Lag0022172 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0022172
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Bet v 1 related allergen
Location	chr7: 20305468 .. 20306233 (+)
RNA-Seq Expression	Lag0022172
Synteny	Lag0022172

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTACTATTGTTGTGGAAGTTGACCTTGAATTTGAATCGTTCGTCCCCGCAGAGAAGTTTTTCAAGGCCATCTGCGACTTCGACAATCTTCCCAAGGTCGCCCCTCATCTTATTCAGAGCGTTGAAATCATCGAGGAAACCAAGAAGATTAACTTCACCCCAGGTCAAAATAACAAATTCTTTAATTTACAACACCTTTTTGTTATTACTCTCCCTACAAGTTAAACTAACATAACGCTCGACTCAAAGCAACTACTAATTATCAATAATTATTGGAAGAGAAATAACGACACATTAAATTACTCGAGTTTGAAACAGGAAACTTGCTCATGTTAACTACACTCAACTTAAGGCTTAAGCTATATAATTAGTGTATATTGAATAATTATATTCTTAAAATGATTTTGTCACTTATAAATATGAACGAACAAGTTCTTCCATCAAAAACTTAAGTTGATAGGTTATGAAAATACGTACAGGACAGAACAAGGAGTGCACGTACCTGATGTTAGTGGTGGTAACAGTGGACGATGAGAAGTTCTACTTGAACTTCATCATAATGGAAGTGGACAAACAACAATACATGAAGATAGAGGTAGAGATAAGTTTTGAGGTGACACCATGTGGAGGGTCAATCTTCAAGGCCCACATCGTATTCAACTCAAGAACTTTCAATGAAGAAGCAATCGAAAAGGCAAAGAATATCTTTGCAGAGCTTTTCAAGGCTTTCGAGGCCTATCTCTTGGCCGACGCTGATGCCTAA

mRNA sequence

ATGACTACTATTGTTGTGGAAGTTGACCTTGAATTTGAATCGTTCGTCCCCGCAGAGAAGTTTTTCAAGGCCATCTGCGACTTCGACAATCTTCCCAAGGTCGCCCCTCATCTTATTCAGAGCGTTGAAATCATCGAGGAAACCAAGAAGATTAACTTCACCCCAGGACAGAACAAGGAGTGCACGTACCTGATGTTAGTGGTGGTAACAGTGGACGATGAGAAGTTCTACTTGAACTTCATCATAATGGAAGTGGACAAACAACAATACATGAAGATAGAGGTAGAGATAAGTTTTGAGGTGACACCATGTGGAGGGTCAATCTTCAAGGCCCACATCGTATTCAACTCAAGAACTTTCAATGAAGAAGCAATCGAAAAGGCAAAGAATATCTTTGCAGAGCTTTTCAAGGCTTTCGAGGCCTATCTCTTGGCCGACGCTGATGCCTAA

Coding sequence (CDS)

ATGACTACTATTGTTGTGGAAGTTGACCTTGAATTTGAATCGTTCGTCCCCGCAGAGAAGTTTTTCAAGGCCATCTGCGACTTCGACAATCTTCCCAAGGTCGCCCCTCATCTTATTCAGAGCGTTGAAATCATCGAGGAAACCAAGAAGATTAACTTCACCCCAGGACAGAACAAGGAGTGCACGTACCTGATGTTAGTGGTGGTAACAGTGGACGATGAGAAGTTCTACTTGAACTTCATCATAATGGAAGTGGACAAACAACAATACATGAAGATAGAGGTAGAGATAAGTTTTGAGGTGACACCATGTGGAGGGTCAATCTTCAAGGCCCACATCGTATTCAACTCAAGAACTTTCAATGAAGAAGCAATCGAAAAGGCAAAGAATATCTTTGCAGAGCTTTTCAAGGCTTTCGAGGCCTATCTCTTGGCCGACGCTGATGCCTAA

Protein sequence

MTTIVVEVDLEFESFVPAEKFFKAICDFDNLPKVAPHLIQSVEIIEETKKINFTPGQNKECTYLMLVVVTVDDEKFYLNFIIMEVDKQQYMKIEVEISFEVTPCGGSIFKAHIVFNSRTFNEEAIEKAKNIFAELFKAFEAYLLADADA

Homology

[Close]

BLAST of Lag0022172 vs. NCBI nr
Match: XP_030974382.1 (major allergen Pru ar 1-like [Quercus lobata])

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-10
Identity = 60/158 (37.97%), Postives = 81/158 (51.27%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +P  K FKA + D DNL PKVAPH I+S EIIE        KKI F  G
Sbjct: 3   VFTYETETTSAIPPAKLFKAFVLDADNLIPKVAPHAIKSAEIIEGNGGPGTIKKITF--G 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVD--KQQYMKIEVEISFEVTPCGGSIFKAHIV 124
           +  +  Y+   +  VD   F   + ++E D   +   KI  EI    +P GGS+ K+   
Sbjct: 63  EGSQFKYVKHRIDEVDHANFTYGYSVIEGDALSEVLEKISYEIKIVASPDGGSVLKSTSK 122

Query: 125 FNSR---TFNEEAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++      EE ++  K   A LFKA EAYLLA  DA
Sbjct: 123 YHTKGEHEIKEEQVKAGKEKAAGLFKAIEAYLLAHPDA 158

[Close]

BLAST of Lag0022172 vs. NCBI nr
Match: KAF3951232.1 (hypothetical protein CMV_023096 [Castanea mollissima])

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-10
Identity = 60/158 (37.97%), Postives = 81/158 (51.27%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +P  K FKA + D DNL PKVAPH I+S EIIE        KKI F  G
Sbjct: 3   VFTYETETTSAIPPAKLFKAFVLDADNLIPKVAPHAIKSAEIIEGNGGPGTIKKITF--G 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVD--KQQYMKIEVEISFEVTPCGGSIFKAHIV 124
           +  +  Y+   +  VD   F   + ++E D   +   KI  EI    +P GGS+ K+   
Sbjct: 63  EGSQFKYVKHRIDEVDHANFTYGYSVIEGDALSEVLEKISYEIKIVASPDGGSVLKSTSK 122

Query: 125 FNSR---TFNEEAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++      EE ++  K   A LFKA EAYLLA  DA
Sbjct: 123 YHTKGEPEIKEEQVKAGKEKAAGLFKAIEAYLLAHPDA 158

[Close]

BLAST of Lag0022172 vs. NCBI nr
Match: PON61986.1 (Bet v I type allergen [Parasponia andersonii] >PON88199.1 Bet v I type allergen [Trema orientale])

HSP 1 Score: 77.0 bits (188), Expect = 1.5e-10
Identity = 60/158 (37.97%), Postives = 83/158 (52.53%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +P  + FKA I D DNL PKV P  I+S EI+E        KKINF  G
Sbjct: 3   VFTYETETISSIPPSRLFKAFILDADNLIPKVVPQAIKSTEILEGNGGPGTIKKINF--G 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVDK--QQYMKIEVEISFEVTPCGGSIFKAHIV 124
           +  +  Y+   V  +D EKF  N+ ++E D       KI  E     +P GGSI K+   
Sbjct: 63  EGSQYKYVKHQVDGLDAEKFTYNYSVIEGDALGDVVEKISYETKLVASPDGGSIIKSSSK 122

Query: 125 FNSR---TFNEEAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++      EE +++ K   + LFKA EAYLLA+ DA
Sbjct: 123 YHTKGDHEIKEENVKEGKEKASHLFKAIEAYLLANPDA 158

[Close]

BLAST of Lag0022172 vs. NCBI nr
Match: KAF3951230.1 (hypothetical protein CMV_023094 [Castanea mollissima])

HSP 1 Score: 76.3 bits (186), Expect = 2.6e-10
Identity = 60/158 (37.97%), Postives = 82/158 (51.90%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +P+ K FKA + D DNL PKVAPH I+S EIIE        KKI F  G
Sbjct: 3   VFTYETETTSAIPSAKLFKAFVLDADNLIPKVAPHAIKSAEIIEGNGGPGTIKKITF--G 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVD--KQQYMKIEVEISFEVTPCGGSIFKAHIV 124
           +  +  Y+   +  VD   F   + ++E D   +   KI  EI    +P GGSI K+   
Sbjct: 63  EGSQFKYVKHRIDEVDHANFTYCYSVIEGDALSEVLEKISYEIKIVASPDGGSILKSTSK 122

Query: 125 FNSR---TFNEEAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++      EE ++  K   A LFKA EAYL+A  DA
Sbjct: 123 YHTKGEHEIKEEQVKAGKEKAAGLFKAIEAYLVAHPDA 158

[Close]

BLAST of Lag0022172 vs. NCBI nr
Match: KAF7144680.1 (hypothetical protein RHSIM_Rhsim04G0241800 [Rhododendron simsii])

HSP 1 Score: 75.5 bits (184), Expect = 4.4e-10
Identity = 58/158 (36.71%), Postives = 81/158 (51.27%), Query Frame = 0


Query: 4   IVVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTP 63
           + +  D+E  S +PA + FKA + D DNL PKV P  I+SVEI+E        K   F  
Sbjct: 1   MAITYDIEIPSSIPAPRMFKAFVLDADNLIPKVLPQAIKSVEILEGDGGVGTVKLTTFGE 60

Query: 64  GQNKECTYLMLVVVTVDDEKFYLNFIIMEVDKQQ--YMKIEVEISFEVTPCGGSIFKAHI 123
           G   +C  +   V  VD E F  ++ I+E D     +  I   I    TP GGSI K   
Sbjct: 61  GYQHKC--VKHRVDAVDKENFTYSYSIIEGDALMGVFESISYHIKIVATPDGGSILKNRS 120

Query: 124 VFNSR---TFNEEAIEKAKNIFAELFKAFEAYLLADAD 149
           ++N++     +EE I+  K   + +FKA EAYLLA+ D
Sbjct: 121 IYNTKGDAQVSEEEIKAGKEKASGIFKAVEAYLLANPD 156

[Close]

BLAST of Lag0022172 vs. ExPASy Swiss-Prot
Match: O50001 (Major allergen Pru ar 1 OS=Prunus armeniaca OX=36596 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.1e-11
Identity = 55/158 (34.81%), Postives = 79/158 (50.00%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + EF S +P EK FKA I D DNL PKVAP  ++  EI+E        KK+ F  G
Sbjct: 3   VFTYETEFTSVIPPEKLFKAFILDADNLIPKVAPTAVKGTEILEGDGGVGTIKKVTF--G 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVD--KQQYMKIEVEISFEVTPCGGSIFKAHIV 124
           +  +  Y+   V  +D +    ++ ++E D        I  +I    +P GGSI K    
Sbjct: 63  EGSQYAYVKHRVDGIDKDNLSYSYTLIEGDALSDVIENIAYDIKLVASPDGGSIVKTTSH 122

Query: 125 FNSR---TFNEEAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++      EE ++  K   A LFK  EAYLLA+ DA
Sbjct: 123 YHTKGDVEIKEEQVKAGKEKAAGLFKLVEAYLLANPDA 158

[Close]

BLAST of Lag0022172 vs. ExPASy Swiss-Prot
Match: P43177 (Major pollen allergen Bet v 1-D/H OS=Betula pendula OX=3505 GN=BETV1D PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 7.8e-10
Identity = 58/158 (36.71%), Postives = 79/158 (50.00%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   ++E  S +PA + FKA I D DNL PKVAP  I SVE IE        KKINF  G
Sbjct: 3   VFNYEIETTSVIPAARLFKAFILDGDNLVPKVAPQAISSVENIEGNGGPGTIKKINFPEG 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVDK--QQYMKIEVEISFEVTPCGGSIFKAHIV 124
                 Y+   V  VD   F  N+ ++E         KI  EI    TP GG + K    
Sbjct: 63  --FPFKYVKDRVDEVDHTNFKYNYSVIEGGPVGDTLEKISNEIKIVATPDGGCVLKISNK 122

Query: 125 FNSRTFNE---EAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++  +E   E ++ +K +   L +A E+YLLA +DA
Sbjct: 123 YHTKGNHEVKAEQVKASKEMGETLLRAVESYLLAHSDA 158

[Close]

BLAST of Lag0022172 vs. ExPASy Swiss-Prot
Match: P43180 (Major pollen allergen Bet v 1-G OS=Betula pendula OX=3505 GN=BETV1G PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 6.6e-09
Identity = 57/158 (36.08%), Postives = 78/158 (49.37%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +PA + FKA I + DNL PKVAP  I SVE IE        KKINF  G
Sbjct: 3   VFNYESETTSVIPAARLFKAFILEGDNLIPKVAPQAISSVENIEGNGGPGTIKKINFPEG 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVDK--QQYMKIEVEISFEVTPCGGSIFKAHIV 124
                 Y+   V  VD   F  N+ ++E         KI  EI    TP GG + K    
Sbjct: 63  --FPFKYVKDRVDEVDHTNFKYNYSVIEGGPVGDTLEKISNEIKIVATPDGGCVLKISNK 122

Query: 125 FNSRTFNE---EAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++  +E   E ++ +K +   L +A E+YLLA +DA
Sbjct: 123 YHTKGNHEVKAEQVKASKEMGETLLRAVESYLLAHSDA 158

[Close]

BLAST of Lag0022172 vs. ExPASy Swiss-Prot
Match: P43185 (Major pollen allergen Bet v 1-L OS=Betula pendula OX=3505 GN=BETV1L PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 8.6e-09
Identity = 57/158 (36.08%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +PA + FKA I D D L PKVAP  I SVE IE        KKINF  G
Sbjct: 3   VFNYETEATSVIPAARMFKAFILDGDKLVPKVAPQAISSVENIEGNGGPGTIKKINFPEG 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVDK--QQYMKIEVEISFEVTPCGGSIFKAHIV 124
                 Y+   V  VD   F  N+ ++E         KI  EI    TP GG + K    
Sbjct: 63  --FPFKYVKDRVDEVDHTNFKYNYSVIEGGPVGDTLEKISNEIKIVATPDGGCVLKISNK 122

Query: 125 FNSRTFNE---EAIEKAKNIFAELFKAFEAYLLADADA 150
           ++++  +E   E ++ +K +   L +A E+YLLA +DA
Sbjct: 123 YHTKGNHEVKAEQVKASKEMGETLLRAVESYLLAHSDA 158

[Close]

BLAST of Lag0022172 vs. ExPASy Swiss-Prot
Match: P43186 (Major pollen allergen Bet v 1-M/N OS=Betula pendula OX=3505 GN=BETV1M PE=1 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 7.3e-08
Identity = 59/158 (37.34%), Postives = 76/158 (48.10%), Query Frame = 0


Query: 5   VVEVDLEFESFVPAEKFFKA-ICDFDNL-PKVAPHLIQSVEIIE------ETKKINFTPG 64
           V   + E  S +PA + FKA I D DNL PKVAP  I SVE IE        KKI F  G
Sbjct: 3   VFNYESETTSVIPAARLFKAFILDGDNLIPKVAPQAISSVENIEGNGGPGTIKKITFPEG 62

Query: 65  QNKECTYLMLVVVTVDDEKFYLNFIIMEVDK--QQYMKIEVEISFEVTPCGGSIFKAHIV 124
                 Y+   V  VD   F  ++ ++E         KI  EI    TP GGSI K    
Sbjct: 63  --SPFKYVKERVDEVDHANFKYSYSMIEGGALGDTLEKICNEIKIVATPDGGSILKISNK 122

Query: 125 FNSRTFNEEAIEKAKNIFAE---LFKAFEAYLLADADA 150
           ++++  +E   E  K I  +   L +A E+YLLA +DA
Sbjct: 123 YHTKGDHEMKAEHMKAIKEKGEALLRAVESYLLAHSDA 158

The following BLAST results are available for this feature:

BLAST of Lag0022172 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_030974382.1	1.2e-10	37.97	major allergen Pru ar 1-like [Quercus lobata]	[more]
KAF3951232.1	1.2e-10	37.97	hypothetical protein CMV_023096 [Castanea mollissima]	[more]
PON61986.1	1.5e-10	37.97	Bet v I type allergen [Parasponia andersonii] >PON88199.1 Bet v I type allergen ...	[more]
KAF3951230.1	2.6e-10	37.97	hypothetical protein CMV_023094 [Castanea mollissima]	[more]
KAF7144680.1	4.4e-10	36.71	hypothetical protein RHSIM_Rhsim04G0241800 [Rhododendron simsii]	[more]

BLAST of Lag0022172 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O50001	4.1e-11	34.81	Major allergen Pru ar 1 OS=Prunus armeniaca OX=36596 PE=1 SV=1	[more]
P43177	7.8e-10	36.71	Major pollen allergen Bet v 1-D/H OS=Betula pendula OX=3505 GN=BETV1D PE=1 SV=2	[more]
P43180	6.6e-09	36.08	Major pollen allergen Bet v 1-G OS=Betula pendula OX=3505 GN=BETV1G PE=1 SV=2	[more]
P43185	8.6e-09	36.08	Major pollen allergen Bet v 1-L OS=Betula pendula OX=3505 GN=BETV1L PE=1 SV=2	[more]
P43186	7.3e-08	37.34	Major pollen allergen Bet v 1-M/N OS=Betula pendula OX=3505 GN=BETV1M PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024949	Bet v I type allergen	PRINTS	PR00634	BETALLERGEN	coord: 5..25 score: 35.66 coord: 135..145 score: 69.92 coord: 104..120 score: 28.79 coord: 62..81 score: 22.02
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 2..149 e-value: 4.5E-27 score: 97.0
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 7..145 e-value: 6.0E-9 score: 36.0
None	No IPR available	PANTHER	PTHR31213:SF55	STRESS-INDUCED PROTEIN SAM22	coord: 6..148
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 6..148
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 8..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0022172.1	Lag0022172.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0006952	defense response
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity