Lag0022207 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0022207
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	zf-RVT domain-containing protein
Location	chr7: 21179022 .. 21180147 (-)
RNA-Seq Expression	Lag0022207
Synteny	Lag0022207

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCAAAGTGGACAATATCATACCATTGTGGAGATCTATGTCGCGTCCGGTTGTCCCTTATAGTTTCACTGTCAGGAGTGGGTACCGGTTGGCTCTATCAATGGTTACTCAAACACGTCCCTCCTCATCTAACTCTGACCGTATGTGTGTTTGGTGGAAGAGCCTGTGGAAGCTGAATGTTCCAAGCAAGATGAAGTTTTTTCTATGGCGGTTGTTCCATGATCGCTTGCCGACGAAGGTAAATCTTATGAGACGTGATGTTAACATTCTGGGTTTGTGTGTTCTTTGTAATTTTGTTGTGGAGGATTCTCTTCATCTTTTCTGGAATTGCTCGGTTGTTAGAAATATGTGGCTTTGTTCGAAGTTTGCACCTCTCTATCATTCCTAATGTTGTTCATCGTTTGAGGAAATCATGTGGTCAATGAAGGATAGACTGAGTCTGTAGGATTTTGAACTTGTGATTGTCTTTTGGTCGTCCATTTGGAATTTGCGAAACTCTTGGTGCTGGGAGGATTAGTTGATGATAGAGACTTGTGGATATGGTCTCAAGAATACCTTTCTACTTTTCAGGCTGCTGCCAATTGTCGTCTTGCTCGACCTTGTTTGTAGGGGATTTCGGATAGTACAATTCGATGTGGTGGTTGGACGTCGCCAATAGGTGGTGAGTTGAAATTAAATTGTGATGCCTTTGTTAGGCCTGCTATGGGTGAAGCTGGGGGGTTGTTATATGTGATGTCGTTGGTGCAATGTTGATGGTTGCTTGTTGCGTGTTGCCGAAATGTTGGAGTGTGGACATTGCAGAGGGTTGGGTGTTGATTTGAGGCATCGAGGTTGCACAGCAAATGGGGTTTTCTAGCTTTGGTGTGGAGTTGGATTCGTTGAAGTTGATTGATGTGTTGCGCGACGAGGTGACTGCTTTGTCTGAAATTGGGTTCTTGATGGCTGAGGTCCGACGGTTGCTGCAGGGTATCTCAGTGGAGAATTTTTTGTTTACACCACGACAAGGGAATAAGGTGGCTCATGTGTTGGCCAATTTTGCTTTTGTTTACTCTGATTGTGTTTGGCTTGAGGATTGGCCTTGTGAAGTCTCTGGTGTACTGATGGGTGATGCCACCTTAATCTAA

mRNA sequence

ATGCCCAAAGTGGACAATATCATACCATTGTGGAGATCTATGTCGCGTCCGGTTGTCCCTTATAGTTTCACTGTCAGGAGTGGGTACCGGTTGGCTCTATCAATGGTTACTCAAACACGTCCCTCCTCATCTAACTCTGACCGTATGTGTGTTTGGTGGAAGAGCCTGTGGAAGCTGAATGTTCCAAGCAAGATGAAGTTTTTTCTATGGCGGTTGTTCCATGATCGCTTGCCGACGAAGGGGATTTCGGATAGTACAATTCGATGTGGTGGTTGGACGTCGCCAATAGGTGGCATCGAGGTTGCACAGCAAATGGGGTTTTCTAGCTTTGGTGTGGAGTTGGATTCGTTGAAGTTGATTGATGTGTTGCGCGACGAGGTGACTGCTTTGTCTGAAATTGGGTTCTTGATGGCTGAGGTCCGACGGTTGCTGCAGGGTATCTCAGTGGAGAATTTTTTGTTTACACCACGACAAGGGAATAAGGTGGCTCATGTGTTGGCCAATTTTGCTTTTGTTTACTCTGATTGTGTTTGGCTTGAGGATTGGCCTTGTGAAGTCTCTGGTGTACTGATGGGTGATGCCACCTTAATCTAA

Coding sequence (CDS)

ATGCCCAAAGTGGACAATATCATACCATTGTGGAGATCTATGTCGCGTCCGGTTGTCCCTTATAGTTTCACTGTCAGGAGTGGGTACCGGTTGGCTCTATCAATGGTTACTCAAACACGTCCCTCCTCATCTAACTCTGACCGTATGTGTGTTTGGTGGAAGAGCCTGTGGAAGCTGAATGTTCCAAGCAAGATGAAGTTTTTTCTATGGCGGTTGTTCCATGATCGCTTGCCGACGAAGGGGATTTCGGATAGTACAATTCGATGTGGTGGTTGGACGTCGCCAATAGGTGGCATCGAGGTTGCACAGCAAATGGGGTTTTCTAGCTTTGGTGTGGAGTTGGATTCGTTGAAGTTGATTGATGTGTTGCGCGACGAGGTGACTGCTTTGTCTGAAATTGGGTTCTTGATGGCTGAGGTCCGACGGTTGCTGCAGGGTATCTCAGTGGAGAATTTTTTGTTTACACCACGACAAGGGAATAAGGTGGCTCATGTGTTGGCCAATTTTGCTTTTGTTTACTCTGATTGTGTTTGGCTTGAGGATTGGCCTTGTGAAGTCTCTGGTGTACTGATGGGTGATGCCACCTTAATCTAA

Protein sequence

MPKVDNIIPLWRSMSRPVVPYSFTVRSGYRLALSMVTQTRPSSSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPTKGISDSTIRCGGWTSPIGGIEVAQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFLMAEVRRLLQGISVENFLFTPRQGNKVAHVLANFAFVYSDCVWLEDWPCEVSGVLMGDATLI

Homology

[Close]

BLAST of Lag0022207 vs. NCBI nr
Match: KAB2636102.1 (hypothetical protein D8674_026636 [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 78.2 bits (191), Expect = 8.9e-11
Identity = 51/154 (33.12%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 43  SSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPT-----KGISDSTIRCGGWTSP-- 102
           SSN++ + + WK LW  NVP+K+K   W++  D +PT     K  ++S + C    +P  
Sbjct: 2   SSNANSLGLLWKKLWATNVPTKVKICAWKIARDIIPTKMNLRKKRANSDLLCLFCGAPEE 61

Query: 103 --------IGGIEVAQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFLMAEVRRLLQGI 162
                     G  +  Q GF +F +E DSLK++  L D  T LS IG ++ + + LL  +
Sbjct: 62  STPEALVAREGALLGTQRGFQNFILESDSLKIVAALNDPSTNLSIIGHIIEDSKALLASV 121

Query: 163 SVENFLFTPRQGNKVAHVLANFAFV-YSDCVWLE 181
           +        RQ N  AH LA +A +  S+C+W E
Sbjct: 122 TGAISTNVRRQANCCAHRLACYALLSNSNCIWNE 155

[Close]

BLAST of Lag0022207 vs. NCBI nr
Match: XP_042969199.1 (uncharacterized protein LOC122301911 [Carya illinoinensis])

HSP 1 Score: 77.4 bits (189), Expect = 1.5e-10
Identity = 53/217 (24.42%), Postives = 90/217 (41.47%), Query Frame = 0


Query: 22  SFTVRSGYRLALSMVTQTRPSSSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPT-- 81
           SF+V+S Y         +   SS+ +   V+WKSLW L +P KMK F W+   ++LPT  
Sbjct: 156 SFSVKSAYCFLQQNQHLSYWQSSSGNSEAVFWKSLWHLKLPKKMKIFAWKACQEKLPTYL 215

Query: 82  ----KGISDS----------------------TIRCGGWTSPIG------------GIEV 141
               K + D                        + C      +             G+++
Sbjct: 216 NLKKKYVLDDAACVLCNQAGVGVVLRNHNGEVVVACSKVEKEVSFAEFIEAIALLRGLQL 275

Query: 142 AQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFLMAEVRRLLQGISVENFLFTPRQGNK 198
             Q G     ++ D L L++ L +    L++  F++ ++RRL+        +   R GN 
Sbjct: 276 CVQWGVPKLMLKTDCLILVNALNENSECLTDFAFILQDIRRLMAAFQEVKVVHVNRLGNL 335

[Close]

BLAST of Lag0022207 vs. NCBI nr
Match: RXH82922.1 (hypothetical protein DVH24_003420 [Malus domestica])

HSP 1 Score: 77.0 bits (188), Expect = 2.0e-10
Identity = 57/178 (32.02%), Postives = 77/178 (43.26%), Query Frame = 0


Query: 53  WKSLWKLNVPSKMKFFLWRLFHDRLPTK--------GISDSTIRCGG------------- 112
           WK LWK N+P K+K F W   HD LPTK        G+  +   C G             
Sbjct: 42  WKHLWKANIPPKVKNFTWTACHDILPTKVNLHKKGVGVEVACGVCQGEEETVAHVLLTCP 101

Query: 113 ------WTSPIG---------GIEVAQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFL 172
                 ++SP G          ++ AQ  GF    VE DS K+I VL       S +G +
Sbjct: 102 FARAVWFSSPFGLWLSGSIRILLKEAQLQGFQQVVVESDSQKVIAVLNGTYADSSALGMI 161

Query: 173 MAEVRRLLQGISVENFLFTPRQGNKVAHVLANFAFVYS-DCVWLEDWPCEVSGVLMGD 194
             +V +L    S   F+   R  N VAH LA FA   S + VW+E+ P  +  +L+ D
Sbjct: 162 AEDVLQLASNFSRVKFIHASRLCNGVAHHLAKFALSSSNNLVWIEEPPTLIHDLLLQD 219

[Close]

BLAST of Lag0022207 vs. NCBI nr
Match: XP_042952134.1 (uncharacterized protein LOC122289223 [Carya illinoinensis])

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-07
Identity = 52/202 (25.74%), Postives = 88/202 (43.56%), Query Frame = 0


Query: 23  FTVRSGYRLALSMVT-QTRPSSSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPT-- 82
           ++V+S Y+  L M T + R   S++    + WK LWKLN+  +++ F WR   + LPT  
Sbjct: 578 YSVKSAYQFFLFMETDRQRAECSSAADQNLLWKKLWKLNISHRIRVFAWRTCKNILPTLQ 637

Query: 83  -----KGISDSTIRCGGWTSPIG----------------------------------GIE 142
                K I ++  +C    S IG                                  GI+
Sbjct: 638 NLKPRKVIDNANCQCDQCRSGIGVVLRDDKGQVTFATSKPENALTDPMEIELTAILRGIQ 697

Query: 143 VAQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFLMAEVRRLLQGISVENFLFTPRQGN 182
           +   +G     +E+D+L +I+ L+ E  + +    L+ E++ LL  +   +  +  R+ N
Sbjct: 698 LCIPLGIVELQIEIDALVVIEELKKEGRSSTLWSTLIQEIKALLSTLPNWSIQYRGRESN 757

[Close]

BLAST of Lag0022207 vs. NCBI nr
Match: XP_030509135.1 (uncharacterized protein LOC115723805 [Cannabis sativa])

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-07
Identity = 31/58 (53.45%), Postives = 39/58 (67.24%), Query Frame = 0


Query: 22  SFTVRSGYRLALSMVTQTRPSSSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPT 80
           S+TV SGY LA + + Q  PSSS+S     WWK LW L +P K+K FLWR+ +D LPT
Sbjct: 457 SYTVNSGYHLAHTKLHQNEPSSSDSKSSNDWWKKLWALPLPPKLKHFLWRVCYDILPT 514

[Close]

BLAST of Lag0022207 vs. ExPASy TrEMBL
Match: A0A5N5IC60 (Uncharacterized protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_026636 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.3e-11
Identity = 51/154 (33.12%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 43  SSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPT-----KGISDSTIRCGGWTSP-- 102
           SSN++ + + WK LW  NVP+K+K   W++  D +PT     K  ++S + C    +P  
Sbjct: 2   SSNANSLGLLWKKLWATNVPTKVKICAWKIARDIIPTKMNLRKKRANSDLLCLFCGAPEE 61

Query: 103 --------IGGIEVAQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFLMAEVRRLLQGI 162
                     G  +  Q GF +F +E DSLK++  L D  T LS IG ++ + + LL  +
Sbjct: 62  STPEALVAREGALLGTQRGFQNFILESDSLKIVAALNDPSTNLSIIGHIIEDSKALLASV 121

Query: 163 SVENFLFTPRQGNKVAHVLANFAFV-YSDCVWLE 181
           +        RQ N  AH LA +A +  S+C+W E
Sbjct: 122 TGAISTNVRRQANCCAHRLACYALLSNSNCIWNE 155

[Close]

BLAST of Lag0022207 vs. ExPASy TrEMBL
Match: A0A498ILS0 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_003420 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 9.6e-11
Identity = 57/178 (32.02%), Postives = 77/178 (43.26%), Query Frame = 0


Query: 53  WKSLWKLNVPSKMKFFLWRLFHDRLPTK--------GISDSTIRCGG------------- 112
           WK LWK N+P K+K F W   HD LPTK        G+  +   C G             
Sbjct: 42  WKHLWKANIPPKVKNFTWTACHDILPTKVNLHKKGVGVEVACGVCQGEEETVAHVLLTCP 101

Query: 113 ------WTSPIG---------GIEVAQQMGFSSFGVELDSLKLIDVLRDEVTALSEIGFL 172
                 ++SP G          ++ AQ  GF    VE DS K+I VL       S +G +
Sbjct: 102 FARAVWFSSPFGLWLSGSIRILLKEAQLQGFQQVVVESDSQKVIAVLNGTYADSSALGMI 161

Query: 173 MAEVRRLLQGISVENFLFTPRQGNKVAHVLANFAFVYS-DCVWLEDWPCEVSGVLMGD 194
             +V +L    S   F+   R  N VAH LA FA   S + VW+E+ P  +  +L+ D
Sbjct: 162 AEDVLQLASNFSRVKFIHASRLCNGVAHHLAKFALSSSNNLVWIEEPPTLIHDLLLQD 219

[Close]

BLAST of Lag0022207 vs. ExPASy TrEMBL
Match: M5W2B9 (Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa018827mg PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.8e-09
Identity = 56/202 (27.72%), Postives = 90/202 (44.55%), Query Frame = 0


Query: 17  PVVPYSF-----TVRSGYRLALSMVTQTRP-SSSNSDRMCVWWKSLWKLNVPSKMKFFLW 76
           P+ PY F     +V++GYR+ALS   +      S+S     +WK LWKL VPSK+K  +W
Sbjct: 89  PLSPYLFLICKYSVKTGYRVALSQCDENFDIGGSDSSAQQSFWKQLWKLEVPSKIKNLVW 148

Query: 77  RLFHDRLPT------KGISDSTI--RCGGWTSP--------------------------I 136
           R   D L T      + +  S +  +CG +                             +
Sbjct: 149 RACSDILRTAINLYKRKVVQSQVCFKCGKYYEDACHALWSYIMKGFICGFATFGPPELNV 208

Query: 137 GGIEVAQQ---------MGFSSFGVELDSLKLIDVLRDEVTALSEIGFLMAEVRRLLQGI 170
            GIE+             G++ F +  DS +++ +L+  +   S +G ++ ++RRL+   
Sbjct: 209 LGIELVAMREGLLMMGIKGYTRFVLATDSKEVVAMLKGALEWWSNLGNVVKDIRRLMMDC 268

[Close]

BLAST of Lag0022207 vs. ExPASy TrEMBL
Match: A0A2N9IYL5 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57874 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.4e-08
Identity = 59/248 (23.79%), Postives = 89/248 (35.89%), Query Frame = 0


Query: 23   FTVRSGYRLALSMVTQTRPSSSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPTKG- 82
            ++VRSGY+  L+   +  P SS+  RM   WKS+W LNVP K++ FLWR  H+ LPTK  
Sbjct: 1197 YSVRSGYKFLLNESHREDPGSSDPTRMSQLWKSVWSLNVPPKIRHFLWRACHNSLPTKSN 1256

Query: 83   -----------------ISDSTIRC---------------------------GGWTSPIG 142
                              S+STI                               W  P  
Sbjct: 1257 LHHRHILDDPSCTNCSYQSESTIHALWQCKMVQPVWQSISWGSQNLPGSVELVKWNPPAQ 1316

Query: 143  G-------------------------------------------------------IEVA 171
            G                                                       I+ A
Sbjct: 1317 GSYKVNYDGAVFSDFNAAGIGVIVRNHQGEVMGSLSHRIPFPHSVEALEASAARSAIQFA 1376

[Close]

BLAST of Lag0022207 vs. ExPASy TrEMBL
Match: A0A803Q328 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.5e-08
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 22  SFTVRSGYRLALSMVTQTRPSSSNSDRMCVWWKSLWKLNVPSKMKFFLWRLFHDRLPT 80
           S+ V SGY LA   +  T PS SN+     WWK+LW L++P K+K FLWR+ HD LPT
Sbjct: 902 SYAVNSGYHLAHRQLHHTEPSPSNNKSSHDWWKALWNLSLPPKVKHFLWRVCHDILPT 959

The following BLAST results are available for this feature:

BLAST of Lag0022207 vs. NCBI nr


Match Name	E-value	Identity	Description
KAB2636102.1	8.9e-11	33.12	hypothetical protein D8674_026636 [Pyrus ussuriensis x Pyrus communis]	[more]
XP_042969199.1	1.5e-10	24.42	uncharacterized protein LOC122301911 [Carya illinoinensis]	[more]
RXH82922.1	2.0e-10	32.02	hypothetical protein DVH24_003420 [Malus domestica]	[more]
XP_042952134.1	1.2e-07	25.74	uncharacterized protein LOC122289223 [Carya illinoinensis]	[more]
XP_030509135.1	1.2e-07	53.45	uncharacterized protein LOC115723805 [Cannabis sativa]	[more]

BLAST of Lag0022207 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0022207 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5N5IC60	4.3e-11	33.12	Uncharacterized protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D867...	[more]
A0A498ILS0	9.6e-11	32.02	Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_003420 PE=4 SV=1	[more]
M5W2B9	1.8e-09	27.72	Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa018827m...	[more]
A0A2N9IYL5	3.4e-08	23.79	RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57874 ...	[more]
A0A803Q328	4.5e-08	51.72	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 97..170 e-value: 1.5E-9 score: 37.7
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 23..80 e-value: 2.2E-8 score: 34.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0022207.1	Lag0022207.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity