Lag0021948 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021948
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr7: 14396564 .. 14397670 (+)
RNA-Seq ExpressionLag0021948
SyntenyLag0021948
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCTCCTTCAATAGAGAAGAGCAATTCTGAGATTGAAATCTCATCTCAGAGTCCGAAAATCATAAACCCAGGCAACAAGATCACTACGATGAAGCTTGACGAAGACAATTTCCTATTGTGGAAGTTGCAGATCCTCACCACTTTGCGAGGACATGGGTTGAAACACCATCTCGATGAAGATGCGGAAGTTCCGTCGAAGTATCTCACAAGTGCAGATTCTTCAAAAATCCCTAATCCGGCGTATGATCATTGGGTTCGACAGGATAGCCTAATTATCGCCTGGTTGTTAGGCTCAATGTCCAACTCTCTTCTTTCGGAAATGCTGGATTGTGAAACGACTCGAGAAGTGTGGAAAATATTGCAAAATCGCTTTTCCTCACGGAATGTTGCTCGAATTATGGACCTCCAGTCGAAATTAGAATCCCTCAAGAAAGGTAACCTGAAACTCGAAGATTACTTTCTCAGAGTCAGAAATCTTGTTGATTTGTTAAATGCTGCTGGAAGGAAAATTTCACATGAAGATCATGTCTTATACATTCTTAAGGGACTAGGAACGAAATATGACTCCACGGTGAATGTAATTACGGAAAAAGACGAGACTCCCCCGTTGCAGAAGGTGTATTCGCTACTGTTTACCCAAGAAAACAGAATAGCTAGGAATCTTTCCATCAATCCTGATGGTTCAACTCCTTCTGTCAACCTTACAACTCAAGCTGGACCGAAATAACAACAGACTGTTTCATCTGACTCCAATAATCACAGGAACTCAAATGGAAAGGGTAACAACAATCGTCGATCTTGGAACAATAACAATAAACCACAGTGTCAGTTGTGCAGACGATTTGGACATACGGTTCAAAGGTGTTATTATCGGTTCGAACGTTGGTTTCAAGGACCAAATGGTAATTCAAACAATCAACAAGTATCACCTGGCCTTGGACAACAATCCTCTGGTTCTAATAACCAGCAAGCAGCCATGAATGCCTTCGTAGTGCAAAATGATCTTAACAAAGACAATCACTGGTATCCGGATTCAGGAGCATCGAATCACGTCACCAATGTTGGGATTGGTGCCCTAAAACTCGTAGATAATGAATGTTAA

mRNA sequence

ATGGAGTCTCCTTCAATAGAGAAGAGCAATTCTGAGATTGAAATCTCATCTCAGAGTCCGAAAATCATAAACCCAGGCAACAAGATCACTACGATGAAGCTTGACGAAGACAATTTCCTATTGTGGAAGTTGCAGATCCTCACCACTTTGCGAGGACATGGGTTGAAACACCATCTCGATGAAGATGCGGAAGTTCCGTCGAAGTATCTCACAAGTGCAGATTCTTCAAAAATCCCTAATCCGGCGTATGATCATTGGGTTCGACAGGATAGCCTAATTATCGCCTGGTTGTTAGGCTCAATGTCCAACTCTCTTCTTTCGGAAATGCTGGATTGTGAAACGACTCGAGAAGTGTGGAAAATATTGCAAAATCGCTTTTCCTCACGGAATGTTGCTCGAATTATGGACCTCCAGTCGAAATTAGAATCCCTCAAGAAAGGTAACCTGAAACTCGAAGATTACTTTCTCAGAGTCAGAAATCTTGTTGATTTGTTAAATGCTGCTGGAAGGAAAATTTCACATGAAGATCATGTCTTATACATTCTTAAGGGACTAGGAACGAAATATGACTCCACGGTGAATGTAATTACGGAAAAAGACGAGACTCCCCCGTTGCAGAAGACTGTTTCATCTGACTCCAATAATCACAGGAACTCAAATGGAAAGGGTAACAACAATCGTCGATCTTGGAACAATAACAATAAACCACAGTGTCAGTTGTGCAGACGATTTGGACATACGGTTCAAAGGTGTTATTATCGGTTCGAACGTTGGTTTCAAGGACCAAATGGTAATTCAAACAATCAACAAGTATCACCTGGCCTTGGACAACAATCCTCTGGTTCTAATAACCAGCAAGCAGCCATGAATGCCTTCGTAGTGCAAAATGATCTTAACAAAGACAATCACTGGTATCCGGATTCAGGAGCATCGAATCACGTCACCAATGTTGGGATTGGTGCCCTAAAACTCGTAGATAATGAATGTTAA

Coding sequence (CDS)

ATGGAGTCTCCTTCAATAGAGAAGAGCAATTCTGAGATTGAAATCTCATCTCAGAGTCCGAAAATCATAAACCCAGGCAACAAGATCACTACGATGAAGCTTGACGAAGACAATTTCCTATTGTGGAAGTTGCAGATCCTCACCACTTTGCGAGGACATGGGTTGAAACACCATCTCGATGAAGATGCGGAAGTTCCGTCGAAGTATCTCACAAGTGCAGATTCTTCAAAAATCCCTAATCCGGCGTATGATCATTGGGTTCGACAGGATAGCCTAATTATCGCCTGGTTGTTAGGCTCAATGTCCAACTCTCTTCTTTCGGAAATGCTGGATTGTGAAACGACTCGAGAAGTGTGGAAAATATTGCAAAATCGCTTTTCCTCACGGAATGTTGCTCGAATTATGGACCTCCAGTCGAAATTAGAATCCCTCAAGAAAGGTAACCTGAAACTCGAAGATTACTTTCTCAGAGTCAGAAATCTTGTTGATTTGTTAAATGCTGCTGGAAGGAAAATTTCACATGAAGATCATGTCTTATACATTCTTAAGGGACTAGGAACGAAATATGACTCCACGGTGAATGTAATTACGGAAAAAGACGAGACTCCCCCGTTGCAGAAGACTGTTTCATCTGACTCCAATAATCACAGGAACTCAAATGGAAAGGGTAACAACAATCGTCGATCTTGGAACAATAACAATAAACCACAGTGTCAGTTGTGCAGACGATTTGGACATACGGTTCAAAGGTGTTATTATCGGTTCGAACGTTGGTTTCAAGGACCAAATGGTAATTCAAACAATCAACAAGTATCACCTGGCCTTGGACAACAATCCTCTGGTTCTAATAACCAGCAAGCAGCCATGAATGCCTTCGTAGTGCAAAATGATCTTAACAAAGACAATCACTGGTATCCGGATTCAGGAGCATCGAATCACGTCACCAATGTTGGGATTGGTGCCCTAAAACTCGTAGATAATGAATGTTAA

Protein sequence

MESPSIEKSNSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKYLTSADSSKIPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTVNVITEKDETPPLQKTVSSDSNNHRNSNGKGNNNRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSNNQQAAMNAFVVQNDLNKDNHWYPDSGASNHVTNVGIGALKLVDNEC
Homology
BLAST of Lag0021948 vs. NCBI nr
Match: XP_022154487.1 (uncharacterized protein LOC111021757 [Momordica charantia])

HSP 1 Score: 270.8 bits (691), Expect = 1.6e-68
Identity = 154/377 (40.85%), Postives = 220/377 (58.36%), Query Frame = 0

Query: 10  NSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKY 69
           NS+     Q+ K INPG+K++ ++L++DN LLWK QI T L+G+GL+ ++D + + P+++
Sbjct: 9   NSDAARIIQASKTINPGSKVSIVRLNDDNXLLWKFQIRTALQGNGLESYIDSNEDTPAQF 68

Query: 70  L-----TSADSSKIPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQN 129
           +      S+ SS   NPAY  W++QD LI AWLLGSM+  +LS+MLDC++ RE+W +L+ 
Sbjct: 69  VQTTEDESSSSSLQQNPAYFEWIKQDKLISAWLLGSMNEDILSQMLDCKSAREIWTVLEC 128

Query: 130 RFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKG 189
            F+SR +AR+M L+ KLE+ KKGNL L+DYFL+++NLVD L  AG+K+S EDH+++IL G
Sbjct: 129 MFASRTLARVMQLKLKLENFKKGNLSLKDYFLKIKNLVDSLAIAGKKLSTEDHIMHILAG 188

Query: 190 LGTKYDSTVNVITEKDETPPLQKTVS----SDSNNHRN---------------------- 249
           LG ++D+ ++VIT ++    LQ+  S     +  N RN                      
Sbjct: 189 LGPEFDAIISVITARNMPQTLQEVCSLLLQQEGRNERNLINSDGSLPSVNLTLNDSSKKN 248

Query: 250 ----------------SNGKGNN----NRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERW 309
                             G+G N    NRR+W  NNKPQCQ+C RFGHT  RCY RFER 
Sbjct: 249 NLHQSKCFNPHQSNYSQRGRGTNNRSSNRRNWTGNNKPQCQICGRFGHTALRCYMRFERN 308

Query: 310 FQGPNGN------------------SNNQQVSPGLGQQSSGSNN-QQAAMNAFVVQNDLN 317
           F GPN N                  S+N   SP     +   N+   + M A +V  D N
Sbjct: 309 FLGPNMNPNTFSPAGFSSGFPLNTPSHNAFSSPATQNGNFSMNSASPSQMQALMVAQDFN 368

BLAST of Lag0021948 vs. NCBI nr
Match: KAA0048297.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 240.4 bits (612), Expect = 2.3e-59
Identity = 141/376 (37.50%), Postives = 207/376 (55.05%), Query Frame = 0

Query: 1   MESPSIEKSNSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLD 60
           M S S        E SS   +I   GNKI+ +KL++D FLLWK QILT L  + L++ L+
Sbjct: 1   MSSTSSLLGVENTEASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLE 60

Query: 61  EDAEVPSKYLTSADSSK-----IPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETT 120
            ++E PSKYL S +SS       PNPAY  W RQD LI +WLLGSMS  +L++ML C++ 
Sbjct: 61  SESEPPSKYLISTESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSA 120

Query: 121 REVWKILQNRFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHE 180
           +E+W+ LQ  FSSR +A+ M  ++KL ++KKG++ L++YFL++   VD L +  + +S +
Sbjct: 121 KEIWETLQGIFSSRYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSD 180

Query: 181 DHVLYILKGLGTKYDSTVNVITEKDETPPLQKTVS------------------------- 240
           DH+LYIL GLG+ Y S ++VI+ + ++P +Q+ +S                         
Sbjct: 181 DHILYILAGLGSDYQSMISVISARTDSPSVQEVMSLLLTQESQNESKLISETALPSVNIV 240

Query: 241 ---------------------SDSNNHRNSNGKGNNNRRSWNNNNKPQCQLCRRFGHTVQ 300
                                + S N R   G G +NR    N NKPQCQ+C + G++  
Sbjct: 241 TQTTEKGAESYIRTNQNNYHNNHSYNQRGGRGNGRSNRGRRGNRNKPQCQICAKLGYSAD 300

Query: 301 RCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSNNQQAAMNAFVVQNDLNKDNHWYPDSG 322
           RC++R+          SN+   SP     S  + N    M+A V   DLN D++WYPDSG
Sbjct: 301 RCFFRY-------TPRSNSSGYSPNSHNTSYTNMNNHPQMSAMVAALDLNIDSNWYPDSG 360

BLAST of Lag0021948 vs. NCBI nr
Match: TYK10642.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 240.4 bits (612), Expect = 2.3e-59
Identity = 141/376 (37.50%), Postives = 207/376 (55.05%), Query Frame = 0

Query: 1   MESPSIEKSNSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLD 60
           M S S        E SS   +I   GNKI+ +KL++D FLLWK QILT L  + L++ L+
Sbjct: 1   MSSTSSLLGVENTEASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLE 60

Query: 61  EDAEVPSKYLTSADSSK-----IPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETT 120
            ++E PSKYL S +SS       PNPAY  W RQD LI +WLLGSMS  +L++ML C++ 
Sbjct: 61  SESEPPSKYLISTESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSA 120

Query: 121 REVWKILQNRFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHE 180
           +E+W+ LQ  FSSR +A+ M  ++KL ++KKG++ L++YFL++   VD L +  + +S +
Sbjct: 121 KEIWETLQGIFSSRYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSD 180

Query: 181 DHVLYILKGLGTKYDSTVNVITEKDETPPLQKTVS------------------------- 240
           DH+LYIL GLG+ Y S ++VI+ + ++P +Q+ +S                         
Sbjct: 181 DHILYILAGLGSDYQSMISVISARTDSPSVQEVMSLLLTQESQNESKLISETALPSVNIV 240

Query: 241 ---------------------SDSNNHRNSNGKGNNNRRSWNNNNKPQCQLCRRFGHTVQ 300
                                + S N R   G G +NR    N NKPQCQ+C + G++  
Sbjct: 241 TQTTEKGAESYIRTNQNNYHNNHSYNQRGGRGNGRSNRGRRGNRNKPQCQICAKLGYSAD 300

Query: 301 RCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSNNQQAAMNAFVVQNDLNKDNHWYPDSG 322
           RC++R+          SN+   SP     S  + N    M+A V   DLN D++WYPDSG
Sbjct: 301 RCFFRY-------TPRSNSSGYSPNSHNTSYTNMNNHPQMSAMVAALDLNIDSNWYPDSG 360

BLAST of Lag0021948 vs. NCBI nr
Match: XP_022136882.1 (dr1-associated corepressor homolog isoform X1 [Momordica charantia])

HSP 1 Score: 212.6 bits (540), Expect = 5.1e-51
Identity = 124/289 (42.91%), Postives = 171/289 (59.17%), Query Frame = 0

Query: 87  VRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQSKLESLKK 146
           ++QD LI +WL  SM   +L EM+ C T REVW+IL+N ++SRN+AR+M L+SKLE++KK
Sbjct: 1   MKQDKLITSWLFSSMFEEILGEMIHCNTAREVWQILENLYTSRNLARVMQLKSKLENIKK 60

Query: 147 GNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTVNVITEKDETPPLQ 206
           GNL L+DYF +V+ LVD L AAG+K++ EDH+++IL GL ++++STV+VI+ + +T  LQ
Sbjct: 61  GNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISARTQTQTLQ 120

Query: 207 KTVS-----------------------------SDSN-------------NHRNSNGKGN 266
           +  S                              +SN             N+R+ N    
Sbjct: 121 EVYSLLLSHEGRNERNSINTDGTLPSVNLTQQTKNSNSAQSIDGQRPYMQNNRSKNSGNP 180

Query: 267 NNRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSN- 317
           N RR+WN+NN+PQCQ+  +FGHT  RCY RFE+ F GPNG S+ Q    G    S  SN 
Sbjct: 181 NFRRNWNSNNRPQCQIYGKFGHTALRCYLRFEKTFLGPNGQSHMQHKFSGGSNSSINSNM 240

BLAST of Lag0021948 vs. NCBI nr
Match: XP_022136883.1 (dr1-associated corepressor homolog isoform X2 [Momordica charantia])

HSP 1 Score: 212.6 bits (540), Expect = 5.1e-51
Identity = 124/289 (42.91%), Postives = 171/289 (59.17%), Query Frame = 0

Query: 87  VRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQSKLESLKK 146
           ++QD LI +WL  SM   +L EM+ C T REVW+IL+N ++SRN+AR+M L+SKLE++KK
Sbjct: 1   MKQDKLITSWLFSSMFEEILGEMIHCNTAREVWQILENLYTSRNLARVMQLKSKLENIKK 60

Query: 147 GNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTVNVITEKDETPPLQ 206
           GNL L+DYF +V+ LVD L AAG+K++ EDH+++IL GL ++++STV+VI+ + +T  LQ
Sbjct: 61  GNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISARTQTQTLQ 120

Query: 207 KTVS-----------------------------SDSN-------------NHRNSNGKGN 266
           +  S                              +SN             N+R+ N    
Sbjct: 121 EVYSLLLSHEGRNERNSINTDGTLPSVNLTQQTKNSNSAQSIDGQRPYMQNNRSKNSGNP 180

Query: 267 NNRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSN- 317
           N RR+WN+NN+PQCQ+  +FGHT  RCY RFE+ F GPNG S+ Q    G    S  SN 
Sbjct: 181 NFRRNWNSNNRPQCQIYGKFGHTALRCYLRFEKTFLGPNGQSHMQHKFSGGSNSSINSNM 240

BLAST of Lag0021948 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 6.9e-19
Identity = 84/353 (23.80%), Postives = 146/353 (41.36%), Query Frame = 0

Query: 14  EISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKYLTSA 73
           E+   +  I+N  N     KL   N+L+W  Q+     G+ L   LD    +P   +   
Sbjct: 7   ELVLNNTSILNV-NMSNVTKLTSTNYLMWSRQVHALFDGYELAGFLDGSTTMPPATI-GT 66

Query: 74  DSSKIPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVAR 133
           D++   NP Y  W RQD LI + +LG++S S+   +    T  ++W+ L+  +++ +   
Sbjct: 67  DAAPRVNPDYTRWKRQDKLIYSAVLGAISMSVQPAVSRATTAAQIWETLRKIYANPSYGH 126

Query: 134 IMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTV 193
           +  L+++L+   KG   ++DY   +    D L   G+ + H++ V  +L+ L  +Y   +
Sbjct: 127 VTQLRTQLKQWTKGTKTIDDYMQGLVTRFDQLALLGKPMDHDEQVERVLENLPEEYKPVI 186

Query: 194 NVITEKDETPPL--------------------------------QKTVSSDSNNHRNSNG 253
           + I  KD  P L                                + T ++++NN+ N N 
Sbjct: 187 DQIAAKDTPPTLTEIHERLLNHESKILAVSSATVIPITANAVSHRNTTTTNNNNNGNRNN 246

Query: 254 K-----GNNNRRSW----------NNNNKP---QCQLCRRFGHTVQRCYYRFERWFQGPN 313
           +      NNN + W          NN +KP   +CQ+C   GH+ +RC            
Sbjct: 247 RYDNRNNNNNSKPWQQSSTNFHPNNNQSKPYLGKCQICGVQGHSAKRC------------ 306

Query: 314 GNSNNQQVSPGLGQQSSGSNNQQAAMNAFVVQNDLNKDNHWYPDSGASNHVTN 317
             S  Q     +  Q   S        A +        N+W  DSGA++H+T+
Sbjct: 307 --SQLQHFLSSVNSQQPPSPFTPWQPRANLALGSPYSSNNWLLDSGATHHITS 343

BLAST of Lag0021948 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-13
Identity = 86/360 (23.89%), Postives = 140/360 (38.89%), Query Frame = 0

Query: 14  EISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKYLTSA 73
           EI   +  I+N  N     KL   N+L+W  Q+     G+ L   LD    +P   +   
Sbjct: 7   EIVLVNTNILNV-NMSNVTKLTSTNYLMWSRQVHALFDGYELAGFLDGSTPMPPATI-GT 66

Query: 74  DSSKIPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVAR 133
           D+    NP Y  W RQD LI + +LG++S S+   +    T  ++W+ L+  +++ +   
Sbjct: 67  DAVPRVNPDYTRWRRQDKLIYSAILGAISMSVQPAVSRATTAAQIWETLRKIYANPSYGH 126

Query: 134 IMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTV 193
           +                     LR     D L   G+ + H++ V  +L+ L   Y   +
Sbjct: 127 VTQ-------------------LRFITRFDQLALLGKPMDHDEQVERVLENLPDDYKPVI 186

Query: 194 NVITEKDETPPL----QKTVSSDS-----------------------NNHRNSNGKGNNN 253
           + I  KD  P L    ++ ++ +S                       N +RN N +G+N 
Sbjct: 187 DQIAAKDTPPSLTEIHERLINRESKLLALNSAEVVPITANVVTHRNTNTNRNQNNRGDN- 246

Query: 254 RRSWNNNN--------------------KP---QCQLCRRFGHTVQRCYYRFERWFQGPN 313
            R++NNNN                    KP   +CQ+C   GH+ +RC            
Sbjct: 247 -RNYNNNNNRSNSWQPSSSGSRSDNRQPKPYLGRCQICSVQGHSAKRC------------ 306

Query: 314 GNSNNQQVSPGLGQQSSGSNNQQAAM-------NAFVVQNDLNKDNHWYPDSGASNHVTN 317
                    P L Q  S +N QQ+          A +  N     N+W  DSGA++H+T+
Sbjct: 307 ---------PQLHQFQSTTNQQQSTSPFTPWQPRANLAVNSPYNANNWLLDSGATHHITS 322

BLAST of Lag0021948 vs. ExPASy TrEMBL
Match: A0A6J1DLT9 (uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021757 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 7.6e-69
Identity = 154/377 (40.85%), Postives = 220/377 (58.36%), Query Frame = 0

Query: 10  NSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKY 69
           NS+     Q+ K INPG+K++ ++L++DN LLWK QI T L+G+GL+ ++D + + P+++
Sbjct: 9   NSDAARIIQASKTINPGSKVSIVRLNDDNXLLWKFQIRTALQGNGLESYIDSNEDTPAQF 68

Query: 70  L-----TSADSSKIPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQN 129
           +      S+ SS   NPAY  W++QD LI AWLLGSM+  +LS+MLDC++ RE+W +L+ 
Sbjct: 69  VQTTEDESSSSSLQQNPAYFEWIKQDKLISAWLLGSMNEDILSQMLDCKSAREIWTVLEC 128

Query: 130 RFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKG 189
            F+SR +AR+M L+ KLE+ KKGNL L+DYFL+++NLVD L  AG+K+S EDH+++IL G
Sbjct: 129 MFASRTLARVMQLKLKLENFKKGNLSLKDYFLKIKNLVDSLAIAGKKLSTEDHIMHILAG 188

Query: 190 LGTKYDSTVNVITEKDETPPLQKTVS----SDSNNHRN---------------------- 249
           LG ++D+ ++VIT ++    LQ+  S     +  N RN                      
Sbjct: 189 LGPEFDAIISVITARNMPQTLQEVCSLLLQQEGRNERNLINSDGSLPSVNLTLNDSSKKN 248

Query: 250 ----------------SNGKGNN----NRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERW 309
                             G+G N    NRR+W  NNKPQCQ+C RFGHT  RCY RFER 
Sbjct: 249 NLHQSKCFNPHQSNYSQRGRGTNNRSSNRRNWTGNNKPQCQICGRFGHTALRCYMRFERN 308

Query: 310 FQGPNGN------------------SNNQQVSPGLGQQSSGSNN-QQAAMNAFVVQNDLN 317
           F GPN N                  S+N   SP     +   N+   + M A +V  D N
Sbjct: 309 FLGPNMNPNTFSPAGFSSGFPLNTPSHNAFSSPATQNGNFSMNSASPSQMQALMVAQDFN 368

BLAST of Lag0021948 vs. ExPASy TrEMBL
Match: A0A5A7U233 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00060 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.1e-59
Identity = 141/376 (37.50%), Postives = 207/376 (55.05%), Query Frame = 0

Query: 1   MESPSIEKSNSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLD 60
           M S S        E SS   +I   GNKI+ +KL++D FLLWK QILT L  + L++ L+
Sbjct: 1   MSSTSSLLGVENTEASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLE 60

Query: 61  EDAEVPSKYLTSADSSK-----IPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETT 120
            ++E PSKYL S +SS       PNPAY  W RQD LI +WLLGSMS  +L++ML C++ 
Sbjct: 61  SESEPPSKYLISTESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSA 120

Query: 121 REVWKILQNRFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHE 180
           +E+W+ LQ  FSSR +A+ M  ++KL ++KKG++ L++YFL++   VD L +  + +S +
Sbjct: 121 KEIWETLQGIFSSRYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSD 180

Query: 181 DHVLYILKGLGTKYDSTVNVITEKDETPPLQKTVS------------------------- 240
           DH+LYIL GLG+ Y S ++VI+ + ++P +Q+ +S                         
Sbjct: 181 DHILYILAGLGSDYQSMISVISARTDSPSVQEVMSLLLTQESQNESKLISETALPSVNIV 240

Query: 241 ---------------------SDSNNHRNSNGKGNNNRRSWNNNNKPQCQLCRRFGHTVQ 300
                                + S N R   G G +NR    N NKPQCQ+C + G++  
Sbjct: 241 TQTTEKGAESYIRTNQNNYHNNHSYNQRGGRGNGRSNRGRRGNRNKPQCQICAKLGYSAD 300

Query: 301 RCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSNNQQAAMNAFVVQNDLNKDNHWYPDSG 322
           RC++R+          SN+   SP     S  + N    M+A V   DLN D++WYPDSG
Sbjct: 301 RCFFRY-------TPRSNSSGYSPNSHNTSYTNMNNHPQMSAMVAALDLNIDSNWYPDSG 360

BLAST of Lag0021948 vs. ExPASy TrEMBL
Match: A0A5D3CH97 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00040 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.1e-59
Identity = 141/376 (37.50%), Postives = 207/376 (55.05%), Query Frame = 0

Query: 1   MESPSIEKSNSEIEISSQSPKIINPGNKITTMKLDEDNFLLWKLQILTTLRGHGLKHHLD 60
           M S S        E SS   +I   GNKI+ +KL++D FLLWK QILT L  + L++ L+
Sbjct: 1   MSSTSSLLGVENTEASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLE 60

Query: 61  EDAEVPSKYLTSADSSK-----IPNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETT 120
            ++E PSKYL S +SS       PNPAY  W RQD LI +WLLGSMS  +L++ML C++ 
Sbjct: 61  SESEPPSKYLISTESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSA 120

Query: 121 REVWKILQNRFSSRNVARIMDLQSKLESLKKGNLKLEDYFLRVRNLVDLLNAAGRKISHE 180
           +E+W+ LQ  FSSR +A+ M  ++KL ++KKG++ L++YFL++   VD L +  + +S +
Sbjct: 121 KEIWETLQGIFSSRYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSD 180

Query: 181 DHVLYILKGLGTKYDSTVNVITEKDETPPLQKTVS------------------------- 240
           DH+LYIL GLG+ Y S ++VI+ + ++P +Q+ +S                         
Sbjct: 181 DHILYILAGLGSDYQSMISVISARTDSPSVQEVMSLLLTQESQNESKLISETALPSVNIV 240

Query: 241 ---------------------SDSNNHRNSNGKGNNNRRSWNNNNKPQCQLCRRFGHTVQ 300
                                + S N R   G G +NR    N NKPQCQ+C + G++  
Sbjct: 241 TQTTEKGAESYIRTNQNNYHNNHSYNQRGGRGNGRSNRGRRGNRNKPQCQICAKLGYSAD 300

Query: 301 RCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSNNQQAAMNAFVVQNDLNKDNHWYPDSG 322
           RC++R+          SN+   SP     S  + N    M+A V   DLN D++WYPDSG
Sbjct: 301 RCFFRY-------TPRSNSSGYSPNSHNTSYTNMNNHPQMSAMVAALDLNIDSNWYPDSG 360

BLAST of Lag0021948 vs. ExPASy TrEMBL
Match: A0A6J1C8R2 (dr1-associated corepressor homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008464 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 2.5e-51
Identity = 124/289 (42.91%), Postives = 171/289 (59.17%), Query Frame = 0

Query: 87  VRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQSKLESLKK 146
           ++QD LI +WL  SM   +L EM+ C T REVW+IL+N ++SRN+AR+M L+SKLE++KK
Sbjct: 1   MKQDKLITSWLFSSMFEEILGEMIHCNTAREVWQILENLYTSRNLARVMQLKSKLENIKK 60

Query: 147 GNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTVNVITEKDETPPLQ 206
           GNL L+DYF +V+ LVD L AAG+K++ EDH+++IL GL ++++STV+VI+ + +T  LQ
Sbjct: 61  GNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISARTQTQTLQ 120

Query: 207 KTVS-----------------------------SDSN-------------NHRNSNGKGN 266
           +  S                              +SN             N+R+ N    
Sbjct: 121 EVYSLLLSHEGRNERNSINTDGTLPSVNLTQQTKNSNSAQSIDGQRPYMQNNRSKNSGNP 180

Query: 267 NNRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSN- 317
           N RR+WN+NN+PQCQ+  +FGHT  RCY RFE+ F GPNG S+ Q    G    S  SN 
Sbjct: 181 NFRRNWNSNNRPQCQIYGKFGHTALRCYLRFEKTFLGPNGQSHMQHKFSGGSNSSINSNM 240

BLAST of Lag0021948 vs. ExPASy TrEMBL
Match: A0A6J1C6N9 (dr1-associated corepressor homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008464 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 2.5e-51
Identity = 124/289 (42.91%), Postives = 171/289 (59.17%), Query Frame = 0

Query: 87  VRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQSKLESLKK 146
           ++QD LI +WL  SM   +L EM+ C T REVW+IL+N ++SRN+AR+M L+SKLE++KK
Sbjct: 1   MKQDKLITSWLFSSMFEEILGEMIHCNTAREVWQILENLYTSRNLARVMQLKSKLENIKK 60

Query: 147 GNLKLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTVNVITEKDETPPLQ 206
           GNL L+DYF +V+ LVD L AAG+K++ EDH+++IL GL ++++STV+VI+ + +T  LQ
Sbjct: 61  GNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISARTQTQTLQ 120

Query: 207 KTVS-----------------------------SDSN-------------NHRNSNGKGN 266
           +  S                              +SN             N+R+ N    
Sbjct: 121 EVYSLLLSHEGRNERNSINTDGTLPSVNLTQQTKNSNSAQSIDGQRPYMQNNRSKNSGNP 180

Query: 267 NNRRSWNNNNKPQCQLCRRFGHTVQRCYYRFERWFQGPNGNSNNQQVSPGLGQQSSGSN- 317
           N RR+WN+NN+PQCQ+  +FGHT  RCY RFE+ F GPNG S+ Q    G    S  SN 
Sbjct: 181 NFRRNWNSNNRPQCQIYGKFGHTALRCYLRFEKTFLGPNGQSHMQHKFSGGSNSSINSNM 240

BLAST of Lag0021948 vs. TAIR 10
Match: AT1G34070.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G48050.1); Has 648 Blast hits to 647 proteins in 29 species: Archae - 0; Bacteria - 0; Metazoa - 16; Fungi - 25; Plants - 607; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-13
Identity = 41/174 (23.56%), Postives = 86/174 (49.43%), Query Frame = 0

Query: 32  MKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKYLTSADSSKIPNPAYD-HWVRQD 91
           + ++E N+  W+   LT      +  H+              D + +P  A D +W ++D
Sbjct: 24  LDIEESNYDAWRELFLTHCLSFDVMGHI--------------DGTLLPTNANDVNWQKRD 83

Query: 92  SLIIAWLLGSMS-NSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQSKLESLKKGNL 151
            ++   L G+++        +   T+R++W  ++N+F +   AR + L S+L +   G++
Sbjct: 84  GIVKLSLYGTLTPKQFQGSFVTSSTSRDIWLRIKNQFRNNKDARALRLDSELRTKDIGDM 143

Query: 152 KLEDYFLRVRNLVDLLNAAGRKISHEDHVLYILKGLGTKYDSTVNVITEKDETP 204
           ++ DY+ +++ L D L      ++  + V+Y+L GL  K+D+ +NVI  +   P
Sbjct: 144 RVADYYRKMKKLADSLRNVDVPVTDRNLVMYVLNGLNPKFDNIINVIKHRQPFP 183

BLAST of Lag0021948 vs. TAIR 10
Match: AT1G21280.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Has 707 Blast hits to 705 proteins in 25 species: Archae - 0; Bacteria - 0; Metazoa - 4; Fungi - 0; Plants - 703; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 63.5 bits (153), Expect = 3.5e-10
Identity = 36/137 (26.28%), Postives = 70/137 (51.09%), Query Frame = 0

Query: 20  PKIINPGN-KITTMKLDEDNFLLWKLQILTTLRGHGLKHHLDEDAEVPSKYLTSADSSKI 79
           P I +P +  I  +  DEDN++ WK++  + LR       +D     P  +         
Sbjct: 22  PDIHHPSDFSIQKLSKDEDNYVAWKIRFRSFLRVTKKFGFIDGTLPKPDPF--------- 81

Query: 80  PNPAYDHWVRQDSLIIAWLLGSMSNSLLSEMLDCETTREVWKILQNRFSSRNVARIMDLQ 139
            +P Y  W + +++++ WL+ SM++ LL  ++  ET  ++W+ L+  F      +I  L+
Sbjct: 82  -SPLYQPWEQCNAMVMYWLMNSMTDKLLESVMYAETAHKMWEDLRRVFVPCVDLKIYQLR 141

Query: 140 SKLESLKKGNLKLEDYF 156
            +L +L++G   +E+YF
Sbjct: 142 RRLATLRQGGDSVEEYF 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154487.11.6e-6840.85uncharacterized protein LOC111021757 [Momordica charantia][more]
KAA0048297.12.3e-5937.50Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
TYK10642.12.3e-5937.50Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
XP_022136882.15.1e-5142.91dr1-associated corepressor homolog isoform X1 [Momordica charantia][more]
XP_022136883.15.1e-5142.91dr1-associated corepressor homolog isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q94HW26.9e-1923.80Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT941.1e-1323.89Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1DLT97.6e-6940.85uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A5A7U2331.1e-5937.50Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5D3CH971.1e-5937.50Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A6J1C8R22.5e-5142.91dr1-associated corepressor homolog isoform X2 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1C6N92.5e-5142.91dr1-associated corepressor homolog isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
AT1G34070.11.5e-1323.56CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE... [more]
AT1G21280.13.5e-1026.28CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Ha... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 86..208
e-value: 2.0E-20
score: 72.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 197..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 264..284
NoneNo IPR availablePANTHERPTHR34222:SF48SUBFAMILY NOT NAMEDcoord: 30..317
NoneNo IPR availablePANTHERPTHR34222FAMILY NOT NAMEDcoord: 30..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021948.1Lag0021948.1mRNA