Lag0021683 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0021683 vs. NCBI nr
Match: XP_008453950.1 (PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >TYK16967.1 salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 703.0 bits (1813), Expect = 1.7e-198
Identity = 356/476 (74.79%), Postives = 405/476 (85.08%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVKVLS+EN++PSSPTPPHLKT +LSLLDQFSPVLY+PL++FY  +NKDD   HQ+H++
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 120
           LMATLK+SLSKTL+HFYLLAGRIVD +IRCSDEGAVF+EA+VSCSMSEILK+PNNE LMK
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCSDEGAVFIEATVSCSMSEILKQPNNEFLMK 120

Query: 121 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASI---- 180
           L+P S R T P+EE  H++VQ NVFDCGGIAISLC LHKLMDATTI CFLK WA+I    
Sbjct: 121 LIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRRS 180

Query: 181 --AANLAYD------------------DVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEA 240
               NL  D                  D FS+M CYDYKELSSLFPQTN LPFHPRL EA
Sbjct: 181 FTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHEA 240

Query: 241 LSSGSEEESCFQRFVFKEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG- 300
           LSS  EE+S FQRFVFKEKAILDLKA+AKS DVPNPTSVEVLSGFIWKCALEAAST  G 
Sbjct: 241 LSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLGL 300

Query: 301 SQMPSVLTHAVNLRKRMEPPLPEFSVGNLFWNVVAHYTAD-EAQVELSELVGLIRQSFGE 360
           SQ+PS+LTHAVNLRKRMEPPLPEFSVGN+FWNVVAHY AD + QVELSELV LIRQSF +
Sbjct: 301 SQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADKKTQVELSELVSLIRQSFVD 360

Query: 361 INGNNIRKLVGDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIG 420
           INGN I+K+VG+EGSEA+VKLV ERN  LF IPKLYICTS R+MDL+EVNFGWGKP+WIG
Sbjct: 361 INGNYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFGWGKPIWIG 420

Query: 421 SAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           SAG+S+TTIKNMIVLLD++S+DGVE W+ILE+ EM++LL+NQEFL FAL+NPAIP+
Sbjct: 421 SAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFALLNPAIPI 476

BLAST of Lag0021683 vs. NCBI nr
Match: XP_004152282.1 (BAHD acyltransferase BIA1 [Cucumis sativus])

HSP 1 Score: 700.3 bits (1806), Expect = 1.1e-197
Identity = 357/477 (74.84%), Postives = 404/477 (84.70%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVKVLS+ENI+PSSPTPPHLKT +LSLLDQFSPVLY+PL++FY+ +NKDD   HQ+H+K
Sbjct: 1   MEVKVLSKENIMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYTMDNKDDPRHHQSHEK 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 120
           LMATLK+SLSKTL+HFYLLAGRIVD +I CSDEGAVF+EA+VSCSMSEILK+PNNE LMK
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSICCSDEGAVFIEATVSCSMSEILKQPNNEFLMK 120

Query: 121 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASI---- 180
           L+P S R T P+EE  H++VQVNVFDCGGIAISLC LHKLMDATTI CFLK WA+I    
Sbjct: 121 LVPCSERCTKPIEEYAHVIVQVNVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINKRS 180

Query: 181 --AANLAYD-------------------DVFSEMSCYDYKELSSLFPQTNLLPFHPRLLE 240
               NL  D                   D FS+M CYDYKELSSLFPQTN LPFHPRL E
Sbjct: 181 FATTNLTIDNGASELFTPPSSMTNDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 ALSSGSEEESCFQRFVFKEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG 300
           ALSS  EE+S FQRFVFKEKAILDLKA+AKS DVPNPTSVEVLSGFIWKCALEAAST  G
Sbjct: 241 ALSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLG 300

Query: 301 -SQMPSVLTHAVNLRKRMEPPLPEFSVGNLFWNVVAHYTAD-EAQVELSELVGLIRQSFG 360
            SQ+PS+LTHAVNLRKRMEPPLPEFSVGN+FWNVVAHY AD + QVELSELV LIRQSF 
Sbjct: 301 LSQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADKKTQVELSELVSLIRQSFV 360

Query: 361 EINGNNIRKLVGDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWI 420
           +INGN I+++VG+EGSEA+VKLV ERN  LF IPKLYICTS R+MDL+EVNFGWGKPVWI
Sbjct: 361 DINGNYIKRIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFGWGKPVWI 420

Query: 421 GSAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           GSAG+S+TTIKNMIVLLD++S+DGVE W+ILE+ EM++LLQNQEF  FAL+NP IP+
Sbjct: 421 GSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLQNQEFFNFALLNPPIPI 477

BLAST of Lag0021683 vs. NCBI nr
Match: KGN53114.2 (hypothetical protein Csa_014413 [Cucumis sativus])

HSP 1 Score: 662.1 bits (1707), Expect = 3.3e-186
Identity = 339/457 (74.18%), Postives = 383/457 (83.81%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVKVLS+ENI+PSSPTPPHLKT +LSLLDQFSPVLY+PL++FY+ +NKDD   HQ+H+K
Sbjct: 1   MEVKVLSKENIMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYTMDNKDDPRHHQSHEK 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 120
           LMATLK+SLSKTL+HFYLLAGRIVD +I CSDEGAVF+EA+VSCSMSEILK+PNNE LMK
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSICCSDEGAVFIEATVSCSMSEILKQPNNEFLMK 120

Query: 121 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASI---- 180
           L+P S R T P+EE  H++VQVNVFDCGGIAISLC LHKLMDATTI CFLK WA+I    
Sbjct: 121 LVPCSERCTKPIEEYAHVIVQVNVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINKRS 180

Query: 181 --AANLAYD-------------------DVFSEMSCYDYKELSSLFPQTNLLPFHPRLLE 240
               NL  D                   D FS+M CYDYKELSSLFPQTN LPFHPRL E
Sbjct: 181 FATTNLTIDNGASELFTPPSSMTNDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 ALSSGSEEESCFQRFVFKEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG 300
           ALSS  EE+S FQRFVFKEKAILDLKA+AKS DVPNPTSVEVLSGFIWKCALEAAST  G
Sbjct: 241 ALSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLG 300

Query: 301 -SQMPSVLTHAVNLRKRMEPPLPEFSVGNLFWNVVAHYTAD-EAQVELSELVGLIRQSFG 360
            SQ+PS+LTHAVNLRKRMEPPLPEFSVGN+FWNVVAHY AD + QVELSELV LIRQSF 
Sbjct: 301 LSQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADKKTQVELSELVSLIRQSFV 360

Query: 361 EINGNNIRKLVGDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWI 420
           +INGN I+++VG+EGSEA+VKLV ERN  LF IPKLYICTS R+MDL+EVNFGWGKPVWI
Sbjct: 361 DINGNYIKRIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFGWGKPVWI 420

Query: 421 GSAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEME 431
           GSAG+S+TTIKNMIVLLD++S+DGVE W  L   +++
Sbjct: 421 GSAGNSNTTIKNMIVLLDAISNDGVEAWDSLRNRKLK 457

BLAST of Lag0021683 vs. NCBI nr
Match: XP_022138192.1 (BAHD acyltransferase BIA1-like [Momordica charantia])

HSP 1 Score: 534.3 bits (1375), Expect = 1.0e-147
Identity = 273/460 (59.35%), Postives = 345/460 (75.00%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVK+LSRE I+PSSPTPPHLKT +LSL+DQ SP LY PLL FY   NK D  TH N  K
Sbjct: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCS-DEGAVFVEASVSCSMSEILKEPNNELLM 120
            +ATLK SLSKTLT+F+LLAGRI +T+IRCS DEGAVF+EA+V+C++S+ILKEPNNE+L 
Sbjct: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120

Query: 121 KLLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAAN 180
           KLLP S++ T P EE   IV Q N+F+CGGI ISLC LHKLMD  + SCFLKSWASI   
Sbjct: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180

Query: 181 LAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEA---LSSGSEEESCFQRFVFKEKA 240
            + D+    + C +YK  SSLFPQTNLL FH ++ E    + S S+   C QRFVF  KA
Sbjct: 181 -SLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKA 240

Query: 241 ILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG----SQMPSVLTHAVNLRKRM 300
           I DLKA+AKS +VPNPTSVE L+GFIW CA++  +T  G    SQ PS L+H VNLRKRM
Sbjct: 241 ISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRM 300

Query: 301 EPPLPEFSVGNLFWNVVAHYTAD--EAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSE 360
           EPPLPE S+GN+ W +VAHY +   E QVEL +LV L+RQSF +IN + I+ L+G++G+ 
Sbjct: 301 EPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNR 360

Query: 361 AIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLL 420
           A+++L+GE NK    +P  Y  TS R+MDLNEV+FGWGKP+WIG AG+S+   K+ I+LL
Sbjct: 361 AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLL 420

Query: 421 DSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           D+ S DG+E WM+L++ +M+LL QN +FLEFA +NP+I +
Sbjct: 421 DTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 458

BLAST of Lag0021683 vs. NCBI nr
Match: KAG6582063.1 (Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 501.5 bits (1290), Expect = 7.5e-138
Identity = 270/453 (59.60%), Postives = 331/453 (73.07%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYS-TNNKDDNPTHQNHD 60
           MEVKVLSRE IIPSSPTPPHLK+ KLSLLDQ SP+LY+PLL FY  T   D NPT     
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKSFKLSLLDQLSPMLYTPLLFFYPITKTYDPNPT----- 60

Query: 61  KLMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLM 120
             MATLK SLS+TLT FYLLAGRIV  +I CSD+GAVF+EASVSC+MS ILK+PNNE+L 
Sbjct: 61  --MATLKDSLSQTLTRFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVLR 120

Query: 121 KLLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAAN 180
           KLLP S+  ++P EE P +VVQ N+F CGGIAISLC LHKLMD  T + FL SWAS   N
Sbjct: 121 KLLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWAS--TN 180

Query: 181 LAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKEKAILD 240
               D  S M CYDYK  SSLFPQTNLLPFH    + L+  +  + C +RFVF  K++L+
Sbjct: 181 KGSLDSSSNMICYDYKAFSSLFPQTNLLPFH----QKLAKPANRKRCLRRFVFGTKSLLN 240

Query: 241 LKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRMEPPLPEF 300
           LKA+A+S+ VPNP+ VE L+GFIWK A+ AA      Q  S L+H VNLR RM+P LPE 
Sbjct: 241 LKAKAESRAVPNPSCVEALTGFIWKHAMMAAD----CQGSSTLSHVVNLRGRMKPALPEV 300

Query: 301 SVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNI-RKLVG-DEGSEAIVKLVG 360
           SVGN+ W  VAH +A E ++EL  LVGL+RQSF EIN + I R L+G D+G EA++K VG
Sbjct: 301 SVGNIMWGAVAHSSAME-RLELRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVG 360

Query: 361 ERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDSVSSDG 420
           E NK  +G+   Y CTS R+M LNEV+FGWG+P WI  AG ++  + N+IVL+D V  DG
Sbjct: 361 EMNK--WGMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAGDTNELMANVIVLVDGVMGDG 420

Query: 421 VEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           +E W++L++ EME+L QN +FLEFAL+NP+I L
Sbjct: 421 IEAWILLDEKEMEILEQNPQFLEFALLNPSIHL 433

BLAST of Lag0021683 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.7e-53
Identity = 159/465 (34.19%), Postives = 237/465 (50.97%), Query Frame = 0

Query: 3   VKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDKLM 62
           V+V+S+E I P +PTP  L+   +SLLDQ+S ++Y P++LFY   + D N T   H   +
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAAS-DANSTGSKHHDDL 68

Query: 63  ATLKTSLSKTLTHFYLLAGRIVDT-TIRCSDEGAVFVEASVSCSMSEILKEPNNELLMKL 122
             LK SLS+TL HFY +AGR+ D  T+ C+DEG  F E  +   M + + + +  L + L
Sbjct: 69  HLLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDAHLSL-L 128

Query: 123 LPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAANLA 182
           LP  V  T+ V+E   ++VQVN+FDCGG AI  C  +K+ DA T+  F++S A    N+A
Sbjct: 129 LPSEVASTNFVKEA-QVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAG-TTNIA 188

Query: 183 YDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALS-----SGSEEESCFQRFVFKEKA 242
                S  +    + L   F  T+L P   +L   +S     S S ++   +RFVF    
Sbjct: 189 RRG-SSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAK 248

Query: 243 ILDLKARAKS-----KDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKR 302
           I   + + +S          PT VEV+S  IWK A+++A   S     S +THA+N RK+
Sbjct: 249 ITSAREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVKSAPPGS----ISTVTHAMNFRKK 308

Query: 303 MEPPLPEFSVGNLFWNVVA-----------------HYTADEAQV---ELSELVGLIRQS 362
           M+PPL + S GNL   V A                   T++E QV   ELS+ V L+R+ 
Sbjct: 309 MDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDFVALLRRE 368

Query: 363 FGEINGNN------IRKLV-GDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVN 422
             ++ G+       I+K + G + S A    V ++   LF        TS       E +
Sbjct: 369 IDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALF-------MTSWCKFGFYEAD 428

Query: 423 FGWGKPVWIGSAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEM 430
           FGWG PVW+ +        KNM+ + D    +G+EVW+   + +M
Sbjct: 429 FGWGTPVWVTTVPLIEPKYKNMVFMNDMKCGEGIEVWVNFLEDDM 457

BLAST of Lag0021683 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.9e-52
Identity = 168/488 (34.43%), Postives = 245/488 (50.20%), Query Frame = 0

Query: 3   VKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPV-LYSPLLLFYSTNNKDDNPTHQNHDKL 62
           V+V+S+E I P++PTP  LK   LSLLDQ  P+  Y P++LFY     +   +  +HD L
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 63  MATLKTSLSKTLTHFYLLAGRIVDT-TIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 122
              LK+SLSKTL HFY +AGR++D   + C D+G  F +  +   M E + +P+   L +
Sbjct: 69  -DLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPDVP-LSQ 128

Query: 123 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAANL 182
           LLP  V      +E   ++VQVN+FDCGG AI     HK+ DA T+S F++SWAS     
Sbjct: 129 LLPSEVVSASVPKEA-LVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTS 188

Query: 183 AYDDVFSEMSCYDYK-----ELSSLFPQTNLLPFHPRLLEALSSGSEEE-----SCFQRF 242
                 S  +  D K     + +SLFP +  L     + E   S + E+     +  +RF
Sbjct: 189 RSGG--STAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKRF 248

Query: 243 VF--------KEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVL 302
           VF        +EK  + +    KS+     T VEV++  IWK  ++  ST +G     V+
Sbjct: 249 VFDFAKITSVREKLQVLMHDNYKSR---RQTRVEVVTSLIWKSVMK--STPAG--FLPVV 308

Query: 303 THAVNLRKRMEPPLPEFSVGNLFWNVVA-----------------HYTADEAQVELSELV 362
            HAVNLRK+M+PPL + S GNL   V A                 + T+ E+QV L EL 
Sbjct: 309 HHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHELH 368

Query: 363 GLIRQSFGEINGNNIRKLVGDEGS------------EAIVKLVGERNKLLFGIPKLYICT 422
             I Q   EI+     K+ GD+GS            +A +K + +   + F     +I +
Sbjct: 369 DFIAQMRSEID-----KVKGDKGSLEKVIQNFASGHDASIKKINDVEVINF-----WISS 428

Query: 423 SCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDSVSSDGVEVW-MILEKGEMELL 441
            CR M L E++FGWGKP+W+ +   +    KN   + D+   +G+EVW   LE    +  
Sbjct: 429 WCR-MGLYEIDFGWGKPIWV-TVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFE 472

BLAST of Lag0021683 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.2e-49
Identity = 136/440 (30.91%), Postives = 229/440 (52.05%), Query Frame = 0

Query: 2   EVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDKL 61
           ++++LS E I PSSPTP  LKT KLS LDQ     + P++LFY  N  D N       + 
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFY-PNQLDSNLDRAQRSE- 63

Query: 62  MATLKTSLSKTLTHFYLLAGRI-VDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 121
              LK SLS  LT FY LAGRI +++++ C+D G  F+EA V   +SE +K    + L +
Sbjct: 64  --NLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQ 123

Query: 122 LL---PYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIA 181
            L   PY       +++   + V+++ F+CGG AI +C  HK+ DA +++ FL SW +  
Sbjct: 124 YLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTA-- 183

Query: 182 ANLAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGS---EEESCFQRFVFKE 241
                       +C +  E   + P  +L   H   +E++ +     +E    +RFVF +
Sbjct: 184 ------------TCQE--ETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDK 243

Query: 242 KAILDLKARAKSK--DVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRM 301
           + +  LKA+  S   +V N + V+++   IWK  ++       ++   V   AVNLR RM
Sbjct: 244 EKLEALKAQLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRM 303

Query: 302 EPPLPEFSVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSEAI 361
            PP P+ ++GN+       Y   E   + S+LVG ++ S  +I+  ++++L      + +
Sbjct: 304 NPPFPQSAMGNI---ATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKEL-----QKGV 363

Query: 362 VKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDS 421
             L  E         +L+  +S   +   +++FGWGKPV   S  +++  +KN++ L+D+
Sbjct: 364 TYLDYEAEP-----QELFSFSSWCRLGFYDLDFGWGKPV---SVCTTTVPMKNLVYLMDT 407

Query: 422 VSSDGVEVWMILEKGEMELL 433
            + DG+E W+ + + EM +L
Sbjct: 424 RNEDGMEAWISMAEDEMSML 407

BLAST of Lag0021683 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 143/455 (31.43%), Postives = 228/455 (50.11%), Query Frame = 0

Query: 2   EVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDKL 61
           +++V  +E I P+SP+P     L+LS+LD + P +Y   + FY    +      +N    
Sbjct: 4   KLEVTGKEVIKPASPSP--RDRLQLSILDLYCPGIYVSTIFFYDLITESSEVFSEN---- 63

Query: 62  MATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMKL 121
              LK SLS+TL+ FY LAGRI   +I C+DEGAVF EA     + + L+  N + L   
Sbjct: 64  ---LKLSLSETLSRFYPLAGRIEGLSISCNDEGAVFTEARTDLLLPDFLRNLNTDSLSGF 123

Query: 122 LPYSVRRTDPVEECPHIVVQVNVFDCG-GIAISLCFLHKLMDATTISCFLKSWASIAANL 181
           LP       P    P + V+V  F  G G+A+S+   HK+ D  ++  F+K WA+  A  
Sbjct: 124 LPTLAAGESPA-AWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTTAKG 183

Query: 182 AYDDV--FSEMSCY--DYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKEKA 241
             +    F+E + Y      +   FP T+             S    +   +RFVF+   
Sbjct: 184 KSNSTIEFAETTIYPPPPSHMYEQFPSTD-----------SDSNITSKYVLKRFVFEPSK 243

Query: 242 ILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRMEPPL 301
           I +LK +A S+ VP PT VE +   IW+CA  ++ +       +V+  A+++R R+   +
Sbjct: 244 IAELKHKAASESVPVPTRVEAIMSLIWRCARNSSRSNLLIPRQAVMWQAMDIRLRIPSSV 303

Query: 302 -PEFSVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSEAIVKL 361
            P+  +GNL         A E++ E+ E+V   R++   +N      L G+   ++++ L
Sbjct: 304 APKDVIGNLQSGFSLKKDA-ESEFEIPEIVATFRKNKERVNEMIKESLQGNTIGQSLLSL 363

Query: 362 VGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNM--IVLLDSV 421
           + E       I + YI +S       EV+FG G PVW+G A  S T   NM  +VL+DS 
Sbjct: 364 MAETVSESTEIDR-YIMSSWCRKPFYEVDFGSGSPVWVGYA--SHTIYDNMVGVVLIDSK 423

Query: 422 SSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAI 449
             DGVE W+ L + +M + + +QE L +A++NP +
Sbjct: 424 EGDGVEAWISLPEEDMSVFVDDQELLAYAVLNPPV 433

BLAST of Lag0021683 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 8.3e-47
Identity = 139/439 (31.66%), Postives = 232/439 (52.85%), Query Frame = 0

Query: 2   EVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDKL 61
           +++ +S E I+PSSPTP  LK  K+S LDQ     + P +LFY       NP   N D  
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFY------PNPLDSNLDPA 63

Query: 62  MAT--LKTSLSKTLTHFYLLAGRI-VDTTIRCSDEGAVFVEASVSCSMSEILKE-PNNEL 121
             +  LK SLSK LTHFY LAGRI V++++ C+D G  FVEA V   +S+ ++     E 
Sbjct: 64  QTSQHLKQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEK 123

Query: 122 LMKLLP---YSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWA 181
           L + LP   Y   + +  E+ P + V+++ F+CGG AI +   HK+ D  +++ FL +W 
Sbjct: 124 LDQYLPSAAYPGGKIEVNEDVP-LAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWT 183

Query: 182 SIAANLAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKE 241
           +           +E+   ++   +  FP  +  P  P L+       +E    +RFVF +
Sbjct: 184 ATCRG------ETEIVLPNFDLAARHFPPVDNTP-SPELV------PDENVVMKRFVFDK 243

Query: 242 KAILDLKARAKS-KDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRME 301
           + I  L+A+A S  +  N + V+++  +IWK  ++      G++   V+  AVNLR RM 
Sbjct: 244 EKIGALRAQASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQAVNLRSRMN 303

Query: 302 PPLPEFSVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSEAIV 361
           PPLP +++GN+   + A   A E   +  +L+G +R S  +   ++  +L+  +G   + 
Sbjct: 304 PPLPHYAMGNIATLLFAAVDA-EWDKDFPDLIGPLRTSLEKTEDDHNHELL--KGMTCLY 363

Query: 362 KLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDSV 421
           +L  +         +L   TS   +   +++FGWGKP+   SA +++   +N  +L+D+ 
Sbjct: 364 ELEPQ---------ELLSFTSWCRLGFYDLDFGWGKPL---SACTTTFPKRNAALLMDTR 407

Query: 422 SSDGVEVWMILEKGEMELL 433
           S DGVE W+ + + EM +L
Sbjct: 424 SGDGVEAWLPMAEDEMAML 407

BLAST of Lag0021683 vs. ExPASy TrEMBL
Match: A0A5D3CZ08 (Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001080 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 8.2e-199
Identity = 356/476 (74.79%), Postives = 405/476 (85.08%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVKVLS+EN++PSSPTPPHLKT +LSLLDQFSPVLY+PL++FY  +NKDD   HQ+H++
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 120
           LMATLK+SLSKTL+HFYLLAGRIVD +IRCSDEGAVF+EA+VSCSMSEILK+PNNE LMK
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCSDEGAVFIEATVSCSMSEILKQPNNEFLMK 120

Query: 121 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASI---- 180
           L+P S R T P+EE  H++VQ NVFDCGGIAISLC LHKLMDATTI CFLK WA+I    
Sbjct: 121 LIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRRS 180

Query: 181 --AANLAYD------------------DVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEA 240
               NL  D                  D FS+M CYDYKELSSLFPQTN LPFHPRL EA
Sbjct: 181 FTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHEA 240

Query: 241 LSSGSEEESCFQRFVFKEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG- 300
           LSS  EE+S FQRFVFKEKAILDLKA+AKS DVPNPTSVEVLSGFIWKCALEAAST  G 
Sbjct: 241 LSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLGL 300

Query: 301 SQMPSVLTHAVNLRKRMEPPLPEFSVGNLFWNVVAHYTAD-EAQVELSELVGLIRQSFGE 360
           SQ+PS+LTHAVNLRKRMEPPLPEFSVGN+FWNVVAHY AD + QVELSELV LIRQSF +
Sbjct: 301 SQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADKKTQVELSELVSLIRQSFVD 360

Query: 361 INGNNIRKLVGDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIG 420
           INGN I+K+VG+EGSEA+VKLV ERN  LF IPKLYICTS R+MDL+EVNFGWGKP+WIG
Sbjct: 361 INGNYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFGWGKPIWIG 420

Query: 421 SAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           SAG+S+TTIKNMIVLLD++S+DGVE W+ILE+ EM++LL+NQEFL FAL+NPAIP+
Sbjct: 421 SAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFALLNPAIPI 476

BLAST of Lag0021683 vs. ExPASy TrEMBL
Match: A0A1S3BY96 (salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494518 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 8.2e-199
Identity = 356/476 (74.79%), Postives = 405/476 (85.08%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVKVLS+EN++PSSPTPPHLKT +LSLLDQFSPVLY+PL++FY  +NKDD   HQ+H++
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 120
           LMATLK+SLSKTL+HFYLLAGRIVD +IRCSDEGAVF+EA+VSCSMSEILK+PNNE LMK
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCSDEGAVFIEATVSCSMSEILKQPNNEFLMK 120

Query: 121 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASI---- 180
           L+P S R T P+EE  H++VQ NVFDCGGIAISLC LHKLMDATTI CFLK WA+I    
Sbjct: 121 LIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRRS 180

Query: 181 --AANLAYD------------------DVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEA 240
               NL  D                  D FS+M CYDYKELSSLFPQTN LPFHPRL EA
Sbjct: 181 FTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHEA 240

Query: 241 LSSGSEEESCFQRFVFKEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG- 300
           LSS  EE+S FQRFVFKEKAILDLKA+AKS DVPNPTSVEVLSGFIWKCALEAAST  G 
Sbjct: 241 LSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLGL 300

Query: 301 SQMPSVLTHAVNLRKRMEPPLPEFSVGNLFWNVVAHYTAD-EAQVELSELVGLIRQSFGE 360
           SQ+PS+LTHAVNLRKRMEPPLPEFSVGN+FWNVVAHY AD + QVELSELV LIRQSF +
Sbjct: 301 SQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADKKTQVELSELVSLIRQSFVD 360

Query: 361 INGNNIRKLVGDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIG 420
           INGN I+K+VG+EGSEA+VKLV ERN  LF IPKLYICTS R+MDL+EVNFGWGKP+WIG
Sbjct: 361 INGNYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFGWGKPIWIG 420

Query: 421 SAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           SAG+S+TTIKNMIVLLD++S+DGVE W+ILE+ EM++LL+NQEFL FAL+NPAIP+
Sbjct: 421 SAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFALLNPAIPI 476

BLAST of Lag0021683 vs. ExPASy TrEMBL
Match: A0A0A0KW67 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016480 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.3e-198
Identity = 357/477 (74.84%), Postives = 404/477 (84.70%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVKVLS+ENI+PSSPTPPHLKT +LSLLDQFSPVLY+PL++FY+ +NKDD   HQ+H+K
Sbjct: 1   MEVKVLSKENIMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYTMDNKDDPRHHQSHEK 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMK 120
           LMATLK+SLSKTL+HFYLLAGRIVD +I CSDEGAVF+EA+VSCSMSEILK+PNNE LMK
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSICCSDEGAVFIEATVSCSMSEILKQPNNEFLMK 120

Query: 121 LLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASI---- 180
           L+P S R T P+EE  H++VQVNVFDCGGIAISLC LHKLMDATTI CFLK WA+I    
Sbjct: 121 LVPCSERCTKPIEEYAHVIVQVNVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINKRS 180

Query: 181 --AANLAYD-------------------DVFSEMSCYDYKELSSLFPQTNLLPFHPRLLE 240
               NL  D                   D FS+M CYDYKELSSLFPQTN LPFHPRL E
Sbjct: 181 FATTNLTIDNGASELFTPPSSMTNDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 ALSSGSEEESCFQRFVFKEKAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG 300
           ALSS  EE+S FQRFVFKEKAILDLKA+AKS DVPNPTSVEVLSGFIWKCALEAAST  G
Sbjct: 241 ALSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLG 300

Query: 301 -SQMPSVLTHAVNLRKRMEPPLPEFSVGNLFWNVVAHYTAD-EAQVELSELVGLIRQSFG 360
            SQ+PS+LTHAVNLRKRMEPPLPEFSVGN+FWNVVAHY AD + QVELSELV LIRQSF 
Sbjct: 301 LSQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADKKTQVELSELVSLIRQSFV 360

Query: 361 EINGNNIRKLVGDEGSEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWI 420
           +INGN I+++VG+EGSEA+VKLV ERN  LF IPKLYICTS R+MDL+EVNFGWGKPVWI
Sbjct: 361 DINGNYIKRIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFGWGKPVWI 420

Query: 421 GSAGSSSTTIKNMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           GSAG+S+TTIKNMIVLLD++S+DGVE W+ILE+ EM++LLQNQEF  FAL+NP IP+
Sbjct: 421 GSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLQNQEFFNFALLNPPIPI 477

BLAST of Lag0021683 vs. ExPASy TrEMBL
Match: A0A6J1CAD9 (BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 5.0e-148
Identity = 273/460 (59.35%), Postives = 345/460 (75.00%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           MEVK+LSRE I+PSSPTPPHLKT +LSL+DQ SP LY PLL FY   NK D  TH N  K
Sbjct: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCS-DEGAVFVEASVSCSMSEILKEPNNELLM 120
            +ATLK SLSKTLT+F+LLAGRI +T+IRCS DEGAVF+EA+V+C++S+ILKEPNNE+L 
Sbjct: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120

Query: 121 KLLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAAN 180
           KLLP S++ T P EE   IV Q N+F+CGGI ISLC LHKLMD  + SCFLKSWASI   
Sbjct: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180

Query: 181 LAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEA---LSSGSEEESCFQRFVFKEKA 240
            + D+    + C +YK  SSLFPQTNLL FH ++ E    + S S+   C QRFVF  KA
Sbjct: 181 -SLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKA 240

Query: 241 ILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISG----SQMPSVLTHAVNLRKRM 300
           I DLKA+AKS +VPNPTSVE L+GFIW CA++  +T  G    SQ PS L+H VNLRKRM
Sbjct: 241 ISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRM 300

Query: 301 EPPLPEFSVGNLFWNVVAHYTAD--EAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSE 360
           EPPLPE S+GN+ W +VAHY +   E QVEL +LV L+RQSF +IN + I+ L+G++G+ 
Sbjct: 301 EPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNR 360

Query: 361 AIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLL 420
           A+++L+GE NK    +P  Y  TS R+MDLNEV+FGWGKP+WIG AG+S+   K+ I+LL
Sbjct: 361 AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLL 420

Query: 421 DSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           D+ S DG+E WM+L++ +M+LL QN +FLEFA +NP+I +
Sbjct: 421 DTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 458

BLAST of Lag0021683 vs. ExPASy TrEMBL
Match: A0A6J1GVT5 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111458004 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 3.1e-137
Identity = 269/453 (59.38%), Postives = 330/453 (72.85%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYS-TNNKDDNPTHQNHD 60
           MEVKVLSRE IIPSSPTPPHLKT +LSLLDQ SP+LY+PLL FY  T   D NPT     
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKTFELSLLDQLSPMLYTPLLFFYPITKTYDPNPT----- 60

Query: 61  KLMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLM 120
             MATLK SLS+TLT FYLLAGRIV  +I CSD+GAVF+EASVSC+MS ILK+PNNE+L 
Sbjct: 61  --MATLKDSLSQTLTRFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVLR 120

Query: 121 KLLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAAN 180
           KLLP S+  ++P EE P +VVQ N+F CGGIAISLC LHKLMD  T + FL SWAS   N
Sbjct: 121 KLLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWAS--TN 180

Query: 181 LAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKEKAILD 240
               D  S M CYDYK  SSLFPQTNLLPFH    + L+  +  + C +RFVF  K++L+
Sbjct: 181 KGSLDSSSNMICYDYKAFSSLFPQTNLLPFH----QKLAKPANRKRCLRRFVFGSKSLLN 240

Query: 241 LKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRMEPPLPEF 300
           LKA+A+S+ VPNP+ VE L+GFIWK A+ AA      Q  S L+H VNLR RM+P LPE 
Sbjct: 241 LKAKAESRAVPNPSCVEALTGFIWKHAMMAAD----CQGSSTLSHVVNLRGRMKPALPEV 300

Query: 301 SVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNI-RKLVG-DEGSEAIVKLVG 360
           SVGN+ W  VAH +A E ++EL  LVGL+RQSF EIN + I R L+G D+G EA++K VG
Sbjct: 301 SVGNIMWGAVAHSSAME-RLELRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVG 360

Query: 361 ERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDSVSSDG 420
           E NK  +G+   Y CTS R+M LNEV+FGWG+P WI  A  ++  + N+IVL+D V  DG
Sbjct: 361 EMNK--WGMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDG 420

Query: 421 VEVWMILEKGEMELLLQNQEFLEFALVNPAIPL 451
           +E W++L++ EME+L QN +FLEFAL+NP+I L
Sbjct: 421 IEAWILLDEKEMEILEQNPQFLEFALLNPSIYL 433

BLAST of Lag0021683 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-76
Identity = 174/454 (38.33%), Postives = 262/454 (57.71%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           M V V+SR+ I PSSPTP HLK  KLSLL+Q  P ++ P++ FYS NN    PT Q    
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANN-SIKPTEQ---- 60

Query: 61  LMATLKTSLSKTLTHFYLLAGRIV-DTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLM 120
            +  LK SLS+TLTHFY LAGR+  + +I C+D GA F+EA V+  +S +L EP+++ L 
Sbjct: 61  -LQMLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQ 120

Query: 121 KLLPYSVRRTDPVEECPHIVV-QVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAA 180
           +L+P SV   D +E    +++ Q + F+CG ++I +C  HKL DAT+I  F+KSWA+I++
Sbjct: 121 QLIPTSV---DSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISS 180

Query: 181 NLAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKEKAIL 240
             +   + + +      +   +FP  N     P  +         ++  +RF+F   +I 
Sbjct: 181 RGSIKTIGAPVF-----DTVKIFPPGNFSETSPAPV-VEPEIMMNQTLSKRFIFDSSSIQ 240

Query: 241 DLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRMEPPLPE 300
            L+A+A S +V  PT VE +S  IWK A++A  T+SG+  PS+L ++V+LR R+ PP  +
Sbjct: 241 ALQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTK 300

Query: 301 FSVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSEAIV----K 360
            S+GNL     A       Q +L  LV  IR++       +I KLVG+  +  I+    K
Sbjct: 301 NSIGNLVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQK 360

Query: 361 LVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDSVS 420
             G+   +  G    YI +S     L E +FGWGKPVW+   G  S   KN++ LLD+  
Sbjct: 361 EAGD--MIASGDFDFYIFSSACRFGLYETDFGWGKPVWV---GFPSVRQKNIVTLLDTKE 420

Query: 421 SDGVEVWMILEKGEMELLLQNQEFLEFALVNPAI 449
           + G+E W+ L + EM L  Q++E L+FA +NP++
Sbjct: 421 AGGIEAWVNLNEQEMNLFEQDRELLQFASLNPSV 434

BLAST of Lag0021683 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.1e-62
Identity = 160/466 (34.33%), Postives = 251/466 (53.86%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           M+V+ +S+E I PSSPTP +L+TL+LS+ D   P +Y+   LFY T N   +  H +H  
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY-TKNDLISQEHTSH-- 65

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASV-SCSMSEILKEPNNELLM 120
               LKTSLS+TLT FY LAGRI   T+ C+DEGA+FV+A V +C ++E LK P+ + L 
Sbjct: 66  ---KLKTSLSETLTKFYPLAGRITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQ 125

Query: 121 KLLPYSVRRTDPVEEC--PHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIA 180
           +LLP  V     V     P ++V+   F CGG+AI +C  HK+ DA +IS F++SWA+ A
Sbjct: 126 QLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 185

Query: 181 ANLAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEE-----SCFQRFVF 240
                ++  + M        S +F   N   F+P   EA    ++E+     S  +RFVF
Sbjct: 186 RG---ENDAAAME-------SPVFAGAN---FYPPANEAFKLPADEQAGKRSSITKRFVF 245

Query: 241 KEKAILDLKARAKSKD-VPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKR 300
           +   + DL+ +A S++ V  PT VE ++  IWKC + ++ T +      VL    NLR +
Sbjct: 246 EASKVEDLRTKAASEETVDQPTRVESVTALIWKCFVASSKTTTCDH--KVLVQLANLRSK 305

Query: 301 MEPPLPEFSVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSEA 360
           +   L E S+GNL ++ V        +V++ E V  +R+   E+       ++ + GS  
Sbjct: 306 IPSLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELG----TVILDEGGSSD 365

Query: 361 IVKLVGER---------NKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTT 420
              ++G +         ++L +   + Y  +S   + L E +FGW  PVW+   G+ S  
Sbjct: 366 SSSMIGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWV--VGNVSPV 425

Query: 421 IKNMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAI 449
           + N+ +L+DS    G+E ++ L +  M    QN E L FA +NP++
Sbjct: 426 LGNLAMLIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATMNPSV 444

BLAST of Lag0021683 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-61
Identity = 159/464 (34.27%), Postives = 258/464 (55.60%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDD--NPTHQNH 60
           M+V+ +++E I PSS TP  L+TL+LS+ D   P +Y+   LFY+   KDD  +P   +H
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYT---KDDLISPEQSSH 63

Query: 61  DKLMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASV-SCSMSEILKEPNNEL 120
                 LKTSL++TLT FY LAGRI   TI C+DEGAVFV+A V +  +S+ L+ P+ + 
Sbjct: 64  -----KLKTSLAETLTKFYPLAGRIKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKT 123

Query: 121 LMKLLPYSVRRTDPVEEC---PHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWA 180
           L +LLP  V   DP E     P ++V+   F CGG+AI LC  HK+ DAT+IS F++SWA
Sbjct: 124 LQQLLPLDV-IDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWA 183

Query: 181 SIAANLAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKE 240
           ++A   A D V   +        ++ +P  N L   P       + ++     +RFVF  
Sbjct: 184 AMARGEAGDIVADPVFA-----AANFYPPANELFKFP----VDENANKISQITKRFVFSA 243

Query: 241 KAILDLKARAKSKD-VPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRME 300
             + +L+ +A S+D V  P  VE ++  +WK  + AAS+ + +    VL    NLR ++ 
Sbjct: 244 SKLEELRTKAASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNLRPKIP 303

Query: 301 PPLPEFSVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGDEGSEAIV 360
             L E  +GN+ ++ V    + + ++++ + V  +R+      G++++ ++ +EG  +  
Sbjct: 304 SLLAESLIGNIMFSSVVLGISQQEEIKIEKAVRDLRK-----QGDDLQNVIKEEGGSSSA 363

Query: 361 KLVGER---------NKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIK 420
            ++G +         +KL +   + Y  +S   + L E +FGWG PVWI  AG+ +  ++
Sbjct: 364 -MIGSKLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWI--AGNVAPMLE 423

Query: 421 NMIVLLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAI 449
           N+ +L+DS    G+E ++ L +  M  L QN E L FA VNP++
Sbjct: 424 NVTMLIDSKDGQGIEAFVTLPEENMLSLEQNTELLAFASVNPSV 441

BLAST of Lag0021683 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-60
Identity = 160/460 (34.78%), Postives = 246/460 (53.48%), Query Frame = 0

Query: 3   VKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDKLM 62
           V++LSRE + PSSPTP   + L LSLLD  S  +Y+  LLFY+ +    N    + ++  
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAAD--PQNLLGFSTEETS 64

Query: 63  ATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASVSCSMSEILKEPNNELLMKLL 122
             LK SLSKTL  FY LAGRI+ + + C+DEGAVF+EA V   +SE LK P  E L  L+
Sbjct: 65  LKLKKSLSKTLPIFYPLAGRIIGSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLELLI 124

Query: 123 PYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAANLAY 182
           P   +  + V   P +++Q N F CGG+ I++C  HK+ DAT+++ F++ WA  +  L  
Sbjct: 125 PVEAKSREAV-TWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGLGI 184

Query: 183 DDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGS-------EEESCF-QRFVFKE 242
                           +L P        P+ L+ L S         EE SC  +RFVF  
Sbjct: 185 ----------------TLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDA 244

Query: 243 KAILDLKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRMEP 302
             I  L+A+A    V NPT VE ++   W+C  + +   S +   SVL   VNLR +++ 
Sbjct: 245 SKIKKLRAKASRNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRGKVD- 304

Query: 303 PLPEFSVGNLFWNVVAHYTADEAQVE-LSELVGLIRQSFGEINGNNIRKLVGD-----EG 362
            L E ++GN+   ++     +EA +E + ++V  IR++  EI   N +++        E 
Sbjct: 305 SLCENTIGNMLSLMI--LKNEEAAIERIQDVVDEIRRA-KEIFSLNCKEMSKSSSRIFEL 364

Query: 363 SEAIVKLVGERNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIV 422
            E I K+ G  N++      L++  S   + L + +FGWGKPVW+   G+S    KN+++
Sbjct: 365 LEEIGKVYGRGNEM-----DLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSH--FKNLML 424

Query: 423 LLDSVSSDGVEVWMILEKGEMELLLQNQEFLEFALVNPAI 449
           L+D+   +G+E W+ L + +M L   +QE LE A +NP +
Sbjct: 425 LIDTKDGEGIEAWITLTEEQMSLFECDQELLESASLNPPV 434

BLAST of Lag0021683 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.8e-57
Identity = 159/454 (35.02%), Postives = 240/454 (52.86%), Query Frame = 0

Query: 1   MEVKVLSRENIIPSSPTPPHLKTLKLSLLDQFSPVLYSPLLLFYSTNNKDDNPTHQNHDK 60
           M+V+ + +E I PS+ TP  L TL+LS++D   P +Y+   LFY+   KDD     + ++
Sbjct: 4   MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFYT---KDD---LISQEQ 63

Query: 61  LMATLKTSLSKTLTHFYLLAGRIVDTTIRCSDEGAVFVEASV-SCSMSEILKEPNNELLM 120
              TLKTSLS+ LT F+ LAGR+   TI+ +DEGAVFVEA V +C +S  L+ P+ E L 
Sbjct: 64  TSHTLKTSLSEILTKFHPLAGRVNGVTIKSTDEGAVFVEARVDNCDLSGFLRSPDTESLK 123

Query: 121 KLLPYSVRRTDPVEECPHIVVQVNVFDCGGIAISLCFLHKLMDATTISCFLKSWASIAAN 180
           +LLP      +P    P ++V+   F CGG+AI LC  H+L DA ++S FL++WA+ A  
Sbjct: 124 QLLPVD---DEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARG 183

Query: 181 LAYDDVFSEMSCYDYKELSSLFPQTNLLPFHPRLLEALSSGSEEESCFQRFVFKEKAILD 240
            + D V S   C            T L P     ++         S  +RFVF    I +
Sbjct: 184 ES-DLVASPDFC-----------STKLYPAANEAIKIPGEVVNRTSVTKRFVFVASKIEE 243

Query: 241 LKARAKSKDVPNPTSVEVLSGFIWKCALEAASTISGSQMPSVLTHAVNLRKRMEPPLPEF 300
           L+ +  S  VP PT V+ ++  IWKC + A+   + +     L    NLR ++   L E 
Sbjct: 244 LRNKVASDVVPRPTRVQSVTSLIWKCVVTAS---TDTIREKALFQPANLRTKIPSLLSEN 303

Query: 301 SVGNLFWNVVAHYTADEAQVELSELVGLIRQSFGEINGNNIRKLVGD-EGSEAIV--KLV 360
            +GNL +N +      +A +++ E V  +++   +++G     LV D EGS   +  +L 
Sbjct: 304 QIGNLLFNSL--ILDGKAGMDIVETVKELQKRAEDLSG-----LVQDEEGSSMTIGWRLF 363

Query: 361 GE--RNKLLFGIPKLYICTSCRHMDLNEVNFGWGKPVWIGSAGSSSTTIKNMIVLLDSVS 420
           GE   +K       ++  TS   + L +  FGWG PVW+  AGS S  +  + VL+DS  
Sbjct: 364 GEIINSKFNREDHDMHSVTSWCKIPLYDACFGWGSPVWV--AGSVSPNLDYVTVLIDSKD 423

Query: 421 SDGVEVWMILEKGEMELLLQNQEFLEFALVNPAI 449
             G+E W+ L +  M LL Q+ E L FA  NP++
Sbjct: 424 GQGIEAWVTLPQENMLLLEQSTELLAFASPNPSV 424