Lag0024925 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0024925
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	BHLH domain-containing protein
Location	chr10: 7066016 .. 7066601 (-)
RNA-Seq Expression	Lag0024925
Synteny	Lag0024925

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAACATCCCAATAATCCATTCTCCATCATCAGCAAGAACCGACCGAAAATTCATAGAGAGAAATAGAAGAAACCAAATGAAGACCCTTTTATCCACGCTCCATTCTCTTGTCCCCAATCAAAGCTCAAGGGTTTGATCTTTTCTTTTTTCTCTCCTGTTTGGTTGATGAGAAAATTATATCATTTTGATGAGAGTTATTGGTGCAGGAAGAAGGTGCTAAAACGGTGCCGGATCAGCTAGAAAACGCCACAAATTACATAAAAGAACTGCAGACGAAGGTGGAGAAGTTGAAAGAGAAGAGAGAGAAGCTGCTGGGAATTAGGGGAAGAGAAAAAGAAAGAATAATTACAACTACAACAGCATGCAGTGAAGAAATTAAACAGAGATTATCATTGCTGCAACTTAAAACTCATCAAGTGGGTTCTTCCATGGAGATCCTTTTGACCACTGGATCCGATTATCACTTTGTTCTCACACAGATCCTTCGCCTTCTTCAACAAAATGGTGCTGAAATCTTCAATGTCAATCAGTCCATGTTCCCAGATCGGATTTTTCACAAGATCATAGCTCAGGTAATGTAA

mRNA sequence

ATGGCGAACATCCCAATAATCCATTCTCCATCATCAGCAAGAACCGACCGAAAATTCATAGAGAGAAATAGAAGAAACCAAATGAAGACCCTTTTATCCACGCTCCATTCTCTTGTCCCCAATCAAAGCTCAAGGGAAGAAGGTGCTAAAACGGTGCCGGATCAGCTAGAAAACGCCACAAATTACATAAAAGAACTGCAGACGAAGGTGGAGAAGTTGAAAGAGAAGAGAGAGAAGCTGCTGGGAATTAGGGGAAGAGAAAAAGAAAGAATAATTACAACTACAACAGCATGCAGTGAAGAAATTAAACAGAGATTATCATTGCTGCAACTTAAAACTCATCAAGTGGGTTCTTCCATGGAGATCCTTTTGACCACTGGATCCGATTATCACTTTGTTCTCACACAGATCCTTCGCCTTCTTCAACAAAATGGTGCTGAAATCTTCAATGTCAATCAGTCCATGTTCCCAGATCGGATTTTTCACAAGATCATAGCTCAGGTAATGTAA

Coding sequence (CDS)

ATGGCGAACATCCCAATAATCCATTCTCCATCATCAGCAAGAACCGACCGAAAATTCATAGAGAGAAATAGAAGAAACCAAATGAAGACCCTTTTATCCACGCTCCATTCTCTTGTCCCCAATCAAAGCTCAAGGGAAGAAGGTGCTAAAACGGTGCCGGATCAGCTAGAAAACGCCACAAATTACATAAAAGAACTGCAGACGAAGGTGGAGAAGTTGAAAGAGAAGAGAGAGAAGCTGCTGGGAATTAGGGGAAGAGAAAAAGAAAGAATAATTACAACTACAACAGCATGCAGTGAAGAAATTAAACAGAGATTATCATTGCTGCAACTTAAAACTCATCAAGTGGGTTCTTCCATGGAGATCCTTTTGACCACTGGATCCGATTATCACTTTGTTCTCACACAGATCCTTCGCCTTCTTCAACAAAATGGTGCTGAAATCTTCAATGTCAATCAGTCCATGTTCCCAGATCGGATTTTTCACAAGATCATAGCTCAGGTAATGTAA

Protein sequence

MANIPIIHSPSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKELQTKVEKLKEKREKLLGIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTTGSDYHFVLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQVM

Homology

[Close]

BLAST of Lag0024925 vs. NCBI nr
Match: XP_008442634.2 (PREDICTED: transcription factor bHLH118-like [Cucumis melo])

HSP 1 Score: 180.3 bits (456), Expect = 1.4e-41
Identity = 100/171 (58.48%), Postives = 125/171 (73.10%), Query Frame = 0


Query: 7   IHSPSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKEL 66
           I    + ++DRKF+ERNRR +MK+L STL+SL+PN +SR E  +TVPDQLE+ATNYIKEL
Sbjct: 5   IRCQPTPQSDRKFVERNRRKEMKSLFSTLNSLLPNHTSR-EAPRTVPDQLEDATNYIKEL 64

Query: 67  QTKVEKLKEKREKLLG---------IRGREKERIITTTTACSEEIKQRLSLLQLKTHQVG 126
           Q  ++KLKEK+E+L+G           GR + R          E K +L LLQ+K HQ+G
Sbjct: 65  QKNIQKLKEKKEELMGKNKMEEDEEAEGRRRRRF-------EHETKPKL-LLQVKAHQIG 124

Query: 127 SSMEILLTTGSDYHFVLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQV 169
           SS+E+ LTTGSDYHF+L Q+LRLLQ NGAEI NVNQSMF DR+FHKI AQV
Sbjct: 125 SSVEVFLTTGSDYHFILQQVLRLLQDNGAEILNVNQSMFTDRVFHKITAQV 166

[Close]

BLAST of Lag0024925 vs. NCBI nr
Match: XP_004146605.1 (transcription factor bHLH162 isoform X1 [Cucumis sativus] >KGN64684.1 hypothetical protein Csa_013539 [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 7.1e-41
Identity = 96/162 (59.26%), Postives = 122/162 (75.31%), Query Frame = 0


Query: 7   IHSPSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKEL 66
           I    + +++RKF+ERNRR +MK L STL+SL+PNQ+S  E  +TVPDQLE+ATNYIKEL
Sbjct: 5   IRCQPTPQSNRKFVERNRRKEMKALFSTLNSLLPNQTSM-EAPRTVPDQLEDATNYIKEL 64

Query: 67  QTKVEKLKEKREKLLGIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTT 126
           Q  ++KLKEK+EKL+G+   E+           +E K +LS + +K HQ+GSS+E+ LTT
Sbjct: 65  QKNIKKLKEKKEKLMGMEEDEEAEGRRRRRGYEDETKPKLS-VHVKAHQIGSSVEVFLTT 124

Query: 127 GSDYHFVLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQV 169
           GSDYHF L Q+LRLLQ NGAEI NVNQSMF DR+FHKI AQV
Sbjct: 125 GSDYHFNLQQVLRLLQDNGAEILNVNQSMFTDRVFHKITAQV 164

[Close]

BLAST of Lag0024925 vs. NCBI nr
Match: XP_031737224.1 (transcription factor bHLH162 isoform X3 [Cucumis sativus])

HSP 1 Score: 177.6 bits (449), Expect = 9.3e-41
Identity = 95/163 (58.28%), Postives = 123/163 (75.46%), Query Frame = 0


Query: 7   IHSPSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKEL 66
           I    + +++RKF+ERNRR +MK L STL+SL+PNQ+S  E  +TVPDQLE+ATNYIKEL
Sbjct: 5   IRCQPTPQSNRKFVERNRRKEMKALFSTLNSLLPNQTSM-EAPRTVPDQLEDATNYIKEL 64

Query: 67  QTKVEKLKEKREKLLGIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTT 126
           Q  ++KLKEK+EKL+G+   E+           +E K +LS + +K HQ+GSS+E+ LTT
Sbjct: 65  QKNIKKLKEKKEKLMGMEEDEEAEGRRRRRGYEDETKPKLS-VHVKAHQIGSSVEVFLTT 124

Query: 127 GSDYHFVLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQVM 170
           GSDYHF L Q+LRLLQ NGAEI NVNQSMF DR+FHKI AQ++
Sbjct: 125 GSDYHFNLQQVLRLLQDNGAEILNVNQSMFTDRVFHKITAQLL 165

[Close]

BLAST of Lag0024925 vs. NCBI nr
Match: XP_031737221.1 (transcription factor bHLH162 isoform X2 [Cucumis sativus])

HSP 1 Score: 176.4 bits (446), Expect = 2.1e-40
Identity = 95/161 (59.01%), Postives = 121/161 (75.16%), Query Frame = 0


Query: 7   IHSPSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKEL 66
           I    + +++RKF+ERNRR +MK L STL+SL+PNQ+S  E  +TVPDQLE+ATNYIKEL
Sbjct: 5   IRCQPTPQSNRKFVERNRRKEMKALFSTLNSLLPNQTSM-EAPRTVPDQLEDATNYIKEL 64

Query: 67  QTKVEKLKEKREKLLGIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTT 126
           Q  ++KLKEK+EKL+G+   E+           +E K +LS + +K HQ+GSS+E+ LTT
Sbjct: 65  QKNIKKLKEKKEKLMGMEEDEEAEGRRRRRGYEDETKPKLS-VHVKAHQIGSSVEVFLTT 124

Query: 127 GSDYHFVLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQ 168
           GSDYHF L Q+LRLLQ NGAEI NVNQSMF DR+FHKI AQ
Sbjct: 125 GSDYHFNLQQVLRLLQDNGAEILNVNQSMFTDRVFHKITAQ 163

[Close]

BLAST of Lag0024925 vs. NCBI nr
Match: XP_023542172.1 (transcription factor bHLH167-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176.0 bits (445), Expect = 2.7e-40
Identity = 104/162 (64.20%), Postives = 122/162 (75.31%), Query Frame = 0


Query: 7   IHSPSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKEL 66
           I+ PSS RTD K IERNRR +MK LLS LHSLVPNQSS  EG  T+ DQLENATNYIK+L
Sbjct: 6   IYCPSSTRTDEKIIERNRREEMKALLSELHSLVPNQSS-TEGEMTLSDQLENATNYIKQL 65

Query: 67  QTKVEKLKEKREKLLGIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTT 126
           +  VEKLKEKREKL+G+   EK  I       + E K RL +LQ++ H+VGSS+EILLTT
Sbjct: 66  KENVEKLKEKREKLMGL--GEKSTI-------TSENKARL-ILQVEAHRVGSSLEILLTT 125

Query: 127 GSDYHFVLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQV 169
           GS YH VL QI++LLQ+NG EI ++NQS   DR FHKIIAQV
Sbjct: 126 GSVYHLVLRQIIQLLQENGTEIVDINQSTVADRAFHKIIAQV 156

[Close]

BLAST of Lag0024925 vs. ExPASy Swiss-Prot
Match: F4JIJ7 (Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.9e-18
Identity = 59/159 (37.11%), Postives = 95/159 (59.75%), Query Frame = 0


Query: 12  SARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKELQTKVE 71
           S   DRK +E+NRR QMK+L S L SL+P+ SS E    T+PDQL+ A NYIK+LQ  VE
Sbjct: 11  SRSVDRKTVEKNRRMQMKSLYSELISLLPHHSSTE--PLTLPDQLDEAANYIKKLQVNVE 70

Query: 72  KLKEKREKLLGIRGREK-ERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTTGSDY 131
           K +E++  L+     EK   + +++ + S ++     L +++  + GS   I L T  ++
Sbjct: 71  KKRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVTSLEH 130

Query: 132 HFVLTQILRLL-QQNGAEIFNVNQSMFPDRIFHKIIAQV 169
            F+  +I+R+L ++ GAEI +   S+  D +FH +  +V
Sbjct: 131 KFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKV 167

[Close]

BLAST of Lag0024925 vs. ExPASy Swiss-Prot
Match: Q9STJ6 (Transcription factor bHLH126 OS=Arabidopsis thaliana OX=3702 GN=BHLH126 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-05
Identity = 36/149 (24.16%), Postives = 74/149 (49.66%), Query Frame = 0


Query: 20  IERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKELQTKVEKLKEKREK 79
           IER RR +M TL +TL + +P +  +  G + V D +  A N+IK+ + ++++L  +R++
Sbjct: 50  IERQRRQEMATLFATLRTHLPLKYIK--GKRAVSDHVNGAVNFIKDTEARIKELSARRDE 109

Query: 80  LLGIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEILLTTGSDYHFVLTQILR 139
           L    G+  +         S+  K   + + ++ H  G  + +   +       L+++L 
Sbjct: 110 LSRETGQGYKSNPDPGKTGSDVGKSEPATVMVQPHVSGLEVVVSSNSSGPEALPLSKVLE 169

Query: 140 LLQQNGAEIFNVNQSMFPDRIFHKIIAQV 169
            +Q+ G E+ +   +   DR+ H I  +V
Sbjct: 170 TIQEKGLEVMSSFTTRVNDRLMHTIQVEV 196

[Close]

BLAST of Lag0024925 vs. ExPASy Swiss-Prot
Match: Q9FLI1 (Transcription factor bHLH36 OS=Arabidopsis thaliana OX=3702 GN=BHLH36 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.7e-05
Identity = 45/156 (28.85%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 21  ERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKELQTKVEKLKEKREKL 80
           ER RR +M +L ++L SL+P    +  G ++  DQ+  A NYIK LQ K+++L  +R+ L
Sbjct: 10  ERQRRQEMASLYASLRSLLPLHFIK--GKRSTSDQVNEAVNYIKYLQRKIKELSVRRDDL 69

Query: 81  LGI-RGREKERIITTTTACSEEIKQRLSLLQLKTH----QVGSSMEILLTT---GSDYHF 140
           + + RG       +   + + + K+ + ++  K H    Q    +EI+L++   G    F
Sbjct: 70  MVLSRG-------SLLGSSNGDFKEDVEMISGKNHVVVRQCLVGVEIMLSSRCCGGQPRF 129

Query: 141 VLTQILRLLQQNGAEIFNVNQSMFPDRIFHKIIAQV 169
             + +L++L + G  + N   S+  DR+ + I A+V
Sbjct: 130 --SSVLQVLSEYGLCLLNSISSIVDDRLVYTIQAEV 154

[Close]

BLAST of Lag0024925 vs. ExPASy Swiss-Prot
Match: Q9LQ08 (Transcription factor bHLH125 OS=Arabidopsis thaliana OX=3702 GN=BHLH125 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.2e-04
Identity = 31/98 (31.63%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 12  SARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKELQTKVE 71
           S +   + IER RR ++ +L   L +L+P Q    +G ++  D +  A NYIK+LQ K++
Sbjct: 73  SKKMKHRDIERQRRQEVSSLFKRLRTLLPFQYI--QGKRSTSDHIVQAVNYIKDLQIKIK 132

Query: 72  KLKEKREKLLGIRGREKERIITTTTACSEEIKQRLSLL 110
           +L EKR ++        +++I+ TT     I++  S L
Sbjct: 133 ELNEKRNRV--------KKVISATTTTHSAIEECTSSL 160

[Close]

BLAST of Lag0024925 vs. ExPASy Swiss-Prot
Match: Q9LN95 (Transcription factor bHLH55 OS=Arabidopsis thaliana OX=3702 GN=BHLH55 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 9.4e-04
Identity = 41/148 (27.70%), Postives = 80/148 (54.05%), Query Frame = 0


Query: 10  PSSARTDRKFIERNRRNQMKTLLSTLHSLVPNQSSREEGAKTVPDQLENATNYIKELQTK 69
           P + R   K +ER RR +  +L   L  L+P+Q  +  G ++  D +  A NYIK+LQ K
Sbjct: 72  PKNKRAKHKELERQRRQENTSLFKILRYLLPSQYIK--GKRSSADHVLEAVNYIKDLQKK 131

Query: 70  VEKLKEKREKLL------GIRGREKERIITTTTACSEEIKQRLSLLQLKTHQVGSSMEIL 129
           ++++ EKR+++         RG    R + ++T CS      ++++ ++   +G  +EI+
Sbjct: 132 IKEVSEKRDRIKRSITHPSSRGEFSIRSLASST-CSCVGDTNIAVV-VRPCLIG--LEIV 191

Query: 130 LTTGSDYHFVLTQILRLLQQNGAEIFNV 152
           ++  + +   L+ +L+LL Q   + FN+
Sbjct: 192 VSCCNRHESCLSSVLQLLAQE--QCFNI 211

The following BLAST results are available for this feature:

BLAST of Lag0024925 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442634.2	1.4e-41	58.48	PREDICTED: transcription factor bHLH118-like [Cucumis melo]	[more]
XP_004146605.1	7.1e-41	59.26	transcription factor bHLH162 isoform X1 [Cucumis sativus] >KGN64684.1 hypothetic...	[more]
XP_031737224.1	9.3e-41	58.28	transcription factor bHLH162 isoform X3 [Cucumis sativus]	[more]
XP_031737221.1	2.1e-40	59.01	transcription factor bHLH162 isoform X2 [Cucumis sativus]	[more]
XP_023542172.1	2.7e-40	64.20	transcription factor bHLH167-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0024925 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIJ7	3.9e-18	37.11	Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=...	[more]
Q9STJ6	1.0e-05	24.16	Transcription factor bHLH126 OS=Arabidopsis thaliana OX=3702 GN=BHLH126 PE=2 SV=...	[more]
Q9FLI1	1.7e-05	28.85	Transcription factor bHLH36 OS=Arabidopsis thaliana OX=3702 GN=BHLH36 PE=2 SV=1	[more]
Q9LQ08	3.2e-04	31.63	Transcription factor bHLH125 OS=Arabidopsis thaliana OX=3702 GN=BHLH125 PE=2 SV=...	[more]
Q9LN95	9.4e-04	27.70	Transcription factor bHLH55 OS=Arabidopsis thaliana OX=3702 GN=BHLH55 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 56..83
None	No IPR available	PANTHER	PTHR13935:SF90	TRANSCRIPTION FACTOR BHLH162	coord: 8..168
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 18..72 e-value: 0.0024 score: 27.1
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 14..67 e-value: 1.4E-7 score: 31.4
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 12..66 score: 11.274042
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 7..81 e-value: 5.9E-11 score: 44.1
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 10..80
IPR015660	Achaete-scute transcription factor-related	PANTHER	PTHR13935	ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED	coord: 8..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0024925.1	Lag0024925.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0090575	RNA polymerase II transcription regulator complex
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000977	RNA polymerase II transcription regulatory region sequence-specific DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity