Lag0022001 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0022001
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA polymerase epsilon catalytic subunit
Location	chr7: 15705583 .. 15706983 (-)
RNA-Seq Expression	Lag0022001
Synteny	Lag0022001

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTGGTATTTTAGTAGAAACAAAATTAACTACGAACGGTCCTATTGCTTCCGCTGTAACGGAATTGGGGAATTCCAATCCTTCACCACCCTCGTCTTTTTCGCCCCTGCGCACCAAATCTCCCGCACCATCGGTTCTCCTCCGTCGCCGTTGTCATTTATCTCGCGTCGCCGTCGTTGTTTTGCTCGTGCTTCCGTCGTCGTTCCACTCTGTAGTCATCGTCTTTTCTCGCTTCAACAGGTTCGCTTCTTCTTCTTCTACTTATTTATCATGAACGCATAATTTGATCAATGATACACCCTTACTTTTCAGTTATTTAACTTAATCTTTTTTTAACTAGAAAAATTTATTTAATCACCATTGCAGTTAGAGCTTCCCAAACTGTGGTTTGCCCATATGCGAGAGGTGAAACCTGGTATCTACGTCACCTACAATGGCGACTATTTTGACTGGCCATTCCTTGAAAGTAGAGCAACGTATCATGGATTTAAAATGAGTGATGTATGTCCTTTGTTTTCATTAAATGTCACTTTATTTGGAGAAATAACTTCAACTACTAAATACTATCAGTTTGAAAACATAAGTTATTTCCTTGGATGATGTTTACTAAAGCATGAGTTTTGCTATTCTAAGTTTACAGTGACTTATTTTGCTAAAAAGTTATGTCTTAGTGCAGAATGCTATATACAGTGCTTTTTGCAAGTTTTTCAAGATTTAACTGCATTCTTACAGTTGTAGAGCTTTCTTTTTATCTTTGCGTGGTTAAGAATCATTCATGATATGCTTGGAACCTGTAAATGTGGTTGTTGGCTTTTTTCTGCATCTTCGATTTTGGGAGTACTAGTATGACTGTTTCAGCAATATGCCATTTGAACTTATTTTGTGTATATGTTCTCGTGCACATTAACCTTTCATGCTATATGAGTATTTTGTTTCAATTTTTTATGAACTCTTATTTCTAAATATATTATCATGAACGCATAATTTGAACTATCACTCAATTGTCAAATGATATTTAACATTTGGTTCAAGATACTTTGAGGGACTATTTTGAACACCATGAATATCTCTAATTTTTTTTAATGTCATTTTGGTATGTCTTTTGCATTTAATTAAAGTTCTAATAAGTCTAACACTCACCATTTGCATTTTCTTCCTAATTAACTATTTGGTTTTATAAATGCATTGATTAAGTTAAATTTATCCTATGAGAGACGTTTATGATCAAAACTATCCTTGAAGGCAAAACTTGAGCAATCAGAAGCAAAGACTATCCAGGTGGAATCAATGGTTGAGGAAGAGAGGAGTGCAAGACTTGAAACGAAAGCTGAGCTAGAAAACTTGAGAGGAATGATCCAACAATTCATGTCATCACAAGGAGGAGAAACATCGAAATAG

mRNA sequence

ATGCATTGGTATTTTAGTAGAAACAAAATTAACTACGAACGGTCCTATTGCTTCCGCTGTAACGGAATTGGGGAATTCCAATCCTTCACCACCCTCGTCTTTTTCGCCCCTGCGCACCAAATCTCCCGCACCATCGGTTCTCCTCCGTCGCCGTTGTCATTTATCTCGCGTCGCCGTCGTTGTTTTGCTCGTGCTTCCGTCGTCGTTCCACTCTGTAGTCATCGTCTTTTCTCGCTTCAACAGTTAGAGCTTCCCAAACTGTGGTTTGCCCATATGCGAGAGGTGAAACCTGGTATCTACGTCACCTACAATGGCGACTATTTTGACTGGCCATTCCTTGAAAGTAGAGCAACGTATCATGGATTTAAAATGAGTGATGCAAAACTTGAGCAATCAGAAGCAAAGACTATCCAGGTGGAATCAATGGTTGAGGAAGAGAGGAGTGCAAGACTTGAAACGAAAGCTGAGCTAGAAAACTTGAGAGGAATGATCCAACAATTCATGTCATCACAAGGAGGAGAAACATCGAAATAG

Coding sequence (CDS)

ATGCATTGGTATTTTAGTAGAAACAAAATTAACTACGAACGGTCCTATTGCTTCCGCTGTAACGGAATTGGGGAATTCCAATCCTTCACCACCCTCGTCTTTTTCGCCCCTGCGCACCAAATCTCCCGCACCATCGGTTCTCCTCCGTCGCCGTTGTCATTTATCTCGCGTCGCCGTCGTTGTTTTGCTCGTGCTTCCGTCGTCGTTCCACTCTGTAGTCATCGTCTTTTCTCGCTTCAACAGTTAGAGCTTCCCAAACTGTGGTTTGCCCATATGCGAGAGGTGAAACCTGGTATCTACGTCACCTACAATGGCGACTATTTTGACTGGCCATTCCTTGAAAGTAGAGCAACGTATCATGGATTTAAAATGAGTGATGCAAAACTTGAGCAATCAGAAGCAAAGACTATCCAGGTGGAATCAATGGTTGAGGAAGAGAGGAGTGCAAGACTTGAAACGAAAGCTGAGCTAGAAAACTTGAGAGGAATGATCCAACAATTCATGTCATCACAAGGAGGAGAAACATCGAAATAG

Protein sequence

MHWYFSRNKINYERSYCFRCNGIGEFQSFTTLVFFAPAHQISRTIGSPPSPLSFISRRRRCFARASVVVPLCSHRLFSLQQLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSDAKLEQSEAKTIQVESMVEEERSARLETKAELENLRGMIQQFMSSQGGETSK

Homology

[Close]

BLAST of Lag0022001 vs. NCBI nr
Match: XP_022153062.1 (DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia])

HSP 1 Score: 99.0 bits (245), Expect = 4.4e-17
Identity = 43/46 (93.48%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           +LEL KLWFAHMREVKPGIYVTYNGDYFDWPFLESRA YHGFKMSD
Sbjct: 312 ELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD 357

[Close]

BLAST of Lag0022001 vs. NCBI nr
Match: XP_022153061.1 (DNA polymerase epsilon catalytic subunit A-like isoform X1 [Momordica charantia])

HSP 1 Score: 99.0 bits (245), Expect = 4.4e-17
Identity = 43/46 (93.48%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           +LEL KLWFAHMREVKPGIYVTYNGDYFDWPFLESRA YHGFKMSD
Sbjct: 312 ELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD 357

[Close]

BLAST of Lag0022001 vs. NCBI nr
Match: KAF9684575.1 (hypothetical protein SADUNF_Sadunf04G0132300 [Salix dunnii])

HSP 1 Score: 97.8 bits (242), Expect = 9.8e-17
Identity = 42/46 (91.30%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           ++EL KLWFAHMREVKPGIYVTYNGDYFDWPFLESRA YHGFKMSD
Sbjct: 313 EVELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD 358

[Close]

BLAST of Lag0022001 vs. NCBI nr
Match: KAG5248027.1 (DNA-directed DNA polymerase epsilon catalytic family protein [Salix suchowensis])

HSP 1 Score: 97.8 bits (242), Expect = 9.8e-17
Identity = 42/46 (91.30%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           ++EL KLWFAHMREVKPGIYVTYNGDYFDWPFLESRA YHGFKMSD
Sbjct: 313 EVELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD 358

[Close]

BLAST of Lag0022001 vs. NCBI nr
Match: KAB5561539.1 (hypothetical protein DKX38_006496 [Salix brachista])

HSP 1 Score: 97.8 bits (242), Expect = 9.8e-17
Identity = 42/46 (91.30%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           ++EL KLWFAHMREVKPGIYVTYNGDYFDWPFLESRA YHGFKMSD
Sbjct: 313 EVELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSD 358

[Close]

BLAST of Lag0022001 vs. ExPASy Swiss-Prot
Match: F4IFN6 (DNA polymerase epsilon catalytic subunit B OS=Arabidopsis thaliana OX=3702 GN=POL2B PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.6e-14
Identity = 30/46 (65.22%), Postives = 42/46 (91.30%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           ++EL + WF+HM+E+KPGIYVTYNGD+FDWPF+E RA++HG KM++
Sbjct: 304 EVELLRKWFSHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNE 349

[Close]

BLAST of Lag0022001 vs. ExPASy Swiss-Prot
Match: F4HW04 (DNA polymerase epsilon catalytic subunit A OS=Arabidopsis thaliana OX=3702 GN=POL2A PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-13
Identity = 30/46 (65.22%), Postives = 41/46 (89.13%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           ++EL + WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG KM++
Sbjct: 312 EVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNE 357

[Close]

BLAST of Lag0022001 vs. ExPASy Swiss-Prot
Match: Q9WVF7 (DNA polymerase epsilon catalytic subunit A OS=Mus musculus OX=10090 GN=Pole PE=1 SV=3)

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 25/44 (56.82%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKM 125
           ++ L + WF H++E KP I VTYNGD+FDWPF+E+RA  HG  M
Sbjct: 340 EVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSM 383

[Close]

BLAST of Lag0022001 vs. ExPASy Swiss-Prot
Match: Q07864 (DNA polymerase epsilon catalytic subunit A OS=Homo sapiens OX=9606 GN=POLE PE=1 SV=5)

HSP 1 Score: 63.9 bits (154), Expect = 2.1e-09
Identity = 25/44 (56.82%), Postives = 32/44 (72.73%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKM 125
           +  L + WF H++E KP I VTYNGD+FDWPF+E+RA  HG  M
Sbjct: 340 EAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSM 383

[Close]

BLAST of Lag0022001 vs. ExPASy Swiss-Prot
Match: Q6CUS7 (DNA polymerase epsilon catalytic subunit A OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=POL2 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.9e-08
Identity = 22/46 (47.83%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 81  QLELPKLWFAHMREVKPGIYVTYNGDYFDWPFLESRATYHGFKMSD 127
           ++ L + +F H+R+V+P +  T+NGD+FDWPF+E+RA +HG  M D
Sbjct: 332 EMALLQRFFEHIRDVRPTVISTFNGDFFDWPFVENRAKFHGLNMFD 377

The following BLAST results are available for this feature:

BLAST of Lag0022001 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153062.1	4.4e-17	93.48	DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia]	[more]
XP_022153061.1	4.4e-17	93.48	DNA polymerase epsilon catalytic subunit A-like isoform X1 [Momordica charantia]	[more]
KAF9684575.1	9.8e-17	91.30	hypothetical protein SADUNF_Sadunf04G0132300 [Salix dunnii]	[more]
KAG5248027.1	9.8e-17	91.30	DNA-directed DNA polymerase epsilon catalytic family protein [Salix suchowensis]	[more]
KAB5561539.1	9.8e-17	91.30	hypothetical protein DKX38_006496 [Salix brachista]	[more]

BLAST of Lag0022001 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IFN6	3.6e-14	65.22	DNA polymerase epsilon catalytic subunit B OS=Arabidopsis thaliana OX=3702 GN=PO...	[more]
F4HW04	1.1e-13	65.22	DNA polymerase epsilon catalytic subunit A OS=Arabidopsis thaliana OX=3702 GN=PO...	[more]
Q9WVF7	1.2e-09	56.82	DNA polymerase epsilon catalytic subunit A OS=Mus musculus OX=10090 GN=Pole PE=1...	[more]
Q07864	2.1e-09	56.82	DNA polymerase epsilon catalytic subunit A OS=Homo sapiens OX=9606 GN=POLE PE=1 ...	[more]
Q6CUS7	3.9e-08	47.83	DNA polymerase epsilon catalytic subunit A OS=Kluyveromyces lactis (strain ATCC ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 75..168 e-value: 2.5E-11 score: 45.5
IPR006133	DNA-directed DNA polymerase, family B, exonuclease domain	PFAM	PF03104	DNA_pol_B_exo1	coord: 87..129 e-value: 2.7E-10 score: 39.9
IPR029703	DNA polymerase epsilon catalytic subunit	PANTHER	PTHR10670	DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A	coord: 83..126
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 83..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0022001.1	Lag0022001.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006287	base-excision repair, gap-filling
biological_process	GO:0045004	DNA replication proofreading
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0000278	mitotic cell cycle
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006297	nucleotide-excision repair, DNA gap filling
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0006260	DNA replication
biological_process	GO:0006281	DNA repair
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0008310	single-stranded DNA 3'-5' exodeoxyribonuclease activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003676	nucleic acid binding