Lag0022012 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0022012 vs. NCBI nr
Match: EEC84982.1 (hypothetical protein OsI_32248 [Oryza sativa Indica Group] >EEE62926.1 hypothetical protein OsJ_17731 [Oryza sativa Japonica Group])

HSP 1 Score: 258.5 bits (659), Expect = 7.8e-65
Identity = 128/288 (44.44%), Postives = 184/288 (63.89%), Query Frame = 0

Query: 52  NETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISHNQSAFIPGR 111
           N+T++ LIPK + P R+ + +PISLC V YKL SKVL NRLK +L ++IS NQSAF+P R
Sbjct: 160 NDTVVTLIPKVQSPERLKDLRPISLCTVVYKLASKVLSNRLKLILPDIISPNQSAFVPQR 219

Query: 112 CVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVMLKVSYAPGWVK 171
            + DN +L YE  H ++ +  GR  +A+LKLDMSK YDRVEW F+EK+M+++ +A GWVK
Sbjct: 220 LITDNVLLAYEMTHFMQTKRTGREGYAALKLDMSKAYDRVEWSFLEKMMVRLGFAEGWVK 279

Query: 172 LVSLCISSVKFSFNVNGVRCGDVVPS--------------------LSGMLRGAEEAKSI 231
           L+  C+S+V +   VNG     ++PS                     S +L  AEE   +
Sbjct: 280 LIMRCVSTVTYRIKVNGDLTDQIIPSRGLRQGDPISPYLFLICAEGFSSLLYAAEERGDL 339

Query: 232 KGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNLDKSVISFS 291
            G+K+ Q  P++SH+ FA D LLLF+  ER A+ ++++LN YE  SGQ VN DKS I FS
Sbjct: 340 SGVKVCQQAPSVSHLLFADDSLLLFKVNERSAQCLQNVLNLYESCSGQIVNKDKSSIMFS 399

Query: 292 LSTNARDRTQVGQILGVQVVACHRQYLGLPSFMPRNKMSSLNFIKDRV 320
            +T+  DR  V +IL +   A + +YLGLP +M R++  +  ++K+RV
Sbjct: 400 KNTSQADRKMVMEILDISTEARNEKYLGLPVYMGRSRAKTFAYLKERV 447

BLAST of Lag0022012 vs. NCBI nr
Match: XP_030923826.1 (uncharacterized protein LOC115950728 [Quercus lobata])

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-63
Identity = 135/355 (38.03%), Postives = 206/355 (58.03%), Query Frame = 0

Query: 2   GGKNTRWFHTHASSRRRKNEVRDLEDGDGVWQQDPGRVLRLIE----------------- 61
           G KNT++FH+ AS+RRR+N ++ +   D  W +D   + ++                   
Sbjct: 279 GDKNTKFFHSKASNRRRRNLIQGIRTQDNRWVEDISDIAQVATSYFEDMFKAAVLDFLNF 338

Query: 62  GVSPALLNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISHNQ 121
           G     +N T IVLIPK + P ++S+Y+PISLCNV YK++SKVL NRLK +L ++IS  Q
Sbjct: 339 GNMDPGVNYTHIVLIPKIKSPEKMSDYRPISLCNVIYKIISKVLANRLKQILPQIISPTQ 398

Query: 122 SAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVMLKVS 181
           SAFIPGR + DN ++ YECLH +  R +G+    +LKLD+SK YDRVEW F++ +M K+ 
Sbjct: 399 SAFIPGRLITDNIMVAYECLHAMHCRKKGKKGSLALKLDISKAYDRVEWAFLKGIMEKMG 458

Query: 182 YAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPS--------------------LSGMLRG 241
           +   W+  V  C+S+  FS  +NG   G+++PS                     + +L  
Sbjct: 459 FPEIWIDRVMSCVSTPSFSVCINGKPFGNILPSRGIRQGDPLSPYLFLLCAEGFTSLLAK 518

Query: 242 AEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNLD 301
           AE    I G+ I +  P IS++ FA D LL  +A + + +V+ ++L+ Y   SGQ +N +
Sbjct: 519 AELEGQIHGMSICRRAPCISNLLFADDSLLFCKATQGEVQVLMEMLDLYASASGQCINFE 578

Query: 302 KSVISFSLSTNARDRTQVGQILGVQVVACHRQYLGLPSFMPRNKMSSLNFIKDRV 320
           KS I F  +T A  + ++  +LGV+ VA    YLGLP+ + R+K  + +F+KDRV
Sbjct: 579 KSSIFFGSNTEASHKERIINLLGVKEVARFESYLGLPTLIGRSKYQTFSFLKDRV 633

BLAST of Lag0022012 vs. NCBI nr
Match: XP_030940187.1 (uncharacterized protein LOC115965136 [Quercus lobata])

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-63
Identity = 143/425 (33.65%), Postives = 213/425 (50.12%), Query Frame = 0

Query: 2   GGKNTRWFHTHASSRRRKNEVRDLEDGDGVWQQDPGR----------------------- 61
           G +NT++FH  A+ RR +N +  L D +G+WQ+DPG                        
Sbjct: 250 GDRNTKFFHATANQRRWRNSITGLVDSNGIWQEDPGAMEGITLDYFETIFKSNNPSSFDA 309

Query: 62  ------------------------------------------------------------ 121
                                                                       
Sbjct: 310 CVRTITPKVTPEMNAALCAEFCASEVWNALHQMHPTKAPGPDGMSLIFFQKYWDVVGVNV 369

Query: 122 ---VLRLIE-GVSPALLNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKG 181
              VL ++  GV P  +NET I LIPK + PR++SEY+PISLCNV YK+VSK+L NRLK 
Sbjct: 370 IDCVLEILNTGVMPCGVNETYICLIPKTKTPRKISEYRPISLCNVIYKIVSKILANRLKR 429

Query: 182 VLNEVISHNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWV 241
           +L EVI  +QSAF+PGR + DN ++ +E +H + GR +GR    +LKLDMSK YDRVEW 
Sbjct: 430 ILTEVIDESQSAFVPGRLITDNVLVAFETMHCIDGRKKGREALMALKLDMSKAYDRVEWQ 489

Query: 242 FMEKVMLKVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPS----------------- 301
           F+E +M K+ +   W+ L+ +CIS+V +S  +NG   G ++PS                 
Sbjct: 490 FLEMIMRKLGFHKKWISLMMMCISTVSYSVLINGEAKGKIIPSRGLRQGDPISPYLFLLC 549

Query: 302 ---LSGMLRGAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYE 320
              L   L+  E   +I+G+ + +  P ISH+FFA D ++  RA   + + V ++L  YE
Sbjct: 550 TEGLFARLKKEEIEGNIRGVSVRRGAPQISHLFFADDSIIFCRATVEEGKRVLNVLEEYE 609

BLAST of Lag0022012 vs. NCBI nr
Match: XP_030505314.1 (uncharacterized protein LOC115720302 [Cannabis sativa])

HSP 1 Score: 252.7 bits (644), Expect = 4.3e-63
Identity = 130/292 (44.52%), Postives = 182/292 (62.33%), Query Frame = 0

Query: 48  PALLNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISHNQSAF 107
           P+ +N+T+I LIPK + P  V++Y+PISLCNV YKL+SK +V R+K VL+ VIS  QSAF
Sbjct: 494 PSCINKTLITLIPKVKHPTSVTQYRPISLCNVIYKLISKAIVLRMKPVLHLVISEFQSAF 553

Query: 108 IPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVMLKVSYAP 167
           I  R ++DN ++ YE LH LR ++RGR  +A+LKLDMSK +DRVEW F+E+V+LK+ +  
Sbjct: 554 IFDRLIIDNVLVAYELLHCLRNKTRGRVSYAALKLDMSKAFDRVEWHFLERVLLKMGFGH 613

Query: 168 GWVKLVSLCISSVKFSFNVNGVRCGDVVP--------------------SLSGMLRGAEE 227
           G V L+  CISS  FSF +NG   G + P                    +LS +L+  E+
Sbjct: 614 GLVGLIMRCISSTSFSFLINGHVTGQLQPQRGIPQGDPLSPYLFLVCSEALSRLLQLHEQ 673

Query: 228 AKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNLDKSV 287
              + GL + +  P +SH+ FA D LL  R   R   VV+  L+ Y   SGQ +N DKSV
Sbjct: 674 QGMLTGLGVTRQAPKVSHLLFANDSLLFCRTDARSLAVVKHTLDVYSNASGQLINYDKSV 733

Query: 288 ISFSLSTNARDRTQVGQILGVQVVACHRQYLGLPSFMPRNKMSSLNFIKDRV 320
           +SFS +T+  D+     +LG+ + +CH QYLGLPS+   NK    N IK+R+
Sbjct: 734 MSFSPNTSTADQNAFSLLLGMPIQSCHEQYLGLPSYTGHNKSEEFNEIKNRI 785

BLAST of Lag0022012 vs. NCBI nr
Match: XP_030969964.1 (uncharacterized protein LOC115990257 [Quercus lobata])

HSP 1 Score: 252.7 bits (644), Expect = 4.3e-63
Identity = 136/358 (37.99%), Postives = 206/358 (57.54%), Query Frame = 0

Query: 13  ASSRRRKNEVRDLEDGDGVWQQDPGRVLRLI----------------------------- 72
           A+ R + N +  LED  GVW +D  ++ RL                              
Sbjct: 181 ANQRNKHNLIVGLEDEFGVWTEDEDQMGRLATEEVLKALHHMAPLIAPGPDDVTKVVLTA 240

Query: 73  --EGVSPALLNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVIS 132
                 PA +N T I L PK + P++VS+++PISLCNV YKL++KVLVNRLK +L  V+S
Sbjct: 241 LNSSTIPASINFTFIALTPKIKNPKKVSDFRPISLCNVVYKLIAKVLVNRLKLILPYVVS 300

Query: 133 HNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVML 192
            +QSAF+ GR + DN ++ +E LH L+ +++G+  + +LKLD+SK YDRVEW F+E+ ML
Sbjct: 301 DSQSAFLSGRLITDNVLVAFETLHFLKRKTQGKDGYMALKLDISKAYDRVEWDFLERAML 360

Query: 193 KVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPS--------------------LSGM 252
            + +A  +V  +  CI S+ +S  +NGV    + PS                    L G+
Sbjct: 361 HLGFAGSFVATIMSCIKSISYSVLLNGVLGRTIKPSRGLRQGDPLSPYLFLLCAMGLQGL 420

Query: 253 LRGAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTV 312
           L  AE    I+G+ I + GP +SH+FFA D +L  RAKE + +V+ D+L+ YERGSGQ +
Sbjct: 421 LHKAESEGVIRGVSICKNGPRVSHLFFADDSVLFCRAKESECQVILDLLSVYERGSGQKI 480

Query: 313 NLDKSVISFSLSTNARDRTQVGQILGVQVVACHRQYLGLPSFMPRNKMSSLNFIKDRV 320
           N DK+ I F+ +T+   + Q+  +LGV  +  +++YLGLP+F+ R K     +IK+R+
Sbjct: 481 NRDKTNIFFNSNTHHDVQVQIQHLLGVPAIRQYKRYLGLPAFVGRTKRQGFAYIKERI 538

BLAST of Lag0022012 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.4e-18
Identity = 73/277 (26.35%), Postives = 122/277 (44.04%), Query Frame = 0

Query: 44  EGVSPALLNETMIVLIPKE-RRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISH 103
           EG+ P    E  I LIPK  + P R   Y+PISL N+  K+++K+L NR++  + ++I H
Sbjct: 501 EGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHH 560

Query: 104 NQSAFIPGRCVVDNAILGYECL-HVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVML 163
           +Q  FIPG     N       + H+ + +++       L +D  K +D ++  FM + + 
Sbjct: 561 DQVGFIPGSQGWFNIRKSINVIQHINKLKNKDH---MILSIDAEKAFDNIQHPFMIRTLK 620

Query: 164 KVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPSLSGMLRGA---------------- 223
           K+     ++KL+    S    +  +NGV+     P  SG  +G                 
Sbjct: 621 KIGIEGTFLKLIEAIYSKPTANIILNGVKLKS-FPLRSGTRQGCPLSPLLFNIVMEVLAI 680

Query: 224 --EEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNL 283
              E K+IKG+ I      I    FA D ++           + +++  Y   SG  +N 
Sbjct: 681 AIREEKAIKGIHIG--SEEIKLSLFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINT 740

Query: 284 DKSVISFSLSTNARDRTQVGQILGVQVVACHRQYLGL 301
            KSV     + N  ++T V   +   VV    +YLG+
Sbjct: 741 HKSVAFIYTNNNQAEKT-VKDSIPFTVVPKKMKYLGV 770

BLAST of Lag0022012 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 5.3e-16
Identity = 64/277 (23.10%), Postives = 127/277 (45.85%), Query Frame = 0

Query: 43  IEGVSPALLNETMIVLIPK-ERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVIS 102
           +EG  P    E  I LIPK ++ P ++  ++PISL N+  K+++K+L NR++  +  +I 
Sbjct: 508 VEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIH 567

Query: 103 HNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVML 162
            +Q  FIPG     N       +H +  + + +     + LD  K +D+++  FM KV+ 
Sbjct: 568 PDQVGFIPGMQGWFNIRKSINVIHYI-NKLKDKNHMI-ISLDAEKAFDKIQHPFMIKVLE 627

Query: 163 KVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPSLSG------------------MLR 222
           +      ++ ++    S    +  VNG +  + +P  SG                  + R
Sbjct: 628 RSGIQGPYLNMIKAIYSKPVANIKVNGEKL-EAIPLKSGTRQGCPLSPYLFNIVLEVLAR 687

Query: 223 GAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNL 282
              + K IKG++I +    IS    A D ++     +     + +++N +    G  +N 
Sbjct: 688 AIRQQKEIKGIQIGKEEVKIS--LLADDMIVYISDPKNSTRELLNLINSFGEVVGYKINS 747

Query: 283 DKSVISFSLSTNARDRTQVGQILGVQVVACHRQYLGL 301
           +KS ++F  + N +   ++ +     +V  + +YLG+
Sbjct: 748 NKS-MAFLYTKNKQAEKEIRETTPFSIVTNNIKYLGV 778

BLAST of Lag0022012 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 9.1e-16
Identity = 65/277 (23.47%), Postives = 129/277 (46.57%), Query Frame = 0

Query: 44  EGVSPALLNETMIVLIPKERRPRRVSE-YQPISLCNVSYKLVSKVLVNRLKGVLNEVISH 103
           EG+ P    E  I+LIPK  R     E ++PISL N+  K+++K+L NR++  + ++I H
Sbjct: 502 EGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHH 561

Query: 104 NQSAFIPGRCVVDNAILGYECL-HVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVML 163
           +Q  FIPG     N       + H+ R + +       + +D  K +D+++  FM K + 
Sbjct: 562 DQVGFIPGMQGWFNIRKSINVIQHINRAKDKNH---VIISIDAEKAFDKIQQPFMLKTLN 621

Query: 164 KVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPSLSG------------------MLR 223
           K+     ++K++         +  +NG +  +  P  +G                  + R
Sbjct: 622 KLGIDGMYLKIIRAIYDKPTANIILNGQKL-EAFPLKTGTRQGCPLSPLLFNIVLEVLAR 681

Query: 224 GAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNL 283
              + K IKG+++ +    +S   FA D ++        A+ +  +++++ + SG  +N+
Sbjct: 682 AIRQEKEIKGIQLGKEEVKLS--LFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINV 741

Query: 284 DKSVISFSLSTNARDRTQVGQILGVQVVACHRQYLGL 301
            KS  +F  + N +  +Q+   L   + +   +YLG+
Sbjct: 742 QKSQ-AFLYNNNRQTESQIMGELPFTIASKRIKYLGI 771

BLAST of Lag0022012 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.0e-14
Identity = 69/242 (28.51%), Postives = 107/242 (44.21%), Query Frame = 0

Query: 44  EGVSPALLNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISHN 103
           +G  P      ++ L+PK+   R +  ++P+SL +  YK+V+K +  RLK VL EVI  +
Sbjct: 498 KGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPD 557

Query: 104 QSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVMLKV 163
           QS  +PGR + DN  L  + LH  R   R     A L LD  K +DRV+  ++   +   
Sbjct: 558 QSYTVPGRTIFDNVFLIRDLLHFAR---RTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAY 617

Query: 164 SYAPGWVKLVSLCISSVKFSFNVN-----------GVRCGDVVPSLSGMLRGAE------ 223
           S+ P +V  +    +S +    +N           GVR G     LSG L          
Sbjct: 618 SFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQG---CPLSGQLYSLAIEPFLC 677

Query: 224 -EAKSIKGLKIAQYGPAISHMFFAY-DRLLLFRAKERDAEVVRDILNHYERGSGQTVNLD 267
              K + GL + +  P +  +  AY D ++L      D E  ++    Y   S   +N  
Sbjct: 678 LLRKRLTGLVLKE--PDMRVVLSAYADDVILVAQDLVDLERAQECQEVYAAASSARINWS 731

BLAST of Lag0022012 vs. ExPASy TrEMBL
Match: B8BE31 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_32248 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 3.8e-65
Identity = 128/288 (44.44%), Postives = 184/288 (63.89%), Query Frame = 0

Query: 52  NETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISHNQSAFIPGR 111
           N+T++ LIPK + P R+ + +PISLC V YKL SKVL NRLK +L ++IS NQSAF+P R
Sbjct: 160 NDTVVTLIPKVQSPERLKDLRPISLCTVVYKLASKVLSNRLKLILPDIISPNQSAFVPQR 219

Query: 112 CVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVMLKVSYAPGWVK 171
            + DN +L YE  H ++ +  GR  +A+LKLDMSK YDRVEW F+EK+M+++ +A GWVK
Sbjct: 220 LITDNVLLAYEMTHFMQTKRTGREGYAALKLDMSKAYDRVEWSFLEKMMVRLGFAEGWVK 279

Query: 172 LVSLCISSVKFSFNVNGVRCGDVVPS--------------------LSGMLRGAEEAKSI 231
           L+  C+S+V +   VNG     ++PS                     S +L  AEE   +
Sbjct: 280 LIMRCVSTVTYRIKVNGDLTDQIIPSRGLRQGDPISPYLFLICAEGFSSLLYAAEERGDL 339

Query: 232 KGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNLDKSVISFS 291
            G+K+ Q  P++SH+ FA D LLLF+  ER A+ ++++LN YE  SGQ VN DKS I FS
Sbjct: 340 SGVKVCQQAPSVSHLLFADDSLLLFKVNERSAQCLQNVLNLYESCSGQIVNKDKSSIMFS 399

Query: 292 LSTNARDRTQVGQILGVQVVACHRQYLGLPSFMPRNKMSSLNFIKDRV 320
            +T+  DR  V +IL +   A + +YLGLP +M R++  +  ++K+RV
Sbjct: 400 KNTSQADRKMVMEILDISTEARNEKYLGLPVYMGRSRAKTFAYLKERV 447

BLAST of Lag0022012 vs. ExPASy TrEMBL
Match: B9FNE2 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_17731 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 3.8e-65
Identity = 128/288 (44.44%), Postives = 184/288 (63.89%), Query Frame = 0

Query: 52  NETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKGVLNEVISHNQSAFIPGR 111
           N+T++ LIPK + P R+ + +PISLC V YKL SKVL NRLK +L ++IS NQSAF+P R
Sbjct: 160 NDTVVTLIPKVQSPERLKDLRPISLCTVVYKLASKVLSNRLKLILPDIISPNQSAFVPQR 219

Query: 112 CVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWVFMEKVMLKVSYAPGWVK 171
            + DN +L YE  H ++ +  GR  +A+LKLDMSK YDRVEW F+EK+M+++ +A GWVK
Sbjct: 220 LITDNVLLAYEMTHFMQTKRTGREGYAALKLDMSKAYDRVEWSFLEKMMVRLGFAEGWVK 279

Query: 172 LVSLCISSVKFSFNVNGVRCGDVVPS--------------------LSGMLRGAEEAKSI 231
           L+  C+S+V +   VNG     ++PS                     S +L  AEE   +
Sbjct: 280 LIMRCVSTVTYRIKVNGDLTDQIIPSRGLRQGDPISPYLFLICAEGFSSLLYAAEERGDL 339

