Lag0023060 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0023060 vs. NCBI nr
Match: XP_038893965.1 (uncharacterized protein LOC120082751 [Benincasa hispida])

HSP 1 Score: 967.6 bits (2500), Expect = 4.5e-278
Identity = 471/551 (85.48%), Postives = 502/551 (91.11%), Query Frame = 0

Query: 1   MADTMDDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADTMDDD +QD+IT+QRSELL+AKTL SDLDFAFQLQLQEAMNASL S PS EPS SLN
Sbjct: 1   MADTMDDDSIQDLITQQRSELLSAKTLFSDLDFAFQLQLQEAMNASLASKPSTEPSSSLN 60

Query: 61  RASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
            A PVL+SEETG LDLATTLMLEDIARFAMEFKDRE+C  EMRKMKEELDRR+HDQ FA 
Sbjct: 61  TAVPVLDSEETGALDLATTLMLEDIARFAMEFKDREQCHNEMRKMKEELDRRVHDQKFAE 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEERIR 180
           YI NVPE+EWRKYGDNYEKPYGESSSSSSS  S SS    +DS+CFRVYSKGLISEERIR
Sbjct: 121 YIRNVPENEWRKYGDNYEKPYGESSSSSSSCSSPSS----VDSECFRVYSKGLISEERIR 180

Query: 181 DMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGLT 240
           DM VRVAGIGVA+CDPKDNLL EK  PLE +VD KE SS+CAELEALVEGLN+AL+LGL 
Sbjct: 181 DMVVRVAGIGVAVCDPKDNLLFEKTSPLESMVDSKETSSQCAELEALVEGLNVALILGLR 240

Query: 241 NVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRLA 300
           NVTFFC DYMLYQY+TGRV PGASS+A LVNEVVLLQGKFT CNPSLVTRNDIKFAFRLA
Sbjct: 241 NVTFFCGDYMLYQYLTGRVLPGASSMAKLVNEVVLLQGKFTYCNPSLVTRNDIKFAFRLA 300

Query: 301 REAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLL 360
           REAIVSQITWPAEA+NGKCLKETCTICFEDVR DQMF +DGCLHRYCFSCMKQHVEVRLL
Sbjct: 301 REAIVSQITWPAEAENGKCLKETCTICFEDVRVDQMFLVDGCLHRYCFSCMKQHVEVRLL 360

Query: 361 NGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSAL 420
           NG+GM  NCPHQGC SEINIESCGKFLE KV EIMSQRIKEASVP+LEKVYCPYS+CS L
Sbjct: 361 NGNGMQANCPHQGCASEINIESCGKFLEPKVFEIMSQRIKEASVPLLEKVYCPYSRCSEL 420

Query: 421 MSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDE 480
           MSK+ELL+YT+ SYIDA+RSGARKCMKCN FFCINCKV WHYNLTCYD+RKLNPN R DE
Sbjct: 421 MSKTELLRYTEDSYIDAKRSGARKCMKCNRFFCINCKVAWHYNLTCYDFRKLNPNLRSDE 480

Query: 481 EMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCPIWD 540
           +MLKSLAS+KLWRQC+ CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TCNCPIWD
Sbjct: 481 KMLKSLASQKLWRQCVMCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCNCPIWD 540

Query: 541 ERNIIRDQRRR 552
           ERNIIR+QRRR
Sbjct: 541 ERNIIRNQRRR 547

BLAST of Lag0023060 vs. NCBI nr
Match: XP_022157931.1 (uncharacterized protein LOC111024540 [Momordica charantia])

HSP 1 Score: 964.9 bits (2493), Expect = 2.9e-277
Identity = 474/554 (85.56%), Postives = 505/554 (91.16%), Query Frame = 0

Query: 1   MADTM-DDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADTM DDD LQ++++EQRSELLAAKTL SDLD AFQLQLQEAMNASL S PS +PSPS 
Sbjct: 1   MADTMDDDDYLQEVVSEQRSELLAAKTLFSDLDLAFQLQLQEAMNASLDSKPSTQPSPSR 60

Query: 61  NRASPVLESEE-TGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNF 120
           NR  P LESE+ +  LDLATTLMLEDIARFAMEFKDRE+C TEMRKMKEELDRRIHDQNF
Sbjct: 61  NRVVPKLESEDSSSALDLATTLMLEDIARFAMEFKDREQCHTEMRKMKEELDRRIHDQNF 120

Query: 121 ANYILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEER 180
           A YI NVPEDEWR+YGDNYEKPY  SS SSSSS SSS +R  LDS+ FRVYSKGLISEER
Sbjct: 121 ATYIRNVPEDEWRRYGDNYEKPYSGSSGSSSSSSSSSPARVCLDSEIFRVYSKGLISEER 180

Query: 181 IRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLG 240
           IRDM+VRVA IGVA+CDPKDNLL EKM PLE VVDGKEI+SECAELEALVEGLN+AL+LG
Sbjct: 181 IRDMKVRVAAIGVAVCDPKDNLLFEKMMPLESVVDGKEINSECAELEALVEGLNVALILG 240

Query: 241 LTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFR 300
           L NVTFFCADYMLYQY+TGRVPPGASSLATLVNEV+LLQ +FT CNPSLVTRND+KFAF+
Sbjct: 241 LKNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVILLQRRFTYCNPSLVTRNDMKFAFK 300

Query: 301 LAREAIVSQITWPAEADNGKCL-KETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEV 360
           LAREAIVSQITWP EA NGKCL KETC ICFEDVR DQMFS+DGCLHRYCFSCMKQHVEV
Sbjct: 301 LAREAIVSQITWPTEAGNGKCLKKETCIICFEDVRVDQMFSVDGCLHRYCFSCMKQHVEV 360

Query: 361 RLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKC 420
           RLLNG+GM VNCPHQGC S INIESCGKFLE KVIEIMSQRIKEASVP+LEKVYCPYS+C
Sbjct: 361 RLLNGNGMKVNCPHQGCTSGINIESCGKFLEPKVIEIMSQRIKEASVPILEKVYCPYSRC 420

Query: 421 SALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPR 480
           SALMSKSELLKYT+AS+I AE+SGARKCMKCN FFCINCKVPWHYNLTCYDYRK NPNPR
Sbjct: 421 SALMSKSELLKYTEASFIGAEQSGARKCMKCNQFFCINCKVPWHYNLTCYDYRKSNPNPR 480

Query: 481 PDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCP 540
           PDE MLKSLASRKLWRQC+KCNNMVELAEGCYHITCRCGYEFCY CGAPWK KK TC CP
Sbjct: 481 PDETMLKSLASRKLWRQCVKCNNMVELAEGCYHITCRCGYEFCYICGAPWKNKKGTCTCP 540

Query: 541 IWDERNIIRDQRRR 552
           IWDERNIIRDQR++
Sbjct: 541 IWDERNIIRDQRQQ 554

BLAST of Lag0023060 vs. NCBI nr
Match: KAG7019256.1 (dbl4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 949.9 bits (2454), Expect = 9.7e-273
Identity = 464/555 (83.60%), Postives = 502/555 (90.45%), Query Frame = 0

Query: 1   MADTMDDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDD LQD+ITEQRSE+LAAKTL+SDLD A+QLQ+QEAMNASL S  S +   ++N
Sbjct: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63

Query: 61  RASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEELDRRIHDQNFAN
Sbjct: 64  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 123

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSS----SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSS SSS    SSRGLLDS+CFRVYSKGLISE
Sbjct: 124 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISE 183

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGL+IALV
Sbjct: 184 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 243

Query: 241 LGLTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQYI   V PG+SS+ATLVNEV LLQGKFT CNPSLVTRNDIKFA
Sbjct: 244 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 303

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 304 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 363

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VIEI+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 364 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 423

Query: 421 CSALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY++ASY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 424 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 483

Query: 481 RPDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNC 540
           RPDEEMLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC C
Sbjct: 484 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 543

Query: 541 PIWDERNIIRDQRRR 552
           PIWDERNIIR+QR+R
Sbjct: 544 PIWDERNIIRNQRQR 558

BLAST of Lag0023060 vs. NCBI nr
Match: XP_022964983.1 (uncharacterized protein LOC111464930 [Cucurbita moschata] >KAG6583501.1 hypothetical protein SDJN03_19433, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 949.9 bits (2454), Expect = 9.7e-273
Identity = 464/555 (83.60%), Postives = 502/555 (90.45%), Query Frame = 0

Query: 1   MADTMDDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDD LQD+ITEQRSE+LAAKTL+SDLD A+QLQ+QEAMNASL S  S +   ++N
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKGPATVN 60

Query: 61  RASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEELDRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSS----SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSS SSS    SSRGLLDS+CFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISE 180

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGL+IALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 240

Query: 241 LGLTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQYI   V PG+SS+ATLVNEV LLQGKFT CNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VIEI+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY++ASY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 481 RPDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNC 540
           RPDEEMLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC C
Sbjct: 481 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 541 PIWDERNIIRDQRRR 552
           PIWDERNIIR+QR+R
Sbjct: 541 PIWDERNIIRNQRQR 555

BLAST of Lag0023060 vs. NCBI nr
Match: XP_023519052.1 (uncharacterized protein LOC111782521 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 947.6 bits (2448), Expect = 4.8e-272
Identity = 461/555 (83.06%), Postives = 502/555 (90.45%), Query Frame = 0

Query: 1   MADTMDDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDD LQD+ITEQRSE+LAAKTL+SDLD A+QLQ+QEAM+ASL S  S +   S+N
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMDASLGSKSSSKRPASVN 60

Query: 61  RASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEE+DRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEEIDRRIHDQNFAN 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSS----RGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSS SSSSS    RGLLDS+CFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASTRGLLDSECFRVYSKGLISE 180

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGLNIALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLNIALV 240

Query: 241 LGLTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQYI   V PG+SS+AT+VNEV LLQGKFT CNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATVVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFED+  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDICVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VI+I+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIDIISQRIKEASMPVLEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY++ASY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 481 RPDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNC 540
           RPDEEMLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC C
Sbjct: 481 RPDEEMLKSLASKKLWRQCVACNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 541 PIWDERNIIRDQRRR 552
           PIWDERNIIR+QR+R
Sbjct: 541 PIWDERNIIRNQRQR 555

BLAST of Lag0023060 vs. ExPASy Swiss-Prot
Match: P0CE10 (ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01020 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.2e-17
Identity = 56/202 (27.72%), Postives = 90/202 (44.55%), Query Frame = 0

Query: 324  CTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESC 383
            C IC  +V  D  +S++GC H +C +C+ +  E  + N D   + C H  C + I +   
Sbjct: 1561 CPICLSEV--DDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1620

Query: 384  GKFL-ESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQASYIDAERSG 443
               L + K+ E++S  +         K+ +C    C ++        Y  A     E   
Sbjct: 1621 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSI--------YRVAG--PQESGE 1680

Query: 444  ARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKCNNM 503
               C  C+   C  C + +H  +TC  Y+K   NP   +  LK  A  K  ++C  C + 
Sbjct: 1681 PFICGACHSETCTRCHLEYHPLITCERYKKFKENP---DLSLKDWAKGKDVKECPICKST 1740

Query: 504  VELAEGCYHITCRCGYEFCYTC 524
            +E  +GC H+ CRCG   C+TC
Sbjct: 1741 IEKTDGCNHLQCRCGKHICWTC 1747

BLAST of Lag0023060 vs. ExPASy Swiss-Prot
Match: F4KGU4 (ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10370 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 3.7e-17
Identity = 56/202 (27.72%), Postives = 88/202 (43.56%), Query Frame = 0

Query: 324  CTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESC 383
            C IC  +V  D  +S++GC H +C +C+ +  E  + N D   + C H  C + I +   
Sbjct: 1564 CPICLSEV--DDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVLADM 1623

Query: 384  GKFL-ESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQASYIDAERSG 443
               L + K+ E+ S  +         K  +C    C ++        Y  A     E   
Sbjct: 1624 RALLSQEKLDELFSASLSSFVTSSDGKFRFCSTPDCPSV--------YRVAG--PQESGE 1683

Query: 444  ARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKCNNM 503
               C  C+   C  C + +H  +TC  Y+K   NP   +  LK  A  K  ++C  C + 
Sbjct: 1684 PFICGACHSEICTRCHLEYHPLITCERYKKFKENP---DLSLKDWAKGKNVKECPICKST 1743

Query: 504  VELAEGCYHITCRCGYEFCYTC 524
            +E  +GC H+ CRCG   C+TC
Sbjct: 1744 IEKTDGCNHMKCRCGKHICWTC 1750

BLAST of Lag0023060 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 3.7e-17
Identity = 67/240 (27.92%), Postives = 105/240 (43.75%), Query Frame = 0

Query: 323 TCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVN-CPHQGCKSEINIE 382
           +C IC ED    Q F++  C HRYC  C K ++E+++  G   +   CP   CK  ++ +
Sbjct: 138 SCLICLEDYPPTQTFALI-CNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQD 197

Query: 383 SCGKFLESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQASYIDAERS 442
           +  + +  +V E  +  I ++ V    +V +CP   C   +                ER 
Sbjct: 198 AFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGCIYSIRCDR-----------KERK 257

Query: 443 GARKCMKCNLFFCINC---KVPWHYNLTCYDYRK-LNPNPRPDEEMLKSLASRKLWRQCI 502
            A  C KC   +C NC   ++  H    C    K L       E +   LA+ K   +C 
Sbjct: 258 EAVNC-KCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCP 317

Query: 503 KCNNMVELAEGCYHITCR-----CGYEFCYTCGAPWKKKKATC----NCPIWDERNIIRD 548
           +C + +E   GC H+TCR     CG+EFC+ C  PW +  +T     NC  +D+     D
Sbjct: 318 ECRSPIEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKED 361

BLAST of Lag0023060 vs. ExPASy Swiss-Prot
Match: Q9US46 (E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=itt1 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.2e-12
Identity = 56/245 (22.86%), Postives = 95/245 (38.78%), Query Frame = 0

Query: 321 KETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINI 380
           K  C +CF++      F +  C H  C SC++ +  + +  G    + C    C  +  +
Sbjct: 171 KFQCNVCFDEFNGTDCFQLTRCGHVSCQSCLRDYYTMCIQEGMFSQIKCIDLDCGKDAPV 230

Query: 381 ESCGKFLESKVIEIMSQRIKEASVPVLEK-----VYCPYSKCSALMSKSELLKYTQASYI 440
            +  K LES V   ++ R KE       +     ++CP S C                  
Sbjct: 231 LTL-KELESIVGVQLTNRYKELEEKRRYENDSNIIFCPRSFCQG---------------- 290

Query: 441 DAERSGARK---CMKCNLFFCINCKVPWHYNLT--------------CYDYRKLNPNPRP 500
            ++R   +K   C KC+  FC  C+  WH +L+                +Y++  P    
Sbjct: 291 PSKRDPGQKLAICQKCDFAFCSFCQATWHGDLSPCKLEGDSKKLVEMYLNYQENEPEKAL 350

Query: 501 D-------------EEMLKSLASRKLW-----RQCIKCNNMVELAEGCYHITCRCGYEFC 526
           +              E +K+    + W     ++C  C+ +VE  +GC H+ C CG  FC
Sbjct: 351 ELEKRYGKRIIDRLVEQVKNDEEAEKWVLLNGQRCPTCDRVVERIDGCCHMNCLCGTHFC 398

BLAST of Lag0023060 vs. ExPASy Swiss-Prot
Match: Q9LVW9 (Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI4 PE=5 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 8.1e-12
Identity = 47/202 (23.27%), Postives = 84/202 (41.58%), Query Frame = 0

Query: 324 CTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESC 383
           C IC E+  +    +   C HR+C  C K+H  VR+  G+G  + C    C +    +  
Sbjct: 119 CDICMEEDLSKYAMTRMECGHRFCNDCWKEHFTVRINEGEGKRIRCMAYKCNT--ICDEA 178

Query: 384 GKFLESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQASYIDAERSGA 443
            + + +++ E   + + E+ V     V +CP    S     + +        +D      
Sbjct: 179 RQLVSTELAEKFDRFLIESYVEDNNMVKWCP----STPHCGNAIRNIKDDGDVDEVE--- 238

Query: 444 RKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKCNNMV 503
                C L FC +C    H   +C  ++        + E +  +      + C KC+  +
Sbjct: 239 ---CSCGLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNT--KLCPKCSKPI 298

Query: 504 ELAEGCYHITCRCGYEFCYTCG 525
           +  +GC H+TC+CG  FC+ CG
Sbjct: 299 QKRDGCNHMTCKCGQHFCWLCG 306

BLAST of Lag0023060 vs. ExPASy TrEMBL
Match: A0A6J1DZJ9 (uncharacterized protein LOC111024540 OS=Momordica charantia OX=3673 GN=LOC111024540 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.4e-277
Identity = 474/554 (85.56%), Postives = 505/554 (91.16%), Query Frame = 0

Query: 1   MADTM-DDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADTM DDD LQ++++EQRSELLAAKTL SDLD AFQLQLQEAMNASL S PS +PSPS 
Sbjct: 1   MADTMDDDDYLQEVVSEQRSELLAAKTLFSDLDLAFQLQLQEAMNASLDSKPSTQPSPSR 60

Query: 61  NRASPVLESEE-TGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNF 120
           NR  P LESE+ +  LDLATTLMLEDIARFAMEFKDRE+C TEMRKMKEELDRRIHDQNF
Sbjct: 61  NRVVPKLESEDSSSALDLATTLMLEDIARFAMEFKDREQCHTEMRKMKEELDRRIHDQNF 120

Query: 121 ANYILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEER 180
           A YI NVPEDEWR+YGDNYEKPY  SS SSSSS SSS +R  LDS+ FRVYSKGLISEER
Sbjct: 121 ATYIRNVPEDEWRRYGDNYEKPYSGSSGSSSSSSSSSPARVCLDSEIFRVYSKGLISEER 180

Query: 181 IRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLG 240
           IRDM+VRVA IGVA+CDPKDNLL EKM PLE VVDGKEI+SECAELEALVEGLN+AL+LG
Sbjct: 181 IRDMKVRVAAIGVAVCDPKDNLLFEKMMPLESVVDGKEINSECAELEALVEGLNVALILG 240

Query: 241 LTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFR 300
           L NVTFFCADYMLYQY+TGRVPPGASSLATLVNEV+LLQ +FT CNPSLVTRND+KFAF+
Sbjct: 241 LKNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVILLQRRFTYCNPSLVTRNDMKFAFK 300

Query: 301 LAREAIVSQITWPAEADNGKCL-KETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEV 360
           LAREAIVSQITWP EA NGKCL KETC ICFEDVR DQMFS+DGCLHRYCFSCMKQHVEV
Sbjct: 301 LAREAIVSQITWPTEAGNGKCLKKETCIICFEDVRVDQMFSVDGCLHRYCFSCMKQHVEV 360

Query: 361 RLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKC 420
           RLLNG+GM VNCPHQGC S INIESCGKFLE KVIEIMSQRIKEASVP+LEKVYCPYS+C
Sbjct: 361 RLLNGNGMKVNCPHQGCTSGINIESCGKFLEPKVIEIMSQRIKEASVPILEKVYCPYSRC 420

Query: 421 SALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPR 480
           SALMSKSELLKYT+AS+I AE+SGARKCMKCN FFCINCKVPWHYNLTCYDYRK NPNPR
Sbjct: 421 SALMSKSELLKYTEASFIGAEQSGARKCMKCNQFFCINCKVPWHYNLTCYDYRKSNPNPR 480

Query: 481 PDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCP 540
           PDE MLKSLASRKLWRQC+KCNNMVELAEGCYHITCRCGYEFCY CGAPWK KK TC CP
Sbjct: 481 PDETMLKSLASRKLWRQCVKCNNMVELAEGCYHITCRCGYEFCYICGAPWKNKKGTCTCP 540

Query: 541 IWDERNIIRDQRRR 552
           IWDERNIIRDQR++
Sbjct: 541 IWDERNIIRDQRQQ 554

BLAST of Lag0023060 vs. ExPASy TrEMBL
Match: A0A6J1HMI1 (uncharacterized protein LOC111464930 OS=Cucurbita moschata OX=3662 GN=LOC111464930 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.7e-273
Identity = 464/555 (83.60%), Postives = 502/555 (90.45%), Query Frame = 0

Query: 1   MADTMDDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDD LQD+ITEQRSE+LAAKTL+SDLD A+QLQ+QEAMNASL S  S +   ++N
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKGPATVN 60

Query: 61  RASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEELDRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSS----SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSS SSS    SSRGLLDS+CFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISE 180

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGL+IALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 240

Query: 241 LGLTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQYI   V PG+SS+ATLVNEV LLQGKFT CNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VIEI+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY++ASY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 481 RPDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNC 540
           RPDEEMLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC C
Sbjct: 481 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 541 PIWDERNIIRDQRRR 552
           PIWDERNIIR+QR+R
Sbjct: 541 PIWDERNIIRNQRQR 555

BLAST of Lag0023060 vs. ExPASy TrEMBL
Match: A0A5A7U9A4 (Putative E3 ubiquitin-protein ligase rbrA OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold769G00460 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 455/546 (83.33%), Postives = 496/546 (90.84%), Query Frame = 0

Query: 1   MADTM-DDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADT+ DDDC+Q++IT+QRSELL+AKTL SDLD+AFQLQLQEAM+ASL S+PSIE S SL
Sbjct: 1   MADTLNDDDCVQELITQQRSELLSAKTLFSDLDYAFQLQLQEAMDASLTSIPSIESSSSL 60

Query: 61  NRASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFA 120
           N A PVL+SEET  LDLATTLMLEDIARFAMEFKDRE CQTEMRKMKEELDRRIHDQ FA
Sbjct: 61  NTADPVLDSEETSGLDLATTLMLEDIARFAMEFKDREHCQTEMRKMKEELDRRIHDQKFA 120

Query: 121 NYILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEERI 180
            YI +VPE+EWR+YGDNYEKPYGESSSSSSSS S +     +DS+CFRVYSKGLISEERI
Sbjct: 121 EYIRSVPENEWREYGDNYEKPYGESSSSSSSSSSWN-----VDSECFRVYSKGLISEERI 180

Query: 181 RDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGL 240
           RD  VRVAGIG+A+CDPKDNLL EKM P+E +V+GKE S+ECAELEALVEGLN+AL+LGL
Sbjct: 181 RDTVVRVAGIGIAVCDPKDNLLFEKMSPIESMVEGKETSNECAELEALVEGLNVALILGL 240

Query: 241 TNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRL 300
            ++TFFCADYMLYQY+TGRVPP  SS A LVNEVV+LQGKFT CNPSLVTRNDIKFAF+L
Sbjct: 241 KSITFFCADYMLYQYLTGRVPPATSSTAKLVNEVVVLQGKFTYCNPSLVTRNDIKFAFKL 300

Query: 301 AREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRL 360
           AREAIVSQITWPAEADNG CLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQHVEV+L
Sbjct: 301 AREAIVSQITWPAEADNGNCLKETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHVEVKL 360

Query: 361 LNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSA 420
           LNG+GM  NCPHQGC SE+NIESCGKFLE KV EIMSQRIKEASVP+ EKVYCPYS+CSA
Sbjct: 361 LNGNGMQANCPHQGCTSEVNIESCGKFLEPKVFEIMSQRIKEASVPIQEKVYCPYSRCSA 420

Query: 421 LMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPD 480
           LMSK+ELL+YT+ASYIDAER+GARKCMKCN FFCINCKV WHYNLTCYDYR LNPN  PD
Sbjct: 421 LMSKTELLRYTEASYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDYRNLNPNLHPD 480

Query: 481 EEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCPIW 540
           E+MLKSLA+RKLWRQCI CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC CPIW
Sbjct: 481 EQMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCKCPIW 540

Query: 541 DERNII 546
           DERNII
Sbjct: 541 DERNII 541

BLAST of Lag0023060 vs. ExPASy TrEMBL
Match: A0A1S3CJH8 (uncharacterized protein LOC103501665 OS=Cucumis melo OX=3656 GN=LOC103501665 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 455/546 (83.33%), Postives = 496/546 (90.84%), Query Frame = 0

Query: 1   MADTM-DDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADT+ DDDC+Q++IT+QRSELL+AKTL SDLD+AFQLQLQEAM+ASL S+PSIE S SL
Sbjct: 1   MADTLNDDDCVQELITQQRSELLSAKTLFSDLDYAFQLQLQEAMDASLTSIPSIESSSSL 60

Query: 61  NRASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFA 120
           N A PVL+SEET  LDLATTLMLEDIARFAMEFKDRE CQTEMRKMKEELDRRIHDQ FA
Sbjct: 61  NTADPVLDSEETSGLDLATTLMLEDIARFAMEFKDREHCQTEMRKMKEELDRRIHDQKFA 120

Query: 121 NYILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEERI 180
            YI +VPE+EWR+YGDNYEKPYGESSSSSSSS S +     +DS+CFRVYSKGLISEERI
Sbjct: 121 EYIRSVPENEWREYGDNYEKPYGESSSSSSSSSSWN-----VDSECFRVYSKGLISEERI 180

Query: 181 RDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGL 240
           RD  VRVAGIG+A+CDPKDNLL EKM P+E +V+GKE S+ECAELEALVEGLN+AL+LGL
Sbjct: 181 RDTVVRVAGIGIAVCDPKDNLLFEKMSPIESMVEGKETSNECAELEALVEGLNVALILGL 240

Query: 241 TNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRL 300
            ++TFFCADYMLYQY+TGRVPP  SS A LVNEVV+LQGKFT CNPSLVTRNDIKFAF+L
Sbjct: 241 KSITFFCADYMLYQYLTGRVPPATSSTAKLVNEVVVLQGKFTYCNPSLVTRNDIKFAFKL 300

Query: 301 AREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRL 360
           AREAIVSQITWPAEADNG CLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQHVEV+L
Sbjct: 301 AREAIVSQITWPAEADNGNCLKETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHVEVKL 360

Query: 361 LNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSA 420
           LNG+GM  NCPHQGC SE+NIESCGKFLE KV EIMSQRIKEASVP+ EKVYCPYS+CSA
Sbjct: 361 LNGNGMQANCPHQGCTSEVNIESCGKFLEPKVFEIMSQRIKEASVPIQEKVYCPYSRCSA 420

Query: 421 LMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPD 480
           LMSK+ELL+YT+ASYIDAER+GARKCMKCN FFCINCKV WHYNLTCYDYR LNPN  PD
Sbjct: 421 LMSKTELLRYTEASYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDYRNLNPNLHPD 480

Query: 481 EEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCPIW 540
           E+MLKSLA+RKLWRQCI CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC CPIW
Sbjct: 481 EQMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCKCPIW 540

Query: 541 DERNII 546
           DERNII
Sbjct: 541 DERNII 541

BLAST of Lag0023060 vs. ExPASy TrEMBL
Match: A0A0A0LXM8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G088460 PE=3 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.7e-268
Identity = 456/547 (83.36%), Postives = 495/547 (90.49%), Query Frame = 0

Query: 1   MADTM-DDDCLQDIITEQRSELLAAKTLVSDLDFAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADTM DDDC+QD+IT+QRSE+L+AKTL SDLDFAFQLQLQEAM+ASL S PS + S SL
Sbjct: 1   MADTMNDDDCVQDLITQQRSEILSAKTLFSDLDFAFQLQLQEAMDASLASKPSTDSSSSL 60

Query: 61  NRASPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFA 120
           N   PVL+SE+T  LDLATTLMLEDIARFAMEFKDRE+CQTEMRKMKEELDRRIHDQ FA
Sbjct: 61  NTPDPVLDSEDTSGLDLATTLMLEDIARFAMEFKDREQCQTEMRKMKEELDRRIHDQKFA 120

Query: 121 NYILNVPEDEWRKYGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEERI 180
            +I +VPE+EWR+YGDNYEKPYGESSSSSSSS SSS S   +DS+C RVYSKGLISEERI
Sbjct: 121 EFIRSVPENEWREYGDNYEKPYGESSSSSSSSSSSSWS---VDSECCRVYSKGLISEERI 180

Query: 181 RDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGL 240
           RDM VRVAGIGVA+CDPKDNLL EKM P+E +V+GKE SSECAELEALVEGLN+AL+LGL
Sbjct: 181 RDMVVRVAGIGVAVCDPKDNLLFEKMSPIESMVEGKETSSECAELEALVEGLNVALILGL 240

Query: 241 TNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRL 300
             VTFFCADYMLYQY+TGRVPP  SS   LVNEVV+LQGKFT CNPSLVTRNDIKFAF+L
Sbjct: 241 KTVTFFCADYMLYQYLTGRVPPATSSTEKLVNEVVVLQGKFTYCNPSLVTRNDIKFAFKL 300

Query: 301 AREAIVSQITWPAEADNGKCLK-ETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVR 360
           AREAIVSQITWPA+A+NGKCLK ETCTICFEDV  DQMFS+DGCLHRYCFSCMKQHVEVR
Sbjct: 301 AREAIVSQITWPAQAENGKCLKEETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHVEVR 360

Query: 361 LLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCS 420
           LLNG+GM  NCPHQGC SE+NIESCGKFLE KV EIMSQRIKEASVPV EKVYCPYS+CS
Sbjct: 361 LLNGNGMQANCPHQGCTSELNIESCGKFLEPKVFEIMSQRIKEASVPVQEKVYCPYSRCS 420

Query: 421 ALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRP 480
           ALMSK++LL+YT+A YIDAER+GARKCMKCN FFCINCKV WHYNLTCYD+RKLNPN  P
Sbjct: 421 ALMSKTDLLRYTEAFYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDHRKLNPNLHP 480

Query: 481 DEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCPI 540
           DE+MLKSLA+RKLWRQCI CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC CPI
Sbjct: 481 DEKMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCKCPI 540

Query: 541 WDERNII 546
           WDERNII
Sbjct: 541 WDERNII 544

BLAST of Lag0023060 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 391.7 bits (1005), Expect = 9.5e-109
Identity = 219/535 (40.93%), Postives = 317/535 (59.25%), Query Frame = 0

Query: 30  DLDFAFQLQLQEAMNASLKSM---PSIEPSPSLNRASPVLESEETGPLDLATTLMLEDIA 89
           D ++AF+LQ++EA+ ASL S    P   PSP +     VL   E  P D  T    +  +
Sbjct: 4   DSEYAFRLQMEEALAASLSSQSRTPQRPPSPPIVATCGVL-IVENNPNDSKTA---KTSS 63

Query: 90  RFAMEFKDRERCQTEMRKMKEELDRRIHDQNFANYILNVPEDEWRKYGDNYEKPYGESSS 149
            +  +FK          K K          +  N  + +        G    KP  + + 
Sbjct: 64  GYGFDFKRAVGGGNSKAKGKTHESVTGVRTDERNPNIGLGNSRSTSTGHGNTKPLPQETV 123

Query: 150 SSSSSLSSSSSR-----GLLDSQ-CFRVYSKGLISEERIR-DMQVRVAGIGVAICDPKDN 209
             +  +   SSR     G ++    +R+Y KGL+S+E  +  M   V+G GVAICD +DN
Sbjct: 124 RPAQLVGEGSSRVNTAAGKINGNLLYRLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDN 183

Query: 210 LLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYITGRV 269
           LL E   PL   +D   +S + AEL+AL+ GL  AL LG+ ++ FFC  Y ++QY+TG+ 
Sbjct: 184 LLFEMKGPL---ID-NGMSRQGAELKALIRGLTEALKLGIKHIVFFCDSYPIFQYVTGKW 243

Query: 270 PPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKC 329
                 ++ L++++  +   F+     LV RND+KFA++LARE+I+S +T   +    K 
Sbjct: 244 MAKQKKISLLLDDLQSIMQHFSSYQHVLVARNDVKFAYKLARESILSMVTPHEDPRQAKA 303

Query: 330 -LKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEI 389
            LKE C ICF D+ A+ MFS+D C HR+CF C+KQHVEV+LL+  GM   CPH GCKSE+
Sbjct: 304 VLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLH--GMAPKCPHDGCKSEL 363

Query: 390 NIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQASYIDAE 449
            I++CGK L  K+ ++  QR++E ++PV E+VYCPY +CSALMSK+++ +  ++      
Sbjct: 364 VIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYP 423

Query: 450 RSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKC 509
           +SG R+C++C   FC++CKVPWH NL+C +Y+KL+P P  D+  LKSLA+ K+WRQC KC
Sbjct: 424 KSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPADDVKLKSLANNKMWRQCGKC 483

Query: 510 NNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATC--NCPIWDERNIIRDQRRR 552
            +M+EL++GC HITCRCG+EFCY CG  W K   TC   CP W+E  I R    R
Sbjct: 484 QHMIELSQGCNHITCRCGHEFCYNCGGGWNKIMGTCLNRCPTWNEEYITRQDPAR 528

BLAST of Lag0023060 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 7.8e-87
Identity = 173/385 (44.94%), Postives = 236/385 (61.30%), Query Frame = 0

Query: 148 SSSSLSSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRV--AGIGVAICDPKDNLLLEKM 207
           +S    SS +   +D+  +R+  KGL++ E + D   +   AG GVAICD KDNLL E  
Sbjct: 136 NSKGEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK 195

Query: 208 KPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYITGRVPPGASS 267
           + L  +    EIS    E+ ALV GL+ +  LG+ NV  +C D  +YQ I GR       
Sbjct: 196 ESLRDI----EISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGR-GKSKKK 255

Query: 268 LATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCT 327
           +  LV EV  +  K    +  LV RND+KFAFRLAREAI  + +    A+ G    ETC 
Sbjct: 256 IDHLVEEVQGILEKMACIDAVLVARNDVKFAFRLAREAI-GRNSVDVNAEQG----ETCG 315

Query: 328 ICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGK 387
           I FE+   + MF  + CLHR+CF C+KQHV+V+L +  G    C   GCK ++ +E C K
Sbjct: 316 IFFEETDVEHMFVTEKCLHRHCFPCVKQHVKVKLRS--GTEPTCLEYGCKFKLTLERCSK 375

Query: 388 FLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQASYIDAERSGARKC 447
            L  K+IE+  Q++KE S+P  E++YCPY  CS LMSK+EL         +A+ S  R C
Sbjct: 376 VLTLKLIEMWKQKMKEDSIPAAERIYCPYPNCSMLMSKTELSS-------EADLSNVRTC 435

Query: 448 MKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKCNNMVELA 507
           +KC   FCI+CKVP H +L+  DY+KL+P+P  D+  LKSLA+ K+WRQC+KC +M+EL+
Sbjct: 436 VKCCGLFCIDCKVPSHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELS 495

Query: 508 EGCYHITCRCGYEFCYTCGAPWKKK 531
            GC H+TCRCGYEFCY CG  W+K+
Sbjct: 496 HGCNHMTCRCGYEFCYECGIEWQKR 501

BLAST of Lag0023060 vs. TAIR 10
Match: AT3G45580.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 300.4 bits (768), Expect = 2.9e-81
Identity = 160/370 (43.24%), Postives = 226/370 (61.08%), Query Frame = 0

Query: 166 FRVYSKGLISEERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELE 225
           +R+Y KGL+SEE +      +AG GVAICD  DNLL + MK  E V D +    E  E+ 
Sbjct: 28  YRLYFKGLVSEETVE----LLAGFGVAICDKDDNLLFQ-MK--EQVHDSRVTVLE-VEIM 87

Query: 226 ALVEGLNIALVLGLTNVTFFCADYMLYQYITGRVPPGASSLATLVNEVVLLQGKFTVCNP 285
           AL  GL  A+ LG+ N++ +   Y +++ +  +      + A L++ V  ++ + T   P
Sbjct: 88  ALKRGLTEAVGLGIDNISIYSDHYRIFELVMEKSASAEENFALLMDNVQHIRQRLTSSFP 147

Query: 286 SLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCTICFED-VRADQMFSIDGCLH 345
            LVTRN IKF + LA E IVS+I+     D+ K    TC+IC +D    + MFS+  C H
Sbjct: 148 VLVTRNQIKFVYELAMETIVSEISIHI-PDHDK----TCSICSDDNFEPELMFSVALCGH 207

Query: 346 RYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASV 405
            +C  C+K+H+EVRLL G   V  C H  C+S++ + +C   L SK+  +   RI+E S+
Sbjct: 208 EFCVECVKRHIEVRLLAGG--VPRCLHYQCESKLTLANCANLLTSKLKAMWELRIEEESI 267

Query: 406 PVLEKVYCPYSKCSALMSKSELLKYTQASYIDAERSGARKCMKCNLFFCINCKVPWHYNL 465
           PV E+VYCP  +CS+LMS ++L   T+      E    R C+KC   FCINCK+PWH NL
Sbjct: 268 PVEERVYCPNPRCSSLMSVTKLSNSTR------EDVTMRSCVKCGEPFCINCKLPWHSNL 327

Query: 466 TCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCG 525
           +C DY+ L PNP  D+  LK+LA++K+WRQC  C N++EL+EGC HITCRCG++FCY CG
Sbjct: 328 SCNDYKSLGPNPTADDIKLKALANQKMWRQCENCKNVIELSEGCMHITCRCGHQFCYKCG 376

Query: 526 APWKKKKATC 535
           A W   +  C
Sbjct: 388 AKWITGRVFC 376

BLAST of Lag0023060 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 6.4e-81
Identity = 169/411 (41.12%), Postives = 239/411 (58.15%), Query Frame = 0

Query: 133 YGDNYEKPYGESSSSSSSSLSSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRV-AGIGV 192
           Y    + P  +   SS    S+  ++  +D+  +++Y KGL+S E   D +  V  G GV
Sbjct: 3   YAVTKDIPNRKGEGSSKVGQSTFETQSCVDNVVYKLYFKGLVSGETATDKKAIVKVGFGV 62

Query: 193 AICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYML 252
           AICD  DNL     K L   V    I+ E A++ AL+ GL  ++  G+ NV   C D  +
Sbjct: 63  AICDEADNLFYGMNKSLYEAV----INREEADILALITGLYESIHRGVKNVVICCDDRQI 122

Query: 253 YQYITGRVPPGASSLATLVNEVVLLQGKFTVCNPSLVTRNDIKFAFRLAREAIVSQITWP 312
           YQ I GR  P   ++  L+ EV  L+G+       LV   D  FA RLA +A+V      
Sbjct: 123 YQTIIGREKPQQKNV-HLLEEVQRLRGRLASTGTVLVATRDDNFALRLAIDALV------ 182

Query: 313 AEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPH 372
            +A   K L  TC+IC +   A+ M   D CLHR+CFSC+KQ V+V+L +  G+V  C  
Sbjct: 183 -KATQEKPL--TCSICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRS--GIVPPCLE 242

Query: 373 QGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQ 432
            GCKSE+ +ESC   L  K+IE+  ++++E  +P  EK+YCPY  CS LMSK+EL +   
Sbjct: 243 DGCKSELTLESCSMVLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTELSR--- 302

Query: 433 ASYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKL 492
               +AE+S  R C+KC+  FCI+CKVPWH +L+C DY++++     ++ MLK LA+ ++
Sbjct: 303 ----EAEQSNVRACIKCSELFCIDCKVPWHSDLSCADYKRIHSERLVNDMMLKVLANDQM 362

Query: 493 WRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCNCPIWDER 543
           WRQC +C +M+EL EGC HITCRCGYEFCY CG  W K     +C + D R
Sbjct: 363 WRQCSECKHMIELTEGCNHITCRCGYEFCYRCGHKWTKYH-HYSCQLMDNR 389

BLAST of Lag0023060 vs. TAIR 10
Match: AT2G21420.1 (IBR domain containing protein )

HSP 1 Score: 296.6 bits (758), Expect = 4.2e-80
Identity = 164/379 (43.27%), Postives = 225/379 (59.37%), Query Frame = 0

Query: 153 SSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRV-AGIGVAICDPKDNLLLEKMKPLEYV 212
           S+S+S    DS  +R++ KGL+S+E   DM+  V AG G+AICD  + LL    K L   
Sbjct: 37  SASASNIFADSLVYRLFFKGLVSDETTTDMEEIVKAGFGIAICDEANTLLYNMKKSLN-- 96

Query: 213 VDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYITGRVPPGASSLATLVN 272
                I+ E  E++AL+  LN+++ + L NV   C DY ++Q +TGR  P   ++  LV 
Sbjct: 97  -GDDVINPEEVEIKALICVLNVSIQMELRNVMICCGDYQIFQILTGRGKP-QQNIVHLVE 156

Query: 273 EVVLLQGKFTVCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCTICFEDV 332
           +V  L+GK +     LV R D+     LA EAI                 ETC IC E+ 
Sbjct: 157 QVQHLRGKLSSTEVVLVPRADV---IILAIEAIGG---------------ETCCICRENT 216

Query: 333 RADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKV 392
            AD+MF  + C HR CFSC+ +HV+  LL   G+   C H  C SE+  ESC K L   +
Sbjct: 217 DADRMFFTENCFHRQCFSCVNRHVQRMLL--CGISPTCLHFPCNSELTFESCSKVLTPNL 276

Query: 393 IEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQASYIDAERSGARKCMKCNLF 452
           IE   ++I+E  VP  +K+YCPY +CS LMSK+ L + T       ++S  R C+KC   
Sbjct: 277 IEFWKRKIEEDLVPAADKIYCPYRRCSMLMSKTALSRET-------DQSNVRACIKCCRL 336

Query: 453 FCINCKVPWHYNLTCYDYRKLNPNPRPDEEMLKSLASRKLWRQCIKCNNMVELAEGCYHI 512
           FCI+CKVP H  L+C DY+KLNP+P  D + LKSLA++K WRQC++C+N+VEL EGC HI
Sbjct: 337 FCIDCKVPSHAGLSCVDYKKLNPDPLYDVK-LKSLANKKKWRQCVQCSNLVELFEGCNHI 383

Query: 513 TCRCGYEFCYTCGAPWKKK 531
           TCRCG+EFCY CG  W ++
Sbjct: 397 TCRCGFEFCYVCGKEWNQR 383