Lag0024572 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024572
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily
Locationchr10: 4065755 .. 4066363 (-)
RNA-Seq ExpressionLag0024572
SyntenyLag0024572
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGCAAAAACGGTTATTGTGGATAACAGGTGACACGGGTTTTGGAACCATGGAATCCTCCACTTTCGGTAAGCATGAGCAACCCAACCTTTTCTTTTGTCTTCTCTTTTGATTTGTTCATTTCAAAAATATTAATTAATTATTGGTCTATGAGTCGAGTGAAAAATCTTCCTTCTTTGTTTATTTTTTATTTTTTAGTTAGCTAATTAGATAATGGAAAATATGTGTTAATATAGTGAGTCAATACTTGTAATTCCTTTTTTTCATTGATTAAAAAAATGCCTAGTTTCTTAAAAAGTACTTTTACTACCTATTATCTTATCTCTTTTAATATTGATATTGTGCATTATGATTTGAATATTTTGAATCAATTGAATGGAATGACAGTATTTGAGGATGGTTAACTTGGTCGAGTCATCTAAATTTTTTATTCTATGTAGAATTTAACAATAGAGGTAAAGAAGCTAATTTAAGTGGAAGAGTCAAGTGGCCAGGATTTCCTGTGCTAAACAATCCAGTACAAGCCACTAATTTTACCTTTAGATTTTTCATTGATGGAAACACATGGTTACCTGAGACGGGTGTACCATATAGGCCAGAATTATAG

mRNA sequence

ATGTCGCAAAAACGGTTATTGTGGATAACAGGTGACACGGGTTTTGGAACCATGGAATCCTCCACTTTCGAATTTAACAATAGAGGTAAAGAAGCTAATTTAAGTGGAAGAGTCAAGTGGCCAGGATTTCCTGTGCTAAACAATCCAGTACAAGCCACTAATTTTACCTTTAGATTTTTCATTGATGGAAACACATGGTTACCTGAGACGGGTGTACCATATAGGCCAGAATTATAG

Coding sequence (CDS)

ATGTCGCAAAAACGGTTATTGTGGATAACAGGTGACACGGGTTTTGGAACCATGGAATCCTCCACTTTCGAATTTAACAATAGAGGTAAAGAAGCTAATTTAAGTGGAAGAGTCAAGTGGCCAGGATTTCCTGTGCTAAACAATCCAGTACAAGCCACTAATTTTACCTTTAGATTTTTCATTGATGGAAACACATGGTTACCTGAGACGGGTGTACCATATAGGCCAGAATTATAG

Protein sequence

MSQKRLLWITGDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPETGVPYRPEL
Homology
BLAST of Lag0024572 vs. NCBI nr
Match: KAG7019877.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 87.4 bits (215), Expect = 5.8e-14
Identity = 37/55 (67.27%), Postives = 44/55 (80.00%), Query Frame = 0

Query: 24  EFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPETGVPYRPEL 79
           E++NRG  ANLSGRVKWPGF  + N  +AT F+ R FI+G TWLPETGVPYRP+L
Sbjct: 336 EYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGVPYRPDL 390

BLAST of Lag0024572 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 87.0 bits (214), Expect = 7.6e-14
Identity = 37/55 (67.27%), Postives = 43/55 (78.18%), Query Frame = 0

Query: 24  EFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPETGVPYRPEL 79
           E+ NRG  ANLSGRVKWPGF  + N  +AT F+ R FI+G TWLPETGVPYRP+L
Sbjct: 281 EYGNRGPGANLSGRVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

BLAST of Lag0024572 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 87.0 bits (214), Expect = 7.6e-14
Identity = 37/55 (67.27%), Postives = 43/55 (78.18%), Query Frame = 0

Query: 24  EFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPETGVPYRPEL 79
           E+ NRG  ANLSGRVKWPGF  + N  +AT F+ R FI+G TWLPETGVPYRP+L
Sbjct: 281 EYGNRGPGANLSGRVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

BLAST of Lag0024572 vs. NCBI nr
Match: XP_022137308.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 84.0 bits (206), Expect = 6.4e-13
Identity = 33/55 (60.00%), Postives = 44/55 (80.00%), Query Frame = 0

Query: 24  EFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPETGVPYRPEL 79
           EFNNRG  A++S R+KW G+ ++NNP++AT +T + FIDG TWLPET VP+RP L
Sbjct: 261 EFNNRGPGADISKRIKWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

BLAST of Lag0024572 vs. NCBI nr
Match: KAF3339285.1 (pectinesterase-like protein [Carex littledalei])

HSP 1 Score: 79.3 bits (194), Expect = 1.6e-11
Identity = 37/67 (55.22%), Postives = 41/67 (61.19%), Query Frame = 0

Query: 8   WITGDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWL 67
           W+  D  FG       EFNN G  A L+ RVKWPGF VL  P QATNFT   FI+GN WL
Sbjct: 390 WLEWDGDFGLKTLYYAEFNNTGPGAALANRVKWPGFHVLTRPRQATNFTVGHFINGNVWL 449

Query: 68  PETGVPY 75
           P TGV +
Sbjct: 450 PRTGVAF 456

BLAST of Lag0024572 vs. ExPASy Swiss-Prot
Match: Q84JX1 (Probable pectinesterase/pectinesterase inhibitor 19 OS=Arabidopsis thaliana OX=3702 GN=PME19 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.7e-11
Identity = 33/68 (48.53%), Postives = 40/68 (58.82%), Query Frame = 0

Query: 11  GDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPET 70
           G+TG  T+     E+ NRG  A  S RVKW GF V+ +P QAT FT    +DG TWL ET
Sbjct: 459 GETGLSTLHYR--EYQNRGPGAVTSRRVKWSGFKVMKDPKQATEFTVAKLLDGETWLKET 518

Query: 71  GVPYRPEL 79
            +PY   L
Sbjct: 519 RIPYESGL 524

BLAST of Lag0024572 vs. ExPASy Swiss-Prot
Match: Q1PEC0 (Probable pectinesterase/pectinesterase inhibitor 42 OS=Arabidopsis thaliana OX=3702 GN=PME42 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 6.3e-11
Identity = 32/68 (47.06%), Postives = 41/68 (60.29%), Query Frame = 0

Query: 11  GDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWLPET 70
           G+TG  T+     E+ NRG  A  S RVKW GF V+ +P QAT FT    +DG TWL E+
Sbjct: 459 GETGLSTLHYR--EYQNRGPGAVTSRRVKWSGFKVMKDPKQATEFTVAKLLDGETWLKES 518

Query: 71  GVPYRPEL 79
            +PY+  L
Sbjct: 519 RIPYKSGL 524

BLAST of Lag0024572 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 6.3e-11
Identity = 31/70 (44.29%), Postives = 37/70 (52.86%), Query Frame = 0

Query: 8   WITGDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWL 67
           W+  +  F        E+ N G  A L  RVKWPG+ V N   QA N+T   FI+GN WL
Sbjct: 450 WLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWL 509

Query: 68  PETGVPYRPE 78
           P TGV Y  E
Sbjct: 510 PSTGVKYTAE 519

BLAST of Lag0024572 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 8.2e-11
Identity = 32/67 (47.76%), Postives = 37/67 (55.22%), Query Frame = 0

Query: 8   WITGDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWL 67
           W+  +  F        EF N G  + LS RVKWPG+ V NN  QA NFT   FI GN WL
Sbjct: 453 WLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGNLWL 512

Query: 68  PETGVPY 75
           P TGV +
Sbjct: 513 PSTGVTF 519

BLAST of Lag0024572 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.4e-10
Identity = 29/67 (43.28%), Postives = 38/67 (56.72%), Query Frame = 0

Query: 8   WITGDTGFGTMESSTFEFNNRGKEANLSGRVKWPGFPVLNNPVQATNFTFRFFIDGNTWL 67
           W+ G   F        E+ N G  AN++ RV WPGF  + N  +AT FT   FIDG+TWL
Sbjct: 462 WLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWL 521

Query: 68  PETGVPY 75
             TG+P+
Sbjct: 522 NSTGIPF 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019877.15.8e-1467.27putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp... [more]
KAG6584281.17.6e-1467.27putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp... [more]
KAG6584282.17.6e-1467.27putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp... [more]
XP_022137308.16.4e-1360.00probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia][more]
KAF3339285.11.6e-1155.22pectinesterase-like protein [Carex littledalei][more]
Match NameE-valueIdentityDescription
Q84JX13.7e-1148.53Probable pectinesterase/pectinesterase inhibitor 19 OS=Arabidopsis thaliana OX=3... [more]
Q1PEC06.3e-1147.06Probable pectinesterase/pectinesterase inhibitor 42 OS=Arabidopsis thaliana OX=3... [more]
Q430626.3e-1144.29Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
Q9SMY78.2e-1147.76Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
O814152.4e-1043.28Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 22..64
e-value: 4.9E-8
score: 32.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 4..78
e-value: 2.4E-10
score: 42.2
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 23..75
NoneNo IPR availablePANTHERPTHR31707:SF188PECTINESTERASEcoord: 23..75
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 7..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024572.1Lag0024572.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity