Lag0022052 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0022052
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDUF4228 domain-containing protein
Locationchr7: 17131372 .. 17132205 (+)
RNA-Seq ExpressionLag0022052
SyntenyLag0022052
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTCTAACTGTTCAGAAGAATATGGTTTCCTGGTCTTGTTTCCATTTGGTCAAGAACGGGATTCCCGATTCCCCCATCGCCAACATCAAGCTCATTCATTCCGACGGCCTCGTCCGAATCTACCATCGCCCCATCCATGTTTCCGAGGTCTTGCTTGAATTCCCCAAGCATTCCGTCTGCCGATCCGATTCCTTTTACATCGGCCAGAAAATCCCTCCTCTCGCCGAACACGACCAGCTCCAGCTCGGCCACAAATACTTCCTTCTCCCCAACCCATTCTTCCACTCTGCTCTCTCTTTCGTCACCATCGCTTCTTTCTTTTCCTCTTCCAACGCCAACAACACCACCACCACCACCAACGTCAGATTCATCAACAACGCCGCCGCCTGCCAAAACCCTTTCGACATCCAGCGAACTCCCTCCGGCTGCCTCCGAATCCGCGTCTCCGATGAGTTCATTTCTCAGTTGCTCGAACAGGGCAACCCCAAAATCCAACAACAACAACAACAACAGCAATCCTCATCGTTACCGCTCGGAAAAATCTGCACGACGCCCCAATTGGCCAAAGACTACGCCCAGCTCGTCAGAACGCGCCGATGGAAGCCCAAGCTCGAGACCGTTCCAGAGATCACTAAACCAAAAGGGATTTCGCCGTTTGGGTTGAAGAAGAGGAAGCCCTTTCATTTCCAATTTCAATTGAATCCGCCGCCGTCGCCGCCGCTCAAGTCGGCTGCATCGGCTTGGACCAAATCGGCCTACCGACTTCACCATCTCCGTTCGATTCAGTTTGCTTATAAGCCTAAGATTCGCATCAGATCCAAGGCTTAA

mRNA sequence

ATGAAGCTTCTAACTGTTCAGAAGAATATGGTTTCCTGGTCTTGTTTCCATTTGGTCAAGAACGGGATTCCCGATTCCCCCATCGCCAACATCAAGCTCATTCATTCCGACGGCCTCGTCCGAATCTACCATCGCCCCATCCATGTTTCCGAGGTCTTGCTTGAATTCCCCAAGCATTCCGTCTGCCGATCCGATTCCTTTTACATCGGCCAGAAAATCCCTCCTCTCGCCGAACACGACCAGCTCCAGCTCGGCCACAAATACTTCCTTCTCCCCAACCCATTCTTCCACTCTGCTCTCTCTTTCGTCACCATCGCTTCTTTCTTTTCCTCTTCCAACGCCAACAACACCACCACCACCACCAACGTCAGATTCATCAACAACGCCGCCGCCTGCCAAAACCCTTTCGACATCCAGCGAACTCCCTCCGGCTGCCTCCGAATCCGCGTCTCCGATGAGTTCATTTCTCAGTTGCTCGAACAGGGCAACCCCAAAATCCAACAACAACAACAACAACAGCAATCCTCATCGTTACCGCTCGGAAAAATCTGCACGACGCCCCAATTGGCCAAAGACTACGCCCAGCTCGTCAGAACGCGCCGATGGAAGCCCAAGCTCGAGACCGTTCCAGAGATCACTAAACCAAAAGGGATTTCGCCGTTTGGGTTGAAGAAGAGGAAGCCCTTTCATTTCCAATTTCAATTGAATCCGCCGCCGTCGCCGCCGCTCAAGTCGGCTGCATCGGCTTGGACCAAATCGGCCTACCGACTTCACCATCTCCGTTCGATTCAGTTTGCTTATAAGCCTAAGATTCGCATCAGATCCAAGGCTTAA

Coding sequence (CDS)

ATGAAGCTTCTAACTGTTCAGAAGAATATGGTTTCCTGGTCTTGTTTCCATTTGGTCAAGAACGGGATTCCCGATTCCCCCATCGCCAACATCAAGCTCATTCATTCCGACGGCCTCGTCCGAATCTACCATCGCCCCATCCATGTTTCCGAGGTCTTGCTTGAATTCCCCAAGCATTCCGTCTGCCGATCCGATTCCTTTTACATCGGCCAGAAAATCCCTCCTCTCGCCGAACACGACCAGCTCCAGCTCGGCCACAAATACTTCCTTCTCCCCAACCCATTCTTCCACTCTGCTCTCTCTTTCGTCACCATCGCTTCTTTCTTTTCCTCTTCCAACGCCAACAACACCACCACCACCACCAACGTCAGATTCATCAACAACGCCGCCGCCTGCCAAAACCCTTTCGACATCCAGCGAACTCCCTCCGGCTGCCTCCGAATCCGCGTCTCCGATGAGTTCATTTCTCAGTTGCTCGAACAGGGCAACCCCAAAATCCAACAACAACAACAACAACAGCAATCCTCATCGTTACCGCTCGGAAAAATCTGCACGACGCCCCAATTGGCCAAAGACTACGCCCAGCTCGTCAGAACGCGCCGATGGAAGCCCAAGCTCGAGACCGTTCCAGAGATCACTAAACCAAAAGGGATTTCGCCGTTTGGGTTGAAGAAGAGGAAGCCCTTTCATTTCCAATTTCAATTGAATCCGCCGCCGTCGCCGCCGCTCAAGTCGGCTGCATCGGCTTGGACCAAATCGGCCTACCGACTTCACCATCTCCGTTCGATTCAGTTTGCTTATAAGCCTAAGATTCGCATCAGATCCAAGGCTTAA

Protein sequence

MKLLTVQKNMVSWSCFHLVKNGIPDSPIANIKLIHSDGLVRIYHRPIHVSEVLLEFPKHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNTTTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQGNPKIQQQQQQQQSSSLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLNPPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSKA
Homology
BLAST of Lag0022052 vs. NCBI nr
Match: XP_038905326.1 (uncharacterized protein LOC120091392 [Benincasa hispida])

HSP 1 Score: 366.7 bits (940), Expect = 1.8e-97
Identity = 198/277 (71.48%), Postives = 221/277 (79.78%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVKNGIPDSPIANIKLIHSDGLVRIYHRPIHVSEVLLEFPKHS 60
           +K+L+VQ NMVSWSCFHL+KN IP+   ANIKLI+SDG+VRIYH+PI+VSEVLLEFPKH 
Sbjct: 3   IKVLSVQ-NMVSWSCFHLMKNAIPEPAAANIKLINSDGVVRIYHQPIYVSEVLLEFPKHL 62

Query: 61  VCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNTTTT 120
           VCRSDSFYIGQKIPPL++ D LQLGHKYFLLP PFFHS LSFVTIASFFS+S +N+    
Sbjct: 63  VCRSDSFYIGQKIPPLSDQDLLQLGHKYFLLPKPFFHSVLSFVTIASFFSASASNSNNKN 122

Query: 121 TNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQSSSLP 180
              RF+NNAAAC  PFD+QRTPSGCLRIRVSDEFISQLLEQG NPK      QQ +SSLP
Sbjct: 123 ---RFVNNAAACHRPFDLQRTPSGCLRIRVSDEFISQLLEQGNNPK--PLSHQQSASSLP 182

Query: 181 LGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLNPPP 240
           LGKICTTPQLAKDY QLVRTR+WKPKLET+ E TK K IS FGLKK  PFH         
Sbjct: 183 LGKICTTPQLAKDYTQLVRTRQWKPKLETIIE-TKKKRISTFGLKKANPFH--------- 242

Query: 241 SPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSK 277
           SPPL        +S+Y LHHLRSI  AYKPKIRI+S+
Sbjct: 243 SPPL--------RSSYPLHHLRSIPLAYKPKIRIKSR 255

BLAST of Lag0022052 vs. NCBI nr
Match: XP_008465162.1 (PREDICTED: uncharacterized protein LOC103502830 [Cucumis melo] >TYK10680.1 DUF4228 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 355.9 bits (912), Expect = 3.1e-94
Identity = 198/281 (70.46%), Postives = 219/281 (77.94%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVKNGIPDSPIA---NIKLIHSDGLVRIYHRPIHVSEVLLEFP 60
           +K+L+VQ NMVSWSCFHL+KN +P  P +   NIKLI SDG+VRIYHRPI+VSEVLLEFP
Sbjct: 3   IKVLSVQ-NMVSWSCFHLMKNALPPDPASDNTNIKLILSDGVVRIYHRPIYVSEVLLEFP 62

Query: 61  KHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNT 120
           KHSVCRSDSFYIGQKIPPL++ D LQLGHKYFLLP PFFHS LSFVTIASFFSSSN+NN 
Sbjct: 63  KHSVCRSDSFYIGQKIPPLSDQDLLQLGHKYFLLPTPFFHSVLSFVTIASFFSSSNSNNK 122

Query: 121 TTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQSS 180
                 RFINNAAAC  PFD+QRTPSGCLRIRVSDEFISQLLEQG NPK       QQ+ 
Sbjct: 123 N-----RFINNAAACHCPFDLQRTPSGCLRIRVSDEFISQLLEQGNNPK---PLPPQQTP 182

Query: 181 SLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLN 240
           SLPLGKICTTPQLAKDY QLVRTRRWKPKLET+ E T+ K +S FGLKK  PF       
Sbjct: 183 SLPLGKICTTPQLAKDYTQLVRTRRWKPKLETIKE-TQKKRVSSFGLKKAIPFQ------ 242

Query: 241 PPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSKA 278
              S P+        +SAY L HLRSI FAYK KIRI+S++
Sbjct: 243 ---STPI--------RSAYPLPHLRSIHFAYKHKIRIKSRS 256

BLAST of Lag0022052 vs. NCBI nr
Match: XP_011652332.1 (uncharacterized protein LOC105435015 [Cucumis sativus])

HSP 1 Score: 351.3 bits (900), Expect = 7.7e-93
Identity = 196/282 (69.50%), Postives = 216/282 (76.60%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVKNGIPDSP----IANIKLIHSDGLVRIYHRPIHVSEVLLEF 60
           +K+L+VQ NMVSWSCFHL+KN IP  P      NIKLIHSDG+VRIYHRPI+VSEVLLEF
Sbjct: 3   IKVLSVQ-NMVSWSCFHLMKNAIPPPPPSSDSTNIKLIHSDGVVRIYHRPIYVSEVLLEF 62

Query: 61  PKHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANN 120
           PKH VCRSDSFYIGQKIPPL++ D LQLGHKYFLLP  FFHS LSFVTIASFFSSSN NN
Sbjct: 63  PKHLVCRSDSFYIGQKIPPLSDQDLLQLGHKYFLLPTSFFHSVLSFVTIASFFSSSNNNN 122

Query: 121 TTTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQS 180
                  RFINNAAA   PFD+QRTPSGCL+IRVSD+FISQLLEQG NPK       QQS
Sbjct: 123 KN-----RFINNAAASHCPFDLQRTPSGCLKIRVSDDFISQLLEQGNNPK---PLPPQQS 182

Query: 181 SSLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQL 240
            SLPLGKICTTPQLAKDY QLVRTR+WKPKLET+ E T+ K IS FGLKK  PF      
Sbjct: 183 PSLPLGKICTTPQLAKDYTQLVRTRKWKPKLETIKE-TQKKRISSFGLKKANPF------ 242

Query: 241 NPPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSKA 278
              PS P+        +S Y LHHLRSI FAYK KIRI++++
Sbjct: 243 ---PSTPI--------RSPYPLHHLRSIHFAYKHKIRIKARS 257

BLAST of Lag0022052 vs. NCBI nr
Match: KAG6581636.1 (hypothetical protein SDJN03_21638, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018132.1 hypothetical protein SDJN02_20000, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336.3 bits (861), Expect = 2.6e-88
Identity = 188/279 (67.38%), Postives = 205/279 (73.48%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVK---NGIPDSPIANIKLIHSDGLVRIYHRPIHVSEVLLEFP 60
           MK L+VQ NMVSWSCFHL K     IPD P ANIKLI S G+VR+YHRPI VS++LLEFP
Sbjct: 3   MKALSVQ-NMVSWSCFHLAKTTTTDIPD-PSANIKLILSHGVVRVYHRPISVSQILLEFP 62

Query: 61  KHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNT 120
           KH VCRSD+FYIGQKIP L+EHD LQLGHKYFLLP PFFHS LSFV+IASFFS  N  N 
Sbjct: 63  KHLVCRSDAFYIGQKIPHLSEHDFLQLGHKYFLLPKPFFHSVLSFVSIASFFSPPNKPN- 122

Query: 121 TTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQGNPKIQQQQQQQQSSS 180
                 RF  NAAAC +PFD+QRTPSGCLRIRVSDEFISQLLEQGNPK         S  
Sbjct: 123 ------RFHTNAAACLHPFDLQRTPSGCLRIRVSDEFISQLLEQGNPK------PLPSPP 182

Query: 181 LPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLNP 240
           LP GKICTTPQLAK+Y QLVRTRRWKPKLET+ E  K  GISPFGLKK  PF        
Sbjct: 183 LPQGKICTTPQLAKEYTQLVRTRRWKPKLETIEETKKKGGISPFGLKKGNPF-------- 242

Query: 241 PPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSK 277
            PSPP         +S+Y LHHLR+I   YKPKIRI+S+
Sbjct: 243 -PSPP--------PRSSYSLHHLRAIH--YKPKIRIKSR 247

BLAST of Lag0022052 vs. NCBI nr
Match: KAG6581637.1 (hypothetical protein SDJN03_21639, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.5 bits (859), Expect = 4.4e-88
Identity = 188/278 (67.63%), Postives = 204/278 (73.38%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVK---NGIPDSPIANIKLIHSDGLVRIYHRPIHVSEVLLEFP 60
           MK L+VQ NMVSWSCFHL K     IPD P ANIKLI S G+VR+YHRPI VS++LLEFP
Sbjct: 3   MKALSVQ-NMVSWSCFHLAKTTTTDIPD-PSANIKLILSHGVVRVYHRPISVSQILLEFP 62

Query: 61  KHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNT 120
           KH VCRSD+FYIGQKIP L+EHD LQLGHKYFLLP PFFHS LSFV+IASFFS  N  N 
Sbjct: 63  KHLVCRSDAFYIGQKIPHLSEHDFLQLGHKYFLLPKPFFHSVLSFVSIASFFSPPNKPN- 122

Query: 121 TTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQGNPKIQQQQQQQQSSS 180
                 RF  NAAAC +PFD+QRTPSGCLRIRVSDEFISQLLEQGNPK         S  
Sbjct: 123 ------RFHTNAAACLHPFDLQRTPSGCLRIRVSDEFISQLLEQGNPK------PLPSPP 182

Query: 181 LPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLNP 240
           LP GKICTTPQLAK+Y QLVRTRRWKPKLET+ E  K  GISPFGLKK  PF        
Sbjct: 183 LPQGKICTTPQLAKEYTQLVRTRRWKPKLETIEETKKKGGISPFGLKKGNPF-------- 242

Query: 241 PPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRS 276
            PSPP         +S+Y LHHLR+I   YKPKIRI+S
Sbjct: 243 -PSPP--------PRSSYSLHHLRAIH--YKPKIRIKS 246

BLAST of Lag0022052 vs. ExPASy TrEMBL
Match: A0A5D3CH36 (DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00450 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.5e-94
Identity = 198/281 (70.46%), Postives = 219/281 (77.94%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVKNGIPDSPIA---NIKLIHSDGLVRIYHRPIHVSEVLLEFP 60
           +K+L+VQ NMVSWSCFHL+KN +P  P +   NIKLI SDG+VRIYHRPI+VSEVLLEFP
Sbjct: 3   IKVLSVQ-NMVSWSCFHLMKNALPPDPASDNTNIKLILSDGVVRIYHRPIYVSEVLLEFP 62

Query: 61  KHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNT 120
           KHSVCRSDSFYIGQKIPPL++ D LQLGHKYFLLP PFFHS LSFVTIASFFSSSN+NN 
Sbjct: 63  KHSVCRSDSFYIGQKIPPLSDQDLLQLGHKYFLLPTPFFHSVLSFVTIASFFSSSNSNNK 122

Query: 121 TTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQSS 180
                 RFINNAAAC  PFD+QRTPSGCLRIRVSDEFISQLLEQG NPK       QQ+ 
Sbjct: 123 N-----RFINNAAACHCPFDLQRTPSGCLRIRVSDEFISQLLEQGNNPK---PLPPQQTP 182

Query: 181 SLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLN 240
           SLPLGKICTTPQLAKDY QLVRTRRWKPKLET+ E T+ K +S FGLKK  PF       
Sbjct: 183 SLPLGKICTTPQLAKDYTQLVRTRRWKPKLETIKE-TQKKRVSSFGLKKAIPFQ------ 242

Query: 241 PPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSKA 278
              S P+        +SAY L HLRSI FAYK KIRI+S++
Sbjct: 243 ---STPI--------RSAYPLPHLRSIHFAYKHKIRIKSRS 256

BLAST of Lag0022052 vs. ExPASy TrEMBL
Match: A0A1S3CNA0 (uncharacterized protein LOC103502830 OS=Cucumis melo OX=3656 GN=LOC103502830 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.5e-94
Identity = 198/281 (70.46%), Postives = 219/281 (77.94%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVKNGIPDSPIA---NIKLIHSDGLVRIYHRPIHVSEVLLEFP 60
           +K+L+VQ NMVSWSCFHL+KN +P  P +   NIKLI SDG+VRIYHRPI+VSEVLLEFP
Sbjct: 3   IKVLSVQ-NMVSWSCFHLMKNALPPDPASDNTNIKLILSDGVVRIYHRPIYVSEVLLEFP 62

Query: 61  KHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNT 120
           KHSVCRSDSFYIGQKIPPL++ D LQLGHKYFLLP PFFHS LSFVTIASFFSSSN+NN 
Sbjct: 63  KHSVCRSDSFYIGQKIPPLSDQDLLQLGHKYFLLPTPFFHSVLSFVTIASFFSSSNSNNK 122

Query: 121 TTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQSS 180
                 RFINNAAAC  PFD+QRTPSGCLRIRVSDEFISQLLEQG NPK       QQ+ 
Sbjct: 123 N-----RFINNAAACHCPFDLQRTPSGCLRIRVSDEFISQLLEQGNNPK---PLPPQQTP 182

Query: 181 SLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLN 240
           SLPLGKICTTPQLAKDY QLVRTRRWKPKLET+ E T+ K +S FGLKK  PF       
Sbjct: 183 SLPLGKICTTPQLAKDYTQLVRTRRWKPKLETIKE-TQKKRVSSFGLKKAIPFQ------ 242

Query: 241 PPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSKA 278
              S P+        +SAY L HLRSI FAYK KIRI+S++
Sbjct: 243 ---STPI--------RSAYPLPHLRSIHFAYKHKIRIKSRS 256

BLAST of Lag0022052 vs. ExPASy TrEMBL
Match: A0A0A0LCN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G846010 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.7e-93
Identity = 196/282 (69.50%), Postives = 216/282 (76.60%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVKNGIPDSP----IANIKLIHSDGLVRIYHRPIHVSEVLLEF 60
           +K+L+VQ NMVSWSCFHL+KN IP  P      NIKLIHSDG+VRIYHRPI+VSEVLLEF
Sbjct: 3   IKVLSVQ-NMVSWSCFHLMKNAIPPPPPSSDSTNIKLIHSDGVVRIYHRPIYVSEVLLEF 62

Query: 61  PKHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANN 120
           PKH VCRSDSFYIGQKIPPL++ D LQLGHKYFLLP  FFHS LSFVTIASFFSSSN NN
Sbjct: 63  PKHLVCRSDSFYIGQKIPPLSDQDLLQLGHKYFLLPTSFFHSVLSFVTIASFFSSSNNNN 122

Query: 121 TTTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQS 180
                  RFINNAAA   PFD+QRTPSGCL+IRVSD+FISQLLEQG NPK       QQS
Sbjct: 123 KN-----RFINNAAASHCPFDLQRTPSGCLKIRVSDDFISQLLEQGNNPK---PLPPQQS 182

Query: 181 SSLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQL 240
            SLPLGKICTTPQLAKDY QLVRTR+WKPKLET+ E T+ K IS FGLKK  PF      
Sbjct: 183 PSLPLGKICTTPQLAKDYTQLVRTRKWKPKLETIKE-TQKKRISSFGLKKANPF------ 242

Query: 241 NPPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSKA 278
              PS P+        +S Y LHHLRSI FAYK KIRI++++
Sbjct: 243 ---PSTPI--------RSPYPLHHLRSIHFAYKHKIRIKARS 257

BLAST of Lag0022052 vs. ExPASy TrEMBL
Match: A0A6J1F4J4 (uncharacterized protein LOC111442099 OS=Cucurbita moschata OX=3662 GN=LOC111442099 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.8e-88
Identity = 188/280 (67.14%), Postives = 204/280 (72.86%), Query Frame = 0

Query: 1   MKLLTVQKNMVSWSCFHLVK----NGIPDSPIANIKLIHSDGLVRIYHRPIHVSEVLLEF 60
           MK L+VQ NMVSWSCFHL K      IPD P ANIKLI S G+VR+YHRPI VSE+LLEF
Sbjct: 3   MKALSVQ-NMVSWSCFHLAKATTTTDIPD-PSANIKLILSHGVVRVYHRPISVSEILLEF 62

Query: 61  PKHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANN 120
           PKH VCRSD+FYIGQKIP L+EHD LQLGHKYFLLP PFFHS LSFV+IASFFS  N  N
Sbjct: 63  PKHLVCRSDAFYIGQKIPHLSEHDFLQLGHKYFLLPKPFFHSVLSFVSIASFFSPPNKPN 122

Query: 121 TTTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQGNPKIQQQQQQQQSS 180
                  RF  NAAAC +PFD+QRTPSGCLRIRVSDEFISQLLEQGNPK         S 
Sbjct: 123 -------RFHTNAAACLHPFDLQRTPSGCLRIRVSDEFISQLLEQGNPK------PLPSP 182

Query: 181 SLPLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLN 240
            LP GKICTTPQLAK+Y QLVR RRWKPKLET+ E  K  GISPFGLKK  PF       
Sbjct: 183 PLPQGKICTTPQLAKEYTQLVRARRWKPKLETIEETKKKGGISPFGLKKGNPF------- 242

Query: 241 PPPSPPLKSAASAWTKSAYRLHHLRSIQFAYKPKIRIRSK 277
             PSPP         +S+Y LHHLR+I   YKPKIRI+S+
Sbjct: 243 --PSPP--------PRSSYSLHHLRAIH--YKPKIRIKSR 248

BLAST of Lag0022052 vs. ExPASy TrEMBL
Match: A0A5A7T3Y8 (DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1593G00170 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 2.0e-86
Identity = 184/263 (69.96%), Postives = 202/263 (76.81%), Query Frame = 0

Query: 19  VKNGIPDSPIA---NIKLIHSDGLVRIYHRPIHVSEVLLEFPKHSVCRSDSFYIGQKIPP 78
           +KN +P  P +   NIKLI SDG+VRIYHRPI+VSEVLLEFPKHSVCRSDSFYIGQKIPP
Sbjct: 1   MKNALPPDPASDNTNIKLILSDGVVRIYHRPIYVSEVLLEFPKHSVCRSDSFYIGQKIPP 60

Query: 79  LAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNTTTTTNVRFINNAAACQNP 138
           L++ D LQLGHKYFLLP PFFHS LSFVTIASFFSSSN+NN       RFINNAAAC  P
Sbjct: 61  LSDQDLLQLGHKYFLLPTPFFHSVLSFVTIASFFSSSNSNNKN-----RFINNAAACHCP 120

Query: 139 FDIQRTPSGCLRIRVSDEFISQLLEQG-NPKIQQQQQQQQSSSLPLGKICTTPQLAKDYA 198
           FD+QRTPSGCLRIRVSDEFISQLLEQG NPK       QQ+ SLPLGKICTTPQLAKDY 
Sbjct: 121 FDLQRTPSGCLRIRVSDEFISQLLEQGNNPK---PLPPQQTPSLPLGKICTTPQLAKDYT 180

Query: 199 QLVRTRRWKPKLETVPEITKPKGISPFGLKKRKPFHFQFQLNPPPSPPLKSAASAWTKSA 258
           QLVRTRRWKPKLET+ E T+ K +S FGLKK  PF          S P+        +SA
Sbjct: 181 QLVRTRRWKPKLETIKE-TQKKRVSSFGLKKAIPFQ---------STPI--------RSA 237

Query: 259 YRLHHLRSIQFAYKPKIRIRSKA 278
           Y L HLRSI FAYK KIRI+S++
Sbjct: 241 YPLPHLRSIHFAYKHKIRIKSRS 237

BLAST of Lag0022052 vs. TAIR 10
Match: AT1G28190.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12340.1); Has 166 Blast hits to 162 proteins in 36 species: Archae - 0; Bacteria - 2; Metazoa - 15; Fungi - 5; Plants - 124; Viruses - 0; Other Eukaryotes - 20 (source: NCBI BLink). )

HSP 1 Score: 161.8 bits (408), Expect = 8.1e-40
Identity = 95/229 (41.48%), Postives = 136/229 (59.39%), Query Frame = 0

Query: 3   LLTVQKNMVSWSCFHLVKNGIPD--SPIANIKLIHSDGLVRIYHRPIHVSEVLLEFPKHS 62
           L+ + K   SW C H      PD  S    IKL+ SDG +++Y RP+ VSE+  +FPKH 
Sbjct: 13  LVKLHKGPSSWLCLH----PRPDRTSNGGRIKLVRSDGSLQVYDRPVVVSELTKDFPKHK 72

Query: 63  VCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIASFFSSSNANNTTTT 122
           +CRSD  YIGQK P L+E + L+LG  YFLLP+ FF + LSF+TIA+  +  N       
Sbjct: 73  ICRSDLLYIGQKTPVLSETETLKLGLNYFLLPSDFFKNDLSFLTIATLKTPQNGGVLVKK 132

Query: 123 TNVRFINNAAACQNPFDIQRTPSG-CLRIRVSDEFISQLLEQGNP-KIQQQQQQQQSSSL 182
           T  +          PF IQ+   G  LRIRVS+EF+S+L+ +G   ++ +++++++    
Sbjct: 133 TQQQ--------PQPFLIQKGEKGERLRIRVSEEFVSELMMEGKKNRVNEEEEEEEGGGE 192

Query: 183 PLGKICTTPQLAKDYAQLVRTRRWKPKLETVPEITKPKGISPFGLKKRK 228
             G++CTT +L KDY QLV  RRWKPKLET+ E    K  +    KK++
Sbjct: 193 GEGRVCTTVKLKKDYVQLVGLRRWKPKLETITETKAMKAATMEKTKKKR 229

BLAST of Lag0022052 vs. TAIR 10
Match: AT5G12340.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G28190.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 92.8 bits (229), Expect = 4.6e-19
Identity = 67/186 (36.02%), Postives = 101/186 (54.30%), Query Frame = 0

Query: 48  HVS-EVLLEFPKHSVCRSDSFYIGQKIPPLAEHDQLQLGHKYFLLPNPFFHSALSFVTIA 107
           HV+ E++ EFP   VC +DSFYIG++IP LA  D L  G  YF+LP   F   +   +  
Sbjct: 39  HVAGEIMFEFPDQIVCHADSFYIGRQIPALAMDDYLIPGQTYFVLPIERFQYKILTTSCL 98

Query: 108 SFFSSSNANNTTTTTNVRFINNAAACQNPFDIQRTPSGCLRIRVSDEFISQLLEQGNPKI 167
           S F+S+  N  + T  + F   A +   PF+  + P+G + I+VS EFI  L+     K 
Sbjct: 99  SIFNSNLENVRSNTPPLNF--TAPSSPPPFEYSQGPNGKILIKVSPEFIISLI----CKN 158

Query: 168 QQQQQQQQSSSLPLGKICTTPQLAKDYAQLVRTRR--WKPKLETVPEITKPKGISP---F 227
           +  ++++ +S      IC +P+L K Y QLV TR   W P L+T+ E      +SP   F
Sbjct: 159 RNIREEEATSCGENSAICNSPELKKQYDQLVGTREHMWSPNLQTISE--HKIRVSPLRFF 216

BLAST of Lag0022052 vs. TAIR 10
Match: AT3G03280.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G17350.1); Has 137 Blast hits to 137 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 137; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-05
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0

Query: 21 NGIPDSPIANIKLIHSDGLVRIYHRPIHVSEVLLEFPKHSVCRSDSFYIGQKIPPLAEHD 80
          N    SP+A  K+I  DG VR  H P   +E+++E P + +  + S  +G+K  PLA  D
Sbjct: 10 NKTSSSPLA--KVILPDGGVRDIHVPTKAAELMMEMPSYFLVDTKSVKVGRKFIPLAADD 69

Query: 81 QLQLG--HKYFLLP 93
           L LG  H Y   P
Sbjct: 70 DLDLGGCHVYVAFP 81

BLAST of Lag0022052 vs. TAIR 10
Match: AT5G17350.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 8 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G03280.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 44.3 bits (103), Expect = 1.9e-04
Identity = 28/80 (35.00%), Postives = 40/80 (50.00%), Query Frame = 0

Query: 32  KLIHSDGLVRIYHRPIHVSEVLLEFPKHSVCRSDSFYIGQKIPPLAEHDQLQLG--HKYF 91
           K+I  DG VR  H P+  +E+++E P   +  + S  IG+K  PLA  D LQ+   H Y 
Sbjct: 21  KVILPDGGVRNIHAPMKAAELMMEIPSFFLVDAKSLKIGRKFCPLAADDDLQIKGCHVYV 80

Query: 92  LLPNPFFHSALSFVTIASFF 110
             P     SA +   +A  F
Sbjct: 81  AFPMTRATSAANASDLARLF 100

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905326.11.8e-9771.48uncharacterized protein LOC120091392 [Benincasa hispida][more]
XP_008465162.13.1e-9470.46PREDICTED: uncharacterized protein LOC103502830 [Cucumis melo] >TYK10680.1 DUF42... [more]
XP_011652332.17.7e-9369.50uncharacterized protein LOC105435015 [Cucumis sativus][more]
KAG6581636.12.6e-8867.38hypothetical protein SDJN03_21638, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6581637.14.4e-8867.63hypothetical protein SDJN03_21639, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3CH361.5e-9470.46DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CNA01.5e-9470.46uncharacterized protein LOC103502830 OS=Cucumis melo OX=3656 GN=LOC103502830 PE=... [more]
A0A0A0LCN93.7e-9369.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G846010 PE=4 SV=1[more]
A0A6J1F4J42.8e-8867.14uncharacterized protein LOC111442099 OS=Cucurbita moschata OX=3662 GN=LOC1114420... [more]
A0A5A7T3Y82.0e-8669.96DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
Match NameE-valueIdentityDescription
AT1G28190.18.1e-4041.48unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12340.14.6e-1936.02unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G03280.11.3e-0537.84unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT5G17350.11.9e-0435.00unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025322Protein of unknown function DUF4228, plantPFAMPF14009DUF4228coord: 29..211
e-value: 3.7E-30
score: 105.7
NoneNo IPR availablePANTHERPTHR33052DUF4228 DOMAIN PROTEIN-RELATEDcoord: 7..218
NoneNo IPR availablePANTHERPTHR33052:SF91F3H9.15 PROTEINcoord: 7..218

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0022052.1Lag0022052.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane