Lag0023005 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0023005
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	berberine bridge enzyme-like 26
Location	chr7: 42640586 .. 42643427 (+)
RNA-Seq Expression	Lag0023005
Synteny	Lag0023005

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTCTCCGACGACTTTCTGGTACGTGGACTAGTCTTCTCCTACGTCTTTCCGATATGTGGACGACCGCAAGGTCTGCCATGGCTCGAGTACCTCGCATGTAGAGAGTCCCCATTCGTTGCAGCGTCGCCATCGAAACTGCAGCGGTGGTCCTCTTCACCGTGGGCAATTTCTGCTTCCACATTTCGGCGTACAGACTTCAATCGGCGTCGATTTCTGGGAGAGGAGCCTTGGTGGAGCAGTGAGGTTCATTTTAATGGCGGGGTCGAAGGTGGAGAGAGTGAAGAGAAGAAGAGAAACGAGAGAGTGAAGAGAATAAGAGAAACAAACACTGGTTAGGAGGGCATTTTCGTAAATTTGACGTTTAGGCTACAACTTTTTGCTATTTTTGCAAATTCTTTTGCGGGCTGCTAAATGTGCAAATATTTTGGCCCAATTTGATAGATTTGCAACCGGTCCTAAAATGTTTTGCCAACTCTTGGATCTCATTAATCCATACACCATCTTCTAACAACGTATGTGAACTGCCATTGAGCATAGGTATCAAGCTTTGACAGTCAGACTCAATCACAAGAGATGGAAAAATTGACATAATAATTAATGCAATAAATAAACAAGCAATAACATAATCAATTATCGGTAAAACTCTTCACCTTCGAGATGCCTTCAACTTTTTCGGTGAGGATGTTGATTACCTCAGTGCGTCTGATTCAACTTCAAGAGGCAACGCCCTTTGATTGCAGGTACTTGGAATCGCTTTCAACACCTCCAAAATAGCTTTTGCTTCTAAAACCTTCACTGGCCAATTTCTGTGGATTTTTCGCATTCCGAGGCAGACGAGGGATCCTTCCGAGTCATGCACAACCCAACCCAGGCCTCCCTTTCCGAGCTTGTCGTTCCAGGTCGCATCTGTATTTACTCTCCAGCAGTTCGAGCTTGAATTCTTCTGATGGGCTTGACTCGAGACGCTCCCAATTCTTCTATGAGCTTGACTGGTCAGGTTTGCTTCTTCACTTTCCTTGATGTTGTTCTGAATATCTTGGAAAAATAATTTTGTTGTTGCTCCTTGGTTGTTTGCTACAATTTTGTAGCGAAGAGTTCCATGCCTTTCAAAGTATAATAGCTCCGATTGCTAATTCCTTTGTACTGAGCTTGTCTTCCATCCACCACCAAAAGTCTTTGGTTGTCCAATTTGGCCTGCACACAGAGAACAATTTGTAAGAATTTGGGATAAATTTTGACCACCCCTCTTTAGGGATTTTACATTCCCACATGAGATGGGTTGGAATTTAGGCTCGCTGTTGCTCGATCGACATGGAATTTAGGCATTGTAATCGTTTTTTTAACACAGATGTGCACTGTGTTGCATGAGAGCTTTGAATCTCCCTCATGGCCATGGCGATTTCGAATCGACTGATGCTTGTATCTCGCGGGAAGAGGGGCGTATTTTTTCGGCCCCCAACATCCCGTTTTGGTTTTTCCCTCTTCAAAGAGTAGGTGTAAACCGGTTAACTTTCGTTAATGCATTGATATTTTATTAAAAAAATAACATAATCAATTATCTTAAAAATTAAATTTTACAAAACGTGATTCATGTTAACCCTGGAGACGACAGAAGGTGAGATCTACTCTCTTGTTTCTTATTTGCAGCAGTGTGTCTAAAACTAAATTTCAAATGTATACAAAATTACATTAAAAACAAAAGGTGTTTTTCATTAATAATTATTCCATGTTTGAGAGTGACTTCTAGGTTATCCAGTAATATTTATTGTATTTTATAAATAATTTTATGACACTCATTGTGAAAATTTCGGCTGCTAAATAAAACAGTTGACTATGTTTTCTCTTAAAAGTATCACTGTTTAGCATTTATAAATGATTCCATTCTTGTTTGCCAAAGATCTGCAGAGTTGAGCGAAGAAAGAAAATTGGTAGGAGCTATTTCACTAATTTCATCTCTCTAATTCTTTTATCATTTGCAATGCGGGGTTCTTCAGGCGGAAATGATCTTGACGATTTACTTCGCTGCCTCTCTCTTCATTCCTCTAGCGACGATGATTCAAATTCAGATGATCTCATTCTCACTCCATCTTCATCCTCATACTCTCAAGCTCTAAATTTCACCATTCGAAACCTCAGATTCTCGGGGCCTAATGTCCCAAAGCCAGTCGCCATAATCAAACCATCACATCCTTCCCAAGTCCAGGCGGCCGTCATTTTCTGCAAAAATCACGGCCTCCACATCCGAATTCGGAGCGGCGGCCACGACTTCGAGGGTCTTTCTTACGTCGCCAACGTCCCCTTCGTGTTGATTGATTTGGCCAATCTCAATTCGGTAACCATCAACACCGAAGACAACACTGCGTGGGTTCAATCTGGGGCGAGCTTGGGTGAGCTCTATTACAAACTCGGCCAGAAGACCTCCAGTTTTGGTTTCCCGGCCGGGGTGCAGCCGAATGTGGGCGTTGGTGGGTTCCTTAGCGGCGGTGGGTACGGGCTGATGGTTAGAAAATATGGTCTTGGCGTGGACAATGTGGTGGATGCTCAGCTTGTGGATGCAAATGGAAGGCTTCTGGATAGAGAATCCATGGGGGAGGATTTGTTTTGGGCCATTAGAGGTGGTGGAGGAGGGAGCTTTGGGATTGTTTTGGCGTGGAAGTTGAAGTTGGTTCCAGTTCCTCCCATTGTTACGTCCTTTACGGCTTACAGAAGTTGGGACCAAAACTCCACCAAGTTGGTCCATAGGTGGCAGTACGTTGCCCCCGAGATTGATGAAAGACTGTTCATTGGCATCATGGTGACAGGTTTCTTTGACTTAACTATCCTTCCTAATCATTAG

mRNA sequence

ATGAACTTCTCCGACGACTTTCTGGTACGTGGACTAGTCTTCTCCTACGTCTTTCCGATATGTGGACGACCGCAAGGTCTGCCATGGCTCGAGTACCTCGCATGTAGAGAGTCCCCATTCGTTGCAGCGTCGCCATCGAAACTGCAGCGGTGGTCCTCTTCACCGTGGGCAATTTCTGCTTCCACATTTCGGCGTACAGACTTCAATCGGCGTCGATTTCTGGGAGAGGAGCCTTGGTGGAGCAGTGAGGTTCATTTTAATGGCGGGGTCGAAGGTGGAGAGAGTGAAGAGAAGAAGAGAAACGAGAGAGTGAAGAGAATAAGAGAAACAAACACTGTGCGTCTGATTCAACTTCAAGAGGCAACGCCCTTTGATTGCAGACGAGGGATCCTTCCGAGTCATGCACAACCCAACCCAGGCCTCCCTTTCCGAGCTTGTCGTTCCAGGTCGCATCTGTATTTACTCTCCAGCAGTTCGAGCTTGAATTCTTCTGATGGGCTTGACTCGAGACGCTCCCAATTCTTCTATGAGCTTGACTGCGAAGAGTTCCATGCCTTTCAAAGTATAATAGCTCCGATTGCTAATTCCTTTGTACTGAGCTTGTCTTCCATCCACCACCAAAAGTCTTTGGTTGTCCAATTTGGCCTGCACACAGAGAACAATTTATGTGCACTGTGTTGCATGAGAGCTTTGAATCTCCCTCATGGCCATGGCGATTTCGAATCGACTGATGCTTGTATCTCGCGGGAAGAGGGGCGTATTTTTTCGGCCCCCAACATCCCGTTTTGGTTTTTCCCTCTTCAAAGAGTAGGTGTAAACCGAGTTGAGCGAAGAAAGAAAATTGGTAGGAGCTATTTCACTAATTTCATCTCTCTAATTCTTTTATCATTTGCAATGCGGGGTTCTTCAGGCGGAAATGATCTTGACGATTTACTTCGCTGCCTCTCTCTTCATTCCTCTAGCGACGATGATTCAAATTCAGATGATCTCATTCTCACTCCATCTTCATCCTCATACTCTCAAGCTCTAAATTTCACCATTCGAAACCTCAGATTCTCGGGGCCTAATGTCCCAAAGCCAGTCGCCATAATCAAACCATCACATCCTTCCCAAGTCCAGGCGGCCGTCATTTTCTGCAAAAATCACGGCCTCCACATCCGAATTCGGAGCGGCGGCCACGACTTCGAGGGTCTTTCTTACGTCGCCAACGTCCCCTTCGTGTTGATTGATTTGGCCAATCTCAATTCGGTAACCATCAACACCGAAGACAACACTGCGTGGGTTCAATCTGGGGCGAGCTTGGGTGAGCTCTATTACAAACTCGGCCAGAAGACCTCCAGTTTTGGTTTCCCGGCCGGGGTGCAGCCGAATGTGGGCGTTGGTGGGTTCCTTAGCGGCGGTGGGTACGGGCTGATGGTTAGAAAATATGGTCTTGGCGTGGACAATGTGGTGGATGCTCAGCTTGTGGATGCAAATGGAAGGCTTCTGGATAGAGAATCCATGGGGGAGGATTTGTTTTGGGCCATTAGAGGTGGTGGAGGAGGGAGCTTTGGGATTGTTTTGGCGTGGAAGTTGAAGTTGGTTCCAGTTCCTCCCATTGTTACGTCCTTTACGGCTTACAGAAGTTGGGACCAAAACTCCACCAAGTTGGTCCATAGGTGGCAGTACGTTGCCCCCGAGATTGATGAAAGACTGTTCATTGGCATCATGGTGACAGGTTTCTTTGACTTAACTATCCTTCCTAATCATTAG

Coding sequence (CDS)

ATGAACTTCTCCGACGACTTTCTGGTACGTGGACTAGTCTTCTCCTACGTCTTTCCGATATGTGGACGACCGCAAGGTCTGCCATGGCTCGAGTACCTCGCATGTAGAGAGTCCCCATTCGTTGCAGCGTCGCCATCGAAACTGCAGCGGTGGTCCTCTTCACCGTGGGCAATTTCTGCTTCCACATTTCGGCGTACAGACTTCAATCGGCGTCGATTTCTGGGAGAGGAGCCTTGGTGGAGCAGTGAGGTTCATTTTAATGGCGGGGTCGAAGGTGGAGAGAGTGAAGAGAAGAAGAGAAACGAGAGAGTGAAGAGAATAAGAGAAACAAACACTGTGCGTCTGATTCAACTTCAAGAGGCAACGCCCTTTGATTGCAGACGAGGGATCCTTCCGAGTCATGCACAACCCAACCCAGGCCTCCCTTTCCGAGCTTGTCGTTCCAGGTCGCATCTGTATTTACTCTCCAGCAGTTCGAGCTTGAATTCTTCTGATGGGCTTGACTCGAGACGCTCCCAATTCTTCTATGAGCTTGACTGCGAAGAGTTCCATGCCTTTCAAAGTATAATAGCTCCGATTGCTAATTCCTTTGTACTGAGCTTGTCTTCCATCCACCACCAAAAGTCTTTGGTTGTCCAATTTGGCCTGCACACAGAGAACAATTTATGTGCACTGTGTTGCATGAGAGCTTTGAATCTCCCTCATGGCCATGGCGATTTCGAATCGACTGATGCTTGTATCTCGCGGGAAGAGGGGCGTATTTTTTCGGCCCCCAACATCCCGTTTTGGTTTTTCCCTCTTCAAAGAGTAGGTGTAAACCGAGTTGAGCGAAGAAAGAAAATTGGTAGGAGCTATTTCACTAATTTCATCTCTCTAATTCTTTTATCATTTGCAATGCGGGGTTCTTCAGGCGGAAATGATCTTGACGATTTACTTCGCTGCCTCTCTCTTCATTCCTCTAGCGACGATGATTCAAATTCAGATGATCTCATTCTCACTCCATCTTCATCCTCATACTCTCAAGCTCTAAATTTCACCATTCGAAACCTCAGATTCTCGGGGCCTAATGTCCCAAAGCCAGTCGCCATAATCAAACCATCACATCCTTCCCAAGTCCAGGCGGCCGTCATTTTCTGCAAAAATCACGGCCTCCACATCCGAATTCGGAGCGGCGGCCACGACTTCGAGGGTCTTTCTTACGTCGCCAACGTCCCCTTCGTGTTGATTGATTTGGCCAATCTCAATTCGGTAACCATCAACACCGAAGACAACACTGCGTGGGTTCAATCTGGGGCGAGCTTGGGTGAGCTCTATTACAAACTCGGCCAGAAGACCTCCAGTTTTGGTTTCCCGGCCGGGGTGCAGCCGAATGTGGGCGTTGGTGGGTTCCTTAGCGGCGGTGGGTACGGGCTGATGGTTAGAAAATATGGTCTTGGCGTGGACAATGTGGTGGATGCTCAGCTTGTGGATGCAAATGGAAGGCTTCTGGATAGAGAATCCATGGGGGAGGATTTGTTTTGGGCCATTAGAGGTGGTGGAGGAGGGAGCTTTGGGATTGTTTTGGCGTGGAAGTTGAAGTTGGTTCCAGTTCCTCCCATTGTTACGTCCTTTACGGCTTACAGAAGTTGGGACCAAAACTCCACCAAGTTGGTCCATAGGTGGCAGTACGTTGCCCCCGAGATTGATGAAAGACTGTTCATTGGCATCATGGTGACAGGTTTCTTTGACTTAACTATCCTTCCTAATCATTAG

Protein sequence

MNFSDDFLVRGLVFSYVFPICGRPQGLPWLEYLACRESPFVAASPSKLQRWSSSPWAISASTFRRTDFNRRRFLGEEPWWSSEVHFNGGVEGGESEEKKRNERVKRIRETNTVRLIQLQEATPFDCRRGILPSHAQPNPGLPFRACRSRSHLYLLSSSSSLNSSDGLDSRRSQFFYELDCEEFHAFQSIIAPIANSFVLSLSSIHHQKSLVVQFGLHTENNLCALCCMRALNLPHGHGDFESTDACISREEGRIFSAPNIPFWFFPLQRVGVNRVERRKKIGRSYFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVTGFFDLTILPNH

Homology

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BLAST of Lag0023005 vs. NCBI nr
Match: KAG6607170.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 443.0 bits (1138), Expect = 4.1e-120
Identity = 224/293 (76.45%), Postives = 251/293 (85.67%), Query Frame = 0


Query: 280 KIGRSYFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSY 339
           KIGR+Y  NFIS +L+S  + G  G N L+D L C+S +SS      +  L+ T SSSSY
Sbjct: 2   KIGRNY-VNFIS-VLVSLLLWGCWGSN-LEDFLHCISSNSS------NSTLLYTQSSSSY 61

Query: 340 SQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLS 399
           S  LNFTIRNLRFSGPNVPKP+AIIKPS PSQVQAAVI C NH L IR+RSGGHDFEGLS
Sbjct: 62  SSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQVQAAVICCNNHDLRIRVRSGGHDFEGLS 121

Query: 400 YVANVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVG 459
           YVANV FV+IDL NLNSVT++TE+NTAWVQSGA+ GE+YYKLGQKTS+FGFP GVQP VG
Sbjct: 122 YVANVTFVVIDLVNLNSVTVDTENNTAWVQSGATQGEVYYKLGQKTSAFGFPGGVQPTVG 181

Query: 460 VGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGI 519
           +GGFLSGGGYG+MVR+YGLG DNVVDA LVDANGRLLDR+SMGEDLFWAIRGGGGGSFGI
Sbjct: 182 IGGFLSGGGYGMMVRRYGLGADNVVDALLVDANGRLLDRDSMGEDLFWAIRGGGGGSFGI 241

Query: 520 VLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 573
           VLAWKLKLVPVPP VTSF AYRS DQN+T LVHRWQ+VAP IDERLFIG++V+
Sbjct: 242 VLAWKLKLVPVPPTVTSFAAYRSLDQNATDLVHRWQFVAPVIDERLFIGVIVS 285

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BLAST of Lag0023005 vs. NCBI nr
Match: XP_023525672.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.7 bits (1127), Expect = 7.7e-119
Identity = 222/294 (75.51%), Postives = 250/294 (85.03%), Query Frame = 0


Query: 280 KIGRSYFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSY 339
           +IGR+Y  NFIS +L+S  + G  G N L+D L C+S +SS      +  L+ T SSSSY
Sbjct: 2   EIGRNY-VNFIS-VLVSLLLWGCWGSN-LEDFLHCISSNSS------NSTLLYTQSSSSY 61

Query: 340 SQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLS 399
           S  LNFTIRNLRFSGPNVPKP+AIIKPS PSQVQAAVI C NH L IR+RSGGHDFEGLS
Sbjct: 62  SSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQVQAAVICCNNHDLRIRVRSGGHDFEGLS 121

Query: 400 YVANVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVG 459
           YVANV FV+IDL NLNSVT++ E+NTAWVQSGA+ GE+YYKLG K+S+FGFP GVQP VG
Sbjct: 122 YVANVTFVVIDLVNLNSVTVDIENNTAWVQSGATQGEVYYKLGLKSSAFGFPGGVQPTVG 181

Query: 460 VGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGI 519
           +GGFLSGGGYG+MVRKYGLG DNVVDA LVDANGRLLDR+SMGEDLFWAIRGGGGGSFGI
Sbjct: 182 IGGFLSGGGYGMMVRKYGLGADNVVDALLVDANGRLLDRDSMGEDLFWAIRGGGGGSFGI 241

Query: 520 VLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVTG 574
           VLAWKLKLVPVPP VTSF AYRS DQN+T LVHRWQ+VAP IDERLFIG++V+G
Sbjct: 242 VLAWKLKLVPVPPTVTSFAAYRSLDQNATDLVHRWQFVAPVIDERLFIGVIVSG 286

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BLAST of Lag0023005 vs. NCBI nr
Match: XP_022964765.1 (berberine bridge enzyme-like 26 [Cucurbita moschata])

HSP 1 Score: 433.3 bits (1113), Expect = 3.2e-117
Identity = 221/288 (76.74%), Postives = 248/288 (86.11%), Query Frame = 0


Query: 288 NFISLI--LLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNF 347
           N+++L+  LL FA+  S GGN LDD LRC+     S+DDSN   LI TPSSS+YS  LNF
Sbjct: 6   NYLNLVTLLLFFAISDSFGGN-LDDFLRCV-----SNDDSN-PILINTPSSSTYSSVLNF 65

Query: 348 TIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVAN-V 407
           T+RNLRFSGP+ PKP AII PS  S VQ  VI  KNHG+HIR+RSGGHDFEGLSYVA+ V
Sbjct: 66  TVRNLRFSGPDTPKPFAIINPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGV 125

Query: 408 PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFL 467
           PFVLID+A LNSV+++T+DNTAWV+SGAS+GELYYKLG +TS FGFP GVQPNVGVGGFL
Sbjct: 126 PFVLIDMAKLNSVSVDTDDNTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFL 185

Query: 468 SGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWK 527
           SGGGYGLMVRKYGL VDNVVDAQ+VDANGRL+DR+SMGEDLFWAIRGGGGGSFGIV+AWK
Sbjct: 186 SGGGYGLMVRKYGLAVDNVVDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWK 245

Query: 528 LKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 573
           LKLVPVP IVTSFTA+R WDQNST LVH+WQ VAPEIDERLFIG MVT
Sbjct: 246 LKLVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVAPEIDERLFIGAMVT 286

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BLAST of Lag0023005 vs. NCBI nr
Match: XP_023521064.1 (berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422.2 bits (1084), Expect = 7.5e-114
Identity = 211/267 (79.03%), Postives = 234/267 (87.64%), Query Frame = 0


Query: 307 DLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNFTIRNLRFSGPNVPKPVAIIKP 366
           +LDD LRC+     S+DDSN   LI TPSSS+YS  LNFT+RNLRFSGPN PKP AII P
Sbjct: 3   NLDDFLRCV-----SNDDSN-PILINTPSSSTYSSVLNFTVRNLRFSGPNTPKPCAIINP 62

Query: 367 SHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVAN-VPFVLIDLANLNSVTINTEDNT 426
           S  S VQA VI  KNHG+HIR+RSGGHDFEGLSYVA+ V FVLID+A LNSV+++T+DNT
Sbjct: 63  SQASHVQAGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVAFVLIDMAKLNSVSVDTDDNT 122

Query: 427 AWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFLSGGGYGLMVRKYGLGVDNVVD 486
           AWV+SGAS+GELYYKLG +TS FGFP GVQPNVGVGGFLSGGGYGLMVRKYGL VDNVVD
Sbjct: 123 AWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVD 182

Query: 487 AQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQ 546
           AQ+VDANGR++DR+SMGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP IVTSFTA+R WDQ
Sbjct: 183 AQVVDANGRVVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIWDQ 242

Query: 547 NSTKLVHRWQYVAPEIDERLFIGIMVT 573
           NST LVH+WQ VAPEIDERLFIG MVT
Sbjct: 243 NSTNLVHKWQLVAPEIDERLFIGAMVT 263

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BLAST of Lag0023005 vs. NCBI nr
Match: XP_038894010.1 (berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 417.2 bits (1071), Expect = 2.4e-112
Identity = 205/289 (70.93%), Postives = 240/289 (83.04%), Query Frame = 0


Query: 284 SYFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQAL 343
           SY++    L+LLS A  G  G N  +D + C+SLHSS+++DSN+  LI TPSSSSYS  L
Sbjct: 7   SYYSFISLLLLLSLATLGCWGDNG-EDFVHCISLHSSNNNDSNT-TLIHTPSSSSYSYVL 66

Query: 344 NFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVAN 403
           NF+IRNLRFS P  PKPVAII PSH S VQAAVI CK+HGL IR RSGGHDFEG SYVAN
Sbjct: 67  NFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGRSYVAN 126

Query: 404 VPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGF 463
           VPFVLIDL NLNS+TI+TED +AWVQSGA++G+LYY++ +K+ + GFPAG  P +GVGG+
Sbjct: 127 VPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTIGVGGY 186

Query: 464 LSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAW 523
           LSGGG+G+MVRKYGL VDNVVDA +VDANGR+ DR SMGEDLFWAIRGGGGGSFGIVLAW
Sbjct: 187 LSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFGIVLAW 246

Query: 524 KLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 573
           KLKLVPVPPIVTSF  +++WDQN+  L+HRWQYVAPE+DE LFI   VT
Sbjct: 247 KLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVT 293

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BLAST of Lag0023005 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.7e-79
Identity = 144/287 (50.17%), Postives = 205/287 (71.43%), Query Frame = 0


Query: 285 YFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALN 344
           +FT   S +LLS     S+  ++    L+CLSL    +D +    +I TP+ +S+S  L 
Sbjct: 13  FFTT--STLLLSIPHPVSANRSNQSSFLQCLSL--QLNDSNIVSKVIHTPNDTSFSSVLA 72

Query: 345 FTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANV 404
            +I+N RFS P+VPKPV I+ P  PS VQ+AV   +  G+HIR RSGGHD+EGLSYV + 
Sbjct: 73  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 132

Query: 405 PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFL 464
           PFV++DL NL S+T++ ++ + WVQ+GA++GELYY++G+K  +  FPAGV P VGVGG  
Sbjct: 133 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 192

Query: 465 SGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWK 524
           SGGGYG ++RK+GL  D+V+DA++VDA GR+L+R  MGED FWAIRGGGG SF +VL+WK
Sbjct: 193 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 252

Query: 525 LKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
           + L+ VP  VT F   +  +Q++ K++HRWQ+VA ++ + LFI +M+
Sbjct: 253 IGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML 295

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BLAST of Lag0023005 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.4e-75
Identity = 144/283 (50.88%), Postives = 201/283 (71.02%), Query Frame = 0


Query: 289 FISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNFTIR 348
           F  L+LLS     SS G+  +  L C+S   S   D +  +++  PS+SSY   L  TI+
Sbjct: 12  FCFLLLLS-----SSHGSIPEAFLNCISNKFSL--DVSILNILHVPSNSSYDSVLKSTIQ 71

Query: 349 NLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANVPFVL 408
           N RF     PKP+AII P   S VQ+AVI  K  GL IRIRSGG D+EGLSY + VPF+L
Sbjct: 72  NPRFL--KSPKPLAIITPVLHSHVQSAVICTKQAGLQIRIRSGGADYEGLSYRSEVPFIL 131

Query: 409 IDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFLSGGG 468
           +DL NL S++++ EDN+AWV+SGA++GE Y+++ Q +    FPAGV  +VG+GG LS GG
Sbjct: 132 LDLQNLRSISVDIEDNSAWVESGATIGEFYHEIAQNSPVHAFPAGVSSSVGIGGHLSSGG 191

Query: 469 YGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWKLKLV 528
           +G ++RKYGL  DN++DA++VDA GR+LDRESMGEDLFWAIRGGGG SFG++++WK+KLV
Sbjct: 192 FGTLLRKYGLAADNIIDAKIVDARGRILDRESMGEDLFWAIRGGGGASFGVIVSWKVKLV 251

Query: 529 PVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
            VPP+VT F   +++++    L+H+WQY+  ++ E LF+ + +
Sbjct: 252 KVPPMVTVFILSKTYEEGGLDLLHKWQYIEHKLPEDLFLAVSI 285

[Close]

BLAST of Lag0023005 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 9.3e-75
Identity = 137/284 (48.24%), Postives = 206/284 (72.54%), Query Frame = 0


Query: 290 ISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNFTIRN 349
           ++L+LLS +    S      D + CL  +S +    +S       ++SS+  AL  + +N
Sbjct: 14  VTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQN 73

Query: 350 LRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANV--PFV 409
           LR+  P+ PKP  I +P + + VQAAV+  K   LH+R+RSGGHD+EGLSYV+ +   FV
Sbjct: 74  LRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFV 133

Query: 410 LIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFLSGG 469
           ++DL+ L  ++++ E N+AWV +GAS+GE+YY++ +K+   GFPAG+  ++G+GG + GG
Sbjct: 134 IVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGG 193

Query: 470 GYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWKLKL 529
            YG M+RK+GLG DNV+DA++VDA+G++L+R +MGED+FWAIRGGGGGSFG++LAWK+KL
Sbjct: 194 AYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 253

Query: 530 VPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
           VPVP IVT FT  R+ +Q+ TKL+++WQ VA ++DE LFI +++
Sbjct: 254 VPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVII 297

[Close]

BLAST of Lag0023005 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 9.3e-75
Identity = 133/241 (55.19%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 333 TPSSSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGG 392
           T + S +SQ L  T +NLRF   ++PKP  I  P H S VQA++I  K   +H+R+RSGG
Sbjct: 57  TKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGG 116

Query: 393 HDFEGLSYVANV--PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGF 452
           HD+EGLSYV+ +  PF+L+DL+ +  V IN +DN+AWVQSGA++GELYY++ +K+   GF
Sbjct: 117 HDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGF 176

Query: 453 PAGVQPNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIR 512
           PAG+  ++G+GG ++GG YG M+RKYGLG DNV+DA++VDANG+LLDR +MGED FWAIR
Sbjct: 177 PAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIR 236

Query: 513 GGGGGSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQN-STKLVHRWQYVAPEIDERLFIGI 571
           GG GGSFGI+LAWK+KLVPVP  VT FT  ++  Q+   K++ +WQ VA ++ E LFI +
Sbjct: 237 GGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRV 296

[Close]

BLAST of Lag0023005 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.6e-74
Identity = 140/297 (47.14%), Postives = 203/297 (68.35%), Query Frame = 0


Query: 280 KIGRSYFTNFISLILLSFAMRG----SSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPS 339
           +I R   + FI  +LL+ ++      S+   + ++ L+CLS H  ++DDS    +I T  
Sbjct: 2   EILRFLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLS-HRINEDDSR---IIHTSK 61

Query: 340 SSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDF 399
             SY   LN +I+N RF     PKPV+II P   + VQ+ +   + HG+HIR RSGGHD+
Sbjct: 62  DPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDY 121

Query: 400 EGLSYVA-NVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGV 459
           EGLSY+A + PFV+IDL NL S+T++ ++ T WVQSGA++GELYY++G+ + S  FPAG+
Sbjct: 122 EGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGL 181

Query: 460 QPNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGG 519
            P VG+GG   GGGYG ++RKYGL  DNV+DA +VDANG  LDR+ MGED FWAIRGGGG
Sbjct: 182 YPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGG 241

Query: 520 GSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
            SF +VL+WK++L+ VP +VT F   ++ ++ +  ++++WQY+A ++   LFI  M+
Sbjct: 242 SSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML 294

[Close]

BLAST of Lag0023005 vs. ExPASy TrEMBL
Match: A0A6J1HLR7 (berberine bridge enzyme-like 26 OS=Cucurbita moschata OX=3662 GN=LOC111464766 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 1.6e-117
Identity = 221/288 (76.74%), Postives = 248/288 (86.11%), Query Frame = 0


Query: 288 NFISLI--LLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNF 347
           N+++L+  LL FA+  S GGN LDD LRC+     S+DDSN   LI TPSSS+YS  LNF
Sbjct: 6   NYLNLVTLLLFFAISDSFGGN-LDDFLRCV-----SNDDSN-PILINTPSSSTYSSVLNF 65

Query: 348 TIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVAN-V 407
           T+RNLRFSGP+ PKP AII PS  S VQ  VI  KNHG+HIR+RSGGHDFEGLSYVA+ V
Sbjct: 66  TVRNLRFSGPDTPKPFAIINPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGV 125

Query: 408 PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFL 467
           PFVLID+A LNSV+++T+DNTAWV+SGAS+GELYYKLG +TS FGFP GVQPNVGVGGFL
Sbjct: 126 PFVLIDMAKLNSVSVDTDDNTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFL 185

Query: 468 SGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWK 527
           SGGGYGLMVRKYGL VDNVVDAQ+VDANGRL+DR+SMGEDLFWAIRGGGGGSFGIV+AWK
Sbjct: 186 SGGGYGLMVRKYGLAVDNVVDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWK 245

Query: 528 LKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 573
           LKLVPVP IVTSFTA+R WDQNST LVH+WQ VAPEIDERLFIG MVT
Sbjct: 246 LKLVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVAPEIDERLFIGAMVT 286

[Close]

BLAST of Lag0023005 vs. ExPASy TrEMBL
Match: A0A6J1DHA5 (berberine bridge enzyme-like 27 OS=Momordica charantia OX=3673 GN=LOC111020467 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 8.6e-108
Identity = 202/288 (70.14%), Postives = 237/288 (82.29%), Query Frame = 0


Query: 286 FTNFISLILLSFA-MRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALN 345
           +++FISL+LL++A +  SS GN+L+D L C+SLHSS+ +DSNS  LI TPSS SYS  LN
Sbjct: 7   YSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSST-NDSNS-ILIHTPSSPSYSSILN 66

Query: 346 FTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANV 405
           F IRN RF G +  KPVAII PS  S VQA+VI CKNHG  IR RSGGHDFEGLSYV++V
Sbjct: 67  FNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSV 126

Query: 406 PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFL 465
           PFVLIDL NLNSVT++T+D+TAWVQSGA++GELYY++G+KT + GFP  V PNVGVGGFL
Sbjct: 127 PFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFL 186

Query: 466 SGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWK 525
           SGGG+G MVRKYGL  DNVVDA  VDANG+L DR+SMG+DLFWAIRGGGGGSFGIVLAWK
Sbjct: 187 SGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWK 246

Query: 526 LKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 573
           LKLVPVP IVT     ++WDQ+  KLVH+WQYVAPE DE LFI + VT
Sbjct: 247 LKLVPVPAIVTCIAINKTWDQSVAKLVHQWQYVAPEADENLFISVTVT 292

[Close]

BLAST of Lag0023005 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 3.6e-106
Identity = 199/297 (67.00%), Postives = 237/297 (79.80%), Query Frame = 0


Query: 276 ERRKKIGRSYFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPS 335
           E   KI  S F  F+SL+ +SFA  G  G ++L D L C+SLHSS+ + +++  LI TPS
Sbjct: 5   EIEMKIFLSNF-GFVSLVFVSFASWG-CGSDNLQDFLHCISLHSSNHNSNST--LIHTPS 64

Query: 336 SSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDF 395
           SSSY   LNF+IRNLRF+  + PKPVAI+ P   SQVQA VI CK HGL IR RSGG DF
Sbjct: 65  SSSYLYVLNFSIRNLRFAESDTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGQDF 124

Query: 396 EGLSYVANVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQ 455
           EG SYV+NVPFVLIDL NLNS+TI+TED TAW QSGA++GELYYK+GQK+ +  FPAG+ 
Sbjct: 125 EGRSYVSNVPFVLIDLINLNSITIDTEDETAWAQSGATVGELYYKIGQKSRTLAFPAGLV 184

Query: 456 PNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGG 515
            N+G+GGF SGGG+G+++RKYGL VDNVVDAQLVDANG+LLDR+SMGEDLFWAIRGGGGG
Sbjct: 185 ANLGLGGFFSGGGFGMLLRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGG 244

Query: 516 SFGIVLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 573
           SFGIVL WKLKLV VPP VTSF  ++SWD N+ KL+H+WQYVAPE+DE LFI   VT
Sbjct: 245 SFGIVLTWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHQWQYVAPEVDEDLFITAWVT 297

[Close]

BLAST of Lag0023005 vs. ExPASy TrEMBL
Match: A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 2.3e-105
Identity = 199/305 (65.25%), Postives = 240/305 (78.69%), Query Frame = 0


Query: 271 GVNRVERRKKIGRSYFTN---FISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNS 330
           G+N +E   KI    ++N   FISL+LLSFA     G ++L D L C+SLHSS+   +++
Sbjct: 11  GLN-IEIADKIDLQPYSNIEDFISLLLLSFA-SWVCGSDNLQDFLHCISLHSSNHHSNST 70

Query: 331 DDLILTPSSSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIR 390
             LI TPSSSSY   LNF+IRNLRF+  + PKPVAI+ P  PS+VQA VI CK HGL IR
Sbjct: 71  --LIHTPSSSSYPHVLNFSIRNLRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIR 130

Query: 391 IRSGGHDFEGLSYVANVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSS 450
            RSGGHDFEG SYV+NVPFVLIDL NLNS+TI+ ++ TAW QSGA++GELYYK+G+K+ +
Sbjct: 131 TRSGGHDFEGRSYVSNVPFVLIDLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRT 190

Query: 451 FGFPAGVQPNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFW 510
             FPAG   ++G+GGF SGGG G++VRKYGL VDNVVDA LVDANG+LLDR+SMGEDLFW
Sbjct: 191 LAFPAGFTASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFW 250

Query: 511 AIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFI 570
           AIRGGGGGSFGIVLAWKLKLV VPP VTSF  ++SWD N+ KL+HRWQYVAP++DE LFI
Sbjct: 251 AIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFI 310

Query: 571 GIMVT 573
              VT
Sbjct: 311 TAWVT 311

[Close]

BLAST of Lag0023005 vs. ExPASy TrEMBL
Match: A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 4.0e-105
Identity = 197/292 (67.47%), Postives = 234/292 (80.14%), Query Frame = 0


Query: 276 ERRKKIGRSYFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPS 335
           E   KI  S F +FISL+ +SFA  G  G ++L D L C+SLHSS+ + +++  LI TPS
Sbjct: 5   EIEMKIFLSNF-SFISLVFVSFASWG-CGSDNLQDFLHCISLHSSNHNSNST--LIHTPS 64

Query: 336 SSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDF 395
           SSSY   LNF+IRNLRF+  + PKPVAI+ P   S+VQ  VI CK HGL IR RSGG DF
Sbjct: 65  SSSYLYVLNFSIRNLRFAESDTPKPVAIVTPWQASEVQTTVICCKTHGLQIRTRSGGQDF 124

Query: 396 EGLSYVANVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQ 455
           EG SYV+NVPFVLIDL NLNS+TI+TED TAW QSGA++GELYYK+G+K+ +  FPAG+ 
Sbjct: 125 EGRSYVSNVPFVLIDLTNLNSITIDTEDETAWAQSGATVGELYYKIGEKSRTLAFPAGLV 184

Query: 456 PNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGG 515
            N+G+GGF SGGG+G++VRKYGL VDNVVDAQLVDANG+LLDR+SMGEDLFWAIRGGGGG
Sbjct: 185 ANLGLGGFFSGGGFGMLVRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGG 244

Query: 516 SFGIVLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFI 568
           SFGIVL WKLKLV VPP VTSF  ++SWD N+ KL+HRWQYVAPE DE LFI
Sbjct: 245 SFGIVLTWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPEADEDLFI 292

[Close]

BLAST of Lag0023005 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 297.0 bits (759), Expect = 3.4e-80
Identity = 144/287 (50.17%), Postives = 205/287 (71.43%), Query Frame = 0


Query: 285 YFTNFISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALN 344
           +FT   S +LLS     S+  ++    L+CLSL    +D +    +I TP+ +S+S  L 
Sbjct: 13  FFTT--STLLLSIPHPVSANRSNQSSFLQCLSL--QLNDSNIVSKVIHTPNDTSFSSVLA 72

Query: 345 FTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANV 404
            +I+N RFS P+VPKPV I+ P  PS VQ+AV   +  G+HIR RSGGHD+EGLSYV + 
Sbjct: 73  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 132

Query: 405 PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFL 464
           PFV++DL NL S+T++ ++ + WVQ+GA++GELYY++G+K  +  FPAGV P VGVGG  
Sbjct: 133 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 192

Query: 465 SGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWK 524
           SGGGYG ++RK+GL  D+V+DA++VDA GR+L+R  MGED FWAIRGGGG SF +VL+WK
Sbjct: 193 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 252

Query: 525 LKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
           + L+ VP  VT F   +  +Q++ K++HRWQ+VA ++ + LFI +M+
Sbjct: 253 IGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML 295

[Close]

BLAST of Lag0023005 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 282.7 bits (722), Expect = 6.6e-76
Identity = 137/284 (48.24%), Postives = 206/284 (72.54%), Query Frame = 0


Query: 290 ISLILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNFTIRN 349
           ++L+LLS +    S      D + CL  +S +    +S       ++SS+  AL  + +N
Sbjct: 14  VTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQN 73

Query: 350 LRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYVANV--PFV 409
           LR+  P+ PKP  I +P + + VQAAV+  K   LH+R+RSGGHD+EGLSYV+ +   FV
Sbjct: 74  LRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFV 133

Query: 410 LIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFLSGG 469
           ++DL+ L  ++++ E N+AWV +GAS+GE+YY++ +K+   GFPAG+  ++G+GG + GG
Sbjct: 134 IVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGG 193

Query: 470 GYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWKLKL 529
            YG M+RK+GLG DNV+DA++VDA+G++L+R +MGED+FWAIRGGGGGSFG++LAWK+KL
Sbjct: 194 AYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 253

Query: 530 VPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
           VPVP IVT FT  R+ +Q+ TKL+++WQ VA ++DE LFI +++
Sbjct: 254 VPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVII 297

[Close]

BLAST of Lag0023005 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 282.7 bits (722), Expect = 6.6e-76
Identity = 133/241 (55.19%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 333 TPSSSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGG 392
           T + S +SQ L  T +NLRF   ++PKP  I  P H S VQA++I  K   +H+R+RSGG
Sbjct: 57  TKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGG 116

Query: 393 HDFEGLSYVANV--PFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGF 452
           HD+EGLSYV+ +  PF+L+DL+ +  V IN +DN+AWVQSGA++GELYY++ +K+   GF
Sbjct: 117 HDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGF 176

Query: 453 PAGVQPNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIR 512
           PAG+  ++G+GG ++GG YG M+RKYGLG DNV+DA++VDANG+LLDR +MGED FWAIR
Sbjct: 177 PAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIR 236

Query: 513 GGGGGSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQN-STKLVHRWQYVAPEIDERLFIGI 571
           GG GGSFGI+LAWK+KLVPVP  VT FT  ++  Q+   K++ +WQ VA ++ E LFI +
Sbjct: 237 GGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRV 296

[Close]

BLAST of Lag0023005 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 280.4 bits (716), Expect = 3.3e-75
Identity = 140/297 (47.14%), Postives = 203/297 (68.35%), Query Frame = 0


Query: 280 KIGRSYFTNFISLILLSFAMRG----SSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPS 339
           +I R   + FI  +LL+ ++      S+   + ++ L+CLS H  ++DDS    +I T  
Sbjct: 2   EILRFLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLS-HRINEDDSR---IIHTSK 61

Query: 340 SSSYSQALNFTIRNLRFSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDF 399
             SY   LN +I+N RF     PKPV+II P   + VQ+ +   + HG+HIR RSGGHD+
Sbjct: 62  DPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDY 121

Query: 400 EGLSYVA-NVPFVLIDLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGV 459
           EGLSY+A + PFV+IDL NL S+T++ ++ T WVQSGA++GELYY++G+ + S  FPAG+
Sbjct: 122 EGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGL 181

Query: 460 QPNVGVGGFLSGGGYGLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGG 519
            P VG+GG   GGGYG ++RKYGL  DNV+DA +VDANG  LDR+ MGED FWAIRGGGG
Sbjct: 182 YPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGG 241

Query: 520 GSFGIVLAWKLKLVPVPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
            SF +VL+WK++L+ VP +VT F   ++ ++ +  ++++WQY+A ++   LFI  M+
Sbjct: 242 SSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML 294

[Close]

BLAST of Lag0023005 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 279.3 bits (713), Expect = 7.3e-75
Identity = 139/282 (49.29%), Postives = 192/282 (68.09%), Query Frame = 0


Query: 292 LILLSFAMRGSSGGNDLDDLLRCLSLHSSSDDDSNSDDLILTPSSSSYSQALNFTIRNLR 351
           L  + F  + SS   + +   +CL+  S+SD        I    + SYS  L   IRNLR
Sbjct: 9   LFFVVFIWQSSSSSANSETFTQCLT--SNSDPKHPISPAIFFSGNGSYSSVLQANIRNLR 68

Query: 352 FSGPNVPKPVAIIKPSHPSQVQAAVIFCKNHGLHIRIRSGGHDFEGLSYV--ANVPFVLI 411
           F+  + PKP  II  +H S VQAA+   K H L ++IRSGGHD++GLSYV  +  PF ++
Sbjct: 69  FNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVL 128

Query: 412 DLANLNSVTINTEDNTAWVQSGASLGELYYKLGQKTSSFGFPAGVQPNVGVGGFLSGGGY 471
           D+ NL SV ++    TAWVQ+GA LGE+YY + +K+ +  +PAG+ P VGVGG +SGGGY
Sbjct: 129 DMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGY 188

Query: 472 GLMVRKYGLGVDNVVDAQLVDANGRLLDRESMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 531
           G M+RKYGL VDN +DA++VD NG++LDR+ MGEDL+WAI GGGGGS+G+VLA+K+ LV 
Sbjct: 189 GNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVE 248

Query: 532 VPPIVTSFTAYRSWDQNSTKLVHRWQYVAPEIDERLFIGIMV 572
           VP  VT F   R+ +QN+T ++HRWQ VAP++ + LFI  ++
Sbjct: 249 VPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVI 288

The following BLAST results are available for this feature:

BLAST of Lag0023005 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6607170.1	4.1e-120	76.45	Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023525672.1	7.7e-119	75.51	berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo]	[more]
XP_022964765.1	3.2e-117	76.74	berberine bridge enzyme-like 26 [Cucurbita moschata]	[more]
XP_023521064.1	7.5e-114	79.03	berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo]	[more]
XP_038894010.1	2.4e-112	70.93	berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida]	[more]

BLAST of Lag0023005 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVG5	4.7e-79	50.17	Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=...	[more]
A0A2S1XB67	1.4e-75	50.88	O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1	[more]
Q93ZA3	9.3e-75	48.24	Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=...	[more]
Q9FKU8	9.3e-75	55.19	Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=...	[more]
Q9FI25	4.6e-74	47.14	Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=...	[more]

BLAST of Lag0023005 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HLR7	1.6e-117	76.74	berberine bridge enzyme-like 26 OS=Cucurbita moschata OX=3662 GN=LOC111464766 PE...	[more]
A0A6J1DHA5	8.6e-108	70.14	berberine bridge enzyme-like 27 OS=Momordica charantia OX=3673 GN=LOC111020467 P...	[more]
A0A6J1I038	3.6e-106	67.00	berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3...	[more]
A0A6J1HP64	2.3e-105	65.25	berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE...	[more]
A0A6J1EVN7	4.0e-105	67.47	berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE...	[more]

BLAST of Lag0023005 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20820.1	3.4e-80	50.17	FAD-binding Berberine family protein	[more]
AT1G30760.1	6.6e-76	48.24	FAD-binding Berberine family protein	[more]
AT5G44400.1	6.6e-76	55.19	FAD-binding Berberine family protein	[more]
AT5G44410.1	3.3e-75	47.14	FAD-binding Berberine family protein	[more]
AT1G30700.1	7.3e-75	49.29	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.462.20		coord: 533..570 e-value: 1.5E-71 score: 243.7
None	No IPR available	PANTHER	PTHR32448:SF16	BERBERINE BRIDGE ENZYME-LIKE 17	coord: 289..571
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 289..571
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 305..414 e-value: 5.8E-38 score: 131.0
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 361..496 e-value: 3.8E-30 score: 104.4
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 419..532 e-value: 1.5E-71 score: 243.7
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 356..530 score: 20.840033
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 327..531

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0023005.1	Lag0023005.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding