Lag0023261 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0023261 vs. NCBI nr
Match: XP_038893602.1 (uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893603.1 uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893604.1 uncharacterized protein LOC120082486 [Benincasa hispida])

HSP 1 Score: 539.3 bits (1388), Expect = 2.5e-149
Identity = 260/281 (92.53%), Postives = 273/281 (97.15%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI 123
           MENGDQF Q SDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMI+KLGMEENVPELCI
Sbjct: 1   MENGDQFNQISDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIEKLGMEENVPELCI 60

Query: 124 SLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTN 183
           SLYKIYGTT+AGLRAIGYNFDYDDFHSFVHGRLPYDMLK DPVLRNLLHSLPIRKLIFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPVLRNLLHSLPIRKLIFTN 120

Query: 184 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDL 243
           GDMAHA RALKRLGLEDCFEGILCFETLNPDKGT VD+EAGS IF+INQYM+NPNSD+DL
Sbjct: 121 GDMAHATRALKRLGLEDCFEGILCFETLNPDKGT-VDEEAGSVIFNINQYMSNPNSDIDL 180

Query: 244 PKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGV 303
           PKTPVVCKPFEEAFKQVFE+ANINP+KTLFFDDSVRNLQTGK VGLHTVLVGNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEIANINPQKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240

Query: 304 DHAFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           DHAFESIHNI+EGLPELWEA+EKL +++YSEKVAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEAMEKLNSISYSEKVAIETSVRA 280

BLAST of Lag0023261 vs. NCBI nr
Match: XP_016902092.1 (PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_016902093.1 PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_016902094.1 PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo])

HSP 1 Score: 536.6 bits (1381), Expect = 1.6e-148
Identity = 256/281 (91.10%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI 123
           MENGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 124 SLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTN 183
           SLYKIYGTT+AGL+AIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRKLIFTN
Sbjct: 61  SLYKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTN 120

Query: 184 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDL 243
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTV ++EAGS IFDINQYM+NPNSD+DL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDL 180

Query: 244 PKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGV 303
           PKTPVVCKPFEEAFKQVFEMANINP+KTLFFDDSVRNLQTGK VGLHTVL+GNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGV 240

Query: 304 DHAFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           DHAFESIHNI+EGLPELWE +EKL++V YS KVAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKVAIETSVRA 281

BLAST of Lag0023261 vs. NCBI nr
Match: KAA0067755.1 (Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 532.7 bits (1371), Expect = 2.3e-147
Identity = 254/279 (91.04%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 66  NGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCISL 125
           NGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCISL
Sbjct: 280 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 339

Query: 126 YKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGD 185
           YKIYGTT+AGL+AIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRKLIFTNGD
Sbjct: 340 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 399

Query: 186 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPK 245
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTV ++EAGS IFDINQYM+NPNSD+DLPK
Sbjct: 400 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 459

Query: 246 TPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDH 305
           TPVVCKPFEEAFKQVFEMANINP+KTLFFDDSVRNLQTGK VGLHTVL+GNSQRIKGVDH
Sbjct: 460 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 519

Query: 306 AFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           AFESIHNI+EGLPELWE +EKL++V YS KVAIETSVRA
Sbjct: 520 AFESIHNIKEGLPELWEDMEKLKSVTYSRKVAIETSVRA 558

BLAST of Lag0023261 vs. NCBI nr
Match: TYJ97389.1 (Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 532.7 bits (1371), Expect = 2.3e-147
Identity = 254/279 (91.04%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 66  NGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCISL 125
           NGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCISL
Sbjct: 199 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 258

Query: 126 YKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGD 185
           YKIYGTT+AGL+AIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRKLIFTNGD
Sbjct: 259 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 318

Query: 186 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPK 245
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTV ++EAGS IFDINQYM+NPNSD+DLPK
Sbjct: 319 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 378

Query: 246 TPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDH 305
           TPVVCKPFEEAFKQVFEMANINP+KTLFFDDSVRNLQTGK VGLHTVL+GNSQRIKGVDH
Sbjct: 379 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 438

Query: 306 AFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           AFESIHNI+EGLPELWE +EKL++V YS KVAIETSVRA
Sbjct: 439 AFESIHNIKEGLPELWEDMEKLKSVTYSRKVAIETSVRA 477

BLAST of Lag0023261 vs. NCBI nr
Match: XP_011658227.1 (uncharacterized protein C24B11.05 [Cucumis sativus] >XP_011658231.1 uncharacterized protein C24B11.05 [Cucumis sativus] >KGN65738.1 hypothetical protein Csa_019919 [Cucumis sativus])

HSP 1 Score: 526.2 bits (1354), Expect = 2.2e-145
Identity = 256/282 (90.78%), Postives = 269/282 (95.39%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI 123
           MENGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 124 SLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTN 183
           SLYKIYGTT+AGLRAIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120

Query: 184 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDL 243
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGT VD+E GS IFDINQYM+NPNSDLDL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGT-VDEEEGSVIFDINQYMSNPNSDLDL 180

Query: 244 PKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGV 303
           PKTPVVCKPFEEA+KQVFE+ANINP+KTLFFDDSVRNLQTGK VGLHTVLVGNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240

Query: 304 DHAFESIHNIREGLPELWEAVEKLENVAYSEK-VAIETSVRA 345
           DHAFESIHNI+EGLPELWE +EKL++V YS K VAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281

BLAST of Lag0023261 vs. ExPASy Swiss-Prot
Match: Q09893 (Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24B11.05 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-15
Identity = 75/252 (29.76%), Postives = 112/252 (44.44%), Query Frame = 0

Query: 81  LLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGM-EENVPELCISLYKIYGTTLAGLRAI 140
           + FDLD+ LYP +  + N +   I  +   KLG+  E    L    Y+ YG  + GL  +
Sbjct: 8   IFFDLDNCLYPKSYKIHNMMAARITAFFSDKLGIPTEEAERLREVYYRHYGIAIRGL-VL 67

Query: 141 GYNFDYDDFHSFVHGRLPYD-MLKADPVLRNLLHSLPIRK----LIFTNGDMAHANRALK 200
            +  D  D+   V   LP + ++K D VLR +L  L +RK     IFTN  + HANR LK
Sbjct: 68  HHEIDAVDYDQRVDQSLPLEKVIKKDEVLREML--LELRKKYKCWIFTNAYIVHANRVLK 127

Query: 201 RLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPKTPVVCKPFE 260
            LG+EDCF+GI                           Y +    DL       + KP  
Sbjct: 128 YLGIEDCFDGI--------------------------TYCDYNAKDL-------IAKPMP 187

Query: 261 EAFKQVFEMANI-NPEKTLFFDDSVRNLQTGKSVG-LHTV-LVGNSQRI---KGVDHAFE 320
           E +++V   A + + +K +F DDS  N+   +  G  +TV LV +   +   +   H   
Sbjct: 188 EMYERVMREAGVTDKDKCIFVDDSYGNILGAREFGWKYTVQLVEHGDPLPQPQAGSHVIR 223

BLAST of Lag0023261 vs. ExPASy Swiss-Prot
Match: P53078 (Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SDT1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 9.5e-11
Identity = 61/219 (27.85%), Postives = 102/219 (46.58%), Query Frame = 0

Query: 83  FDLDDTLYPFNSGLANEITKNIQEYMIKKLGME-ENVPELCISLYKIYGTTLAGLRAIGY 142
           FD+D+ LY  ++ + + + ++I  +    L +  E+   L  S YK YG  + GL  + +
Sbjct: 60  FDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYYKEYGLAIRGL-VMFH 119

Query: 143 NFDYDDFHSFVHGRLP-YDMLKADPVLRNLL----HSLPIRKL-IFTNGDMAHANRALKR 202
             +  +++  V   LP  D+LK D  LRN+L     S  I KL +FTN    HA R L+ 
Sbjct: 120 KVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWLFTNAYKNHAIRCLRL 179

Query: 203 LGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPKTPVVCKPFEE 262
           LG+ D F+G+                           Y +   +D       +VCKP  +
Sbjct: 180 LGIADLFDGL--------------------------TYCDYSRTD------TLVCKPHVK 239

Query: 263 AFKQVFEMANI-NPEKTLFFDDSVRNLQTGKSVGLHTVL 294
           AF++  + + +   E   F DDS +N++TG  +G+ T +
Sbjct: 240 AFEKAMKESGLARYENAYFIDDSGKNIETGIKLGMKTCI 245

BLAST of Lag0023261 vs. ExPASy Swiss-Prot
Match: P40025 (Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PHM8 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.1e-09
Identity = 45/140 (32.14%), Postives = 73/140 (52.14%), Query Frame = 0

Query: 75  DAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI-SLYKIYGTTL 134
           D   +   FD+D+TLY  ++ +   + +++  +   +LG +++  E  I S Y+ YG ++
Sbjct: 49  DPARKVFFFDIDNTLYRKSTKVQLLMQQSLSNFFKYELGFDDDEAERLIESYYQEYGLSV 108

Query: 135 AGLRAIGYNFDYDDFHSFVHGRLP-YDMLKADPVLRNLLHSLPIRKL-------IFTNGD 194
            GL       D   +++F+   LP  D LK D  LR LL +L  +KL       +FTN  
Sbjct: 109 KGLIKNKQIDDVLQYNTFIDDSLPLQDYLKPDWKLRELLINLKKKKLGKFDKLWLFTNSY 168

Query: 195 MAHANRALKRLGLEDCFEGI 206
             HA R +K LG+ D F+GI
Sbjct: 169 KNHAIRCVKILGIADLFDGI 188

BLAST of Lag0023261 vs. ExPASy TrEMBL
Match: A0A1S4E1J3 (uncharacterized protein C24B11.05-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497146 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.8e-149
Identity = 256/281 (91.10%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI 123
           MENGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 124 SLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTN 183
           SLYKIYGTT+AGL+AIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRKLIFTN
Sbjct: 61  SLYKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTN 120

Query: 184 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDL 243
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTV ++EAGS IFDINQYM+NPNSD+DL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDL 180

Query: 244 PKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGV 303
           PKTPVVCKPFEEAFKQVFEMANINP+KTLFFDDSVRNLQTGK VGLHTVL+GNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGV 240

Query: 304 DHAFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           DHAFESIHNI+EGLPELWE +EKL++V YS KVAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKVAIETSVRA 281

BLAST of Lag0023261 vs. ExPASy TrEMBL
Match: A0A5D3BED8 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002080 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.1e-147
Identity = 254/279 (91.04%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 66  NGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCISL 125
           NGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCISL
Sbjct: 199 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 258

Query: 126 YKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGD 185
           YKIYGTT+AGL+AIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRKLIFTNGD
Sbjct: 259 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 318

Query: 186 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPK 245
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTV ++EAGS IFDINQYM+NPNSD+DLPK
Sbjct: 319 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 378

Query: 246 TPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDH 305
           TPVVCKPFEEAFKQVFEMANINP+KTLFFDDSVRNLQTGK VGLHTVL+GNSQRIKGVDH
Sbjct: 379 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 438

Query: 306 AFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           AFESIHNI+EGLPELWE +EKL++V YS KVAIETSVRA
Sbjct: 439 AFESIHNIKEGLPELWEDMEKLKSVTYSRKVAIETSVRA 477

BLAST of Lag0023261 vs. ExPASy TrEMBL
Match: A0A5A7VPY6 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00750 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.1e-147
Identity = 254/279 (91.04%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 66  NGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCISL 125
           NGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCISL
Sbjct: 280 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 339

Query: 126 YKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGD 185
           YKIYGTT+AGL+AIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRKLIFTNGD
Sbjct: 340 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 399

Query: 186 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPK 245
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTV ++EAGS IFDINQYM+NPNSD+DLPK
Sbjct: 400 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 459

Query: 246 TPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDH 305
           TPVVCKPFEEAFKQVFEMANINP+KTLFFDDSVRNLQTGK VGLHTVL+GNSQRIKGVDH
Sbjct: 460 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 519

Query: 306 AFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           AFESIHNI+EGLPELWE +EKL++V YS KVAIETSVRA
Sbjct: 520 AFESIHNIKEGLPELWEDMEKLKSVTYSRKVAIETSVRA 558

BLAST of Lag0023261 vs. ExPASy TrEMBL
Match: A0A0A0LVD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523620 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.0e-145
Identity = 256/282 (90.78%), Postives = 269/282 (95.39%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI 123
           MENGD+F Q SDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMI+KLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 124 SLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTN 183
           SLYKIYGTT+AGLRAIGYNFDYDDFHSFVHGRLPYDMLK DP+LRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120

Query: 184 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDL 243
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGT VD+E GS IFDINQYM+NPNSDLDL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGT-VDEEEGSVIFDINQYMSNPNSDLDL 180

Query: 244 PKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGV 303
           PKTPVVCKPFEEA+KQVFE+ANINP+KTLFFDDSVRNLQTGK VGLHTVLVGNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240

Query: 304 DHAFESIHNIREGLPELWEAVEKLENVAYSEK-VAIETSVRA 345
           DHAFESIHNI+EGLPELWE +EKL++V YS K VAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281

BLAST of Lag0023261 vs. ExPASy TrEMBL
Match: A0A6J1HLM8 (uncharacterized protein LOC111464700 OS=Cucurbita moschata OX=3662 GN=LOC111464700 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 3.0e-140
Identity = 238/281 (84.70%), Postives = 266/281 (94.66%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEENVPELCI 123
           MENGDQF QFSDAKYECLLFDLDDTLYP NSGL+ E++KNIQE+MI+KLG+EENVPELCI
Sbjct: 1   MENGDQFTQFSDAKYECLLFDLDDTLYPLNSGLSKEVSKNIQEFMIEKLGIEENVPELCI 60

Query: 124 SLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTN 183
           SLYKIYGTTLAGLRAIGY+FDYDDFHSF HGRLPYD LK DPVLRNLLHSLPIRKL+FTN
Sbjct: 61  SLYKIYGTTLAGLRAIGYDFDYDDFHSFAHGRLPYDRLKPDPVLRNLLHSLPIRKLLFTN 120

Query: 184 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDL 243
           GDMAHA RAL+RLGLEDCFEGILCFETLNP K TVVD+ AGS +FDI+Q+M++ NSD+DL
Sbjct: 121 GDMAHATRALQRLGLEDCFEGILCFETLNPSKETVVDEAAGSVVFDIDQFMSSLNSDIDL 180

Query: 244 PKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGV 303
           PKTP++CKPFEEA+KQVFE+ANINP+KTLFFDDSVRNLQ GKSVGLHTVL+G+SQ+I GV
Sbjct: 181 PKTPIICKPFEEAYKQVFEIANINPQKTLFFDDSVRNLQAGKSVGLHTVLIGSSQQINGV 240

Query: 304 DHAFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
           DHAFE+IHNIREGLPELW+ +EKLENV+YSEKVAIETSVRA
Sbjct: 241 DHAFENIHNIREGLPELWDTMEKLENVSYSEKVAIETSVRA 281

BLAST of Lag0023261 vs. TAIR 10
Match: AT5G59480.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.0e-104
Identity = 184/276 (66.67%), Postives = 223/276 (80.80%), Query Frame = 0

Query: 68  DQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEEN-VPELCISLY 127
           + F+Q S+AKY+CLLFD+DDTLYP +SGLA E+ KNIQEYM++KLG+EE+ V ELC+SLY
Sbjct: 5   EHFQQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLY 64

Query: 128 KIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGDM 187
           KIYGTT+AGL+A+GY+FDYDDFH FVHGRLPY  LK DP+LRN++ SLPIRK++FTN D 
Sbjct: 65  KIYGTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRKVVFTNADK 124

Query: 188 AHANRALKRLGLEDCFEGILCFETLNP-DKGTVVDDEAGSEIFDINQYMNNPNSDLDLPK 247
           AHA + + RLGLE CFE I+ FETLNP  K     D    EIFDI  YM NP+S ++LPK
Sbjct: 125 AHAAKIIARLGLEGCFEKIISFETLNPITKTESPVDTKTREIFDIISYMANPDSSIELPK 184

Query: 248 TPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDH 307
           T VVCKP E AF+QVF+MANINP+KTLFFDDS+RN+QTGK VGLHTV VG S R +GVD 
Sbjct: 185 TSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHREEGVDI 244

Query: 308 AFESIHNIREGLPELWEAV-EKLENVAYSEKVAIET 341
           A E IHNIRE LP+LW+AV +K + +   +KVAIET
Sbjct: 245 ALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 280

BLAST of Lag0023261 vs. TAIR 10
Match: AT5G59480.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 6.4e-103
Identity = 184/276 (66.67%), Postives = 222/276 (80.43%), Query Frame = 0

Query: 68  DQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEEN-VPELCISLY 127
           + F+Q S+AKY+CLLFD+DDTLYP +SGLA E+ KNIQEYM++KLG+EE+ V ELC+SLY
Sbjct: 5   EHFQQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLY 64

Query: 128 KIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGDM 187
           KIYGTT+AGL+A+GY+FDYDDFH FVHGRLPY  LK DP+LRN++ SLPIRK +FTN D 
Sbjct: 65  KIYGTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRK-VFTNADK 124

Query: 188 AHANRALKRLGLEDCFEGILCFETLNP-DKGTVVDDEAGSEIFDINQYMNNPNSDLDLPK 247
           AHA + + RLGLE CFE I+ FETLNP  K     D    EIFDI  YM NP+S ++LPK
Sbjct: 125 AHAAKIIARLGLEGCFEKIISFETLNPITKTESPVDTKTREIFDIISYMANPDSSIELPK 184

Query: 248 TPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDH 307
           T VVCKP E AF+QVF+MANINP+KTLFFDDS+RN+QTGK VGLHTV VG S R +GVD 
Sbjct: 185 TSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHREEGVDI 244

Query: 308 AFESIHNIREGLPELWEAV-EKLENVAYSEKVAIET 341
           A E IHNIRE LP+LW+AV +K + +   +KVAIET
Sbjct: 245 ALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 279

BLAST of Lag0023261 vs. TAIR 10
Match: AT5G02230.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.4e-94
Identity = 169/282 (59.93%), Postives = 213/282 (75.53%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGM-EENVPELC 123
           ME  +++   +  KY+CLLFDLDDTLYP +SG+A E   NI++YM +KLG+ ++ + EL 
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 124 ISLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFT 183
             LYK YGTT+AGLRAIGY FDYD++HSFVHGRLPYD +K D VLR+LL SLP+RK+IFT
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 184 NGDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLD 243
           N D  HA +ALK+LGLEDCFEGI+CFETLN        +   SEIFDI  + N       
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNLMHTNAASN--NSEIFDIVGHFNRSEPVGS 180

Query: 244 LPKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKG 303
           LPKTPVVCKP E A ++  E+ANI+P +TLFF+DSVRN+Q GK VGL+TVLVG S ++KG
Sbjct: 181 LPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKG 240

Query: 304 VDHAFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
            D+A E+IHN++E +PELWE+  K  +V YS KVA+ETSVRA
Sbjct: 241 ADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSVRA 280

BLAST of Lag0023261 vs. TAIR 10
Match: AT5G02230.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.4e-94
Identity = 169/282 (59.93%), Postives = 213/282 (75.53%), Query Frame = 0

Query: 64  MENGDQFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGM-EENVPELC 123
           ME  +++   +  KY+CLLFDLDDTLYP +SG+A E   NI++YM +KLG+ ++ + EL 
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 124 ISLYKIYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFT 183
             LYK YGTT+AGLRAIGY FDYD++HSFVHGRLPYD +K D VLR+LL SLP+RK+IFT
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 184 NGDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLD 243
           N D  HA +ALK+LGLEDCFEGI+CFETLN        +   SEIFDI  + N       
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNLMHTNAASN--NSEIFDIVGHFNRSEPVGS 180

Query: 244 LPKTPVVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKG 303
           LPKTPVVCKP E A ++  E+ANI+P +TLFF+DSVRN+Q GK VGL+TVLVG S ++KG
Sbjct: 181 LPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKG 240

Query: 304 VDHAFESIHNIREGLPELWEAVEKLENVAYSEKVAIETSVRA 345
            D+A E+IHN++E +PELWE+  K  +V YS KVA+ETSVRA
Sbjct: 241 ADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSVRA 280

BLAST of Lag0023261 vs. TAIR 10
Match: AT5G59490.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 5.1e-84
Identity = 153/281 (54.45%), Postives = 207/281 (73.67%), Query Frame = 0

Query: 69  QFKQFSDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIKKLGMEEN-VPELCISLYK 128
           +F   S  +YECLLFDLDDTLYP +SGL++  + NI EYM++KLG++E+ V EL   LYK
Sbjct: 2   EFVNSSPPRYECLLFDLDDTLYPLSSGLSDACSNNIIEYMVEKLGIDEDGVVELNQILYK 61

Query: 129 IYGTTLAGLRAIGYNFDYDDFHSFVHGRLPYDMLKADPVLRNLLHSLPIRKLIFTNGDMA 188
            YGT++AGL+A+GY FD D++H +VHGRLPY+ LK DPVLR+LL  LP+RKL+F+NGD  
Sbjct: 62  KYGTSMAGLKAVGYEFDNDEYHRYVHGRLPYENLKPDPVLRSLLLGLPLRKLVFSNGDEV 121

Query: 189 HANRALKRLGLEDCFEGILCFETLNPDKGTVVDDEAGSEIFDINQYMNNPNSDLDLPKTP 248
           H  +AL RLG+EDCFE I+ FETLNPD          +E+  +  +         LP+ P
Sbjct: 122 HVMKALTRLGIEDCFERIISFETLNPDINE-------AELSCVTGH---------LPENP 181

Query: 249 VVCKPFEEAFKQVFEMANINPEKTLFFDDSVRNLQTGKSVGLHTVLVGNSQRIKGVDHAF 308
           V+CKP E AF++ F++A +NP KTLFFDDS RN+QTGK+VGLHTVLVG S++I G D+A 
Sbjct: 182 VICKPTEIAFEKAFDIAQLNPHKTLFFDDSTRNIQTGKAVGLHTVLVGKSEKIDGSDYAL 241

Query: 309 ESIHNIREGLPELW-EAV---EKLENVAYSEKVAIETSVRA 345
           ESIHN++E  PELW E++   ++ E + Y+ +++IETSV+A
Sbjct: 242 ESIHNMKEAFPELWSESIINNKETERIDYASQISIETSVQA 266