Lag0023332 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0023332
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Phosphomethylpyrimidine synthase
Location	chr7: 47263837 .. 47266950 (+)
RNA-Seq Expression	Lag0023332
Synteny	Lag0023332

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCAGTTCATGCTTCCGTAACACCAGCTGTTGGTAAGAGTGGGAACCGTTCCTACCCTACGAAATCACTTAATACTGCCTTCCTTCCTGGGTTTGACGTGGTTGGACGTGCTTCCGGTACGTGCAAGGACTTGTACTCTTCATCTATTACCTTAACTCCTAGAGCCACTTTAACCTTTGAACCTCCGGAAACCAGTATGGAGAAGGCCAAAACCAAGAAACATACAGTTGACCCTTCATCTCCTGATTTTCTGCCGCTTCCTTCATTTGAACAATGTTTCCCAAAAAGCACAAAAGAACACAAGTGAGTCAACTCATTGGAGTATTGTTTTTTCATAGTACTTTTGCAAAACTAGATAGACGATTTTTTTTTTTTTTTGATAGGAAGATAGACGATGGTCATAAACTGATATCATATACTTTTGCCATTATTGATGCATAATTTGTTATGCAGGGAAGTTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGACGGGTTCATTTATCTGGTGATGAACCAAACTTTGACAATTATGACACTAGTGGTCCTCAAAACATCAGCCCCCGCATTGGTAAGATGATCATATTTTAACTGTTACTTGAAAAGACTAGTTTTTGCTATATTTGTGTTATAGCAGATATTGGTTTTGTTTATTATTGTTTTTTATTTTTTTGAAAAAATGTTTATTATTTTTTAATTGTCCATGAATGTATATTTTTAGTCTAAATATAATTTTTGTGAAAACTGTTTGGAGTCTGACTTTGTTGCTATAAATCATTTTATTATTTTTATATAGTGAACGGAGTTCTTCCATTTGGATATACATGTCTGTTATTTTAAATCTGCAGCATAAGACAGCTATTTTTTTCCCTAAACGTTAGATACCAACTAGTGTGAAACTGAAAACTTTTAGCTGTGGGCCTATCTGATTCCATTGAGAAACATAAATGGCGGCAACTTGCCTGCTTTCTATGATGGAAACTAAGTCCCTTTGAGATTTCGTTACATTTTTTATAATCCAATTTCTATACATGGATGCTTCCTGACTTCCATGATGCAACAATGCAAATTAGTAATGGCAATTTTTTTCTTTGGTATCCTTTTCTGGTTTACTTGTTACCTTTGTCTTTCAATTTACATTGTTACTGACAATCAACAATGTTATATAGGATTGCCTAAACTTCGGAAGGATTGGGTTGATAGGAGGGACAAATTAGGCGCACCAAGATACACACAAATGTATTATGCCAAACAAGGGATCATAACCGAGGAAATGTTGTATTGTGCCACTCGTGAGAAGCTTGACCCGGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGCGGGCAATCATCCCTTCCAACAAGAAGCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCAAGTTCTATTGAAGAGGAAGTTTATAAAGTCCAATGGGCAACTATGTGGGGAGCCGATACTGTCATGGACCTCTCTACTGGTCGCCACATACACGAGACTCGTGAGTGGATATTGCGTAATTCAGCCGTACCAGTAGGGACTGTTCCCATATATCAGGCTCTTGAAAAAGTGAATGGAATAGCTGAAAACCTCACCTGGGAGATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGCGTAGACTATTTTACTATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACGGGAATTGTATCACGTGGAGGATCCATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAACTTTGCTTACGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCTTATCAATTGGTGATGGGCTAAGACCTGGTTCAATTTATGATGCCAATGACACTGCTCAATTTGCAGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAGTGAATAGGAGTCTCTCTTGCTTACGTACATGCATGTGCTCACAATCACACCTATGTTAGTTATATGTATTTGAGAGCAAACCCATTTACTGCTAGTTATATGCATTTGACATTTGCAGCTGAAGCGTTGGGAAATAGGAACCTCTCAAAGCAGTATCTTATGATTTCATCACCCTATAAATTTCTTTGCCCTGGTTTGGTTGAGAGCTCTAATTTATAATATAGGACACTTACTCTGAAGTAGCAGATTCTTTTCGCACAATTAGTTTTATGGGAATGCACATTAGGAATATTGAACTAAGATTATCATTATTGTTGATGACTCATAAGTGTAATTATTGTCTGTTATTTTCATTTTAGGAACTAGAAATGTTATTTCTTTCGTGTTACTAACTAGTGGCCACATATTTGAGTTTCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAGGCACCTTTTTACACTCTTGGCCCTCTGACTACGGATGTAGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCTGCCAATATTGGGGCTCTTGGCACAGCCCTCCTCTGTTACGTTACACCGAAAGAGCATCTAGGATTGCCAAATCGAGATGATGTGAAGGCTGGAGTAATAGCGTATAAGATAGCTGCTCATGCAGCTGATCTAGCCAAAGGTCACCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCCAGATTCGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTCCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCCCACTTCTGCTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGGAAGTATGCTGAGGAGCACGGGTACGGGAGTGCAGAGGAAGCTCTGAAGCAAGGGATGGATGCTATGAGTGCTGAGTTTTTGGCTGCAAAGAAAACCATTAGTGGTGAACAGCATGGTGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACTTGGACTCGCTGAAGAGGTGA