Query: 232 KGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYERGSGQTVNLDKSVISFS 291
            G+K+ Q  P++SH+ FA D LLLF+  ER A+ ++++LN YE  SGQ VN DKS I FS
Sbjct: 340 SGVKVCQQAPSVSHLLFADDSLLLFKVNERSAQCLQNVLNLYESCSGQIVNKDKSSIMFS 399

Query: 292 LSTNARDRTQVGQILGVQVVACHRQYLGLPSFMPRNKMSSLNFIKDRV 320
            +T+  DR  V +IL +   A + +YLGLP +M R++  +  ++K+RV
Sbjct: 400 KNTSQADRKMVMEILDISTEARNEKYLGLPVYMGRSRAKTFAYLKERV 447

BLAST of Lag0022012 vs. ExPASy TrEMBL
Match: A0A5B7BN08 (Reverse transcriptase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_039618 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 3.2e-64
Identity = 133/314 (42.36%), Postives = 190/314 (60.51%), Query Frame = 0

Query: 32  WQQDPGRVLRLI-----EGV-SPALLNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVS 91
           W    G + R+I     +GV S   +N T I LIPK   PR++SE++PISLCNV YK++S
Sbjct: 49  WDVVGGDITRMILDFLNKGVGSLESINYTYIALIPKVNSPRKISEFRPISLCNVVYKIIS 108

Query: 92  KVLVNRLKGVLNEVISHNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMS 151
           K+L NRLK +L  +I+ +QSAF+PGR + DN ++ +E +H L+ + +G+   ++LKLDMS
Sbjct: 109 KILANRLKTILPNIIAESQSAFVPGRLITDNILVAFELIHCLKNKRKGKMGQSALKLDMS 168

Query: 152 KVYDRVEWVFMEKVMLKVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVP--------- 211
           K YDRVEW F+E VML++ +   WV L+  C+S+V FS  +NG   G + P         
Sbjct: 169 KAYDRVEWSFLEAVMLRMGFHQKWVDLIMHCVSTVSFSVLINGDPRGCIKPTRGLRQGDP 228

Query: 212 -----------SLSGMLRGAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEV 271
                      + S +LR +E    I G+ +A+  P +SH+FFA D LL   A E  A  
Sbjct: 229 LSPYLFILCAEAFSALLRKSENENKIHGISVARNAPRVSHLFFADDSLLFANATENQASE 288

Query: 272 VRDILNHYERGSGQTVNLDKSVISFSLSTNARDRTQVGQILGVQVVACHRQYLGLPSFMP 320
           +  I++ Y   SGQ VN +KS ISFS +  A  R Q+ QILGV + + H +YLGLPS + 
Sbjct: 289 ISRIISMYGAASGQQVNFEKSAISFSANVTADRREQIKQILGVSICSIHNKYLGLPSTIG 348

BLAST of Lag0022012 vs. ExPASy TrEMBL
Match: A0A2N9EDY7 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS752 PE=4 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 144/425 (33.88%), Postives = 213/425 (50.12%), Query Frame = 0

Query: 2    GGKNTRWFHTHASSRRRKNEVRDLEDGDGVWQQDPGRVLRLI------------------ 61
            G +NTR+FH  AS RRR+N +  L+D  GVW+++      LI                  
Sbjct: 745  GDRNTRFFHGRASQRRRRNRIMGLQDDQGVWREEKSEFTGLIMQHFESIFRTSIPENIEE 804

Query: 62   -----------------------------------------EGVSPAL------------ 121
                                                     +G+ P              
Sbjct: 805  AVAHVPNLISQEVNTSLTSEFTAQEVELALKQMAPLKAPGPDGMPPLFFQKYWKLVGPEV 864

Query: 122  ----------------LNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKG 181
                            +N T I LIPK + P R++E++PISLCNV+YKL+SKV+ NRLKG
Sbjct: 865  TQGVLSCLNSGRILNNINHTFITLIPKVKNPERITEFRPISLCNVTYKLISKVIANRLKG 924

Query: 182  VLNEVISHNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWV 241
            +L  +IS  QSAF+PGR + DN ++ +E LH +     G+    ++KLDMSK YDRVEW 
Sbjct: 925  ILPSIISEAQSAFVPGRLITDNVLIAFETLHHMHSTKIGKDGAMAMKLDMSKAYDRVEWS 984

Query: 242  FMEKVMLKVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPS----------------- 301
            F+EK+M K+ + P WV L+  CIS+V +S  VNG   G + PS                 
Sbjct: 985  FLEKIMRKMGFHPRWVALIMNCISTVSYSILVNGEPHGFLKPSRGIRQGDPLSPYLFLLC 1044

Query: 302  ---LSGMLRGAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYE 320
               L  ++  A+E  S++GL + + GP I+H+FFA D LL  +A  R+  ++++IL  YE
Sbjct: 1045 AEGLHYLISNAKERGSLQGLSLCRNGPKITHLFFADDSLLFSKATLRECAIIQEILTIYE 1104

BLAST of Lag0022012 vs. ExPASy TrEMBL
Match: A0A2N9IMR2 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS54769 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 3.5e-63
Identity = 145/425 (34.12%), Postives = 213/425 (50.12%), Query Frame = 0

Query: 2   GGKNTRWFHTHASSRRRKNEVRDLEDGDGVWQQDPGRVLRLI------------------ 61
           G +NT++FH  AS RRR+N ++ + D  G+WQ++  +V R+                   
Sbjct: 573 GDRNTKYFHGRASQRRRRNTIKRVRDSAGIWQENEDQVARVFLDYFRTLFTTSNPRNIEE 632

Query: 62  -----------------------------------------EGVSPAL------------ 121
                                                    +G+ P              
Sbjct: 633 AVESTPPIVTQSMNDSLSRDFTAAEAELAISQMAPSTAPGPDGMPPLFYKKFWHIVGPDI 692

Query: 122 ----------------LNETMIVLIPKERRPRRVSEYQPISLCNVSYKLVSKVLVNRLKG 181
                           +N+T I LIPK + P RV+E++PISLCNV YK++SKVLVNRLK 
Sbjct: 693 LKAVLSCLNSDQLLKSINQTYITLIPKVKSPTRVTEFRPISLCNVLYKIISKVLVNRLKP 752

Query: 182 VLNEVISHNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKVYDRVEWV 241
           +L  +IS  QSAF+PG  + DN ++ +E LH++     GR    +LKLDMSK YDRVEW 
Sbjct: 753 ILPHIISKTQSAFVPGCLITDNVLVAFETLHLMHSTKIGRGGAMALKLDMSKAYDRVEWG 812

Query: 242 FMEKVMLKVSYAPGWVKLVSLCISSVKFSFNVNGVRCGDVVPS----------------- 301
           ++EK+M K+ + P W+ L  +CIS V +S  +NG   G + PS                 
Sbjct: 813 YLEKLMEKMGFYPRWISLTMMCISYVSYSILINGEPHGLIKPSRGLRQGDPLSPYLFLLC 872

Query: 302 ---LSGMLRGAEEAKSIKGLKIAQYGPAISHMFFAYDRLLLFRAKERDAEVVRDILNHYE 320
              L  M++ AE  + ++G+ + +YGP I+H+FFA D LL  RA  +D E ++D+L  YE
Sbjct: 873 AEGLHHMIKLAEHQRILQGVSLCRYGPKITHLFFADDSLLFCRATPQDVEKIQDLLGAYE 932

BLAST of Lag0022012 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-11
Identity = 30/83 (36.14%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 88  LVNRLKGVLNEVISHNQSAFIPGRCVVDNAILGYECLHVLRGRSRGRTRWASLKLDMSKV 147
           +V RLK ++  +I   Q++FIPGR   DN +   E +H +R R +G   W  LKLD+ K 
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMR-RKKGVKGWMLLKLDLEKA 60

Query: 148 YDRVEWVFMEKVMLKVSYAPGWV 171
           YDR+ W ++E  ++   +   W+
Sbjct: 61  YDRIRWDYLEDTLISAGFPEVWL 82