Lag0021830 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0021830
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
Location	chr7: 12746533 .. 12750570 (-)
RNA-Seq Expression	Lag0021830
Synteny	Lag0021830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACATGGAGTTGTGAAACAGTTGTTGAAAGGTGGAGTTATCTTCTTGGAGTATGTGAGGTCTGAGAGGAATTTGGCAGATCCTCTCACCAAAGGCCTAACAAGGAGAGTAATTCTAGAGTCCTCAACAAGTATGAGACTCAAACTGTTGAAATGAGTCCATAGCCCTTTATTTGGGTGGTCCAAATACGGACTTGATGGACACATTTTTGAAAAGTCTTCCTAGCTCAAGTTTTTGAGTGGTTTACGACGGTAGACTTGATGAAGCATGACGTGATTGTGAAAGTGTGGCTGCCTTCTATGAAATAGTGAAATGAATCTTTTTCTAGAGCTTTCTCGATGACCTAAGGTGTTTGTGTGCATGGCCCAAAATTGCACTTGTTCTACTATTGTATCGCGGAATCAAGGAAAACATGAGGATACAAACGTGTTGTAGGGGTCTAAACCATAACTATTGAAAGTTCAAAAGGTAACTTACTTTCACTATGCTGGTTATTGCCTTACTTCACTATGCGTTAATTCAAATCGAAAGATATTAATGCTTAAGTTTAAATTCCTCATTTGCTCATAAAATGTTGTTTATAGTCATTCGTGGGGATTGTTGGGAATGACTTAATCTTGGCCTAGTGTAGTGTGCATGAAGACTTTTGTCCCACATGGATAAGTTTAGAGTTGAGAAGAGTGTTCTCATACAATCACTCTTCCTTACTTTTGTAAAAGTGATTAATAGTGGAAGCAAGTGTTCACCCCACACGTGCGTGCTGCCGCCGCAGGTTGGACAGGTGGGTTCGGGTAATCACGCTCGCGCAAAATAAAGAGAGCGGTTTTGGTGTTAATTCCTCCTATAAAAGGCAGCAAAGCAAGCCTCTCGAGTGCACGAATGAGGATGTAGGTGTTGTATTAACCTTGGGGAGCAACCGGCGTAAACGATTTAGCACCGTGGTCGTGCCAAAATTGCTTTCAAGGTAATGGTACTTTCCACGACACAACAATCGTGTTTATCAATTTCTACTAAAAAAAATCAACCAACCGACGTCGGTTCGACCGATCGACTCAATTTTCGGATTGTTTTTGCTCACCCCTACTTTTACTTTTTGCTTAGAAAACATTTGAAACGATTTGTTGAAACCTAGCATAAATTTGAGAATATCTTGGGTTAAAAAAAATTCAGATTATTTTGTAAAATTTAACCATTTATTTATTTAATTTTTTTTTTCTTACAATACCATTTATTTATTTAAGTGGAGTCGAAGTTCACATTTATTTCTTATATTTTTATGACCGTAGACCTTAGAAACCACATTTGGAACTCGAGGCTCCTGAAGTTGCAGAAACTCCTCACGTCGGCCCAATTCTACCAGAAGAGAGAGAGAGATTGAGCCGAGGATTAACGCTGTATCTCTGAAATCTGAACTTATTCCGCTGCTTTTCCCTCTGTTATGATCGGCGGCTGCTGAATGCAAAGCTGCTCTTTGGACTTATTGTTCTTTTGCTATGGAGTTAGAATCGTACTACGAAATTCCCCGCACCGCCGACTTCATTCACAGCCGGAACTTCACCAGAGTCGCATTGCAGGTATTTCTTTGAATTCCCAAATACTGGAGGTTTCAAATGATTTTCACTTCTTTCTCTGCTCCTTATTGTCAAACGATGGACAGTTTCGCCTTGTGTTATGGTTCGAGTTTGCTGGGAATGTGTAAATGCTATATGGGATTTTCTCAAGTCTGCTTATTTTGAGTTGATTTTTCTTTTCTTTCTTTTTGGTATATGGTTATTCATGTTCTTCTGAGTGCCTTGACTAGTCTCTTAAACAGAGTTTTAAGCATGGACACTTGGAAGTTGAATTCTGACGCCCTTTTTCCACTTTTCTTTTTTACTTTGAAAAATATTAGTTCCCAGACGAGCTCTTGAAGGATTCAACTAGAGTGGTGAATGCTTTAAGGAGAAGGCTTCGTGCGCTTAATGATACAAACCAAAGCGATGGCGAAAATGAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGTTGTTGTGTTGATGAGGTTGGAGCAGCTCACGCTAATGCTGATTGTGTCATTCACTATGGACATACATGTCTGAGCCCGTGAGTACCGTTCTACTATGGACATGATGCTTTTTACGCAAGATTTTTATTTTCTTTTCACATTGCAGTGCCCTTAGAAATCAGCATCCTAAGTTATTGAGTCGTTTCTGCAAATTTGCGCCAGTGTTTGTGCTGTTGATTTTTCTACATTCGTGTGAGAAACTAAAGTAAGTAAAAAACTTGTAACCCTTAAGAAGTGGATTATGGAGGTATTATCTGTCATATTAAATAATTGGTTGGTGCATTTGAAATTATTCTTTGAACTATCGGCTTAATGCTTTAAAAGTCTGAAATACTATTGGCCTGGGAGGTATTATTATTAAATAATGTATTTTATATTATCAATCATCACGTAAGAGGCATCTACATGGTTATGCTAACCTTTTGGATGATTGGGCATTCCTCACCTTCCTCATGATTGAGCAAATGCTTACTTGTCTGATCATAATTGTACCTTCAGTAAAAATGGAGAAGTATTTGGGTGATGGTTGTAATATAAAGCCATGTGATCTAATGAGGCGTTTGTATGCTTGGCTTTTCTTATGATATGGAGTTGTGGATTACAGATTTTTGCTTGCAATGTTTCTTATTTATTCTTCATTGTTTGGTGTTCTTGGTCGATTTCTTTCTTAGTTTAACTTTAATTTGAATATTAATATTTATTTAGCTCCAAAATGATTGGTAGATCAGCAGATAGATAGTAAAGGTGTAATATACTGTGTATGCTATTGTTTATATGCTGCAATGTTTCAGACTGCATATGGCTTATGAGATCCATCACGTACAGTGATTGCTGGTTTACTATTAGCAGTCTTACAATACACTTTTGGTGTCTTTCAGGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATTCAATTGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTTGTCCAATGGAAAGCCTGTTTTGGTATGCATTCGTGTTTTTGAATCACTGTACTTCTTTTGGGGGGGGGGGGGGGGGAGATAACAATATGAAATTGATTAAGAAGAATGAGGGAACAGTACAAAGAAAACCATACCATACAAAGAAACGTAGAATGGAGCCAGGGCACCCAAATACCTGCAAAAAACTCTCCTACATCTTACAAAGATGTGATCCGAATCTTCAGATGTTTGGTTGCAAAAAGCACACCACTTGGTCCTGAGAAAAATCAAGAATGCTCTTTGGATAAATTCTATGGTGTCAACGCTCCCAGGAACCACAAGGTAAGAAGCTAAACTTCTTTGGGGATTTGCGGCTTGGATGAAACTTGCTAGAAGACTTGAGAGTCCAAACTCTCTTACCTCTAGATGTTGAGAAAGCCAAGAAGCCAAGCTGAATGAACAATAGAGAGGACAACTTAATCATCTCTCAATTAGAAGGACCTCTCCTAAAATTGAATATAGAGAGTGGCCTTTTGCTTGGAAGAGAAATAAAATTGAGGATTTGCTTGGGGTAAAAAAGAAATACAGATTGAATTGACACACTACTAACCCAACACCTCCCTGAGAATTGAATGATGTCCATCAACGACCGGAGTTAATGTGATAGAAAAAGAAGTGAATACTGTGAGGTGGCTTTTCTAGGATTCCTGAAAGCAATCTTATATGCTTACAATGATTCAAATCACTATACTTAAGATTTTAGAATGAACCAGTTTTACTTTGGGAAAAAAAGACCTTGTTCCACCTTTCATGCTCAATCCCTTTGGAAATGAGTTTTCAATTCCTATCTCTCGGTACTGTATGATTTCTATGTTGATGTTAACTGTGTTTTGAAAGCACATCTAGGTGCTTTTTCTAGAAAAACCTTGCGAAGAAGCCTTGCTTCTTTCAATGAAGCACACTGAGGTGCAAGCCTTAGCAGTTGAATCTGATGTAGCCGATTATATGTTCATTAATTAG

mRNA sequence

ATGAGACATGGAGTTGTGAAACAGTTGTTGAAAGGTGGAGTTATCTTCTTGGAGTATGTGAGGTCTGAGAGGAATTTGGCAGATCCTCTCACCAAAGGCCTAACAAGGAGAGTAATTCTAGAGTCCTCAACAATGGAAGCAAGTGTTCACCCCACACGTGCGTGCTGCCGCCGCAGGTTGGACAGGTGGGTTCGGGTAATCACGCTCGCGCAAAATAAAGAGAGCGGTTTTGGTGTTAATTCCTCCTATAAAAGGCAGCAAAGCAAGCCTCTCGAGTGCACGAATGAGGATGTAGGTGTTGTATTAACCTTGGGGAGCAACCGGCGTAAACGATTTAGCACCGTGGTCGTGCCAAAATTGCTTTCAAGAATCGTACTACGAAATTCCCCGCACCGCCGACTTCATTCACAGCCGGAACTTCACCAGAGTCGCATTGCAGGTATTTCTTTGAATTCCCAAATACTGGAGTTCCCAGACGAGCTCTTGAAGGATTCAACTAGAGTGGTGAATGCTTTAAGGAGAAGGCTTCGTGCGCTTAATGATACAAACCAAAGCGATGGCGAAAATGAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGTTGTTGTGTTGATGAGGTTGGAGCAGCTCACGCTAATGCTGATTGTGTCATTCACTATGGACATACATGTCTGAGCCCTGCCCTTAGAAATCAGCATCCTAAGTTATTGAGTCGTTTCTGCAAATTTGCGCCAGTGTTTGTGCTGTTGATTTTTCTACATTCGTGTGAGAAACTAAAGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATTCAATTGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTTGTCCAATGGAAAGCCTGTTTTGGGCACCCAAATACCTGCAAAAAACTCTCCTACATCTTACAAAGATGTGATCCGAATCTTCAGATGTTTGGTTGCAAAAAGCACACCACTTGGTCCTGAGAAAAATCAAGAATGCTCTTTGGATAAATTCTATGGTGTCAACGCTCCCAGGAACCACAAGGTGCTTTTTCTAGAAAAACCTTGCGAAGAAGCCTTGCTTCTTTCAATGAAGCACACTGAGGTGCAAGCCTTAGCAGTTGAATCTGATGTAGCCGATTATATGTTCATTAATTAG

Coding sequence (CDS)

ATGAGACATGGAGTTGTGAAACAGTTGTTGAAAGGTGGAGTTATCTTCTTGGAGTATGTGAGGTCTGAGAGGAATTTGGCAGATCCTCTCACCAAAGGCCTAACAAGGAGAGTAATTCTAGAGTCCTCAACAATGGAAGCAAGTGTTCACCCCACACGTGCGTGCTGCCGCCGCAGGTTGGACAGGTGGGTTCGGGTAATCACGCTCGCGCAAAATAAAGAGAGCGGTTTTGGTGTTAATTCCTCCTATAAAAGGCAGCAAAGCAAGCCTCTCGAGTGCACGAATGAGGATGTAGGTGTTGTATTAACCTTGGGGAGCAACCGGCGTAAACGATTTAGCACCGTGGTCGTGCCAAAATTGCTTTCAAGAATCGTACTACGAAATTCCCCGCACCGCCGACTTCATTCACAGCCGGAACTTCACCAGAGTCGCATTGCAGGTATTTCTTTGAATTCCCAAATACTGGAGTTCCCAGACGAGCTCTTGAAGGATTCAACTAGAGTGGTGAATGCTTTAAGGAGAAGGCTTCGTGCGCTTAATGATACAAACCAAAGCGATGGCGAAAATGAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGTTGTTGTGTTGATGAGGTTGGAGCAGCTCACGCTAATGCTGATTGTGTCATTCACTATGGACATACATGTCTGAGCCCTGCCCTTAGAAATCAGCATCCTAAGTTATTGAGTCGTTTCTGCAAATTTGCGCCAGTGTTTGTGCTGTTGATTTTTCTACATTCGTGTGAGAAACTAAAGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATTCAATTGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTTGTCCAATGGAAAGCCTGTTTTGGGCACCCAAATACCTGCAAAAAACTCTCCTACATCTTACAAAGATGTGATCCGAATCTTCAGATGTTTGGTTGCAAAAAGCACACCACTTGGTCCTGAGAAAAATCAAGAATGCTCTTTGGATAAATTCTATGGTGTCAACGCTCCCAGGAACCACAAGGTGCTTTTTCTAGAAAAACCTTGCGAAGAAGCCTTGCTTCTTTCAATGAAGCACACTGAGGTGCAAGCCTTAGCAGTTGAATCTGATGTAGCCGATTATATGTTCATTAATTAG

Protein sequence

MRHGVVKQLLKGGVIFLEYVRSERNLADPLTKGLTRRVILESSTMEASVHPTRACCRRRLDRWVRVITLAQNKESGFGVNSSYKRQQSKPLECTNEDVGVVLTLGSNRRKRFSTVVVPKLLSRIVLRNSPHRRLHSQPELHQSRIAGISLNSQILEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNGKPVLGTQIPAKNSPTSYKDVIRIFRCLVAKSTPLGPEKNQECSLDKFYGVNAPRNHKVLFLEKPCEEALLLSMKHTEVQALAVESDVADYMFIN

Homology

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BLAST of Lag0021830 vs. NCBI nr
Match: KAG7029112.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 199.1 bits (505), Expect = 6.8e-47
Identity = 104/144 (72.22%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 26  LQFPDELLKDSTRVVSALRKRLCALNDTNQSDGENKVRLFIMADTTYGSCCVDEVGAAHA 85

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 86  NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 136

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 146 DPIDVSTCAKTLSHYSSSNGKPVL 136

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BLAST of Lag0021830 vs. NCBI nr
Match: KAG6597669.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 199.1 bits (505), Expect = 6.8e-47
Identity = 104/144 (72.22%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 60  LQFPDELLKDSTRVVSALRKRLCALNDTNQSDGENKVRLFIMADTTYGSCCVDEVGAAHA 119

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 120 NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 170

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 180 DPIDVSTCAKTLSHYSSSNGKPVL 170

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BLAST of Lag0021830 vs. NCBI nr
Match: XP_022932622.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita moschata])

HSP 1 Score: 199.1 bits (505), Expect = 6.8e-47
Identity = 104/144 (72.22%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 26  LQFPDELLKDSTRVVSALRKRLCALNDTNQSDGENKVRLFIMADTTYGSCCVDEVGAAHA 85

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 86  NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 136

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 146 DPIDVSTCAKTLSHYSSSNGKPVL 136

[Close]

BLAST of Lag0021830 vs. NCBI nr
Match: XP_023541019.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.1 bits (505), Expect = 6.8e-47
Identity = 104/144 (72.22%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 26  LQFPDELLKDSTRVVSALRKRLCALNDTNQSDGENKVRLFIMADTTYGSCCVDEVGAAHA 85

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 86  NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 136

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 146 DPIDVSTCAKTLSHYSSSNGKPVL 136

[Close]

BLAST of Lag0021830 vs. NCBI nr
Match: XP_022972272.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita maxima])

HSP 1 Score: 194.1 bits (492), Expect = 2.2e-45
Identity = 102/144 (70.83%), Postives = 106/144 (73.61%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALND NQSD EN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 26  LQFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVRLFIMADTTYGSCCVDEVGAAHA 85

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 86  NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 136

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 146 DPIDVSTCAKTLSHYSSSNGKPVL 136

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BLAST of Lag0021830 vs. ExPASy Swiss-Prot
Match: A4QN59 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Danio rerio OX=7955 GN=dph2 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 7.2e-15
Identity = 46/131 (35.11%), Postives = 62/131 (47.33%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELL D+ RV             + + + + + + +I+ DT+YGSCCVDEV A H 
Sbjct: 52  LQFPDELLPDAVRV-------------SAEIEDKTKAKTYILGDTSYGSCCVDEVAAEHV 111

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
            ADC++HYG +CLSP  R                                 LP  +VFGK
Sbjct: 112 GADCIVHYGSSCLSPCRR---------------------------------LPLLYVFGK 136

Query: 275 DSIDVSTCAKT 286
             IDV  CA +
Sbjct: 172 RPIDVHQCASS 136

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BLAST of Lag0021830 vs. ExPASy Swiss-Prot
Match: Q4I5M4 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=DPH2 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 9.4e-15
Identity = 36/75 (48.00%), Postives = 54/75 (72.00%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPD +L D+ RVV AL +    +N  +Q + + E R++++AD++Y +CCVDE+ A H 
Sbjct: 60  LQFPDFMLVDAPRVVEALSKE---INTHDQEEAKPERRIYVLADSSYSACCVDEIAAEHV 119

Query: 215 NADCVIHYGHTCLSP 230
           +AD V+HYG TCLSP
Sbjct: 120 SADVVVHYGRTCLSP 131

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BLAST of Lag0021830 vs. ExPASy Swiss-Prot
Match: Q5B2Q1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=dph2 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 6.1e-14
Identity = 47/118 (39.83%), Postives = 64/118 (54.24%), Query Frame = 0


Query: 139 ELHQSRIAGISLNSQILEFPDELLKDSTRVVNALRRRL----------RALNDTNQSDG- 198
           E+ Q+R   I+     L+FPDE+L D+ RV   L R L          +A  +T  +DG 
Sbjct: 50  EIRQARYKRIA-----LQFPDEMLPDAPRVFQLLSRGLEARDIVDGGSKANTETEPTDGL 109

Query: 199 -------------ENEVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALR 233
                        E   +L+I+ADT+YG+CCVDEV A H +AD V+HYG +CLSP  R
Sbjct: 110 VDSASRLDLKDADEWSPKLYILADTSYGTCCVDEVAAEHVDADVVVHYGRSCLSPTAR 162

[Close]

BLAST of Lag0021830 vs. ExPASy Swiss-Prot
Match: Q59SJ9 (Diphthamide biosynthesis protein 2-1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=DPH2 PE=3 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 1.8e-13
Identity = 35/76 (46.05%), Postives = 51/76 (67.11%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEV--RLFIMADTTYGSCCVDEVGAA 214
           L+FPD L+ DS  +V+ L+R L  +   NQ  GE+    R++I+ADT+Y +CCVDEV A 
Sbjct: 78  LQFPDNLICDSATIVHELQRELNIVPQANQDTGESNTAQRVWILADTSYSACCVDEVAAE 137

Query: 215 HANADCVIHYGHTCLS 229
           H  +D V+H+G  CL+
Sbjct: 138 HVRSDLVVHFGDACLN 153

[Close]

BLAST of Lag0021830 vs. ExPASy Swiss-Prot
Match: A0A1D8PL26 (Diphthamide biosynthesis protein 2-2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CAALFM_C400490WA PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.8e-13
Identity = 35/76 (46.05%), Postives = 51/76 (67.11%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEV--RLFIMADTTYGSCCVDEVGAA 214
           L+FPD L+ DS  +V+ L+R L  +   NQ  GE+    R++I+ADT+Y +CCVDEV A 
Sbjct: 78  LQFPDNLICDSATIVHELQRELNIVPQANQDTGESNTAQRVWILADTSYSACCVDEVAAE 137

Query: 215 HANADCVIHYGHTCLS 229
           H  +D V+H+G  CL+
Sbjct: 138 HVRSDLVVHFGDACLN 153

[Close]

BLAST of Lag0021830 vs. ExPASy TrEMBL
Match: A0A6J1EXA1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111439125 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.3e-47
Identity = 104/144 (72.22%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALNDTNQSDGEN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 26  LQFPDELLKDSTRVVSALRKRLCALNDTNQSDGENKVRLFIMADTTYGSCCVDEVGAAHA 85

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 86  NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 136

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 146 DPIDVSTCAKTLSHYSSSNGKPVL 136

[Close]

BLAST of Lag0021830 vs. ExPASy TrEMBL
Match: A0A6J1I4D3 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111470856 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.1e-45
Identity = 102/144 (70.83%), Postives = 106/144 (73.61%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDSTRVV+ALR+RL ALND NQSD EN+VRLFIMADTTYGSCCVDEVGAAHA
Sbjct: 26  LQFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVRLFIMADTTYGSCCVDEVGAAHA 85

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           NADCVIHYGHTCLSP                                 TTTLPAFFVFGK
Sbjct: 86  NADCVIHYGHTCLSP---------------------------------TTTLPAFFVFGK 136

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
           D IDVSTCAKTLSHYS SNGKPVL
Sbjct: 146 DPIDVSTCAKTLSHYSSSNGKPVL 136

[Close]

BLAST of Lag0021830 vs. ExPASy TrEMBL
Match: A0A5A7TMM8 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003010 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.5e-42
Identity = 97/146 (66.44%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAA 214
           L+FPDELLKDSTRVV AL+ RLR L  +DTN SD +NEVRLF+MADTTYGSCCVDEVGAA
Sbjct: 26  LQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDSKNEVRLFVMADTTYGSCCVDEVGAA 85

Query: 215 HANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVF 274
           HANADCVIHYGHTCLSP                                 TTTLPA FVF
Sbjct: 86  HANADCVIHYGHTCLSP---------------------------------TTTLPALFVF 138

Query: 275 GKDSIDVSTCAKTLSHYSLSNGKPVL 299
           GKDSIDV+TCAKTLS YSLS+GKPVL
Sbjct: 146 GKDSIDVTTCAKTLSQYSLSSGKPVL 138

[Close]

BLAST of Lag0021830 vs. ExPASy TrEMBL
Match: A0A5D3D327 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001750 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.5e-42
Identity = 97/146 (66.44%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAA 214
           L+FPDELLKDSTRVV AL+ RLR L  +DTN SD +NEVRLF+MADTTYGSCCVDEVGAA
Sbjct: 26  LQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDSKNEVRLFVMADTTYGSCCVDEVGAA 85

Query: 215 HANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVF 274
           HANADCVIHYGHTCLSP                                 TTTLPA FVF
Sbjct: 86  HANADCVIHYGHTCLSP---------------------------------TTTLPALFVF 138

Query: 275 GKDSIDVSTCAKTLSHYSLSNGKPVL 299
           GKDSIDV+TCAKTLS YSLS+GKPVL
Sbjct: 146 GKDSIDVTTCAKTLSQYSLSSGKPVL 138

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BLAST of Lag0021830 vs. ExPASy TrEMBL
Match: A0A1S3BYG9 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucumis melo OX=3656 GN=LOC103494584 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.5e-42
Identity = 97/146 (66.44%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRAL--NDTNQSDGENEVRLFIMADTTYGSCCVDEVGAA 214
           L+FPDELLKDSTRVV AL+ RLR L  +DTN SD +NEVRLF+MADTTYGSCCVDEVGAA
Sbjct: 26  LQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDSKNEVRLFVMADTTYGSCCVDEVGAA 85

Query: 215 HANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVF 274
           HANADCVIHYGHTCLSP                                 TTTLPA FVF
Sbjct: 86  HANADCVIHYGHTCLSP---------------------------------TTTLPALFVF 138

Query: 275 GKDSIDVSTCAKTLSHYSLSNGKPVL 299
           GKDSIDV+TCAKTLS YSLS+GKPVL
Sbjct: 146 GKDSIDVTTCAKTLSQYSLSSGKPVL 138

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BLAST of Lag0021830 vs. TAIR 10
Match: AT3G59630.1 (diphthamide synthesis DPH2 family protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-29
Identity = 68/144 (47.22%), Postives = 86/144 (59.72%), Query Frame = 0


Query: 155 LEFPDELLKDSTRVVNALRRRLRALNDTNQSDGENEVRLFIMADTTYGSCCVDEVGAAHA 214
           L+FPDELLKDST+VV+AL+ + R L D        EVR F+MADTTYGSCC+DEVGA H 
Sbjct: 28  LQFPDELLKDSTKVVSALKSKTRLLTD-------REVRFFVMADTTYGSCCIDEVGALHI 87

Query: 215 NADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLIFLHSCEKLKTTTLPAFFVFGK 274
           +++CV+HYG TCLSP                                 T+ LPAFFVFGK
Sbjct: 88  DSECVVHYGQTCLSP---------------------------------TSVLPAFFVFGK 131

Query: 275 DSIDVSTCAKTLSHYSLSNGKPVL 299
            SI+VS+C K L  Y+  + KP++
Sbjct: 148 ASINVSSCVKHLIDYASKSDKPIM 131

[Close]

BLAST of Lag0021830 vs. TAIR 10
Match: AT5G62030.1 (diphthamide synthesis DPH2 family protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.3e-08
Identity = 42/166 (25.30%), Postives = 72/166 (43.37%), Query Frame = 0


Query: 194 FIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPALRNQHPKLLSRFCKFAPVFVLLI 253
           F++ D TYG+CCVD+  A    AD +IHYGH+CL P                        
Sbjct: 95  FVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVP------------------------ 154

Query: 254 FLHSCEKLKTTTLPAFFVFGKDSIDVSTCAKTLSHYSLSNG--KPVLGTQIPAKNSPTSY 313
                  + +T +P  +VF +  IDV     T+ H +L++     +L   I   ++  + 
Sbjct: 155 -------IDSTKIPCLYVFVEIQIDVKCLLNTI-HLNLASDVKNIILAGTIQFTSAIRAV 214

Query: 314 KDVI--RIFRCLVAKSTPLGPEKNQECSLDKFYGVNAPRNHKVLFL 356
           K  +  + F  L+ +S PL   +   C+  K   V   ++  ++F+
Sbjct: 215 KPELEKQGFNVLIPQSKPLSAGEVLGCTAPKVKTVEDCKDQVLVFV 228

The following BLAST results are available for this feature:

BLAST of Lag0021830 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029112.1	6.8e-47	72.22	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita argyrosperma ...	[more]
KAG6597669.1	6.8e-47	72.22	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2, partial [Cucurbita argy...	[more]
XP_022932622.1	6.8e-47	72.22	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita moschata]	[more]
XP_023541019.1	6.8e-47	72.22	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita pepo subsp. p...	[more]
XP_022972272.1	2.2e-45	70.83	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Cucurbita maxima]	[more]

BLAST of Lag0021830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4QN59	7.2e-15	35.11	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Danio rerio OX=7955 G...	[more]
Q4I5M4	9.4e-15	48.00	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Gibberella zeae (stra...	[more]
Q5B2Q1	6.1e-14	39.83	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Emericella nidulans (...	[more]
Q59SJ9	1.8e-13	46.05	Diphthamide biosynthesis protein 2-1 OS=Candida albicans (strain SC5314 / ATCC M...	[more]
A0A1D8PL26	1.8e-13	46.05	Diphthamide biosynthesis protein 2-2 OS=Candida albicans (strain SC5314 / ATCC M...	[more]

BLAST of Lag0021830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EXA1	3.3e-47	72.22	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucurbita moschata OX...	[more]
A0A6J1I4D3	1.1e-45	70.83	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucurbita maxima OX=3...	[more]
A0A5A7TMM8	5.5e-42	66.44	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucumis melo var. mak...	[more]
A0A5D3D327	5.5e-42	66.44	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucumis melo var. mak...	[more]
A0A1S3BYG9	5.5e-42	66.44	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 OS=Cucumis melo OX=3656 ...	[more]

BLAST of Lag0021830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59630.1	1.1e-29	47.22	diphthamide synthesis DPH2 family protein	[more]
AT5G62030.1	1.3e-08	25.30	diphthamide synthesis DPH2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 162..182
IPR016435	Diphthamide synthesis DPH1/DPH2	TIGRFAM	TIGR00322	TIGR00322	coord: 154..315 e-value: 8.9E-31 score: 105.4
IPR016435	Diphthamide synthesis DPH1/DPH2	PFAM	PF01866	Diphthamide_syn	coord: 157..231 e-value: 1.1E-22 score: 80.9
IPR016435	Diphthamide synthesis DPH1/DPH2	SFLD	SFLDS00032	Radical_SAM_3-amino-3-carboxy	coord: 155..231 e-value: 5.1E-34 score: 111.6
IPR016435	Diphthamide synthesis DPH1/DPH2	PANTHER	PTHR10762	DIPHTHAMIDE BIOSYNTHESIS PROTEIN	coord: 155..298
IPR042263	Diphthamide synthesis DPH1/DPH2, domain 1	GENE3D	3.40.50.11840	Diphthamide synthesis DPH1/DPH2 domain 1	coord: 142..241 e-value: 7.0E-25 score: 88.9
IPR010014	Diphthamide synthesis DHP2	PANTHER	PTHR10762:SF2	2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2	coord: 155..298

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0021830.1	Lag0021830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
molecular_function	GO:0016740	transferase activity