Lag0023023 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0023023
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr7: 42996874 .. 42997596 (-)
RNA-Seq ExpressionLag0023023
SyntenyLag0023023
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCGTGAATTGTATGACAGTTGAGATGGCAAAAGTGTTGGGAGCGGTGGTGGGGGTGGTTGAGAAGTTGAGGGTGAGGGGAAGAATGGGAGGACTGGCCTATAATGAGGGTGAGGGTAGTAGTGAATATGAAGAACCCATGCTGAGAGGGGTGAAGATAAAAACAAAAGAGGGAAAAGAGGTTTGGTGCCCAATTCAGTATGAAAAATTGCCGGATATGTGCTTCAAATGTGGGATCATTGGGCACTCTTATCGTGAGTGTCAAGCCACGCGGGGGGGGGGAGAGGGGGAGGATCGACCTTATGGGGAGTGGATGAGGGCAATCCCGAGTAGGGGGAGTGGGAGGAAGGGCAGAGGAAGGAAGAGGGAAGAGTGTCCAATGGGGTTCGGTGGGCAGGGTGGTGGGAAAAGTGGGGGGACGGAGGGAGGACTGGGTGAGGGGAGTGGTCTGGTAAAGCATGCTGGGGAAAAGGGCTAGTTTGGGGAATGAGGGGCAAAGGGGCGGTGGGAAGGTGGTGGAGTTAGTCAGGGGGTAAGGAAAGGGGTCAAGGTGGGAGAGGGCAAAAGGGAAGGAGGTGATGGGGGATCAAGGTGTGGAATTGTCGGAGAGGGGAGGGGGTACCTTTGGGAAGGAGGAGTGCATCTTACCCATCTGTCTGTTGGGGATGGGGTGAGGAAGTGGAAAAGAGGGAGAGGGAGGTGGTGGATGAAATGGGGCTGA

mRNA sequence

ATGCCCGTGAATTGTATGACAGTTGAGATGGCAAAAGTGTTGGGAGCGGTGGTGGGGGTGGTTGAGAAGTTGAGGGTGAGGGGAAGAATGGGAGGACTGGCCTATAATGAGGGTGAGGGTAGTAGTGAATATGAAGAACCCATGCTGAGAGGGGTGAAGATAAAAACAAAAGAGGGAAAAGAGGTTTGGTGCCCAATTCAGTATGAAAAATTGCCGGATATGTGCTTCAAATGTGGGATCATTGGGCACTCTTATCGTGAGTGTCAAGCCACGCGGGGGGGGGGAGAGGGGGAGGATCGACCTTATGGGGAGTGGATGAGGGCAATCCCGAGTAGGGGGAGTGGGAGGAAGGGCAGAGGAAGGAAGAGGGAAGAGTGTCCAATGGGGTTCGGTGGGCAGGGTGGTGGGAAAAGTGGGGGGACGGAGGGAGGACTGGGTGAGGGGAGTGGTCTGGGGGTAAGGAAAGGGGTCAAGGTGGGAGAGGGCAAAAGGGAAGGAGGTGATGGGGGATCAAGGTGTGGAATTGTCGGAGAGGGGAGGGGGTACCTTTGGGAAGGAGGAGTGCATCTTACCCATCTGTCTGTTGGGGATGGGGTGAGGAAGTGGAAAAGAGGGAGAGGGAGGTGGTGGATGAAATGGGGCTGA

Coding sequence (CDS)

ATGCCCGTGAATTGTATGACAGTTGAGATGGCAAAAGTGTTGGGAGCGGTGGTGGGGGTGGTTGAGAAGTTGAGGGTGAGGGGAAGAATGGGAGGACTGGCCTATAATGAGGGTGAGGGTAGTAGTGAATATGAAGAACCCATGCTGAGAGGGGTGAAGATAAAAACAAAAGAGGGAAAAGAGGTTTGGTGCCCAATTCAGTATGAAAAATTGCCGGATATGTGCTTCAAATGTGGGATCATTGGGCACTCTTATCGTGAGTGTCAAGCCACGCGGGGGGGGGGAGAGGGGGAGGATCGACCTTATGGGGAGTGGATGAGGGCAATCCCGAGTAGGGGGAGTGGGAGGAAGGGCAGAGGAAGGAAGAGGGAAGAGTGTCCAATGGGGTTCGGTGGGCAGGGTGGTGGGAAAAGTGGGGGGACGGAGGGAGGACTGGGTGAGGGGAGTGGTCTGGGGGTAAGGAAAGGGGTCAAGGTGGGAGAGGGCAAAAGGGAAGGAGGTGATGGGGGATCAAGGTGTGGAATTGTCGGAGAGGGGAGGGGGTACCTTTGGGAAGGAGGAGTGCATCTTACCCATCTGTCTGTTGGGGATGGGGTGAGGAAGTGGAAAAGAGGGAGAGGGAGGTGGTGGATGAAATGGGGCTGA

Protein sequence

MPVNCMTVEMAKVLGAVVGVVEKLRVRGRMGGLAYNEGEGSSEYEEPMLRGVKIKTKEGKEVWCPIQYEKLPDMCFKCGIIGHSYRECQATRGGGEGEDRPYGEWMRAIPSRGSGRKGRGRKREECPMGFGGQGGGKSGGTEGGLGEGSGLGVRKGVKVGEGKREGGDGGSRCGIVGEGRGYLWEGGVHLTHLSVGDGVRKWKRGRGRWWMKWG
Homology
BLAST of Lag0023023 vs. NCBI nr
Match: XP_022149484.1 (uncharacterized protein LOC111017902 [Momordica charantia])

HSP 1 Score: 91.7 bits (226), Expect = 8.5e-15
Identity = 55/149 (36.91%), Postives = 82/149 (55.03%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKLRVRGRMGGLA-YNEGEGSSEYEEPMLRGVKIKTKEG 60
           +P  C++ EMA +LGA +G VE++   G  G    +       +  +P+ RG+K+K  +G
Sbjct: 132 IPFECISTEMANILGAKLGDVEEIEGDGADGWAGPFIRVRVKIDVSKPLRRGIKLKNSDG 191

Query: 61  KEVWCPIQYEKLPDMCFKCGIIGHSYREC-QATRGGGEGEDRPYGEWMRAIPSRGS---- 120
           K++WCP++YEKLPD C++CG IGHS REC Q ++         YG+W+RA   + S    
Sbjct: 192 KDIWCPLRYEKLPDFCYECGKIGHSGRECEQRSKVVTTNSPEQYGDWLRATLLKKSVSHP 251

Query: 121 --------GRKGRGRKREECPMGFGGQGG 136
                   GR GRG +        GG+GG
Sbjct: 252 EEEVFWRGGRFGRGHQVN------GGRGG 274

BLAST of Lag0023023 vs. NCBI nr
Match: XP_022156711.1 (uncharacterized protein LOC111023555 [Momordica charantia])

HSP 1 Score: 80.9 bits (198), Expect = 1.5e-11
Identity = 40/98 (40.82%), Postives = 56/98 (57.14%), Query Frame = 0

Query: 5   CMTVEMAKVLGAVVGVVEKLRVRGRMGGLAYNEGEGSSEYE---------------EPML 64
           CMT +MAK LGA +G VE              E +G S Y+               +P+ 
Sbjct: 136 CMTKDMAKFLGARLGEVE--------------EVDGMSSYDWVRPFLWVRVKINVLKPLR 195

Query: 65  RGVKIKTKEGKEVWCPIQYEKLPDMCFKCGIIGHSYRE 88
           RG+K+KT +GK++WCP++YE+LPD C+ CG +GHS RE
Sbjct: 196 RGLKVKTSDGKDIWCPLRYERLPDFCYGCGCVGHSIRE 219

BLAST of Lag0023023 vs. NCBI nr
Match: XP_042952130.1 (uncharacterized protein At4g02000-like [Carya illinoinensis])

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-10
Identity = 42/112 (37.50%), Postives = 65/112 (58.04%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKLRVRGRMGGL-AYNEGEGSSEYEEPMLRGVKIKTKEG 60
           MP++CMT +  + +G+ VG VEK+ V+    G   Y   +   +  +PM RG  +  K G
Sbjct: 132 MPLSCMTEKRGEQIGSSVGKVEKVDVQEDGSGWGCYLRVQIHMDITQPMARGRTLIVK-G 191

Query: 61  KEVWCPIQYEKLPDMCFKCGIIGHSYRECQATRGGGE--GEDRPYGEWMRAI 110
           +++W P  YEK+P +CF CG I H    C+    GGE  G++  YG+W+RA+
Sbjct: 192 QKIWIPFSYEKMPRICFTCGCIEHGKEGCKV---GGEAGGQENQYGQWLRAV 239

BLAST of Lag0023023 vs. NCBI nr
Match: XP_015380691.1 (uncharacterized protein LOC107174364 [Citrus sinensis])

HSP 1 Score: 75.9 bits (185), Expect = 4.8e-10
Identity = 44/130 (33.85%), Postives = 67/130 (51.54%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKLRVRGRMGGLAYNEGEGSSEYEE---------PMLRG 60
           +P+ CM  E+ + LG ++G VE++             GE   E+           P+ + 
Sbjct: 112 VPIACMEKELIQELGGMIGAVEEIETD--------ENGECLGEFARIRVLINITLPLKKI 171

Query: 61  VKIKTKEGKEVWCPIQYEKLPDMCFKCGIIGHSYRECQATRGGGEGEDRPYGEWMRAIPS 120
           + +K +   ++  P+ YE+LPD C+ CGIIGH Y+EC A   G + E  PYG WM+AI  
Sbjct: 172 LFLKQEGESDIQMPVVYERLPDFCYCCGIIGHQYKEC-AKFQGQQKEQLPYGGWMKAIII 231

Query: 121 RGSGRKGRGR 122
            G  RK R +
Sbjct: 232 GGRARKNRNK 232

BLAST of Lag0023023 vs. NCBI nr
Match: XP_023913699.1 (uncharacterized protein LOC112025279 [Quercus suber])

HSP 1 Score: 75.5 bits (184), Expect = 6.3e-10
Identity = 41/113 (36.28%), Postives = 64/113 (56.64%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKLRVRGRMGGLAYNEGEGSSEYEEPMLRGVKIKTKEGK 60
           +P N MT E+A+ L   VG+V++      M G ++   +   +   P+ RG +I   EG+
Sbjct: 127 IPANFMTREVAEKLCENVGMVDRTTDFSEMVGGSFMRVKVVIDVALPLCRGRRITLNEGE 186

Query: 61  EVWCPIQYEKLPDMCFKCGIIGHSYRECQ---ATRGGGEGEDRPYGEWMRAIP 111
           E W   +YE+LP++C  CG + HS R+C     + G  + EDR YG W+RA+P
Sbjct: 187 EGWVSFKYERLPNICCWCGCLNHSDRDCDRWIESDGSLKVEDREYGPWIRAVP 239

BLAST of Lag0023023 vs. ExPASy TrEMBL
Match: A0A6J1D765 (uncharacterized protein LOC111017902 OS=Momordica charantia OX=3673 GN=LOC111017902 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.1e-15
Identity = 55/149 (36.91%), Postives = 82/149 (55.03%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKLRVRGRMGGLA-YNEGEGSSEYEEPMLRGVKIKTKEG 60
           +P  C++ EMA +LGA +G VE++   G  G    +       +  +P+ RG+K+K  +G
Sbjct: 132 IPFECISTEMANILGAKLGDVEEIEGDGADGWAGPFIRVRVKIDVSKPLRRGIKLKNSDG 191

Query: 61  KEVWCPIQYEKLPDMCFKCGIIGHSYREC-QATRGGGEGEDRPYGEWMRAIPSRGS---- 120
           K++WCP++YEKLPD C++CG IGHS REC Q ++         YG+W+RA   + S    
Sbjct: 192 KDIWCPLRYEKLPDFCYECGKIGHSGRECEQRSKVVTTNSPEQYGDWLRATLLKKSVSHP 251

Query: 121 --------GRKGRGRKREECPMGFGGQGG 136
                   GR GRG +        GG+GG
Sbjct: 252 EEEVFWRGGRFGRGHQVN------GGRGG 274

BLAST of Lag0023023 vs. ExPASy TrEMBL
Match: A0A6J1DVS4 (uncharacterized protein LOC111023555 OS=Momordica charantia OX=3673 GN=LOC111023555 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 7.3e-12
Identity = 40/98 (40.82%), Postives = 56/98 (57.14%), Query Frame = 0

Query: 5   CMTVEMAKVLGAVVGVVEKLRVRGRMGGLAYNEGEGSSEYE---------------EPML 64
           CMT +MAK LGA +G VE              E +G S Y+               +P+ 
Sbjct: 136 CMTKDMAKFLGARLGEVE--------------EVDGMSSYDWVRPFLWVRVKINVLKPLR 195

Query: 65  RGVKIKTKEGKEVWCPIQYEKLPDMCFKCGIIGHSYRE 88
           RG+K+KT +GK++WCP++YE+LPD C+ CG +GHS RE
Sbjct: 196 RGLKVKTSDGKDIWCPLRYERLPDFCYGCGCVGHSIRE 219

BLAST of Lag0023023 vs. ExPASy TrEMBL
Match: A0A2N9IXX1 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57290 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.6e-11
Identity = 43/119 (36.13%), Postives = 66/119 (55.46%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKL----RVRGRMGGLAYNEGEGSSEYEEPMLRGVKIKT 60
           +PV  M+ E A  LG  VGVVE++      RGR G +         +  +P+ RG K   
Sbjct: 127 LPVRRMSHEYASALGKAVGVVEQVAEDEEERGREGCMRIRV---KIDISKPLCRGRKATL 186

Query: 61  KEGKEVWCPIQYEKLPDMCFKCGIIGHSYRECQ---ATRGGGEGEDRPYGEWMRAIPSR 113
             GKE+W P +YE+LP+ C+ CG++ H  R+C+    ++G    E++ YG W+RA+  R
Sbjct: 187 TGGKEIWIPFKYERLPNFCYWCGMLTHGDRDCERWLRSKGTLRREEQQYGAWLRAVVDR 242

BLAST of Lag0023023 vs. ExPASy TrEMBL
Match: A0A2N9GDF4 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25281 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.8e-11
Identity = 44/119 (36.97%), Postives = 63/119 (52.94%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKL----RVRGRMGGLAYNEGEGSSEYEEPMLRGVKIKT 60
           +PV  M  E A  LG  +GVVEK+      RGR G +         +  +P+ RG K + 
Sbjct: 127 LPVRRMNHETASALGGTLGVVEKVVESEEERGREGCMRVRV---KVDISKPLCRGRKARL 186

Query: 61  KEGKEVWCPIQYEKLPDMCFKCGIIGHSYRECQ---ATRGGGEGEDRPYGEWMRAIPSR 113
             GKE W   +YE+LP+ C+ CG++ H  REC+    ++G    ED+ YG W+RA   R
Sbjct: 187 LAGKETWIAFKYERLPNFCYWCGLLTHGDRECEKWLRSKGSLRREDQQYGAWLRASVER 242

BLAST of Lag0023023 vs. ExPASy TrEMBL
Match: A0A2N9HFR8 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38336 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.8e-11
Identity = 44/119 (36.97%), Postives = 63/119 (52.94%), Query Frame = 0

Query: 1   MPVNCMTVEMAKVLGAVVGVVEKL----RVRGRMGGLAYNEGEGSSEYEEPMLRGVKIKT 60
           +PV  M  E A  LG  +GVVEK+      RGR G +         +  +P+ RG K + 
Sbjct: 238 LPVRRMNHETASALGGTLGVVEKVVESEEERGREGCMRVRV---KVDISKPLCRGRKARL 297

Query: 61  KEGKEVWCPIQYEKLPDMCFKCGIIGHSYRECQ---ATRGGGEGEDRPYGEWMRAIPSR 113
             GKE W   +YE+LP+ C+ CG++ H  REC+    ++G    ED+ YG W+RA   R
Sbjct: 298 LAGKETWIAFKYERLPNFCYWCGLLTHGDRECEKWLRSKGSLRREDQQYGAWLRASVER 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149484.18.5e-1536.91uncharacterized protein LOC111017902 [Momordica charantia][more]
XP_022156711.11.5e-1140.82uncharacterized protein LOC111023555 [Momordica charantia][more]
XP_042952130.11.3e-1037.50uncharacterized protein At4g02000-like [Carya illinoinensis][more]
XP_015380691.14.8e-1033.85uncharacterized protein LOC107174364 [Citrus sinensis][more]
XP_023913699.16.3e-1036.28uncharacterized protein LOC112025279 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1D7654.1e-1536.91uncharacterized protein LOC111017902 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1DVS47.3e-1240.82uncharacterized protein LOC111023555 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A2N9IXX11.6e-1136.13Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9GDF42.8e-1136.97Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25281 PE=4 SV=1[more]
A0A2N9HFR82.8e-1136.97Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38336 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 46..89
e-value: 2.8E-13
score: 49.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..153
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 75..88
score: 9.059338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0023023.1Lag0023023.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding