Lag0022004 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0022004
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsterol 14-demethylase-like
Locationchr7: 15769065 .. 15772618 (+)
RNA-Seq ExpressionLag0022004
SyntenyLag0022004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCAAGGGGCAAAACCGGCAAGTGGGACGGGCCAAGACCGAAGGGGTCGGGCTCGACCCCGTGCTTGGCCTCGGCCCGCTTGTCTGGCTCCGCTTGGTTCCCACCGCCTCTGGTCGCCTCGGTTCAGTCTGTTTTGTCCCGAGACGCCTCTGAATCCCTAAAAACCCTAAAAGGATGAGCAGGTATTTATATCCCTCTTCGCCACTAAAGAAGGGGACTCAAACTCTAATCCCGAAACTCATTATATATTCTCTACTCTCTCCTCTTGCTCTTACTTTTCCACTCCCCACCGTTCTGCTTGCTTACTTAAGCATCGGAGCCGGTGTGGCGAGCACCACCGGTGTGCAGGTTTACTGTATTGCAGGCCACGTCTTCCCCCTCTTTTACAAATTTTACCGTTGGTGGCACGTGAAAGTCAGGTGAGTTCCTCTGACCAAATTTGACCATCAACATGTACAATATCTCCACTTCTTTCTAAATAAAATAGATTGTTAGTACTTTAAGATAAATTTAAAAAAAAAAAAAAAAAAAACTAAACACAAATATGTCAATTCTTTTAATCAAAATTAGCATAGTTCAATTGACATAAACTATGTGTCATCGATAAGTGAGAGTTTTGAATCCCCTTAGTGAAGTCAAAATATATGTTAAACCTTTTTTTTACTCACCCTAATTTTCTCTTCCAATATTATTGATACAAAATAATGATAATAATCAATATAAATGTGGTGTTTTACTATTTATCCATTTTGTTTACCTTCTCTCTCTTGATCTCTTATTCAACCTTACGCAATCAATTCTTTTGACTTTTAAGTCGAACCTAACTTTTTATTCCTCTTCATAATTAGTGCTAAAAGTAGATGATTGATGACTATTTTTTCTTTAAATGGTTACAATATATATAAAGGATAAAAGTTTATTCAACTTGAAAGTTAATTTATTCTTTTGACTCCTTATCAAGTTAAAGTATATAATAGCCACGTAGCTCAACTGGTATAAGACTTGTACTAATAATCGAAAGGTCTATGATTCGAATCCCCTTGTATTTATTTTCAAAAAAAAAAAATCCATACTAGAATGTCTAACCCATAGAGATCAAATTTAAAAATTAGACTACTATAATTATTATTCCTACATAAATTTAAAAAAGTATTAAATAGATAGTATTCTAGCAATAAACTATTTTGTAAACCAATCCATTTGAATTTTGCAGCCAATAATAAAGTTAAAATCCAAAGAGGATCTCAAGAAAGGGTAAAAAAGAAAAGAAAAAAAAAGTGGGAAAACCATTAGTGAGACCCACTGTTTTACACATGTGATGCCAAACACTCTGAGAGATAAACCCTTAACCCTCACTGCTGTTAATCACACACACTCACTTTAATTAAATCTCTCTCACTCTCTCTCCTCGCTGTTTCTGTCTTCTTCTTCCTCTTCAAATGCCTTCCTGATCTCCACCTTCAATCTCACAATTCAGGTCCCTCAGACCCGAATTCTGCTGCCTTCCGCCTCTAACATCAGCACCCCATTTTCCTTCGATCTGGGTTTCCCAGCAATTGGTCTCCTTCATCTTCCATTTCTCTCTCTCATTTCCTTTCAATTCTCTGTTTGTGTGTTGTTTAATCCCAACCCCAGTTCTGGAATCTTTTGCAGGTTGTGTGCGATGGAGCCGGAGAACAAGTTATTCAATGTGGGTCTCCTCGTAGTGGCGACTTTAGTCCTTGCCAAGGTCATTTCTGCTGTTCTGTATTCCAGATCTAAGAAACGGGCTCCTCCCACTGTCAAGGGGCTGCCCCTTGTTGGGGGGTTGCTCCGCTTTCTTAAGGGTCCGATCGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACTTATTGCACAAGAAGATTACCTTCTTGATTGGGCCTGAGGTTTCTGCGCATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGCCCTGGCGTTGTGTTTGATGTGGATTACTCCGTCCGCCAAGAGCAGTTCAGGTTCTTCACTGAAGCTCTCAGAGTCACGAAATTGAAAGGCTATGTGGATCAGATGGTTAAAGAAGCAGAGGTCAGTATCAACCCCCTTGAAATATCTAAATGGATAAGATATCATTAAATGCAGCCTCAGATAAAGAATCTGGATATTAGGATTCTGTGAGAACTTGTGCTCTGCTTGTTAACTTTGAAGTTTTACTAAATTTTTGGAAAAGTTTCTCCCTCTCTCTAGCTGTCATGATTGATGTTCAGATGATGAAAAATGAATAATAAAGAAGTAAAATGTAGCCCAGCATCCTTGCTAATGGGAATGCATGTTAAATGTTAACAACAGTCCTTAGAGATTTGTCTTCTTAATGTATAGTCTGAACTTCCATGTATCTCTTAAAACGTCTAGCTCCTGATGCTTGTGTTCAAATCTTTGAGTCTCATTCTCTTCGTTTTACAGTATTCTATAAATCTGCTTAACCTTTGGGATGTGATGGGGTATTTGAGCAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTCCTTGGTAGAGAGGTTCGTGATAAGCTTTTTGCTGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCGGCTCATCGTCGCCGTGATCAAGCCCGCAGCAAGCTTGCAGATATCTTTGCGAAAATCATAGCCTCCAGAAAATGCAATGGAAGTTTAGAAAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACAACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCATATCTCCTACGCCACAAAGAGTACATGTCTGCTGTTGTAGAGGAGCAGCAAAAGCTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGACAACCTCCATAGGTGCATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTCAGTGTGACAACTAGAGAGGGGAAAGAATTTGTTATTCCAAAGGGTCATATAATTGCAACATCTCCAGCTTTCGCCAATCGACTTCCTCATGTCTACAACGATCCAGACAGGTATGATCCAGACAGGTTTGTCCCTGGAAGAGATGAAGACAAGGCAGCTGGGCAATTCTCCTACATCTCATTTGGAGGCGGCAGGCACGGGTGCCTTGGCGAGCCCTTTGCTTACTTACAGATAAAGGCAATATGGAGTCAGTTACTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTAGGTGTTAAAGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGTTAA

mRNA sequence

ATGGATCCAAGGGGCAAAACCGGCAAGTGGGACGGGCCAAGACCGAAGGGGTCGGGCTCGACCCCGTGCTTGGCCTCGGCCCGCTTGTCTGGCTCCGCTTGGTTCCCACCGCCTCTGGTCGCCTCGAAGGGGACTCAAACTCTAATCCCGAAACTCATTATATATTCTCTACTCTCTCCTCTTGCTCTTACTTTTCCACTCCCCACCGTTCTGCTTGCTTACTTAAGCATCGGAGCCGGTGTGGCGAGCACCACCGGTGTGCAGGTTTACTGTATTGCAGGCCACGTCTTCCCCCTCTTTTACAAATTTTACCGTTGGTGGCACGTGAAAGTCAGGTTGTGTGCGATGGAGCCGGAGAACAAGTTATTCAATGTGGGTCTCCTCGTAGTGGCGACTTTAGTCCTTGCCAAGGTCATTTCTGCTGTTCTGTATTCCAGATCTAAGAAACGGGCTCCTCCCACTGTCAAGGGGCTGCCCCTTGTTGGGGGGTTGCTCCGCTTTCTTAAGGGTCCGATCGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACTTATTGCACAAGAAGATTACCTTCTTGATTGGGCCTGAGGTTTCTGCGCATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGCCCTGGCGTTGTGTTTGATGTGGATTACTCCGTCCGCCAAGAGCAGTTCAGGTTCTTCACTGAAGCTCTCAGAGTCACGAAATTGAAAGGCTATGTGGATCAGATGGTTAAAGAAGCAGAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTCCTTGGTAGAGAGGTTCGTGATAAGCTTTTTGCTGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCGGCTCATCGTCGCCGTGATCAAGCCCGCAGCAAGCTTGCAGATATCTTTGCGAAAATCATAGCCTCCAGAAAATGCAATGGAAGTTTAGAAAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACAACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCATATCTCCTACGCCACAAAGAGTACATGTCTGCTGTTGTAGAGGAGCAGCAAAAGCTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGACAACCTCCATAGGTGCATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTCAGTGTGACAACTAGAGAGGGGAAAGAATTTGTTATTCCAAAGGGTCATATAATTGCAACATCTCCAGCTTTCGCCAATCGACTTCCTCATGTCTACAACGATCCAGACAGGTATGATCCAGACAGGTTTGTCCCTGGAAGAGATGAAGACAAGGCAGCTGGGCAATTCTCCTACATCTCATTTGGAGGCGGCAGGCACGGGTGCCTTGGCGAGCCCTTTGCTTACTTACAGATAAAGGCAATATGGAGTCAGTTACTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTAGGTGTTAAAGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGTTAA

Coding sequence (CDS)

ATGGATCCAAGGGGCAAAACCGGCAAGTGGGACGGGCCAAGACCGAAGGGGTCGGGCTCGACCCCGTGCTTGGCCTCGGCCCGCTTGTCTGGCTCCGCTTGGTTCCCACCGCCTCTGGTCGCCTCGAAGGGGACTCAAACTCTAATCCCGAAACTCATTATATATTCTCTACTCTCTCCTCTTGCTCTTACTTTTCCACTCCCCACCGTTCTGCTTGCTTACTTAAGCATCGGAGCCGGTGTGGCGAGCACCACCGGTGTGCAGGTTTACTGTATTGCAGGCCACGTCTTCCCCCTCTTTTACAAATTTTACCGTTGGTGGCACGTGAAAGTCAGGTTGTGTGCGATGGAGCCGGAGAACAAGTTATTCAATGTGGGTCTCCTCGTAGTGGCGACTTTAGTCCTTGCCAAGGTCATTTCTGCTGTTCTGTATTCCAGATCTAAGAAACGGGCTCCTCCCACTGTCAAGGGGCTGCCCCTTGTTGGGGGGTTGCTCCGCTTTCTTAAGGGTCCGATCGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACTTATTGCACAAGAAGATTACCTTCTTGATTGGGCCTGAGGTTTCTGCGCATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGCCCTGGCGTTGTGTTTGATGTGGATTACTCCGTCCGCCAAGAGCAGTTCAGGTTCTTCACTGAAGCTCTCAGAGTCACGAAATTGAAAGGCTATGTGGATCAGATGGTTAAAGAAGCAGAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTCCTTGGTAGAGAGGTTCGTGATAAGCTTTTTGCTGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCGGCTCATCGTCGCCGTGATCAAGCCCGCAGCAAGCTTGCAGATATCTTTGCGAAAATCATAGCCTCCAGAAAATGCAATGGAAGTTTAGAAAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACAACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCATATCTCCTACGCCACAAAGAGTACATGTCTGCTGTTGTAGAGGAGCAGCAAAAGCTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGACAACCTCCATAGGTGCATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTCAGTGTGACAACTAGAGAGGGGAAAGAATTTGTTATTCCAAAGGGTCATATAATTGCAACATCTCCAGCTTTCGCCAATCGACTTCCTCATGTCTACAACGATCCAGACAGGTATGATCCAGACAGGTTTGTCCCTGGAAGAGATGAAGACAAGGCAGCTGGGCAATTCTCCTACATCTCATTTGGAGGCGGCAGGCACGGGTGCCTTGGCGAGCCCTTTGCTTACTTACAGATAAAGGCAATATGGAGTCAGTTACTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTAGGTGTTAAAGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGTTAA

Protein sequence

MDPRGKTGKWDGPRPKGSGSTPCLASARLSGSAWFPPPLVASKGTQTLIPKLIIYSLLSPLALTFPLPTVLLAYLSIGAGVASTTGVQVYCIAGHVFPLFYKFYRWWHVKVRLCAMEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSVS
Homology
BLAST of Lag0022004 vs. NCBI nr
Match: KAG6597695.1 (Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 945.3 bits (2442), Expect = 2.6e-271
Identity = 464/490 (94.69%), Postives = 479/490 (97.76%), Query Frame = 0

Query: 111 VRLCAMEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKG 170
           +RLCAMEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG++RFLKG
Sbjct: 78  LRLCAMEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKG 137

Query: 171 PIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGP 230
           PIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGP
Sbjct: 138 PIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGP 197

Query: 231 GVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHL 290
           GVVFDVDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHL
Sbjct: 198 GVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHL 257

Query: 291 IILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLAD 350
           IILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLAD
Sbjct: 258 IILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLAD 317

Query: 351 IFAKIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTW 410
           IFAKIIASRKCNGS ENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTW
Sbjct: 318 IFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTW 377

Query: 411 TGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMR 470
           TGAYLLRHKEYMSAV+EEQQKLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMR
Sbjct: 378 TGAYLLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMR 437

Query: 471 SSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAG 530
           SSHTDFSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF P RDEDKAAG
Sbjct: 438 SSHTDFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAG 497

Query: 531 QFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVM 590
            FSYISFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVM
Sbjct: 498 PFSYISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVM 557

Query: 591 VRYKRRKLSV 601
           VRYKRRKLSV
Sbjct: 558 VRYKRRKLSV 567

BLAST of Lag0022004 vs. NCBI nr
Match: XP_022932660.1 (sterol 14-demethylase-like [Cucurbita moschata] >XP_022932661.1 sterol 14-demethylase-like [Cucurbita moschata] >XP_022932662.1 sterol 14-demethylase-like [Cucurbita moschata] >KAG7029139.1 Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 936.0 bits (2418), Expect = 1.6e-268
Identity = 460/485 (94.85%), Postives = 474/485 (97.73%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRKCNGS ENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQKLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF P RDEDKAAG FSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSV 601
           RKLSV
Sbjct: 481 RKLSV 485

BLAST of Lag0022004 vs. NCBI nr
Match: XP_023539138.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 934.5 bits (2414), Expect = 4.6e-268
Identity = 460/485 (94.85%), Postives = 473/485 (97.53%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRS KRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSNKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRKCNGS ENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQKLM KHGDK DHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF P RDEDKAAG FSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSV 601
           RKLSV
Sbjct: 481 RKLSV 485

BLAST of Lag0022004 vs. NCBI nr
Match: XP_022980879.1 (sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 932.6 bits (2409), Expect = 1.8e-267
Identity = 460/486 (94.65%), Postives = 473/486 (97.33%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK  NVGLLVVATL+LAKVISAVL SRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRK +GS ENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF PGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSVS 602
           RKLSV+
Sbjct: 481 RKLSVN 486

BLAST of Lag0022004 vs. NCBI nr
Match: XP_023527569.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 932.2 bits (2408), Expect = 2.3e-267
Identity = 460/486 (94.65%), Postives = 473/486 (97.33%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK  NVGLLVVATL+LAKVISAVL SRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRK +GS ENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF PGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSVS 602
           RKLSV+
Sbjct: 481 RKLSVN 486

BLAST of Lag0022004 vs. ExPASy Swiss-Prot
Match: Q9SAA9 (Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.8e-238
Identity = 402/486 (82.72%), Postives = 445/486 (91.56%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRS-KKRAPPTVKGL-PLVGGLLRFLKGPIV 175
           ++ ENKL   GL++VATLV+AK+I +   S S KKR PPT+K   PLVG L++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 176 MLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 235
           MLREEYPKLGSVFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 236 FDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIIL 295
           FDVDYSVRQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 296 TASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFA 355
           TASRCLLGREVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 356 KIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGA 415
           KII SRK +G  ENDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 416 YLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSH 475
           YL+R+KEY SA ++EQ+ L+ KHGDKIDHDILSEMD L+RCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 476 TDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFS 535
           +DFSVT R+GK + IPKGHI+ATSPAFANRLPH++ DPD YDP+RF PGR+EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 536 YISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRY 595
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 596 KRRKLS 600
           KRR+LS
Sbjct: 483 KRRQLS 488

BLAST of Lag0022004 vs. ExPASy Swiss-Prot
Match: P93846 (Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.5e-221
Identity = 366/484 (75.62%), Postives = 428/484 (88.43%), Query Frame = 0

Query: 118 PENKLFNVGL-LVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVMLR 177
           P+ +    GL LVVAT++  K++ +      KKR PPT+ G P+VGGL++F++GPI M+R
Sbjct: 7   PQQQRLMAGLALVVATVIFLKLLLSFRSGGGKKRLPPTIPGAPVVGGLVKFMRGPIPMIR 66

Query: 178 EEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDV 237
           E+Y  LGSVFT+ ++ ++ITFLIGPEVSAHFFK +E+++SQQEVY+FNVPTFGPGVVFDV
Sbjct: 67  EQYAALGSVFTVPIITRRITFLIGPEVSAHFFKGNEAEMSQQEVYRFNVPTFGPGVVFDV 126

Query: 238 DYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTAS 297
           DYSVRQEQFRFFTEALR  KL+ YVDQMV EAE++FSKWG+SG VDLKYELEHLIILTAS
Sbjct: 127 DYSVRQEQFRFFTEALRANKLRSYVDQMVAEAEEYFSKWGESGTVDLKYELEHLIILTAS 186

Query: 298 RCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKII 357
           RCLLGREVR+KLF DVSALFHDLDNG+ PISV+FPYLPIPAH+RRD+AR++LA+IFA II
Sbjct: 187 RCLLGREVREKLFDDVSALFHDLDNGIQPISVLFPYLPIPAHKRRDKARARLAEIFATII 246

Query: 358 ASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLL 417
            SRK +G  E DMLQCFIDSKYK+GRPTT+ EVTGLLIAALFAGQHTSSITSTWTGAY+L
Sbjct: 247 KSRKASGQSEEDMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYML 306

Query: 418 RHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDF 477
           R K+Y +  VEEQ+ +M +HGDKIDHDIL+EMD L+RCIKEALRLHPPLIML+R SH+DF
Sbjct: 307 RFKQYFAEAVEEQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDF 366

Query: 478 SVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYIS 537
           +VTT+EGKE+ IPKGHI+ATSP+FANRLPH+Y +PD YDPDRF PGR+EDKAAG FSYIS
Sbjct: 367 TVTTKEGKEYDIPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYIS 426

Query: 538 FGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRR 597
           FGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL+SPFPE DWNAMVVG+KG+VMV YKRR
Sbjct: 427 FGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRR 486

Query: 598 KLSV 601
           KL V
Sbjct: 487 KLVV 490

BLAST of Lag0022004 vs. ExPASy Swiss-Prot
Match: P93596 (Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP51 PE=2 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 7.0e-219
Identity = 361/451 (80.04%), Postives = 409/451 (90.69%), Query Frame = 0

Query: 150 RAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFK 209
           R PPT+ G P+VGGLLRFL+GPI ++R EY +LG VFT+ +L ++ITFLIGP+VSAHFFK
Sbjct: 1   RPPPTIPGAPVVGGLLRFLRGPIPLIRAEYARLGPVFTVPILTRRITFLIGPDVSAHFFK 60

Query: 210 ASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAE 269
           ++ESD+SQQEVY+FNVPTFGPGVVFDVDY VRQEQFRFFTEALR  KL+ YVDQMV EAE
Sbjct: 61  SNESDMSQQEVYRFNVPTFGPGVVFDVDYQVRQEQFRFFTEALRANKLRSYVDQMVAEAE 120

Query: 270 DFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVM 329
           ++FSKWG+SG VDLKYELEHLIILTASRCLLGREVR+KLF DVSALFHDLDNGMLPISV+
Sbjct: 121 EYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREKLFDDVSALFHDLDNGMLPISVI 180

Query: 330 FPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEV 389
           FPYLPIPAHRRRDQAR++LA+IFA II SRK +G  E DMLQCFIDSKYK+GR TT+SEV
Sbjct: 181 FPYLPIPAHRRRDQARTRLAEIFATIIKSRKASGQSEEDMLQCFIDSKYKNGRQTTESEV 240

Query: 390 TGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMD 449
           TGLLIAALFAGQHTSSITSTWTGAYLL+ ++Y +  VEEQ+++M +HGDKIDHDIL+EMD
Sbjct: 241 TGLLIAALFAGQHTSSITSTWTGAYLLKFQQYFAEAVEEQKEVMKRHGDKIDHDILAEMD 300

Query: 450 NLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYN 509
            L+RCIKEALRLHPPLIML+R SH+DFSVTTREGKEF IPKGHI+ATSPAFANRLPH++ 
Sbjct: 301 VLYRCIKEALRLHPPLIMLLRQSHSDFSVTTREGKEFDIPKGHIVATSPAFANRLPHIFK 360

Query: 510 DPDRYDPDRFVPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELEL 569
           +PD YDPDRF  GR+EDK AG FSYISFGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL
Sbjct: 361 NPDSYDPDRFAAGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFEL 420

Query: 570 ISPFPEIDWNAMVVGVKGKVMVRYKRRKLSV 601
           +SPFPE DWNAMVVG+KG+VMV YKRRKL V
Sbjct: 421 VSPFPENDWNAMVVGIKGEVMVNYKRRKLIV 451

BLAST of Lag0022004 vs. ExPASy Swiss-Prot
Match: Q1ZXH9 (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN=cyp51 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.3e-103
Identity = 203/473 (42.92%), Postives = 292/473 (61.73%), Query Frame = 0

Query: 130 VATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLN 189
           +A LV+A ++    + +S    PP V+ +P +G   +F K P+ ++R  Y +LG +FTL+
Sbjct: 5   IAVLVIA-ILVIFAFKKSPSNIPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIFTLH 64

Query: 190 LLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFT 249
           L+  K+TF++GPE  A FF+ ++ +LS +E Y+F  P FG GVV+D +  +  EQ RF  
Sbjct: 65  LMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQLRFVK 124

Query: 250 EALRVTKLKGYVDQMVKEAEDFF-SKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKL 309
             L +++LK  V  + +E E +F +KWGDSGE+DL YE+  L ILTASRCL+G+ +   L
Sbjct: 125 NGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKSINKSL 184

Query: 310 --FADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIAS-RKCNGSL 369
                ++ L+H+L+ G+ PIS  FP LP+P+ ++RD AR+K+A IF  II   R+     
Sbjct: 185 GQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRRSTDDS 244

Query: 370 ENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAV 429
            +D+L   ++SKYKDG    D ++ GL+I  LFAGQHTSSIT T+T  YLL + EY    
Sbjct: 245 VDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLEYFDET 304

Query: 430 VEEQQKLMGKHGD-KIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGK 489
            ++   ++ K    +I+ D L  M+ L   I+E LRLHPPLI LMR       +T  E K
Sbjct: 305 QKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRK-----VMTPMEYK 364

Query: 490 EFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYISFGGGRHGC 549
              IP GHI+A SP    RLP VY +PD ++P RF     EDK    FS+I+FGGG+HGC
Sbjct: 365 GKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRF---DVEDKT--PFSFIAFGGGKHGC 424

Query: 550 LGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRK 598
            GE F  LQIK IW+ L   + LE + P P  D+ ++V G KG  MV+Y +++
Sbjct: 425 PGENFGILQIKTIWTVLSTKYNLE-VGPVPPTDFTSLVAGPKGPCMVKYSKKQ 465

BLAST of Lag0022004 vs. ExPASy Swiss-Prot
Match: Q4PJW3 (Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.5e-91
Identity = 180/451 (39.91%), Postives = 265/451 (58.76%), Query Frame = 0

Query: 149 KRAPPTVKGLPLVGGLLRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFF 208
           K  P  V  +P +G  + F K PI  L + Y K G VF+  ++ K  T+L+G E +A  F
Sbjct: 58  KSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEKYGPVFSFTMVGKTFTYLLGSEAAALLF 117

Query: 209 KASESDLSQQEVY-QFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKE 268
            +   DL+ +EVY +   P FG GV +DV  +V  EQ +     L +   + +V  + KE
Sbjct: 118 NSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNTVFLEQKKMLKSGLNIAHFRQHVSIIEKE 177

Query: 269 AEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPIS 328
            +++F  WG+SGE +L   L  LIILTAS CL G+E+R +L   V+ L+ DLD G    +
Sbjct: 178 TKEYFKSWGESGEKNLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAA 237

Query: 329 VMFP-YLPIPAHRRRDQARSKLADIFAKIIASRKCNGSLENDMLQCFIDSKYKDGRPTTD 388
            + P +LP+P+ RRRD+A  ++ +IF K I  R+ +G   +D+LQ  ++S YKDGRP TD
Sbjct: 238 WLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYKDGRPLTD 297

Query: 389 SEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILS 448
            EV G+LI  L AGQHTSS TS W G +L R K        EQ+ + G++   + +D L 
Sbjct: 298 DEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQLK 357

Query: 449 EMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPH 508
           +++ L RCIKE LRL PP++ +MR + T  +V       + IP GH +  SP    RL  
Sbjct: 358 DLNLLDRCIKETLRLRPPIMTMMRLAKTPLTVA-----GYTIPPGHQVCVSPTVNQRLKD 417

Query: 509 VYNDPDRYDPDRFVPGRDEDKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFE 568
            + +   ++PDR++   D   +  +F+Y+ FG GRH C+GE FAY+QIK IWS +LR +E
Sbjct: 418 SWVERLDFNPDRYL--EDSPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYE 477

Query: 569 LELISP-FPEIDWNAMVVGVKGKVMVRYKRR 597
            +LI   FP +++  M +    K ++RYKRR
Sbjct: 478 FDLIDGYFPTVNYTTM-IHTPEKPIIRYKRR 500

BLAST of Lag0022004 vs. ExPASy TrEMBL
Match: A0A6J1F2S4 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111439144 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 7.7e-269
Identity = 460/485 (94.85%), Postives = 474/485 (97.73%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG++RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRKCNGS ENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQKLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTT+EGK + IPKGHIIATSPAFANRLPHVY DPDRYDPDRF P RDEDKAAG FSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSV 601
           RKLSV
Sbjct: 481 RKLSV 485

BLAST of Lag0022004 vs. ExPASy TrEMBL
Match: A0A6J1J0F8 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 8.5e-268
Identity = 460/486 (94.65%), Postives = 473/486 (97.33%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK  NVGLLVVATL+LAKVISAVL SRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRK +GS ENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF PGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSVS 602
           RKLSV+
Sbjct: 481 RKLSVN 486

BLAST of Lag0022004 vs. ExPASy TrEMBL
Match: A0A6J1I3Z5 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111470769 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.9e-267
Identity = 458/485 (94.43%), Postives = 473/485 (97.53%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK FNVGLLVVATLVLAKVIS+VL SRSKKRAPPTV+GLPLVGG+LRFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMLRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           REEYPKLGSVF LNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFILNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRVTKLKGYVDQMVKEAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRKCNGS ENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQ+KLM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHT+
Sbjct: 301 LRHKEYMSAVIEEQKKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTN 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSV T+EGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF P RDEDKAAG FSYI
Sbjct: 361 FSVITKEGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSV 601
           RKLSV
Sbjct: 481 RKLSV 485

BLAST of Lag0022004 vs. ExPASy TrEMBL
Match: A0A6J1GUN6 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.2e-266
Identity = 458/486 (94.24%), Postives = 472/486 (97.12%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVML 175
           MEP+NK  NVGLLVVATL+LAKVISAVL SRS KRAPPTVKG PLVGGL+RFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 176 REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 235
           R+EYP+LG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 236 VDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTA 295
           VDYS+RQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 296 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 355
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 356 IASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 415
           IASRK +GS ENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 416 LRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 475
           LRHKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 476 FSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYI 535
           FSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF PGRDEDKAAG FSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 536 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 595
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 596 RKLSVS 602
           RKLSV+
Sbjct: 481 RKLSVN 486

BLAST of Lag0022004 vs. ExPASy TrEMBL
Match: A0A0A0KYP3 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 3.6e-266
Identity = 457/487 (93.84%), Postives = 473/487 (97.13%), Query Frame = 0

Query: 116 MEPEN-KLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLRFLKGPIVM 175
           MEP+N K FNVGLLV+ATL+LAK+ISA+L SRS KR PPTVKG+P +GGLLRFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 176 LREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 235
           LR+EYPKLGSVFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 236 DVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILT 295
           DVDYS+RQEQFRFFTEALRVTKLKGYVDQMV+EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 296 ASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 355
           ASRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 356 IIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 415
           IIASRKCNGS +NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 416 LLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHT 475
           LL HKEYMSAV+EEQQ+LMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 476 DFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSY 535
           DFSVTTREGKE+ IPKGHIIATSPAFANRLPHVY DPDRYDPDRF PGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 536 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 595
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 596 RRKLSVS 602
           RRKLSVS
Sbjct: 481 RRKLSVS 487

BLAST of Lag0022004 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 824.7 bits (2129), Expect = 4.8e-239
Identity = 402/486 (82.72%), Postives = 445/486 (91.56%), Query Frame = 0

Query: 116 MEPENKLFNVGLLVVATLVLAKVISAVLYSRS-KKRAPPTVKGL-PLVGGLLRFLKGPIV 175
           ++ ENKL   GL++VATLV+AK+I +   S S KKR PPT+K   PLVG L++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 176 MLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 235
           MLREEYPKLGSVFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 236 FDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIIL 295
           FDVDYSVRQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 296 TASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFA 355
           TASRCLLGREVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 356 KIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGA 415
           KII SRK +G  ENDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 416 YLLRHKEYMSAVVEEQQKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSH 475
           YL+R+KEY SA ++EQ+ L+ KHGDKIDHDILSEMD L+RCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 476 TDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDEDKAAGQFS 535
           +DFSVT R+GK + IPKGHI+ATSPAFANRLPH++ DPD YDP+RF PGR+EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 536 YISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRY 595
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 596 KRRKLS 600
           KRR+LS
Sbjct: 483 KRRQLS 488

BLAST of Lag0022004 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 117.9 bits (294), Expect = 2.9e-26
Identity = 106/469 (22.60%), Postives = 198/469 (42.22%), Query Frame = 0

Query: 138 VISAVLYSRSKKRAPPTVKGLPLVGGLLR-FLKGPIVMLREEYPKLGSVFTLNLLHKKIT 197
           +IS   +  SK   PP   G P VG   + + + P V  + +  + GSVF  ++L     
Sbjct: 23  LISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCV 82

Query: 198 FLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTK 257
            +  PE +A F   ++S L +           G   +F        +  +    A     
Sbjct: 83  MISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPES 142

Query: 258 LKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF-ADVSAL 317
           ++  V  +   A+D    W +   ++   E++      A   + G++  + L+  D+   
Sbjct: 143 IRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRC 202

Query: 318 FHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSLENDMLQCFID 377
           ++ L+ G   + V    LP     +  +AR +L+ I A+I++ R+ NGS  ND+L  F+ 
Sbjct: 203 YYILEKGYNSMPV---NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMG 262

Query: 378 SKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLM-- 437
            K       TD ++   +I  +FA + T++   +W   YL  +   + AV EEQ  +   
Sbjct: 263 DK----EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKD 322

Query: 438 GKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHI 497
            + G+ +      +M    R I+E LR+   L    R +  D      E + ++IPKG  
Sbjct: 323 KEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWK 382

Query: 498 IATSPAFAN--RLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYISFGGGRHGCLGEPFAY 557
           +   P F N      ++++P ++DP RF      + A    +++ FG G H C G   A 
Sbjct: 383 VL--PLFRNIHHSADIFSNPGKFDPSRF------EVAPKPNTFMPFGNGTHSCPGNELAK 442

Query: 558 LQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSV 601
           L++  +   L   +   ++     I +    +   G  +V  ++ ++ V
Sbjct: 443 LEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARKPEIEV 467

BLAST of Lag0022004 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 114.0 bits (284), Expect = 4.2e-25
Identity = 102/434 (23.50%), Postives = 185/434 (42.63%), Query Frame = 0

Query: 138 VISAVLYSRSKKRAPPTVKGLPLVGGLLR-FLKGPIVMLREEYPKLGSVFTLNLLHKKIT 197
           +IS   +  SK   PP   G P VG   + + + P V  + +  + GSVF  ++L     
Sbjct: 23  LISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCV 82

Query: 198 FLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSVRQEQFRFFTEALRVTK 257
            +  PE +A F   ++S L +           G   +F        +  +    A     
Sbjct: 83  MISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPES 142

Query: 258 LKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF-ADVSAL 317
           ++  V  +   A+D    W +   ++   E++      A   + G++  + L+  D+   
Sbjct: 143 IRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRC 202

Query: 318 FHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSLENDMLQCFID 377
           ++ L+ G   + V    LP     +  +AR +L+ I A+I++ R+ NGS  ND+L  F+ 
Sbjct: 203 YYILEKGYNSMPV---NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMG 262

Query: 378 SKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVVEEQQKLM-- 437
            K       TD ++   +I  +FA + T++   +W   YL  +   + AV EEQ  +   
Sbjct: 263 DK----EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKD 322

Query: 438 GKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEFVIPKGHI 497
            + G+ +      +M    R I+E LR+   L    R +  D      E + ++IPKG  
Sbjct: 323 KEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWK 382

Query: 498 IATSPAFAN--RLPHVYNDPDRYDPDRFVPGRDEDKAAGQFSYISFGGGRHGCLGEPFAY 557
           +   P F N      ++++P ++DP RF      + A    +++ FG G H C G   A 
Sbjct: 383 VL--PLFRNIHHSADIFSNPGKFDPSRF------EVAPKPNTFMPFGNGTHSCPGNELAK 432

Query: 558 LQIKAIWSQLLRNF 566
           L++  +   L   +
Sbjct: 443 LEMSIMIHHLTTKY 432

BLAST of Lag0022004 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 110.5 bits (275), Expect = 4.6e-24
Identity = 107/482 (22.20%), Postives = 187/482 (38.80%), Query Frame = 0

Query: 119 ENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLR-FLKGPIVMLRE 178
           E  L  + ++VV  ++L K    + +   + R PP   GLP +G  LR + + P      
Sbjct: 19  EPALITLTIVVVVVVLLFK--WWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNSFFAT 78

Query: 179 EYPKLGSVFTLNLLHKKITFLIGPEVS-------AHFFKASESDLSQQEVYQFNVPTFGP 238
              K G +F  ++L      +  PE +       AH FK +     ++ +        GP
Sbjct: 79  RQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMI--------GP 138

Query: 239 GVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHL 298
             +F           R    +   + L+  V  +        S W     ++    ++  
Sbjct: 139 EALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRY 198

Query: 299 IILTASRCLLGREVRDKLFADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKL 358
               A     G +        +  L+  L+ G   +P+      LP     +  +AR +L
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD-----LPGTLFHKSMKARIEL 258

Query: 359 ADIFAKIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITS 418
           ++   K+I  R+ NG  E  +L   + +K +     +DS++   +I  +FA   T++   
Sbjct: 259 SEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 419 TWTGAYLLRHKEYMSAVVEEQ---QKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPL 478
           TW   YL  H   +  V  EQ   ++ + K   +I  +   +M    R I+E LR    L
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 479 IMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDE 538
               R +  D      E   ++IPKG  +       +     + DP+++DP RF      
Sbjct: 379 SFTFREAVQDV-----EYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRF------ 438

Query: 539 DKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGV 588
           + A   ++Y+ FG G H C G   A L++  +   L  +F  E+I     I +    V  
Sbjct: 439 EVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPK 474

BLAST of Lag0022004 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 110.5 bits (275), Expect = 4.6e-24
Identity = 107/482 (22.20%), Postives = 187/482 (38.80%), Query Frame = 0

Query: 119 ENKLFNVGLLVVATLVLAKVISAVLYSRSKKRAPPTVKGLPLVGGLLR-FLKGPIVMLRE 178
           E  L  + ++VV  ++L K    + +   + R PP   GLP +G  LR + + P      
Sbjct: 19  EPALITLTIVVVVVVLLFK--WWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNSFFAT 78

Query: 179 EYPKLGSVFTLNLLHKKITFLIGPEVS-------AHFFKASESDLSQQEVYQFNVPTFGP 238
              K G +F  ++L      +  PE +       AH FK +     ++ +        GP
Sbjct: 79  RQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMI--------GP 138

Query: 239 GVVFDVDYSVRQEQFRFFTEALRVTKLKGYVDQMVKEAEDFFSKWGDSGEVDLKYELEHL 298
             +F           R    +   + L+  V  +        S W     ++    ++  
Sbjct: 139 EALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRY 198

Query: 299 IILTASRCLLGREVRDKLFADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKL 358
               A     G +        +  L+  L+ G   +P+      LP     +  +AR +L
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD-----LPGTLFHKSMKARIEL 258

Query: 359 ADIFAKIIASRKCNGSLENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITS 418
           ++   K+I  R+ NG  E  +L   + +K +     +DS++   +I  +FA   T++   
Sbjct: 259 SEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 419 TWTGAYLLRHKEYMSAVVEEQ---QKLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPL 478
           TW   YL  H   +  V  EQ   ++ + K   +I  +   +M    R I+E LR    L
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 479 IMLMRSSHTDFSVTTREGKEFVIPKGHIIATSPAFANRLPHVYNDPDRYDPDRFVPGRDE 538
               R +  D      E   ++IPKG  +       +     + DP+++DP RF      
Sbjct: 379 SFTFREAVQDV-----EYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRF------ 438

Query: 539 DKAAGQFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGV 588
           + A   ++Y+ FG G H C G   A L++  +   L  +F  E+I     I +    V  
Sbjct: 439 EVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPK 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6597695.12.6e-27194.69Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932660.11.6e-26894.85sterol 14-demethylase-like [Cucurbita moschata] >XP_022932661.1 sterol 14-demeth... [more]
XP_023539138.14.6e-26894.85sterol 14-demethylase-like [Cucurbita pepo subsp. pepo][more]
XP_022980879.11.8e-26794.65sterol 14-demethylase-like [Cucurbita maxima][more]
XP_023527569.12.3e-26794.65sterol 14-demethylase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SAA96.8e-23882.72Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1[more]
P938461.5e-22175.62Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1[more]
P935967.0e-21980.04Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP... [more]
Q1ZXH91.3e-10342.92Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN... [more]
Q4PJW31.5e-9139.91Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2S47.7e-26994.85sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111439144 PE=3 SV... [more]
A0A6J1J0F88.5e-26894.65sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1[more]
A0A6J1I3Z51.9e-26794.43sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111470769 PE=3 SV=1[more]
A0A6J1GUN61.2e-26694.24sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV... [more]
A0A0A0KYP33.6e-26693.84Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G11680.14.8e-23982.72CYTOCHROME P450 51G1 [more]
AT4G19230.12.9e-2622.60cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT4G19230.24.2e-2523.50cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT2G29090.14.6e-2422.20cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.24.6e-2422.20cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 390..416
score: 37.32
coord: 173..196
score: 35.1
coord: 544..562
score: 43.52
coord: 528..544
score: 34.39
coord: 448..464
score: 46.61
coord: 502..520
score: 27.94
coord: 151..168
score: 24.57
coord: 486..500
score: 35.9
IPR001128Cytochrome P450PRINTSPR00385P450coord: 399..416
score: 32.57
coord: 544..555
score: 38.9
coord: 453..464
score: 40.69
coord: 535..544
score: 40.95
IPR001128Cytochrome P450PFAMPF00067p450coord: 152..571
e-value: 2.2E-65
score: 221.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 142..600
e-value: 1.5E-121
score: 408.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 147..596
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 125..596
e-value: 1.3E-34
score: 117.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR24286:SF251STEROL 14-DEMETHYLASEcoord: 118..595
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 118..595
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 537..546

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0022004.1Lag0022004.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0016126 sterol biosynthetic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen