Lag0022162 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0022162
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPhotosynthetic NDH subunit of subcomplex B 2
Locationchr7: 19884430 .. 19887853 (-)
RNA-Seq ExpressionLag0022162
SyntenyLag0022162
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTGGTTTGCTTTCCCCTCCCAAAGCCAAACATCGTAAGGGCTTCTTCTTCTTCTTCTCCTTCTGCAACTTCCCCTTCCACCGCCGATTCCCTCAACGACAAATTCGCTCGCAAGGGCATCCACTTCCACCACGCCAATGGCATTCCCACCGTCGACCTCACCGTCCGGAACGGCAGCTCCTTACGCCTCCACATTTCCGATGCCCATCTCACTTCCTACAAACCCAAAGTCTATTGGAAGGACGACGGCTTCGAGGAAGTTCTCTTCACTACTCCCACCTCCAATGGCGGAAAGGGAGGGATTGCCCTCGTCATCAACGATGCCTCCGATTCTGCCTCCAAAGCCTCTCTTCTCCCCTCTTTTGATTGGACTGTCAAAGATGTCGATTCGGACGCCATTGATGCTCTGCAGGTTTATATCATATTTCTATTACATCTTTGACGAAATCTGGACGGAATGAATTCATATGTCGTGTTCTTCTCCTAATTTTGTTGTGGGACTGCAGGAGCAGAAGCTCATCAATGATACAAGTTACTGTTCTGTGTTGTCTGCCTGGGATCTGAGCCCCTTTAACAAATTCTCCCTTCAAAATTGAATTTGTATTGAATGGTGTTATCAGCAGCTACATTGTGTCAACTATATTAAATAATGTTTCTCTTATAACACATTTCATGTTGGAGCTATATTACTTTGTAATTGTTTACCTCACATCTCTATATTGGTAATGATATTATCTCCCACATATTCATGTTGGAGCTATATTACTTTGTAATTGTTTACCTCACATCTCTATTTTGGTAATGATATTATCTCCCACATATTCATTTTTTACTCTAAAGGCTTTACTCAAATTAAGATTGTGTTCTTGCTTGTTAACTTTTAGTCTTCTTCACTCTAGTACTAAGTAATGAAGAAGGGTTGAAAACTTCTCATCTTTTGTTTAGACAGAATGCCAAGTTATGTAAGTTGAATGCGTTTGTATGCGGAGGTTTCATCTTGTTTTCCTTGGTGTGGTTTTGTTGCAGGTGGAGTTAAGCTGCAGTAACAAGCAAGTGGAGATTACTTACATCGTCACTCTCTATTCAGAAAGTATGGCGACCGCAGTGATCGTGAAGAACACGGGACCAAAGCCATTGACGCTAAAGAGTGCGATCCTGAGTCATTTCAAGTTCAAACGACGAGATGGGGCAGCAATCCAGGGGCTTCAAGGATGCTCCTACTGCTCTCATCCACCTCTATCTTCTCCTTTTGAAATACTGTCTCCAACAGAAGCAATGAAGGCTGAGGACCCCGATTGGTTCTCGTTCAGCTCTGAGCCTCAGGAAAAGGCAGGCTCATGGAGCCTCCAAGATGTGCCCTTCACCATTTTGAAGAACAAGTTCAGTAGAGTTTATGCCGCCCCACCAGCAGAGAGATTGAAGCCAGTATATCACACCACACCTTCAAAGTATGAGACTCTTGATCAGGTAAAAGAAAAGGCTATTGCATTGTATCACAAACATTACACCTTCAAGTTCCGTATGGAGCTCTACTTGGAAGTGCTTTAGATAGCGCTAAAAACACTTTTCAAGAAATAACATAAAAGTGTTTTGTGATAAAGCACCTCTGAAGTGTTTTTTTAGCCCGGAGAACACATTGAAAACACTATTTAGGAGTGTAGAACCAAATAAGAAACGTAGAAGTAATTTGTTATTGAGCACATTTTTAGCGAAGTGCTTATGTTAAAATTACTTTGACTAAAAGTACTTAATTTAAAAGCACTTGTAAACACGCTAATTTTGTTGAGTCTGCATATTTCAGGGGCGAGAGCTATTCTTCAGGGTGATAAGGATGGGGTTCGAAGACATATATATATCAAGCCCTGGTTCATTATCTGACAAGTTTGGGAAAGACTACTTCATATGCACTGGCCCTGCTTCCATGTTGGTTCCTGTCACAGTCAAACCTGGTGAACAATGGAGGGGTGCCCAAGTTATTGAGCATGATAACTTGTAATGTAATTATTTCCTCTTCAATCTTCATATTTTTACCCAAAATTCATTATGAAATGAACACGTGATCAAGTACTAGTAGCAGATAAAAATATTTACATATGCTCCTCCATTTTCGAGGACTTGATGAACGAGCTTCTTTCTTTATACAATATATCCACTGGTTTCCCCAATAATATAATACTAATCAAGATCAACAAGTTGGCTCTGTTATTGTTGTCATGTTCTATTATAATATTGATCCATGAGCAGGACAGCTACTTTTTTATTCACTAGAATTTATCAATCTCAACCTCCTGTGAGTACAATGGCTTCAGAATCAGGCCCTGTGGTGGCTGCTCTGCCGCTGCAGGATCGAGTGGCCATTGTGACCGGTGCCTCTCGGGGCATCGGTCGAGCCATTGCCCTTCACCTCGCCGCTCTTGGTGCCCGGCTTGTCATCAACTACGTCTCCGATTCAGCCAAAGCTGATCAAGTTGCTGCTGAGATCAACTCATCATCGGCCGCTGGGTGCCAACGAGCCATTGCCTGGCGGGCAGATGTGTCTGATCCAGAGCAAGTGAAGTCCTTGTTTGACGCAGCCGAGCAGGCATTTGGCTCCCAAGTCCATATCCTGGTTAACTCTGCTGGAATTTCGGATCCTACATACCCTTGTATCGCAGATACTTCACTGGAGATTTTCGACCAAATTTTCAGGTCCCTCTCCCTCTTCTTTCATGAAATTTCTTTGGGAAAATTTTAATTTTCAAATGGTTGATGAGTTAATAATATTGATCTTAAAAACTTTTGATTCCATCCAATAGAGTTTTTAGTTAAATCGTCATTAAACCAAAAAGCCTAAGAAGATGAGTTATAAACTTGGCAATCTACACAAGTCTCATTTAGCAGCTATCAATATGAACTAGGCAAGAAAATGACTATATTGGAATTTGATTGTAGAACTTCCTTTTCCAATATGGAGTTAAATTAAACTGATGGGTTACGGTAAATTTAAATGGACGAGTGTGTTTTTCAGTGTGAACACGAGGGGTAGTTTCCTGTGCTGCAGAGAAGCAGCAACCCGAGTGAAACACGGCGGTGGGGGGCGGATCATACTGATATCATCAACAGCAGTGGCAGCACCAACAGCAGGATTAGGGGCATACACAGCATCAAAGGCGGCAGTGGAGGCAATGACGAAGGTGGCAGCGAAGGAGCTGAGAGGGAGCGGAATAACAGTGAACTGCATAGCGCCCGGGGCAACGGCGACGGAGATGTTTTACAGAGGGATGGATGAGGAAGCAGTGAAAAAAGTGATTGAGAAGTGTCCGCTGGGGAGGCTTGGGGATCCCAAAGATGTGGCTTCTTTTGTTGGGTTTTTGGCGTCCGATGATGGCGAGTGGATCAATGGCCAAGTCGTGCTCGTCAATGGCGGCATTGTGTAG

mRNA sequence

ATGGCTTCTCTGGTTTGCTTTCCCCTCCCAAAGCCAAACATCGTAAGGGCTTCTTCTTCTTCTTCTCCTTCTGCAACTTCCCCTTCCACCGCCGATTCCCTCAACGACAAATTCGCTCGCAAGGGCATCCACTTCCACCACGCCAATGGCATTCCCACCGTCGACCTCACCGTCCGGAACGGCAGCTCCTTACGCCTCCACATTTCCGATGCCCATCTCACTTCCTACAAACCCAAAGTCTATTGGAAGGACGACGGCTTCGAGGAAGTTCTCTTCACTACTCCCACCTCCAATGGCGGAAAGGGAGGGATTGCCCTCGTCATCAACGATGCCTCCGATTCTGCCTCCAAAGCCTCTCTTCTCCCCTCTTTTGATTGGACTGTCAAAGATGTCGATTCGGACGCCATTGATGCTCTGCAGGTGGAGTTAAGCTGCAGTAACAAGCAAGTGGAGATTACTTACATCGTCACTCTCTATTCAGAAAGTATGGCGACCGCAGTGATCGTGAAGAACACGGGACCAAAGCCATTGACGCTAAAGAGTGCGATCCTGAGTCATTTCAAGTTCAAACGACGAGATGGGGCAGCAATCCAGGGGCTTCAAGGATGCTCCTACTGCTCTCATCCACCTCTATCTTCTCCTTTTGAAATACTGTCTCCAACAGAAGCAATGAAGGCTGAGGACCCCGATTGGTTCTCGTTCAGCTCTGAGCCTCAGGAAAAGGCAGGCTCATGGAGCCTCCAAGATGTGCCCTTCACCATTTTGAAGAACAAGTTCAGTAGAGTTTATGCCGCCCCACCAGCAGAGAGATTGAAGCCAGTATATCACACCACACCTTCAAAGTATGAGACTCTTGATCAGGGGCGAGAGCTATTCTTCAGGGTGATAAGGATGGGGTTCGAAGACATATATATATCAAGCCCTGGTTCATTATCTGACAAGTTTGGGAAAGACTACTTCATATGCACTGGCCCTGCTTCCATGTTGGTTCCTGTCACAGTCAAACCTGGTGAACAATGGAGGGGTGCCCAAGTTATTGAGCATGATAACTTGACAGCTACTTTTTTATTCACTAGAATTTATCAATCTCAACCTCCTGTGAGTACAATGGCTTCAGAATCAGGCCCTGTGGTGGCTGCTCTGCCGCTGCAGGATCGAGTGGCCATTGTGACCGGTGCCTCTCGGGGCATCGGTCGAGCCATTGCCCTTCACCTCGCCGCTCTTGGTGCCCGGCTTGTCATCAACTACGTCTCCGATTCAGCCAAAGCTGATCAAGTTGCTGCTGAGATCAACTCATCATCGGCCGCTGGGTGCCAACGAGCCATTGCCTGGCGGGCAGATGTGTCTGATCCAGAGCAAGTGAAGTCCTTGTTTGACGCAGCCGAGCAGGCATTTGGCTCCCAAGTCCATATCCTGGTTAACTCTGCTGGAATTTCGGATCCTACATACCCTTGTATCGCAGATACTTCACTGGAGATTTTCGACCAAATTTTCAGAGAAGCAGCAACCCGAGTGAAACACGGCGGTGGGGGGCGGATCATACTGATATCATCAACAGCAGTGGCAGCACCAACAGCAGGATTAGGGGCATACACAGCATCAAAGGCGGCAGTGGAGGCAATGACGAAGGTGGCAGCGAAGGAGCTGAGAGGGAGCGGAATAACAGTGAACTGCATAGCGCCCGGGGCAACGGCGACGGAGATGTTTTACAGAGGGATGGATGAGGAAGCAGTGAAAAAAGTGATTGAGAAGTGTCCGCTGGGGAGGCTTGGGGATCCCAAAGATGTGGCTTCTTTTGTTGGGTTTTTGGCGTCCGATGATGGCGAGTGGATCAATGGCCAAGTCGTGCTCGTCAATGGCGGCATTGTGTAG

Coding sequence (CDS)

ATGGCTTCTCTGGTTTGCTTTCCCCTCCCAAAGCCAAACATCGTAAGGGCTTCTTCTTCTTCTTCTCCTTCTGCAACTTCCCCTTCCACCGCCGATTCCCTCAACGACAAATTCGCTCGCAAGGGCATCCACTTCCACCACGCCAATGGCATTCCCACCGTCGACCTCACCGTCCGGAACGGCAGCTCCTTACGCCTCCACATTTCCGATGCCCATCTCACTTCCTACAAACCCAAAGTCTATTGGAAGGACGACGGCTTCGAGGAAGTTCTCTTCACTACTCCCACCTCCAATGGCGGAAAGGGAGGGATTGCCCTCGTCATCAACGATGCCTCCGATTCTGCCTCCAAAGCCTCTCTTCTCCCCTCTTTTGATTGGACTGTCAAAGATGTCGATTCGGACGCCATTGATGCTCTGCAGGTGGAGTTAAGCTGCAGTAACAAGCAAGTGGAGATTACTTACATCGTCACTCTCTATTCAGAAAGTATGGCGACCGCAGTGATCGTGAAGAACACGGGACCAAAGCCATTGACGCTAAAGAGTGCGATCCTGAGTCATTTCAAGTTCAAACGACGAGATGGGGCAGCAATCCAGGGGCTTCAAGGATGCTCCTACTGCTCTCATCCACCTCTATCTTCTCCTTTTGAAATACTGTCTCCAACAGAAGCAATGAAGGCTGAGGACCCCGATTGGTTCTCGTTCAGCTCTGAGCCTCAGGAAAAGGCAGGCTCATGGAGCCTCCAAGATGTGCCCTTCACCATTTTGAAGAACAAGTTCAGTAGAGTTTATGCCGCCCCACCAGCAGAGAGATTGAAGCCAGTATATCACACCACACCTTCAAAGTATGAGACTCTTGATCAGGGGCGAGAGCTATTCTTCAGGGTGATAAGGATGGGGTTCGAAGACATATATATATCAAGCCCTGGTTCATTATCTGACAAGTTTGGGAAAGACTACTTCATATGCACTGGCCCTGCTTCCATGTTGGTTCCTGTCACAGTCAAACCTGGTGAACAATGGAGGGGTGCCCAAGTTATTGAGCATGATAACTTGACAGCTACTTTTTTATTCACTAGAATTTATCAATCTCAACCTCCTGTGAGTACAATGGCTTCAGAATCAGGCCCTGTGGTGGCTGCTCTGCCGCTGCAGGATCGAGTGGCCATTGTGACCGGTGCCTCTCGGGGCATCGGTCGAGCCATTGCCCTTCACCTCGCCGCTCTTGGTGCCCGGCTTGTCATCAACTACGTCTCCGATTCAGCCAAAGCTGATCAAGTTGCTGCTGAGATCAACTCATCATCGGCCGCTGGGTGCCAACGAGCCATTGCCTGGCGGGCAGATGTGTCTGATCCAGAGCAAGTGAAGTCCTTGTTTGACGCAGCCGAGCAGGCATTTGGCTCCCAAGTCCATATCCTGGTTAACTCTGCTGGAATTTCGGATCCTACATACCCTTGTATCGCAGATACTTCACTGGAGATTTTCGACCAAATTTTCAGAGAAGCAGCAACCCGAGTGAAACACGGCGGTGGGGGGCGGATCATACTGATATCATCAACAGCAGTGGCAGCACCAACAGCAGGATTAGGGGCATACACAGCATCAAAGGCGGCAGTGGAGGCAATGACGAAGGTGGCAGCGAAGGAGCTGAGAGGGAGCGGAATAACAGTGAACTGCATAGCGCCCGGGGCAACGGCGACGGAGATGTTTTACAGAGGGATGGATGAGGAAGCAGTGAAAAAAGTGATTGAGAAGTGTCCGCTGGGGAGGCTTGGGGATCCCAAAGATGTGGCTTCTTTTGTTGGGTTTTTGGCGTCCGATGATGGCGAGTGGATCAATGGCCAAGTCGTGCTCGTCAATGGCGGCATTGTGTAG

Protein sequence

MASLVCFPLPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVRNGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKASLLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTLKSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMGFEDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNLTATFLFTRIYQSQPPVSTMASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQVAAEINSSSAAGCQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPCIADTSLEIFDQIFREAATRVKHGGGGRIILISSTAVAAPTAGLGAYTASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDDGEWINGQVVLVNGGIV
Homology
BLAST of Lag0022162 vs. NCBI nr
Match: KAA0051280.1 (photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK29981.1 photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1050.8 bits (2716), Expect = 4.6e-303
Identity = 542/635 (85.35%), Postives = 581/635 (91.50%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVRN 60
           MASLVC PLPKPN++RASSSSS S + PSTADSLNDKFARKG HFH +NG+PT+DLTVRN
Sbjct: 1   MASLVCLPLPKPNLIRASSSSS-STSFPSTADSLNDKFARKGFHFHDSNGVPTLDLTVRN 60

Query: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKASL 120
           GSSLRLHISDAHLTSYKPKVYWKDDGFEE+LFTTPT+   KGGIALVI+DASDS+SK+SL
Sbjct: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEILFTTPTA---KGGIALVIDDASDSSSKSSL 120

Query: 121 LPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTLK 180
           LPS DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSES+ATAVIVKNTG KPLTLK
Sbjct: 121 LPSSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESLATAVIVKNTGLKPLTLK 180

Query: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQE 240
           SAILSHFKFK+RDGAAIQGLQGCSYCSHPPLSSPFEILSP+EAMK E+PDWFSF SEPQ 
Sbjct: 181 SAILSHFKFKQRDGAAIQGLQGCSYCSHPPLSSPFEILSPSEAMKVEEPDWFSFGSEPQG 240

Query: 241 KAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMGF 300
           KAGSWSLQDVPFT+LKNKFSRVYAAPPAERLKP+YHTTPSKYETLDQGRELFFRVIRMGF
Sbjct: 241 KAGSWSLQDVPFTMLKNKFSRVYAAPPAERLKPIYHTTPSKYETLDQGRELFFRVIRMGF 300

Query: 301 EDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNLTATFLFTRI 360
           EDIY+SSPGSLS+KFGKD+FICTGPASMLVPVTVKPGEQW+GAQ     +  ATFL TRI
Sbjct: 301 EDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWKGAQ-YRSISSAATFLITRI 360

Query: 361 YQSQPPVSTMASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDS 420
           YQSQP VSTMASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV+ S
Sbjct: 361 YQSQPLVSTMASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASS 420

Query: 421 AKADQVAAEINSSSAAG-CQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS 480
           A+ADQV A INS+SAAG  QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS
Sbjct: 421 AEADQVVASINSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS 480

Query: 481 DPTYPCIADTSLEIFDQIF-----------REAATRVKHGGGGRIILISSTAVAAPTAGL 540
           DPTYP IADT LEIFD +F           +EAA RVK GGGGRIILISSTAV A TAGL
Sbjct: 481 DPTYPYIADTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVVATTAGL 540

Query: 541 GAYTASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLG 600
           GAYTASKAAVEAM KVAAKEL G+GI+VNCIAPGATATEMFY+G++EE VKKVIEKCP+G
Sbjct: 541 GAYTASKAAVEAMAKVAAKELSGTGISVNCIAPGATATEMFYKGINEEGVKKVIEKCPMG 600

Query: 601 RLGDPKDVASFVGFLASDDGEWINGQVVLVNGGIV 624
           R+G PKDVASFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 601 RIGVPKDVASFVGFLASDDGEWINGQVILVNGGIV 630

BLAST of Lag0022162 vs. NCBI nr
Match: KGN59874.2 (hypothetical protein Csa_001420 [Cucumis sativus])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.5e-301
Identity = 541/635 (85.20%), Postives = 576/635 (90.71%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVRN 60
           MASLVC PLPKPN++RASSSSS S + PST DSLNDKF RKG HFH +NG+PT+DLTVRN
Sbjct: 1   MASLVCLPLPKPNLIRASSSSSSSTSFPSTVDSLNDKFGRKGFHFHDSNGVPTLDLTVRN 60

Query: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKASL 120
           GSSLRL ISDAHLTSYKPKVYWKDDGFEEVLFTTPT+   KGGIALVI+DASDS+SK+SL
Sbjct: 61  GSSLRLRISDAHLTSYKPKVYWKDDGFEEVLFTTPTA---KGGIALVIDDASDSSSKSSL 120

Query: 121 LPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTLK 180
           LPS DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSES+ATAVIVKNTG KPLTLK
Sbjct: 121 LPSSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESLATAVIVKNTGLKPLTLK 180

Query: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQE 240
           SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSP+EAMK E+ DWFSF SEPQ 
Sbjct: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPSEAMKVEESDWFSFGSEPQG 240

Query: 241 KAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMGF 300
           KAGSWSLQDVPFT+LKNKFSRVYAAPPAERLKP+YHTTPSKYETLDQGRELFFRVIRMGF
Sbjct: 241 KAGSWSLQDVPFTMLKNKFSRVYAAPPAERLKPIYHTTPSKYETLDQGRELFFRVIRMGF 300

Query: 301 EDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNLTATFLFTRI 360
           EDIY+SSPGSLS+KFGKD+FICTGPASMLVPVTVKPGEQWRGAQ     +  A FL T I
Sbjct: 301 EDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWRGAQ-YRSISSAAAFLSTII 360

Query: 361 YQSQPPVSTMASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDS 420
           YQSQP VSTMASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYVS S
Sbjct: 361 YQSQPLVSTMASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSS 420

Query: 421 AKADQVAAEINSSSAAG-CQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS 480
           A+ADQV A+INSSSAAG  QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS
Sbjct: 421 AEADQVVADINSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS 480

Query: 481 DPTYPCIADTSLEIFDQIF-----------REAATRVKHGGGGRIILISSTAVAAPTAGL 540
           DPTYP IA+T LEIFD +F           +EAA RVK GGGGRIILISSTAVAA TAGL
Sbjct: 481 DPTYPYIANTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVAATTAGL 540

Query: 541 GAYTASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLG 600
           GAYTASKAAVEAM KV AKEL G+GI+VNCIAPGATATEMFY+G+DEE VKKVI+KCP+G
Sbjct: 541 GAYTASKAAVEAMAKVTAKELSGTGISVNCIAPGATATEMFYKGIDEEGVKKVIDKCPMG 600

Query: 601 RLGDPKDVASFVGFLASDDGEWINGQVVLVNGGIV 624
           RLG PKDVASFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 601 RLGVPKDVASFVGFLASDDGEWINGQVILVNGGIV 631

BLAST of Lag0022162 vs. NCBI nr
Match: XP_022972247.1 (photosynthetic NDH subunit of subcomplex B 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 649.8 bits (1675), Expect = 2.4e-182
Identity = 324/358 (90.50%), Postives = 336/358 (93.85%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSS-------SPSATSPSTADSLNDKFARKGIHFHHANGIPT 60
           MASLVC PLPK +IVRASSSS       SPSATSP  ADSLNDKF RKGIHFH +NGIPT
Sbjct: 1   MASLVCLPLPKLSIVRASSSSSSFSSSLSPSATSPLPADSLNDKFGRKGIHFHQSNGIPT 60

Query: 61  VDLTVRNGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASD 120
           VDLTVRNGSSLRLHISDAH+TSYKPKVYWKDDGFEEVLFTTP +NGGKGGI LVINDAS+
Sbjct: 61  VDLTVRNGSSLRLHISDAHITSYKPKVYWKDDGFEEVLFTTPAANGGKGGIGLVINDASN 120

Query: 121 SASKASLLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTG 180
           SASK+SLLP+ DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSESMATAVIVKNTG
Sbjct: 121 SASKSSLLPTSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESMATAVIVKNTG 180

Query: 181 PKPLTLKSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFS 240
           PK L+L SAILSHFKFKRRDGAAIQGL+GCSYCS PPLSSPFEILSPTEAMKAEDPDWFS
Sbjct: 181 PKLLSLNSAILSHFKFKRRDGAAIQGLEGCSYCSLPPLSSPFEILSPTEAMKAEDPDWFS 240

Query: 241 FSSEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFF 300
           F SEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAER KPVYHTTPSKYETLDQGRELFF
Sbjct: 241 FGSEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERSKPVYHTTPSKYETLDQGRELFF 300

Query: 301 RVIRMGFEDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           RVIRMGFEDIY+ SPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL
Sbjct: 301 RVIRMGFEDIYLQSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 358

BLAST of Lag0022162 vs. NCBI nr
Match: XP_022932774.1 (photosynthetic NDH subunit of subcomplex B 2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 648.3 bits (1671), Expect = 6.8e-182
Identity = 323/355 (90.99%), Postives = 334/355 (94.08%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSS----SPSATSPSTADSLNDKFARKGIHFHHANGIPTVDL 60
           MASLVC PLPK +IVRASSSS    SPSATSP  ADSLNDKF RKGIHFH +NGIPTVDL
Sbjct: 1   MASLVCLPLPKLSIVRASSSSSSSLSPSATSPLPADSLNDKFGRKGIHFHQSNGIPTVDL 60

Query: 61  TVRNGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSAS 120
           TVRNGSSLRLHISDAH+ SYKPKVYWKDDGFEEVLFTTP +NGGKGGI LVINDAS+SAS
Sbjct: 61  TVRNGSSLRLHISDAHIPSYKPKVYWKDDGFEEVLFTTPAANGGKGGIGLVINDASNSAS 120

Query: 121 KASLLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKP 180
           K SLLP+ DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSESMATAVIVKNTGPK 
Sbjct: 121 KPSLLPTSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESMATAVIVKNTGPKL 180

Query: 181 LTLKSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSS 240
           L+L SAILSHFKFKRRDGAAIQGL+GCSYCS PPLSSPFEILSPTEAMKAEDPDWFSF S
Sbjct: 181 LSLNSAILSHFKFKRRDGAAIQGLEGCSYCSLPPLSSPFEILSPTEAMKAEDPDWFSFGS 240

Query: 241 EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVI 300
           EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAER KPVYHTTPSKYETLDQGRELFFRVI
Sbjct: 241 EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERSKPVYHTTPSKYETLDQGRELFFRVI 300

Query: 301 RMGFEDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           RMGFEDIY+ SPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL
Sbjct: 301 RMGFEDIYVQSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 355

BLAST of Lag0022162 vs. NCBI nr
Match: XP_023540761.1 (photosynthetic NDH subunit of subcomplex B 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 646.7 bits (1667), Expect = 2.0e-181
Identity = 322/355 (90.70%), Postives = 334/355 (94.08%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSS----SPSATSPSTADSLNDKFARKGIHFHHANGIPTVDL 60
           MASLVC PLPK +IVRASSSS    SPSATSP  ADSLNDKF RKGIHFH +NGIPTVDL
Sbjct: 1   MASLVCLPLPKLSIVRASSSSSSSLSPSATSPLPADSLNDKFGRKGIHFHQSNGIPTVDL 60

Query: 61  TVRNGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSAS 120
           TVRNGSSLRLHISDAH+ SYKPKVYWKDDGFEEVLFTTP +NGGKGGI LVINDAS+SAS
Sbjct: 61  TVRNGSSLRLHISDAHIPSYKPKVYWKDDGFEEVLFTTPAANGGKGGIGLVINDASNSAS 120

Query: 121 KASLLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKP 180
           K SLLP+ DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSESMATAVIVKNTGPK 
Sbjct: 121 KPSLLPTSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESMATAVIVKNTGPKL 180

Query: 181 LTLKSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSS 240
           L+L SAILSHFKFKRRDGAAIQGL+GCSYCS PPLSSPFEILSPTEAMKAEDPDWFSFSS
Sbjct: 181 LSLNSAILSHFKFKRRDGAAIQGLEGCSYCSLPPLSSPFEILSPTEAMKAEDPDWFSFSS 240

Query: 241 EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVI 300
           EPQEK GSWSLQDVPFTILKNKFSRVYAAPPAER KPVYHTTPSKYETLDQGRELFFRVI
Sbjct: 241 EPQEKVGSWSLQDVPFTILKNKFSRVYAAPPAERSKPVYHTTPSKYETLDQGRELFFRVI 300

Query: 301 RMGFEDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           RMGFEDIY+ SPGSLSDKFGKDYFICTGPASMLVPVTV+PGEQWRGAQVIEHDNL
Sbjct: 301 RMGFEDIYVQSPGSLSDKFGKDYFICTGPASMLVPVTVEPGEQWRGAQVIEHDNL 355

BLAST of Lag0022162 vs. ExPASy Swiss-Prot
Match: Q94AQ8 (Photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PNSB2 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 3.3e-118
Identity = 215/353 (60.91%), Postives = 270/353 (76.49%), Query Frame = 0

Query: 1   MASLVCFP-LPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVR 60
           MASL+ F  LPKP  VR SS S+P   + +T + L DKF RKGI F  +N IP V+L VR
Sbjct: 1   MASLISFSLLPKPKAVR-SSISAPQTQTINT-EKLEDKFGRKGIKFSESNNIPMVELKVR 60

Query: 61  NGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKAS 120
           NGSSL+L +SDAH+ SYKPKVYWKD+GFEEVL+T    +  +GG+ +VI +  +    +S
Sbjct: 61  NGSSLKLSLSDAHVLSYKPKVYWKDEGFEEVLYTV-DGDESRGGVGVVIVNGEEPKGGSS 120

Query: 121 LLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTL 180
           ++   DW+VKD DSDAIDALQ+ELSC+   ++ITYIV+LY  SMATA++VKN G KP+TL
Sbjct: 121 VISGCDWSVKDTDSDAIDALQIELSCTAGVLDITYIVSLYPVSMATALVVKNNGRKPVTL 180

Query: 181 KSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQ 240
           K  I+S+ +FK+R GA IQGL+GCSYC +PPLSSPFE+LSP+EAMKAE   W  F SE  
Sbjct: 181 KPGIMSYLRFKKRSGAGIQGLKGCSYCPNPPLSSPFELLSPSEAMKAESSGW--FGSEEG 240

Query: 241 EKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMG 300
           EK G W+++D   T+L+ K SR+Y APPAERLK VY+T PSK+ET+DQGR LFFR+IR+G
Sbjct: 241 EKPGIWAVEDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQGRGLFFRMIRIG 300

Query: 301 FEDIYISSPGSLSDKFGKD-YFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           FE++Y+ SPGS+ DK+GK  YF+CTGP SMLVPV V  GE WRGA VIEHDNL
Sbjct: 301 FEEMYVGSPGSMWDKYGKQHYFVCTGPTSMLVPVDVASGETWRGAMVIEHDNL 348

BLAST of Lag0022162 vs. ExPASy Swiss-Prot
Match: Q9SQR4 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g03980 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.4e-73
Identity = 157/273 (57.51%), Postives = 191/273 (69.96%), Query Frame = 0

Query: 367 VSTMASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQV 426
           +ST +S S P    LPL  RVAIVTG+SRGIGRAIA+HLA LGAR+VINY S +A A++V
Sbjct: 1   MSTHSSISQP---PLPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 60

Query: 427 AAEIN-----SSSAAGCQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDP 486
           A+EIN             RAI  +A+VS+P QVKS+FDAAE AF + VHILVNSAGI DP
Sbjct: 61  ASEINDFPVREEITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDP 120

Query: 487 TYPCIADTSLEIFDQIF-----------REAATRVKHGGGGRIILISSTAVAAPTAGLGA 546
            YP IADTS+E FD  F           +EAA R+K GGGGRIIL++S+   +   G GA
Sbjct: 121 KYPTIADTSVEDFDHTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGA 180

Query: 547 YTASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRL 606
           Y ASKAAVE M K+ AKEL+G+GIT NC+APG  ATEMF+ G   E V+K+  + P GR+
Sbjct: 181 YAASKAAVETMVKILAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRV 240

Query: 607 GDPKDVASFVGFLASDDGEWINGQVVLVNGGIV 624
           G+ KDV   VGFLA D GEW+NGQ++ VNGG V
Sbjct: 241 GEAKDVVPLVGFLAGDGGEWVNGQIIPVNGGYV 270

BLAST of Lag0022162 vs. ExPASy Swiss-Prot
Match: Q9SQR2 (NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR2 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.8e-69
Identity = 146/269 (54.28%), Postives = 187/269 (69.52%), Query Frame = 0

Query: 370 MASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQVAAE 429
           MA+ S      L L  RVAIVTG+SRGIGRAIA+HLA LGAR+V+NY +   +A++VA  
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 430 INSSSAAGCQ------RAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTY 489
           I ++ +   +      R I  +AD+S+P QVKSLFD AE+ F S VHILVNSA I+DP +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 490 PCIADTSLEIFDQIF-----------REAATRVKHGGGGRIILISSTAVAAPTAGLGAYT 549
             I+D S+E+FD+I            REAA R+K GGGGRIIL+S++ V       G+YT
Sbjct: 121 STISDMSVELFDRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYT 180

Query: 550 ASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRLGD 609
           ASKAAVEAM K+ AKEL+G+ ITVNC++PG  ATEMFY G+  E V+KV  +   GR+G+
Sbjct: 181 ASKAAVEAMAKILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGE 240

Query: 610 PKDVASFVGFLASDDGEWINGQVVLVNGG 622
            KD+A  VGFLASD GEWINGQV++ NGG
Sbjct: 241 TKDIAPVVGFLASDAGEWINGQVIMANGG 269

BLAST of Lag0022162 vs. ExPASy Swiss-Prot
Match: Q08632 (Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 7.9e-64
Identity = 144/256 (56.25%), Postives = 179/256 (69.92%), Query Frame = 0

Query: 381 LPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQVAAEIN--SSSAAGC 440
           LPL  RVAIVTGASRGIGR IAL++A  GA++VI+Y S+   A++VA+ IN  S S+   
Sbjct: 17  LPLGGRVAIVTGASRGIGREIALNMAEKGAKVVIHYSSNQHAAEEVASIINNKSPSSGDG 76

Query: 441 QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPCIADTSLEIFDQIF 500
            RAI  +ADV++P QV  LFD AE AFG  +HI+VN+AG++D  YP +A TS E +D+IF
Sbjct: 77  VRAIVCKADVAEPSQVAQLFDTAEHAFG-PLHIVVNNAGVTDSKYPTLAQTSDEEWDRIF 136

Query: 501 -----------REAATRVKHGGGGRIILISSTAVAAPTAGLGAYTASKAAVEAMTKVAAK 560
                      REAA RV  GGGGRII ISS+ VA P    GAYTASKAAVE MT++ A+
Sbjct: 137 QVNCKGAFLCSREAAKRVVRGGGGRIINISSSLVAMPIPRYGAYTASKAAVEMMTRILAQ 196

Query: 561 ELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDD 620
           ELRG+ IT NC+APG  AT+MF+ G  E AV+  ++  P  RLG  +DVA  V FLASD+
Sbjct: 197 ELRGTQITANCVAPGPVATDMFFAGKSEAAVEAGVKSNPFERLGKVEDVAPLVAFLASDE 256

Query: 621 GEWINGQVVLVNGGIV 624
           GEW+N QVV VNGG V
Sbjct: 257 GEWVNAQVVRVNGGQV 271

BLAST of Lag0022162 vs. ExPASy Swiss-Prot
Match: P51831 (3-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Bacillus subtilis (strain 168) OX=224308 GN=fabG PE=3 SV=3)

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-34
Identity = 97/250 (38.80%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 383 LQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQVAAEINSSSAAGCQRAI 442
           L D+ AIVTGASRGIGR+IAL LA  GA +V+NY  + AKA++V  EI S      ++AI
Sbjct: 2   LNDKTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEIKSMG----RKAI 61

Query: 443 AWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS---------DPTYPCIADTSLEI 502
           A +ADVS+PE V+++       F S + ILVN+AGI+         +  +  + + +L+ 
Sbjct: 62  AVKADVSNPEDVQNMIKETLSVF-STIDILVNNAGITRDNLIMRMKEDEWDDVININLKG 121

Query: 503 FDQIFREAATRVKHGGGGRIILISSTAVAAPTAGLGAYTASKAAVEAMTKVAAKELRGSG 562
                +    ++     GRII +SS    +   G   Y A+KA V  +TK +AKEL    
Sbjct: 122 VFNCTKAVTRQMMKQRSGRIINVSSIVGVSGNPGQANYVAAKAGVIGLTKSSAKELASRN 181

Query: 563 ITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDDGEWING 622
           ITVN IAPG  +T+M  + + ++   +++++ PL R G+P DV+S V FLAS+   ++ G
Sbjct: 182 ITVNAIAPGFISTDMTDK-LAKDVQDEMLKQIPLARFGEPSDVSSVVTFLASEGARYMTG 241

Query: 623 QVVLVNGGIV 624
           Q + ++GG+V
Sbjct: 242 QTLHIDGGMV 245

BLAST of Lag0022162 vs. ExPASy TrEMBL
Match: A0A5A7U7W3 (Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00100 PE=4 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 2.2e-303
Identity = 542/635 (85.35%), Postives = 581/635 (91.50%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVRN 60
           MASLVC PLPKPN++RASSSSS S + PSTADSLNDKFARKG HFH +NG+PT+DLTVRN
Sbjct: 1   MASLVCLPLPKPNLIRASSSSS-STSFPSTADSLNDKFARKGFHFHDSNGVPTLDLTVRN 60

Query: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKASL 120
           GSSLRLHISDAHLTSYKPKVYWKDDGFEE+LFTTPT+   KGGIALVI+DASDS+SK+SL
Sbjct: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEILFTTPTA---KGGIALVIDDASDSSSKSSL 120

Query: 121 LPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTLK 180
           LPS DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSES+ATAVIVKNTG KPLTLK
Sbjct: 121 LPSSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESLATAVIVKNTGLKPLTLK 180

Query: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQE 240
           SAILSHFKFK+RDGAAIQGLQGCSYCSHPPLSSPFEILSP+EAMK E+PDWFSF SEPQ 
Sbjct: 181 SAILSHFKFKQRDGAAIQGLQGCSYCSHPPLSSPFEILSPSEAMKVEEPDWFSFGSEPQG 240

Query: 241 KAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMGF 300
           KAGSWSLQDVPFT+LKNKFSRVYAAPPAERLKP+YHTTPSKYETLDQGRELFFRVIRMGF
Sbjct: 241 KAGSWSLQDVPFTMLKNKFSRVYAAPPAERLKPIYHTTPSKYETLDQGRELFFRVIRMGF 300

Query: 301 EDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNLTATFLFTRI 360
           EDIY+SSPGSLS+KFGKD+FICTGPASMLVPVTVKPGEQW+GAQ     +  ATFL TRI
Sbjct: 301 EDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWKGAQ-YRSISSAATFLITRI 360

Query: 361 YQSQPPVSTMASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDS 420
           YQSQP VSTMASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV+ S
Sbjct: 361 YQSQPLVSTMASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASS 420

Query: 421 AKADQVAAEINSSSAAG-CQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS 480
           A+ADQV A INS+SAAG  QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS
Sbjct: 421 AEADQVVASINSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGIS 480

Query: 481 DPTYPCIADTSLEIFDQIF-----------REAATRVKHGGGGRIILISSTAVAAPTAGL 540
           DPTYP IADT LEIFD +F           +EAA RVK GGGGRIILISSTAV A TAGL
Sbjct: 481 DPTYPYIADTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVVATTAGL 540

Query: 541 GAYTASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLG 600
           GAYTASKAAVEAM KVAAKEL G+GI+VNCIAPGATATEMFY+G++EE VKKVIEKCP+G
Sbjct: 541 GAYTASKAAVEAMAKVAAKELSGTGISVNCIAPGATATEMFYKGINEEGVKKVIEKCPMG 600

Query: 601 RLGDPKDVASFVGFLASDDGEWINGQVVLVNGGIV 624
           R+G PKDVASFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 601 RIGVPKDVASFVGFLASDDGEWINGQVILVNGGIV 630

BLAST of Lag0022162 vs. ExPASy TrEMBL
Match: A0A6J1I4B2 (photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470832 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 1.1e-182
Identity = 324/358 (90.50%), Postives = 336/358 (93.85%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSS-------SPSATSPSTADSLNDKFARKGIHFHHANGIPT 60
           MASLVC PLPK +IVRASSSS       SPSATSP  ADSLNDKF RKGIHFH +NGIPT
Sbjct: 1   MASLVCLPLPKLSIVRASSSSSSFSSSLSPSATSPLPADSLNDKFGRKGIHFHQSNGIPT 60

Query: 61  VDLTVRNGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASD 120
           VDLTVRNGSSLRLHISDAH+TSYKPKVYWKDDGFEEVLFTTP +NGGKGGI LVINDAS+
Sbjct: 61  VDLTVRNGSSLRLHISDAHITSYKPKVYWKDDGFEEVLFTTPAANGGKGGIGLVINDASN 120

Query: 121 SASKASLLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTG 180
           SASK+SLLP+ DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSESMATAVIVKNTG
Sbjct: 121 SASKSSLLPTSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESMATAVIVKNTG 180

Query: 181 PKPLTLKSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFS 240
           PK L+L SAILSHFKFKRRDGAAIQGL+GCSYCS PPLSSPFEILSPTEAMKAEDPDWFS
Sbjct: 181 PKLLSLNSAILSHFKFKRRDGAAIQGLEGCSYCSLPPLSSPFEILSPTEAMKAEDPDWFS 240

Query: 241 FSSEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFF 300
           F SEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAER KPVYHTTPSKYETLDQGRELFF
Sbjct: 241 FGSEPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERSKPVYHTTPSKYETLDQGRELFF 300

Query: 301 RVIRMGFEDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           RVIRMGFEDIY+ SPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL
Sbjct: 301 RVIRMGFEDIYLQSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 358

BLAST of Lag0022162 vs. ExPASy TrEMBL
Match: A0A6J1EXZ1 (photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439223 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 3.3e-182
Identity = 323/355 (90.99%), Postives = 334/355 (94.08%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSS----SPSATSPSTADSLNDKFARKGIHFHHANGIPTVDL 60
           MASLVC PLPK +IVRASSSS    SPSATSP  ADSLNDKF RKGIHFH +NGIPTVDL
Sbjct: 1   MASLVCLPLPKLSIVRASSSSSSSLSPSATSPLPADSLNDKFGRKGIHFHQSNGIPTVDL 60

Query: 61  TVRNGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSAS 120
           TVRNGSSLRLHISDAH+ SYKPKVYWKDDGFEEVLFTTP +NGGKGGI LVINDAS+SAS
Sbjct: 61  TVRNGSSLRLHISDAHIPSYKPKVYWKDDGFEEVLFTTPAANGGKGGIGLVINDASNSAS 120

Query: 121 KASLLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKP 180
           K SLLP+ DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSESMATAVIVKNTGPK 
Sbjct: 121 KPSLLPTSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESMATAVIVKNTGPKL 180

Query: 181 LTLKSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSS 240
           L+L SAILSHFKFKRRDGAAIQGL+GCSYCS PPLSSPFEILSPTEAMKAEDPDWFSF S
Sbjct: 181 LSLNSAILSHFKFKRRDGAAIQGLEGCSYCSLPPLSSPFEILSPTEAMKAEDPDWFSFGS 240

Query: 241 EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVI 300
           EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAER KPVYHTTPSKYETLDQGRELFFRVI
Sbjct: 241 EPQEKAGSWSLQDVPFTILKNKFSRVYAAPPAERSKPVYHTTPSKYETLDQGRELFFRVI 300

Query: 301 RMGFEDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           RMGFEDIY+ SPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL
Sbjct: 301 RMGFEDIYVQSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 355

BLAST of Lag0022162 vs. ExPASy TrEMBL
Match: A0A1S3CQ42 (photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502972 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 9.0e-180
Identity = 314/351 (89.46%), Postives = 337/351 (96.01%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVRN 60
           MASLVC PLPKPN++RASSSSS S + PSTADSLNDKFARKG HFH +NG+PT+DLTVRN
Sbjct: 1   MASLVCLPLPKPNLIRASSSSS-STSFPSTADSLNDKFARKGFHFHDSNGVPTLDLTVRN 60

Query: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKASL 120
           GSSLRLHISDAHLTSYKPKVYWKDDGFEE+LFTTPT+   KGGIALVI+DASDS+SK+SL
Sbjct: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEILFTTPTA---KGGIALVIDDASDSSSKSSL 120

Query: 121 LPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTLK 180
           LPS DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSES+ATAVIVKNTG KPLTLK
Sbjct: 121 LPSSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESLATAVIVKNTGLKPLTLK 180

Query: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQE 240
           SAILSHFKFK+RDGAAIQGLQGCSYCSHPPLSSPFEILSP+EAMK E+PDWFSF SEPQ 
Sbjct: 181 SAILSHFKFKQRDGAAIQGLQGCSYCSHPPLSSPFEILSPSEAMKVEEPDWFSFGSEPQG 240

Query: 241 KAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMGF 300
           KAGSWSLQDVPFT+LKNKFSRVYAAPPAERLKP+YHTTPSKYETLDQGRELFFRVIRMGF
Sbjct: 241 KAGSWSLQDVPFTMLKNKFSRVYAAPPAERLKPIYHTTPSKYETLDQGRELFFRVIRMGF 300

Query: 301 EDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           EDIY+SSPGSLS+KFGKD+FICTGPASMLVPVTVKPGEQW+GAQVIEHDNL
Sbjct: 301 EDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWKGAQVIEHDNL 347

BLAST of Lag0022162 vs. ExPASy TrEMBL
Match: A0A0A0LCL5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843790 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 4.5e-179
Identity = 313/351 (89.17%), Postives = 333/351 (94.87%), Query Frame = 0

Query: 1   MASLVCFPLPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVRN 60
           MASLVC PLPKPN++RASSSSS S + PST DSLNDKF RKG HFH +NG+PT+DLTVRN
Sbjct: 1   MASLVCLPLPKPNLIRASSSSSSSTSFPSTVDSLNDKFGRKGFHFHDSNGVPTLDLTVRN 60

Query: 61  GSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKASL 120
           GSSLRL ISDAHLTSYKPKVYWKDDGFEEVLFTTPT+   KGGIALVI+DASDS+SK+SL
Sbjct: 61  GSSLRLRISDAHLTSYKPKVYWKDDGFEEVLFTTPTA---KGGIALVIDDASDSSSKSSL 120

Query: 121 LPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTLK 180
           LPS DWTVKDVDSDAIDALQVELSCS+KQ+EITYIVTLYSES+ATAVIVKNTG KPLTLK
Sbjct: 121 LPSSDWTVKDVDSDAIDALQVELSCSSKQMEITYIVTLYSESLATAVIVKNTGLKPLTLK 180

Query: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQE 240
           SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSP+EAMK E+ DWFSF SEPQ 
Sbjct: 181 SAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPSEAMKVEESDWFSFGSEPQG 240

Query: 241 KAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMGF 300
           KAGSWSLQDVPFT+LKNKFSRVYAAPPAERLKP+YHTTPSKYETLDQGRELFFRVIRMGF
Sbjct: 241 KAGSWSLQDVPFTMLKNKFSRVYAAPPAERLKPIYHTTPSKYETLDQGRELFFRVIRMGF 300

Query: 301 EDIYISSPGSLSDKFGKDYFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           EDIY+SSPGSLS+KFGKD+FICTGPASMLVPVTVKPGEQWRGAQVIEHDNL
Sbjct: 301 EDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 348

BLAST of Lag0022162 vs. TAIR 10
Match: AT1G64770.1 (NDH-dependent cyclic electron flow 1 )

HSP 1 Score: 427.2 bits (1097), Expect = 2.3e-119
Identity = 215/353 (60.91%), Postives = 270/353 (76.49%), Query Frame = 0

Query: 1   MASLVCFP-LPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVR 60
           MASL+ F  LPKP  VR SS S+P   + +T + L DKF RKGI F  +N IP V+L VR
Sbjct: 1   MASLISFSLLPKPKAVR-SSISAPQTQTINT-EKLEDKFGRKGIKFSESNNIPMVELKVR 60

Query: 61  NGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKAS 120
           NGSSL+L +SDAH+ SYKPKVYWKD+GFEEVL+T    +  +GG+ +VI +  +    +S
Sbjct: 61  NGSSLKLSLSDAHVLSYKPKVYWKDEGFEEVLYTV-DGDESRGGVGVVIVNGEEPKGGSS 120

Query: 121 LLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTL 180
           ++   DW+VKD DSDAIDALQ+ELSC+   ++ITYIV+LY  SMATA++VKN G KP+TL
Sbjct: 121 VISGCDWSVKDTDSDAIDALQIELSCTAGVLDITYIVSLYPVSMATALVVKNNGRKPVTL 180

Query: 181 KSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQ 240
           K  I+S+ +FK+R GA IQGL+GCSYC +PPLSSPFE+LSP+EAMKAE   W  F SE  
Sbjct: 181 KPGIMSYLRFKKRSGAGIQGLKGCSYCPNPPLSSPFELLSPSEAMKAESSGW--FGSEEG 240

Query: 241 EKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRELFFRVIRMG 300
           EK G W+++D   T+L+ K SR+Y APPAERLK VY+T PSK+ET+DQGR LFFR+IR+G
Sbjct: 241 EKPGIWAVEDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQGRGLFFRMIRIG 300

Query: 301 FEDIYISSPGSLSDKFGKD-YFICTGPASMLVPVTVKPGEQWRGAQVIEHDNL 352
           FE++Y+ SPGS+ DK+GK  YF+CTGP SMLVPV V  GE WRGA VIEHDNL
Sbjct: 301 FEEMYVGSPGSMWDKYGKQHYFVCTGPTSMLVPVDVASGETWRGAMVIEHDNL 348

BLAST of Lag0022162 vs. TAIR 10
Match: AT1G64770.3 (NDH-dependent cyclic electron flow 1 )

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-89
Identity = 169/291 (58.08%), Postives = 218/291 (74.91%), Query Frame = 0

Query: 1   MASLVCFP-LPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVR 60
           MASL+ F  LPKP  VR SS S+P   + +T + L DKF RKGI F  +N IP V+L VR
Sbjct: 1   MASLISFSLLPKPKAVR-SSISAPQTQTINT-EKLEDKFGRKGIKFSESNNIPMVELKVR 60

Query: 61  NGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKAS 120
           NGSSL+L +SDAH+ SYKPKVYWKD+GFEEVL+T    +  +GG+ +VI +  +    +S
Sbjct: 61  NGSSLKLSLSDAHVLSYKPKVYWKDEGFEEVLYTV-DGDESRGGVGVVIVNGEEPKGGSS 120

Query: 121 LLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTL 180
           ++   DW+VKD DSDAIDALQ+ELSC+   ++ITYIV+LY  SMATA++VKN G KP+TL
Sbjct: 121 VISGCDWSVKDTDSDAIDALQIELSCTAGVLDITYIVSLYPVSMATALVVKNNGRKPVTL 180

Query: 181 KSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQ 240
           K  I+S+ +FK+R GA IQGL+GCSYC +PPLSSPFE+LSP+EAMKAE   W  F SE  
Sbjct: 181 KPGIMSYLRFKKRSGAGIQGLKGCSYCPNPPLSSPFELLSPSEAMKAESSGW--FGSEEG 240

Query: 241 EKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQGRE 291
           EK G W+++D   T+L+ K SR+Y APPAERLK VY+T PSK+ET+DQ ++
Sbjct: 241 EKPGIWAVEDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQKKK 286

BLAST of Lag0022162 vs. TAIR 10
Match: AT1G64770.2 (NDH-dependent cyclic electron flow 1 )

HSP 1 Score: 327.4 bits (838), Expect = 2.5e-89
Identity = 169/288 (58.68%), Postives = 216/288 (75.00%), Query Frame = 0

Query: 1   MASLVCFP-LPKPNIVRASSSSSPSATSPSTADSLNDKFARKGIHFHHANGIPTVDLTVR 60
           MASL+ F  LPKP  VR SS S+P   + +T + L DKF RKGI F  +N IP V+L VR
Sbjct: 1   MASLISFSLLPKPKAVR-SSISAPQTQTINT-EKLEDKFGRKGIKFSESNNIPMVELKVR 60

Query: 61  NGSSLRLHISDAHLTSYKPKVYWKDDGFEEVLFTTPTSNGGKGGIALVINDASDSASKAS 120
           NGSSL+L +SDAH+ SYKPKVYWKD+GFEEVL+T    +  +GG+ +VI +  +    +S
Sbjct: 61  NGSSLKLSLSDAHVLSYKPKVYWKDEGFEEVLYTV-DGDESRGGVGVVIVNGEEPKGGSS 120

Query: 121 LLPSFDWTVKDVDSDAIDALQVELSCSNKQVEITYIVTLYSESMATAVIVKNTGPKPLTL 180
           ++   DW+VKD DSDAIDALQ+ELSC+   ++ITYIV+LY  SMATA++VKN G KP+TL
Sbjct: 121 VISGCDWSVKDTDSDAIDALQIELSCTAGVLDITYIVSLYPVSMATALVVKNNGRKPVTL 180

Query: 181 KSAILSHFKFKRRDGAAIQGLQGCSYCSHPPLSSPFEILSPTEAMKAEDPDWFSFSSEPQ 240
           K  I+S+ +FK+R GA IQGL+GCSYC +PPLSSPFE+LSP+EAMKAE   W  F SE  
Sbjct: 181 KPGIMSYLRFKKRSGAGIQGLKGCSYCPNPPLSSPFELLSPSEAMKAESSGW--FGSEEG 240

Query: 241 EKAGSWSLQDVPFTILKNKFSRVYAAPPAERLKPVYHTTPSKYETLDQ 288
           EK G W+++D   T+L+ K SR+Y APPAERLK VY+T PSK+ET+DQ
Sbjct: 241 EKPGIWAVEDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQ 283

BLAST of Lag0022162 vs. TAIR 10
Match: AT3G03980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 1.0e-74
Identity = 157/273 (57.51%), Postives = 191/273 (69.96%), Query Frame = 0

Query: 367 VSTMASESGPVVAALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQV 426
           +ST +S S P    LPL  RVAIVTG+SRGIGRAIA+HLA LGAR+VINY S +A A++V
Sbjct: 1   MSTHSSISQP---PLPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 60

Query: 427 AAEIN-----SSSAAGCQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDP 486
           A+EIN             RAI  +A+VS+P QVKS+FDAAE AF + VHILVNSAGI DP
Sbjct: 61  ASEINDFPVREEITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDP 120

Query: 487 TYPCIADTSLEIFDQIF-----------REAATRVKHGGGGRIILISSTAVAAPTAGLGA 546
            YP IADTS+E FD  F           +EAA R+K GGGGRIIL++S+   +   G GA
Sbjct: 121 KYPTIADTSVEDFDHTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGA 180

Query: 547 YTASKAAVEAMTKVAAKELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRL 606
           Y ASKAAVE M K+ AKEL+G+GIT NC+APG  ATEMF+ G   E V+K+  + P GR+
Sbjct: 181 YAASKAAVETMVKILAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRV 240

Query: 607 GDPKDVASFVGFLASDDGEWINGQVVLVNGGIV 624
           G+ KDV   VGFLA D GEW+NGQ++ VNGG V
Sbjct: 241 GEAKDVVPLVGFLAGDGGEWVNGQIIPVNGGYV 270

BLAST of Lag0022162 vs. TAIR 10
Match: AT4G13180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 3.9e-74
Identity = 145/256 (56.64%), Postives = 183/256 (71.48%), Query Frame = 0

Query: 379 AALPLQDRVAIVTGASRGIGRAIALHLAALGARLVINYVSDSAKADQVAAEINSSSAAGC 438
           ++LPL  RVAIVTGA+RG+GR IA+HL +LGAR+ INYVS S+KA+ + +E+N SS    
Sbjct: 9   SSLPLAGRVAIVTGATRGMGREIAIHLHSLGARVTINYVSSSSKAELLVSELNDSSQ--L 68

Query: 439 QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPCIADTSLEIFDQIF 498
           + AIA +ADVSDP+Q+ +LFD  EQ FGS+VHI+VN AG+ DP YP +++T+LE FD  F
Sbjct: 69  KSAIAVKADVSDPDQINNLFDQTEQEFGSKVHIVVNCAGVLDPKYPSLSETTLEDFDNTF 128

Query: 499 -----------REAATRVKHGGGGRIILISSTAVAAPTAGLGAYTASKAAVEAMTKVAAK 558
                      +EAA RV  GGGGRII++S++ V     G G Y ASKAAVE M KV AK
Sbjct: 129 TINTRGSFLCCKEAAKRVMRGGGGRIIMMSTSMVGGLAPGYGVYAASKAAVETMVKVLAK 188

Query: 559 ELRGSGITVNCIAPGATATEMFYRGMDEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDD 618
           EL+GS IT NC+APG  ATEMFY G  +E VK +   CP+GR+G+ KD+   VGFLA D 
Sbjct: 189 ELKGSRITANCVAPGPVATEMFYAGKSDETVKMLAGACPMGRIGESKDITEIVGFLAGDG 248

Query: 619 GEWINGQVVLVNGGIV 624
           GEWINGQV+  NGG V
Sbjct: 249 GEWINGQVIRANGGFV 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0051280.14.6e-30385.35photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK2998... [more]
KGN59874.21.5e-30185.20hypothetical protein Csa_001420 [Cucumis sativus][more]
XP_022972247.12.4e-18290.50photosynthetic NDH subunit of subcomplex B 2, chloroplastic [Cucurbita maxima][more]
XP_022932774.16.8e-18290.99photosynthetic NDH subunit of subcomplex B 2, chloroplastic [Cucurbita moschata][more]
XP_023540761.12.0e-18190.70photosynthetic NDH subunit of subcomplex B 2, chloroplastic [Cucurbita pepo subs... [more]
Match NameE-valueIdentityDescription
Q94AQ83.3e-11860.91Photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Arabidopsis thali... [more]
Q9SQR41.4e-7357.51NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis th... [more]
Q9SQR24.8e-6954.28NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q086327.9e-6456.25Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1[more]
P518311.7e-3438.803-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Bacillus subtilis (strain 168... [more]
Match NameE-valueIdentityDescription
A0A5A7U7W32.2e-30385.35Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1I4B21.1e-18290.50photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Cucurbita maxima ... [more]
A0A6J1EXZ13.3e-18290.99photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Cucurbita moschat... [more]
A0A1S3CQ429.0e-18089.46photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Cucumis melo OX=3... [more]
A0A0A0LCL54.5e-17989.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843790 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G64770.12.3e-11960.91NDH-dependent cyclic electron flow 1 [more]
AT1G64770.31.5e-8958.08NDH-dependent cyclic electron flow 1 [more]
AT1G64770.22.5e-8958.68NDH-dependent cyclic electron flow 1 [more]
AT3G03980.11.0e-7457.51NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G13180.13.9e-7456.64NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 467..478
score: 42.09
coord: 387..404
score: 54.68
coord: 505..521
score: 32.88
coord: 552..569
score: 43.18
coord: 586..606
score: 43.75
coord: 531..550
score: 41.37
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 531..550
score: 40.62
coord: 467..478
score: 45.56
coord: 511..519
score: 49.84
NoneNo IPR availableSMARTSM00822PKS_KRcoord: 386..562
e-value: 6.0E-4
score: -45.1
NoneNo IPR availableGENE3D3.40.50.720coord: 359..622
e-value: 1.6E-70
score: 239.4
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 392..622
e-value: 3.4E-49
score: 167.5
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 389..575
e-value: 1.3E-8
score: 32.2
NoneNo IPR availablePIRSRPIRSR629511-2PIRSR629511-2coord: 376..621
e-value: 9.4E-29
score: 98.1
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 383..621
e-value: 1.1E-8
score: 32.4
NoneNo IPR availablePANTHERPTHR11122APOSPORY-ASSOCIATED PROTEIN C-RELATEDcoord: 15..351
NoneNo IPR availablePANTHERPTHR11122:SF18PHOTOSYNTHETIC NDH SUBUNIT OF SUBCOMPLEX B 2, CHLOROPLASTICcoord: 15..351
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 51..356
e-value: 5.1E-25
score: 90.1
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 518..546
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 384..622
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 43..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0022162.1Lag0022162.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006303 double-strand break repair via nonhomologous end joining
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0097552 mitochondrial double-strand break repair via homologous recombination
biological_process GO:0007095 mitotic G2 DNA damage checkpoint signaling
biological_process GO:0009773 photosynthetic electron transport in photosystem I
biological_process GO:0000723 telomere maintenance
cellular_component GO:0035861 site of double-strand break
cellular_component GO:0030870 Mre11 complex
cellular_component GO:0010598 NAD(P)H dehydrogenase complex (plastoquinone)
cellular_component GO:0009535 chloroplast thylakoid membrane
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0047938 glucose-6-phosphate 1-epimerase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity
molecular_function GO:0003824 catalytic activity