Lag0021725 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0021725 vs. NCBI nr
Match: KAF7127078.1 (hypothetical protein RHSIM_Rhsim11G0146500 [Rhododendron simsii])

HSP 1 Score: 197.6 bits (501), Expect = 1.3e-46
Identity = 95/178 (53.37%), Postives = 118/178 (66.29%), Query Frame = 0

Query: 83  SNRQCPNEGRRLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVM 142
           S  Q    G R IT+NV+++ E  H I  KRSG +G    KLDM KAYDRV+W YL++VM
Sbjct: 449 SENQSAFVGGRQITDNVLIAHELTHYIRHKRSGTKGVAAFKLDMAKAYDRVDWRYLEQVM 508

Query: 143 DIMGFSGMWRNKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFST 202
             +GF  +W   VM C+ +  FSI VN    E F PSRGIRQG  LSPYLFLLC EGF+ 
Sbjct: 509 RQLGFHELWIGWVMECVKTVSFSITVNGEAGESFHPSRGIRQGCPLSPYLFLLCGEGFTA 568

Query: 203 LQNREESISNLYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           L N   S   LYGFRIN+HCP +SHL FADDS+++C+A E+DC  +  ++ LY  ASG
Sbjct: 569 LCNGYVSKKLLYGFRINRHCPVVSHLLFADDSVVYCQATERDCAALYNILELYEAASG 626

BLAST of Lag0021725 vs. NCBI nr
Match: KAF7129689.1 (hypothetical protein RHSIM_Rhsim10G0093500 [Rhododendron simsii])

HSP 1 Score: 197.6 bits (501), Expect = 1.3e-46
Identity = 95/178 (53.37%), Postives = 118/178 (66.29%), Query Frame = 0

Query: 83  SNRQCPNEGRRLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVM 142
           S  Q    G R IT+NV+++ E  H I  KRSG +G    KLDM KAYDRV+W YL++VM
Sbjct: 449 SENQSAFVGGRQITDNVLIAHELTHYIRHKRSGTKGVAAFKLDMAKAYDRVDWRYLEQVM 508

Query: 143 DIMGFSGMWRNKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFST 202
             +GF  +W   VM C+ +  FSI VN    E F PSRGIRQG  LSPYLFLLC EGF+ 
Sbjct: 509 RQLGFHELWIGWVMECVKTVSFSITVNGEAGESFHPSRGIRQGCPLSPYLFLLCGEGFTA 568

Query: 203 LQNREESISNLYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           L N   S   LYGFRIN+HCP +SHL FADDS+++C+A E+DC  +  ++ LY  ASG
Sbjct: 569 LCNDYVSKKLLYGFRINRHCPVVSHLLFADDSVVYCQATERDCAALYNILELYEAASG 626

BLAST of Lag0021725 vs. NCBI nr
Match: XP_030931068.1 (uncharacterized protein LOC115956947 [Quercus lobata])

HSP 1 Score: 196.1 bits (497), Expect = 3.9e-46
Identity = 100/218 (45.87%), Postives = 138/218 (63.30%), Query Frame = 0

Query: 49  PKKRGPQDLPE-----LIDLRHSPPSRLAISRDGKI--TLKSNRQCPNEGRRLITNNVVM 108
           PKK+ P +L +     L ++ +   S+   +R  KI   L S  Q      RLIT+N+++
Sbjct: 374 PKKKSPTNLKDYRPISLCNVIYKIISKAIANRLKKILPKLVSETQSAFMSDRLITDNILV 433

Query: 109 SFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWRNKVMRCIFS 168
           +FE +H +  KR GK GF+ +KLDM+KAYDRVEW +LDK+++I+GF   WRN V  C  S
Sbjct: 434 AFETLHHLKNKRKGKTGFMALKLDMSKAYDRVEWAFLDKIIEILGFDDKWRNLVGCCFRS 493

Query: 169 AFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFRINKH 228
             FS+++N      F PSRG+RQGD LSPYLFLLCAEG  +L  +      L G  + K 
Sbjct: 494 VSFSVMINGEPHGFFHPSRGLRQGDPLSPYLFLLCAEGLHSLIQQAADNGELRGVSLCKE 553

Query: 229 CPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKAS 260
            P I+HLFFADDSL+FCRA E DC+ +  ++ +Y +AS
Sbjct: 554 GPKITHLFFADDSLLFCRATESDCQKVLNILTVYEEAS 591

BLAST of Lag0021725 vs. NCBI nr
Match: KAF7116955.1 (hypothetical protein RHSIM_RhsimUnG0010200 [Rhododendron simsii])

HSP 1 Score: 195.7 bits (496), Expect = 5.1e-46
Identity = 99/178 (55.62%), Postives = 117/178 (65.73%), Query Frame = 0

Query: 83  SNRQCPNEGRRLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVM 142
           S  Q    G R IT+NV+++ E  H I  KRSG +G    KLDM KAYDRVEW YL++VM
Sbjct: 487 SENQSAFVGGRQITDNVLLAHELTHYIKHKRSGSQGVAAFKLDMAKAYDRVEWSYLERVM 546

Query: 143 DIMGFSGMWRNKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFST 202
             +GF   W   VM CI +  FSI VN    E F PSRGIRQG  LSPYLFLLC EGFS 
Sbjct: 547 AKLGFHQKWIGWVMECIRTVSFSITVNGEPGETFLPSRGIRQGCPLSPYLFLLCGEGFSA 606

Query: 203 LQNREESISNLYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           L     S   L GFRIN HCP ISHL FADDS+IFCRA+++DC  + ++++LY  ASG
Sbjct: 607 LFKDFLSKKMLRGFRINYHCPVISHLLFADDSIIFCRASDQDCEALYRILDLYEAASG 664

BLAST of Lag0021725 vs. NCBI nr
Match: XP_030498122.1 (uncharacterized protein LOC115713779 [Cannabis sativa])

HSP 1 Score: 194.1 bits (492), Expect = 1.5e-45
Identity = 85/169 (50.30%), Postives = 128/169 (75.74%), Query Frame = 0

Query: 92  RRLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMW 151
           +RLIT+NV+++FE +H++  ++ G +G+  +KLDM+KA+DRVEW Y+ ++M  MGF  + 
Sbjct: 556 QRLITDNVLIAFELLHSLKNRKRGSQGYAAIKLDMSKAFDRVEWNYIAQIMLKMGFGTII 615

Query: 152 RNKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESIS 211
            N ++RC+ S  +S L+N  +  + KPSRGIRQGD LSPYLFL+CAEG S L   +E+  
Sbjct: 616 VNLILRCLQSVSYSFLLNGKVLGDLKPSRGIRQGDPLSPYLFLICAEGLSRLLQSKENNG 675

Query: 212 NLYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           +LYG R+++  PS+SHLFFADDS++FCRAN +  R I++V+++Y +ASG
Sbjct: 676 SLYGLRVSRTAPSVSHLFFADDSVLFCRANPQSARSIQQVLDIYHRASG 724

BLAST of Lag0021725 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-11
Identity = 46/163 (28.22%), Postives = 77/163 (47.24%), Query Frame = 0

Query: 98  NVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWRNKVMR 157
           N+  S   IH IN  +   +  +++ LD  KA+D+++  ++ KV++  G  G + N +  
Sbjct: 582 NIRKSINVIHYINKLKD--KNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKA 641

Query: 158 CIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFR 217
                  +I VN    E      G RQG  LSPYLF +  E  +    +++ I    G +
Sbjct: 642 IYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKEIK---GIQ 701

Query: 218 INKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           I K    IS L  ADD +++    +   R +  +IN +G+  G
Sbjct: 702 IGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVG 737

BLAST of Lag0021725 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 7.2e-11
Identity = 31/57 (54.39%), Postives = 39/57 (68.42%), Query Frame = 0

Query: 178 PSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFRINKHCPSISHLFFADDS 235
           PSRG+RQGD LSPYLF+LC E  S L  R +    L G R++ + P I+HL FADD+
Sbjct: 24  PSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINHLLFADDT 80

BLAST of Lag0021725 vs. ExPASy Swiss-Prot
Match: Q05118 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.2e-07
Identity = 38/130 (29.23%), Postives = 62/130 (47.69%), Query Frame = 0

Query: 107 HTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWRNKVMRCIFSAFFSI 166
           H I  +R   + + V+ LD+ KA+D V    + + M   G     ++ +M  I  A+ +I
Sbjct: 11  HYIKLRRLKGKTYNVVSLDIRKAFDTVSHPAILRAMRAFGIDDGMQDFIMSTITDAYTNI 70

Query: 167 LVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFRINKHCPSIS 226
           +V    + +     G++QGD LSP LF +  +   T  N E+      G  +   C  I+
Sbjct: 71  VVGGRTTNKIYIRNGVKQGDPLSPVLFNIVLDELVTRLNDEQP-----GASMTPAC-KIA 130

Query: 227 HLFFADDSLI 237
            L FADD L+
Sbjct: 131 SLAFADDLLL 134

BLAST of Lag0021725 vs. ExPASy Swiss-Prot
Match: P0CV25 (Secreted RxLR effector protein 78 OS=Plasmopara viticola OX=143451 GN=RXLR78 PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.0e-04
Identity = 30/79 (37.97%), Postives = 41/79 (51.90%), Query Frame = 0

Query: 120 IVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWRNKVMRCIFSAFFSILVNDNLSEEFKPS 179
           +++ LD  KAYD V   +L  V+    FS M+   + +         LVN  LSE  +  
Sbjct: 28  VILLLDFKKAYDSVAREFLFLVLLRFEFSPMFVRMLRKLHDGTTARFLVNGELSEPQEVV 87

Query: 180 RGIRQGDLLSPYLFLLCAE 199
            GIRQG  L+P LF+L AE
Sbjct: 88  SGIRQGCSLAPLLFILAAE 106

BLAST of Lag0021725 vs. ExPASy TrEMBL
Match: A0A803QCS5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.4e-46
Identity = 85/168 (50.60%), Postives = 124/168 (73.81%), Query Frame = 0

Query: 93  RLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWR 152
           RLIT+N++++FE +H I  K +G+ G   +KLDM+KA+DRVEW ++ +VM  MGF+  W 
Sbjct: 392 RLITDNILVAFELVHAIKNKTAGRSGIATLKLDMSKAFDRVEWRFIQEVMVKMGFATKWV 451

Query: 153 NKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISN 212
           N +M C+ +  F+ L+N  ++    PSRG+RQG  LSPYLFL+C+EG S L ++E+S+ N
Sbjct: 452 NLIMGCLTTNTFTFLLNGEVTGSLTPSRGLRQGCPLSPYLFLICSEGLSRLLHQEQSMGN 511

Query: 213 LYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           L GF++ +H P ISHL FADDSL+FC+ANE  C  IK+V+++Y +ASG
Sbjct: 512 LNGFKLTRHAPPISHLLFADDSLLFCQANENSCLAIKRVLDIYHRASG 559

BLAST of Lag0021725 vs. ExPASy TrEMBL
Match: A0A803QC75 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.9e-46
Identity = 98/220 (44.55%), Postives = 143/220 (65.00%), Query Frame = 0

Query: 49   PKKRGPQDLPE-----LIDLRHSPPSRLAISRDGKITLK---SNRQCPNEGRRLITNNVV 108
            PK + PQ + E     L ++     +++ +SR  K+ L    S  Q      RLIT+NV+
Sbjct: 812  PKVKKPQHIQEYRPISLCNVISKLVTKVLVSR-FKLALPLVISEEQSAFLPNRLITDNVL 871

Query: 109  MSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWRNKVMRCIF 168
            ++FE +H I  K +G+ G    KLDM+KA+DRVEW ++++VM  MGF+  W + +M C+ 
Sbjct: 872  VAFELVHAIKNKTAGRNGVASFKLDMSKAFDRVEWKFIEEVMRKMGFAERWISLIMSCLT 931

Query: 169  SAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFRINK 228
            +  FS ++N  +     PSRG+RQG  LSPYLFL+C+EGFS L   E+  +NL GF++ +
Sbjct: 932  TNNFSFIINGEVMGNLTPSRGLRQGCPLSPYLFLICSEGFSRLLQHEQDSNNLMGFKLTR 991

Query: 229  HCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
            H P I+HLFFADDSL+FC+ANE+ C  IK+V++ Y KASG
Sbjct: 992  HAPPITHLFFADDSLLFCQANERSCLAIKRVLDTYHKASG 1030

BLAST of Lag0021725 vs. ExPASy TrEMBL
Match: A0A803P996 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 3.2e-46
Identity = 98/220 (44.55%), Postives = 143/220 (65.00%), Query Frame = 0

Query: 49   PKKRGPQDLPE-----LIDLRHSPPSRLAISRDGKITLK---SNRQCPNEGRRLITNNVV 108
            PK + PQ + E     L ++     +++ +SR  KI L    S  Q      RLIT+NV+
Sbjct: 909  PKVKKPQHIQEYRPISLCNVISKLVTKVLVSR-FKIALPMVISEEQSAFLPNRLITDNVL 968

Query: 109  MSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWRNKVMRCIF 168
            ++FE +H I  K +G+ G    KLDM+KA+DRVEW ++++VM  MGF+  W + +M C+ 
Sbjct: 969  VAFELVHAIKNKTAGRNGVASFKLDMSKAFDRVEWKFIEEVMRKMGFAERWISLIMSCLT 1028

Query: 169  SAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFRINK 228
            +  FS ++N  +  +  PSRG++QG  LSPYLFL+C+EGFS L   E+   NL GF++ +
Sbjct: 1029 TNNFSFIINGEVMGDLTPSRGLQQGCPLSPYLFLICSEGFSRLLQHEQDSHNLMGFKLTR 1088

Query: 229  HCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
            H P I+HLFFADDSL+FC+ANE+ C  IK+V++ Y KASG
Sbjct: 1089 HAPPITHLFFADDSLLFCQANERSCLAIKRVLDTYHKASG 1127

BLAST of Lag0021725 vs. ExPASy TrEMBL
Match: A0A803QAN3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-45
Identity = 86/168 (51.19%), Postives = 120/168 (71.43%), Query Frame = 0

Query: 93  RLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWR 152
           RLIT+N++++FE IH +  K  G++GF  +KLDM+KA+DRVEW YL+ VM  MGF+  W 
Sbjct: 403 RLITDNILVAFELIHCLRHKTQGRQGFSALKLDMSKAFDRVEWKYLEAVMLKMGFASKWV 462

Query: 153 NKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISN 212
             +M CI ++ FS  +N  +    +PSRG+RQGD LSPYLFL+C+EG S L   EE++ N
Sbjct: 463 TLIMNCICTSSFSFSLNGEVVGHVQPSRGLRQGDPLSPYLFLICSEGLSRLLQYEEAVGN 522

Query: 213 LYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           L G R+ +H PS+SHL FADDSL+FCRAN +    I++ ++ Y +ASG
Sbjct: 523 LEGLRLTRHSPSVSHLLFADDSLLFCRANNQSASAIQRTLDTYHQASG 570

BLAST of Lag0021725 vs. ExPASy TrEMBL
Match: A0A803PNH7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.6e-45
Identity = 85/168 (50.60%), Postives = 122/168 (72.62%), Query Frame = 0

Query: 93  RLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWR 152
           RLIT+NV+++FE ++ I  K SG++G   +KLDM+KA+DRVEW +++KVM  MGF+  W 
Sbjct: 558 RLITDNVLVAFELVNAIKNKTSGRKGVASLKLDMSKAFDRVEWNFIEKVMGKMGFASDWI 617

Query: 153 NKVMRCIFSAFFSILVNDNLSEEFKPSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISN 212
             +M C+ +  FS ++N  ++    PSRG+RQG  LSPYLFL+C+E  S L   EE + +
Sbjct: 618 RLIMSCLRTNSFSFILNGEVTGSLLPSRGLRQGCPLSPYLFLICSEALSRLLQHEEEVGH 677

Query: 213 LYGFRINKHCPSISHLFFADDSLIFCRANEKDCRIIKKVINLYGKASG 261
           L GF++ +H PSISHLFFADDSL+FC+ANE  C  +K+ +++Y KASG
Sbjct: 678 LNGFKLTRHAPSISHLFFADDSLLFCQANESSCLALKRSLDIYHKASG 725

BLAST of Lag0021725 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 69.3 bits (168), Expect = 5.1e-12
Identity = 31/57 (54.39%), Postives = 39/57 (68.42%), Query Frame = 0

Query: 178 PSRGIRQGDLLSPYLFLLCAEGFSTLQNREESISNLYGFRINKHCPSISHLFFADDS 235
           PSRG+RQGD LSPYLF+LC E  S L  R +    L G R++ + P I+HL FADD+
Sbjct: 24  PSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINHLLFADDT 80

BLAST of Lag0021725 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 60.1 bits (144), Expect = 3.1e-09
Identity = 26/70 (37.14%), Postives = 45/70 (64.29%), Query Frame = 0

Query: 93  RLITNNVVMSFECIHTINTKRSGKEGFIVMKLDMNKAYDRVEWYYLDKVMDIMGFSGMWR 152
           R+ T+N+V   E +H++  K+ G +G++++KLD+ KAYDR+ W YL+  +   GF  +W 
Sbjct: 24  RVSTDNIVFVQEAVHSMRRKK-GVKGWMLLKLDLEKAYDRIRWDYLEDTLISAGFPEVWL 83

Query: 153 NKVMRCIFSA 163
            ++ R  F A
Sbjct: 84  PEIARSTFGA 92