Lag0024300 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0024300 vs. NCBI nr
Match: XP_038894367.1 (LOW QUALITY PROTEIN: LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Benincasa hispida])

HSP 1 Score: 889.8 bits (2298), Expect = 1.4e-254
Identity = 469/681 (68.87%), Postives = 526/681 (77.24%), Query Frame = 0

Query: 1   MKISLNPIISNFFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPES 60
           MKI L P  S FFI FI +HLSPCF+E ALD  + CG SYNCG LVNI  PFWG ++ +S
Sbjct: 1   MKIYLKPRNSTFFIIFIAIHLSPCFNEGALDAFEVCGESYNCGELVNIKYPFWGNKKQKS 60

Query: 61  CGWKEFHLKCKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATL 120
           CG +EF L C+ N+TTTI INS+EY VLKINQSNN MRIARSDLF+NYCP+N+IQ  AT+
Sbjct: 61  CGRQEFKLNCRKNQTTTIYINSVEYNVLKINQSNNGMRIARSDLFENYCPENEIQ-VATM 120

Query: 121 GEHL-------------------------RRFR-----RVTNLG--------AVGKRNLQ 180
             HL                         +R++     +   LG        +   RN++
Sbjct: 121 DAHLFAYASNNQNISVWYNCSGENYIPTPKRYKFWCSGKWGKLGRANYAFESSAANRNME 180

Query: 181 MEECTMNIEVTVAMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGA 240
             ECTMNIEV V ++ L+EG+K   SLVE AVK GFDVEYG W+KEAC+ C ESGGKCG 
Sbjct: 181 KGECTMNIEVMVTIDLLKEGIKGGKSLVEKAVKWGFDVEYGNWYKEACNECNESGGKCGG 240

Query: 241 NGTNPFYCICGNGVANPYFFCKAAMLP------ASKSN---AMTGFGSGIGGILLMSLIF 300
           N T PFYCIC NGVANPY  CKAA+ P       S  N    + G GSG GGI++ SLIF
Sbjct: 241 NKTYPFYCICKNGVANPY-DCKAALPPPALKIKGSNDNWKKVVIGVGSGFGGIVITSLIF 300

Query: 301 FIRLRLHKKKHLRA-SSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPAN 360
           FIRLRL+KKKH RA SS LL+NNS D   KELE GENY+ VPLFS+ ELVQATDKFNPAN
Sbjct: 301 FIRLRLNKKKHFRASSSILLQNNSRDRLMKELEKGENYITVPLFSYQELVQATDKFNPAN 360

Query: 361 ELGDGGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTS 420
           ELGDG FGTVYYGKLRDGREVAVKRLFEN+ +KV+HF+NEVEILTRL HPHLVTLYG TS
Sbjct: 361 ELGDGSFGTVYYGKLRDGREVAVKRLFENNVKKVEHFLNEVEILTRLCHPHLVTLYGCTS 420

Query: 421 RRSRELLLVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRD 480
           RRSRELLLVYEF+ NGTVADHLHG +ARP ELPW TRLKIAIETASALAFLHAS+ IHRD
Sbjct: 421 RRSRELLLVYEFVPNGTVADHLHGIQARPGELPWLTRLKIAIETASALAFLHASKTIHRD 480

Query: 481 VKTTNILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVF 540
           VKTTNIL+DNNFNVKVADFGLSRLFPTQATH+STAPQGTPGY+DPEY+ECYQLT+KSDVF
Sbjct: 481 VKTTNILVDNNFNVKVADFGLSRLFPTQATHVSTAPQGTPGYIDPEYYECYQLTSKSDVF 540

Query: 541 SFGVVLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVI 600
           SFGVVLVELISSKPAVDITRHR EINLSTMA+NKIQN+EL +FVDP LGF+TD+R+RD+I
Sbjct: 541 SFGVVLVELISSKPAVDITRHRDEINLSTMAMNKIQNDELDDFVDPCLGFKTDKRIRDMI 600

Query: 601 WAVAELAFRCLQSVRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVP 634
             VAELAF+CLQSVRDTRPSMLEALE LKNIESRSCGK K                    
Sbjct: 601 CRVAELAFQCLQSVRDTRPSMLEALEILKNIESRSCGKEKG------------------- 660

BLAST of Lag0024300 vs. NCBI nr
Match: XP_023519142.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 878.2 bits (2268), Expect = 4.1e-251
Identity = 456/667 (68.37%), Postives = 505/667 (75.71%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCK 71
           FF  F T HLSPCF+++ALD  K CGV+YNCG LVNIT PFWG +R  SCG KEF L C 
Sbjct: 6   FFFFFFTFHLSPCFTDNALDAFKDCGVNYNCGELVNITYPFWGNKRQSSCGRKEFKLNCN 65

Query: 72  HNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQ-------------RTA 131
           +NRTTT  INS+EY VL+INQ NN MRIARSDLFDNYCP+NQIQ             R  
Sbjct: 66  NNRTTTTYINSLEYNVLEINQLNNTMRIARSDLFDNYCPRNQIQVASMDHHPFAYSARNQ 125

Query: 132 TL----------------------GEHLRRFRRVTNLGAVGKRNLQMEECTMNIEVTVAM 191
            +                      GE  RR R             ++ EC M IEV V +
Sbjct: 126 NISVGYNCSPDNEFPVSEYNFSCGGEWERRGRVNYGFEPSAAVYSKLVECEMKIEVMVTI 185

Query: 192 EGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNGV 251
           +GL+EGVKN+ SLVETAVK GF+VEY  W+K ACD C  SGG CG NGT PFYCIC NGV
Sbjct: 186 KGLKEGVKNRRSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNGV 245

Query: 252 ANPYFFCKAAMLPA----------SKSNAMTGFGSGIGGILLMSLIFFIRLRLHKKKHLR 311
           ANPY  C A   P           +    + G GSGIGGI++MS+IFFIRLR+HKKKH  
Sbjct: 246 ANPY-ACDATAAPPRPPHPENPNDTWKKVVIGAGSGIGGIVMMSIIFFIRLRMHKKKH-- 305

Query: 312 ASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGTVYYGK 371
                 +N SCD P KELE  E+YM + LFS+HELV+ATDKFNPANELGDGGFGTVYYGK
Sbjct: 306 ------QNYSCDRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDGGFGTVYYGK 365

Query: 372 LRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFIS 431
           LRDGREVAVKRLFEN+YRKV+ FMNEVEILTRLRHPHLVTLYG TSRRSRELLLVYEFI 
Sbjct: 366 LRDGREVAVKRLFENNYRKVEQFMNEVEILTRLRHPHLVTLYGCTSRRSRELLLVYEFIP 425

Query: 432 NGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFNV 491
           NGTVADHLHG RA+P ELPWHTRLKIAIETASALAFLHASE IHRDVKTTNILLDNNF V
Sbjct: 426 NGTVADHLHGERAKPGELPWHTRLKIAIETASALAFLHASETIHRDVKTTNILLDNNFGV 485

Query: 492 KVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKP 551
           KVADFGL RLFPTQA+H+STAPQGTPGY+DPEY+E YQ+TNKSDVFSFGVVLVELISSKP
Sbjct: 486 KVADFGLCRLFPTQASHVSTAPQGTPGYIDPEYYESYQVTNKSDVFSFGVVLVELISSKP 545

Query: 552 AVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQSV 611
           AVDITRHRHEINLSTMAINKIQN+ELH+FVDPSLGF+ DQR+RD+I  VAELAF+CLQSV
Sbjct: 546 AVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKIDQRIRDMICGVAELAFQCLQSV 605

Query: 612 RDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISKS 634
           RDTRPSM+EALE L+NIE+RSCG GKA                LVP+SP+SV V WISK+
Sbjct: 606 RDTRPSMMEALEILRNIENRSCGTGKAP---------------LVPESPDSVTVLWISKT 648

BLAST of Lag0024300 vs. NCBI nr
Match: XP_022137292.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111008789 [Momordica charantia])

HSP 1 Score: 877.5 bits (2266), Expect = 7.0e-251
Identity = 461/674 (68.40%), Postives = 512/674 (75.96%), Query Frame = 0

Query: 1   MKISLNPIISNFFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPES 60
           MK S +  ISN F  FI  HLS CF+  A+DE KACG SYNCG LVNI  PFWG ER   
Sbjct: 1   MKTSQSTTISNVF--FIAFHLSLCFTVDAIDEFKACGHSYNCGELVNIAYPFWGNERKGF 60

Query: 61  CGWKEFHLKCKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATL 120
           CG +EF LKCKHNRTTTI+INS+EY +LKINQS++RM IARSDLFDN CP+NQ Q TATL
Sbjct: 61  CGRREFELKCKHNRTTTIQINSLEYNILKINQSDHRMTIARSDLFDNLCPKNQTQ-TATL 120

Query: 121 GEHLRRFRRVTNLGAVGKR--------------------------------------NLQ 180
             HL  F   +N   +  R                                      NL 
Sbjct: 121 DHHL--FVYSSNDQNISVRYNCSAQKEIPETYKFSCGSEEEKNGRANYAFEPSAATWNLL 180

Query: 181 MEECTMNIEVTVAMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGA 240
           +EECTMNI+V V MEGL+EG+KN+  LVE AV+ GFDVEYG  +  AC+ C+ +GG CG 
Sbjct: 181 IEECTMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGG 240

Query: 241 NGTNPFYCICGNGVANPYFFCKAAMLPASK---SNAMTGFGSGIGGILLMSLIFFIRLRL 300
           NGT PFYCIC +G  +PYF   A  L  S     N + G G GIGGI+LM  I  I LRL
Sbjct: 241 NGTYPFYCICRSGEIHPYFCGTAHALEESNDKWKNTVIGVGCGIGGIILMGTISLILLRL 300

Query: 301 HKKKHLRASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGF 360
            K KH  ASSF L NN  DPP+KELE GENYM VPLFS+ EL +ATDKFNPA ELGDGGF
Sbjct: 301 QKNKHAHASSFPLGNNYFDPPSKELEKGENYMVVPLFSYGELEEATDKFNPAKELGDGGF 360

Query: 361 GTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELL 420
           GTVYYGKLRDGREVAVKRLFEN+YRKV+HFMNEV+ILT L H HLVTLYG TSRRSRELL
Sbjct: 361 GTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRELL 420

Query: 421 LVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNIL 480
           LVYEFI NGT+ADHLHGNRA+  ELPW TRLKIAIETASALA+LHAS+ IHRDVKTTNIL
Sbjct: 421 LVYEFIPNGTLADHLHGNRAKSGELPWITRLKIAIETASALAYLHASKTIHRDVKTTNIL 480

Query: 481 LDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLV 540
           LD N +VKVAD GLS L PTQATH+STAPQGTPGY+DPEY ECYQLTNKSDVFSFGVVLV
Sbjct: 481 LDKNLSVKVADLGLSCLSPTQATHVSTAPQGTPGYIDPEYQECYQLTNKSDVFSFGVVLV 540

Query: 541 ELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELA 600
           ELISSKPAVD+TRHRHEINL TMAINKIQNN+LHNFVDPSL FETDQ VRD+I+AVA LA
Sbjct: 541 ELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAVAGLA 600

Query: 601 FRCLQSVRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVI 634
           F+CLQSV+D RPSMLEALE LKNIESRSCG+ K EE+ I  +D+  LK G VPQSP+SV 
Sbjct: 601 FQCLQSVKDMRPSMLEALEILKNIESRSCGRRKTEELDILSEDE--LKVGSVPQSPDSVT 660

BLAST of Lag0024300 vs. NCBI nr
Match: XP_038894039.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 [Benincasa hispida])

HSP 1 Score: 874.8 bits (2259), Expect = 4.6e-250
Identity = 455/677 (67.21%), Postives = 519/677 (76.66%), Query Frame = 0

Query: 1   MKISLNPIISN-FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPE 60
           MK S+   +S+ FF + I   L  CF++ A +E KACGV YNCG LVNI+ PFWG ER  
Sbjct: 1   MKTSVLLFLSSFFFFTIICFDLQLCFADDAHEEFKACGVYYNCGDLVNISYPFWGNERQN 60

Query: 61  SCGWKEFHLKCKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTAT 120
            CG +EF LKCK N+TTTI+INSM + V+ I++S+++M IARSDLFD+YCP N+I+   T
Sbjct: 61  FCGRREFELKCKDNKTTTIQINSMVFNVVNISKSDHKMTIARSDLFDDYCPNNRIEEVTT 120

Query: 121 LGEHL-------------------------RRFRRVTNLGAVGKRNLQME---------- 180
           L   L                          RF   +     G+ N  +E          
Sbjct: 121 LDYKLFKYSSNDLNLSVWYDCPPLEGPLGDFRFSCGSERERSGRENYALEMKDAMYWRRD 180

Query: 181 --ECTMNIEVTVAMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGA 240
             +C ++IEVT+ ME L+EG  N+  LVE   K GF+VEYG ++  ACDGCKE GGKCG 
Sbjct: 181 MSDCELHIEVTITMEVLKEGATNRTILVERGTKEGFEVEYGDFYTVACDGCKEYGGKCGG 240

Query: 241 NGTNPFYCICGNGVANPYFFCKAAMLPASK---SNAMTGFGSGIGGILLMSLIFFIRLRL 300
           N T+ FYCICGNG  +PY        P        A+ G   GIGG+LLMS+ FFI   L
Sbjct: 241 NATHEFYCICGNGDIHPYVCRPPPPAPGESKKWKKAVIGVCIGIGGMLLMSVAFFIWFCL 300

Query: 301 HKKKHLRA---SSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGD 360
           HKKK  R+   SSFLLRN SC+P TKELE GEN MG+PLFS+ EL +ATD+FNPA ELGD
Sbjct: 301 HKKKLARSYTPSSFLLRNISCEPSTKELEKGENDMGLPLFSYEELEKATDRFNPAKELGD 360

Query: 361 GGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSR 420
           GGFGTVYYGKL DGREVAVKRLFEN+YR+V+HFMNEVEILTRLRHPHLVTLYG TSR  R
Sbjct: 361 GGFGTVYYGKLPDGREVAVKRLFENNYRRVEHFMNEVEILTRLRHPHLVTLYGCTSRHCR 420

Query: 421 ELLLVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTT 480
           ELLLVYEFI NGTVADHLHGNRA+P ELPWHTRLKIAI+TASALAFLHASE IHRDVKTT
Sbjct: 421 ELLLVYEFIPNGTVADHLHGNRAKPGELPWHTRLKIAIDTASALAFLHASETIHRDVKTT 480

Query: 481 NILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGV 540
           NILLDNN+NVKVADFGLSRLFPTQATH+STAPQGTPGYVDPEYHECYQLTNKSDVFSFGV
Sbjct: 481 NILLDNNYNVKVADFGLSRLFPTQATHVSTAPQGTPGYVDPEYHECYQLTNKSDVFSFGV 540

Query: 541 VLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVA 600
           VLVELISSKPAVDITRHRHEINL TMAINKI+N+ELH+FVD SLGFETD+ VRD+I AVA
Sbjct: 541 VLVELISSKPAVDITRHRHEINLWTMAINKIRNDELHDFVDSSLGFETDETVRDMICAVA 600

Query: 601 ELAFRCLQSVRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPN 634
           ELAFRCLQSV+DTRPSMLEALE LKNIESRS G+GK E+I ISG+DD LLK GLVP+SP+
Sbjct: 601 ELAFRCLQSVKDTRPSMLEALEILKNIESRSSGRGK-EDIDISGEDDMLLKDGLVPESPD 660

BLAST of Lag0024300 vs. NCBI nr
Match: XP_022923737.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cucurbita moschata])

HSP 1 Score: 869.4 bits (2245), Expect = 1.9e-248
Identity = 454/668 (67.96%), Postives = 505/668 (75.60%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCK 71
           FF  F T HLSPCFS++ALD  K CGV+YNC  LVNIT PFWG ER  SCG KEF L C 
Sbjct: 39  FFFFFFTFHLSPCFSDNALDAFKDCGVNYNCEELVNITYPFWGNERQSSCGRKEFKLNCN 98

Query: 72  HNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATLGEHLRRF-RRV 131
           +NRTTT  INS+EY VL+INQ NN MRIARSDLFDNYCP+NQIQ  A++  H   +  R 
Sbjct: 99  NNRTTTTYINSLEYNVLEINQLNNTMRIARSDLFDNYCPRNQIQ-VASMDHHPFVYSARN 158

Query: 132 TNLGA-------------------------VGKRNLQME----------ECTMNIEVTVA 191
            N+                            G+ N   E          EC M IEV V 
Sbjct: 159 QNISVGYNCSTDNEFPISEYNFSCGGEWERRGRVNYAFEPSAAVYSKLVECEMKIEVMVT 218

Query: 192 MEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNG 251
           ++GL+EGVKN+ SLVETAVK GF+VEY  W+K ACD C  SGG CG NGT PFYCIC NG
Sbjct: 219 IKGLKEGVKNRRSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNG 278

Query: 252 VANPYFFCKAAMLPA----------SKSNAMTGFGSGIGGILLMSLIFFIRLRLHKKKHL 311
           VANPY  C A   P           +    + G GSGIGGI++MS+IFFIRLR+HKKKH 
Sbjct: 279 VANPY-ACDATAAPPRPPHPENPNDTWKKVVIGAGSGIGGIVMMSIIFFIRLRMHKKKH- 338

Query: 312 RASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGTVYYG 371
                  +N SCD P KELE  E+YM + LFS+HELV+ATDKFNPANELGDGGFGTVYYG
Sbjct: 339 -------QNYSCDRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDGGFGTVYYG 398

Query: 372 KLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFI 431
           KLRDGREVAVKRLFEN+YRKV+ FMNEVE+LTRLRHPHLVTLYG TSRRSRELLLVYEFI
Sbjct: 399 KLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRELLLVYEFI 458

Query: 432 SNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFN 491
            NGTVADHLHGNRAR  ELPW TRL +AIETASAL+FLHASE IHRDVKTTNILLDNNF 
Sbjct: 459 PNGTVADHLHGNRARSGELPWLTRLNVAIETASALSFLHASETIHRDVKTTNILLDNNFG 518

Query: 492 VKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSK 551
           VKVADFGL RLFPTQA+H+STAPQGTPGY+DPEY+E YQLTNKSDVFSFGVVLVELISSK
Sbjct: 519 VKVADFGLCRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVVLVELISSK 578

Query: 552 PAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQS 611
           PAVDITRHRHEINLSTMAINKIQN+ELH+FVDPSLGF+TDQR+R++I  VAELAF+CLQS
Sbjct: 579 PAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAELAFQCLQS 638

Query: 612 VRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISK 634
            RDTRPSM+EALE L+NIE+RSCG GKA                LVP+SP+SV V WISK
Sbjct: 639 ARDTRPSMMEALEILRNIENRSCGTGKAP---------------LVPESPDSVTVLWISK 681

BLAST of Lag0024300 vs. ExPASy Swiss-Prot
Match: F4HQ17 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.4 PE=2 SV=2)

HSP 1 Score: 487.3 bits (1253), Expect = 2.7e-136
Identity = 296/667 (44.38%), Postives = 385/667 (57.72%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCK 71
           FFI F   +  PC       ES  C   + CG  +  + PFWG +R + CG     L+C 
Sbjct: 11  FFILFSLFYHLPC-------ESSKCESLFQCGN-ITASFPFWGGDRHKHCGHPLLELRCD 70

Query: 72  HNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRT--ATLGEHLRRFRR 131
            N++T++ I+  E++VL ++Q++  + +AR DL  ++C       T    + E    ++ 
Sbjct: 71  QNKSTSLFISDQEFFVLHVDQTSYSLTLARPDLLHSFCSLTFTNTTLPPEIFELSPAYKS 130

Query: 132 VT--------------NLGAVGKRNL-----QMEECTMNIEVTVAMEGLREGVKNKMSLV 191
           VT                  +G  ++       E C  N    V    + +  K  ++ +
Sbjct: 131 VTFYHCYPVLPDLSNYTCPVIGPISVSGNPEDHETCFPNFAANVPTSFVTKEKKLNIANL 190

Query: 192 ETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNGVANPYFF-CKAAMLP 251
           E+ ++ GF+V      K AC  C  S   CG +   PF   C      P+      ++  
Sbjct: 191 ESVLEKGFEVNMNVIMK-ACQACSYSNESCGFDENFPFEVKC-----KPHHSPTDTSLSI 250

Query: 252 ASKSNAMTGFGSGIGGILLMSLIFFIRLR------LHKKKHLRASSFLLR---------- 311
            +K+       SG+  +LL  L   IR R       +  K L  +S+  R          
Sbjct: 251 GAKAGIAVASVSGLAILLLAGLFLCIRRRRKTQDAQYTSKSLPITSYSSRDTSRNPTSTT 310

Query: 312 -----NNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGTVYYGKLR 371
                N+S  P    L N  +Y GV +FS+ EL +AT+ F  + ELGDGGFGTVYYG L+
Sbjct: 311 ISSSSNHSLLPSISNLANRSDYCGVQVFSYEELEEATENF--SRELGDGGFGTVYYGVLK 370

Query: 372 DGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFISNG 431
           DGR VAVKRL+E S ++V+ F NE+EIL  L+HP+LV LYG TSR SRELLLVYE+ISNG
Sbjct: 371 DGRAVAVKRLYERSLKRVEQFKNEIEILKSLKHPNLVILYGCTSRHSRELLLVYEYISNG 430

Query: 432 TVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFNVKV 491
           T+A+HLHGNRA    L W TRL IAIETASAL+FLH   IIHRD+KTTNILLD+N+ VKV
Sbjct: 431 TLAEHLHGNRAEARPLCWSTRLNIAIETASALSFLHIKGIIHRDIKTTNILLDDNYQVKV 490

Query: 492 ADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPAV 551
           ADFGLSRLFP   THISTAPQGTPGYVDPEY++CYQL  KSDV+SFGVVL ELISSK AV
Sbjct: 491 ADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYQLNEKSDVYSFGVVLTELISSKEAV 550

Query: 552 DITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQSVRD 611
           DITRHRH+INL+ MA++KIQNN LH  VD SLG++ D  VR  + AVAELAFRCLQ  RD
Sbjct: 551 DITRHRHDINLANMAVSKIQNNALHELVDSSLGYDNDPEVRRKMMAVAELAFRCLQQERD 610

Query: 612 TRPSMLEALETLKNI---ESRSCGKGKAEEIGI---SGDDDGLLKGGLVPQ-SPNSVIVP 629
            RP+M E +E L+ I   E +       + + I    GDD GLL+  + P  SP +    
Sbjct: 611 VRPAMDEIVEILRGIKDDEKKRVLVKSPDVVDIECGGGDDVGLLRNSVPPPISPET--DK 659

BLAST of Lag0024300 vs. ExPASy Swiss-Prot
Match: Q8VYG0 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.3 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.3 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.9e-134
Identity = 291/659 (44.16%), Postives = 392/659 (59.48%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKC- 71
           FF+S+I  H  PC    A  + + C   + CG L     PFWG  RP+ CG     L C 
Sbjct: 20  FFLSYI--HFLPC----AQSQREPCDTLFRCGDL-TAGFPFWGVARPQPCGHPSLGLHCQ 79

Query: 72  KHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTAT--LGEHLRRFR 131
           K   +T++ I+S+ Y VL++N + + +++ R D    +C  +    T T  L E L  ++
Sbjct: 80  KQTNSTSLIISSLMYRVLEVNTTTSTLKLVRQDFSGPFCSASFSGATLTPELFELLPDYK 139

Query: 132 RVT--------------------NLGAVGKRNLQMEECTMNIEVTVAMEGLREGVKNKMS 191
            ++                     +G++ + +L    C     +TV +    E     ++
Sbjct: 140 TLSAYYLCNPSLHYPAKFICPNKGVGSIHQDDLYHNHCGGIFNITVPIGYAPEEGALNVT 199

Query: 192 LVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNGVANPYFFCKAAML 251
            +E+ +K GF+V+     +  C  CK +GG C  +   P   +C    ++    C   M 
Sbjct: 200 NLESVLKKGFEVKL-SIDERPCQECKTNGGICAYHVATP---VCCKTNSSSEVNCTPMMP 259

Query: 252 PASKSNA--------MTGFGSG-IGGILLMSLIFFIRLRLHKKKHLR------------A 311
             S ++A          GF SG +G  L+   +  I +R  KK   +            +
Sbjct: 260 SGSSAHAGLSKKGKIGIGFASGFLGATLIGGCLLCIFIRRRKKLATQYTNKGLSTTTPYS 319

Query: 312 SSFLL-----------RNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGD 371
           S++ +            N+S  P    L NG  Y G+ +FS+ EL +AT+ F  + ELGD
Sbjct: 320 SNYTMSNTPTSTTISGSNHSLVPSISNLGNGSVYSGIQVFSYEELEEATENF--SKELGD 379

Query: 372 GGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSR 431
           GGFGTVYYG L+DGR VAVKRLFE S ++V+ F NE++IL  L+HP+LV LYG T+R SR
Sbjct: 380 GGFGTVYYGTLKDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSR 439

Query: 432 ELLLVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTT 491
           ELLLVYE+ISNGT+A+HLHGN+A+   + W  RL+IAIETASAL++LHAS IIHRDVKTT
Sbjct: 440 ELLLVYEYISNGTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTT 499

Query: 492 NILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGV 551
           NILLD+N+ VKVADFGLSRLFP   THISTAPQGTPGYVDPEY++CY+L  KSDV+SFGV
Sbjct: 500 NILLDSNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGV 559

Query: 552 VLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVA 611
           VL ELISSK AVDITRHRH+INL+ MAI+KIQN+ +H   D SLGF  D  V+ ++ +VA
Sbjct: 560 VLSELISSKEAVDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVA 619

Query: 612 ELAFRCLQSVRDTRPSMLEALETLKNIESR--SCGKGKAEEIGISGDDD-GLLKGGLVP 613
           ELAFRCLQ  RD RPSM E +E L+ I+    S  K    EI ++G DD GLLK G+ P
Sbjct: 620 ELAFRCLQQERDVRPSMDEIVEVLRVIQKDGISDSKDVVVEIDVNGGDDVGLLKHGVPP 665

BLAST of Lag0024300 vs. ExPASy Swiss-Prot
Match: P0C5E2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.2 PE=2 SV=3)

HSP 1 Score: 476.5 bits (1225), Expect = 4.7e-133
Identity = 255/465 (54.84%), Postives = 327/465 (70.32%), Query Frame = 0

Query: 167 MSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNGVANPYFFCKAA 226
           M+ VE  +K GF +    W   +C  C  SGG+CG +    F C+C +G    +  C   
Sbjct: 200 MTYVE-ILKRGFVL---NWTANSCFRCITSGGRCGTD-QQEFVCLCPDG-PKLHDTCTNG 259

Query: 227 MLPASKS---NAMTGFGSGIGGILLMSLIFFIRLRLHKKKHLRASSFLLRNNSCDPPTK- 286
                +      + G  + + G++  S+ +++  R   K +  +S+ L RN S DP  K 
Sbjct: 260 KNDKRRRVIVKVLIGASAAVVGLIAASIFWYVYHRRKTKSYRNSSALLPRNISSDPSAKS 319

Query: 287 -ELENGENYM-GVPLFSFHELVQATDKFNPANELGDGGFGTVYYGKLRDGREVAVKRLFE 346
            ++E  E  + GV +FS+ EL +AT+ F+P+ ELGDGGFGTVYYGKL+DGR VAVKRL++
Sbjct: 320 FDIEKAEELLVGVHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKRLYD 379

Query: 347 NSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFISNGTVADHLHGNRAR 406
           N++++ + F NEVEILT LRHP+LV L+G +S++SR+LLLVYE+++NGT+ADHLHG +A 
Sbjct: 380 NNFKRAEQFRNEVEILTGLRHPNLVALFGCSSKQSRDLLLVYEYVANGTLADHLHGPQAN 439

Query: 407 PSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFNVKVADFGLSRLFPTQ 466
           PS LPW  RLKIA+ETASAL +LHAS+IIHRDVK+ NILLD NFNVKVADFGLSRLFP  
Sbjct: 440 PSSLPWSIRLKIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLSRLFPMD 499

Query: 467 ATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPAVDITRHRHEINLS 526
            TH+STAPQGTPGYVDP+YH CYQL+NKSDV+SF VVL+ELISS PAVDITR R EINLS
Sbjct: 500 KTHVSTAPQGTPGYVDPDYHLCYQLSNKSDVYSFAVVLMELISSLPAVDITRPRQEINLS 559

Query: 527 TMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQSVRDTRPSMLEALETL 586
            MA+ KIQN+EL + VDPSLGF+TD RVR  + AVAELAF+CLQS +D RP M    +TL
Sbjct: 560 NMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMSHVQDTL 619

Query: 587 KNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISK 626
             I++   G     E+ +    D    G LV QSP+SVIV W SK
Sbjct: 620 TRIQNNGFG----SEMDVV---DVNKSGPLVAQSPDSVIVKWDSK 651

BLAST of Lag0024300 vs. ExPASy Swiss-Prot
Match: Q9C6K9 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.1 PE=2 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.3e-122
Identity = 249/454 (54.85%), Postives = 307/454 (67.62%), Query Frame = 0

Query: 190 CDGCKESGGKCGANGTNPFYCICGNGVANPYFFCKAAMLPASKSNAMTGFGSGIGG-ILL 249
           C  C  +GG+C     N + C+  N   N Y             +A    G GIGG ++L
Sbjct: 190 CFRCHNNGGEC-TKVKNNYRCVGANTEPNNY-------------HAEMRLGLGIGGSVIL 249

Query: 250 MSLIFFIRLRLHKKKHLRASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKF 309
           + ++  +   +H+    +  S L R+NS      ++E  + +  +P+FS+ EL  ATD F
Sbjct: 250 IIILVALFAVIHRNYRRKDGSELSRDNS----KSDVEFSQVFFKIPIFSYKELQAATDNF 309

Query: 310 NPANELGDGGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLY 369
           +    LGDGGFGTVYYGK+RDGREVAVKRL+E++YR+++ FMNE+EILTRL H +LV+LY
Sbjct: 310 SKDRLLGDGGFGTVYYGKVRDGREVAVKRLYEHNYRRLEQFMNEIEILTRLHHKNLVSLY 369

Query: 370 GSTSRRSRELLLVYEFISNGTVADHLHG-NRARPSELPWHTRLKIAIETASALAFLHASE 429
           G TSRRSRELLLVYEFI NGTVADHL+G N      L W  RL IAIETASALA+LHAS+
Sbjct: 370 GCTSRRSRELLLVYEFIPNGTVADHLYGENTPHQGFLTWSMRLSIAIETASALAYLHASD 429

Query: 430 IIHRDVKTTNILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTN 489
           IIHRDVKTTNILLD NF VKVADFGLSRL P+  TH+STAPQGTPGYVDPEYH CY LT+
Sbjct: 430 IIHRDVKTTNILLDRNFGVKVADFGLSRLLPSDVTHVSTAPQGTPGYVDPEYHRCYHLTD 489

Query: 490 KSDVFSFGVVLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQR 549
           KSDV+SFGVVLVELISSKPAVDI+R + EINLS++AINKIQN+  H  +D +LG+ T++ 
Sbjct: 490 KSDVYSFGVVLVELISSKPAVDISRCKSEINLSSLAINKIQNHATHELIDQNLGYATNEG 549

Query: 550 VRDVIWAVAELAFRCLQSVRDTRPSMLEALETLKNI--ESRSCGKGKAEEIGI----SGD 609
           VR +   VAELAF+CLQ     RP+M + +  LK I  E + C      E  I    S  
Sbjct: 550 VRKMTTMVAELAFQCLQQDNTMRPTMEQVVHELKGIQNEEQKCPTYDYREETIIPHPSPP 609

Query: 610 DDG---LLKGGLVPQSPNSVIVPWISKSSTPNGS 633
           D G   LLK    P+SP SV   W SKS+TPN S
Sbjct: 610 DWGEAALLKNMKFPRSPVSVTDQWTSKSTTPNTS 625

BLAST of Lag0024300 vs. ExPASy Swiss-Prot
Match: Q8RY67 (Wall-associated receptor kinase-like 14 OS=Arabidopsis thaliana OX=3702 GN=WAKL14 PE=2 SV=2)

HSP 1 Score: 288.1 bits (736), Expect = 2.4e-76
Identity = 200/588 (34.01%), Postives = 292/588 (49.66%), Query Frame = 0

Query: 33  SKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCKHNRTTTIEINSMEYYVLKINQ 92
           S  C + ++C A      P  G    ++       +   HN T  IE  +  +       
Sbjct: 64  SNGCSIRFDCSA---AEKPMIGDFSVQNVTENSIFVGLSHNCTRKIEDMNPLFGENFAPT 123

Query: 93  SNNRMRIARSDLFDNYCPQNQIQRTATLGEHLRRFRRVTNLGAVGKRNLQMEECTMNIEV 152
           S N   +   +   + C   Q        E++ + +     G +   +L     + N   
Sbjct: 124 SENSFLMENCNRTTDGCSIKQ-----KFLENVLKLKSCDATGNISCFSLDSNSSSKNSAK 183

Query: 153 TVAMEGLREG----VKNKMSLVETAVKHGFDVEY-----GKWFKEACDGCKESGGKCGAN 212
             +M+ LR      + + ++     V  G  +E+     G W K  C+      G C AN
Sbjct: 184 FFSMKTLRNSSCSLLFSSIAFESVGVNAGIALEFERVRLGWWLKGGCE-----SGTCAAN 243

Query: 213 ----------GTNPFYCIC-----GNGVANPYFFCKAAMLPA---------SKSNAMTGF 272
                     G     C C     G+G  NP   C+ A+             +SN +T  
Sbjct: 244 TDCTDVETPHGYAGHRCSCLDGFHGDGYTNP---CQRALPECRGSKLVWRHCRSNLITIV 303

Query: 273 GSGIGG-ILLMSLIFFIRLRLHKKKHLRASSFLLRNNSCDPPTKELENGENYMGVPLFSF 332
           G  +GG  LL +L FF   +  +   LR+     R          L        V  F +
Sbjct: 304 GGTVGGAFLLAALAFFFFCKRRRSTPLRSHLSAKR---------LLSEAAGNSSVAFFPY 363

Query: 333 HELVQATDKFNPANELGDGGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTR 392
            E+ +ATD F+   +LG G +GTVY GKL++   VA+KRL       +   MNE+++L+ 
Sbjct: 364 KEIEKATDGFSEKQKLGIGAYGTVYRGKLQNDEWVAIKRLRHRDSESLDQVMNEIKLLSS 423

Query: 393 LRHPHLVTLYGSTSRRSRELLLVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETAS 452
           + HP+LV L G    +  + +LVYE++ NGT+++HL   R R S LPW  RL +A +TA 
Sbjct: 424 VSHPNLVRLLGCCIEQG-DPVLVYEYMPNGTLSEHL--QRDRGSGLPWTLRLTVATQTAK 483

Query: 453 ALAFLHAS---EIIHRDVKTTNILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYV 512
           A+A+LH+S    I HRD+K+TNILLD +FN KVADFGLSRL  T+++HISTAPQGTPGY+
Sbjct: 484 AIAYLHSSMNPPIYHRDIKSTNILLDYDFNSKVADFGLSRLGMTESSHISTAPQGTPGYL 543

Query: 513 DPEYHECYQLTNKSDVFSFGVVLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNF 572
           DP+YH+C+ L++KSDV+SFGVVL E+I+    VD TR   EINL+ +A++KI +  +   
Sbjct: 544 DPQYHQCFHLSDKSDVYSFGVVLAEIITGLKVVDFTRPHTEINLAALAVDKIGSGCIDEI 603

Query: 573 VDPSLGFETDQRVRDVIWAVAELAFRCLQSVRDTRPSMLEALETLKNI 584
           +DP L  + D      I  VAELAFRCL    D RP+M E  + L+ I
Sbjct: 604 IDPILDLDLDAWTLSSIHTVAELAFRCLAFHSDMRPTMTEVADELEQI 623

BLAST of Lag0024300 vs. ExPASy TrEMBL
Match: A0A6J1C7V1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111008789 OS=Momordica charantia OX=3673 GN=LOC111008789 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.4e-251
Identity = 461/674 (68.40%), Postives = 512/674 (75.96%), Query Frame = 0

Query: 1   MKISLNPIISNFFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPES 60
           MK S +  ISN F  FI  HLS CF+  A+DE KACG SYNCG LVNI  PFWG ER   
Sbjct: 1   MKTSQSTTISNVF--FIAFHLSLCFTVDAIDEFKACGHSYNCGELVNIAYPFWGNERKGF 60

Query: 61  CGWKEFHLKCKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATL 120
           CG +EF LKCKHNRTTTI+INS+EY +LKINQS++RM IARSDLFDN CP+NQ Q TATL
Sbjct: 61  CGRREFELKCKHNRTTTIQINSLEYNILKINQSDHRMTIARSDLFDNLCPKNQTQ-TATL 120

Query: 121 GEHLRRFRRVTNLGAVGKR--------------------------------------NLQ 180
             HL  F   +N   +  R                                      NL 
Sbjct: 121 DHHL--FVYSSNDQNISVRYNCSAQKEIPETYKFSCGSEEEKNGRANYAFEPSAATWNLL 180

Query: 181 MEECTMNIEVTVAMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGA 240
           +EECTMNI+V V MEGL+EG+KN+  LVE AV+ GFDVEYG  +  AC+ C+ +GG CG 
Sbjct: 181 IEECTMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGG 240

Query: 241 NGTNPFYCICGNGVANPYFFCKAAMLPASK---SNAMTGFGSGIGGILLMSLIFFIRLRL 300
           NGT PFYCIC +G  +PYF   A  L  S     N + G G GIGGI+LM  I  I LRL
Sbjct: 241 NGTYPFYCICRSGEIHPYFCGTAHALEESNDKWKNTVIGVGCGIGGIILMGTISLILLRL 300

Query: 301 HKKKHLRASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGF 360
            K KH  ASSF L NN  DPP+KELE GENYM VPLFS+ EL +ATDKFNPA ELGDGGF
Sbjct: 301 QKNKHAHASSFPLGNNYFDPPSKELEKGENYMVVPLFSYGELEEATDKFNPAKELGDGGF 360

Query: 361 GTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELL 420
           GTVYYGKLRDGREVAVKRLFEN+YRKV+HFMNEV+ILT L H HLVTLYG TSRRSRELL
Sbjct: 361 GTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRELL 420

Query: 421 LVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNIL 480
           LVYEFI NGT+ADHLHGNRA+  ELPW TRLKIAIETASALA+LHAS+ IHRDVKTTNIL
Sbjct: 421 LVYEFIPNGTLADHLHGNRAKSGELPWITRLKIAIETASALAYLHASKTIHRDVKTTNIL 480

Query: 481 LDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLV 540
           LD N +VKVAD GLS L PTQATH+STAPQGTPGY+DPEY ECYQLTNKSDVFSFGVVLV
Sbjct: 481 LDKNLSVKVADLGLSCLSPTQATHVSTAPQGTPGYIDPEYQECYQLTNKSDVFSFGVVLV 540

Query: 541 ELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELA 600
           ELISSKPAVD+TRHRHEINL TMAINKIQNN+LHNFVDPSL FETDQ VRD+I+AVA LA
Sbjct: 541 ELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAVAGLA 600

Query: 601 FRCLQSVRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVI 634
           F+CLQSV+D RPSMLEALE LKNIESRSCG+ K EE+ I  +D+  LK G VPQSP+SV 
Sbjct: 601 FQCLQSVKDMRPSMLEALEILKNIESRSCGRRKTEELDILSEDE--LKVGSVPQSPDSVT 660

BLAST of Lag0024300 vs. ExPASy TrEMBL
Match: A0A6J1E6Z3 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Cucurbita moschata OX=3662 GN=LOC111431355 PE=4 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 9.3e-249
Identity = 454/668 (67.96%), Postives = 505/668 (75.60%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCK 71
           FF  F T HLSPCFS++ALD  K CGV+YNC  LVNIT PFWG ER  SCG KEF L C 
Sbjct: 39  FFFFFFTFHLSPCFSDNALDAFKDCGVNYNCEELVNITYPFWGNERQSSCGRKEFKLNCN 98

Query: 72  HNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATLGEHLRRF-RRV 131
           +NRTTT  INS+EY VL+INQ NN MRIARSDLFDNYCP+NQIQ  A++  H   +  R 
Sbjct: 99  NNRTTTTYINSLEYNVLEINQLNNTMRIARSDLFDNYCPRNQIQ-VASMDHHPFVYSARN 158

Query: 132 TNLGA-------------------------VGKRNLQME----------ECTMNIEVTVA 191
            N+                            G+ N   E          EC M IEV V 
Sbjct: 159 QNISVGYNCSTDNEFPISEYNFSCGGEWERRGRVNYAFEPSAAVYSKLVECEMKIEVMVT 218

Query: 192 MEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNG 251
           ++GL+EGVKN+ SLVETAVK GF+VEY  W+K ACD C  SGG CG NGT PFYCIC NG
Sbjct: 219 IKGLKEGVKNRRSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNG 278

Query: 252 VANPYFFCKAAMLPA----------SKSNAMTGFGSGIGGILLMSLIFFIRLRLHKKKHL 311
           VANPY  C A   P           +    + G GSGIGGI++MS+IFFIRLR+HKKKH 
Sbjct: 279 VANPY-ACDATAAPPRPPHPENPNDTWKKVVIGAGSGIGGIVMMSIIFFIRLRMHKKKH- 338

Query: 312 RASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGTVYYG 371
                  +N SCD P KELE  E+YM + LFS+HELV+ATDKFNPANELGDGGFGTVYYG
Sbjct: 339 -------QNYSCDRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDGGFGTVYYG 398

Query: 372 KLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFI 431
           KLRDGREVAVKRLFEN+YRKV+ FMNEVE+LTRLRHPHLVTLYG TSRRSRELLLVYEFI
Sbjct: 399 KLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRELLLVYEFI 458

Query: 432 SNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFN 491
            NGTVADHLHGNRAR  ELPW TRL +AIETASAL+FLHASE IHRDVKTTNILLDNNF 
Sbjct: 459 PNGTVADHLHGNRARSGELPWLTRLNVAIETASALSFLHASETIHRDVKTTNILLDNNFG 518

Query: 492 VKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSK 551
           VKVADFGL RLFPTQA+H+STAPQGTPGY+DPEY+E YQLTNKSDVFSFGVVLVELISSK
Sbjct: 519 VKVADFGLCRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVVLVELISSK 578

Query: 552 PAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQS 611
           PAVDITRHRHEINLSTMAINKIQN+ELH+FVDPSLGF+TDQR+R++I  VAELAF+CLQS
Sbjct: 579 PAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAELAFQCLQS 638

Query: 612 VRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISK 634
            RDTRPSM+EALE L+NIE+RSCG GKA                LVP+SP+SV V WISK
Sbjct: 639 ARDTRPSMMEALEILRNIENRSCGTGKAP---------------LVPESPDSVTVLWISK 681

BLAST of Lag0024300 vs. ExPASy TrEMBL
Match: A0A6J1KES1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495165 PE=4 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 1.2e-248
Identity = 454/669 (67.86%), Postives = 504/669 (75.34%), Query Frame = 0

Query: 10  SNFFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLK 69
           S FF    T HLS CFS++ALD  K CGVSYNCG LVNIT PFWG E+  SCG KEF L 
Sbjct: 3   SFFFFFLFTFHLSTCFSDNALDAFKDCGVSYNCGELVNITYPFWGNEKQSSCGRKEFKLN 62

Query: 70  CKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQ-------------- 129
           CK NRTTT  INS+EY VL+INQSNN MRIARSDLFDNYCP+NQIQ              
Sbjct: 63  CKSNRTTTTYINSLEYNVLEINQSNNTMRIARSDLFDNYCPRNQIQVASIDHHPFAYSSR 122

Query: 130 ---------------------RTATLGEHLRRFRRVTNLGAVGKRNLQMEECTMNIEVTV 189
                                + +  GE  RR R             ++ EC M IEV V
Sbjct: 123 NQNISVGYNCSPDNEFLISKYKFSCGGEWQRRGRVNYAFEPSAAVYSKLAECEMKIEVMV 182

Query: 190 AMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGN 249
            ++ L+EG+KN+ SLVETAVK GF+VEY  W+K ACD C  SGG CG NGT PFYCIC N
Sbjct: 183 TIKELKEGLKNRTSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRN 242

Query: 250 GVANPYFFCKAAMLPA----------SKSNAMTGFGSGIGGILLMSLIFFIRLRLHKKKH 309
            VANPY  C A   P           +    + G GSGIGGI++MS+IFFIRLR+HKKKH
Sbjct: 243 EVANPY-ACDAIAAPPRPPHPENPNDTWKKVVIGAGSGIGGIVMMSIIFFIRLRMHKKKH 302

Query: 310 LRASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGTVYY 369
                   +N SCD P KELE  E+YM + LFS+HELV+ATDKFNPANELGDGGFGTVYY
Sbjct: 303 --------QNYSCDRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDGGFGTVYY 362

Query: 370 GKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEF 429
           GKLRDGREVAVKRLFEN+YRKV+ FMNEVE+LTRLRHPHLVTLYG TSRRSRELLLVY+F
Sbjct: 363 GKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRELLLVYKF 422

Query: 430 ISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNF 489
           I NGTVADHLHGNRAR  ELPW TRLK+AIETASALAFLHASE IHRDVKTTNILLDNNF
Sbjct: 423 IPNGTVADHLHGNRARSGELPWLTRLKVAIETASALAFLHASETIHRDVKTTNILLDNNF 482

Query: 490 NVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISS 549
            VKVADFGLSRLFPTQA+H+STAPQGTPGY+DPEY+E YQLTNKSDVFSFGVVLVELISS
Sbjct: 483 GVKVADFGLSRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVVLVELISS 542

Query: 550 KPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQ 609
           KPAVDITRHRHEINLSTMAINKIQN+ELH+FVDPSLGF+TDQR+R++I  VAELAF+CLQ
Sbjct: 543 KPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAELAFQCLQ 602

Query: 610 SVRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWIS 634
           SVRDTRPSM+EALE L+NIE+RSCG GKA                LVP+SP+SV V WIS
Sbjct: 603 SVRDTRPSMMEALEILRNIENRSCGTGKAP---------------LVPESPDSVTVLWIS 647

BLAST of Lag0024300 vs. ExPASy TrEMBL
Match: A0A6J1KJE6 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495165 PE=4 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.7e-248
Identity = 454/672 (67.56%), Postives = 504/672 (75.00%), Query Frame = 0

Query: 10  SNFFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLK 69
           S FF    T HLS CFS++ALD  K CGVSYNCG LVNIT PFWG E+  SCG KEF L 
Sbjct: 3   SFFFFFLFTFHLSTCFSDNALDAFKDCGVSYNCGELVNITYPFWGNEKQSSCGRKEFKLN 62

Query: 70  CKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQ-------------- 129
           CK NRTTT  INS+EY VL+INQSNN MRIARSDLFDNYCP+NQIQ              
Sbjct: 63  CKSNRTTTTYINSLEYNVLEINQSNNTMRIARSDLFDNYCPRNQIQVASIDHHPFAYSSR 122

Query: 130 ---------------------RTATLGEHLRRFRRVTNLGAVGKRNLQMEECTMNIEVTV 189
                                + +  GE  RR R             ++ EC M IEV V
Sbjct: 123 NQNISVGYNCSPDNEFLISKYKFSCGGEWQRRGRVNYAFEPSAAVYSKLAECEMKIEVMV 182

Query: 190 AMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGN 249
            ++ L+EG+KN+ SLVETAVK GF+VEY  W+K ACD C  SGG CG NGT PFYCIC N
Sbjct: 183 TIKELKEGLKNRTSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRN 242

Query: 250 GVANPYFFCKAAMLPA-------------SKSNAMTGFGSGIGGILLMSLIFFIRLRLHK 309
            VANPY  C A   P              +    + G GSGIGGI++MS+IFFIRLR+HK
Sbjct: 243 EVANPY-ACDAIAAPPRPPHPSHPENPNDTWKKVVIGAGSGIGGIVMMSIIFFIRLRMHK 302

Query: 310 KKHLRASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGT 369
           KKH        +N SCD P KELE  E+YM + LFS+HELV+ATDKFNPANELGDGGFGT
Sbjct: 303 KKH--------QNYSCDRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDGGFGT 362

Query: 370 VYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLV 429
           VYYGKLRDGREVAVKRLFEN+YRKV+ FMNEVE+LTRLRHPHLVTLYG TSRRSRELLLV
Sbjct: 363 VYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRELLLV 422

Query: 430 YEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLD 489
           Y+FI NGTVADHLHGNRAR  ELPW TRLK+AIETASALAFLHASE IHRDVKTTNILLD
Sbjct: 423 YKFIPNGTVADHLHGNRARSGELPWLTRLKVAIETASALAFLHASETIHRDVKTTNILLD 482

Query: 490 NNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVEL 549
           NNF VKVADFGLSRLFPTQA+H+STAPQGTPGY+DPEY+E YQLTNKSDVFSFGVVLVEL
Sbjct: 483 NNFGVKVADFGLSRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVVLVEL 542

Query: 550 ISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFR 609
           ISSKPAVDITRHRHEINLSTMAINKIQN+ELH+FVDPSLGF+TDQR+R++I  VAELAF+
Sbjct: 543 ISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAELAFQ 602

Query: 610 CLQSVRDTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVP 634
           CLQSVRDTRPSM+EALE L+NIE+RSCG GKA                LVP+SP+SV V 
Sbjct: 603 CLQSVRDTRPSMMEALEILRNIENRSCGTGKAP---------------LVPESPDSVTVL 650

BLAST of Lag0024300 vs. ExPASy TrEMBL
Match: A0A1S4DTL2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499532 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 5.5e-241
Identity = 443/665 (66.62%), Postives = 508/665 (76.39%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCK 71
           FF + ++ HL  CF++ A +E KACGV YNCG LVNI+ PFWG ER   CG +EF L C+
Sbjct: 11  FFFAILSFHLRLCFADDAHEEFKACGVYYNCGELVNISYPFWGNERQSFCGRREFSLTCE 70

Query: 72  HNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATLGEHLRRFRR-- 131
            N+TTTI+I+S +Y V+ I+QS++ M IARS+LFD+YCP N+I   ATL   L ++    
Sbjct: 71  DNKTTTIQIHSRKYIVVNISQSDHIMTIARSELFDDYCPNNEI-GVATLDFSLFKYSSND 130

Query: 132 ---------------VTNL----GAVGKRN------LQMEE----------CTMNIEVTV 191
                            NL    G+ G+RN      L   E          C + IEVT+
Sbjct: 131 LNLSVWYDCPMLPGIPPNLEFECGSEGERNGRANYALAENEAMNWSSYNGGCRIKIEVTI 190

Query: 192 AMEGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGN 251
             +  REG  N+  +VE  +K GF+VEYG ++  AC+GCKE GG CG N T  F CIC +
Sbjct: 191 TSKVWREGNTNRTIVVERGMKEGFEVEYGDFYTIACEGCKEFGGACGRNATKEFLCICKS 250

Query: 252 GVANPYFFCKAAMLPASKSNA----MTGFGSGIGGILLMSLIFFIRLRLHKKKHLRA--- 311
           G  +PY     +  P  +       + G  SGIGG+LLM +  FI   LHKKK  R+   
Sbjct: 251 GDIHPYVCKPPSTTPPEEKKGWMKPVIGVCSGIGGVLLMGVASFIWFCLHKKKLARSYTP 310

Query: 312 SSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGDGGFGTVYYGKL 371
           SSFLLRNNS +P TKELE GEN MG+PLFS+ EL +ATDKFNPA ELGDGGFGTVYYGKL
Sbjct: 311 SSFLLRNNSSEPSTKELEKGENDMGLPLFSYEELEKATDKFNPAKELGDGGFGTVYYGKL 370

Query: 372 RDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFISN 431
            DGREVAVKRLFEN+YR+V+HFMNEVEILTRLRHPHLVTLYG TSR  RELLLVYEFI N
Sbjct: 371 SDGREVAVKRLFENNYRRVEHFMNEVEILTRLRHPHLVTLYGCTSRLCRELLLVYEFIPN 430

Query: 432 GTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFNVK 491
           GTVADHLHGNRA+P ELPWHTRLKIAI+TASALAFLHASE IHRDVKTTNILLDNN+NVK
Sbjct: 431 GTVADHLHGNRAKPGELPWHTRLKIAIDTASALAFLHASETIHRDVKTTNILLDNNYNVK 490

Query: 492 VADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPA 551
           VADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPA
Sbjct: 491 VADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPA 550

Query: 552 VDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQSVR 611
           VDITRHRHEINL TMAINKI+N+ELH+FVD SLGFETD+ VRD+I AVAELAFRCLQSV+
Sbjct: 551 VDITRHRHEINLWTMAINKIRNDELHDFVDTSLGFETDETVRDMICAVAELAFRCLQSVK 610

Query: 612 DTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISKSS 633
           DTRPSMLEALE LK+IESRS GKGK E+IGIS +DD LLK GLVP+SP+SV+VPW+SKSS
Sbjct: 611 DTRPSMLEALEILKDIESRSSGKGKEEDIGISHEDDVLLKDGLVPESPDSVVVPWMSKSS 670

BLAST of Lag0024300 vs. TAIR 10
Match: AT1G18390.1 (Protein kinase superfamily protein )

HSP 1 Score: 512.3 bits (1318), Expect = 5.5e-145
Identity = 300/658 (45.59%), Postives = 398/658 (60.49%), Query Frame = 0

Query: 12  FFISFITL---HLSPCFS-----ESALDESKACGVSYNCGALVNITDPFWGK-ERPESCG 71
           FFISF+      L  CFS     E     +  CG  +      + T PFWG   +P+ CG
Sbjct: 6   FFISFVVFSVADLPSCFSADQQYEECRSRNLTCGSGHR--VFESTTYPFWGGFNKPKFCG 65

Query: 72  WKEFHLKCKHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFD-------NYCPQNQIQ 131
              F L C+ ++  T+ I ++   V+  N  ++++ +A   L D       N+  +NQ  
Sbjct: 66  HSSFKLSCEGDQNLTLAIGNITLRVVSANLEDHKISVADDSLLDGGCLNIWNFNGKNQF- 125

Query: 132 RTATLGEHLRRFRRVTNLGAVGKRNLQMEE-------------------CTMNIEVTVAM 191
              TL  +        N   V    +  EE                   C    E+ +  
Sbjct: 126 ---TLDSNTETIDVFVNCSGVAPLQISCEESYEDPVTYHVLRSSDSDEGCMKYAEIPMLR 185

Query: 192 EGLREGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCG-ANGTNPFYCICGNG 251
               E  +++++ VE A++ GFD+ Y    K AC  C +SGG CG A  +  F C+C + 
Sbjct: 186 SAKDELQRSELTFVE-ALRKGFDLRYIMEDK-ACRRCIDSGGICGSALDSESFRCLCADR 245

Query: 252 VANPYFFCKAAMLPASKS-----NAMTGFGSGIGGILLMSLIFFIRLRLHKKKHLRASSF 311
             N             K        + G  + + G++  S+ +++  R   K +  +S+ 
Sbjct: 246 PHNSSCDDNTNQGKNDKRRRVIVKVLIGASAAVVGLIAASIFWYVYHRRKTKSYRNSSAL 305

Query: 312 LLRNNSCDPPTK--ELENGENYM-GVPLFSFHELVQATDKFNPANELGDGGFGTVYYGKL 371
           L RN S DP  K  ++E  E  + GV +FS+ EL +AT+ F+P+ ELGDGGFGTVYYGKL
Sbjct: 306 LPRNISSDPSAKSFDIEKAEELLVGVHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKL 365

Query: 372 RDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFISN 431
           +DGR VAVKRL++N++++ + F NEVEILT LRHP+LV L+G +S++SR+LLLVYE+++N
Sbjct: 366 KDGRSVAVKRLYDNNFKRAEQFRNEVEILTGLRHPNLVALFGCSSKQSRDLLLVYEYVAN 425

Query: 432 GTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFNVK 491
           GT+ADHLHG +A PS LPW  RLKIA+ETASAL +LHAS+IIHRDVK+ NILLD NFNVK
Sbjct: 426 GTLADHLHGPQANPSSLPWSIRLKIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVK 485

Query: 492 VADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPA 551
           VADFGLSRLFP   TH+STAPQGTPGYVDP+YH CYQL+NKSDV+SF VVL+ELISS PA
Sbjct: 486 VADFGLSRLFPMDKTHVSTAPQGTPGYVDPDYHLCYQLSNKSDVYSFAVVLMELISSLPA 545

Query: 552 VDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQSVR 611
           VDITR R EINLS MA+ KIQN+EL + VDPSLGF+TD RVR  + AVAELAF+CLQS +
Sbjct: 546 VDITRPRQEINLSNMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDK 605

Query: 612 DTRPSMLEALETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISK 626
           D RP M    +TL  I++   G     E+ +    D    G LV QSP+SVIV W SK
Sbjct: 606 DLRPCMSHVQDTLTRIQNNGFG----SEMDVV---DVNKSGPLVAQSPDSVIVKWDSK 648

BLAST of Lag0024300 vs. TAIR 10
Match: AT5G38210.1 (Protein kinase family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.4e-135
Identity = 291/659 (44.16%), Postives = 392/659 (59.48%), Query Frame = 0

Query: 12  FFISFITLHLSPCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKC- 71
           FF+S+I  H  PC    A  + + C   + CG L     PFWG  RP+ CG     L C 
Sbjct: 20  FFLSYI--HFLPC----AQSQREPCDTLFRCGDL-TAGFPFWGVARPQPCGHPSLGLHCQ 79

Query: 72  KHNRTTTIEINSMEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTAT--LGEHLRRFR 131
           K   +T++ I+S+ Y VL++N + + +++ R D    +C  +    T T  L E L  ++
Sbjct: 80  KQTNSTSLIISSLMYRVLEVNTTTSTLKLVRQDFSGPFCSASFSGATLTPELFELLPDYK 139

Query: 132 RVT--------------------NLGAVGKRNLQMEECTMNIEVTVAMEGLREGVKNKMS 191
            ++                     +G++ + +L    C     +TV +    E     ++
Sbjct: 140 TLSAYYLCNPSLHYPAKFICPNKGVGSIHQDDLYHNHCGGIFNITVPIGYAPEEGALNVT 199

Query: 192 LVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNGVANPYFFCKAAML 251
            +E+ +K GF+V+     +  C  CK +GG C  +   P   +C    ++    C   M 
Sbjct: 200 NLESVLKKGFEVKL-SIDERPCQECKTNGGICAYHVATP---VCCKTNSSSEVNCTPMMP 259

Query: 252 PASKSNA--------MTGFGSG-IGGILLMSLIFFIRLRLHKKKHLR------------A 311
             S ++A          GF SG +G  L+   +  I +R  KK   +            +
Sbjct: 260 SGSSAHAGLSKKGKIGIGFASGFLGATLIGGCLLCIFIRRRKKLATQYTNKGLSTTTPYS 319

Query: 312 SSFLL-----------RNNSCDPPTKELENGENYMGVPLFSFHELVQATDKFNPANELGD 371
           S++ +            N+S  P    L NG  Y G+ +FS+ EL +AT+ F  + ELGD
Sbjct: 320 SNYTMSNTPTSTTISGSNHSLVPSISNLGNGSVYSGIQVFSYEELEEATENF--SKELGD 379

Query: 372 GGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSR 431
           GGFGTVYYG L+DGR VAVKRLFE S ++V+ F NE++IL  L+HP+LV LYG T+R SR
Sbjct: 380 GGFGTVYYGTLKDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSR 439

Query: 432 ELLLVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTT 491
           ELLLVYE+ISNGT+A+HLHGN+A+   + W  RL+IAIETASAL++LHAS IIHRDVKTT
Sbjct: 440 ELLLVYEYISNGTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTT 499

Query: 492 NILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGV 551
           NILLD+N+ VKVADFGLSRLFP   THISTAPQGTPGYVDPEY++CY+L  KSDV+SFGV
Sbjct: 500 NILLDSNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGV 559

Query: 552 VLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVA 611
           VL ELISSK AVDITRHRH+INL+ MAI+KIQN+ +H   D SLGF  D  V+ ++ +VA
Sbjct: 560 VLSELISSKEAVDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVA 619

Query: 612 ELAFRCLQSVRDTRPSMLEALETLKNIESR--SCGKGKAEEIGISGDDD-GLLKGGLVP 613
           ELAFRCLQ  RD RPSM E +E L+ I+    S  K    EI ++G DD GLLK G+ P
Sbjct: 620 ELAFRCLQQERDVRPSMDEIVEVLRVIQKDGISDSKDVVVEIDVNGGDDVGLLKHGVPP 665

BLAST of Lag0024300 vs. TAIR 10
Match: AT1G18390.2 (Protein kinase superfamily protein )

HSP 1 Score: 478.8 bits (1231), Expect = 6.8e-135
Identity = 256/468 (54.70%), Postives = 331/468 (70.73%), Query Frame = 0

Query: 167 MSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNPFYCICGNGVANPYFFC--- 226
           M+ VE  +K GF +    W   +C  C  SGG+CG +    F C+C +G    +  C   
Sbjct: 200 MTYVE-ILKRGFVL---NWTANSCFRCITSGGRCGTD-QQEFVCLCPDG-PKLHDTCTNG 259

Query: 227 ---KAAMLPASKSNAMTGFGSGIGGILLMSLIFFIRLRLHKKKHLRASSFLLRNNSCDPP 286
              K   +    + +++G  + + G++  S+ +++  R   K +  +S+ L RN S DP 
Sbjct: 260 KNDKRRRVIVKITKSISGASAAVVGLIAASIFWYVYHRRKTKSYRNSSALLPRNISSDPS 319

Query: 287 TK--ELENGENYM-GVPLFSFHELVQATDKFNPANELGDGGFGTVYYGKLRDGREVAVKR 346
            K  ++E  E  + GV +FS+ EL +AT+ F+P+ ELGDGGFGTVYYGKL+DGR VAVKR
Sbjct: 320 AKSFDIEKAEELLVGVHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKR 379

Query: 347 LFENSYRKVQHFMNEVEILTRLRHPHLVTLYGSTSRRSRELLLVYEFISNGTVADHLHGN 406
           L++N++++ + F NEVEILT LRHP+LV L+G +S++SR+LLLVYE+++NGT+ADHLHG 
Sbjct: 380 LYDNNFKRAEQFRNEVEILTGLRHPNLVALFGCSSKQSRDLLLVYEYVANGTLADHLHGP 439

Query: 407 RARPSELPWHTRLKIAIETASALAFLHASEIIHRDVKTTNILLDNNFNVKVADFGLSRLF 466
           +A PS LPW  RLKIA+ETASAL +LHAS+IIHRDVK+ NILLD NFNVKVADFGLSRLF
Sbjct: 440 QANPSSLPWSIRLKIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLSRLF 499

Query: 467 PTQATHISTAPQGTPGYVDPEYHECYQLTNKSDVFSFGVVLVELISSKPAVDITRHRHEI 526
           P   TH+STAPQGTPGYVDP+YH CYQL+NKSDV+SF VVL+ELISS PAVDITR R EI
Sbjct: 500 PMDKTHVSTAPQGTPGYVDPDYHLCYQLSNKSDVYSFAVVLMELISSLPAVDITRPRQEI 559

Query: 527 NLSTMAINKIQNNELHNFVDPSLGFETDQRVRDVIWAVAELAFRCLQSVRDTRPSMLEAL 586
           NLS MA+ KIQN+EL + VDPSLGF+TD RVR  + AVAELAF+CLQS +D RP M    
Sbjct: 560 NLSNMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMSHVQ 619

Query: 587 ETLKNIESRSCGKGKAEEIGISGDDDGLLKGGLVPQSPNSVIVPWISK 626
           +TL  I++   G     E+ +    D    G LV QSP+SVIV W SK
Sbjct: 620 DTLTRIQNNGFG----SEMDVV---DVNKSGPLVAQSPDSVIVKWDSK 654

BLAST of Lag0024300 vs. TAIR 10
Match: AT1G66880.1 (Protein kinase superfamily protein )

HSP 1 Score: 463.4 bits (1191), Expect = 3.0e-130
Identity = 296/687 (43.09%), Postives = 386/687 (56.19%), Query Frame = 0

Query: 23   PCFSESALDESKACGVSYNCGALVNITDPFWGKERPESCGWKEFHLKCKHNRTTTIEINS 82
            P    + L+  + C  +++CG    +  PFW   R E CG  +F L     R   + I+S
Sbjct: 618  PLPDATELELHRRCNGTFSCGDQRELFYPFWTSGR-EDCGHPDFKLDDCSGRFAELSISS 677

Query: 83   MEYYVLKINQSNNRMRIARSDLFDNYCPQNQIQRTATLGEHLRRF--------------- 142
            +++ +L     +N +R+ RS+   + CPQ+ I   A   E +  F               
Sbjct: 678  VKFRILASVYGSNIIRLGRSEYIGDLCPQDPI--NAPFSESVLPFAPNTELLTIFYNCSR 737

Query: 143  ---RRVTNLG--AVG-----------KRNLQM--------------EECTMNIEVTVAME 202
               ++VTN G  A G            RNL                + C  N+ +  +  
Sbjct: 738  DFPQQVTNFGDFACGDDSDDDRSYYVTRNLSFPPLSEINDLLYDFSQSCDRNVSIPASGS 797

Query: 203  GLR--EGVKNKMSLVETAVKHGFDVEYGKWFKEACDGCKESGGKCGANGTNP-FYCIC-- 262
             L   +   +  +L + A+++GF++E      + C  C +S G CG + T+  F C    
Sbjct: 798  TLNILQSTPSNDNL-KKALEYGFELE----LNQDCRTCIDSKGACGYSQTSSRFVCYSIE 857

Query: 263  ---GNGVANPYFFCKAAMLPASKSNAMTGFGSGIGGILLMSLIFFIRLR------LHKKK 322
                    NP      ++   +K+       SG+  +LL  L   IR R       +  K
Sbjct: 858  EPQTPTPPNPTRNKDTSLSIGAKAGIAVASVSGLAILLLAGLFLCIRRRRKTQDAQYTSK 917

Query: 323  HLRASSFLLR---------------NNSCDPPTKELENGENYMGVPLFSFHELVQATDKF 382
             L  +S+  R               N+S  P    L N  +Y GV +FS+ EL +AT+ F
Sbjct: 918  SLPITSYSSRDTSRNPTSTTISSSSNHSLLPSISNLANRSDYCGVQVFSYEELEEATENF 977

Query: 383  NPANELGDGGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLY 442
              + ELGDGGFGTVYYG L+DGR VAVKRL+E S ++V+ F NE+EIL  L+HP+LV LY
Sbjct: 978  --SRELGDGGFGTVYYGVLKDGRAVAVKRLYERSLKRVEQFKNEIEILKSLKHPNLVILY 1037

Query: 443  GSTSRRSRELLLVYEFISNGTVADHLHGNRARPSELPWHTRLKIAIETASALAFLHASEI 502
            G TSR SRELLLVYE+ISNGT+A+HLHGNRA    L W TRL IAIETASAL+FLH   I
Sbjct: 1038 GCTSRHSRELLLVYEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSFLHIKGI 1097

Query: 503  IHRDVKTTNILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTNK 562
            IHRD+KTTNILLD+N+ VKVADFGLSRLFP   THISTAPQGTPGYVDPEY++CYQL  K
Sbjct: 1098 IHRDIKTTNILLDDNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYQLNEK 1157

Query: 563  SDVFSFGVVLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQRV 622
            SDV+SFGVVL ELISSK AVDITRHRH+INL+ MA++KIQNN LH  VD SLG++ D  V
Sbjct: 1158 SDVYSFGVVLTELISSKEAVDITRHRHDINLANMAVSKIQNNALHELVDSSLGYDNDPEV 1217

Query: 623  RDVIWAVAELAFRCLQSVRDTRPSMLEALETLKNI---ESRSCGKGKAEEIGI---SGDD 629
            R  + AVAELAFRCLQ  RD RP+M E +E L+ I   E +       + + I    GDD
Sbjct: 1218 RRKMMAVAELAFRCLQQERDVRPAMDEIVEILRGIKDDEKKRVLVKSPDVVDIECGGGDD 1277

BLAST of Lag0024300 vs. TAIR 10
Match: AT1G25390.1 (Protein kinase superfamily protein )

HSP 1 Score: 441.8 bits (1135), Expect = 9.2e-124
Identity = 249/454 (54.85%), Postives = 307/454 (67.62%), Query Frame = 0

Query: 190 CDGCKESGGKCGANGTNPFYCICGNGVANPYFFCKAAMLPASKSNAMTGFGSGIGG-ILL 249
           C  C  +GG+C     N + C+  N   N Y             +A    G GIGG ++L
Sbjct: 190 CFRCHNNGGEC-TKVKNNYRCVGANTEPNNY-------------HAEMRLGLGIGGSVIL 249

Query: 250 MSLIFFIRLRLHKKKHLRASSFLLRNNSCDPPTKELENGENYMGVPLFSFHELVQATDKF 309
           + ++  +   +H+    +  S L R+NS      ++E  + +  +P+FS+ EL  ATD F
Sbjct: 250 IIILVALFAVIHRNYRRKDGSELSRDNS----KSDVEFSQVFFKIPIFSYKELQAATDNF 309

Query: 310 NPANELGDGGFGTVYYGKLRDGREVAVKRLFENSYRKVQHFMNEVEILTRLRHPHLVTLY 369
           +    LGDGGFGTVYYGK+RDGREVAVKRL+E++YR+++ FMNE+EILTRL H +LV+LY
Sbjct: 310 SKDRLLGDGGFGTVYYGKVRDGREVAVKRLYEHNYRRLEQFMNEIEILTRLHHKNLVSLY 369

Query: 370 GSTSRRSRELLLVYEFISNGTVADHLHG-NRARPSELPWHTRLKIAIETASALAFLHASE 429
           G TSRRSRELLLVYEFI NGTVADHL+G N      L W  RL IAIETASALA+LHAS+
Sbjct: 370 GCTSRRSRELLLVYEFIPNGTVADHLYGENTPHQGFLTWSMRLSIAIETASALAYLHASD 429

Query: 430 IIHRDVKTTNILLDNNFNVKVADFGLSRLFPTQATHISTAPQGTPGYVDPEYHECYQLTN 489
           IIHRDVKTTNILLD NF VKVADFGLSRL P+  TH+STAPQGTPGYVDPEYH CY LT+
Sbjct: 430 IIHRDVKTTNILLDRNFGVKVADFGLSRLLPSDVTHVSTAPQGTPGYVDPEYHRCYHLTD 489

Query: 490 KSDVFSFGVVLVELISSKPAVDITRHRHEINLSTMAINKIQNNELHNFVDPSLGFETDQR 549
           KSDV+SFGVVLVELISSKPAVDI+R + EINLS++AINKIQN+  H  +D +LG+ T++ 
Sbjct: 490 KSDVYSFGVVLVELISSKPAVDISRCKSEINLSSLAINKIQNHATHELIDQNLGYATNEG 549

Query: 550 VRDVIWAVAELAFRCLQSVRDTRPSMLEALETLKNI--ESRSCGKGKAEEIGI----SGD 609
           VR +   VAELAF+CLQ     RP+M + +  LK I  E + C      E  I    S  
Sbjct: 550 VRKMTTMVAELAFQCLQQDNTMRPTMEQVVHELKGIQNEEQKCPTYDYREETIIPHPSPP 609

Query: 610 DDG---LLKGGLVPQSPNSVIVPWISKSSTPNGS 633
           D G   LLK    P+SP SV   W SKS+TPN S
Sbjct: 610 DWGEAALLKNMKFPRSPVSVTDQWTSKSTTPNTS 625