Lag0024274 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024274
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionS-protein homolog
Locationchr10: 1779667 .. 1780089 (-)
RNA-Seq ExpressionLag0024274
SyntenyLag0024274
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCCTCTAAGCATCTTTTGTTTCATCATATTGCTTGCTTTGGAATCAAACATGTTCAAGTCATGTTTTGCAGCACGTCCATTGTCCCCATGGGAAGTGGCCATTTTGAATAATATGAAGGATTCAACTCTTATTGTTCATTGCAAGTCGAAAGACGACAATTTGGGCGAGCACGTGGTCAACGTTGGAGGCAAATACTATTGGATGTTCAGTGAAAATCTTTGGCAAACGACATTGTATTGGTGCAATTTTAGCAGCAAATATGGACAAACTTCTGGTGAAGTTTTCTGGCCAGAAAAGGGGAGTTGGCTTAGTGATCGATGTGATCGTAATACCTGCATTTGGGCTGCTCATGAGCGTGGGATTTCCTTGCGTCTTGGTCCCCTCAATTCTTTTCAATTAATCTATCCTTGGAAATGA

mRNA sequence

ATGAAGCCTCTAAGCATCTTTTGTTTCATCATATTGCTTGCTTTGGAATCAAACATGTTCAAGTCATGTTTTGCAGCACGTCCATTGTCCCCATGGGAAGTGGCCATTTTGAATAATATGAAGGATTCAACTCTTATTGTTCATTGCAAGTCGAAAGACGACAATTTGGGCGAGCACGTGGTCAACGTTGGAGGCAAATACTATTGGATGTTCAGTGAAAATCTTTGGCAAACGACATTGTATTGGTGCAATTTTAGCAGCAAATATGGACAAACTTCTGGTGAAGTTTTCTGGCCAGAAAAGGGGAGTTGGCTTAGTGATCGATGTGATCGTAATACCTGCATTTGGGCTGCTCATGAGCGTGGGATTTCCTTGCGTCTTGGTCCCCTCAATTCTTTTCAATTAATCTATCCTTGGAAATGA

Coding sequence (CDS)

ATGAAGCCTCTAAGCATCTTTTGTTTCATCATATTGCTTGCTTTGGAATCAAACATGTTCAAGTCATGTTTTGCAGCACGTCCATTGTCCCCATGGGAAGTGGCCATTTTGAATAATATGAAGGATTCAACTCTTATTGTTCATTGCAAGTCGAAAGACGACAATTTGGGCGAGCACGTGGTCAACGTTGGAGGCAAATACTATTGGATGTTCAGTGAAAATCTTTGGCAAACGACATTGTATTGGTGCAATTTTAGCAGCAAATATGGACAAACTTCTGGTGAAGTTTTCTGGCCAGAAAAGGGGAGTTGGCTTAGTGATCGATGTGATCGTAATACCTGCATTTGGGCTGCTCATGAGCGTGGGATTTCCTTGCGTCTTGGTCCCCTCAATTCTTTTCAATTAATCTATCCTTGGAAATGA

Protein sequence

MKPLSIFCFIILLALESNMFKSCFAARPLSPWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFSENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRLGPLNSFQLIYPWK
Homology
BLAST of Lag0024274 vs. NCBI nr
Match: XP_023522257.1 (S-protein homolog 1-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 83/115 (72.17%), Postives = 95/115 (82.61%), Query Frame = 0

Query: 19  MFKSCFA------ARPLSPWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFS 78
           +  SCFA       RPLSPWEVAILN+MKDSTL+ HCKSK+D+LGEHV+  GGKYYWMFS
Sbjct: 2   IINSCFAQSDSKYQRPLSPWEVAILNHMKDSTLLAHCKSKNDDLGEHVIVPGGKYYWMFS 61

Query: 79  ENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRL 128
           EN+WQ+TL+WCNFSSK+GQ SGEVFWP+ G WL DRC  NTCIWAA + GISLRL
Sbjct: 62  ENVWQSTLFWCNFSSKHGQASGEVFWPDNGGWLQDRCASNTCIWAARDDGISLRL 116

BLAST of Lag0024274 vs. NCBI nr
Match: XP_022954425.1 (S-protein homolog 74-like [Cucurbita moschata])

HSP 1 Score: 189.1 bits (479), Expect = 2.6e-44
Identity = 81/128 (63.28%), Postives = 97/128 (75.78%), Query Frame = 0

Query: 19  MFKSCFAAR------PLSPWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFS 78
           +  SCFA          S WEV I+N++KDSTL+VHCKSKDD+LGEHV+  G KY+W F 
Sbjct: 2   IINSCFAQSDSSLIDQFSNWEVDIVNDLKDSTLVVHCKSKDDDLGEHVIKAGDKYFWKFH 61

Query: 79  ENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRLGPLNS 138
           EN+ QTTLYWCNFSSK GQ SG+VFWPEK  WLSDRC+RNTCIW A + GIS+RLG    
Sbjct: 62  ENVLQTTLYWCNFSSKRGQASGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRLGAFGL 121

Query: 139 FQLIYPWK 141
           ++L+YPWK
Sbjct: 122 YELVYPWK 129

BLAST of Lag0024274 vs. NCBI nr
Match: XP_023542206.1 (S-protein homolog 74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 188.7 bits (478), Expect = 3.3e-44
Identity = 76/111 (68.47%), Postives = 91/111 (81.98%), Query Frame = 0

Query: 30  SPWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFSENLWQTTLYWCNFSSKY 89
           S WEV I+N++KDSTL+ HCKSK D+LGEHV+  G KYYW F EN+WQTTLYWCNFSSK 
Sbjct: 51  SNWEVDIVNDLKDSTLVAHCKSKSDDLGEHVIKPGDKYYWKFHENVWQTTLYWCNFSSKR 110

Query: 90  GQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRLGPLNSFQLIYPWK 141
           GQ SG+VFWPEK  WLSDRC+RNTCIW A + GIS+R+G    ++L+YPWK
Sbjct: 111 GQASGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRIGVFGLYELVYPWK 161

BLAST of Lag0024274 vs. NCBI nr
Match: XP_022954429.1 (S-protein homolog 74-like [Cucurbita moschata])

HSP 1 Score: 186.4 bits (472), Expect = 1.7e-43
Identity = 80/128 (62.50%), Postives = 96/128 (75.00%), Query Frame = 0

Query: 19  MFKSCFAAR------PLSPWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFS 78
           +  SCFA          S WEV I+N++KDSTL+ HCKSKDD+LGEHV+  G KY+W F 
Sbjct: 2   IINSCFAQSDSSLIDQFSNWEVDIVNDLKDSTLVAHCKSKDDDLGEHVIKPGDKYFWKFH 61

Query: 79  ENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRLGPLNS 138
           EN+ QTTLYWCNFSSK GQ SG+VFWPEK  WLSDRC+RNTCIW A + GIS+RLG    
Sbjct: 62  ENVLQTTLYWCNFSSKRGQASGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRLGVFGL 121

Query: 139 FQLIYPWK 141
           ++L+YPWK
Sbjct: 122 YELVYPWK 129

BLAST of Lag0024274 vs. NCBI nr
Match: XP_022954427.1 (S-protein homolog 74-like [Cucurbita moschata])

HSP 1 Score: 183.7 bits (465), Expect = 1.1e-42
Identity = 79/128 (61.72%), Postives = 95/128 (74.22%), Query Frame = 0

Query: 19  MFKSCFAAR------PLSPWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFS 78
           +  SCFA          S WEV I+N++KDSTL+ HCKSKDD+LGEHV+  G KY+W F 
Sbjct: 2   IINSCFAQSDSSLIDQFSNWEVDIVNDLKDSTLVAHCKSKDDDLGEHVIKPGDKYFWKFH 61

Query: 79  ENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRLGPLNS 138
           EN+ QTTLYWCNFSSK GQ  G+VFWPEK  WLSDRC+RNTCIW A + GIS+RLG    
Sbjct: 62  ENVLQTTLYWCNFSSKGGQAFGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRLGVFGL 121

Query: 139 FQLIYPWK 141
           ++L+YPWK
Sbjct: 122 YELVYPWK 129

BLAST of Lag0024274 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.4e-15
Identity = 42/139 (30.22%), Postives = 68/139 (48.92%), Query Frame = 0

Query: 8   CFIILLALESNMFKSCFAAR-----PLSPWEVAILNNM-KDSTLIVHCKSKDDNLGEHVV 67
           CF ++L  + ++       R      +S W+V + N +    TL +HCKSK+++LG+  +
Sbjct: 12  CFYLVLTCQDHVLARDTTTRDIVVPKISEWQVTVANGLTTGETLFIHCKSKENDLGDINL 71

Query: 68  NVGGKYYWMFSENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHER 127
               ++ W F EN+  +TL+WC  S   G  + +VFW +    L  RCD   C+W A   
Sbjct: 72  KFLDRFSWNFGENMLHSTLFWCYMSKDDGHMNVKVFWDD--VILFHRCDWKNCVWTAKND 131

Query: 128 GISLRLGPLNSFQLIYPWK 141
           G+ L    +    L   WK
Sbjct: 132 GLYLWNSAIGEDVLSEKWK 148

BLAST of Lag0024274 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-13
Identity = 40/128 (31.25%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 4   LSIFCFIILLALESNMFKSCFAAR-----PLSPWEVAILNNM-KDSTLIVHCKSKDDNLG 63
           L   CF + L  + ++       R      +S W+V ++N +    TL +HCKSK+D+LG
Sbjct: 8   LLAICFSLALTCQDHVLVGGTTTRDIIVPKISEWQVTVVNGLTTGETLFIHCKSKEDDLG 67

Query: 64  EHVVNVGGKYYWMFSENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSDRCDRNTCIWA 123
           E  +    ++ W F EN+  +T +WC  +   G  +  VFW +    L  RC    CIW 
Sbjct: 68  EINLKFRNRFSWNFGENMLHSTFFWCYMNKDNGHMNVNVFWDD--VILFHRCGWKNCIWT 127

Query: 124 AHERGISL 126
           A   G+ L
Sbjct: 128 AKTDGLYL 133

BLAST of Lag0024274 vs. ExPASy Swiss-Prot
Match: F4JLQ5 (S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.0e-12
Identity = 35/113 (30.97%), Postives = 55/113 (48.67%), Query Frame = 0

Query: 28  PLSPWEVAILNNMKDS-TLIVHCKSKDDNLGEHVVNVGGKYYWMFSENLWQTTLYWCNFS 87
           P S   V I N++ +  TL+ HCKSKDD+LG   +  G  + + F    +  TLY+C+FS
Sbjct: 44  PTSKRTVEINNDLGNQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGRTLYFCSFS 103

Query: 88  SKYGQTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGISLRLGPLNSFQLIYPW 140
                 S +++   + S   ++C+ + C+W     G          F L YPW
Sbjct: 104 WPNESHSFDIYKDHRDSGGDNKCESDRCVWKIRRNGPCRFNDETKQFDLCYPW 156

BLAST of Lag0024274 vs. ExPASy Swiss-Prot
Match: F2Q9V4 (S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-11
Identity = 39/93 (41.94%), Postives = 48/93 (51.61%), Query Frame = 0

Query: 31  PWEVAILNNMKDSTLIVHCKSKDDNLGEHVVNVGGKYYWMFSENLWQTTLYWCNFSSKYG 90
           P    ++ N  D  L VHCKS+DD+ G H++  GG Y WMF  N   +TLY+C FS +  
Sbjct: 30  PLTRVVIYNDNDYLLGVHCKSRDDDHGFHILQKGGLYGWMFYVNFMNSTLYFCGFSQE-- 89

Query: 91  QTSGEVFWPEKGSWLSDRCDRNTCIWAAHERGI 124
           Q    VF   K    S RC    C W A E GI
Sbjct: 90  QVKKGVFDIYKAVRDSSRC--RNCTWEAKEDGI 118

BLAST of Lag0024274 vs. ExPASy Swiss-Prot
Match: P0DN93 (S-protein homolog 29 OS=Arabidopsis thaliana OX=3702 GN=SPH29 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-10
Identity = 38/138 (27.54%), Postives = 61/138 (44.20%), Query Frame = 0

Query: 9   FIILLALESNMFKSCFAARPLSPWEVAILNNMK-DSTLIVHCKSKDDNLGEHVVNVGGKY 68
           F++L  +   +  SC    P +   V + NN+   +TL + C+SKDD+LGEH++  G  +
Sbjct: 8   FVVLSIILFYVISSCHGYNPFAKTVVTVTNNISPQTTLTISCRSKDDDLGEHLLLHGQAF 67

Query: 69  YWMFSENLWQTTLYWCNFSSKYGQTSGEVFWPEKGSWLSD-RCDRN-----TCIWAAHER 128
            W F  + ++TTL+ C F            W     W    R DR+     +C W+ +  
Sbjct: 68  LWKFRPSWFRTTLFTCKF-----------LWNNNVKWFDTYRSDRDQGHCYSCNWSINAD 127

Query: 129 GISLRLGPLNSFQLIYPW 140
              +       F   YPW
Sbjct: 128 SACISGNFNKKFDRCYPW 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023522257.11.1e-4472.17S-protein homolog 1-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022954425.12.6e-4463.28S-protein homolog 74-like [Cucurbita moschata][more]
XP_023542206.13.3e-4468.47S-protein homolog 74-like [Cucurbita pepo subsp. pepo][more]
XP_022954429.11.7e-4362.50S-protein homolog 74-like [Cucurbita moschata][more]
XP_022954427.11.1e-4261.72S-protein homolog 74-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q2HQ463.4e-1530.22S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1[more]
F4JLS01.4e-1331.25S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1[more]
F4JLQ56.0e-1230.97S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1[more]
F2Q9V42.3e-1141.94S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2[more]
P0DN931.9e-1027.54S-protein homolog 29 OS=Arabidopsis thaliana OX=3702 GN=SPH29 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010264Plant self-incompatibility S1PFAMPF05938Self-incomp_S1coord: 34..139
e-value: 3.4E-21
score: 75.7
IPR010264Plant self-incompatibility S1PANTHERPTHR31232FAMILY NOT NAMEDcoord: 9..139
NoneNo IPR availablePANTHERPTHR31232:SF93BINDING PROTEIN, PUTATIVE-RELATEDcoord: 9..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024274.1Lag0024274.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process