Homology
BLAST of Lag0021704 vs. NCBI nr
Match:
XP_038877313.1 (putative disease resistance protein RGA3 [Benincasa hispida])
HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 684/1065 (64.23%), Postives = 804/1065 (75.49%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLW FAVQE LKK VK+AAE+I +AWG +KELSKL LLK+EAILRDI++K+LHHE
Sbjct: 124 MAEFLWIFAVQEVLKKTVKVAAEEISMAWGFKKELSKLRKCLLKSEAILRDINKKKLHHE 183
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SVRMWVE+LQH VYE DDLLDELVYE LRQKV+T+ +KVR FFS S+N F FR KM K
Sbjct: 184 SVRMWVEDLQHFVYEVDDLLDELVYEHLRQKVDTEKRNKKVRNFFSSSNNPFPFRRKMAK 243
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETNKNI-LKQIRETTSTLNFE--VIGREVE 180
++K++TETL + Y EAS G+VAL+G+ES ET+KNI LKQIRETTS LNFE +IGREVE
Sbjct: 244 RMKDITETLYKHYCEASPLGLVALLGEESIETHKNINLKQIRETTSNLNFEEVIIGREVE 303
Query: 181 VSNIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFI 240
VSNIVK+VI+SSNEHPMSIIPIVGMGG+G+TTLAK VFNH+L+K HFDETIWVCVSEPFI
Sbjct: 304 VSNIVKMVIESSNEHPMSIIPIVGMGGLGETTLAKMVFNHELVKDHFDETIWVCVSEPFI 363
Query: 241 ITNILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCL 300
I NILE +F SLT TS+G +KENLL+ LQ+EMQGKSY LVLD VWNEEA LWGEL++CL
Sbjct: 364 ILNILEGMFQSLTKTSSGL-NKENLLRKLQEEMQGKSYLLVLDYVWNEEARLWGELRECL 423
Query: 301 KEIAGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELE 360
K IAGK GN IIVTTRS +VAT MET SSHHL KLSDDQCWSLFKESA+ANGL M+P+LE
Sbjct: 424 KNIAGKFGNSIIVTTRSAEVATVMETTSSHHLTKLSDDQCWSLFKESASANGLSMSPKLE 483
Query: 361 TIRVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSV 420
I+ ++++I GVPLVAKVLGGA FEGN+ERW+T+V+ VV NIS DDKDFV S LKLSV
Sbjct: 484 IIQKLVIRRIGGVPLVAKVLGGATKFEGNYERWLTQVESVVTNISNDDKDFVFSTLKLSV 543
Query: 421 DRLPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNF 480
D LPL SLKQCFAYCSNFPKD+ F KE+LIQMWIAQGF+Q Q+G++ M+ E+IGE+Y NF
Sbjct: 544 DCLPLFSLKQCFAYCSNFPKDYEFAKETLIQMWIAQGFIQAQKGNNTMLEENIGEDYINF 603
Query: 481 LLSRSLFEVV---IKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLIC 540
LLSRSLF+ V I G +THF MHDLIHDIAC IS+HQ E +N SEKS KLRT +
Sbjct: 604 LLSRSLFQDVSKDIDGTVTHFKMHDLIHDIACVISSHQNFEFKHSNWSEKSTRKLRTTVI 663
Query: 541 FKGYKVFNNDLKDHACLRVLVVD----RIRCDKNFSCLIGMFKHLRYLDISYISGYVELK 600
K+ ++ L D LRVLV++ RI D IGM KHLRYLD I G LK
Sbjct: 664 --NNKMIHDKLVDLGSLRVLVMNSPFLRILPDS-----IGMLKHLRYLD---IIGSSILK 723
Query: 601 DVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSA 660
ESI LYNLQTLKLG R+ LPKNLRKLV LRHLQFF I K+MPL +SQLIHLQTL A
Sbjct: 724 LPESITMLYNLQTLKLGTRVDLPKNLRKLVMLRHLQFFYIYIKQMPLCISQLIHLQTLPA 783
Query: 661 YEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSS 720
+ E+G KIEELGPL NLKGSL+L +L +VKSK EAI A+L+ KKNL +L W+ S
Sbjct: 784 FVVGSEKGCKIEELGPLNNLKGSLQLSSLNRVKSKKEAIAAKLMGKKNLCELTFEWDVYS 843
Query: 721 KEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEK 780
+E H NN + + F+VLEGLQPH NL+SLS+ Y GQV PN IFVE
Sbjct: 844 EEEKH-NNCN--------------NDFEVLEGLQPHKNLRSLSVVHYGGQVLPNGIFVEN 903
Query: 781 LVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNV------------------------- 840
LV IFL++C +CEMLPMLGQLPNLE L ISSM +V
Sbjct: 904 LVIIFLQECNRCEMLPMLGQLPNLEELHISSMGSVRSIGSEFYGNTHYSNQRVSFPKLKE 963
Query: 841 --------TKQWEDVSNGIAFPHLGILYIENCSKLMSVPHCFVSLHTLFLEGCKELRELP 900
+QWEDV++GIAFP+L L I CSKL+ VPH FVSL TL + GC+EL +LP
Sbjct: 964 LSFRLMQNLEQWEDVTDGIAFPYLQSLSIVICSKLLDVPHHFVSLRTLRIVGCEELAKLP 1023
Query: 901 NQLEGCNSIKCLKISDCPNLSVNVSNWSNLEAVEIIGCMQDYDISPFIHQSSI-THLSMT 960
N L NSI+ LKIS+CPNL++N+ N NL+ +EI+GCMQDYD SP +HQSSI THLS+
Sbjct: 1024 NGLNCPNSIESLKISECPNLALNLHNMHNLKEMEIVGCMQDYDFSPLLHQSSILTHLSLI 1083
Query: 961 DNSENNASQLPQQLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPS 1020
D S NA++LP+QL+++T LK L I FN +EALP+WLGNL SLE+L LF CKNL+ FPS
Sbjct: 1084 D-SLRNATELPRQLKYLTALKALTIKNFNSLEALPDWLGNLTSLETLGLFDCKNLKCFPS 1143
Query: 1021 KEVMLCLTKLNKLHVEGCPLRRHLCEGHSEWAKLSHLPKICVDVR 1022
KEVMLCL KLN LHV GCP+ L + E AKLSHLP CVDVR
Sbjct: 1144 KEVMLCLKKLNHLHVRGCPMLL-LGQDDPEKAKLSHLPNNCVDVR 1160
BLAST of Lag0021704 vs. NCBI nr
Match:
XP_022145229.1 (putative disease resistance protein RGA4 [Momordica charantia])
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 668/1059 (63.08%), Postives = 781/1059 (73.75%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLWTFAVQE LKK++KLAAEQIGVAW L+KELSKL LLKAE IL DI+R++L HE
Sbjct: 1 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+WVE+LQH VY+ADDLLD+LVYEDLRQKVE + KVR F SPS+N FLFR+KM K
Sbjct: 61 SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKI-NKVRNFLSPSNNPFLFRFKMAK 120
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETN-KNILKQIRETTSTLNFEVIGREVEVS 180
++K ++ETL + Y EAS V+ VG+ESTET KNIL+QIRETTS LNF+VIGRE+EV
Sbjct: 121 RMKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVL 180
Query: 181 NIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIIT 240
I+KLVIDSS+EH MSIIPIVGMGG+GKTTLAK VFNH+LIK HFD+TIWVCVS+PFII
Sbjct: 181 KIMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIV 240
Query: 241 NILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKE 300
NILE IF SLTNTS+G KE L LQKEMQGK YFLVLDDVWNEE +LW EL+DCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300
Query: 301 IAGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETI 360
IA KSGN IIVTTRS VAT M+T+ SH L KLSDD+CWSLFKESANANGLLMNPELE I
Sbjct: 301 IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360
Query: 361 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 420
R +VK+I G+PLVAKVLGG FEGN+ERWVT V+ +V+NI D+KDFVLS LKLSVD
Sbjct: 361 REVVVKRIGGIPLVAKVLGGTAKFEGNYERWVTTVESIVRNILNDEKDFVLSTLKLSVDS 420
Query: 421 LPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLL 480
LPLLSLKQCFA+CSNFPKD F KE+LIQMWIAQGF+Q +EGD+ ++ E+IGE YFNFLL
Sbjct: 421 LPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFNFLL 480
Query: 481 SRSLFEVV---IKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFK 540
SRSLF+ V I G ITHF MHDLIHD+AC +SN QK+E N SEK V LRTL+
Sbjct: 481 SRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRALRTLVINN 540
Query: 541 GYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDVESID 600
G + +N L+D LRVLV++ KN IGM KHLRYLD I G + ESI
Sbjct: 541 G-AIIHNKLEDSVYLRVLVLNS-DFIKNLPDSIGMLKHLRYLD---IIGSRIRRLPESIT 600
Query: 601 KLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSAYEASYE 660
LYNLQ LKL RI LPKNLRKLV LRHL FF I +KMPLH+SQLIHLQTL Y +E
Sbjct: 601 MLYNLQILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYIVGFE 660
Query: 661 QGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHW 720
+GRKIEELGPL +LKGSL+LLNL +VKSK EAI A+LIEK NL +L L HW
Sbjct: 661 KGRKIEELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTL----------HW 720
Query: 721 NNSSSGEG--NNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKI 780
N+SS GE NN++D+ +VLEGLQPH NLQSLSI + G PN +FVE LV I
Sbjct: 721 NSSSCGEEGYNNRNDS-------EVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAI 780
Query: 781 FLKDCRKCEMLPMLGQLPNLEALDISSMDNV----------------------------- 840
L++C KCEMLPMLGQLPNLE L +S M +V
Sbjct: 781 VLQECNKCEMLPMLGQLPNLEELRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFS 840
Query: 841 ----TKQWEDVSNGIAFPHLGILYIENCSKLMSVP-HCFVSLHTLFLEGCKELRELPNQL 900
+QWE++SNGI FPHL L + CSKL SVP H FVSL TL + GC+EL +LP++L
Sbjct: 841 HMEKLEQWEELSNGINFPHLETLSMFYCSKLTSVPDHEFVSLRTLRIVGCEELSKLPSKL 900
Query: 901 EGCNSIKCLKISDCPNLSVNVSNWSNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSE 960
E CN +KIS CP L +N+ N NL +EI G M++YD S +HQSS+T++ +TD S
Sbjct: 901 ECCN---FMKISGCPKLRLNMHNMCNLNELEINGPMEEYDFSFLMHQSSVTNIFLTD-SL 960
Query: 961 NNASQLPQQLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPSKEVM 1020
NA+QLPQQLQH+TTLK L I FNG+EALPEWL LA+LE++ +F CKNL+ PS++ M
Sbjct: 961 RNATQLPQQLQHLTTLKSLAIEYFNGVEALPEWLEKLANLETMGVFECKNLKRLPSQQAM 1020
BLAST of Lag0021704 vs. NCBI nr
Match:
XP_038895409.1 (disease resistance protein RGA2-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 665/1061 (62.68%), Postives = 776/1061 (73.14%), Query Frame = 0
Query: 3 EFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESV 62
EFLWTF VQE LKK+VK+AA++I VAWG +KEL KL+ WLLK+EAILRDI K+L++E V
Sbjct: 5 EFLWTFVVQEALKKVVKVAAQEIAVAWGFKKELEKLTKWLLKSEAILRDIITKKLYNEPV 64
Query: 63 RMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKL 122
RMWVE+LQH VYEADDLLDELVYEDLR+K ET+ ++KV FFS S N FLFR KM K++
Sbjct: 65 RMWVEDLQHFVYEADDLLDELVYEDLRRKAETEKIEKKVHNFFSCSHNPFLFRRKMAKRM 124
Query: 123 KEVTETLCQLYHEASSFGVVALVGQESTETNKNI-LKQIRETTSTLNFE--VIGREVEVS 182
K++TETL + Y EAS G+VALVG ESTE KNI LKQIR+TTS L E +IGREVEVS
Sbjct: 125 KDITETLYKHYCEASPLGLVALVGHESTEARKNIDLKQIRDTTSKLKPEEVIIGREVEVS 184
Query: 183 NIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIIT 242
NIVKLVI+SSNEHPMSIIPIVGMGG+GKTTLAK VFNH L++ HFD+T+WVCVSEPFII
Sbjct: 185 NIVKLVIESSNEHPMSIIPIVGMGGLGKTTLAKMVFNHGLVQTHFDKTLWVCVSEPFIII 244
Query: 243 NILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKE 302
NILE IF SLT TS+ +KENLL+ LQ+EM+G +YFLVLDDVWNEEA LW LKDCL E
Sbjct: 245 NILEGIFQSLTKTSSSCLNKENLLQRLQEEMRGNNYFLVLDDVWNEEAILWDNLKDCLNE 304
Query: 303 IAGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETI 362
IAGK GN IIVTTRS VAT M T SS HL KLSDD CWSLFK+ +A+GL ELE I
