.1e-98
Identity = 186/427 (43.56%), Postives = 266/427 (62.30%), Query Frame = 0

Query: 124 NISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNR 183
           ++  +WR++ G  +W G LDP++  LRRE+++YGEFAQA YD+FDFDP S++CGSC+Y+ 
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 184 HKLFQELGL-AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES 243
              F  L L    GY +T+Y+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+TDEE 
Sbjct: 146 SDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEE 205

Query: 244 -DRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLN-RSDKKVKVQRGFLTIYRSKDEESKF 303
             R+GRRDI++AWRGTVT  EW  DLK  L   N   D  +K++ GF  +Y  K++  KF
Sbjct: 206 VSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKF 265

Query: 304 NKTSASEQVMEELHRLVDFF-KEKGDREISVTITGHSLGGALSLLTAYEAG--------A 363
           +  SA EQV+ E+ RL++++  E+   + S+T+TGHSLG +L+L++AY+           
Sbjct: 266 SSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPE 325

Query: 364 SFPGVHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSA 423
           +   + ++V SF  PRVGNL F+E+  E+GVK LRVV   D VP +PG+  N        
Sbjct: 326 NNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKY 385

Query: 424 VTGKLN--WVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSR----RGK 483
           V  K +  W Y HVG +L +D   SP+LK   D+  +HNLE  +HLVDG+  +      +
Sbjct: 386 VEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKR 445

Query: 484 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGR------NPED 527
           F   ++RD+ALVNK  D L  E  +P  W Q  +KG+VKN  G+WV P R       PED
Sbjct: 446 FCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPED 505

BLAST of Lag0025166 vs. ExPASy Swiss-Prot
Match: B9EYD3 (Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 PE=2 SV=2)

HSP 1 Score: 266.5 bits (680), Expect = 6.2e-70
Identity = 162/408 (39.71%), Postives = 222/408 (54.41%), Query Frame = 0

Query: 118 TLSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCG 177
           T +PR  ++  WR+LHG   W GLLDPL   LRR ++ YGE AQAT DAF  +  S   G
Sbjct: 3   TTAPRA-VAERWRELHGEDHWKGLLDPLDADLRRSVIGYGELAQATNDAFIREAWSPHAG 62

Query: 178 SCRYNRHKLFQELGLA---QNGYKVTKYIYALSPVDG-PDWFESSKIGEVWSRDSNWMGF 237
           +CRY+R +  ++   +      Y+VT + YA +   G P  F       V +R+SNWMG+
Sbjct: 63  ACRYSRDRFLEKAQASTQLAGLYEVTAFFYATAGAGGVPAPF------MVRNRESNWMGY 122

Query: 238 VAVSTDEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKK------LNRSDKKVKVQRGF 297
           VAV+TD     +GRRD++VAWRGTV P EW  DL   L                +V RG+
Sbjct: 123 VAVATDAGVAALGRRDVVVAWRGTVRPMEWLNDLDFTLVSAAGVLGAGGRSPAPRVHRGW 182

Query: 298 LTIYRSKDEESKFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAY 357
           L+IY + D  SK++K SA EQ+ +E+ RL+D +K   D E S+T+ GHSLG A++ L A 
Sbjct: 183 LSIYTASDPASKYSKLSAREQISDEIKRLMDKYK---DEETSITVVGHSLGAAVATLNAA 242

Query: 358 EAGASFPGVH----VSVVSFGAPRVGNLAFREKLKEM-GVKTLRVVIRQDIVPKLPGLVV 417
           +  ++    H    V+ V+F  PRVG+  FR+   E+ G++ LRV    D+VPK P +  
Sbjct: 243 DIVSNGLNQHGACPVTAVAFACPRVGDSGFRKLFDELPGLRLLRVCNSPDVVPKYPPM-- 302

Query: 418 NSIVNKLSAVTGKLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSR 477
                            Y  VG +L +D   SPYLK   + +  H+LE YMH V G   +
Sbjct: 303 ----------------GYADVGVELPVDTRRSPYLKSPGNQAVWHSLECYMHGVAGAQGK 362

Query: 478 RGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRW 511
           RG F+    RDVALVNK  D L EE  +P  W     KG+V+   G W
Sbjct: 363 RGGFKLEVDRDVALVNKNVDALKEEYHVPPSWSVQRDKGMVRGADGHW 382

BLAST of Lag0025166 vs. ExPASy Swiss-Prot
Match: A2WT95 (Phospholipase A1-II 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_03083 PE=3 SV=2)

HSP 1 Score: 263.8 bits (673), Expect = 4.1e-69
Identity = 155/397 (39.04%), Postives = 214/397 (53.90%), Query Frame = 0

Query: 124 NISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNR 183
           NI+  WR+L+G + W GLLDPL   LR  ++ YGE +QA Y   + +  S + GSC ++R
Sbjct: 9   NIARRWRELNGVSYWKGLLDPLDVDLRNNIINYGELSQAAYTGLNRERRSRYAGSCLFSR 68

Query: 184 HKLFQELGLAQ-NGYKVTKYIYALSPVDGPDWFE-SSKIGEVWSRDSNWMGFVAVSTDEE 243
                 + ++  N Y +TK+IYA+  V  PD F   S     WS+ SNWMGFVAV+TDE 
Sbjct: 69  KDFLSRVDVSNPNLYVITKFIYAMCTVSLPDAFMIKSWSKAAWSKQSNWMGFVAVATDEG 128

Query: 244 SDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNR-----SDKKVKVQRGFLTIYRSKDE 303
            + +GRRD++VAWRGT+   EW  DL   L   +      S     V  G+L++Y S D 
Sbjct: 129 KEVLGRRDVVVAWRGTIRMVEWMDDLDISLVPASEIVRPGSADDPCVHGGWLSVYTSADP 188

Query: 304 ESKFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYE--AGASFP 363
           ES++NK SA  QV+ E+ RL D ++ +   E S+TITGHSLG AL+ + A +  +     
Sbjct: 189 ESQYNKQSARYQVLNEIKRLQDMYEHE---ETSITITGHSLGAALATINATDIVSNGYNK 248

Query: 364 GVHVSVVSFGAPRVGNLAFREKLKEM-GVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVT 423
              VS   FG+PRVGN  F++       ++ LR+    D+VP  P L             
Sbjct: 249 SCPVSAFVFGSPRVGNPDFQKAFDSAPDLRLLRIRNSPDVVPNWPKL------------- 308

Query: 424 GKLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRD 483
                 Y   GT+L +D   SPYLK   +    H++E YMH V G     G F+    RD
Sbjct: 309 -----GYSDAGTELMIDTGKSPYLKAPGNPLTWHDMECYMHGVAGTQGSNGGFKLEIDRD 368

Query: 484 VALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRW 511
           +ALVNK  D L  E  IP  W+ + +KG+VK   GRW
Sbjct: 369 IALVNKHEDALKNEYAIPSSWWVVQNKGMVKGTDGRW 384

BLAST of Lag0025166 vs. ExPASy TrEMBL
Match: A0A6J1EIJ3 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434398 PE=4 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.6e-281
Identity = 477/532 (89.66%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNALSSSLH QR RLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSK--SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGAVSPLVIQPSK  SKKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHG ++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF GS
Sbjct: 121 FSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSN 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSD+K+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+DFF +KGDREIS+TI GHSLGGALSLLTAYEAGASFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLKK+SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPFS+P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Lag0025166 vs. ExPASy TrEMBL
Match: A0A6J1KL46 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495553 PE=4 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.5e-279
Identity = 474/532 (89.10%), Postives = 503/532 (94.55%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           ME +IVTPRGVPLK+VTMLRNGTKKTKWYWKLK+GIRLRAIKNALSSSLH QR RLTCST
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCST 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSKS--KKNDLRLAKPLASLLRMPLRAADFIDYGSHMTPT 120
           A  GQ TPV NGA SPLVIQPSKS  KKNDLRLAKPLASLLRMP RAADF+DYG+HMTPT
Sbjct: 61  A-TGQLTPV-NGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPT 120

Query: 121 LSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGS 180
            SPR+ I+ +WRDLHGA++W GLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSE+ GS
Sbjct: 121 FSPRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEYRGS 180

Query: 181 CRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVST 240
           CRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS 
Sbjct: 181 CRYNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSD 240

Query: 241 DEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEES 300
           DEES+RIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RSDKK+KVQRGFLTIY+SKDEES
Sbjct: 241 DEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYKSKDEES 300

Query: 301 KFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHV 360
           KFNKTSASEQVMEELHRL+ FF +KGDREIS+TI GHSLGGALSLLTAYEAG SFP +HV
Sbjct: 301 KFNKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVSFPDIHV 360

Query: 361 SVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNW 420
           SV+SFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKLSAVTGKLNW
Sbjct: 361 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 420

Query: 421 VYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVN 480
           +YRHVGTQLKMDMYMSPYLK++SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDVALVN
Sbjct: 421 LYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 480

Query: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           KGSDMLVEELRIPEFWYQLPHKGLVKNR+GRWVKPGRN EDIPSPF +P+ I
Sbjct: 481 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Lag0025166 vs. ExPASy TrEMBL
Match: A0A5A7UFJ9 (Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001160 PE=4 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.2e-279
Identity = 471/535 (88.04%), Postives = 504/535 (94.21%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           MEAK+ TPRG+P+K+ TMLRNGTKKTKWYWKLK GIR RAIK ALSSSLH QR RLTCST
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 61  ALNGQQTP---VANGAVSPLVIQPSK-SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMT 120
           A+    TP     NG VSPLVI  SK SKKNDLRLAKPLASLLRMPLRAADFID+G+HMT
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSKPSKKNDLRLAKPLASLLRMPLRAADFIDFGNHMT 120

Query: 121 PTLSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFC 180
           PTLSPR+NISA+WRDLHGA+DW GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEFC
Sbjct: 121 PTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 181 GSCRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240
           GSCRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VAV
Sbjct: 181 GSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVAV 240

Query: 241 STDEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDE 300
           S+DEESDRIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RS+KKVKVQRGFLTIY+SKDE
Sbjct: 241 SSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 301 ESKFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPG- 360
           +SKFNKTSASEQVMEELHRL+DFFKEKGDREIS+TITGHSLGGALSLLTAYEAG +FP  
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 361 VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGK 420
           VHVSVVSFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKL AVTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTGK 420

Query: 421 LNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVA 480
           LNWVYRHVG +L+M+MYMSPYLKKDSDMSGSHNLEIY+HLVDGFV+++GKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDVA 480

Query: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           LVNKGSDMLVEELRIPEFWYQLPHKGL+KNR+GRWVKPGRNPEDIPSPFSQP ++
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Lag0025166 vs. ExPASy TrEMBL
Match: A0A1S3BBX3 (phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 PE=4 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.2e-279
Identity = 471/535 (88.04%), Postives = 504/535 (94.21%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           MEAK+ TPRG+P+K+ TMLRNGTKKTKWYWKLK GIR RAIK ALSSSLH QR RLTCST
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 61  ALNGQQTP---VANGAVSPLVIQPSK-SKKNDLRLAKPLASLLRMPLRAADFIDYGSHMT 120
           A+    TP     NG VSPLVI  SK SKKNDLRLAKPLASLLRMPLRAADFID+G+HMT
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSKPSKKNDLRLAKPLASLLRMPLRAADFIDFGNHMT 120

Query: 121 PTLSPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFC 180
           PTLSPR+NISA+WRDLHGA+DW GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEFC
Sbjct: 121 PTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 181 GSCRYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240
           GSCRYNRHKL  ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VAV
Sbjct: 181 GSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVAV 240

Query: 241 STDEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDE 300
           S+DEESDRIGRRDI+VAWRGTVTPTEWYIDLKTKLKK++RS+KKVKVQRGFLTIY+SKDE
Sbjct: 241 SSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 301 ESKFNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPG- 360
           +SKFNKTSASEQVMEELHRL+DFFKEKGDREIS+TITGHSLGGALSLLTAYEAG +FP  
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 361 VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGK 420
           VHVSVVSFGAPRVGNLAFREKL EMGVKTLRVVIRQDIVPKLPGL VNSIVNKL AVTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTGK 420

Query: 421 LNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVA 480
           LNWVYRHVG +L+M+MYMSPYLKKDSDMSGSHNLEIY+HLVDGFV+++GKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDVA 480

Query: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           LVNKGSDMLVEELRIPEFWYQLPHKGL+KNR+GRWVKPGRNPEDIPSPFSQP ++
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Lag0025166 vs. ExPASy TrEMBL
Match: A0A6J1GSS0 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456800 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 2.0e-273
Identity = 461/531 (86.82%), Postives = 497/531 (93.60%), Query Frame = 0

Query: 1   MEAKIVTPRGVPLKKVTMLRNGTKKTKWYWKLKFGIRLRAIKNALSSSLHQQRLRLTCST 60
           M+AK+VTPR VPLKKVTMLRNGTKKTKWYWKLK GIRLRAIKNA S+S+HQ R  LTCST
Sbjct: 1   MDAKMVTPRTVPLKKVTMLRNGTKKTKWYWKLKLGIRLRAIKNAFSTSVHQHRRYLTCST 60

Query: 61  ALNGQQTPVANGAVSPLVIQPSKSKKNDLRLAKPLASLLRMPLRAADFIDYGS-HMTPTL 120
           A++GQQTP+ NGA+SP+VI+    KKNDLRL KPLASLLRMPLRA DFIDYG+ HMTPT+
Sbjct: 61  AVHGQQTPI-NGAISPIVIKHKNPKKNDLRLTKPLASLLRMPLRAPDFIDYGNHHMTPTI 120

Query: 121 SPRENISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSC 180
           SPRENISA+WRDLHGA +WA LLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSC
Sbjct: 121 SPRENISAVWRDLHGAGNWADLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSC 180

Query: 181 RYNRHKLFQELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTD 240
           RYNRHKL  ELGLAQNGYKVTK+IYALSPVDGPDWFE SKIG+VWSRDSNWMGFVAVSTD
Sbjct: 181 RYNRHKLLHELGLAQNGYKVTKFIYALSPVDGPDWFERSKIGKVWSRDSNWMGFVAVSTD 240

Query: 241 EESDRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLNRSDKKVKVQRGFLTIYRSKDEESK 300
           EESDRIGRRDI+VAWRGTVTPTEWYID+KTKLKK++RS+K VKVQRGFLTIYRSKDE+S+
Sbjct: 241 EESDRIGRRDILVAWRGTVTPTEWYIDMKTKLKKIDRSNKNVKVQRGFLTIYRSKDEQSQ 300

Query: 301 FNKTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPGVHVS 360
           FNKTSASEQVMEELHRL++FFK+K DREIS+TITGHSLGGALSLLTAYEAG SFP +H+S
Sbjct: 301 FNKTSASEQVMEELHRLIEFFKQKNDREISLTITGHSLGGALSLLTAYEAGVSFPDIHIS 360

Query: 361 VVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNWV 420
           V+SFGAPRVGNLAF+EKL EMGVK LRVVIRQDIVPKLPGLVVNSI+NKLSAVT KLNWV
Sbjct: 361 VMSFGAPRVGNLAFKEKLNEMGVKALRVVIRQDIVPKLPGLVVNSIMNKLSAVTKKLNWV 420

Query: 421 YRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVNK 480
           YRHVGT +KMDM MSPYLKK+SDMSGSHNLEIY+HLVDGFVSRRGKFRWNSRRDV LVNK
Sbjct: 421 YRHVGTPMKMDMNMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVTLVNK 480

Query: 481 GSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGRNPEDIPSPFSQPTKI 531
           GSDMLVEELRIPEFWYQLPHKGLVKN YGRWVK GRN EDIPSP  QP+KI
Sbjct: 481 GSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKLGRNHEDIPSPLIQPSKI 530

BLAST of Lag0025166 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.4e-170
Identity = 284/439 (64.69%), Postives = 357/439 (81.32%), Query Frame = 0

Query: 93  KPLASLLRMPLRAA-DFIDYGSHMTPTLSPRENISAMWRDLHGAADWAGLLDPLHPFLRR 152
           K LA LL++P  +A DF+D G  MTP  SPRE IS MWR+LHG+ +W  LLDPLHP+LRR
Sbjct: 81  KSLAFLLQLPKYSADDFLDRGDLMTPAASPREKISKMWRELHGSNNWENLLDPLHPWLRR 140

Query: 153 ELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQELGLAQNGYKVTKYIYALSPVDG 212
           E+ KYGEF ++ YD+ DFDPLSEFCGS RYNR+KLF+ELGL ++GYKVTKYIYA+S VD 
Sbjct: 141 EVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRNKLFEELGLTRHGYKVTKYIYAMSRVDV 200

Query: 213 PDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIGRRDIMVAWRGTVTPTEWYIDLKTKL 272
           P WF SS +GE WS+DSNWMGFVAVS D ES RIGRRDI+VAWRGTVTPTEW++DL+T +
Sbjct: 201 PQWFLSSALGETWSKDSNWMGFVAVSGDRESLRIGRRDIVVAWRGTVTPTEWFMDLRTSM 260

Query: 273 KKLNRSDKK----VKVQRGFLTIYRSKDEESKFNKTSASEQVMEELHRLVDFFKEKGDRE 332
           +  +   K     VKVQ GFL+IY SK E +++NK SASEQ M+E+ RLV+FFK++G+ E
Sbjct: 261 EPFDCEGKHGKTVVKVQSGFLSIYNSKSELTRYNKESASEQTMDEVKRLVNFFKDRGE-E 320

Query: 333 ISVTITGHSLGGALSLLTAYEAGASFPGV--HVSVVSFGAPRVGNLAFREKLKEMGVKTL 392
           +S+TITGHSLGGAL+L+ AYEA    P +  ++SV+SFGAPRVGNLAF+EKL  +GVK L
Sbjct: 321 VSLTITGHSLGGALALMNAYEAARDVPALSGNISVISFGAPRVGNLAFKEKLNSLGVKVL 380

Query: 393 RVVIRQDIVPKLPGLVVNSIVNKLSAVTGKLNWVYRHVGTQLKMDMYMSPYLKKDSDMSG 452
           RVV +QDIVPKLPG+V N ++NKL+ +T +LNWVYRHVGTQLK+D++ SPY+K+DSD+  
Sbjct: 381 RVVNKQDIVPKLPGIVFNKVLNKLNPITSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGR 440

Query: 453 SHNLEIYMHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKN 512
           +HNLE+Y+H++DGF  ++  FR N+RRDVA VNK +DML++ LRIPEFWYQ+ HKGL+ N
Sbjct: 441 AHNLEVYLHVLDGFHRKKSGFRVNARRDVASVNKSTDMLLDHLRIPEFWYQVAHKGLILN 500

Query: 513 RY-GRWVKPGRNPEDIPSP 524
           +  GRWVKP R PEDIPSP
Sbjct: 501 KQTGRWVKPVRAPEDIPSP 518

BLAST of Lag0025166 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-109
Identity = 200/410 (48.78%), Postives = 277/410 (67.56%), Query Frame = 0

Query: 129 WRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQ 188
           WR + G  DWAGL+DP+ P LR EL++YGE AQA YDAFDFDP S++CG+ R+ R + F 
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 189 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 248
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 249 RDIMVAWRGTVTPTEWYIDLKTKLK-----KLNRSDKKVKVQRGFLTIYRSKDEESKFNK 308
           RDI +AWRGTVT  EW  DLK  LK     K+   D  VKV+ GFL +Y  KD   KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 309 TSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYE-----AGASFPG-- 368
            SA EQ++ E+ RLV+   +  D ++S+T+TGHSLGGAL++L+AY+        S  G  
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 369 VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVN-SIVNKLSAVTG 428
           + V+V+++G PRVGN+ FRE+++E+GVK +RVV   D+VPK PGL +N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 429 KLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRDV 488
            L W Y HVG +L +D   SP+LK   D+S +HNLE  +HL+DG+  +  +F  +S RD 
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRDH 464

Query: 489 ALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGR-NPEDIPSP 524
           ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 465 ALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Lag0025166 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.7e-108
Identity = 189/402 (47.01%), Postives = 265/402 (65.92%), Query Frame = 0

Query: 129 WRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQ 188
           WR + G  DWAGL+DP+ P LR EL++YGE AQA YDAFDFDP S +CGSCR+ R  LF 
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 189 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESD--RIG 248
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 249 RRDIMVAWRGTVTPTEWYIDLKTKLKKLNRS-----DKKVKVQRGFLTIYRSKDEESKFN 308
           RRDI +AWRGTVT  EW  DLK  LK ++ +     D  VK + GFL +Y  KD    F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 309 KTSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYEAGASFPG------ 368
           K SA EQV+ E+ RLV+ + ++   E+S+T+TGHSLGGAL++L+AY+             
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 369 -VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNS-IVNKLSAVT 428
            + V+  ++G PRVGN+ F+E+++++GVK LRVV   D+V K PGL +N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 429 GKLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSRRGKFRWNSRRD 488
           G L W Y HVG  L +D   SP+LK   D+S +HNLE  +HL+DG+  +  +F  +S RD
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRD 448

Query: 489 VALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGR 516
            ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R
Sbjct: 449 PALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of Lag0025166 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 5.0e-99
Identity = 186/427 (43.56%), Postives = 266/427 (62.30%), Query Frame = 0

Query: 124 NISAMWRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNR 183
           ++  +WR++ G  +W G LDP++  LRRE+++YGEFAQA YD+FDFDP S++CGSC+Y+ 
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 184 HKLFQELGL-AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES 243
              F  L L    GY +T+Y+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+TDEE 
Sbjct: 146 SDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEE 205

Query: 244 -DRIGRRDIMVAWRGTVTPTEWYIDLKTKLKKLN-RSDKKVKVQRGFLTIYRSKDEESKF 303
             R+GRRDI++AWRGTVT  EW  DLK  L   N   D  +K++ GF  +Y  K++  KF
Sbjct: 206 VSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKF 265

Query: 304 NKTSASEQVMEELHRLVDFF-KEKGDREISVTITGHSLGGALSLLTAYEAG--------A 363
           +  SA EQV+ E+ RL++++  E+   + S+T+TGHSLG +L+L++AY+           
Sbjct: 266 SSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPE 325

Query: 364 SFPGVHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVNSIVNKLSA 423
           +   + ++V SF  PRVGNL F+E+  E+GVK LRVV   D VP +PG+  N        
Sbjct: 326 NNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKY 385

Query: 424 VTGKLN--WVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVSR----RGK 483
           V  K +  W Y HVG +L +D   SP+LK   D+  +HNLE  +HLVDG+  +      +
Sbjct: 386 VEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKR 445

Query: 484 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRYGRWVKPGR------NPED 527
           F   ++RD+ALVNK  D L  E  +P  W Q  +KG+VKN  G+WV P R       PED
Sbjct: 446 FCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPED 505

BLAST of Lag0025166 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 1.3e-94
Identity = 171/347 (49.28%), Postives = 239/347 (68.88%), Query Frame = 0

Query: 129 WRDLHGAADWAGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFQ 188
           WR + G  DWAGL+DP+ P LR EL++YGE AQA YDAFDFDP S++CG+ R+ R + F 
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 189 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 248
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 249 RDIMVAWRGTVTPTEWYIDLKTKLK-----KLNRSDKKVKVQRGFLTIYRSKDEESKFNK 308
           RDI +AWRGTVT  EW  DLK  LK     K+   D  VKV+ GFL +Y  KD   KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 309 TSASEQVMEELHRLVDFFKEKGDREISVTITGHSLGGALSLLTAYE-----AGASFPG-- 368
            SA EQ++ E+ RLV+   +  D ++S+T+TGHSLGGAL++L+AY+        S  G  
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 369 VHVSVVSFGAPRVGNLAFREKLKEMGVKTLRVVIRQDIVPKLPGLVVN-SIVNKLSAVTG 428
           + V+V+++G PRVGN+ FRE+++E+GVK +RVV   D+VPK PGL +N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 429 KLNWVYRHVGTQLKMDMYMSPYLKKDSDMSGSHNLEIYMHLVDGFVS 462
            L W Y HVG +L +D   SP+LK   D+S +HNLE  +HL+DG+VS
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022927629.15.5e-28189.66phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata][more]
KAG7019492.11.6e-28089.47Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
KAG6583869.13.5e-28089.29Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023519232.11.3e-27989.10phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023001410.15.1e-27989.10phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q3EBR62.6e-10848.78Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Q941F13.7e-10747.01Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4... [more]
Q9C8J67.1e-9843.56Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2... [more]
B9EYD36.2e-7039.71Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 P... [more]
A2WT954.1e-6939.04Phospholipase A1-II 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_03083 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A6J1EIJ32.6e-28189.66phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KL462.5e-27989.10phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5A7UFJ93.2e-27988.04Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BBX33.2e-27988.04phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 ... [more]
A0A6J1GSS02.0e-27386.82phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G30370.12.4e-17064.69alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.21.8e-10948.78alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.12.7e-10847.01alpha/beta-Hydrolases superfamily protein [more]
AT1G51440.15.0e-9943.56alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.11.3e-9449.28alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 252..400
e-value: 2.8E-34
score: 118.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 128..522
e-value: 6.0E-129
score: 432.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 194..457
NoneNo IPR availablePANTHERPTHR31403:SF13ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 12..528
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 12..528
NoneNo IPR availableCDDcd00519Lipase_3coord: 182..457
e-value: 4.56788E-48
score: 164.572

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0025166.1Lag0025166.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004620 phospholipase activity