Lag0023420 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0023420
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionStructure-specific endonuclease subunit SLX1 homolog
Locationchr7: 48109176 .. 48111307 (-)
RNA-Seq ExpressionLag0023420
SyntenyLag0023420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGAGGAAAGAGAAGCCAGAAATCTCTAAAACCATGGCTGTTGCAGAAGAAGAGAATGAAGACGGTGAAGAAGAAGAAAGGGGCAACGAAACGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCCTGCCCTCGTTACAAAGGCCATACCTATATCGGGTTCGAATCTTTTCATCTTCTCTTTACGTCACTCGAACACTTCTCTGTTCTTGTATCCATAGATCGGATCCTTTATTTGCCTTGAAAACCCCCAATTTACTATCTCTGGATGCTCGAATTTGTTTCTCTACTTCCCAATACTGGAAATTTCTTTACGATCTTTGATTCGGTTCTTTGATCCTGTAAATCTTTTGCTTCCCGTATAAATTGATTGAGGAAAAGCATCAAGAAATGTGGGAAATGAGTAAATCAATCTGCACTGGGCTGATTGGATGACTGTTTATATGGAATTTCAGATTTACAGTGAACCCGAAACGTAGAATCCGGCAGCACAATGGCGAAATTAGATGCGGTGCGTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTTTTGTGCATTTATGGCTTCCCCACTAATGTCTCTGCTCTTCAGGTTACATTTCCAGGCTTTGAGCTATACAAGTAGTTGATTGTGCGTCTTTTATATCTTGATTGCCTTCCATTAGCTGAATCACACTTTGGATGATAATATGTGATTGTTAAGAATACTTATTAGGGATTTCAGGCTTCAACATTTTACTAATGTGTTGGTGGTTTCCTTTTTAACCTCTGTCATGATTTATTTTCTTGTAAGTTCCAACAATGCTAGAATTTGATCTTCCAACTATGGATGCAGTTTGAATGGGCCTGGCAACATCCTAATGAGTCATTGGCTGTTAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCCAACAAAGTTAAACTTGCATACACTATGCTCACTCTTCCAGCTTGGTGCAGGTATGAGCAAACATGTATAATTCTATTAATTTTGGCTGTCAAATTGGTTGGAAATGTGAACATAATCCTCCATGTTTGCGAGAAAAGGGAGAGATTTATAGCATGTAAATAAGGACAATATCTCCATTACTATGAGACTGTTTTGGATAACCAAATGTAGTAGGGTCAAGTAGTTGTCCCGTGAGATTAGTTGGGTACGTGCAAGCTGGCTCGAACACTCATGGATATAAAAAAAAACTATGAGACATTTTTGGGTGATACTGGAAGTAGATTCGTTGAAATAAGTCCAAAAACAATATCATACCATTGTAGAAATATGAAAGAAATTTGTTGTCTAAACTCAAGCCACTGTTATTTCCCTTTTTTCTGCCAAACTCCCTGTTCACTTTTTTCTCCTTATCCGTGCAGTTTGAATATCACTGTAAACTACTTCTCAACAAAGTACATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCTGAGCATATGAAGGTCCAAGTTTGCCCCATCAGTGAGCTTCCATGCTATTCTGAAGGAGATCAAGGTGTACTTGGAAATGAAGATGACTGGGAGTATAATAGAGAATATGAAGAAATTTGTAGTTTTCAAGTATATGGATCGATGAAAGAAGTTTCAAATGAAATTCCTCAAAAGTTAATGGATTTCCAGACAGGTACAGATGGAAAACCTAATGAATTGCATGGATGTGATAAAGAACTCGAGGACAATGAACGAGTGCCACCTTCTTCGTGTACTCCATCTGATAACGACGCAGATATGTCTTATGAACGCGATAAAGAACTCAAGAATGATGAACGAGAGCCACCTTCTTGTGCTCCGTCTCATATCATAGCGGGTATGTCTGGCACTGAAATCATCATCGAAGATGGAGAGGAAGACCAATTAGAAGGGAGGAGTGATATGAACTTGCATCAACAACCTGGTAGGAGGAATTTAACATCAGGAATGGCTACTAAAATGATCAAAGACTCAAGATGTAACAATGGGGTTCCTCCCGTTGAGTATGAGGTTATAGATGTGTCTACCCCGTCTCCTGACTGCAGAACAAGTTCGCATAGATTCAAGAGAAGACTTACTTCTGGTAGGTCTGAGATTATTGACTTGACCAAATCTCCAACGTTTATCCGATTGTAG

mRNA sequence

ATGAGGAAGAGGAAAGAGAAGCCAGAAATCTCTAAAACCATGGCTGTTGCAGAAGAAGAGAATGAAGACGGTGAAGAAGAAGAAAGGGGCAACGAAACGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCCTGCCCTCGTTACAAAGGCCATACCTATATCGGATTTACAGTGAACCCGAAACGTAGAATCCGGCAGCACAATGGCGAAATTAGATGCGGTGCGTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTTTTGTGCATTTATGGCTTCCCCACTAATGTCTCTGCTCTTCAGTTTGAATGGGCCTGGCAACATCCTAATGAGTCATTGGCTGTTAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCCAACAAAGTTAAACTTGCATACACTATGCTCACTCTTCCAGCTTGGTGCAGTTTGAATATCACTGTAAACTACTTCTCAACAAAGTACATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCTGAGCATATGAAGGTCCAAGTTTGCCCCATCAGTGAGCTTCCATGCTATTCTGAAGGAGATCAAGGTGTACTTGGAAATGAAGATGACTGGGAGTATAATAGAGAATATGAAGAAATTTGTAGTTTTCAAGTATATGGATCGATGAAAGAAGTTTCAAATGAAATTCCTCAAAAGTTAATGGATTTCCAGACAGGTACAGATGGAAAACCTAATGAATTGCATGGATGTGATAAAGAACTCGAGGACAATGAACGAGTGCCACCTTCTTCGTGTACTCCATCTGATAACGACGCAGATATGTCTTATGAACGCGATAAAGAACTCAAGAATGATGAACGAGAGCCACCTTCTTGTGCTCCGTCTCATATCATAGCGGGTATGTCTGGCACTGAAATCATCATCGAAGATGGAGAGGAAGACCAATTAGAAGGGAGGAGTGATATGAACTTGCATCAACAACCTGGTAGGAGGAATTTAACATCAGGAATGGCTACTAAAATGATCAAAGACTCAAGATGTAACAATGGGGTTCCTCCCGTTGAGTATGAGGTTATAGATGTGTCTACCCCGTCTCCTGACTGCAGAACAAGTTCGCATAGATTCAAGAGAAGACTTACTTCTGGTAGGTCTGAGATTATTGACTTGACCAAATCTCCAACGTTTATCCGATTGTAG

Coding sequence (CDS)

ATGAGGAAGAGGAAAGAGAAGCCAGAAATCTCTAAAACCATGGCTGTTGCAGAAGAAGAGAATGAAGACGGTGAAGAAGAAGAAAGGGGCAACGAAACGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCCTGCCCTCGTTACAAAGGCCATACCTATATCGGATTTACAGTGAACCCGAAACGTAGAATCCGGCAGCACAATGGCGAAATTAGATGCGGTGCGTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTTTTGTGCATTTATGGCTTCCCCACTAATGTCTCTGCTCTTCAGTTTGAATGGGCCTGGCAACATCCTAATGAGTCATTGGCTGTTAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCCAACAAAGTTAAACTTGCATACACTATGCTCACTCTTCCAGCTTGGTGCAGTTTGAATATCACTGTAAACTACTTCTCAACAAAGTACATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCTGAGCATATGAAGGTCCAAGTTTGCCCCATCAGTGAGCTTCCATGCTATTCTGAAGGAGATCAAGGTGTACTTGGAAATGAAGATGACTGGGAGTATAATAGAGAATATGAAGAAATTTGTAGTTTTCAAGTATATGGATCGATGAAAGAAGTTTCAAATGAAATTCCTCAAAAGTTAATGGATTTCCAGACAGGTACAGATGGAAAACCTAATGAATTGCATGGATGTGATAAAGAACTCGAGGACAATGAACGAGTGCCACCTTCTTCGTGTACTCCATCTGATAACGACGCAGATATGTCTTATGAACGCGATAAAGAACTCAAGAATGATGAACGAGAGCCACCTTCTTGTGCTCCGTCTCATATCATAGCGGGTATGTCTGGCACTGAAATCATCATCGAAGATGGAGAGGAAGACCAATTAGAAGGGAGGAGTGATATGAACTTGCATCAACAACCTGGTAGGAGGAATTTAACATCAGGAATGGCTACTAAAATGATCAAAGACTCAAGATGTAACAATGGGGTTCCTCCCGTTGAGTATGAGGTTATAGATGTGTCTACCCCGTCTCCTGACTGCAGAACAAGTTCGCATAGATTCAAGAGAAGACTTACTTCTGGTAGGTCTGAGATTATTGACTTGACCAAATCTCCAACGTTTATCCGATTGTAG

Protein sequence

MRKRKEKPEISKTMAVAEEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPISELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKPNELHGCDKELEDNERVPPSSCTPSDNDADMSYERDKELKNDEREPPSCAPSHIIAGMSGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNGVPPVEYEVIDVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL
Homology
BLAST of Lag0023420 vs. NCBI nr
Match: XP_038894793.1 (structure-specific endonuclease subunit SLX1 [Benincasa hispida])

HSP 1 Score: 664.1 bits (1712), Expect = 7.6e-187
Identity = 331/400 (82.75%), Postives = 360/400 (90.00%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVAEEE---NEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGF 60
           MRKRKE+PEISKTMAVAEEE   +ED EEEE  NE NGFFSCYLLASACPRYKGHTYIGF
Sbjct: 1   MRKRKERPEISKTMAVAEEEKEDDEDEEEEEGSNEVNGFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLP+HMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPKHMKVQV 180

Query: 181 CPISELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTD 240
            PI+ELPCYSEGDQ VL NEDDWEYNRE EEICSF+VYGSMKEVSNE+PQKLMD Q  TD
Sbjct: 181 SPINELPCYSEGDQCVLENEDDWEYNRECEEICSFRVYGSMKEVSNEVPQKLMDHQASTD 240

Query: 241 GKPNELHGCDKELEDNERVPPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHII 300
           G+P ELHGCDKE E+NE+VPPSSC PS  DA MSY+    D+EL+ DEREP SCAPS I+
Sbjct: 241 GRPRELHGCDKEFENNEQVPPSSCNPSYIDAGMSYDLHGCDEELEEDEREPASCAPSCIV 300

Query: 301 AGMSGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNGVPPVEYEV 360
             +S TEIII+DG+EDQ+EG + MNL QQ  R+NL+SG+A+++ K SRCN  +PPVEYEV
Sbjct: 301 TDISRTEIIIDDGDEDQIEG-TGMNLEQQLCRKNLSSGIASEISKVSRCNIELPPVEYEV 360

Query: 361 IDVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL 395
           IDVSTPSPDCRT+SHR KR++TSGRSEIIDLTKSPTFI+L
Sbjct: 361 IDVSTPSPDCRTNSHRVKRKVTSGRSEIIDLTKSPTFIQL 399

BLAST of Lag0023420 vs. NCBI nr
Match: XP_008457313.1 (PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo])

HSP 1 Score: 657.1 bits (1694), Expect = 9.3e-185
Identity = 331/402 (82.34%), Postives = 357/402 (88.81%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVA---EEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGF 60
           MRKRKEKPEI KTMA     EE+ +D EEEERGN  NGFFSCYLLASACPR+KGHTYIGF
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 CPISELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTD 240
            PI ELPCYSEGDQGVL NEDDWEY+RE EEICSFQVYGSMKEVSNE+PQKLMD+QTGTD
Sbjct: 181 SPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GKPNE-LHGCDKELEDNERVPPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHI 300
           G+P   L GCDKELE NE+VPPSSCTPS  D  MSY+    D+ L+NDERE  SC  S  
Sbjct: 241 GRPPPVLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCT 300

Query: 301 IAGMSGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNG-VPPVEY 360
           +AG S TEIII+DGEE+Q EG S MNL +QPGR+NLTSG+A+++ K SRC+NG VP VEY
Sbjct: 301 VAGTSRTEIIIDDGEENQFEG-SGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEY 360

Query: 361 EVIDVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL 395
           EVIDVSTPSPDCRTSSHRFKRR+TSG+SE+IDLTKSPTFI+L
Sbjct: 361 EVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 401

BLAST of Lag0023420 vs. NCBI nr
Match: XP_004153269.1 (structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus])

HSP 1 Score: 643.7 bits (1659), Expect = 1.1e-180
Identity = 326/399 (81.70%), Postives = 353/399 (88.47%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVAEEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVN 60
           MRKRKEKPEI KT    +EE ED EEEERGNE NGFFSCYLLASACPR+KGHTYIGFTVN
Sbjct: 1   MRKRKEKPEICKT---TDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPI 180
           ATFKSLSGVANKVKLAYTMLTLPAW  LNITVNYFSTK+MKNAAGCPSLPEHMKVQV PI
Sbjct: 121 ATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPI 180

Query: 181 SELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGK- 240
           +ELPCYSEGDQ +L NE DWEYNRE EEIC F+VYGSMKEVSNE+PQKLMD+QTGTDG+ 
Sbjct: 181 NELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRP 240

Query: 241 PNELHGCDKELEDNERVPPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHIIAG 300
           P+ L GCDKELE NE+VPPSSCTPS  D  MSY+    D+ L+NDERE  SC  S I+AG
Sbjct: 241 PHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAG 300

Query: 301 MSGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNG-VPPVEYEVI 360
            S TEI+I+D EE+QLEG S MNL +QPGR NLTSG+A+++ K SR NNG VP VEYEVI
Sbjct: 301 TSRTEIVIDDEEENQLEG-SSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVI 360

Query: 361 DVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL 395
           DVSTPSPDCRTSSHRFKRR+TSG+SE+IDLTKSPTFI+L
Sbjct: 361 DVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395

BLAST of Lag0023420 vs. NCBI nr
Match: KAA0060004.1 (Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ97262.1 Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa])

HSP 1 Score: 638.6 bits (1646), Expect = 3.4e-179
Identity = 317/382 (82.98%), Postives = 343/382 (89.79%), Query Frame = 0

Query: 18  EEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAW 77
           EE+ +D EEEERGN  NGFFSCYLLASACPR+KGHTYIGFTVNPKRRIRQHNGEIRCGAW
Sbjct: 8   EEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAW 67

Query: 78  RTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAY 137
           RTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAY
Sbjct: 68  RTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAY 127

Query: 138 TMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPISELPCYSEGDQGVLGNE 197
           TMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKVQV PI ELPCYSEGDQGVL NE
Sbjct: 128 TMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQGVLENE 187

Query: 198 DDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKPNE-LHGCDKELEDNERV 257
           DDWEY+RE EEICSFQVYGSMKEVSNE+PQKLMD+QTGTDG+P   L GCDKELE NE+V
Sbjct: 188 DDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRPPPVLRGCDKELETNEQV 247

Query: 258 PPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHIIAGMSGTEIIIEDGEEDQLE 317
           PPSSCTPS  D  MSY+    D+ L+NDERE  SC  S  +AG S TEIII+DGEE+Q E
Sbjct: 248 PPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCTVAGTSRTEIIIDDGEENQFE 307

Query: 318 GRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNG-VPPVEYEVIDVSTPSPDCRTSSHRFK 377
           G S MNL +QPGR+NLTSG+A+++ K SRC+NG VP VEYEVIDVSTPSPDCRTSSHRFK
Sbjct: 308 G-SGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFK 367

Query: 378 RRLTSGRSEIIDLTKSPTFIRL 395
           RR+TSG+SE+IDLTKSPTFI+L
Sbjct: 368 RRVTSGKSEMIDLTKSPTFIQL 388

BLAST of Lag0023420 vs. NCBI nr
Match: KAG7019098.1 (Structure-specific endonuclease subunit SLX1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 604.7 bits (1558), Expect = 5.5e-169
Identity = 306/395 (77.47%), Postives = 340/395 (86.08%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVAEEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVN 60
           MR+RKEKPEI KTMAVAEE+ ED E+EERGNE NGFFSCYLLASACPRYKGHTYIGFTVN
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDEDEERGNEENGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPI 180
           ATFKSLSG+ANK+KLAYTMLTLPAWCSLNITVNYFSTKYM NAAGCPSLP HM+VQV PI
Sbjct: 121 ATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQVSPI 180

Query: 181 SELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKP 240
            ELPCYSEGD+ VL NEDDWEYNREYEE+C  QV+  M EV NEIPQ LMD+QTGTDG+P
Sbjct: 181 DELPCYSEGDRCVLENEDDWEYNREYEEVCDSQVHDLMTEVPNEIPQTLMDYQTGTDGRP 240

Query: 241 NELHGCDKELEDNERVPPSSCTPSDNDADMSYERDKELKN-DEREPPSCAPSHIIAGMSG 300
           +EL G D+ELEDNE++P SSCTPS  D  +SY    +L+  DE++P  CA   I+ G   
Sbjct: 241 HELRGSDEELEDNEQMPHSSCTPSYIDVSVSY----DLRGCDEQKPALCAQPCIVEGR-- 300

Query: 301 TEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNGVPPVEYEVIDVST 360
           TEIII+DGEE+ LEG S   L QQPGR+NL SG+AT++   S  + G+PPVE EVIDVST
Sbjct: 301 TEIIIDDGEENHLEG-SGTKLQQQPGRKNL-SGIATEI---SEVSYGLPPVENEVIDVST 360

Query: 361 PSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL 395
           PSPDCRTSS++FKRR+ +GRSEIIDLTKSPTFI+L
Sbjct: 361 PSPDCRTSSYKFKRRVITGRSEIIDLTKSPTFIQL 384

BLAST of Lag0023420 vs. ExPASy Swiss-Prot
Match: Q8BX32 (Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-29
Identity = 66/162 (40.74%), Postives = 92/162 (56.79%), Query Frame = 0

Query: 36  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 95
           FF  YLL    PR++G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL I+GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLIIHGF 69

Query: 96  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 155
           P+ V+AL+FEWAWQHP  S  +       +S +  A  +++   ML +P W  L +T+ +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEAAFAFHLRVLAHMLRVPPWVRLPLTLRW 129

Query: 156 FSTKYMKNAAGCPSLPEHMKVQVCP------ISELPCYSEGD 191
               +      CP+ P HM +   P      + + P  SE D
Sbjct: 130 LRPDFRHEL--CPAPPAHMPIAFGPPPPQPLVPKRPAVSEAD 169

BLAST of Lag0023420 vs. ExPASy Swiss-Prot
Match: Q5PQP5 (Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.2e-29
Identity = 65/162 (40.12%), Postives = 91/162 (56.17%), Query Frame = 0

Query: 36  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 95
           FF  YLL    PR++G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL ++GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLILHGF 69

Query: 96  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 155
           P+ V+AL+FEWAWQHP  S  +       +S +     +++   ML +P W  L +TV +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEASFTFHLRVLAHMLRVPPWVRLPLTVRW 129

Query: 156 FSTKYMKNAAGCPSLPEHMKVQVCP------ISELPCYSEGD 191
               +      CP+ P HM +   P      + + P  SE D
Sbjct: 130 LRPDFRHEL--CPAPPPHMPIAFGPPPPQPLVPKRPAASEAD 169

BLAST of Lag0023420 vs. ExPASy Swiss-Prot
Match: Q32PI0 (Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.3e-27
Identity = 60/145 (41.38%), Postives = 85/145 (58.62%), Query Frame = 0

Query: 36  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 95
           FF  YLL    PR++G  Y+GFTVNP RR++QHN G  + GAWRT  + PWEMVL ++GF
Sbjct: 6   FFGVYLLYCLNPRHRGRVYVGFTVNPARRVQQHNGGRKKGGAWRTSGRGPWEMVLIVHGF 65

Query: 96  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 155
           P+ V+AL+FEWAWQHP  S  +       +  +  A  +++   ML  P W  L +T+ +
Sbjct: 66  PSAVAALRFEWAWQHPQASRRLTHVGRRLRGEATFAFHLRVLAHMLRAPPWVRLPLTLRW 125

Query: 156 FSTKYMKNAAGCPSLPEHMKVQVCP 180
               + ++   CP  P HM +   P
Sbjct: 126 LRADFRQDL--CPPPPPHMPLAFGP 148

BLAST of Lag0023420 vs. ExPASy Swiss-Prot
Match: Q0IH86 (Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.9e-27
Identity = 66/176 (37.50%), Postives = 96/176 (54.55%), Query Frame = 0

Query: 32  ETNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLC 91
           E  GF+  YLL    P+YKG  YIGFTVNP+RRI+QHN G+ + GAW+T  + PW+MVL 
Sbjct: 4   EVEGFYGVYLLFCTNPKYKGRIYIGFTVNPERRIQQHNGGKHKGGAWKTSGRGPWDMVLI 63

Query: 92  IYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNI 151
           ++GFP +++AL+FEWAWQHP+ S  +       K  S     + +   ML +  W  L +
Sbjct: 64  VHGFPNDIAALRFEWAWQHPHVSRRLTHVPRKTKKQSSFDFHLLVLCHMLRVAPWNRLPL 123

Query: 152 TVNYFSTKYMKNAAGCPSLPEHMKVQVCPISELPC-YSEGDQGVLGNEDDWEYNRE 206
           T+ +   +Y +        P HM +    +   P    E ++G LG     E  +E
Sbjct: 124 TLRWLRQEYRRELPLLLQPPLHMPLAFGQVRARPIPKGEKEKGRLGENRAEETEQE 179

BLAST of Lag0023420 vs. ExPASy Swiss-Prot
Match: Q9BQ83 (Structure-specific endonuclease subunit SLX1 OS=Homo sapiens OX=9606 GN=SLX1A PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 9.2e-26
Identity = 58/145 (40.00%), Postives = 84/145 (57.93%), Query Frame = 0

Query: 36  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 95
           FF  YLL    PRY+G  Y+GFTVN  RR++QHN G  + GAWRT  + PWEMVL ++GF
Sbjct: 13  FFGVYLLYCLNPRYRGRVYVGFTVNTARRVQQHNGGRKKGGAWRTSGRGPWEMVLVVHGF 72

Query: 96  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 155
           P++V+AL+FEWAWQHP+ S  +       +  +  A  +++   ML  P W  L +T+ +
Sbjct: 73  PSSVAALRFEWAWQHPHASRRLAHVGPRLRGETAFAFHLRVLAHMLRAPPWARLPLTLRW 132

Query: 156 FSTKYMKNAAGCPSLPEHMKVQVCP 180
                 ++   C   P H+ +   P
Sbjct: 133 VRPDLRQDL--CLPPPPHVPLAFGP 155

BLAST of Lag0023420 vs. ExPASy TrEMBL
Match: A0A1S3C574 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=LOC103497035 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.5e-185
Identity = 331/402 (82.34%), Postives = 357/402 (88.81%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVA---EEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGF 60
           MRKRKEKPEI KTMA     EE+ +D EEEERGN  NGFFSCYLLASACPR+KGHTYIGF
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 CPISELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTD 240
            PI ELPCYSEGDQGVL NEDDWEY+RE EEICSFQVYGSMKEVSNE+PQKLMD+QTGTD
Sbjct: 181 SPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GKPNE-LHGCDKELEDNERVPPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHI 300
           G+P   L GCDKELE NE+VPPSSCTPS  D  MSY+    D+ L+NDERE  SC  S  
Sbjct: 241 GRPPPVLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCT 300

Query: 301 IAGMSGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNG-VPPVEY 360
           +AG S TEIII+DGEE+Q EG S MNL +QPGR+NLTSG+A+++ K SRC+NG VP VEY
Sbjct: 301 VAGTSRTEIIIDDGEENQFEG-SGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEY 360

Query: 361 EVIDVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL 395
           EVIDVSTPSPDCRTSSHRFKRR+TSG+SE+IDLTKSPTFI+L
Sbjct: 361 EVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 401

BLAST of Lag0023420 vs. ExPASy TrEMBL
Match: A0A0A0M0Y6 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G533630 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.2e-181
Identity = 326/399 (81.70%), Postives = 353/399 (88.47%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVAEEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVN 60
           MRKRKEKPEI KT    +EE ED EEEERGNE NGFFSCYLLASACPR+KGHTYIGFTVN
Sbjct: 1   MRKRKEKPEICKT---TDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPI 180
           ATFKSLSGVANKVKLAYTMLTLPAW  LNITVNYFSTK+MKNAAGCPSLPEHMKVQV PI
Sbjct: 121 ATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPI 180

Query: 181 SELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGK- 240
           +ELPCYSEGDQ +L NE DWEYNRE EEIC F+VYGSMKEVSNE+PQKLMD+QTGTDG+ 
Sbjct: 181 NELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRP 240

Query: 241 PNELHGCDKELEDNERVPPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHIIAG 300
           P+ L GCDKELE NE+VPPSSCTPS  D  MSY+    D+ L+NDERE  SC  S I+AG
Sbjct: 241 PHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAG 300

Query: 301 MSGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNG-VPPVEYEVI 360
            S TEI+I+D EE+QLEG S MNL +QPGR NLTSG+A+++ K SR NNG VP VEYEVI
Sbjct: 301 TSRTEIVIDDEEENQLEG-SSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVI 360

Query: 361 DVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTFIRL 395
           DVSTPSPDCRTSSHRFKRR+TSG+SE+IDLTKSPTFI+L
Sbjct: 361 DVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395

BLAST of Lag0023420 vs. ExPASy TrEMBL
Match: A0A5D3BBR0 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00560 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.7e-179
Identity = 317/382 (82.98%), Postives = 343/382 (89.79%), Query Frame = 0

Query: 18  EEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAW 77
           EE+ +D EEEERGN  NGFFSCYLLASACPR+KGHTYIGFTVNPKRRIRQHNGEIRCGAW
Sbjct: 8   EEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAW 67

Query: 78  RTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAY 137
           RTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAY
Sbjct: 68  RTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAY 127

Query: 138 TMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPISELPCYSEGDQGVLGNE 197
           TMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKVQV PI ELPCYSEGDQGVL NE
Sbjct: 128 TMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQGVLENE 187

Query: 198 DDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKPNE-LHGCDKELEDNERV 257
           DDWEY+RE EEICSFQVYGSMKEVSNE+PQKLMD+QTGTDG+P   L GCDKELE NE+V
Sbjct: 188 DDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRPPPVLRGCDKELETNEQV 247

Query: 258 PPSSCTPSDNDADMSYER---DKELKNDEREPPSCAPSHIIAGMSGTEIIIEDGEEDQLE 317
           PPSSCTPS  D  MSY+    D+ L+NDERE  SC  S  +AG S TEIII+DGEE+Q E
Sbjct: 248 PPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCTVAGTSRTEIIIDDGEENQFE 307

Query: 318 GRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNG-VPPVEYEVIDVSTPSPDCRTSSHRFK 377
           G S MNL +QPGR+NLTSG+A+++ K SRC+NG VP VEYEVIDVSTPSPDCRTSSHRFK
Sbjct: 308 G-SGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFK 367

Query: 378 RRLTSGRSEIIDLTKSPTFIRL 395
           RR+TSG+SE+IDLTKSPTFI+L
Sbjct: 368 RRVTSGKSEMIDLTKSPTFIQL 388

BLAST of Lag0023420 vs. ExPASy TrEMBL
Match: A0A6J1DNH4 (Structure-specific endonuclease subunit SLX1 homolog OS=Momordica charantia OX=3673 GN=LOC111022012 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 5.2e-165
Identity = 311/399 (77.94%), Postives = 330/399 (82.71%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVAEEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVN 60
           MRKRKEK EISK M VAEEENED   EERGNE+NGFFSCYLLASACPRYKGHTYIGFTVN
Sbjct: 1   MRKRKEKQEISKPMVVAEEENED---EERGNESNGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEI CGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIGCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPI 180
           ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYM NAAGCPSLPEHMKVQV PI
Sbjct: 121 ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPEHMKVQVSPI 180

Query: 181 SELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKP 240
            +LPCYSEGDQGV  NEDD EYNREYEE+CSF+VYGS KEV NEIPQKL D         
Sbjct: 181 DDLPCYSEGDQGVAENEDDLEYNREYEEVCSFRVYGSTKEVPNEIPQKLTD--------- 240

Query: 241 NELHGCDKELEDNERVPPSSCTPSDNDADMSYE---RDKELKNDEREPPSCAPSHIIAGM 300
               GCDKELED+ER PPSSC+PS     MS++    +KEL++ +R PPSCAPS+ +AG 
Sbjct: 241 ----GCDKELEDSEREPPSSCSPS--YVGMSHDMHGHNKELEDYKRVPPSCAPSYTVAGR 300

Query: 301 SGTEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNGVPPVEYEVIDV 360
           S TEIIIEDGEEDQ EG + +NL Q+PG  N        M K  RCN G PP EYEVIDV
Sbjct: 301 SETEIIIEDGEEDQFEG-NGVNLQQKPGSTN-------SMSKVGRCNIGYPPSEYEVIDV 360

Query: 361 STPSPDCRTSSH-RFKRRLTS-GRSEIIDLTKSPTFIRL 395
            TPSPDCRTSS+ RFKRR+TS   SEIIDLTKSPTFIRL
Sbjct: 361 CTPSPDCRTSSNSRFKRRITSFVGSEIIDLTKSPTFIRL 373

BLAST of Lag0023420 vs. ExPASy TrEMBL
Match: A0A6J1I681 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 9.2e-162
Identity = 290/376 (77.13%), Postives = 323/376 (85.90%), Query Frame = 0

Query: 1   MRKRKEKPEISKTMAVAEEENEDGEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVN 60
           MR+RKEKPEI KTMAVAEE+ ED E+EERGNE NGFFSCYLLASACPRYKGHTYIGFTVN
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPI 180
           ATFKSLSG+ANK+KLAYTMLTLPAWCSLNITVNYFSTKYM NAAGCPSLP HM+VQ+ PI
Sbjct: 121 ATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQISPI 180

Query: 181 SELPCYSEGDQGVLGNEDDWEYNREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKP 240
            ELPCYSEGD+ VL NE+DWEYN+EYEE+CS QV+G M EV NEIPQ LMD+ TGTDG+P
Sbjct: 181 DELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTDGRP 240

Query: 241 NELHGCDKELEDNERVPPSSCTPSDNDADMSYERDKELKN-DEREPPSCAPSHIIAGMSG 300
           +EL G D+EL+DNE+VP SSCTPS  D  +SY    +L+  DE+EP  CA    + G   
Sbjct: 241 HELRGSDEELDDNEQVPHSSCTPSYIDVGVSY----DLRGCDEQEPALCAQPCTVEGW-- 300

Query: 301 TEIIIEDGEEDQLEGRSDMNLHQQPGRRNLTSGMATKMIKDSRCNNGVPPVEYEVIDVST 360
           TEIII+DGEE+QLEG S M L QQPGR+   SG+AT++   S+ + G+PPVEYEVIDVST
Sbjct: 301 TEIIIDDGEENQLEG-SGMKLQQQPGRKKNLSGIATEI---SKVSYGLPPVEYEVIDVST 360

Query: 361 PSPDCRTSSHRFKRRL 376
           PSP CRTSSH+FKRRL
Sbjct: 361 PSPVCRTSSHKFKRRL 366

BLAST of Lag0023420 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 275.8 bits (704), Expect = 5.4e-74
Identity = 175/378 (46.30%), Postives = 215/378 (56.88%), Query Frame = 0

Query: 24  GEEEERGNETNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKR 83
           GE+   G +  GFF+CYLL S  PR+KG TYIGFTVNP+RRIRQHNGEI  GAWRTK+KR
Sbjct: 15  GEDGVTGKDGKGFFACYLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKR 74

Query: 84  PWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLP 143
           PWEMVLCIYGFPTNVSALQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LP
Sbjct: 75  PWEMVLCIYGFPTNVSALQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLP 134

Query: 144 AWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVCPISELPCYSE-GDQGVLGNEDDWEY 203
           AW SLN+TVNYFS+KY  +    PSLP HMKVQVC + +L  +++  D     +E+  E 
Sbjct: 135 AWNSLNLTVNYFSSKYAHHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEV 194

Query: 204 NREYEEICSFQVYGSMKEVSNEIPQKLMDFQTGTDGKPNELHGCDKELEDNERVPPSSCT 263
           N E ++           + S+ + Q          G  N     D+  E   + P +   
Sbjct: 195 NEEDDD--------DDDDDSSNLSQ---------PGNSNTSSSDDRHFE-KAKEPVTVFD 254

Query: 264 PSDNDADMSYERDKELKNDEREPPSCAPSHIIAG-MSGTEIIIEDGEEDQLEGRSDMNLH 323
             D  A+ S         DE E      SHI  G +  TE   E    D+L   +   L 
Sbjct: 255 EEDRLANFSGFG----LLDEEETVEDEVSHITVGSIRATEKEPETVFNDRLASFTCFGLV 314

Query: 324 Q--QPGRRNLTSGMATKMIKDSR-----CNNGVPPVEYEVIDVSTPSPDCRTSSHRFKRR 383
           +  +    + T G    M K+ R       +    V+ EVID+ TPSP CR  S   +RR
Sbjct: 315 EIVEDEVSHGTIGSTEAMEKECRKRRNHITSTTTEVDVEVIDLMTPSPSCRAGSSMKRRR 366

Query: 384 LTSGRSEIIDLTKSPTFI 393
           +    SE IDLT SP FI
Sbjct: 375 V----SEFIDLTMSPNFI 366

BLAST of Lag0023420 vs. TAIR 10
Match: AT2G30350.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-35
Identity = 116/301 (38.54%), Postives = 150/301 (49.83%), Query Frame = 0

Query: 101 LQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYM 160
           LQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LPAW SLN+TVNYFS+KY 
Sbjct: 58  LQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLPAWNSLNLTVNYFSSKYA 117

Query: 161 KNAAGCPSLPEHMKVQVCPISELPCYSE-GDQGVLGNEDDWEYNREYEEICSFQVYGSMK 220
            +    PSLP HMKVQVC + +L  +++  D     +E+  E N E ++           
Sbjct: 118 HHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEVNEEDDD--------DDD 177

Query: 221 EVSNEIPQKLMDFQTGTDGKPNELHGCDKELEDNERVPPSSCTPSDNDADMSYERDKELK 280
           + S+ + Q          G  N     D+  E   + P +     D  A+ S        
Sbjct: 178 DDSSNLSQ---------PGNSNTSSSDDRHFE-KAKEPVTVFDEEDRLANFSGFG----L 237

Query: 281 NDEREPPSCAPSHIIAG-MSGTEIIIEDGEEDQLEGRSDMNLHQ--QPGRRNLTSGMATK 340
            DE E      SHI  G +  TE   E    D+L   +   L +  +    + T G    
Sbjct: 238 LDEEETVEDEVSHITVGSIRATEKEPETVFNDRLASFTCFGLVEIVEDEVSHGTIGSTEA 297

Query: 341 MIKDSR-----CNNGVPPVEYEVIDVSTPSPDCRTSSHRFKRRLTSGRSEIIDLTKSPTF 393
           M K+ R       +    V+ EVID+ TPSP CR  S   +RR+    SE IDLT SP F
Sbjct: 298 MEKECRKRRNHITSTTTEVDVEVIDLMTPSPSCRAGSSMKRRRV----SEFIDLTMSPNF 332

BLAST of Lag0023420 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 56.2 bits (134), Expect = 6.8e-08
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 37  FSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPT 96
           +S YL+ S     K  TY+G T +  RR++QHNGEIR GA  +   RPW     I GF  
Sbjct: 54  WSVYLILSTTEPIK--TYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTC 113

Query: 97  NVSALQFEWAWQ 109
              A  FE  W+
Sbjct: 114 LSQASSFESKWK 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894793.17.6e-18782.75structure-specific endonuclease subunit SLX1 [Benincasa hispida][more]
XP_008457313.19.3e-18582.34PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo][more]
XP_004153269.11.1e-18081.70structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus][more]
KAA0060004.13.4e-17982.98Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ9726... [more]
KAG7019098.15.5e-16977.47Structure-specific endonuclease subunit SLX1, partial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
Q8BX321.8e-2940.74Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b P... [more]
Q5PQP55.2e-2940.12Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Sl... [more]
Q32PI01.3e-2741.38Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2... [more]
Q0IH862.9e-2737.50Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a ... [more]
Q9BQ839.2e-2640.00Structure-specific endonuclease subunit SLX1 OS=Homo sapiens OX=9606 GN=SLX1A PE... [more]
Match NameE-valueIdentityDescription
A0A1S3C5744.5e-18582.34Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=... [more]
A0A0A0M0Y65.2e-18181.70Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 ... [more]
A0A5D3BBR01.7e-17982.98Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa... [more]
A0A6J1DNH45.2e-16577.94Structure-specific endonuclease subunit SLX1 homolog OS=Momordica charantia OX=3... [more]
A0A6J1I6819.2e-16277.13Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT2G30350.25.4e-7446.30Excinuclease ABC, C subunit, N-terminal [more]
AT2G30350.12.2e-3538.54Excinuclease ABC, C subunit, N-terminal [more]
AT5G43210.16.8e-0841.67Excinuclease ABC, C subunit, N-terminal [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000305GIY-YIG endonucleasePFAMPF01541GIY-YIGcoord: 39..112
e-value: 1.7E-12
score: 47.4
IPR000305GIY-YIG endonucleasePROSITEPS50164GIY_YIGcoord: 35..117
score: 17.191767
IPR035901GIY-YIG endonuclease superfamilyGENE3D3.40.1440.10coord: 33..135
e-value: 3.4E-24
score: 86.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..255
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..293
NoneNo IPR availablePANTHERPTHR20208:SF10STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 18..389
NoneNo IPR availablePANTHERPTHR20208STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 18..389
NoneNo IPR availableCDDcd10455GIY-YIG_SLX1coord: 36..110
e-value: 4.9093E-54
score: 171.647
IPR027520Structure-specific endonuclease subunit Slx1HAMAPMF_03100Endonuc_su_Slx1coord: 32..381
score: 23.670719

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0023420.1Lag0023420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0051569 regulation of histone H3-K4 methylation
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006281 DNA repair
cellular_component GO:0016593 Cdc73/Paf1 complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0033557 Slx1-Slx4 complex
molecular_function GO:0017108 5'-flap endonuclease activity
molecular_function GO:0008821 crossover junction endodeoxyribonuclease activity
molecular_function GO:0000993 RNA polymerase II complex binding