Sbjct: 305 IAGKPGNTIIVTTRSPDVATVMGTTSSIHLKKLSDDLCWSLFKKGVSADGL----ELEII 364
Query: 363 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 422
R E+V KI G+PLVAKVLGGAV FEGN+ERW+T+ K VVKNISKDDKDF+ SILKLSVD
Sbjct: 365 REEVVTKIGGIPLVAKVLGGAVKFEGNYERWMTKFKSVVKNISKDDKDFISSILKLSVDC 424
Query: 423 LPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLL 482
LPLLSLKQCFAYCSNFPKD+ F KESLIQ WIAQGF+QPQE D+E+M+EDIGE YF+ LL
Sbjct: 425 LPLLSLKQCFAYCSNFPKDYKFCKESLIQTWIAQGFIQPQEEDNELMMEDIGEGYFDLLL 484
Query: 483 SRSLFEVVIKG---LITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFK 542
SRSLFEVV + THF MHDLIHDIAC ISN +VES+L+NSSEKSV KLRT+I
Sbjct: 485 SRSLFEVVDEDSNERTTHFKMHDLIHDIACAISN--QVESNLHNSSEKSVRKLRTII--- 544
Query: 543 GYKVFN---NDLKDHACLRVLVVD-RIRCDKNFSCLIGMF-KHLRYLDISYISGYVELK- 602
+ + N + +D CLRVLV D + IG+ KHLRYL+I Y ELK
Sbjct: 545 -HDIDNSICDKFEDLVCLRVLVFALHPGWDVSLFNSIGIIAKHLRYLEIKQC--YHELKY 604
Query: 603 DVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSA 662
+ESI LYNLQTLKLG LP NLRKLVKLRHL+F I KMPLHM QLIHLQTL+A
Sbjct: 605 SLESIAMLYNLQTLKLGVGASLPTNLRKLVKLRHLEFKDIGDGKMPLHMRQLIHLQTLTA 664
Query: 663 YEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSS 722
+ E G KIEELGPLKNLKGSL L +LG+VKSK EA+GARLIEKKNL L WNSSS
Sbjct: 665 FFVG-EDGCKIEELGPLKNLKGSLDLGSLGEVKSKEEAMGARLIEKKNLTKLAFYWNSSS 724
Query: 723 KEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEK 782
+E ++ N +VLEGLQPH+NLQSLSI Y+GQ FPN+IFVE
Sbjct: 725 EEEDNLN------------------ELEVLEGLQPHNNLQSLSILHYNGQAFPNNIFVEG 784
Query: 783 LVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVT---KQWEDVSNG---IAFPHL--- 842
LV I L C KCEMLPMLGQLPNLE L IS +D+V +++ +N + FP L
Sbjct: 785 LVHIMLHGCMKCEMLPMLGQLPNLEELVISEVDSVKSIGREFYGSNNSNQRVCFPKLKKF 844
Query: 843 GILYIENCSKLMSVPH--CFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPNLSV 902
++Y+EN VP+ F L L++ GC +L +P+ E NSIK L CP L
Sbjct: 845 SLMYMENLENWEDVPNGMAFSHLTILYMRGCPKL-SIPHHFECLNSIKSLTHIHCPKLPQ 904
Query: 903 NVSN-WSNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSENN-ASQLPQQLQHITTLK 962
N+ N ++ LE +EI GCM+DYD SP IHQSSIT +S+TD SE+N A +LPQQL ++TTLK
Sbjct: 905 NMCNQYNKLEDMEIWGCMKDYDFSPLIHQSSITRVSLTDTSEDNPAIELPQQLGYLTTLK 964
Query: 963 VLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPSKEVMLCLTKLNKLHVEGCPLR 1022
+L I F GIEALP+WL NLA LE L+L+ CKNL+W PSK VML LTKLN++ V+GCPL
Sbjct: 965 LLEIFGFEGIEALPDWLENLACLEELKLYYCKNLKWLPSKHVMLSLTKLNRVEVKGCPL- 1024
Query: 1023 RHLCEGHSEWAKLSHLPKICVDVRLVPPLLMAPQATEPYTY 1039
L EG E AKLSH P V V V P P Y
Sbjct: 1025 LPLGEGDPERAKLSHFPNHFVKVVFVQSSWNCPYPFRPGYY 1032
BLAST of Lag0021704 vs. NCBI nr
Match:
XP_022145177.1 (putative disease resistance protein RGA3 [Momordica charantia])
HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 620/1027 (60.37%), Postives = 739/1027 (71.96%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLWTFAVQE L+K+VK+AA+QIG+AWGLEKELSKL+ WLLKAE IL DI+R++LHH+
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+W+E+LQ V+EA DLLDELVYEDLR+KVE KVR S S N F FR KM K
Sbjct: 61 SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEI-GKMNKVRGSISRSYNLFSFRRKMAK 120
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVSN 180
K+K+VTETL + Y EAS G LVG ES ETN+ +LKQIRETTS LNFEV+GRE+EVSN
Sbjct: 121 KMKDVTETLYKHYCEASPLG---LVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSN 180
Query: 181 IVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITN 240
IVKLVIDSSNEH SIIPIVGMGG+GKTTLA+ +FNH++IKGHFD+TIW+CVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIIN 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEI 300
ILE IF LT TS+G SKE L++ LQ+EM GK YFLVLDDVWNEE+ LW +L DCLK+I
Sbjct: 241 ILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQI 300
Query: 301 AGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIR 360
KSGN IIVTTRS++VAT +ET+SSHHL KLSDDQ W LFK SANANGL MNP+L+ IR
Sbjct: 301 TEKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDIIR 360
Query: 361 VELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRL 420
LVKKI GVPLVAKVLGGA+ FEG++E WV +VK VV+NISKDDKDFVLSILKLSVD L
Sbjct: 361 EILVKKIGGVPLVAKVLGGAIKFEGDYETWVKKVKSVVRNISKDDKDFVLSILKLSVDCL 420
Query: 421 PLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLS 480
PLLSLKQCFAYCSNF KD +FDKE+LIQMWIAQGF+Q EG +E EDIGE + NFLLS
Sbjct: 421 PLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNFLLS 480
Query: 481 RSLFEVVIKGL---ITHFTMHDLIHDIACDISNHQK-VESSLNNSSEKSVSKLRTLICFK 540
RSLF+ V+K + +THF MHDLIHDIAC ISNH K +E +N S KS KLRTLIC
Sbjct: 481 RSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLIC-- 540
Query: 541 GYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDVESID 600
+V N+L D LRVLV++ + D N IG KHLRYLDIS+ S V ESI
Sbjct: 541 NNQVIYNELMDCVSLRVLVLNSAKTD-NLLDSIGKLKHLRYLDISHYSIRVL---PESIA 600
Query: 601 KLYNLQTLKLGERIPLPKNLRKLVKLRHLQFF-CIKTKKMPLHMSQLIHLQTLSAYEASY 660
KLYNLQTLK+G LPKNLRK+V LRHL+F+ T +MP HMS LIHLQTL + A +
Sbjct: 601 KLYNLQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAGF 660
Query: 661 EQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNH 720
E G KIEELGPLKNLKG L+L NL +VKSK EAI A+L+EKKNL +L W+ +
Sbjct: 661 ENGCKIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDWSLDFE---- 720
Query: 721 WNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIF 780
N DD+ +VLEGLQPH NLQSL+I + GQV P +IFVE L I
Sbjct: 721 ---------RNDDDS-------EVLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAVIR 780
Query: 781 LKDCRKCEMLPMLGQLPNLEALDISSMDNV-TKQWEDVSNGI-----AFPHLGILYIENC 840
L +KCEMLPMLGQLPNL L+IS MD+V E NG F +G+ +E
Sbjct: 781 LCGGKKCEMLPMLGQLPNLVELEISFMDSVRCIGSEFYGNGSFSKLRKFDFIGLHNLEQW 840
Query: 841 SKLMSVPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPNLSVNVSNW----- 900
++ S+ + F SL TL + GC L++LPN LE C+S+ L + DCP L++NV N
Sbjct: 841 TEAASISNSFGSLQTLNVIGCGNLKKLPNGLEFCSSLHDLTVRDCPKLALNVQNMHNLSN 900
Query: 901 ----------------SNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSENNASQLPQ 960
+NL+ +E++GCMQDYD SPF+ S+ L + D S ++LPQ
Sbjct: 901 LFIHGFKELPLGLSHLTNLKEMEVVGCMQDYDFSPFLSLQSLIKLHLNDVS-RICTELPQ 960
Query: 961 QLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPSKEVMLCLTKLNK 996
+++T L+ L I F+ IEALPEWL NLASLE+L L CKNL+ PS+E ML LTKL
Sbjct: 961 HFENLTALETLTIVNFDAIEALPEWLENLASLETLNLEDCKNLKRLPSREAMLRLTKLEH 996
BLAST of Lag0021704 vs. NCBI nr
Match:
XP_038895415.1 (disease resistance protein RGA2-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1062.0 bits (2745), Expect = 3.4e-306
Identity = 630/1049 (60.06%), Postives = 724/1049 (69.02%), Query Frame = 0
Query: 3 EFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESV 62
EFLWTF VQE LKK+VK+AA++I VAWG +KEL KL+ WLLK+EAILRDI K+L++E V
Sbjct: 5 EFLWTFVVQEALKKVVKVAAQEIAVAWGFKKELEKLTKWLLKSEAILRDIITKKLYNEPV 64
Query: 63 RMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKL 122
RMWVE+LQH VYEADDLLDELVYEDLR+K ET+ ++KV FFS S N FLFR KM K++
Sbjct: 65 RMWVEDLQHFVYEADDLLDELVYEDLRRKAETEKIEKKVHNFFSCSHNPFLFRRKMAKRM 124
Query: 123 KEVTETLCQLYHEASSFGVVALVGQESTETNKNI-LKQIRETTSTLNFE--VIGREVEVS 182
K++TETL + Y EAS G+VALVG ESTE KNI LKQIR+TTS L E +IGREVEVS
Sbjct: 125 KDITETLYKHYCEASPLGLVALVGHESTEARKNIDLKQIRDTTSKLKPEEVIIGREVEVS 184
Query: 183 NIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIIT 242
NIVKLVI+SSNEHPMSIIPIVGMGG+GKTTLAK VFNH L++ HFD+T+WVCVSEPFII
Sbjct: 185 NIVKLVIESSNEHPMSIIPIVGMGGLGKTTLAKMVFNHGLVQTHFDKTLWVCVSEPFIII 244
Query: 243 NILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKE 302
NILE IF SLT TS+ +KENLL+ LQ+EM+G +YFLVLDDVWNEEA LW LKDCL E
Sbjct: 245 NILEGIFQSLTKTSSSCLNKENLLQRLQEEMRGNNYFLVLDDVWNEEAILWDNLKDCLNE 304
Query: 303 IAGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETI 362
IAGK GN IIVTTRS VAT M T SS HL KLSDD CWSLFK+ +A+GL ELE I
Sbjct: 305 IAGKPGNTIIVTTRSPDVATVMGTTSSIHLKKLSDDLCWSLFKKGVSADGL----ELEII 364
Query: 363 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 422
R E+V KI G+PLVAKVLGGAV FEGN+ERW+T+ K VVKNISKDDKDF+ SILKLSVD
Sbjct: 365 REEVVTKIGGIPLVAKVLGGAVKFEGNYERWMTKFKSVVKNISKDDKDFISSILKLSVDC 424
Query: 423 LPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLL 482
LPLLSLKQCFAYCSNFPKD+ F KESLIQ WIAQGF+QPQE D+E+M+EDIGE YF+ LL
Sbjct: 425 LPLLSLKQCFAYCSNFPKDYKFCKESLIQTWIAQGFIQPQEEDNELMMEDIGEGYFDLLL 484
Query: 483 SRSLFEVVIKG---LITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFK 542
SRSLFEVV + THF MHDLIHDIAC ISN +VES+L+NSSEKSV KLRT+I
Sbjct: 485 SRSLFEVVDEDSNERTTHFKMHDLIHDIACAISN--QVESNLHNSSEKSVRKLRTII--- 544
Query: 543 GYKVFN---NDLKDHACLRVLVVD-RIRCDKNFSCLIGMF-KHLRYLDISYISGYVELK- 602
+ + N + +D CLRVLV D + IG+ KHLRYL+I Y ELK
Sbjct: 545 -HDIDNSICDKFEDLVCLRVLVFALHPGWDVSLFNSIGIIAKHLRYLEIKQC--YHELKY 604
Query: 603 DVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSA 662
+ESI LYNLQTLKLG LP NLRKLVKLRHL+F I KMPLHM QLIHLQTL+A
Sbjct: 605 SLESIAMLYNLQTLKLGVGASLPTNLRKLVKLRHLEFKDIGDGKMPLHMRQLIHLQTLTA 664
Query: 663 YEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSS 722
+ E G KIEELGPLKNLKGSL L +LG+VKSK EA+GARLIEKKNL L WNSSS
Sbjct: 665 FFVG-EDGCKIEELGPLKNLKGSLDLGSLGEVKSKEEAMGARLIEKKNLTKLAFYWNSSS 724
Query: 723 KEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEK 782
+E ++ N +VLEGLQPH+NLQSLSI Y+GQ FPN+IFVE
Sbjct: 725 EEEDNLN------------------ELEVLEGLQPHNNLQSLSILHYNGQAFPNNIFVEG 784
Query: 783 LVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHLGILYIENCS 842
LV I L C KCEMLPMLGQLPNLE L IS +D+V S G F Y N S
Sbjct: 785 LVHIMLHGCMKCEMLPMLGQLPNLEELVISEVDSVK------SIGREF------YGSNNS 844
Query: 843 KLMSVPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPNLSVNVSNWSNLEAV 902
CF L L + N+ NW ++
Sbjct: 845 NQRV---CFPKLKKFSLMYME----------------------------NLENWEDVP-- 904
Query: 903 EIIGCMQDYDISPFIHQSSITHLSMTDNSENN-ASQLPQQLQHITTLKVLRICLFNGIEA 962
D SE+N A +LPQQL ++TTLK+L I F GIEA
Sbjct: 905 -------------------------NDTSEDNPAIELPQQLGYLTTLKLLEIFGFEGIEA 951
Query: 963 LPEWLGNLASLESLQLFACKNLEWFPSKEVMLCLTKLNKLHVEGCPLRRHLCEGHSEWAK 1022
LP+WL NLA LE L+L+ CKNL+W PSK VML LTKLN++ V+GCPL L EG E AK
Sbjct: 965 LPDWLENLACLEELKLYYCKNLKWLPSKHVMLSLTKLNRVEVKGCPL-LPLGEGDPERAK 951
Query: 1023 LSHLPKICVDVRLVPPLLMAPQATEPYTY 1039
LSH P V V V P P Y
Sbjct: 1025 LSHFPNHFVKVVFVQSSWNCPYPFRPGYY 951
BLAST of Lag0021704 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 426.4 bits (1095), Expect = 9.4e-118
Identity = 341/1058 (32.23%), Postives = 512/1058 (48.39%), Query Frame = 0
Query: 10 VQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENL 69
+Q L L ++G+ +G EKE KLS+ +A+L D K+L +++++ W++ L
Sbjct: 6 LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+LD+ K E K+ V + P + F YK+ K++KE+ E L
Sbjct: 66 NVAAYEVDDILDDC-------KTEAARFKQAVLGRYHP--RTITFCYKVGKRMKEMMEKL 125
Query: 130 CQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNF-EVIGREVEVSNIVKLVIDS 189
+ E +F + + + R+T L +V GRE E IVK++I++
Sbjct: 126 DAIAEERRNFHLDERIIERQAAR--------RQTGFVLTEPKVYGREKEEDEIVKILINN 185
Query: 190 -SNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFH 249
S + ++PI+GMGG+GKTTLA+ VFN + I HF+ IWVCVS+ F +++ I
Sbjct: 186 VSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVE 245
Query: 250 SLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGNV 309
S+ S G L K LQ+ + GK YFLVLDDVWNE+ W L+ LK G SG
Sbjct: 246 SIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGAS 305
Query: 310 IIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIRVELVKKI 369
I++TTR K+ + M TL + L+ LS + CW LFK+ A + +P+L I E+VKK
Sbjct: 306 ILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKC 365
Query: 370 DGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQ 429
GVPL AK LGG + F+ W + N+ +D+ VL L+LS LP L L+Q
Sbjct: 366 GGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENS-VLPALRLSYHHLP-LDLRQ 425
Query: 430 CFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLSRSLF-EV 489
CFAYC+ FPKD +KE LI +W+A F+ + M +ED+G E +N L RS F E+
Sbjct: 426 CFAYCAVFPKDTKIEKEYLIALWMAHSFLLSK---GNMELEDVGNEVWNELYLRSFFQEI 485
Query: 490 VIKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKS-------VSKLRTLICFKGYK 549
+K T+F MHDLIHD+A + + S+ + K V+ + ++ +
Sbjct: 486 EVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGFSE 545
Query: 550 VFNND----LKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDI------SYISGYVEL 609
V ++ K LRVL + ++ +G HLRYLD+ S +L
Sbjct: 546 VVSSYSPSLFKRFVSLRVLNLSNSEFEQ-LPSSVGDLVHLRYLDLSGNKICSLPKRLCKL 605
Query: 610 KDVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLS 669
++++++D LYN Q+L LPK KL LR+L MP + L L+TL
Sbjct: 606 QNLQTLD-LYNCQSLSC-----LPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTLG 665
Query: 670 AYEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSS 729
+ +G ++ EL L NL+G++ + +L +VK+ EA A L K NL L ++W+
Sbjct: 666 YFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDR- 725
Query: 730 SKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFP---NSI 789
N ++ KVLE L+PH NL+ L I + G P N
Sbjct: 726 -------------------PNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHS 785
Query: 790 FVEKLVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHLGILYI 849
++ +V I + C C LP G+LP LE+L++ +D S + + Y+
Sbjct: 786 VLKNVVSILISGCENCSCLPPFGELPCLESLEL----------QDGS-------VEVEYV 845
Query: 850 ENCSKLMSVPHCFVSLHTLFLEGCKELRELPNQ--LEGCNSIKCLKISDCPNLSVNVSNW 909
E+ L F SL L + G L+ L E ++ +KISDCP
Sbjct: 846 EDSGFL--TRRRFPSLRKLHIGGFCNLKGLQRMKGAEQFPVLEEMKISDCPMFV--FPTL 905
Query: 910 SNLEAVEIIGCMQDYDISPFIHQSSITHL-------------SMTDNSEN---------- 969
S+++ +EI G +S + S++T L M N EN
Sbjct: 906 SSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLE 965
Query: 970 NASQLPQQLQHITTLKVLRICLFNGIEALP-EWLGNLASLESLQLFACKNLEWFPSKEVM 1017
N +LP L + LK L I +E+LP E L L+SL L + C L+ P E +
Sbjct: 966 NLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLP--EGL 988
BLAST of Lag0021704 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 420.2 bits (1079), Expect = 6.7e-116
Identity = 341/1037 (32.88%), Postives = 518/1037 (49.95%), Query Frame = 0
Query: 10 VQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENL 69
+Q L L ++ + +G + E +LS+ +A+L D K+L+++ + W++ L
Sbjct: 6 IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+LDE +T+ T+ ++ FR+K+ K++ +V + L
Sbjct: 66 NAATYEVDDILDE---------YKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKL 125
Query: 130 CQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNF-EVIGREVEVSNIVKLVIDS 189
+ E +F + + + RET S L +V GR+ E IVK++I++
Sbjct: 126 KAIAEERKNFHLHEKIVERQAVR--------RETGSVLTEPQVYGRDKEKDEIVKILINN 185
Query: 190 -SNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFH 249
S+ +S++PI+GMGG+GKTTLA+ VFN + + HF IW+CVSE F +++ I
Sbjct: 186 VSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVE 245
Query: 250 SLTNTS-NGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGN 309
S+ G L K LQ+ + GK Y LVLDDVWNE+ W L+ LK G SG
Sbjct: 246 SIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGA 305
Query: 310 VIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIRVELVKK 369
++ TTR KV + M TL + L+ LS + CW LF + A + +NP L I E+VKK
Sbjct: 306 SVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKK 365
Query: 370 IDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLK 429
GVPL AK LGG + F+ W + N+ +D+ +L L+LS +LP L LK
Sbjct: 366 SGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESS-ILPALRLSYHQLP-LDLK 425
Query: 430 QCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLSRSLF-E 489
QCFAYC+ FPKD +KE LI +W+A GF+ + M +ED+G+E + L RS F E
Sbjct: 426 QCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSK---GNMELEDVGDEVWKELYLRSFFQE 485
Query: 490 VVIKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFKGYKVFNN-- 549
+ +K T+F MHDLIHD+A + + S++ ++ S + + + I F F
Sbjct: 486 IEVKDGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMS-IGFAEVVFFYTLP 545
Query: 550 DLKDHACLRVLVVDRIRCDKNFSCL---IGMFKHLRYLDISYISGYVELKDVESIDKLYN 609
L+ LRVL + D F+ L IG HLRYL++ Y SG L + + KL N
Sbjct: 546 PLEKFISLRVLNLG----DSTFNKLPSSIGDLVHLRYLNL-YGSGMRSLP--KQLCKLQN 605
Query: 610 LQTLKL---GERIPLPKNLRKLVKLRHLQFFCIKTKK-MPLHMSQLIHLQTLSAYEASYE 669
LQTL L + LPK KL LR+L ++ MP + L L+TL + +
Sbjct: 606 LQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRK 665
Query: 670 QGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHW 729
+G ++ ELG L NL GS+K+ +L +VK+ +A A L K NL L ++W
Sbjct: 666 KGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSW---------- 725
Query: 730 NNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFP---NSIFVEKLVK 789
NN + ++ KVLE L+PH NL SL I + G P N ++ +V
Sbjct: 726 --------NNFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVS 785
Query: 790 IFLKDCRKCEMLPMLGQLPNLEALDI----SSMDNVTKQWEDVSNG----IAFPHLGILY 849
I + + R C LP G LP LE+L++ + ++ V + DV +G I FP L L
Sbjct: 786 ILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLD 845
Query: 850 IENCSKLMSVPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPNLSVNVSNWS 909
I + L + L EG ++ L + I +CP L+++ S
Sbjct: 846 IWDFGSLKGL---------LKKEGEEQFPVLEEMI----------IHECPFLTLS----S 905
Query: 910 NLEAVEIIGCMQDYDISPFIHQ--SSITHLS-MTDNSENNASQLPQQLQHITTLKVLRIC 969
NL A+ + + + F + ++ +L +T + NN +LP L + LK L+I
Sbjct: 906 NLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQ 965
Query: 970 LFNGIEALP-EWLGNLASLESLQLFACKNLEWFPSKEVMLCLTKLNKLHVEGCPLRRHLC 1017
L +E+LP E L L+SL L + C L+ P E + LT L L + GCP C
Sbjct: 966 LCCALESLPEEGLEGLSSLTELFVEHCNMLKCLP--EGLQHLTTLTSLKIRGCPQLIKRC 966
BLAST of Lag0021704 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 407.9 bits (1047), Expect = 3.5e-112
Identity = 319/1017 (31.37%), Postives = 499/1017 (49.07%), Query Frame = 0
Query: 10 VQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENL 69
+Q L L ++ + +G + E +LS+ +A+L D K+L+ + + W++ L
Sbjct: 6 IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+LDE +T+ T+ ++ FR+K+ K++ +V + L
Sbjct: 66 NAATYEVDDILDE---------YKTKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVMKKL 125
Query: 130 CQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNF-EVIGREVEVSNIVKLVID- 189
+ E F + + + T RET S L +V GR+ E IVK++I+
Sbjct: 126 NAIAEERKKFHLQEKIIERQAAT--------RETGSVLTEPQVYGRDKEKDEIVKILINT 185
Query: 190 SSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFH 249
+S+ +S++PI+GMGG+GKTTL++ VFN + + F IW+C+S+ F +++ I
Sbjct: 186 ASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVE 245
Query: 250 SLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGNV 309
S+ S L K LQ+ + GK YFLVLDDVWNE+ W L+ LK G SG
Sbjct: 246 SIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAF 305
Query: 310 IIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIRVELVKKI 369
++ TTR KV + M TL + L+ LS + CW LF + A + +NP L I E+VKK
Sbjct: 306 VLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKC 365
Query: 370 DGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQ 429
GVPL AK LGG + F+ W + N+ +D+ +L L+LS LP L L+Q
Sbjct: 366 GGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESS-ILPALRLSYHHLP-LDLRQ 425
Query: 430 CFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLSRSLF-EV 489
CF YC+ FPKD KE+LI W+A GF+ + + +ED+G E +N L RS F E+
Sbjct: 426 CFVYCAVFPKDTKMAKENLIAFWMAHGFLLSK---GNLELEDVGNEVWNELYLRSFFQEI 485
Query: 490 VIKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFKGYKVFNND-L 549
++ T+F MHDLIHD+A + + S++ + + ++ + ++ L
Sbjct: 486 EVESGKTYFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYSPSLL 545
Query: 550 KDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDV-ESIDKLYNLQTL 609
+ LRVL + ++ IG HLRYLD +SG ++++ + + KL NLQTL
Sbjct: 546 QKFVSLRVLNLRNSNLNQ-LPSSIGDLVHLRYLD---LSGNFRIRNLPKRLCKLQNLQTL 605
Query: 610 KL---GERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSAYEASYEQGRKI 669
L LPK KL LR+L P + L L++LS + +G ++
Sbjct: 606 DLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQL 665
Query: 670 EELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHWNNSSS 729
EL L NL GS+ + L +VK +A A L K NL L L+W+ +G H +S
Sbjct: 666 GELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWD---LDGKHRYDS-- 725
Query: 730 GEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFP---NSIFVEKLVKIFLKD 789
+VLE L+PH NL+ L IN + G P N ++ +V I ++
Sbjct: 726 ----------------EVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRG 785
Query: 790 CRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHLGILYIENCSKLMSVPHC 849
C C LP G+LP LE+L++ + + ED + FP L L I + S L +
Sbjct: 786 CENCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGRFPSLRKLVIWDFSNLKGL--- 845
Query: 850 FVSLHTLFLEGCKELRELPNQ-LEGCNSIKCLKISDCPNLSVNVSNWSNLEAVEIIGCMQ 909
L +EG K+ L C +S L V V++ + L ++
Sbjct: 846 ------LKMEGEKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSIS------ 905
Query: 910 DYDISPFIHQSSITHLSMTDNSENNASQLPQQL-QHITTLKVLRICLFNGIEALPEWLGN 969
+ ++T L ++DN E A+ LP+++ + + LK L+I F ++ LP L +
Sbjct: 906 --------NLRALTSLDISDNVE--ATSLPEEMFKSLANLKYLKISFFRNLKELPTSLAS 946
Query: 970 LASLESLQLFACKNLEWFPSKEVMLCLTKLNKLHVEGCPLRRHLCEGHSEWAKLSHL 1014
L +L+SL+ C LE P +E + LT L +L V C + + L EG L+ L
Sbjct: 966 LNALKSLKFEFCDALESLP-EEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTL 946
BLAST of Lag0021704 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 404.4 bits (1038), Expect = 3.8e-111
Identity = 330/1051 (31.40%), Postives = 511/1051 (48.62%), Query Frame = 0
Query: 10 VQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENL 69
+Q L+ L +++ + +G EKE KLS+ +A+L+D K+L +++ W++ L
Sbjct: 6 LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+L E E +R ++ F+ P FR+K+ +++KE+ E L
Sbjct: 66 NSAAYEVDDILGECKNEAIR-------FEQSRLGFYHP--GIINFRHKIGRRMKEIMEKL 125
Query: 130 CQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNF-EVIGREVEVSNIVKLVIDS 189
+ E F + + T + RET L +V GR+ E IVK++I++
Sbjct: 126 DAISEERRKFHFLEKI------TERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINN 185
Query: 190 SN-EHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFH 249
N + + PI+GMGG+GKTTLA+ +FN + + HF+ IWVCVS+ F +++ I
Sbjct: 186 VNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIG 245
Query: 250 SLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGNV 309
++ +S + + K LQ+ + GK Y LVLDDVWN++ W +L+ L G G
Sbjct: 246 NIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGAS 305
Query: 310 IIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIRVELVKKI 369
I+ TTR KV + M TL +HL+ LS LF + A NP L I E+VKK
Sbjct: 306 ILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKC 365
Query: 370 DGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQ 429
GVPL AK LGG + F+ W + ++ +D+ +L L+LS LP L L+Q
Sbjct: 366 GGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESS-ILPALRLSYHHLP-LDLRQ 425
Query: 430 CFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLSRSLF-EV 489
CFAYC+ FPKD KE+LI +W+A GF+ + + +ED+G E +N L RS F E+
Sbjct: 426 CFAYCAVFPKDTKMIKENLITLWMAHGFLLSK---GNLELEDVGNEVWNELYLRSFFQEI 485
Query: 490 VIKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFKGYKVFNND-- 549
K T+F +HDLIHD+A + + +S N E +V + + V ++
Sbjct: 486 EAKSGNTYFKIHDLIHDLATSLFS---ASASCGNIREINVKDYKHTVSIGFAAVVSSYSP 545
Query: 550 --LKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDVESIDKLYNLQ 609
LK LRVL + + ++ IG HLRYLD+S + + L E + KL NLQ
Sbjct: 546 SLLKKFVSLRVLNLSYSKLEQ-LPSSIGDLLHLRYLDLS-CNNFRSLP--ERLCKLQNLQ 605
Query: 610 TLKLGERIP---LPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSAYEASYEQGR 669
TL + LPK KL LRHL P + L L+TL + ++G
Sbjct: 606 TLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKKGY 665
Query: 670 KIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHWNNS 729
++ EL L NL GS+ + +L +VK+ +A A L K NL L ++W
Sbjct: 666 QLGELKNL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSW------------- 725
Query: 730 SSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFP---NSIFVEKLVKIFL 789
+N N ++ KVLE L+PH NL+ L I + G FP N +EK++ + +
Sbjct: 726 -----DNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRI 785
Query: 790 KDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHLGILYIE--NCSKLMS 849
K C+ C LP G+LP LE L++ NG A + Y+E + S
Sbjct: 786 KSCKNCLCLPPFGELPCLENLEL-------------QNGSA----EVEYVEEDDVHSRFS 845
Query: 850 VPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLK---ISDCPNLSVNVSNWSNLEAVE 909
F SL L + + L+ L + EG L+ I CP L V S+++ +E
Sbjct: 846 TRRSFPSLKKLRIWFFRSLKGLMKE-EGEEKFPMLEEMAILYCP-LFV-FPTLSSVKKLE 905
Query: 910 IIGCMQDYDISPFIHQSSITHLSMTDNSENNASQLPQQL-QHITTLKVLRICLFNGIEAL 969
+ G +S + S++T L + N A+ LP+++ +T L+ L F ++ L
Sbjct: 906 VHGNTNTRGLSSISNLSTLTSLRIGAN--YRATSLPEEMFTSLTNLEFLSFFDFKNLKDL 965
Query: 970 PEWLGNLASLESLQLFACKNLEWFPSK----------------EVMLC-------LTKLN 1017
P L +L +L+ LQ+ +C +LE FP + +++ C LT L
Sbjct: 966 PTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALT 985
BLAST of Lag0021704 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 341.7 bits (875), Expect = 3.0e-92
Identity = 294/1002 (29.34%), Postives = 478/1002 (47.70%), Query Frame = 0
Query: 32 EKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENLQHAVYEADDLLDELVYEDLRQK 91
E L +LS LL A+L D + K++ + V WV L+ VY A+D LD++ E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 92 V----ETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLYHEASSFGVVALVGQ 151
+ + N ++R S D + +L++VT L +L + + G+ L
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155
Query: 152 ESTETNKNILKQIRETTSTLN-FEVIGREVEVSNIVKLVI-DSSNEHPMSIIPIVGMGGV 211
I KQ TTS ++ EV GR+ + I++ +I ++ ++ ++++ IVG+GGV
Sbjct: 156 --------IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 215
Query: 212 GKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFHSLTNTSNGFKSKENLLKG 271
GKTTL++ ++N + ++ +F +W VSE F + I ++++ S+T+ F + L
Sbjct: 216 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 275
Query: 272 LQKEMQGKS--YFLVLDDVWNEEASLWGELKDCLKEIAGKSGNVIIVTTRSVKVATFMET 331
L++ + G + LVLDD+WNE + W L+ I G+ I+VTTRS +VA+ M
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRVASIMCA 335
Query: 332 LSSHHLNKLSDDQCWSLFKESANAN-GLLMNPELETIRVELVKKIDGVPLVAKVLGGAVT 391
+ H+L LSD CWSLF ++ N +N E+ + +V K G+PL K LGG +
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 392 FEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFD 451
FEG W + + ++ DK +L +L++S LP LK+CFAYCS FPK H F+
Sbjct: 396 FEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 455
Query: 452 KESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLSRSLFEVVIKGLITHFTMHDLIH 511
K+ ++ +W+A+GF+Q +E++G EYF+ L SRSL ++ T + MHD I+
Sbjct: 456 KDKVVLLWMAEGFLQQTRSSKN--LEELGNEYFSELESRSL----LQKTKTRYIMHDFIN 515
Query: 512 DIA----------------CDISNHQKVESSLNNS--------SEKSVSKLRTLI----- 571
++A +S + S L ++ + + V LRT +
Sbjct: 516 ELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLT 575
Query: 572 -----CFKGYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVE 631
C V L LRVL + + + H R+LD+S E
Sbjct: 576 NSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLS----RTE 635
Query: 632 LKDV-ESIDKLYNLQTLKL---GERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIH 691
L+ + +S+ +YNLQTL L LP ++ L+ LR+L K ++MP +L
Sbjct: 636 LEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKS 695
Query: 692 LQTLSAYEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVL 751
LQTL+ + S G +I ELG L +L G LK++ L +V A+A A L KK+L ++
Sbjct: 696 LQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDF 755
Query: 752 TWNSSSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPN 811
W + S NN++ N+ + V E L+PH +++ L+I Y G+ FP+
Sbjct: 756 VWRTGSSSSE--NNTNPHRTQNEAE---------VFEKLRPHRHIEKLAIERYKGRRFPD 815
Query: 812 SI---FVEKLVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHL 871
+ ++V I L++C+ C LP LGQLP L+ L IS M + S G F
Sbjct: 816 WLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQ------SIGRKF--- 875
Query: 872 GILYIENCSKLMSVPHCFVSLHTLFLEGCKELRE----LPNQLEGCNSIKCLKISDCPNL 931
Y + F SL TL + + +E + + S+K L I CP L
Sbjct: 876 ---YFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPEL 935
Query: 932 SVNVSNW-SNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSENNASQLPQQLQHITTL 976
+ + + +L ++ I C D P H+ S +L + + + L H L
Sbjct: 936 TGTLPTFLPSLISLHIYKCGL-LDFQPDHHEYSYRNLQTLSIKSSCDTLVKFPLNHFANL 991
BLAST of Lag0021704 vs. ExPASy TrEMBL
Match:
A0A6J1CUL8 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111014734 PE=4 SV=1)
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 668/1059 (63.08%), Postives = 781/1059 (73.75%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLWTFAVQE LKK++KLAAEQIGVAW L+KELSKL LLKAE IL DI+R++L HE
Sbjct: 1 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+WVE+LQH VY+ADDLLD+LVYEDLRQKVE + KVR F SPS+N FLFR+KM K
Sbjct: 61 SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKI-NKVRNFLSPSNNPFLFRFKMAK 120
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETN-KNILKQIRETTSTLNFEVIGREVEVS 180
++K ++ETL + Y EAS V+ VG+ESTET KNIL+QIRETTS LNF+VIGRE+EV
Sbjct: 121 RMKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVL 180
Query: 181 NIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIIT 240
I+KLVIDSS+EH MSIIPIVGMGG+GKTTLAK VFNH+LIK HFD+TIWVCVS+PFII
Sbjct: 181 KIMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIV 240
Query: 241 NILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKE 300
NILE IF SLTNTS+G KE L LQKEMQGK YFLVLDDVWNEE +LW EL+DCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300
Query: 301 IAGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETI 360
IA KSGN IIVTTRS VAT M+T+ SH L KLSDD+CWSLFKESANANGLLMNPELE I
Sbjct: 301 IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360
Query: 361 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 420
R +VK+I G+PLVAKVLGG FEGN+ERWVT V+ +V+NI D+KDFVLS LKLSVD
Sbjct: 361 REVVVKRIGGIPLVAKVLGGTAKFEGNYERWVTTVESIVRNILNDEKDFVLSTLKLSVDS 420
Query: 421 LPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLL 480
LPLLSLKQCFA+CSNFPKD F KE+LIQMWIAQGF+Q +EGD+ ++ E+IGE YFNFLL
Sbjct: 421 LPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFNFLL 480
Query: 481 SRSLFEVV---IKGLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFK 540
SRSLF+ V I G ITHF MHDLIHD+AC +SN QK+E N SEK V LRTL+
Sbjct: 481 SRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRALRTLVINN 540
Query: 541 GYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDVESID 600
G + +N L+D LRVLV++ KN IGM KHLRYLD I G + ESI
Sbjct: 541 G-AIIHNKLEDSVYLRVLVLNS-DFIKNLPDSIGMLKHLRYLD---IIGSRIRRLPESIT 600
Query: 601 KLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTLSAYEASYE 660
LYNLQ LKL RI LPKNLRKLV LRHL FF I +KMPLH+SQLIHLQTL Y +E
Sbjct: 601 MLYNLQILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYIVGFE 660
Query: 661 QGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHW 720
+GRKIEELGPL +LKGSL+LLNL +VKSK EAI A+LIEK NL +L L HW
Sbjct: 661 KGRKIEELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTL----------HW 720
Query: 721 NNSSSGEG--NNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKI 780
N+SS GE NN++D+ +VLEGLQPH NLQSLSI + G PN +FVE LV I
Sbjct: 721 NSSSCGEEGYNNRNDS-------EVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAI 780
Query: 781 FLKDCRKCEMLPMLGQLPNLEALDISSMDNV----------------------------- 840
L++C KCEMLPMLGQLPNLE L +S M +V
Sbjct: 781 VLQECNKCEMLPMLGQLPNLEELRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFS 840
Query: 841 ----TKQWEDVSNGIAFPHLGILYIENCSKLMSVP-HCFVSLHTLFLEGCKELRELPNQL 900
+QWE++SNGI FPHL L + CSKL SVP H FVSL TL + GC+EL +LP++L
Sbjct: 841 HMEKLEQWEELSNGINFPHLETLSMFYCSKLTSVPDHEFVSLRTLRIVGCEELSKLPSKL 900
Query: 901 EGCNSIKCLKISDCPNLSVNVSNWSNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSE 960
E CN +KIS CP L +N+ N NL +EI G M++YD S +HQSS+T++ +TD S
Sbjct: 901 ECCN---FMKISGCPKLRLNMHNMCNLNELEINGPMEEYDFSFLMHQSSVTNIFLTD-SL 960
Query: 961 NNASQLPQQLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPSKEVM 1020
NA+QLPQQLQH+TTLK L I FNG+EALPEWL LA+LE++ +F CKNL+ PS++ M
Sbjct: 961 RNATQLPQQLQHLTTLKSLAIEYFNGVEALPEWLEKLANLETMGVFECKNLKRLPSQQAM 1020
BLAST of Lag0021704 vs. ExPASy TrEMBL
Match:
A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)
HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 620/1027 (60.37%), Postives = 739/1027 (71.96%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLWTFAVQE L+K+VK+AA+QIG+AWGLEKELSKL+ WLLKAE IL DI+R++LHH+
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+W+E+LQ V+EA DLLDELVYEDLR+KVE KVR S S N F FR KM K
Sbjct: 61 SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEI-GKMNKVRGSISRSYNLFSFRRKMAK 120
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVSN 180
K+K+VTETL + Y EAS G LVG ES ETN+ +LKQIRETTS LNFEV+GRE+EVSN
Sbjct: 121 KMKDVTETLYKHYCEASPLG---LVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSN 180
Query: 181 IVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITN 240
IVKLVIDSSNEH SIIPIVGMGG+GKTTLA+ +FNH++IKGHFD+TIW+CVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIIN 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEI 300
ILE IF LT TS+G SKE L++ LQ+EM GK YFLVLDDVWNEE+ LW +L DCLK+I
Sbjct: 241 ILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQI 300
Query: 301 AGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIR 360
KSGN IIVTTRS++VAT +ET+SSHHL KLSDDQ W LFK SANANGL MNP+L+ IR
Sbjct: 301 TEKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDIIR 360
Query: 361 VELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRL 420
LVKKI GVPLVAKVLGGA+ FEG++E WV +VK VV+NISKDDKDFVLSILKLSVD L
Sbjct: 361 EILVKKIGGVPLVAKVLGGAIKFEGDYETWVKKVKSVVRNISKDDKDFVLSILKLSVDCL 420
Query: 421 PLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLS 480
PLLSLKQCFAYCSNF KD +FDKE+LIQMWIAQGF+Q EG +E EDIGE + NFLLS
Sbjct: 421 PLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNFLLS 480
Query: 481 RSLFEVVIKGL---ITHFTMHDLIHDIACDISNHQK-VESSLNNSSEKSVSKLRTLICFK 540
RSLF+ V+K + +THF MHDLIHDIAC ISNH K +E +N S KS KLRTLIC
Sbjct: 481 RSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLIC-- 540
Query: 541 GYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDVESID 600
+V N+L D LRVLV++ + D N IG KHLRYLDIS+ S V ESI
Sbjct: 541 NNQVIYNELMDCVSLRVLVLNSAKTD-NLLDSIGKLKHLRYLDISHYSIRVL---PESIA 600
Query: 601 KLYNLQTLKLGERIPLPKNLRKLVKLRHLQFF-CIKTKKMPLHMSQLIHLQTLSAYEASY 660
KLYNLQTLK+G LPKNLRK+V LRHL+F+ T +MP HMS LIHLQTL + A +
Sbjct: 601 KLYNLQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAGF 660
Query: 661 EQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNH 720
E G KIEELGPLKNLKG L+L NL +VKSK EAI A+L+EKKNL +L W+ +
Sbjct: 661 ENGCKIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDWSLDFE---- 720
Query: 721 WNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIF 780
N DD+ +VLEGLQPH NLQSL+I + GQV P +IFVE L I
Sbjct: 721 ---------RNDDDS-------EVLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAVIR 780
Query: 781 LKDCRKCEMLPMLGQLPNLEALDISSMDNV-TKQWEDVSNGI-----AFPHLGILYIENC 840
L +KCEMLPMLGQLPNL L+IS MD+V E NG F +G+ +E
Sbjct: 781 LCGGKKCEMLPMLGQLPNLVELEISFMDSVRCIGSEFYGNGSFSKLRKFDFIGLHNLEQW 840
Query: 841 SKLMSVPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPNLSVNVSNW----- 900
++ S+ + F SL TL + GC L++LPN LE C+S+ L + DCP L++NV N
Sbjct: 841 TEAASISNSFGSLQTLNVIGCGNLKKLPNGLEFCSSLHDLTVRDCPKLALNVQNMHNLSN 900
Query: 901 ----------------SNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSENNASQLPQ 960
+NL+ +E++GCMQDYD SPF+ S+ L + D S ++LPQ
Sbjct: 901 LFIHGFKELPLGLSHLTNLKEMEVVGCMQDYDFSPFLSLQSLIKLHLNDVS-RICTELPQ 960
Query: 961 QLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPSKEVMLCLTKLNK 996
+++T L+ L I F+ IEALPEWL NLASLE+L L CKNL+ PS+E ML LTKL
Sbjct: 961 HFENLTALETLTIVNFDAIEALPEWLENLASLETLNLEDCKNLKRLPSREAMLRLTKLEH 996
BLAST of Lag0021704 vs. ExPASy TrEMBL
Match:
A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)
HSP 1 Score: 1057.0 bits (2732), Expect = 5.2e-305
Identity = 610/1042 (58.54%), Postives = 728/1042 (69.87%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLWTFAVQE L+K+VK+AA+QIG+A GLEKELS L+ WL KAE ILRDI+RK+ H +
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSHSD 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+WVE+LQ V+EADDLLDELVYE LR+KVE KVR FS S N FLFR KM K
Sbjct: 61 SVRLWVEDLQDVVHEADDLLDELVYEHLRRKVEIGKI-NKVRDSFSTSHNVFLFRLKMAK 120
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVSN 180
K+K++T+ L + Y EAS G LVGQESTE N+ +L QIR+TTS LNFEV+GRE EVSN
Sbjct: 121 KIKDITQILHKHYCEASPLG---LVGQESTEANQIVLSQIRDTTSNLNFEVVGREDEVSN 180
Query: 181 IVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITN 240
IVKLVIDSSNEH MSI+PIVGMGG+GKTT A+ +FNH IKGHFD IWVCVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIIIN 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEI 300
ILE IF SLT TS+G K LL+ +QKEM GK YFLVLDDVWNEE+ LW +L DCLK+I
Sbjct: 241 ILEGIFQSLTKTSSGLNHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQI 300
Query: 301 AGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIR 360
AGKSGN IIVTTRS++VA + T+ SH L KLSDDQCW LFKESANAN L MNP+LE IR
Sbjct: 301 AGKSGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKESANANRLSMNPKLEIIR 360
Query: 361 VELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRL 420
LVKKI GVPLVAKVLGGA FEG++ERWVT+V+ VV+NIS DDKDFV SILKLSVD L
Sbjct: 361 DMLVKKIGGVPLVAKVLGGATKFEGDYERWVTKVESVVRNISNDDKDFVSSILKLSVDCL 420
Query: 421 PLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQP--QEGDDEMMIEDIGEEYFNFL 480
PLLSLKQCFAYCSNF KD L+DKE+LI++WIAQGF+QP QEGD+EM EDIGE +FNFL
Sbjct: 421 PLLSLKQCFAYCSNFAKDCLYDKENLIRIWIAQGFIQPQDQEGDNEMTEEDIGEWHFNFL 480
Query: 481 LSRSLFEVVI-----KGLITHFTMHDLIHDIACDISNHQKV--ESSLNNSSEKSVSKLRT 540
LSRSLF+ V +G+ +F MHDLIHDIAC ISNHQK+ E S +N S KS KLRT
Sbjct: 481 LSRSLFQDVTMDKSERGI--YFIMHDLIHDIACAISNHQKLLHELSSSNRSRKSARKLRT 540
Query: 541 LICFKGYKVFNNDLKDHACLRVLVVDRIRCDKNFSCL--IGMFKHLRYLDISYISGYVEL 600
LIC +V N+L D LRVLV + C+K + L IG KHLRYL I+ YV++
Sbjct: 541 LIC--NNQVIYNELVDCVSLRVLV---LHCEKTGNLLESIGKLKHLRYLSIAN-GNYVDV 600
Query: 601 KDVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQF-FCIKTKKMPLHMSQLIHLQTL 660
ESI LYNLQTLKL LPKN RK+V LRHL+F ++MP HMS+LIHLQTL
Sbjct: 601 LP-ESIVMLYNLQTLKLRHMKSLPKNSRKMVSLRHLEFELKSDFRQMPSHMSELIHLQTL 660
Query: 661 SAYEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNS 720
S + A +E G KIEELGPLKNLKG L+L L +V+SK E+I A+L+EKKNL +L+ W
Sbjct: 661 SRFVAGFENGCKIEELGPLKNLKGKLQLSCLERVESKEESIVAKLVEKKNLQELIFEW-- 720
Query: 721 SSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFV 780
SS EG N + S +VLEGLQPH NLQSL++ + GQV ++IFV
Sbjct: 721 ----------SSRFEGKNS-----RYSELEVLEGLQPHKNLQSLTVIGFGGQVLGDAIFV 780
Query: 781 EKLVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNV----TKQWEDVSN--GIAFPHLG 840
E L I L +C+ CE LPMLG+LPNL L+I+SMD++ ++ + + SN G +F L
Sbjct: 781 ENLTVIRLSNCKNCERLPMLGRLPNLVELNINSMDSLRCIGSEFYGNGSNNQGHSFSKLR 840
Query: 841 ILYIENCSKLMS------VPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPN 900
L C L V + F SL TL + GC L +LPN LEGC+S++ L ++ CP
Sbjct: 841 KLTFIWCRNLEQWKDAAVVSNSFGSLQTLSIYGCGNLEKLPNGLEGCSSLRDLTVAYCPK 900
Query: 901 LSVNVSN---------------------WSNLEAVEIIGCMQDYDISPFIH-QSSITHLS 960
LS+NV N +NL+ ++ +GCMQDYD +PF+ QS I L
Sbjct: 901 LSLNVQNCQNLSNLRIDGLKKLPLGLGHLTNLKEMKFVGCMQDYDFTPFVSLQSLIIKLE 960
Query: 961 MTDNSENNASQLPQQLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWF 997
+ D N +QLP LQH+T LK L I F GIE PEWL NL SLE L C+NL
Sbjct: 961 LRDECSGNCTQLPPHLQHLTALKQLSIGNFGGIEVPPEWLNNLTSLEMLSFEGCENLRRL 1012
BLAST of Lag0021704 vs. ExPASy TrEMBL
Match:
A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)
HSP 1 Score: 1001.5 bits (2588), Expect = 2.6e-288
Identity = 575/1061 (54.19%), Postives = 713/1061 (67.20%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAEFLWTFAVQE LKK+V AEQI +AWGLEKELS L WLLKA+ IL DI+ K+ HH
Sbjct: 1 MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
SV +WVE L +YEADDLLDE+VYE +RQ VE KVR SPS NSFLF KM K
Sbjct: 61 SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMAK 120
Query: 121 KLKEVTETLCQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVSN 180
K+K++T+TL + Y EAS G LVG EST ++ L QIRETTS L+FEV GRE EV
Sbjct: 121 KMKKITKTLYEHYCEASPLG---LVGDESTTESEAALNQIRETTSILDFEVEGREAEVLE 180
Query: 181 IVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITN 240
I+KLVIDS++E +S+I IVGMGG+GKTTLAK VFNH IKGHFD+T+WVCVS+PFI+
Sbjct: 181 ILKLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMK 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEI 300
ILE IF LTNTS+G S+E LL L++EMQGK YFLVLDDVW++E LW EL LK I
Sbjct: 241 ILEAIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYI 300
Query: 301 AGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELETIR 360
AGKSGN I+VTTRSV+VAT ++T+ +HL KLSDD CW+L K+SANAN L MN +LE +
Sbjct: 301 AGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENTK 360
Query: 361 VELVKKIDGVPLVAKVLGGAVTF-EGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 420
LV+KI GVPL+AKVLGGAV F EG E W+ +++ +NIS +DKDFVLSILKLSV+
Sbjct: 361 NILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKDFVLSILKLSVES 420
Query: 421 LPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLL 480
LP +LKQCFAYCSNFP+D+ FDK+ IQMWIA+GF+QP++ + + +E+IGEEY NFLL
Sbjct: 421 LPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEYLNFLL 480
Query: 481 SRSLFEVVIK--GLITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVSKLRTLICFKG 540
SRSLFE IK G I F +HDL+HDIAC ISNH K++S+ + + KS KLRTLIC +
Sbjct: 481 SRSLFEDAIKYDGRIVTFKIHDLMHDIACAISNHHKMDSNPISWNGKSTRKLRTLIC-EN 540
Query: 541 YKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDVESIDK 600
+ F+ D CLRVLV+ + S ++ HLRYLDIS + L+D SI
Sbjct: 541 EEAFHKIQTDIICLRVLVLKWFDTN-TLSTIMDKLIHLRYLDISNCNINKLLRD--SICA 600
Query: 601 LYNLQTLKLGE-RIPLPKNLRKLVKLRHLQF-FCIKTKKMPLHMSQLIHLQTLSAYEASY 660
LYNLQTLKLG LPKNLR LV LRHL+F +MP HM +IHLQTLS +
Sbjct: 601 LYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGL 660
Query: 661 EQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNH 720
E+G KI+ELGPLK+LKG+L L NL V++K EA+ A+L+EKK L L+ W N
Sbjct: 661 EKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFL-----NL 720
Query: 721 WNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIF 780
++ E +NK +VLEGLQPH N+QSL I + G+V N+IFVE LV+I
Sbjct: 721 YDRGEYDEDDNK----------QVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIR 780
Query: 781 LKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDV-------SNGIAFPHLGILYIEN 840
L DC +CE+LPMLGQLPNL+ L+I SM++V + N AFP L +I
Sbjct: 781 LVDCGRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICG 840
Query: 841 CSKLMS-------VPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKISDCPNLSVNV 900
KL + F L L L GC +L +LP+ LEGC SI+ L I CPNL +NV
Sbjct: 841 LKKLQQWDEATVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNV 900
Query: 901 SN---------------------WSNLEAVEIIGCMQDYDISPFIHQSS-ITHLSMTD-- 960
N +NL+ + I GCMQ+Y+ SPFIH SS + L +TD
Sbjct: 901 QNLYNLYHLDIRGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDG 960
Query: 961 NSENNASQLPQQLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKNLEWFPSK 1019
+S + +QLPQQLQH+T LKVL+I F+ IE LPEWLGNL L +L CKNL+ PS+
Sbjct: 961 SSGSETTQLPQQLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSR 1020
BLAST of Lag0021704 vs. ExPASy TrEMBL
Match:
A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)
HSP 1 Score: 926.0 bits (2392), Expect = 1.4e-265
Identity = 556/1068 (52.06%), Postives = 698/1068 (65.36%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLH-H 60
MAE + TF VQE LK++VK AEQI VAW LE E+S L + L A+ IL DI+RK+ H
Sbjct: 1 MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
Query: 61 ESVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMT 120
SV+ WVE L+ V+EADDLLDELVYE LR+ VE KV S S NSFLFR KM
Sbjct: 61 NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
Query: 121 KKLKEVTETLCQLYHEASSFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVS 180
KK+K +T+TL Q Y AS+FG LVG E+ + L QIRETTS L+F+V GRE EV
Sbjct: 121 KKIKNITDTLNQHYCAASAFG---LVGVETVTEIELALNQIRETTSILDFQVEGREAEVL 180
Query: 181 NIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIIT 240
++KL IDS+NEH MS+I IVGMGG+GKTTLAK +FNH+ I+GHFD+TIWVCVS+PFI+T
Sbjct: 181 ELLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVT 240
Query: 241 NILEEIFHSLTNTSNGFKS-KENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLK 300
ILE+IF LT T +G +S KE LL L+KEMQ K+YFLVLDDVW+ E LW EL+ CLK
Sbjct: 241 KILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLK 300
Query: 301 EIAGKSGNVIIVTTRSVKVATFMETLSSHHLNKLSDDQCWSLFKESANANGLLMNPELET 360
IAGK GN I+VTTR+ +VAT +E +S + L KLS+DQCW+LFKESANAN L MN +LE
Sbjct: 301 HIAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEI 360
Query: 361 IRVELVKKIDGVPLVAKVLGGAVTF-------EGNFERWVTEVKRVVKNISKDDKDFVLS 420
++ ELV+K+ GVPLVAKVLGGAV F E + W+T+V+ +V+NIS +DKDFVLS
Sbjct: 361 MKKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLS 420
Query: 421 ILKLSVDRLPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEG-DDEMMIEDI 480
ILKLSVD LP LKQC AYCSNF +D+ F K+ LI+MWIAQGF+QP +G D +++EDI
Sbjct: 421 ILKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDI 480
Query: 481 GEEYFNFLLSRSLFEVVIKGL---ITHFTMHDLIHDIACDISNHQKVESSLNNSSEKSVS 540
GE+YFNFLLSRS+F+ V + I F MHDL+HDIAC IS+HQ VES+ NN S KSV
Sbjct: 481 GEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVR 540
Query: 541 KLRTLICFKGYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYV 600
KLRTLIC + N D CLRVL V + + I HLRYLDIS S +
Sbjct: 541 KLRTLICNDEVINYLNQ-NDIVCLRVLKV-IFQSHTDLWIPIDKLIHLRYLDISECS--I 600
Query: 601 ELKDVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQT 660
+ES+ LYNLQTLKLG+ LPKNLRKLV LRHL+F MP M LIHLQ+
Sbjct: 601 NKLLLESLSLLYNLQTLKLGQS-GLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQS 660
Query: 661 LSAYEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWN 720
LS + +E+G KIEELGPLKNLKG L L NL +V++K EA+ A+L+EKKNL L L +
Sbjct: 661 LSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFF 720
Query: 721 SSSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPNSIF 780
+ K G D++G +VLEGLQPH NLQSL I + G+V P IF
Sbjct: 721 ETDKRG------------EDDEDGI----VQVLEGLQPHKNLQSLEILGFRGKVLPTGIF 780
Query: 781 VEKLVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDV-------SNGIAFPH 840
VE LVKI L +CE+LPMLGQLPNL+ L+I M++V + N +AFP
Sbjct: 781 VENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQ 840
Query: 841 LGILYIENCSKL--------MSVPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCLKIS 900
L L I L + + F L + + C L +LP+ LEGC+S++ L I
Sbjct: 841 LKKLSIYEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIR 900
Query: 901 DCPNLSVNVSN---------------------WSNLEAVEIIGCMQDYDISPFIH-QSSI 960
C NL +NV N + L+ ++I GCMQ+Y+ S IH S +
Sbjct: 901 GCFNLMLNVQNLHKLYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQL 960
Query: 961 THLSMTDNSENNASQLPQQLQHITTLKVLRICLFNGIEALPEWLGNLASLESLQLFACKN 1019
L ++ + +QLPQQLQH+T L+VL+I F+ IEALPEW+GNL SL++L+ C
Sbjct: 961 VELELSGRYGSVDTQLPQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFK 1020
BLAST of Lag0021704 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 341.7 bits (875), Expect = 2.2e-93
Identity = 294/1002 (29.34%), Postives = 478/1002 (47.70%), Query Frame = 0
Query: 32 EKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENLQHAVYEADDLLDELVYEDLRQK 91
E L +LS LL A+L D + K++ + V WV L+ VY A+D LD++ E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 92 V----ETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLYHEASSFGVVALVGQ 151
+ + N ++R S D + +L++VT L +L + + G+ L
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155
Query: 152 ESTETNKNILKQIRETTSTLN-FEVIGREVEVSNIVKLVI-DSSNEHPMSIIPIVGMGGV 211
I KQ TTS ++ EV GR+ + I++ +I ++ ++ ++++ IVG+GGV
Sbjct: 156 --------IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 215
Query: 212 GKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFHSLTNTSNGFKSKENLLKG 271
GKTTL++ ++N + ++ +F +W VSE F + I ++++ S+T+ F + L
Sbjct: 216 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 275
Query: 272 LQKEMQGKS--YFLVLDDVWNEEASLWGELKDCLKEIAGKSGNVIIVTTRSVKVATFMET 331
L++ + G + LVLDD+WNE + W L+ I G+ I+VTTRS +VA+ M
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRVASIMCA 335
Query: 332 LSSHHLNKLSDDQCWSLFKESANAN-GLLMNPELETIRVELVKKIDGVPLVAKVLGGAVT 391
+ H+L LSD CWSLF ++ N +N E+ + +V K G+PL K LGG +
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 392 FEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFD 451
FEG W + + ++ DK +L +L++S LP LK+CFAYCS FPK H F+
Sbjct: 396 FEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 455
Query: 452 KESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYFNFLLSRSLFEVVIKGLITHFTMHDLIH 511
K+ ++ +W+A+GF+Q +E++G EYF+ L SRSL ++ T + MHD I+
Sbjct: 456 KDKVVLLWMAEGFLQQTRSSKN--LEELGNEYFSELESRSL----LQKTKTRYIMHDFIN 515
Query: 512 DIA----------------CDISNHQKVESSLNNS--------SEKSVSKLRTLI----- 571
++A +S + S L ++ + + V LRT +
Sbjct: 516 ELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLT 575
Query: 572 -----CFKGYKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVE 631
C V L LRVL + + + H R+LD+S E
Sbjct: 576 NSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLS----RTE 635
Query: 632 LKDV-ESIDKLYNLQTLKL---GERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIH 691
L+ + +S+ +YNLQTL L LP ++ L+ LR+L K ++MP +L
Sbjct: 636 LEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKS 695
Query: 692 LQTLSAYEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVL 751
LQTL+ + S G +I ELG L +L G LK++ L +V A+A A L KK+L ++
Sbjct: 696 LQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDF 755
Query: 752 TWNSSSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFPN 811
W + S NN++ N+ + V E L+PH +++ L+I Y G+ FP+
Sbjct: 756 VWRTGSSSSE--NNTNPHRTQNEAE---------VFEKLRPHRHIEKLAIERYKGRRFPD 815
Query: 812 SI---FVEKLVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHL 871
+ ++V I L++C+ C LP LGQLP L+ L IS M + S G F
Sbjct: 816 WLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQ------SIGRKF--- 875
Query: 872 GILYIENCSKLMSVPHCFVSLHTLFLEGCKELRE----LPNQLEGCNSIKCLKISDCPNL 931
Y + F SL TL + + +E + + S+K L I CP L
Sbjct: 876 ---YFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPEL 935
Query: 932 SVNVSNW-SNLEAVEIIGCMQDYDISPFIHQSSITHLSMTDNSENNASQLPQQLQHITTL 976
+ + + +L ++ I C D P H+ S +L + + + L H L
Sbjct: 936 TGTLPTFLPSLISLHIYKCGL-LDFQPDHHEYSYRNLQTLSIKSSCDTLVKFPLNHFANL 991
BLAST of Lag0021704 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 285.8 bits (730), Expect = 1.4e-76
Identity = 261/911 (28.65%), Postives = 426/911 (46.76%), Query Frame = 0
Query: 35 LSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENLQHAVYEADDLLDELVYEDLRQKVET 94
L +L L+ A +L D D++ H V+ W+ ++ A ++A+D+LDEL E LR++V
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 95 QNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLY-HEASSFGVVALVGQESTETN 154
+ N R + KK++ E + +L H V+ L +E +ET
Sbjct: 96 E-----AGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGL--KEYSETR 155
Query: 155 KNILKQIRET--TSTLNFEVIGREVEVSNIVKLVI--DSSNEHPMSIIPIVGMGGVGKTT 214
+ +Q + ++GR + +V L++ D + ++I +VGM GVGKTT
Sbjct: 156 EPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTT 215
Query: 215 LAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIFHSLTNTSNGFKSKENLLKGLQKE 274
L + VFN + HF+ +W+ F + + + + +T+++ + +L L+K
Sbjct: 216 LTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKT 275
Query: 275 MQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGNVIIVTTRSVKVATFMETLSSHHL 334
+ GK + LVLDD W+E S W + + + G+ I++TTRS V+T + + +
Sbjct: 276 LSGKRFLLVLDDFWSESDSEWESFQVAFTD--AEEGSKIVLTTRSEIVSTVAKAEKIYQM 335
Query: 335 NKLSDDQCWSLFKESANANGLL--MNPELETIRVELVKKIDGVPLVAKVLGGAVTFEGNF 394
+++++CW L A N + +N ELE I + ++ G+PL A+ + + + N
Sbjct: 336 KLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNP 395
Query: 395 ERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFDKESLI 454
+ W V KN S +L +LKLS D LP LK+CFA CS FPK H+FD+E L+
Sbjct: 396 DDWYA----VSKNFSSYTNS-ILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFDREELV 455
Query: 455 QMWIAQGFV-QPQEGDDEMMIEDIGEEYFNFLLSRSLFEVVIKGLITHFTMHDLIHDIAC 514
+W+A + QP+ +EDIG +Y L+++S F+ + +T F MHDL++D+A
Sbjct: 456 LLWMAIDLLYQPR---SSRRLEDIGNDYLGDLVAQSFFQ-RLDITMTSFVMHDLMNDLAK 515
Query: 515 DIS------------------------NHQKVESSLNNSSEKSVSKLRTLICFKG----- 574
+S + + ++S+ S LRT++ F
Sbjct: 516 AVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLE 575
Query: 575 -----YKVFNNDLKDHACLRVLVVDRIRCDKNFSCLIGMFKHLRYLDISYISGYVELKDV 634
KV N L + LR+L + + L G+ K LRYLD+S ++K++
Sbjct: 576 SLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGL-KLLRYLDLS----STKIKEL 635
Query: 635 -ESIDKLYNLQTLKLG---ERIPLPKNLRKLVKLRHLQFFCIKTKKMPLHMSQLIHLQTL 694
E + L NLQTL L + LPK++ +L+ LR L +MP + +L LQ L
Sbjct: 636 PEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKL 695
Query: 695 SAYEASYEQGRKIEELGPLKNLKGSLKLLNLGKVKSKAEAIGARLIEKKNLIDLVLTWNS 754
S + G + EL L +L+G+L++ L V +EA A L K L L+L W
Sbjct: 696 SNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKW-- 755
Query: 755 SSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEGLQPHHNLQSLSINLYSGQVFP----- 814
+ +G+ + S N C +VL L+PH +L++ I Y G FP
Sbjct: 756 -TVKGSGFVPGSF--------NALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGD 815
Query: 815 NSIFVEKLVKIFLKDCRKCEMLPMLGQLPNLEALDISSMDNVTKQ------WEDVSNGIA 874
+S F + + L C C LP +GQLP+L+ L I + + K E+ S G+
Sbjct: 816 SSFF--GITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVP 875
Query: 875 FPHLGILYIEN--------CSKLMSVPHCFVSLHTLFLEGCKELRELPNQLEGCNSIKCL 881
F L IL C +L F L L ++ C LR+ EG S +
Sbjct: 876 FQSLQILKFYGMPRWDEWICPELED--GIFPCLQKLIIQRCPSLRK--KFPEGLPSSTEV 905
BLAST of Lag0021704 vs. TAIR 10
Match:
AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 216.9 bits (551), Expect = 8.0e-56
Identity = 235/881 (26.67%), Postives = 396/881 (44.95%), Query Frame = 0
Query: 10 VQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENL 69
V FL+K + + E+ K+L L + L ++ L+D +R++ +E++R V +L
Sbjct: 6 VTVFLEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
+ VYEA+D+L + D E +++ + + +YK +K+L+E+ E +
Sbjct: 66 RELVYEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPA---RVPLQYKKSKRLQEINERI 125
Query: 130 CQLYHEAS---SFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVSNIVKLVI 189
++ + F + VG+++ + + + T + E R+ +K +
Sbjct: 126 TKIKSQVEPYFEFITPSNVGRDNGTDRWS--SPVYDHTQVVGLEGDKRK------IKEWL 185
Query: 190 DSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIF 249
SN+ + I+ VGMGG+GKTT+A+ VFN K I+ F+ IWV VS+ F I+ I
Sbjct: 186 FRSNDSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSIL 245
Query: 250 HSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGN 309
+L + S G LL+ +Q+ + GK Y +V+DDVW++ S W ++ L G+ G+
Sbjct: 246 RNLGDASVG-DDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR--GQGGS 305
Query: 310 VIIVTTRSVKVATFMETL--SSHHLNKLSDDQCWSLFKESANA--NGLLMNPELETIRVE 369
V IVTTRS VA ++ +H LS D W LF A A +G PELE + E
Sbjct: 306 V-IVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKE 365
Query: 370 LVKKIDGVPLVAKVLGGAVTFEGN-FERWVTEVKRVVKNISKD------DKDFVLSILKL 429
+V K G+PL K +GG + + + + W +R+ ++ + + D V+S L+L
Sbjct: 366 IVTKCKGLPLTIKAVGGLLLCKDHVYHEW----RRIAEHFQDELRGNTSETDNVMSSLQL 425
Query: 430 SVDRLPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYF 489
S D LP LK C S +P+D + K+ L+ WI +GFV + G + GE+ F
Sbjct: 426 SYDELP-SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNG---RSATESGEDCF 485
Query: 490 NFLLSRSLFEVVIK---GLITHFTMHDLIHDIACDIS-------------NHQKVESSLN 549
+ L +R L EVV K G I +HD++ D+ DI+ H + + +
Sbjct: 486 SGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFD 545
Query: 550 NSSEKSVSKLRTLICFK---GYKVFNNDL----KDHACLRVLVVDRIRCDKNFSCL---I 609
K KLR ++ N+DL D LRVL + + D S + I
Sbjct: 546 EKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEI 605
Query: 610 GMFKHLRYLDISYISGYVELKDVESIDKLYNLQTL------KLGERIPLPKNLRKLVKLR 669
+HL L +S ++ S++ L+NLQ L L + P +KL+ L
Sbjct: 606 ASLQHLACLSLSNTHPLIQFP--RSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLD 665
Query: 670 HLQFFCIKTKKMPLHMSQLIHLQTLSAYE-ASYEQGRKIEELGPLKNLKGSLKLLNLGKV 729
C + P + L+ L+ L ++ A G K+ E+ L NL+ LG
Sbjct: 666 MTN--CGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLR------KLGLS 725
Query: 730 KSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEG 789
++ + IE++ L L+ S N +++ DD ++
Sbjct: 726 LTRGDQ-----IEEEELDSLINLSKLMSISINCYDSYG-------DD------LITKIDA 785
Query: 790 LQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIFLKDCRKCEMLPMLGQLP---------N 835
L P H L LS+ Y G+ P+ + KL + ++ M Q P
Sbjct: 786 LTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMSICSGNLVKM--QEPFWGNENTHWR 831
BLAST of Lag0021704 vs. TAIR 10
Match:
AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 216.9 bits (551), Expect = 8.0e-56
Identity = 235/881 (26.67%), Postives = 396/881 (44.95%), Query Frame = 0
Query: 10 VQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHESVRMWVENL 69
V FL+K + + E+ K+L L + L ++ L+D +R++ +E++R V +L
Sbjct: 6 VTVFLEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
+ VYEA+D+L + D E +++ + + +YK +K+L+E+ E +
Sbjct: 66 RELVYEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPA---RVPLQYKKSKRLQEINERI 125
Query: 130 CQLYHEAS---SFGVVALVGQESTETNKNILKQIRETTSTLNFEVIGREVEVSNIVKLVI 189
++ + F + VG+++ + + + T + E R+ +K +
Sbjct: 126 TKIKSQVEPYFEFITPSNVGRDNGTDRWS--SPVYDHTQVVGLEGDKRK------IKEWL 185
Query: 190 DSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFIITNILEEIF 249
SN+ + I+ VGMGG+GKTT+A+ VFN K I+ F+ IWV VS+ F I+ I
Sbjct: 186 FRSNDSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSIL 245
Query: 250 HSLTNTSNGFKSKENLLKGLQKEMQGKSYFLVLDDVWNEEASLWGELKDCLKEIAGKSGN 309
+L + S G LL+ +Q+ + GK Y +V+DDVW++ S W ++ L G+ G+
Sbjct: 246 RNLGDASVG-DDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR--GQGGS 305
Query: 310 VIIVTTRSVKVATFMETL--SSHHLNKLSDDQCWSLFKESANA--NGLLMNPELETIRVE 369
V IVTTRS VA ++ +H LS D W LF A A +G PELE + E
Sbjct: 306 V-IVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKE 365
Query: 370 LVKKIDGVPLVAKVLGGAVTFEGN-FERWVTEVKRVVKNISKD------DKDFVLSILKL 429
+V K G+PL K +GG + + + + W +R+ ++ + + D V+S L+L
Sbjct: 366 IVTKCKGLPLTIKAVGGLLLCKDHVYHEW----RRIAEHFQDELRGNTSETDNVMSSLQL 425
Query: 430 SVDRLPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDIGEEYF 489
S D LP LK C S +P+D + K+ L+ WI +GFV + G + GE+ F
Sbjct: 426 SYDELP-SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNG---RSATESGEDCF 485
Query: 490 NFLLSRSLFEVVIK---GLITHFTMHDLIHDIACDIS-------------NHQKVESSLN 549
+ L +R L EVV K G I +HD++ D+ DI+ H + + +
Sbjct: 486 SGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFD 545
Query: 550 NSSEKSVSKLRTLICFK---GYKVFNNDL----KDHACLRVLVVDRIRCDKNFSCL---I 609
K KLR ++ N+DL D LRVL + + D S + I
Sbjct: 546 EKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEI 605
Query: 610 GMFKHLRYLDISYISGYVELKDVESIDKLYNLQTL------KLGERIPLPKNLRKLVKLR 669
+HL L +S ++ S++ L+NLQ L L + P +KL+ L
Sbjct: 606 ASLQHLACLSLSNTHPLIQFP--RSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLD 665
Query: 670 HLQFFCIKTKKMPLHMSQLIHLQTLSAYE-ASYEQGRKIEELGPLKNLKGSLKLLNLGKV 729
C + P + L+ L+ L ++ A G K+ E+ L NL+ LG
Sbjct: 666 MTN--CGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLR------KLGLS 725
Query: 730 KSKAEAIGARLIEKKNLIDLVLTWNSSSKEGNHWNNSSSGEGNNKDDNGFQCSSFKVLEG 789
++ + IE++ L L+ S N +++ DD ++
Sbjct: 726 LTRGDQ-----IEEEELDSLINLSKLMSISINCYDSYG-------DD------LITKIDA 785
Query: 790 LQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIFLKDCRKCEMLPMLGQLP---------N 835
L P H L LS+ Y G+ P+ + KL + ++ M Q P
Sbjct: 786 LTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMSICSGNLVKM--QEPFWGNENTHWR 831
BLAST of Lag0021704 vs. TAIR 10
Match:
AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 204.9 bits (520), Expect = 3.2e-52
Identity = 235/940 (25.00%), Postives = 430/940 (45.74%), Query Frame = 0
Query: 1 MAEFLWTFAVQEFLKKLVKLAAEQIGVAWGLEKELSKLSNWLLKAEAILRDIDRKRLHHE 60
MAE + +F V+ KL +L + + G+++++ L L + +++L+D D K+ E
Sbjct: 1 MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60
Query: 61 SVRMWVENLQHAVYEADDLLDELVYEDLRQKVETQNTKEKVRKFFSPSDNSFLFRYKMTK 120
VR ++E+++ VY+ADD+++ + +LR K + K++VR + R K
Sbjct: 61 RVRNFLEDVKDIVYDADDIIESFLLNELRGK--EKGIKKQVRTLAC----FLVDRRKFAS 120
Query: 121 KLKEVTETLCQLYHEASSFGV--VALVGQESTETNKNILKQIRETTS-TLNFEVIGREVE 180
++ +T+ + ++ S G+ +A G S + ++IR+T S +++G +
Sbjct: 121 DIEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQER-QREIRQTFSRNSESDLVGLDQS 180
Query: 181 VSNIVKLVIDSSNEHPMSIIPIVGMGGVGKTTLAKTVFNHKLIKGHFDETIWVCVSEPFI 240
V +V ++++ + + ++ + GMGG+GKTTLA+ VF+H +++ HFD WVCVS+ F
Sbjct: 181 VEELVDHLVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFT 240
Query: 241 ITNILEEIFHSLTNTSNG-FKSKENLLKG-LQKEMQGKSYFLVLDDVWNEEASLWGELKD 300
++ + I L G + E L+G L + ++ Y LVLDDVW EE W +K
Sbjct: 241 RKDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKA 300
Query: 301 CLKEIAGKSGNVIIVTTRSVKVATFME-TLSSHHLNKLSDDQCWSLFKESANANGLLMNP 360
K G +++T+R+ + + T + L+ +Q W LF+ ++
Sbjct: 301 VFPH---KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKTEF 360
Query: 361 EL-ETIRVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNI------SKDDKD 420
++ E + E+V G+PL KVLGG + + W +V +I S D+ +
Sbjct: 361 KVDEAMGKEMVTYCGGLPLAVKVLGGLLAKKHTVLEWKRVHSNIVTHIVGKSGLSDDNSN 420
Query: 421 FVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMI 480
V +L LS + LP + LK CF Y ++FP+D+ D + L W+A+G + P D I
Sbjct: 421 SVYRVLSLSYEDLP-MQLKHCFFYLAHFPEDYKIDVKILFNYWVAEGIITPFH--DGSTI 480
Query: 481 EDIGEEYFNFLLSRSLF---EVVIKGLITHFTMHDLIHDIACDISNHQ------KVESSL 540
+D GE Y L+ R++ E + I + MHD++ ++ + + KV ++
Sbjct: 481 QDTGESYLEELVRRNMVVVEESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVVKVPTTT 540
Query: 541 NNS-SEKSVSKLRTLICFKGYKVFNNDLKDHACLRVLVV----DRIRCDKNFSCLIGMFK 600
+ + + +S + R L+ G + KD+ R +++ ++ + F CL
Sbjct: 541 STTINAQSPCRSRRLVLHSGNALHMLGHKDNKKARSVLIFGVEEKFWKPRGFQCL----P 600
Query: 601 HLRYLDISYISGYVELKDVESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFFCIKTK 660
LR LD+SY+ E LP ++ L+ LR L +
Sbjct: 601 LLRVLDLSYVQ----------------------FEGGKLPSSIGDLIHLRFLSLYEAGVS 660
Query: 661 KMPLHMSQLIHLQTLSAYEASYEQGRKIEELGPLKNLKGSLKLLNLGK-VKSKAEAIGAR 720
+P S L +L+ L + LK ++ L+ L L + + +K +
Sbjct: 661 HLP---SSLGNLKLLLCLNLGVADRLLVHVPNVLKEMQ-ELRYLRLPRSMPAKTK----- 720
Query: 721 LIEKKNLIDLVLTWNSSSKEG-----------NHWNNSSSGEGNNKDDNGFQCSSFKVLE 780
+E +L++L N S+K G + N SGE + S + L
Sbjct: 721 -LELGDLVNLESLTNFSTKHGSVTDLLRMTKLSVLNVIFSGECTFET----LLLSLRELR 780
Query: 781 GLQ--PHHNLQSLSINLYSGQV-------------------FPNSI-FVEKLVKIFLKDC 840
L+ H+ Q +S+ + G++ FP+ F L I+L C
Sbjct: 781 NLETLSFHDFQKVSVANHGGELLVLDFIHLKDLTLSMHLPRFPDQYRFPPHLAHIWLIGC 840
Query: 841 RKCE-MLPMLGQLPNLEALDISSMDNVTKQWEDVSNGIAFPHLGILYIENCSKLM----- 872
R E +P+L +L +L+++ +SS + ++ G FP L L + +L+
Sbjct: 841 RMEEDPMPILEKLLHLKSVYLSSGAFLGRRMVCSKGG--FPQLLALKMSYKKELVEWRVE 872
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038877313.1 | 0.0e+00 | 64.23 | putative disease resistance protein RGA3 [Benincasa hispida] | [more] |
XP_022145229.1 | 0.0e+00 | 63.08 | putative disease resistance protein RGA4 [Momordica charantia] | [more] |
XP_038895409.1 | 0.0e+00 | 62.68 | disease resistance protein RGA2-like isoform X1 [Benincasa hispida] | [more] |
XP_022145177.1 | 0.0e+00 | 60.37 | putative disease resistance protein RGA3 [Momordica charantia] | [more] |
XP_038895415.1 | 3.4e-306 | 60.06 | disease resistance protein RGA2-like isoform X2 [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
Q7XA40 | 9.4e-118 | 32.23 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XBQ9 | 6.7e-116 | 32.88 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
Q7XA42 | 3.5e-112 | 31.37 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XA39 | 3.8e-111 | 31.40 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q9LRR4 | 3.0e-92 | 29.34 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CUL8 | 0.0e+00 | 63.08 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A6J1CVR6 | 0.0e+00 | 60.37 | putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A6J1CU86 | 5.2e-305 | 58.54 | disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014... | [more] |
A0A0A0KST5 | 2.6e-288 | 54.19 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1 | [more] |
A0A0A0LBV0 | 1.4e-265 | 52.06 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1 | [more] |