Lag0022121 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0022121
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionHNHc domain-containing protein
Locationchr7: 18889294 .. 18894263 (+)
RNA-Seq ExpressionLag0022121
SyntenyLag0022121
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCAATTCACCGCACACAGTCGGGTTAAGTTGCTGCTCAACGGAGACGGAGTGCCATTTGAAGATCGATTGAGTTACAAGCTCAGATCAGTGCGAACCCTTAAGCGTAGGATCCCTTTATCAGGTGCCTCCTCCTCTGCACTTTCCCCTTCTGCATCCTCTGCTTCAGCTTTGAGGAAATCCGCTCTGCGTGTTAGTGTGAGGGGTGAGAGCGTTAGCGGTGACGACGCCATTGTTGATCTTAACTACGATTACGAGTTTGAGAGTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGTGAATTCACCTTCATACCCGCTTCGGTGTTTTTAATCGATGAGCTCTTCTTGAGTGATGTGCGACTCTGTTATCTGTTAGAGTGTTAATAGGGAAATAATTGGAATGTTGTTAGGACATTAATCGTCGCATAGTTGTAGTTAGTTAGGAAGTTAGTTAGAGTGCATGGCTATAAATAGCTGGAGTTGGGAGTTTAAGAATTAAGCCTAATTTTAGGAATTGGTCTAATATAGGAAAAATTAATATAGGACTTGGGAGAGATAACCGTATCCGCTATTGTTTTTGCAGTGTCTTCGTTTTGTCATTTTCGTATAACTGGGATCCTAACAGGAAAAATCTTAACGCTTTCCGAGTACCTTTTAACTTGTCCCTTTTTTCTTTTGGGTGAAATTATGGGTAGAGTTCGACTTCTAGACATATTTATTATCAATATTGCATTTTCGGTTAGCTGTTTTACTTGGCCTTACTGTGTTTAAACGAATAAAAAATTTCCACATTAGTTAATGTTGAAGCAAATGCTAGCTTGGAGTAGGTCAATGATTTAACAAAATTCTGATATTGGAGCTTAAAGTGGGGCCAGGCAAAAGCATTAACACAAGAGGAAACATGAAGCCAGCCAGTTTATCAAATTGATTATTCTTGATTCTGTCCCAACGATCAGGAGCTAATATATCATGTATTTGAAGTATTGCAGATCATTTGTCCTTCATGTTGGTATAGAATTTCAATTACTATTATGAAATACTTTTACGCGTTTGAGTTCGATTAGAAGTTTCTAGGAGGCACCATAAGCAGAGCTTGTGTTTTTCCTATTTATGGCCGAGGTTTAAAAGTAGGCAGCCCAGAAGTGGATACATATCCAGCTCAACGTGTATACAGTTGAAAGCAATATGAAGGAAAAGAAGTTGACTAAAAGAATAAAATATTGAGTGTGTTGTCTATCCAATACATCTGCTCGATCAGGACTGATCTTGCATGTCGAGCTTTGTCTATGAACGACCTCTTGGCAAAGCCTTTTTTTTTTTCTTTAAAAAGATCAGCCATCTGAGCTTTTGCAAGAGAATGCAGTGAATCTTGACTTCTATATTCTTGAAATGCTCTCATATAAAGTGCCTGTCAACGCCTTTATATTTGTGTCCTGGAGTAAAGAACAGGATTTGATGCTAATGATGCAACCTCCATGTTATTGAAGATATCAATAGCATGAGTAAGGTTGCCATCCTAATCATTTTCTTAAAAATGTATGGCTATGGAACATGAGGAACATGATTCCAATGGAACAACCTTTGATAGTTTGATTCAATGTTGAGGCGTTGGATAAGGGCGTGGATAAGGGTTTTTTAAGCCTTTTCATTACCTATAATCAGTTTTCAGGGCCTCAGTGTTAATTTCTGATCTTTGGATCGTTAAGTTCACTGATCATATGCTTGTTGGCCAACAGCCCAAATACCATACTGAACTTGATACGGTCCCCTGGATGCAAAATAGCTGCCAACCAGAACGATCTTGCATAGGTGTAAAGCAATATGAAGAAAGAAAATAAGTTAGTTAAAAGAATATGGGTTGCAGTTGCACCAACCCCCACCTGCAAGCCAGATTTATCTTGTGCACTGAGGTTGTCAAATGTAAACAAAGAAAGTCACCTCTTAGCTTTACTGAAAGTATGGAGCCTTTGGTCTTTCCAGGGAACATAGCAAATTTTACCTTTCCATTTATCCTTCCAGTAGCATAAATTTTTAAATCCAATTATTTGATACGACTGCCATTTTGACTTTAAGAGCCAATTCGTTCTAGCACCATACTTCTGGATTCTTGCAAATGTTGATACAATGTAAACAAGGGATGACTGAAGTCGTCTTTAGATATTGCGTCATTCTCAACATTTCAGCTATTTTAGATAAGCATGGAGCCACGAATGATATTTTGTGTCCATTCCTGCATGTGGTACTACGCACTGAAGAGAATAAACAAACACAAACAAACTCCACTTTTTCCAGTACTTTCTGCTTTAAAATTTTTTGTTCATTTCCAACTAGTTAGTCAAACAAATACTTGACTCGATTTCTTTATGTGGAATGGATTGACCTACACTTTTATGTGTATACCTGTTTCATATTGACTTTATGTCTCCATATTATTTGTATGACTGGAAAGATTTGCAATTTATCTTTGGCTATTGATTTCCTTGCTTCAGGCCAGTCAACGTTGTTTGTTGGAAGCGTGCAATTTGTTTGGAGTTCATGGAGAAGGTTTGACTAATCTCTTTTACGTATTATGTATAAAAATAAAGATAATATATAGTTCTTAGTGTAACAATTAATTGGTTTCATGTTTCTTATTGAAAATATGTCTAGAAGATGAGGAAGTTATCATCTTTTGGAAAATTTTGAGTTCATAGTCAGGGGAAATATAGTATGTCTGTCCAATCAGTGAGAGTGAAAAGCCAAATGGGAGTGGCTAGATGCTTTGGTATTTAAATTGTCACTCTCTGCAATTTATATATCTTTTCAGAAAGTCTGTCAATTTTAAATCTTGATTACAGATTTCAAAAGGTTTAAATAACGATTTGGTCTTATCAATGCATGTTTCGATATGCCTTTATCTCAAACCTTTTTGGCTTTTCCAGGCTGATGTATTGGAATACTACGACCAGACTGTGAGTTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTAAGGGTATGCCTAAACCATGTATCTAGTTCATTCATGGGTTTTTTTCGTAGGATTGTTACATGTTATCAGTCTTCATTTCCATAAGAAGTTCAAGGCTGAATTCATCTTTTTGACTTGAATATTTCCTTGTCAAAGGCCCTTGAACTAACTATATCGTCTCTCTTTTCAGGTTCCCCATTTATTGCAAGTTGTAAAGAGAAGAAGAATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTAAGTTGATAAATATTCATAATTGACTAGTTTTATTCAATTTATCATTTAAAATTGATATGGTAGAAGTTATTTTGAGATGGTTTGCTAATTTTATGATGGGGGAGCATGTTTTGATTATATAATCATTTTAATTGTAGCTTCACTAAACACGCTTTGTTATTTTAATTGTATTGCTATCTTTATGACGGCAGATGGCACACTCCACTTTTTTTTTTTTTTTTTTTTATCTCTTTAATATTAGTGCCCATTGGGTTTGCATTGGCTCTGTCTCAAATCTCGTGCCCGAAGCGTCCATCCCAATTATAAAGGGTAACGAGAAATCTGGTAAGGCCAACATATGCAGTGCTATCATAGCCTTAATAGCCTCTGAATTGCATGTGAGCTATCCCTTTTGAGCCTTTTGTAAGGGGAGAAGAGGTAAGCATTATAAGTTGTCATGAAGCAACCTTATTACCCTGTCATACCCAGATAACCGAATAATTCTTTCAAAGATTTCAGAGATGGCCACTGTATGGCATGCACGTTACTGGTCCTTTACACCTTTGGATGAGATCCAAACGAAGCCTTATTATCTAAAATGGCTTTGGAGGTTCTTGACTGAGTTTGATTAGTTTTGGTAGATTTAGGTTTATTAAATTCTTATCACTACTTTCAATAGGAAGGTAGCTAGACTGAATATTGAAATGAGGGATGATATTGCAAGGATCTGAATGGAAAATCTTTTGCTCCAATTCTTTTTAGTTCTTGTTATTTTCTGGCAAAGGGTGAATACTAATTTCATCCAAGACAACTTTTATGTGCTGTCTTTTCTTTTTCTTTATTTTGTTTGTTGCCTCAAATGGGTCTTTTATCAACAGGTATTGTTCATCACATGAGAGTTTGACCATTGACCATGTTTTGCCCATATGCCGGGGTGGAGAATGGACATGGGAAAATCTGGTGAGTTATCCAGTAGATATTAATATTATATTACAAATGCTCAACTGATCCTGATTCCAAAACAGAGAGATGTCCAGAGATTTTCTTGGTAATAAAATAGAACACAGGAGTTTAAAGGCCAAATTGGCAAAGCATCTTCTGTTTCATTTGAACAATTCAGCTGTTACATTGTTAATTCTGAGACCATATAAATATGTCGAGTTCCAAAAACTGTTCTGTGGTTATAGCTTTTGGGGATTGGATGTTCGGAAAATGGGACTTCTAAAAAGGAGTTGCTATGATGCTTACCAACAACTCTTCATTTATGAAATTAGTTCACTGTTTTCTGTTTTCTCTAATCTCTTATGTTGTAATAGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGTCAGAAAACTGTAGAAGAAGCAAATATGAAGCTGAAAAAGACTCCCAAGGTAATATATTATGTTTTGTTTATAGTGAGAAATTGAATTAAATATTATGTTTAATACAGAAAGAAACTTCTGATTTTCTTTTCTTTCGTGTGTGTGTGGGTGAAAATCCGTCTGCTATTCTTCCTTATTCTAATGTTTCATCAGGTCTCACTGCATCTAGTTCAGGCTTATTGATTTTTACCTCAAAATTTTCAGGCCCCAAAAGATTATGATATACTTGCCATTCCTCTAACGAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGGACCCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGA

mRNA sequence

ATGGCCCAATTCACCGCACACAGTCGGGTTAAGTTGCTGCTCAACGGAGACGGAGTGCCATTTGAAGATCGATTGAGTTACAAGCTCAGATCAGTGCGAACCCTTAAGCGTAGGATCCCTTTATCAGGTGCCTCCTCCTCTGCACTTTCCCCTTCTGCATCCTCTGCTTCAGCTTTGAGGAAATCCGCTCTGCGTGTTAGTGTGAGGGGTGAGAGCGTTAGCGGTGACGACGCCATTGTTGATCTTAACTACGATTACGAGTTTGAGAGTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGCCAGTCAACGTTGTTTGTTGGAAGCGTGCAATTTGTTTGGAGTTCATGGAGAAGGCTGATGTATTGGAATACTACGACCAGACTGTGAGTTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTAAGGGTTCCCCATTTATTGCAAGTTGTAAAGAGAAGAAGAATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTATTGTTCATCACATGAGAGTTTGACCATTGACCATGTTTTGCCCATATGCCGGGGTGGAGAATGGACATGGGAAAATCTGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGTCAGAAAACTGTAGAAGAAGCAAATATGAAGCTGAAAAAGACTCCCAAGGCCCCAAAAGATTATGATATACTTGCCATTCCTCTAACGAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGGACCCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGA

Coding sequence (CDS)

ATGGCCCAATTCACCGCACACAGTCGGGTTAAGTTGCTGCTCAACGGAGACGGAGTGCCATTTGAAGATCGATTGAGTTACAAGCTCAGATCAGTGCGAACCCTTAAGCGTAGGATCCCTTTATCAGGTGCCTCCTCCTCTGCACTTTCCCCTTCTGCATCCTCTGCTTCAGCTTTGAGGAAATCCGCTCTGCGTGTTAGTGTGAGGGGTGAGAGCGTTAGCGGTGACGACGCCATTGTTGATCTTAACTACGATTACGAGTTTGAGAGTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGCCAGTCAACGTTGTTTGTTGGAAGCGTGCAATTTGTTTGGAGTTCATGGAGAAGGCTGATGTATTGGAATACTACGACCAGACTGTGAGTTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTAAGGGTTCCCCATTTATTGCAAGTTGTAAAGAGAAGAAGAATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTATTGTTCATCACATGAGAGTTTGACCATTGACCATGTTTTGCCCATATGCCGGGGTGGAGAATGGACATGGGAAAATCTGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGTCAGAAAACTGTAGAAGAAGCAAATATGAAGCTGAAAAAGACTCCCAAGGCCCCAAAAGATTATGATATACTTGCCATTCCTCTAACGAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGGACCCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGA

Protein sequence

MAQFTAHSRVKLLLNGDGVPFEDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSASALRKSALRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Homology
BLAST of Lag0022121 vs. NCBI nr
Match: XP_022932702.1 (uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022932703.1 uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022972084.1 uncharacterized protein LOC111470725 [Cucurbita maxima] >XP_023539770.1 uncharacterized protein LOC111800353 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.8 bits (1309), Expect = 2.8e-140
Identity = 257/277 (92.78%), Postives = 265/277 (95.67%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  +KLRSVRTLKRR PLSGASS+ LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  SALRKSALRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
           SALRKSA RV VRGESVSGDDAI+D++YDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of Lag0022121 vs. NCBI nr
Match: KAG6597719.1 (hypothetical protein SDJN03_10899, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029166.1 hypothetical protein SDJN02_10351, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.5 bits (1303), Expect = 1.4e-139
Identity = 256/277 (92.42%), Postives = 264/277 (95.31%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR   KLRS+RTLKRR PLSGASS+ LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRQKLRSLRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  SALRKSALRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
           SALRKSA RV VRGESVSGDDAI+D++YDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of Lag0022121 vs. NCBI nr
Match: XP_038905692.1 (uncharacterized protein LOC120091663 [Benincasa hispida])

HSP 1 Score: 496.1 bits (1276), Expect = 1.9e-136
Identity = 256/282 (90.78%), Postives = 261/282 (92.55%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  YKLR VRTLKRRIPLSG      SPS SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRFRYKLRLVRTLKRRIPLSGP-----SPSTSSA 60

Query: 61  SALRKSA-----LRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVV 120
           SALRKSA     +RV VRGESVSGDDAIVDL+YDYEFE+DDLACFRGLVLDISYRPVNVV
Sbjct: 61  SALRKSAQHAAEVRVGVRGESVSGDDAIVDLDYDYEFETDDLACFRGLVLDISYRPVNVV 120

Query: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180
           CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI
Sbjct: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180

Query: 181 LYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240
           LYRDNYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACV+CNSKKGQKTVEEANMKLK
Sbjct: 181 LYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVQCNSKKGQKTVEEANMKLK 240

Query: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of Lag0022121 vs. NCBI nr
Match: XP_022152374.1 (uncharacterized protein LOC111020122 [Momordica charantia])

HSP 1 Score: 469.5 bits (1207), Expect = 1.9e-128
Identity = 247/282 (87.59%), Postives = 257/282 (91.13%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFT  +RVKLLLNGDGVPF    +DRL YKLRSV    RRIPLS A+S+ +SPSASSA
Sbjct: 1   MAQFTTLNRVKLLLNGDGVPFGSESKDRLRYKLRSV----RRIPLS-AASTGISPSASSA 60

Query: 61  SALRKSA-----LRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVV 120
           SALRKSA     +RV VR ESVS D AIV L+ DYEFESDDLACFRGLVLDISYRPVNVV
Sbjct: 61  SALRKSAQHVAEMRVGVRDESVSDDGAIVGLDCDYEFESDDLACFRGLVLDISYRPVNVV 120

Query: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180
           CWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI
Sbjct: 121 CWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180

Query: 181 LYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240
           LYRDNYTCQYCSSH+SLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK
Sbjct: 181 LYRDNYTCQYCSSHDSLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240

Query: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS+EQ
Sbjct: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSNEQ 277

BLAST of Lag0022121 vs. NCBI nr
Match: XP_008465340.1 (PREDICTED: uncharacterized protein LOC103502982 [Cucumis melo] >KAA0051262.1 HNH endonuclease [Cucumis melo var. makuwa] >TYK29998.1 HNH endonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 464.5 bits (1194), Expect = 6.1e-127
Identity = 241/278 (86.69%), Postives = 248/278 (89.21%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVP----FEDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDG+P     +DR  YKLRSVR   RR PLS  SSS  S SA   
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRA--RRFPLSAPSSSTSSTSALRK 60

Query: 61  SALRKSALRVSVRGESVS-GDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKR 120
           S    + +RV VR ESV+ GDDAIV  +YDYEFESDDLACFRGLVLDISYRPVNVVCWKR
Sbjct: 61  STQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVVCWKR 120

Query: 121 AICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD 180
           AICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD
Sbjct: 121 AICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD 180

Query: 181 NYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK 240
           NYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK
Sbjct: 181 NYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK 240

Query: 241 APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

BLAST of Lag0022121 vs. ExPASy TrEMBL
Match: A0A6J1F2H9 (uncharacterized protein LOC111439170 OS=Cucurbita moschata OX=3662 GN=LOC111439170 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.4e-140
Identity = 257/277 (92.78%), Postives = 265/277 (95.67%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  +KLRSVRTLKRR PLSGASS+ LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  SALRKSALRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
           SALRKSA RV VRGESVSGDDAI+D++YDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of Lag0022121 vs. ExPASy TrEMBL
Match: A0A6J1IAG4 (uncharacterized protein LOC111470725 OS=Cucurbita maxima OX=3661 GN=LOC111470725 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.4e-140
Identity = 257/277 (92.78%), Postives = 265/277 (95.67%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  +KLRSVRTLKRR PLSGASS+ LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  SALRKSALRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
           SALRKSA RV VRGESVSGDDAI+D++YDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

BLAST of Lag0022121 vs. ExPASy TrEMBL
Match: A0A6J1DFU6 (uncharacterized protein LOC111020122 OS=Momordica charantia OX=3673 GN=LOC111020122 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 9.2e-129
Identity = 247/282 (87.59%), Postives = 257/282 (91.13%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFT  +RVKLLLNGDGVPF    +DRL YKLRSV    RRIPLS A+S+ +SPSASSA
Sbjct: 1   MAQFTTLNRVKLLLNGDGVPFGSESKDRLRYKLRSV----RRIPLS-AASTGISPSASSA 60

Query: 61  SALRKSA-----LRVSVRGESVSGDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVV 120
           SALRKSA     +RV VR ESVS D AIV L+ DYEFESDDLACFRGLVLDISYRPVNVV
Sbjct: 61  SALRKSAQHVAEMRVGVRDESVSDDGAIVGLDCDYEFESDDLACFRGLVLDISYRPVNVV 120

Query: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180
           CWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI
Sbjct: 121 CWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180

Query: 181 LYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240
           LYRDNYTCQYCSSH+SLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK
Sbjct: 181 LYRDNYTCQYCSSHDSLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240

Query: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS+EQ
Sbjct: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSNEQ 277

BLAST of Lag0022121 vs. ExPASy TrEMBL
Match: A0A5D3E2H2 (HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00280 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.0e-127
Identity = 241/278 (86.69%), Postives = 248/278 (89.21%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVP----FEDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDG+P     +DR  YKLRSVR   RR PLS  SSS  S SA   
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRA--RRFPLSAPSSSTSSTSALRK 60

Query: 61  SALRKSALRVSVRGESVS-GDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKR 120
           S    + +RV VR ESV+ GDDAIV  +YDYEFESDDLACFRGLVLDISYRPVNVVCWKR
Sbjct: 61  STQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVVCWKR 120

Query: 121 AICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD 180
           AICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD
Sbjct: 121 AICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD 180

Query: 181 NYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK 240
           NYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK
Sbjct: 181 NYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK 240

Query: 241 APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

BLAST of Lag0022121 vs. ExPASy TrEMBL
Match: A0A1S3CNK0 (uncharacterized protein LOC103502982 OS=Cucumis melo OX=3656 GN=LOC103502982 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.0e-127
Identity = 241/278 (86.69%), Postives = 248/278 (89.21%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVP----FEDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSA 60
           MAQFTAHSRVKLLLNGDG+P     +DR  YKLRSVR   RR PLS  SSS  S SA   
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRA--RRFPLSAPSSSTSSTSALRK 60

Query: 61  SALRKSALRVSVRGESVS-GDDAIVDLNYDYEFESDDLACFRGLVLDISYRPVNVVCWKR 120
           S    + +RV VR ESV+ GDDAIV  +YDYEFESDDLACFRGLVLDISYRPVNVVCWKR
Sbjct: 61  STQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVVCWKR 120

Query: 121 AICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD 180
           AICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD
Sbjct: 121 AICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRD 180

Query: 181 NYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK 240
           NYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK
Sbjct: 181 NYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPK 240

Query: 241 APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 274
           APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 APKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

BLAST of Lag0022121 vs. TAIR 10
Match: AT2G23840.1 (HNH endonuclease )

HSP 1 Score: 323.6 bits (828), Expect = 1.6e-88
Identity = 173/280 (61.79%), Postives = 208/280 (74.29%), Query Frame = 0

Query: 1   MAQFTAHSRVKLLLNGDGVPF-EDRLSYKLRSVRTLKRRIPLSGASSSALSPSASSASAL 60
           MA F+A  R+KLL + DG+ F  D      +S+       PL     S L   A   S+ 
Sbjct: 1   MAHFSA--RLKLLSSNDGLSFGVDSRDQFRQSLYMAGGGSPLVPIRMSRLKFCARIFSSY 60

Query: 61  RKSALRVSVRG-ESVSGDDAIVDLNYDYEFESDD--------LACFRGLVLDISYRPVNV 120
                R  +R  ++   +  I + N +++F+ DD        L+CFRGLVLDISYRPVNV
Sbjct: 61  SFPYKRKKIRDLDNTEKNLDIDEDNDEWDFDGDDDGLETDDHLSCFRGLVLDISYRPVNV 120

Query: 121 VCWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKN 180
           VCWKRAICLE+M+KADVLEYYDQTVSSP+GSFYIPAVLRVPHLLQVVKRRR+KNSLSRKN
Sbjct: 121 VCWKRAICLEYMDKADVLEYYDQTVSSPTGSFYIPAVLRVPHLLQVVKRRRVKNSLSRKN 180

Query: 181 ILYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKL 240
           IL RD+YTCQYCSS E+LTIDHV+P+ RGGEWTW+NLVAAC +CNS+KGQKT +EA+MKL
Sbjct: 181 ILLRDDYTCQYCSSRENLTIDHVMPVSRGGEWTWQNLVAACSRCNSRKGQKTADEAHMKL 240

Query: 241 KKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS 271
            K PK PKDYDI+AIPLT+ AI+ML+  KG PEEWRQYL+
Sbjct: 241 LKVPKEPKDYDIVAIPLTNAAIRMLRSNKGMPEEWRQYLA 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022932702.12.8e-14092.78uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022932703.1 unchar... [more]
KAG6597719.11.4e-13992.42hypothetical protein SDJN03_10899, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038905692.11.9e-13690.78uncharacterized protein LOC120091663 [Benincasa hispida][more]
XP_022152374.11.9e-12887.59uncharacterized protein LOC111020122 [Momordica charantia][more]
XP_008465340.16.1e-12786.69PREDICTED: uncharacterized protein LOC103502982 [Cucumis melo] >KAA0051262.1 HNH... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1F2H91.4e-14092.78uncharacterized protein LOC111439170 OS=Cucurbita moschata OX=3662 GN=LOC1114391... [more]
A0A6J1IAG41.4e-14092.78uncharacterized protein LOC111470725 OS=Cucurbita maxima OX=3661 GN=LOC111470725... [more]
A0A6J1DFU69.2e-12987.59uncharacterized protein LOC111020122 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A5D3E2H23.0e-12786.69HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G002... [more]
A0A1S3CNK03.0e-12786.69uncharacterized protein LOC103502982 OS=Cucumis melo OX=3656 GN=LOC103502982 PE=... [more]
Match NameE-valueIdentityDescription
AT2G23840.11.6e-8861.79HNH endonuclease [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003615HNH nucleaseSMARTSM00507HNH_5coord: 166..216
e-value: 6.7E-14
score: 62.2
IPR003615HNH nucleaseCDDcd00085HNHccoord: 168..219
e-value: 6.10305E-13
score: 60.1779
IPR029471HNH endonuclease 5PFAMPF14279HNH_5coord: 179..230
e-value: 3.2E-14
score: 52.5
NoneNo IPR availableGENE3D1.10.30.50coord: 154..243
e-value: 5.6E-23
score: 82.6
NoneNo IPR availablePANTHERPTHR33877:SF2SLL1193 PROTEINcoord: 61..271
NoneNo IPR availablePANTHERPTHR33877SLL1193 PROTEINcoord: 61..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0022121.1Lag0022121.1mRNA