mRNA sequence

ATGGCGTCAGTTCATGCTTCCGTAACACCAGCTGTTGGTAAGAGTGGGAACCGTTCCTACCCTACGAAATCACTTAATACTGCCTTCCTTCCTGGGTTTGACGTGGTTGGACGTGCTTCCGGTACGTGCAAGGACTTGTACTCTTCATCTATTACCTTAACTCCTAGAGCCACTTTAACCTTTGAACCTCCGGAAACCAGTATGGAGAAGGCCAAAACCAAGAAACATACAGTTGACCCTTCATCTCCTGATTTTCTGCCGCTTCCTTCATTTGAACAATGTTTCCCAAAAAGCACAAAAGAACACAAGGAAGTTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGACGGGTTCATTTATCTGGTGATGAACCAAACTTTGACAATTATGACACTAGTGGTCCTCAAAACATCAGCCCCCGCATTGGATTGCCTAAACTTCGGAAGGATTGGGTTGATAGGAGGGACAAATTAGGCGCACCAAGATACACACAAATGTATTATGCCAAACAAGGGATCATAACCGAGGAAATGTTGTATTGTGCCACTCGTGAGAAGCTTGACCCGGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGCGGGCAATCATCCCTTCCAACAAGAAGCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCAAGTTCTATTGAAGAGGAAGTTTATAAAGTCCAATGGGCAACTATGTGGGGAGCCGATACTGTCATGGACCTCTCTACTGGTCGCCACATACACGAGACTCGTGAGTGGATATTGCGTAATTCAGCCGTACCAGTAGGGACTGTTCCCATATATCAGGCTCTTGAAAAAGTGAATGGAATAGCTGAAAACCTCACCTGGGAGATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGCGTAGACTATTTTACTATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACGGGAATTGTATCACGTGGAGGATCCATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAACTTTGCTTACGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCTTATCAATTGGTGATGGGCTAAGACCTGGTTCAATTTATGATGCCAATGACACTGCTCAATTTGCAGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAGGCACCTTTTTACACTCTTGGCCCTCTGACTACGGATGTAGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCTGCCAATATTGGGGCTCTTGGCACAGCCCTCCTCTGTTACGTTACACCGAAAGAGCATCTAGGATTGCCAAATCGAGATGATGTGAAGGCTGGAGTAATAGCGTATAAGATAGCTGCTCATGCAGCTGATCTAGCCAAAGGTCACCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCCAGATTCGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTCCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCCCACTTCTGCTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGGAAGTATGCTGAGGAGCACGGGTACGGGAGTGCAGAGGAAGCTCTGAAGCAAGGGATGGATGCTATGAGTGCTGAGTTTTTGGCTGCAAAGAAAACCATTAGTGGTGAACAGCATGGTGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACTTGGACTCGCTGAAGAGGTGA

Coding sequence (CDS)

ATGGCGTCAGTTCATGCTTCCGTAACACCAGCTGTTGGTAAGAGTGGGAACCGTTCCTACCCTACGAAATCACTTAATACTGCCTTCCTTCCTGGGTTTGACGTGGTTGGACGTGCTTCCGGTACGTGCAAGGACTTGTACTCTTCATCTATTACCTTAACTCCTAGAGCCACTTTAACCTTTGAACCTCCGGAAACCAGTATGGAGAAGGCCAAAACCAAGAAACATACAGTTGACCCTTCATCTCCTGATTTTCTGCCGCTTCCTTCATTTGAACAATGTTTCCCAAAAAGCACAAAAGAACACAAGGAAGTTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGACGGGTTCATTTATCTGGTGATGAACCAAACTTTGACAATTATGACACTAGTGGTCCTCAAAACATCAGCCCCCGCATTGGATTGCCTAAACTTCGGAAGGATTGGGTTGATAGGAGGGACAAATTAGGCGCACCAAGATACACACAAATGTATTATGCCAAACAAGGGATCATAACCGAGGAAATGTTGTATTGTGCCACTCGTGAGAAGCTTGACCCGGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGCGGGCAATCATCCCTTCCAACAAGAAGCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCAAGTTCTATTGAAGAGGAAGTTTATAAAGTCCAATGGGCAACTATGTGGGGAGCCGATACTGTCATGGACCTCTCTACTGGTCGCCACATACACGAGACTCGTGAGTGGATATTGCGTAATTCAGCCGTACCAGTAGGGACTGTTCCCATATATCAGGCTCTTGAAAAAGTGAATGGAATAGCTGAAAACCTCACCTGGGAGATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGCGTAGACTATTTTACTATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACGGGAATTGTATCACGTGGAGGATCCATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAACTTTGCTTACGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCTTATCAATTGGTGATGGGCTAAGACCTGGTTCAATTTATGATGCCAATGACACTGCTCAATTTGCAGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAGGCACCTTTTTACACTCTTGGCCCTCTGACTACGGATGTAGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCTGCCAATATTGGGGCTCTTGGCACAGCCCTCCTCTGTTACGTTACACCGAAAGAGCATCTAGGATTGCCAAATCGAGATGATGTGAAGGCTGGAGTAATAGCGTATAAGATAGCTGCTCATGCAGCTGATCTAGCCAAAGGTCACCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCCAGATTCGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTCCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCCCACTTCTGCTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGGAAGTATGCTGAGGAGCACGGGTACGGGAGTGCAGAGGAAGCTCTGAAGCAAGGGATGGATGCTATGAGTGCTGAGTTTTTGGCTGCAAAGAAAACCATTAGTGGTGAACAGCATGGTGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACTTGGACTCGCTGAAGAGGTGA

Protein sequence

MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLTFEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRRVHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSAEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR

Homology

[Close]

BLAST of Lag0023332 vs. NCBI nr
Match: XP_023519726.1 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1302.0 bits (3368), Expect = 0.0e+00
Identity = 628/647 (97.06%), Postives = 634/647 (97.99%), Query Frame = 0


Query: 1    MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
            MASVHA++TPAVGKSGN SYPTKSLNTAFLPGFDVVGR SG CKD Y SSITLTPRATLT
Sbjct: 591  MASVHATITPAVGKSGNHSYPTKSLNTAFLPGFDVVGRVSGACKDSYPSSITLTPRATLT 650

Query: 61   FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
             EP ETS  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR
Sbjct: 651  SEPMETSTVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 710

Query: 121  VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
            VHLSGDEPNFDNYDTSGPQNISPR GLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE
Sbjct: 711  VHLSGDEPNFDNYDTSGPQNISPRTGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 770

Query: 181  MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
            ML+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASS
Sbjct: 771  MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASS 830

Query: 241  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
            IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 831  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 890

Query: 301  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
            NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 891  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 950

Query: 361  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
            ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 951  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 1010

Query: 421  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
            QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 1011 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 1070

Query: 481  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
            GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 1071 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 1130

Query: 541  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
            WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 1131 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 1190

Query: 601  AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 1191 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 1237

[Close]

BLAST of Lag0023332 vs. NCBI nr
Match: KAG6583379.1 (Phosphomethylpyrimidine synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1300.8 bits (3365), Expect = 0.0e+00
Identity = 627/647 (96.91%), Postives = 634/647 (97.99%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGK+GN SYPTKSLNTAFLPGFDVVGR SG CKD Y SSITLTPRATLT
Sbjct: 1   MASVHATITPAVGKNGNHSYPTKSLNTAFLPGFDVVGRVSGACKDSYPSSITLTPRATLT 60

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
            EP ETS  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR
Sbjct: 61  SEPMETSTVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR GLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRTGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 601 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 647

[Close]

BLAST of Lag0023332 vs. NCBI nr
Match: XP_022964789.1 (phosphomethylpyrimidine synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1297.3 bits (3356), Expect = 0.0e+00
Identity = 624/647 (96.45%), Postives = 633/647 (97.84%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGKSGN SYPTKSLNTAFLPGFDVVGR SG CK+ Y SSITLTPRATLT
Sbjct: 1   MASVHATITPAVGKSGNHSYPTKSLNTAFLPGFDVVGRVSGACKNSYPSSITLTPRATLT 60

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
            EP E S  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR
Sbjct: 61  SEPMENSTVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR GLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRTGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVP+YQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPVYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           Q+MNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QIMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 601 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 647

[Close]

BLAST of Lag0023332 vs. NCBI nr
Match: XP_022970663.1 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita maxima] >XP_022970664.1 phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 621/647 (95.98%), Postives = 634/647 (97.99%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGKSGN SYPTKSLN+AFLPGFD+VGR SG CKD Y SSIT+TPRATLT
Sbjct: 46  MASVHATITPAVGKSGNHSYPTKSLNSAFLPGFDMVGRISGACKDSYPSSITITPRATLT 105

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
            EP ETS  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVH+ETGHVLKVPFRR
Sbjct: 106 SEPMETSRVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 165

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR GLP+LRKDWVDRRDKLGAPRYTQMYYA+QGIITEE
Sbjct: 166 VHLSGDEPNFDNYDTSGPQNISPRTGLPELRKDWVDRRDKLGAPRYTQMYYARQGIITEE 225

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPE VRSE+ARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 226 MLFCATREKLDPEVVRSEIARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 285

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 286 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 345

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 346 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 405

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 406 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 465

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 466 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 525

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 526 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 585

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 586 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 645

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 646 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 692

[Close]

BLAST of Lag0023332 vs. NCBI nr
Match: XP_022970665.1 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 621/647 (95.98%), Postives = 634/647 (97.99%), Query Frame = 0


Query: 1    MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
            MASVHA++TPAVGKSGN SYPTKSLN+AFLPGFD+VGR SG CKD Y SSIT+TPRATLT
Sbjct: 591  MASVHATITPAVGKSGNHSYPTKSLNSAFLPGFDMVGRISGACKDSYPSSITITPRATLT 650

Query: 61   FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
             EP ETS  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVH+ETGHVLKVPFRR
Sbjct: 651  SEPMETSRVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 710

Query: 121  VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
            VHLSGDEPNFDNYDTSGPQNISPR GLP+LRKDWVDRRDKLGAPRYTQMYYA+QGIITEE
Sbjct: 711  VHLSGDEPNFDNYDTSGPQNISPRTGLPELRKDWVDRRDKLGAPRYTQMYYARQGIITEE 770

Query: 181  MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
            ML+CATREKLDPE VRSE+ARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 771  MLFCATREKLDPEVVRSEIARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 830

Query: 241  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
            IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 831  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 890

Query: 301  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
            NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 891  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 950

Query: 361  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
            ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 951  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 1010

Query: 421  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
            QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 1011 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 1070

Query: 481  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
            GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 1071 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 1130

Query: 541  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
            WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 1131 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 1190

Query: 601  AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 1191 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 1237

[Close]

BLAST of Lag0023332 vs. ExPASy Swiss-Prot
Match: O82392 (Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THIC PE=1 SV=1)

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 559/646 (86.53%), Postives = 599/646 (92.72%), Query Frame = 0


Query: 2   ASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLTF 61
           ASVH ++   V  + N S   K  N++ LPGFDVV +A+ T         T T RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATLTF 62

Query: 62  EPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRRV 121
           +PP T+ E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of Lag0023332 vs. ExPASy Swiss-Prot
Match: Q9KBJ4 (Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=thiC PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 4.6e-208
Identity = 369/584 (63.18%), Postives = 429/584 (73.46%), Query Frame = 0


Query: 90  SFEQCFPKSTKEHKEVVHEETGHVLKVPFRRVHL------SGDEPN--FDNYDTSGP--- 149
           S +  FP S K +      +    ++VPFR +        SG+  N     YD+SGP   
Sbjct: 12  SIQSSFPNSQKRYVTGSRSD----IRVPFREISQHPTVTDSGEAENEPILVYDSSGPYTD 71

Query: 150 --QNISPRIGLPKLRKDWV----------------------------------------- 209
               ++   GLP +RK W+                                         
Sbjct: 72  ESYEVNIEKGLPAIRKSWILERGDCEKYEGRDVRPEDNGFTKDDDPRAAREVFPRTSSHK 131

Query: 210 DRRDKLGAPRYTQMYYAKQGIITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLEL 269
             R K GA   TQM+YAK+GI+T EM + A RE + PEFVR E+ARGRAI+PSN  H E 
Sbjct: 132 PLRAKKGA-NVTQMHYAKKGIVTPEMEFVAIRENVSPEFVRDELARGRAILPSNINHPES 191

Query: 270 EPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWI 329
           EPMI+GRNF VK+NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI
Sbjct: 192 EPMIIGRNFHVKINANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWI 251

Query: 330 LRNSAVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPL 389
           +RNS VPVGTVPIYQALEKVNG+AE+LTWE++R+TLIEQAEQGVDYFTIHAGVLLRYIPL
Sbjct: 252 IRNSPVPVGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPL 311

Query: 390 TAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYD 449
           TAKR+TGIVSRGGSI A+WCL HHKENF Y H+++I +I   YDI+ S+GDGLRPGSI D
Sbjct: 312 TAKRVTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIAD 371

Query: 450 ANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLG 509
           AND AQFAEL T GELT+ AWE DVQVM EGPGH+PMH+I ENM KQL+ C EAPFYTLG
Sbjct: 372 ANDEAQFAELETLGELTKIAWEHDVQVMVEGPGHVPMHQIKENMDKQLDICQEAPFYTLG 431

Query: 510 PLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHA 569
           PLTTD+APGYDHITSAIGAA IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHA
Sbjct: 432 PLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHA 491

Query: 570 ADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSM 620
           ADLAKGHP AQ  DDALSKARFEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSM
Sbjct: 492 ADLAKGHPGAQKRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSM 551

[Close]

BLAST of Lag0023332 vs. ExPASy Swiss-Prot
Match: A7GUZ5 (Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=thiC PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 1.9e-206
Identity = 366/563 (65.01%), Postives = 422/563 (74.96%), Query Frame = 0


Query: 103 KEVVHEETGHVLKVPFRRVHLSGDE--PN--FDNYDTSGP-----QNISPRIGLPKLRKD 162
           K+V  E +   +KVP R + LS     PN     YDTSGP       +    G+P  R++
Sbjct: 20  KKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRRN 79

Query: 163 WV----------------------------------DR---RDKLGAPRYTQMYYAKQGI 222
           W+                                  DR   R K GA   TQM+YA++GI
Sbjct: 80  WIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGA-NVTQMHYARKGI 139

Query: 223 ITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSA 282
           IT EM Y A RE ++PEFVR E+A GRAI+P+N  H E EPMI+GRNF VKVNANIGNSA
Sbjct: 140 ITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSA 199

Query: 283 VASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVN 342
           V+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI+RN+ VPVGTVPIYQALEKV 
Sbjct: 200 VSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRNAPVPVGTVPIYQALEKVQ 259

Query: 343 GIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL 402
           GIAENLTWE++R+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSI A+WCL
Sbjct: 260 GIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWCL 319

Query: 403 AHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAW 462
            HHKENF Y H+++I +I  QYD+S S+GDGLRPGSI DAND AQFAEL T GELT+ AW
Sbjct: 320 YHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIAW 379

Query: 463 EKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAAN 522
           + DVQVM EGPGH+PMH I ENM+K+++ C  APFYTLGPLTTD+APGYDHITSAIGAA 
Sbjct: 380 KHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAM 439

Query: 523 IGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKAR 582
           IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHAADLAKGH  AQ  DDALSKAR
Sbjct: 440 IGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADLAKGHKTAQQRDDALSKAR 499

Query: 583 FEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEH 620
           FEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSMCGPKFCSMKI+ D+R+YA+E+
Sbjct: 500 FEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKEN 559

[Close]

BLAST of Lag0023332 vs. ExPASy Swiss-Prot
Match: A4VR38 (Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=379731 GN=thiC PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 7.3e-206
Identity = 369/600 (61.50%), Postives = 425/600 (70.83%), Query Frame = 0


Query: 93  QCFPKSTKEHKEVVHEETGHVLKVPFRRVHLSGDEPNFDN--------YDTSGPQ----- 152
           Q FP+S K + +    +    ++VP R + L     +F          YDTSGP      
Sbjct: 21  QPFPRSQKIYVQGSRPD----IRVPMREISLDVTPTDFGGEINAPVTVYDTSGPYTDPNV 80

Query: 153 NISPRIGLPKLRKDWV-DRRD-------------------KLGAPRY------------- 212
            I  R GL  +R  W+ DR D                   +L A R+             
Sbjct: 81  TIDVRKGLADVRSAWIEDRGDTEKLPGLTSEFGQRRLNDAELSAMRFAHVRNPRRAKAEH 140

Query: 213 --TQMYYAKQGIITEEMLYCATRE----------------------------KLDPEFVR 272
             +QM+YAK+GIIT EM Y A RE                            ++ PEFVR
Sbjct: 141 NVSQMHYAKKGIITPEMEYVAIRENMKLAEAREAGLLVEQHAGQSFGAAIPKEITPEFVR 200

Query: 273 SEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGA 332
           SEVARGRAIIP+N  H ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WG+
Sbjct: 201 SEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGS 260

Query: 333 DTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAE 392
           DTVMDLSTG+HIHETREWI+RNS VP+GTVPIYQALEKVNG+AE+LTWE+FR+TLIEQAE
Sbjct: 261 DTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAE 320

Query: 393 QGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICN 452
           QGVDYFTIHAGVLLRY+PLTAKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I  
Sbjct: 321 QGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMK 380

Query: 453 QYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIP 512
            YD+S S+GDGLRPGSI DAND AQF EL T GELT+ AW+ DVQ M EGPGH+PMH I 
Sbjct: 381 AYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQCMIEGPGHVPMHMIK 440

Query: 513 ENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGL 572
           ENM KQLE C+EAPFYTLGPLTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLGL
Sbjct: 441 ENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGL 500

Query: 573 PNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMS 617
           PN+DDVK G+I YKIAAHAADLAKGHP AQ  D+ALSKARFEFRW DQF L LDP TA +
Sbjct: 501 PNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARA 560

[Close]

BLAST of Lag0023332 vs. ExPASy Swiss-Prot
Match: A6VD86 (Phosphomethylpyrimidine synthase OS=Pseudomonas aeruginosa (strain PA7) OX=381754 GN=thiC PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 2.1e-205
Identity = 369/601 (61.40%), Postives = 420/601 (69.88%), Query Frame = 0


Query: 93  QCFPKSTKEHKEVVHEETGHVLKVPFRRVHLSGDEPNFDN--------YDTSGPQ----- 152
           Q FP S K +      +    ++VP R + L+     F          YDTSGP      
Sbjct: 21  QPFPNSRKVYLTGSRPD----IRVPVREISLADTPTAFGGEKNPPVFVYDTSGPYTDPEV 80

Query: 153 NISPRIGLPKLRKDWVD------------------------------------RRDKLGA 212
            I  R GLP +R  W+D                                    RR K GA
Sbjct: 81  RIDLRKGLPDVRSRWIDERGDTEILPGLTSEFGQARLADASLDALRFAHVRTPRRAKPGA 140

Query: 213 PRYTQMYYAKQGIITEEMLYCATRE----------------------------KLDPEFV 272
              +QM+YAK+GIIT EM Y A RE                            ++ PEFV
Sbjct: 141 -NVSQMHYAKKGIITPEMEYIAIRENMKLQEARAAGLLDQQHPGHSFGANIPKEITPEFV 200

Query: 273 RSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWG 332
           R EVARGRAIIP+N  H ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WG
Sbjct: 201 REEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVEKLTWGIRWG 260

Query: 333 ADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQA 392
           ADTVMDLSTG+HIHETREWILRNS VP+GTVPIYQALEKVNG+AE+LTWEIFR+TLIEQA
Sbjct: 261 ADTVMDLSTGKHIHETREWILRNSPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQA 320

Query: 393 EQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDIC 452
           EQGVDYFTIHAGVLLRY+PLTAKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I 
Sbjct: 321 EQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIM 380

Query: 453 NQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKI 512
             YD+S S+GDGLRPGS+ DAND AQF EL T GELT+ AW+ DVQVM EGPGH+PM  I
Sbjct: 381 KAYDVSFSLGDGLRPGSVADANDAAQFGELETLGELTKIAWKHDVQVMIEGPGHVPMQLI 440

Query: 513 PENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLG 572
            ENM KQLE C+EAPFYTLGPLTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLG
Sbjct: 441 KENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLG 500

Query: 573 LPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAM 617
           LPN+DDVK G+I YKIAAHAADLAKGHP AQ  D+ALSKARFEFRW DQF L LDP TA 
Sbjct: 501 LPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTAR 560

[Close]

BLAST of Lag0023332 vs. ExPASy TrEMBL
Match: A0A6J1HP78 (phosphomethylpyrimidine synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464788 PE=3 SV=1)

HSP 1 Score: 1297.3 bits (3356), Expect = 0.0e+00
Identity = 624/647 (96.45%), Postives = 633/647 (97.84%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGKSGN SYPTKSLNTAFLPGFDVVGR SG CK+ Y SSITLTPRATLT
Sbjct: 1   MASVHATITPAVGKSGNHSYPTKSLNTAFLPGFDVVGRVSGACKNSYPSSITLTPRATLT 60

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
            EP E S  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR
Sbjct: 61  SEPMENSTVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR GLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRTGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVP+YQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPVYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           Q+MNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QIMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 601 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 647

[Close]

BLAST of Lag0023332 vs. ExPASy TrEMBL
Match: A0A6J1I4J9 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469580 PE=3 SV=1)

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 621/647 (95.98%), Postives = 634/647 (97.99%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGKSGN SYPTKSLN+AFLPGFD+VGR SG CKD Y SSIT+TPRATLT
Sbjct: 46  MASVHATITPAVGKSGNHSYPTKSLNSAFLPGFDMVGRISGACKDSYPSSITITPRATLT 105

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
            EP ETS  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVH+ETGHVLKVPFRR
Sbjct: 106 SEPMETSRVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 165

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR GLP+LRKDWVDRRDKLGAPRYTQMYYA+QGIITEE
Sbjct: 166 VHLSGDEPNFDNYDTSGPQNISPRTGLPELRKDWVDRRDKLGAPRYTQMYYARQGIITEE 225

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPE VRSE+ARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 226 MLFCATREKLDPEVVRSEIARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 285

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 286 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 345

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 346 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 405

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 406 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 465

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 466 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 525

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 526 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 585

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 586 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 645

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 646 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 692

[Close]

BLAST of Lag0023332 vs. ExPASy TrEMBL
Match: A0A6J1I3H6 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111469580 PE=3 SV=1)

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 621/647 (95.98%), Postives = 634/647 (97.99%), Query Frame = 0


Query: 1    MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
            MASVHA++TPAVGKSGN SYPTKSLN+AFLPGFD+VGR SG CKD Y SSIT+TPRATLT
Sbjct: 591  MASVHATITPAVGKSGNHSYPTKSLNSAFLPGFDMVGRISGACKDSYPSSITITPRATLT 650

Query: 61   FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
             EP ETS  KAK  KHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVH+ETGHVLKVPFRR
Sbjct: 651  SEPMETSRVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 710

Query: 121  VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
            VHLSGDEPNFDNYDTSGPQNISPR GLP+LRKDWVDRRDKLGAPRYTQMYYA+QGIITEE
Sbjct: 711  VHLSGDEPNFDNYDTSGPQNISPRTGLPELRKDWVDRRDKLGAPRYTQMYYARQGIITEE 770

Query: 181  MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
            ML+CATREKLDPE VRSE+ARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 771  MLFCATREKLDPEVVRSEIARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 830

Query: 241  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
            IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 831  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 890

Query: 301  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
            NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 891  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 950

Query: 361  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
            ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 951  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 1010

Query: 421  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
            QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 1011 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 1070

Query: 481  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
            GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 1071 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 1130

Query: 541  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
            WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 1131 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 1190

Query: 601  AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            AEEALKQGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 1191 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 1237

[Close]

BLAST of Lag0023332 vs. ExPASy TrEMBL
Match: A0A1S3C5I7 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497093 PE=3 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 622/647 (96.14%), Postives = 635/647 (98.15%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGKS NRS PTKSLNTAFLPGFDVVGR + TCKDL+ SSITL PRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 60

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
           F+PPETS EKAK  KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDWVDRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASY++S KR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of Lag0023332 vs. ExPASy TrEMBL
Match: A0A5D3BDX2 (Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001330 PE=3 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 622/647 (96.14%), Postives = 635/647 (98.15%), Query Frame = 0


Query: 1   MASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLT 60
           MASVHA++TPAVGKS NRS PTKSLNTAFLPGFDVVGR + TCKDL+ SSITL PRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 60

Query: 61  FEPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120
           F+PPETS EKAK  KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDWVDRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           ML+CATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASY++S KR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of Lag0023332 vs. TAIR 10
Match: AT2G29630.2 (thiaminC )

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 559/646 (86.53%), Postives = 599/646 (92.72%), Query Frame = 0


Query: 2   ASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLTF 61
           ASVH ++   V  + N S   K  N++ LPGFDVV +A+ T         T T RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATLTF 62

Query: 62  EPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRRV 121
           +PP T+ E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of Lag0023332 vs. TAIR 10
Match: AT2G29630.1 (thiaminC )

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 559/646 (86.53%), Postives = 599/646 (92.72%), Query Frame = 0


Query: 2   ASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLTF 61
           ASVH ++   V  + N S   K  N++ LPGFDVV +A+ T         T T RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATLTF 62

Query: 62  EPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRRV 121
           +PP T+ E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of Lag0023332 vs. TAIR 10
Match: AT2G29630.3 (thiaminC )

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 559/646 (86.53%), Postives = 599/646 (92.72%), Query Frame = 0


Query: 2   ASVHASVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRASGTCKDLYSSSITLTPRATLTF 61
           ASVH ++   V  + N S   K  N++ LPGFDVV +A+ T         T T RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATLTF 62

Query: 62  EPPETSMEKAKTKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRRV 121
           +PP T+ E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

The following BLAST results are available for this feature:

BLAST of Lag0023332 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519726.1	0.0e+00	97.06	phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita pepo ...	[more]
KAG6583379.1	0.0e+00	96.91	Phosphomethylpyrimidine synthase, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_022964789.1	0.0e+00	96.45	phosphomethylpyrimidine synthase, chloroplastic [Cucurbita moschata]	[more]
XP_022970663.1	0.0e+00	95.98	phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita maxim...	[more]
XP_022970665.1	0.0e+00	95.98	phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 [Cucurbita maxim...	[more]

BLAST of Lag0023332 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82392	0.0e+00	86.53	Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9KBJ4	4.6e-208	63.18	Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / D...	[more]
A7GUZ5	1.9e-206	65.01	Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP...	[more]
A4VR38	7.3e-206	61.50	Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=37973...	[more]
A6VD86	2.1e-205	61.40	Phosphomethylpyrimidine synthase OS=Pseudomonas aeruginosa (strain PA7) OX=38175...	[more]

BLAST of Lag0023332 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HP78	0.0e+00	96.45	phosphomethylpyrimidine synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1I4J9	0.0e+00	95.98	phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 OS=Cucurbita max...	[more]
A0A6J1I3H6	0.0e+00	95.98	phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 OS=Cucurbita max...	[more]
A0A1S3C5I7	0.0e+00	96.14	phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucumis melo OX=36...	[more]
A0A5D3BDX2	0.0e+00	96.14	Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Lag0023332 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29630.2	0.0e+00	86.53	thiaminC	[more]
AT2G29630.1	0.0e+00	86.53	thiaminC	[more]
AT2G29630.3	0.0e+00	86.53	thiaminC	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038521	ThiC/Bza, core domain	GENE3D	3.20.20.540	Radical SAM ThiC family, central domain	coord: 209..523 e-value: 1.3E-168 score: 561.6
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	PFAM	PF01964	ThiC_Rad_SAM	coord: 167..587 e-value: 1.6E-197 score: 656.4
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	TIGRFAM	TIGR00190	TIGR00190	coord: 167..590 e-value: 6.9E-204 score: 675.3
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	SFLD	SFLDF00407	phosphomethylpyrimidine_synth	coord: 167..587 e-value: 0.0 score: 657.6
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	PANTHER	PTHR30557	THIAMINE BIOSYNTHESIS PROTEIN THIC	coord: 1..647
None	No IPR available	GENE3D	6.10.250.620		coord: 527..559 e-value: 3.9E-18 score: 67.0
None	No IPR available	SFLD	SFLDS00113	Radical_SAM_Phosphomethylpyri	coord: 165..623 e-value: 0.0 score: 857.3
None	No IPR available	SFLD	SFLDG01114	phosphomethylpyrimidine_synth	coord: 165..621 e-value: 0.0 score: 838.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR30557:SF2	SUBFAMILY NOT NAMED	coord: 1..647
IPR037509	Phosphomethylpyrimidine synthase	HAMAP	MF_00089	ThiC	coord: 166..592 score: 94.826241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0023332.1	Lag0023332.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009228	thiamine biosynthetic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009526	plastid envelope
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0016830	carbon-carbon lyase activity
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding