Lag0021897 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0021897
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr7: 13668095 .. 13668600 (+)
RNA-Seq Expression	Lag0021897
Synteny	Lag0021897

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTGAACAACGTTCTTGGTGTGTTGGATATTGCGAAGATATCTCCGAATAGTGTTGTGGAGCTTTGCCACAACTATAAGATTGGTCAGGTAATATTATTCAAAGGAGACGAGACATTTTCAATGTCTTTAGGGGCTCGGGAATCGGTCTCTCCTTCGACATATCCATGGAGATAGCCACCAACACTTATGCATTGCAAAATTGGTTCAACGTTCTTCTGAAGCCTTACATCAACGAAATTAACTTCAACTACATCATTTTCGTTGGGATGGATATCAACGACGAGCTACTACCGACCATGCATGCGGTGCAAGATCTTCTGAACGCCCACTATTTGGTACAAATTATGGTGACTACTATGATAAGTTACCATTCTTCTTTGCTCATTAAAGATCTTCCATCGAGGGGTACTTTGTACCCTAACCTCGCCAAACATCTCAAGCCCGTCATCAATTTTTTAGCTCATCAAGGATCTGTTGAGGATCCCACATTGAAAAGATAA

mRNA sequence

ATGGCATTGAACAACGTTCTTGGTGTGTTGGATATTGCGAAGATATCTCCGAATAGTGTTGTGGAGCTTTGCCACAACTATAAGATTGGTCAGCCTTACATCAACGAAATTAACTTCAACTACATCATTTTCGTTGGGATGGATATCAACGACGAGCTACTACCGACCATGCATGCGGTGCAAGATCTTCTGAACGCCCACTATTTGGTACAAATTATGGTGACTACTATGATAAGTTACCATTCTTCTTTGCTCATTAAAGATCTTCCATCGAGGGGTACTTTGTACCCTAACCTCGCCAAACATCTCAAGCCCGTCATCAATTTTTTAGCTCATCAAGGATCTGTTGAGGATCCCACATTGAAAAGATAA

Coding sequence (CDS)

ATGGCATTGAACAACGTTCTTGGTGTGTTGGATATTGCGAAGATATCTCCGAATAGTGTTGTGGAGCTTTGCCACAACTATAAGATTGGTCAGCCTTACATCAACGAAATTAACTTCAACTACATCATTTTCGTTGGGATGGATATCAACGACGAGCTACTACCGACCATGCATGCGGTGCAAGATCTTCTGAACGCCCACTATTTGGTACAAATTATGGTGACTACTATGATAAGTTACCATTCTTCTTTGCTCATTAAAGATCTTCCATCGAGGGGTACTTTGTACCCTAACCTCGCCAAACATCTCAAGCCCGTCATCAATTTTTTAGCTCATCAAGGATCTGTTGAGGATCCCACATTGAAAAGATAA

Protein sequence

MALNNVLGVLDIAKISPNSVVELCHNYKIGQPYINEINFNYIIFVGMDINDELLPTMHAVQDLLNAHYLVQIMVTTMISYHSSLLIKDLPSRGTLYPNLAKHLKPVINFLAHQGSVEDPTLKR

Homology

[Close]

BLAST of Lag0021897 vs. NCBI nr
Match: XP_022136187.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia])

HSP 1 Score: 76.3 bits (186), Expect = 2.1e-10
Identity = 40/105 (38.10%), Postives = 66/105 (62.86%), Query Frame = 0


Query: 14  KISPNSVVELCHNYKIGQPYINEINFNYIIFVGMDI---NDELLPTMHAVQDLLNAHYLV 73
           K + ++VVE  + Y   +PYI +   NYII     I   +D +LP M ++Q+LLNA YL 
Sbjct: 87  KANESAVVEWFNTYV--EPYIGDFVINYIIVGNKAIPGLDDCILPVMKSLQNLLNARYLG 146

Query: 74  QIMVTTMISYHSSLLIKDLPSRGTLYPNLAKHLKPVINFLAHQGS 116
           Q+ +TT++ Y+++L +KD PS G   PN + +++ ++ FL  +GS
Sbjct: 147 QVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGS 189

[Close]

BLAST of Lag0021897 vs. NCBI nr
Match: XP_038880473.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 74.3 bits (181), Expect = 8.1e-10
Identity = 34/90 (37.78%), Postives = 61/90 (67.78%), Query Frame = 0


Query: 31  QPYINEINFNYII-----FVGMDINDELLPTMHAVQDLLNAHYLVQIMVTTMISYHSSLL 90
           +P++NE   NY+I       G+DI   +LP M + QDLLNA+Y+ Q+ +TT++ Y+++L 
Sbjct: 105 KPFVNEFKINYVIVGDKAIPGLDIC--ILPVMMSFQDLLNANYIGQVKLTTLVGYNAALA 164

Query: 91  IKDLPSRGTLYPNLAKHLKPVINFLAHQGS 116
            KD PS G  +P ++++++ ++ FL+ +GS
Sbjct: 165 YKDPPSSGAFHPTISENMRGILKFLSEEGS 192

[Close]

BLAST of Lag0021897 vs. NCBI nr
Match: KAG7011054.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 71.6 bits (174), Expect = 5.2e-09
Identity = 38/101 (37.62%), Postives = 65/101 (64.36%), Query Frame = 0


Query: 19  SVVELCHNYKIGQPYINEINFNYIIFVG----MDINDELLPTMHAVQDLLNAHYLVQIMV 78
           ++VE+  N  I  PY+++   NYI+ VG      ++D +LP M ++QDLLNAHYL ++ +
Sbjct: 92  TLVEVWFNTYI-VPYVDDYTINYIV-VGDKAIPGLDDNILPVMKSLQDLLNAHYLGRVKL 151

Query: 79  TTMISYHSSLLIKDLPSRGTLYPNLAKHLKPVINFLAHQGS 116
           TT++ +  SL + + PS G  YP + ++++ ++ FL  QGS
Sbjct: 152 TTLVGF-KSLAVTNPPSSGAFYPTIVENMRGILKFLGRQGS 189

[Close]

BLAST of Lag0021897 vs. NCBI nr
Match: KAE8653445.1 (hypothetical protein Csa_006879 [Cucumis sativus])

HSP 1 Score: 70.1 bits (170), Expect = 1.5e-08
Identity = 40/120 (33.33%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 8   GVLDIAKISPNSVV-ELCHNYKIGQPYIN--------EINFNYIIFVGMDINDE---LLP 67
           G +D++   PN+++ +L +N  + + + N        +   NYII     I      +LP
Sbjct: 411 GAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILP 470

Query: 68  TMHAVQDLLNAHYLVQIMVTTMISYHSSLLIKDLPSRGTLYPNLAKHLKPVINFLAHQGS 116
            M ++QDLLNA+YL Q+ +TT++ Y+++L+ KD PS GT  P + + ++ ++ FLA +GS
Sbjct: 471 IMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGS 530

[Close]

BLAST of Lag0021897 vs. NCBI nr
Match: XP_004144124.2 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis sativus])

HSP 1 Score: 70.1 bits (170), Expect = 1.5e-08
Identity = 40/120 (33.33%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 8   GVLDIAKISPNSVV-ELCHNYKIGQPYIN--------EINFNYIIFVGMDINDE---LLP 67
           G +D++   PN+++ +L +N  + + + N        +   NYII     I      +LP
Sbjct: 73  GAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILP 132

Query: 68  TMHAVQDLLNAHYLVQIMVTTMISYHSSLLIKDLPSRGTLYPNLAKHLKPVINFLAHQGS 116
            M ++QDLLNA+YL Q+ +TT++ Y+++L+ KD PS GT  P + + ++ ++ FLA +GS
Sbjct: 133 IMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGS 192

The following BLAST results are available for this feature:

BLAST of Lag0021897 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136187.1	2.1e-10	38.10	probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia]	[more]
XP_038880473.1	8.1e-10	37.78	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
KAG7011054.1	5.2e-09	37.62	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]
KAE8653445.1	1.5e-08	33.33	hypothetical protein Csa_006879 [Cucumis sativus]	[more]
XP_004144124.2	1.5e-08	33.33	probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis sativus]	[more]

BLAST of Lag0021897 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 9..117 e-value: 2.7E-9 score: 38.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0021897.1	Lag0021897.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds