Lag0025149 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0025149
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionEndochitinase
Locationchr10: 9086790 .. 9088679 (+)
RNA-Seq ExpressionLag0025149
SyntenyLag0025149
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTACTCCGATTCCGCAAATTAGGGCGAAATTTCTCCCCACACTTCTTCTCCTGATCGGACTCCTCGCCGGAACCGCCGTGGTGGCTCAGAATTGCGGCTGCAAAGCCGGCTCTATTTGCTGCAGCCGATCTAGCTTCTGCGGCTCCGGCAACAAATTATGCGGATTCGGCTGCAGAGAGTTTCTGAATGCGTGGAAGCCTCTCATCTCTGATACCGAGAATGAGATTGCTGGCGCTCCTAAGTGCGGCTGCTCCGCCGGACTCTGCTGCAGCCGGCACGGCTTCTGTGGCGTTGGCGACGGCTACTGCGGCGCCGGATGTCGAGAAGGACCGTGTAAAGCGGCTCCGGCGCCGAGTCCCGGCATGAACGGCACGGTGGTGGCCGACATCGTGACGGAAGGTTTGTTCGAGAACATTACTGGTGGAGGCGATGAGCGGTGTGCCGGAAAAAGGTTTTATAGCAGAAAGGCGTTTGTGGAAGCTGCGGAATCGTTCGATGAATTTGGTAGAATTGGTTCTGTGGATGATTCAAAGAGGGAAATTGCTGCGTTTTTCAGTCACGTCACTTATGAGTCTGGAAGTAAGATAACGACTCTCTCCTTCCTACAGATTCATTTCAAATCAATTAAAGAAATAATGCTTAAATTATTTTTTTAAAAAAAACACCACTTGAATTACCTTTTTAGTTCATTGGGCTTCAGGTTTATATATTCGAAGTATTGTACATGTTAGATTATATATTTTTTTTTTCTTTTTTTTAGAATAGATTATAACTTAGTTCTTAAACCATTTTTGTGTTTATTACATCCTGAACTTAAAAAAAAAAAAAAAAAAAAAAAAATCCAAGTCTCTACAAATTTTGCTTTCTTGAACTTAAAAATGTTAGATAGGTTGCAACAATTTTTTTTTTTAATTTTTAAAATTAAGTTTATAAATACTATTTTTTTTTCTTGAAAATCTTATAAATACTATTTTCACAGTTTATTTGTTTTTGTTAATGACTGTTTACAAAAATCAAGTCAAGTTTTAAAAATTAAAAAAACTAATTTTCAAAAACTTGCTTTTGTTTTTTAAATTTAGGTAGAAATTTCATCAAAGATGAAAAACATAGTAAGGAAATTTGAAAAAAAAAAGTCATCAAATGGGTCTAAACTTTGTTCACAACATATACAATATCTTTTCAAAATTAATGAATCTATTAGACATTAAATTGAAATTGGGGTAATTATGAAGCTCTATTTGATAGTCATTTTGTTTTTAATACACTACTTTCTATCTATAAGTTTTCTACTAATTCGTTATCTACTTTCATCAATGTTTTCAAAACCCAATTAAAAAAAAGTAGTTTCTGTTTCAATACTTGATTAATAATTCAAATGTTACATTAACGATGAGGAAAATCATGGTAAAAAAAAAATAGAGAAAAACAAAGAAACATAATTATGAAAATTCCAAGTGTATTTTGTTTGTTTGTGCATAGACTTGTGCTTCGTAGAAGAAATAGACGGGGATGCAGGTGACTACTGCGACAAGGAAAAAACCCAGTACCCGTGCAACCCAAGCAAGAGGTACTTCGGGCGAGGCCCAATCCAACTATCGTGGAACTTCAACTACGGCCCAGCAGGCGAGGCCATCGGGTTCGACGGCCTCAACAACCCTGAAATCGTCGCCTCCGACCCGGAGGTGTCCTTCGAGACGGCGCTCTGGTACTGGATGAACAATGTTCACTCTGTCATCGACCAAGGTTTTGGGGCAACCATTCGAGCCGTTAATGGTGCTGTTGAATGCGACGGTGGTAACCCGGGTGCGGTGCAACGACGAGTGATGCTCTACACTCAATACTGTGACCAACTCGGTGTTGCTCCGGGCCATAATCTTACATGTTGA

mRNA sequence

ATGTCTACTCCGATTCCGCAAATTAGGGCGAAATTTCTCCCCACACTTCTTCTCCTGATCGGACTCCTCGCCGGAACCGCCGTGGTGGCTCAGAATTGCGGCTGCAAAGCCGGCTCTATTTGCTGCAGCCGATCTAGCTTCTGCGGCTCCGGCAACAAATTATGCGGATTCGGCTGCAGAGAGTTTCTGAATGCGTGGAAGCCTCTCATCTCTGATACCGAGAATGAGATTGCTGGCGCTCCTAAGTGCGGCTGCTCCGCCGGACTCTGCTGCAGCCGGCACGGCTTCTGTGGCGTTGGCGACGGCTACTGCGGCGCCGGATGTCGAGAAGGACCGTGTAAAGCGGCTCCGGCGCCGAGTCCCGGCATGAACGGCACGGTGGTGGCCGACATCGTGACGGAAGGTTTGTTCGAGAACATTACTGGTGGAGGCGATGAGCGGTGTGCCGGAAAAAGGTTTTATAGCAGAAAGGCGTTTGTGGAAGCTGCGGAATCGTTCGATGAATTTGGTAGAATTGGTTCTGTGGATGATTCAAAGAGGGAAATTGCTGCGTTTTTCAGTCACGTCACTTATGAGTCTGGAAACTTGTGCTTCGTAGAAGAAATAGACGGGGATGCAGGTGACTACTGCGACAAGGAAAAAACCCAGTACCCGTGCAACCCAAGCAAGAGGTACTTCGGGCGAGGCCCAATCCAACTATCGTGGAACTTCAACTACGGCCCAGCAGGCGAGGCCATCGGGTTCGACGGCCTCAACAACCCTGAAATCGTCGCCTCCGACCCGGAGGTGTCCTTCGAGACGGCGCTCTGGTACTGGATGAACAATGTTCACTCTGTCATCGACCAAGGTTTTGGGGCAACCATTCGAGCCGTTAATGGTGCTGTTGAATGCGACGGTGGTAACCCGGGTGCGGTGCAACGACGAGTGATGCTCTACACTCAATACTGTGACCAACTCGGTGTTGCTCCGGGCCATAATCTTACATGTTGA

Coding sequence (CDS)

ATGTCTACTCCGATTCCGCAAATTAGGGCGAAATTTCTCCCCACACTTCTTCTCCTGATCGGACTCCTCGCCGGAACCGCCGTGGTGGCTCAGAATTGCGGCTGCAAAGCCGGCTCTATTTGCTGCAGCCGATCTAGCTTCTGCGGCTCCGGCAACAAATTATGCGGATTCGGCTGCAGAGAGTTTCTGAATGCGTGGAAGCCTCTCATCTCTGATACCGAGAATGAGATTGCTGGCGCTCCTAAGTGCGGCTGCTCCGCCGGACTCTGCTGCAGCCGGCACGGCTTCTGTGGCGTTGGCGACGGCTACTGCGGCGCCGGATGTCGAGAAGGACCGTGTAAAGCGGCTCCGGCGCCGAGTCCCGGCATGAACGGCACGGTGGTGGCCGACATCGTGACGGAAGGTTTGTTCGAGAACATTACTGGTGGAGGCGATGAGCGGTGTGCCGGAAAAAGGTTTTATAGCAGAAAGGCGTTTGTGGAAGCTGCGGAATCGTTCGATGAATTTGGTAGAATTGGTTCTGTGGATGATTCAAAGAGGGAAATTGCTGCGTTTTTCAGTCACGTCACTTATGAGTCTGGAAACTTGTGCTTCGTAGAAGAAATAGACGGGGATGCAGGTGACTACTGCGACAAGGAAAAAACCCAGTACCCGTGCAACCCAAGCAAGAGGTACTTCGGGCGAGGCCCAATCCAACTATCGTGGAACTTCAACTACGGCCCAGCAGGCGAGGCCATCGGGTTCGACGGCCTCAACAACCCTGAAATCGTCGCCTCCGACCCGGAGGTGTCCTTCGAGACGGCGCTCTGGTACTGGATGAACAATGTTCACTCTGTCATCGACCAAGGTTTTGGGGCAACCATTCGAGCCGTTAATGGTGCTGTTGAATGCGACGGTGGTAACCCGGGTGCGGTGCAACGACGAGTGATGCTCTACACTCAATACTGTGACCAACTCGGTGTTGCTCCGGGCCATAATCTTACATGTTGA

Protein sequence

MSTPIPQIRAKFLPTLLLLIGLLAGTAVVAQNCGCKAGSICCSRSSFCGSGNKLCGFGCREFLNAWKPLISDTENEIAGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCDQLGVAPGHNLTC
Homology
BLAST of Lag0025149 vs. NCBI nr
Match: XP_022140102.1 (endochitinase EP3-like [Momordica charantia])

HSP 1 Score: 401.7 bits (1031), Expect = 5.9e-108
Identity = 182/252 (72.22%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG+GCREGPC   P  SP +N  VVAD VTE  F
Sbjct: 24  AAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPL-SPSVNDVVVADFVTEEFF 83

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F++A +SFD FGRIGSVDDSKREIAAFF HVT+E+G+ C
Sbjct: 84  NGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHFC 143

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEI+G +GDYCD+E TQYPCNPSK Y+GRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV
Sbjct: 144 FIEEINGPSGDYCDEENTQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 203

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A+DP VSF+TALWYWM+ V  VI+QGFGATIRA+NGA+ECDGGN   VQRR+  YTQYC+
Sbjct: 204 ATDPVVSFKTALWYWMSFVRPVINQGFGATIRAINGALECDGGNSATVQRRIQFYTQYCN 263

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 264 QLGVAPGDNLTC 274

BLAST of Lag0025149 vs. NCBI nr
Match: XP_038894874.1 (endochitinase EP3-like [Benincasa hispida])

HSP 1 Score: 400.2 bits (1027), Expect = 1.7e-107
Identity = 185/252 (73.41%), Postives = 203/252 (80.56%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG GCREGPC   P  +P +N  VVAD VTE  F
Sbjct: 25  AAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 84

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA  SFD FGRIGSVDDSKREIAAFF HVT+E+G+LC
Sbjct: 85  NGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 144

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE IGFDGLNNPEIV
Sbjct: 145 FIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIV 204

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A+DP VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDG N   VQRRV  YTQYCD
Sbjct: 205 ATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNATVQRRVQFYTQYCD 264

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG+NLTC
Sbjct: 265 QLGVAPGNNLTC 275

BLAST of Lag0025149 vs. NCBI nr
Match: XP_023001456.1 (endochitinase EP3 [Cucurbita maxima])

HSP 1 Score: 399.8 bits (1026), Expect = 2.2e-107
Identity = 184/252 (73.02%), Postives = 205/252 (81.35%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG GCREGPC   P  +P +N  VVAD VTE  F
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA +SFD FGRIGSVDDSKREIAAFF HVT+E+G+LC
Sbjct: 78  NGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A++P VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDGGN   VQRRV  YTQYC+
Sbjct: 198 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGGNSATVQRRVEYYTQYCN 257

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

BLAST of Lag0025149 vs. NCBI nr
Match: XP_023520063.1 (endochitinase EP3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 398.3 bits (1022), Expect = 6.5e-107
Identity = 183/252 (72.62%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG GD +CG GCREGPC   P  +P +N  VVAD VTE  F
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGDDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA +SFD FGRIGSVDDSKREIAAFF HVT+E+G+LC
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD+E TQYPCNP+K YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPNKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A++P VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDG N   VQRRV  YTQYC+
Sbjct: 198 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVEYYTQYCN 257

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

BLAST of Lag0025149 vs. NCBI nr
Match: KAG7019501.1 (Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 396.7 bits (1018), Expect = 1.9e-106
Identity = 182/252 (72.22%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG GCREGPC   P  +P +N  VVAD VTE  F
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA +SFD FGRIGSVDDSKREIAAFF HVT+E+G+LC
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD+E TQYPCNP+K YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPNKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A++P VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDG N   VQRRV  YTQYC+
Sbjct: 198 ATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVQYYTQYCN 257

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

BLAST of Lag0025149 vs. ExPASy Swiss-Prot
Match: Q9M2U5 (Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 4.9e-97
Identity = 162/250 (64.80%), Postives = 189/250 (75.60%), Query Frame = 0

Query: 80  APKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLFEN 139
           A  CGCS+ LCCS+ GFCG    YCG GC++GPC A P      NG  VA+IVT+  F  
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPP----ANGVSVAEIVTQEFFNG 87

Query: 140 ITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLCFV 199
           I       CAG RFYSR AF+EA +S+  FGR+GS DDS+REIAAFF+HVT+E+G+ C++
Sbjct: 88  IISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYI 147

Query: 200 EEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVAS 259
           EEIDG + DYCD+  TQYPCNP+K Y+GRGPIQLSWNFNYGPAG AIGFDGLN PE VA+
Sbjct: 148 EEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVAT 207

Query: 260 DPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCDQL 319
           DP +SF+TALWYW N V  VI QGFGATIRA+NGA+ECDG N   VQ RV  YT YC QL
Sbjct: 208 DPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQL 267

Query: 320 GVAPGHNLTC 330
           GV PG+NLTC
Sbjct: 268 GVDPGNNLTC 273

BLAST of Lag0025149 vs. ExPASy Swiss-Prot
Match: P27054 (Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.4e-90
Identity = 155/252 (61.51%), Postives = 187/252 (74.21%), Query Frame = 0

Query: 80  APKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPC-KAAPAPSPGMNGTVVADIVTEGLFE 139
           A  CGC+ GLCCS++G+CG G+ YCG GC++GPC  A+P PS  +N    ADI+T     
Sbjct: 23  AQNCGCAEGLCCSQYGYCGTGEDYCGTGCQQGPCTTASPPPSNNVN----ADILTADFLN 82

Query: 140 NITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLCF 199
            I    D  CAGK FY+R AF+ A  S+ +FGR+GS DDSKREIAA F+H T+E+G+ C+
Sbjct: 83  GIIDQADSGCAGKNFYTRDAFLSALNSYTDFGRVGSEDDSKREIAAAFAHFTHETGHFCY 142

Query: 200 VEEIDGDAGDYCDKEK-TQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 259
           +EEIDG + DYCD+E   QYPC+ SK Y GRGPIQLSWNFNYGPAG A  FDGL  PE V
Sbjct: 143 IEEIDGASKDYCDEESIAQYPCSSSKGYHGRGPIQLSWNFNYGPAGSANNFDGLGAPETV 202

Query: 260 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 319
           ++D  VSF+TALWYWM +V  VI+QGFGATIRA+NGA+ECDG NP  VQ RV  YT+YC 
Sbjct: 203 SNDVVVSFKTALWYWMQHVRPVINQGFGATIRAINGALECDGANPTTVQARVNYYTEYCR 262

Query: 320 QLGVAPGHNLTC 330
           QLGVA G NLTC
Sbjct: 263 QLGVATGDNLTC 270

BLAST of Lag0025149 vs. ExPASy Swiss-Prot
Match: Q7Y1Z0 (Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.0e-86
Identity = 144/262 (54.96%), Postives = 181/262 (69.08%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPC----------KAAPAPSPGMNGTV 137
           A A  CGC + +CCS+ G+CG G  YCG GCR GPC                  G +G  
Sbjct: 27  ASAQNCGCQSNMCCSKWGYCGTGKDYCGDGCRSGPCYGGGGGGGGGGGGGGGGGGGSGVS 86

Query: 138 VADIVTEGLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFS 197
           V  +VTE  F  I       CAGK FY+R++F+ AA S+  F R  + DDSKREIAAFF+
Sbjct: 87  VESVVTEAFFNGIKNQAPNGCAGKNFYTRQSFLNAAHSYSGFARDRTNDDSKREIAAFFA 146

Query: 198 HVTYESGNLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIG 257
           HVT+E+G++C++ EI+G + DYCDK   Q+PC P K+Y+GRGP+Q+SWN+NYGPAG+ IG
Sbjct: 147 HVTHETGHMCYINEINGASMDYCDKNNKQWPCQPGKKYYGRGPLQISWNYNYGPAGQNIG 206

Query: 258 FDGLNNPEIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQR 317
           FDGL +P+ VA DP +SF+TALW+WMNNVH V+ QGFGATIRA+NGA+EC+G NPGAV  
Sbjct: 207 FDGLRDPDRVAQDPTISFKTALWFWMNNVHQVMLQGFGATIRAINGALECNGKNPGAVNA 266

Query: 318 RVMLYTQYCDQLGVAPGHNLTC 330
           RV  Y  YC Q GV PG NL C
Sbjct: 267 RVNYYKDYCRQFGVDPGGNLYC 288

BLAST of Lag0025149 vs. ExPASy Swiss-Prot
Match: Q6K8R2 (Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.0e-86
Identity = 145/263 (55.13%), Postives = 179/263 (68.06%), Query Frame = 0

Query: 69  LISDTENEIAGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVV 128
           +++      A A  CGC++  CCS+ GFCG G  YCG GC+ GPC       P  N   V
Sbjct: 14  MVAAVSASTAAAQSCGCASDQCCSKWGFCGTGSDYCGTGCQAGPCDV-----PATNDVSV 73

Query: 129 ADIVTEGLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSH 188
           A IVT   F  +    D+ CA K FY+R AF+ AA  +  FGR GSVDDSKREIAAFF+H
Sbjct: 74  ASIVTPEFFAALVAQADDGCAAKGFYTRDAFLTAAGGYPSFGRTGSVDDSKREIAAFFAH 133

Query: 189 VTYESGNLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGF 248
             +E+   C++EEIDG + +YCD+  TQ+PC   K Y+GRGP+Q+SWNFNYGPAG++IGF
Sbjct: 134 ANHETIKFCYIEEIDGPSKNYCDETSTQWPCMAGKGYYGRGPLQISWNFNYGPAGQSIGF 193

Query: 249 DGLNNPEIVASDPEVSFETALWYWMNNVHS--VIDQGFGATIRAVNGAVECDGGNPGAVQ 308
           DGL +P+ VA  P ++F+TALWYW NNVH   V  QGFGATIRA+NGA+ECDG NP AV 
Sbjct: 194 DGLGDPDAVARSPVLAFQTALWYWTNNVHDAFVSGQGFGATIRAINGALECDGKNPTAVS 253

Query: 309 RRVMLYTQYCDQLGVAPGHNLTC 330
            RV  Y Q+C Q GV PG NLTC
Sbjct: 254 NRVAYYQQFCQQFGVDPGSNLTC 271

BLAST of Lag0025149 vs. ExPASy Swiss-Prot
Match: O04138 (Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2)

HSP 1 Score: 318.2 bits (814), Expect = 1.1e-85
Identity = 145/261 (55.56%), Postives = 180/261 (68.97%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPC----KAAPAPSPGMN-----GTVV 137
           A A  CGC  G CCS+ G+CG  + YCG GC+ GPC      A A   G       G  V
Sbjct: 25  AAAQNCGCQDGYCCSQWGYCGTTEAYCGQGCQSGPCWGSGGEAAAGMAGRKAGAGAGVSV 84

Query: 138 ADIVTEGLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSH 197
             +VTE  F  I       CAGK FY+R++F+ AA S+  F    + DDSKREIAAFF+H
Sbjct: 85  ESVVTEAFFNGIKNQAPNGCAGKSFYTRQSFLNAARSYSGFANDRTNDDSKREIAAFFAH 144

Query: 198 VTYESGNLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGF 257
           VT+E+G++C++ EI+G   DYCDK   Q+PC P K+Y+GRGP+Q+SWNFNYGPAG+ IGF
Sbjct: 145 VTHETGHMCYINEINGANMDYCDKSNKQWPCQPGKKYYGRGPLQISWNFNYGPAGKNIGF 204

Query: 258 DGLNNPEIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRR 317
           DGL +P+ VA DP +SF+TALW+WMNNVH V+ QGFGATIRA+NGA+EC+G NPGAV  R
Sbjct: 205 DGLRDPDKVAQDPTISFKTALWFWMNNVHQVMSQGFGATIRAINGALECNGKNPGAVNAR 264

Query: 318 VMLYTQYCDQLGVAPGHNLTC 330
           V  Y  YC Q GV+PG NL C
Sbjct: 265 VNYYKDYCRQFGVSPGGNLYC 285

BLAST of Lag0025149 vs. ExPASy TrEMBL
Match: A0A6J1CFV8 (endochitinase EP3-like OS=Momordica charantia OX=3673 GN=LOC111010839 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.9e-108
Identity = 182/252 (72.22%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG+GCREGPC   P  SP +N  VVAD VTE  F
Sbjct: 24  AAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPL-SPSVNDVVVADFVTEEFF 83

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F++A +SFD FGRIGSVDDSKREIAAFF HVT+E+G+ C
Sbjct: 84  NGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHFC 143

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEI+G +GDYCD+E TQYPCNPSK Y+GRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV
Sbjct: 144 FIEEINGPSGDYCDEENTQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 203

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A+DP VSF+TALWYWM+ V  VI+QGFGATIRA+NGA+ECDGGN   VQRR+  YTQYC+
Sbjct: 204 ATDPVVSFKTALWYWMSFVRPVINQGFGATIRAINGALECDGGNSATVQRRIQFYTQYCN 263

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 264 QLGVAPGDNLTC 274

BLAST of Lag0025149 vs. ExPASy TrEMBL
Match: A0A6J1KIN5 (endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 1.1e-107
Identity = 184/252 (73.02%), Postives = 205/252 (81.35%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG GCREGPC   P  +P +N  VVAD VTE  F
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA +SFD FGRIGSVDDSKREIAAFF HVT+E+G+LC
Sbjct: 78  NGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A++P VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDGGN   VQRRV  YTQYC+
Sbjct: 198 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGGNSATVQRRVEYYTQYCN 257

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

BLAST of Lag0025149 vs. ExPASy TrEMBL
Match: A0A6J1EGZ6 (endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.6e-106
Identity = 182/252 (72.22%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG GCREGPC   P  +P +N  VVAD VTE  F
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA +SFD FGRIGSVDDSKREIAAFF HVT+E+G+LC
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD++ TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEDNTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A++P VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDG N   VQRRV  YTQYC+
Sbjct: 198 ATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVEYYTQYCN 257

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

BLAST of Lag0025149 vs. ExPASy TrEMBL
Match: A0A0A0LXT8 (Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G534750 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 5.6e-104
Identity = 177/252 (70.24%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+AGLCCSR GFCG G+ +CG GCREGPC   P  +P +N   VA+ VTE  F
Sbjct: 28  AAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPL-TPSVNDVNVAEFVTEEFF 87

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAG+ FYSR  F+EA +SFD FGRIGSV+DSKREIAAFF+HVT+E+G+LC
Sbjct: 88  NGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKREIAAFFAHVTHETGHLC 147

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG   DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE IGFDGLNNPEIV
Sbjct: 148 FIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIV 207

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A++P VSF+TALWYWMN V  VI+QGFGATIRA+NGA+ECDG N   VQRR+  Y QYC+
Sbjct: 208 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANTATVQRRIQFYNQYCN 267

Query: 318 QLGVAPGHNLTC 330
           QL VAPG+NLTC
Sbjct: 268 QLNVAPGNNLTC 278

BLAST of Lag0025149 vs. ExPASy TrEMBL
Match: A0A077GZM8 (Chitinase ppl-2 OS=Pyrus x bretschneideri OX=225117 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 9.5e-104
Identity = 172/252 (68.25%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 78  AGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLF 137
           A A  CGC+A LCCS++G+CG  D YCG GC+ GPCK  PA +P  N  VVADIVT   F
Sbjct: 22  ADAQNCGCAADLCCSQYGYCGTDDAYCGKGCQSGPCK-TPAQTPSTNDVVVADIVTPEFF 81

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLC 197
             I    D  CAGK FYSR  F+EA  S+++FGRIGSVDDSKREIAAFF+HVT+E+G+ C
Sbjct: 82  NGIIDQADASCAGKNFYSRATFLEALNSYNQFGRIGSVDDSKREIAAFFAHVTHETGHFC 141

Query: 198 FVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           ++EEIDG + DYCD+  TQYPCNP+K Y+GRGPIQLSWNFNYGPAGE+IGFDGLN+PE V
Sbjct: 142 YIEEIDGASKDYCDENNTQYPCNPNKGYYGRGPIQLSWNFNYGPAGESIGFDGLNSPETV 201

Query: 258 ASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCD 317
           A+DP ++F+TALWYWMNNV  VI +GFGATIRA+NGA+ECDGGNP  VQ+RV  YT+YC+
Sbjct: 202 ANDPVIAFKTALWYWMNNVRPVIGEGFGATIRAINGALECDGGNPATVQKRVEYYTEYCN 261

Query: 318 QLGVAPGHNLTC 330
           QLGVAPG NLTC
Sbjct: 262 QLGVAPGDNLTC 272

BLAST of Lag0025149 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 355.9 bits (912), Expect = 3.5e-98
Identity = 162/250 (64.80%), Postives = 189/250 (75.60%), Query Frame = 0

Query: 80  APKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTEGLFEN 139
           A  CGCS+ LCCS+ GFCG    YCG GC++GPC A P      NG  VA+IVT+  F  
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPP----ANGVSVAEIVTQEFFNG 87

Query: 140 ITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLCFV 199
           I       CAG RFYSR AF+EA +S+  FGR+GS DDS+REIAAFF+HVT+E+G+ C++
Sbjct: 88  IISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYI 147

Query: 200 EEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVAS 259
           EEIDG + DYCD+  TQYPCNP+K Y+GRGPIQLSWNFNYGPAG AIGFDGLN PE VA+
Sbjct: 148 EEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVAT 207

Query: 260 DPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQYCDQL 319
           DP +SF+TALWYW N V  VI QGFGATIRA+NGA+ECDG N   VQ RV  YT YC QL
Sbjct: 208 DPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQL 267

Query: 320 GVAPGHNLTC 330
           GV PG+NLTC
Sbjct: 268 GVDPGNNLTC 273

BLAST of Lag0025149 vs. TAIR 10
Match: AT2G43590.1 (Chitinase family protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.5e-80
Identity = 134/255 (52.55%), Postives = 172/255 (67.45%), Query Frame = 0

Query: 75  NEIAGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTE 134
           +E   +  CGC+  LCCS+ G+CG  D YCG GCR GPC+ +  P+ G     V  IVT+
Sbjct: 19  SETVKSQNCGCAPNLCCSQFGYCGTDDAYCGVGCRSGPCRGSGTPTGGS----VGSIVTQ 78

Query: 135 GLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESG 194
           G F NI       CAGKRFY+R +FV AA +F  F        ++REIA  F+H T+E+G
Sbjct: 79  GFFNNIINQAGNGCAGKRFYTRDSFVNAANTFPNFAN----SVTRREIATMFAHFTHETG 138

Query: 195 NLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNP 254
           + C++EEI+G   +YC    TQYPC P K YFGRGPIQLSWN+NYG  G+++G D L  P
Sbjct: 139 HFCYIEEINGATRNYCQSSNTQYPCAPGKGYFGRGPIQLSWNYNYGACGQSLGLDLLRQP 198

Query: 255 EIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQ 314
           E+V S+P V+F T LW+WMN+V  V++QGFGATIRA+NG +EC+GGN GAV  R+  Y  
Sbjct: 199 ELVGSNPTVAFRTGLWFWMNSVRPVLNQGFGATIRAING-MECNGGNSGAVNARIGYYRD 258

Query: 315 YCDQLGVAPGHNLTC 330
           YC QLGV PG NL+C
Sbjct: 259 YCGQLGVDPGPNLSC 264

BLAST of Lag0025149 vs. TAIR 10
Match: AT2G43580.1 (Chitinase family protein )

HSP 1 Score: 261.9 bits (668), Expect = 6.8e-70
Identity = 119/255 (46.67%), Postives = 164/255 (64.31%), Query Frame = 0

Query: 75  NEIAGAPKCGCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGTVVADIVTE 134
           +E   +  C C+  LCCS+ G+CG    YCG+ C+ GPC+    P+      +V +IVT+
Sbjct: 19  SETVKSQNCDCAPNLCCSQFGYCGTTADYCGSTCQSGPCRVGGPPT---GAGLVGNIVTQ 78

Query: 135 GLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESG 194
             F NI       CAGK FY+R +F+ A  +F  F        ++REIA  F+H TYE+G
Sbjct: 79  IFFNNIINQAGNGCAGKSFYTRDSFINATNTFPSFAN----TVTRREIATMFAHFTYETG 138

Query: 195 NLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNP 254
           + C++EEI+G +   CD+   QYPC P+K Y GRGP+ LSWNFNYG  G+++G D L  P
Sbjct: 139 HFCYIEEINGASRVMCDQNNRQYPCAPAKSYHGRGPLLLSWNFNYGACGQSLGLDLLRQP 198

Query: 255 EIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQ 314
           E+V+S+P V+F TALW+WM +V  V++QGFGATIRA++G  +CDG N G V  R+  Y  
Sbjct: 199 ELVSSNPVVAFRTALWFWMKSVRPVLNQGFGATIRAISG-FDCDGRNLGGVNARIGYYRD 258

Query: 315 YCDQLGVAPGHNLTC 330
           YC QLG+ PG N+TC
Sbjct: 259 YCGQLGLDPGANITC 265

BLAST of Lag0025149 vs. TAIR 10
Match: AT2G43620.1 (Chitinase family protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.6e-66
Identity = 122/257 (47.47%), Postives = 164/257 (63.81%), Query Frame = 0

Query: 84  GCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAA-----PAPSPGMNG------TVVADIV 143
           GC+A LCCSR+G+CG  D YCG GCR GPC ++     P PS G  G        + ++V
Sbjct: 35  GCAANLCCSRYGYCGTTDAYCGTGCRSGPCSSSTTPIPPTPSGGAGGLNADPRDTIENVV 94

Query: 144 TEGLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYE 203
           T   F+ I       C  K FY+R+AF+ AA+SFD +   G+V  +KREIAA  +  ++E
Sbjct: 95  TPAFFDGIMSKVGNGCPAKGFYTRQAFIAAAQSFDAYK--GTV--AKREIAAMLAQFSHE 154

Query: 204 SGNLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLN 263
           SG+ C+ EEI    G YC    T YPC P K Y+GRGPIQ++WN+NYG AG+ +G   L 
Sbjct: 155 SGSFCYKEEI--ARGKYC-SPSTAYPCTPGKDYYGRGPIQITWNYNYGAAGKFLGLPLLT 214

Query: 264 NPEIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLY 323
           +P++VA  P+V+F+ A+W+W  NV  V+DQGFGAT R +NG  EC+G  P AVQ RV  Y
Sbjct: 215 DPDMVARSPQVAFQCAMWFWNLNVRPVLDQGFGATTRKINGG-ECNGRRPAAVQSRVNYY 274

Query: 324 TQYCDQLGVAPGHNLTC 330
            ++C  LG+ PG NL+C
Sbjct: 275 LEFCRTLGITPGANLSC 283

BLAST of Lag0025149 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.9e-65
Identity = 118/255 (46.27%), Postives = 159/255 (62.35%), Query Frame = 0

Query: 84  GCSAGLCCSRHGFCGVGDGYCGAGCREGPCKAAPAPSPGMNGT---------VVADIVTE 143
           GC   +CCSR G+CG    YCG GC+ GPC + P P+P  +G+          +A ++T 
Sbjct: 35  GCKGNMCCSRWGYCGTTKAYCGTGCQSGPCNSKPKPTPTPSGSGGLNAGPRGTIASVITP 94

Query: 144 GLFENITGGGDERCAGKRFYSRKAFVEAAESFDEFGRIGSVDDSKREIAAFFSHVTYESG 203
             F +I       C  K FY+R+AF+ AAESF  +   G+V  +KREIAA  +  ++ESG
Sbjct: 95  AFFNSIMSKVGSGCPAKGFYTRQAFIAAAESFAAYK--GTV--AKREIAAMLAQFSHESG 154

Query: 204 NLCFVEEIDGDAGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNP 263
           + C+ EEI    G YC    T YPC P K Y+GRGPIQ++WN+NYG AG+ +G   L +P
Sbjct: 155 SFCYKEEI--ARGRYC-SPSTTYPCQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDP 214

Query: 264 EIVASDPEVSFETALWYWMNNVHSVIDQGFGATIRAVNGAVECDGGNPGAVQRRVMLYTQ 323
           ++VA  P V+F+ A+W+W  NV  V+ QGFGAT R +NG  EC+GG P AVQ RV  Y  
Sbjct: 215 DMVARSPTVAFQCAMWFWNKNVRPVLSQGFGATTRRINGG-ECNGGRPAAVQSRVNHYLD 274

Query: 324 YCDQLGVAPGHNLTC 330
           +C +LGV PG NL+C
Sbjct: 275 FCKKLGVTPGTNLSC 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140102.15.9e-10872.22endochitinase EP3-like [Momordica charantia][more]
XP_038894874.11.7e-10773.41endochitinase EP3-like [Benincasa hispida][more]
XP_023001456.12.2e-10773.02endochitinase EP3 [Cucurbita maxima][more]
XP_023520063.16.5e-10772.62endochitinase EP3 [Cucurbita pepo subsp. pepo][more]
KAG7019501.11.9e-10672.22Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9M2U54.9e-9764.80Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1[more]
P270544.4e-9061.51Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1[more]
Q7Y1Z01.0e-8654.96Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1[more]
Q6K8R25.0e-8655.13Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1[more]
O041381.1e-8555.56Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CFV82.9e-10872.22endochitinase EP3-like OS=Momordica charantia OX=3673 GN=LOC111010839 PE=4 SV=1[more]
A0A6J1KIN51.1e-10773.02endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1[more]
A0A6J1EGZ61.6e-10672.22endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1[more]
A0A0A0LXT85.6e-10470.24Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A077GZM89.5e-10468.25Chitinase ppl-2 OS=Pyrus x bretschneideri OX=225117 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G54420.13.5e-9864.80homolog of carrot EP3-3 chitinase [more]
AT2G43590.11.5e-8052.55Chitinase family protein [more]
AT2G43580.16.8e-7046.67Chitinase family protein [more]
AT2G43620.11.6e-6647.47Chitinase family protein [more]
AT2G43610.13.9e-6546.27Chitinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001002Chitin-binding, type 1SMARTSM00270ChitinBD_3coord: 82..113
e-value: 7.3E-8
score: 42.1
coord: 32..61
e-value: 1.8
score: 9.2
IPR001002Chitin-binding, type 1PROSITEPS50941CHIT_BIND_I_2coord: 80..115
score: 8.996321
NoneNo IPR availableGENE3D1.10.530.10coord: 126..329
e-value: 9.9E-52
score: 176.9
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 200..257
e-value: 1.4E-26
score: 94.1
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 18..61
NoneNo IPR availablePANTHERPTHR22595:SF131ENDOCHITINASE EP3coord: 75..329
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 75..329
NoneNo IPR availablePANTHERPTHR22595:SF131ENDOCHITINASE EP3coord: 18..61
NoneNo IPR availableCDDcd00035ChtBD1coord: 85..108
e-value: 1.16814E-5
score: 39.6762
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 132..329
e-value: 3.46294E-80
score: 241.565
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 7..78
e-value: 0.019
score: 12.3
coord: 62..329
e-value: 1.9E-94
score: 314.4
IPR036861Endochitinase-like superfamilyGENE3D3.30.60.10coord: 78..115
e-value: 2.0E-7
score: 32.9
IPR036861Endochitinase-like superfamilySUPERFAMILY57016Plant lectins/antimicrobial peptidescoord: 33..65
IPR036861Endochitinase-like superfamilySUPERFAMILY57016Plant lectins/antimicrobial peptidescoord: 80..116
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 131..275
e-value: 1.7E-41
score: 142.5
coord: 283..329
e-value: 8.2E-8
score: 32.3
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00773CHITINASE_19_1coord: 148..170
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 263..273
IPR018371Chitin-binding, type 1, conserved sitePROSITEPS00026CHIT_BIND_I_1coord: 85..104
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 128..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0025149.1Lag0025149.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0006952 defense response
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding