Lag0021569 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021569
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein RALF-like 32
Locationchr7: 9283227 .. 9283637 (-)
RNA-Seq ExpressionLag0021569
SyntenyLag0021569
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGAAGTAACTAAGAACACCAAGAGCATGAAAGAACGAAGCTCAAGCCACCAAAGCCTCCGCGTCCTCCTCCTCCTCGGCCATCTATATCTACTAATTTTTGACAACATGGTCGTCGTCGCACTCGGCAGCTCGATGGTCCCGTGCAACGGTACGATAGCAGAGTGCTACGAAGAGGAAGAGATGTTAATGGAGTCGGAGATAAGTCGAAGGTTTCTCGAACAACAAAGAAAGAAAATAACGTATCCTGCTCTGAGACCTGACAAACCTGCTTGTGATAATGGAGCCGTAGGTCAACCTTATGGCAAAAGCTGTGCTCCTTCTGTGGCTAATAAATATAACACAGGATCATGTGGTTCAGTATACAACAGAGGATGCCCTTCAAAAAATACTCCGGCGCGGCCTTAG

mRNA sequence

ATGCAAGAAGTAACTAAGAACACCAAGAGCATGAAAGAACGAAGCTCAAGCCACCAAAGCCTCCGCGTCCTCCTCCTCCTCGGCCATCTATATCTACTAATTTTTGACAACATGGTCGTCGTCGCACTCGGCAGCTCGATGGTCCCGTGCAACGGTACGATAGCAGAGTGCTACGAAGAGGAAGAGATGTTAATGGAGTCGGAGATAAGTCGAAGGTTTCTCGAACAACAAAGAAAGAAAATAACGTATCCTGCTCTGAGACCTGACAAACCTGCTTGTGATAATGGAGCCGTAGGTCAACCTTATGGCAAAAGCTGTGCTCCTTCTGTGGCTAATAAATATAACACAGGATCATGTGGTTCAGTATACAACAGAGGATGCCCTTCAAAAAATACTCCGGCGCGGCCTTAG

Coding sequence (CDS)

ATGCAAGAAGTAACTAAGAACACCAAGAGCATGAAAGAACGAAGCTCAAGCCACCAAAGCCTCCGCGTCCTCCTCCTCCTCGGCCATCTATATCTACTAATTTTTGACAACATGGTCGTCGTCGCACTCGGCAGCTCGATGGTCCCGTGCAACGGTACGATAGCAGAGTGCTACGAAGAGGAAGAGATGTTAATGGAGTCGGAGATAAGTCGAAGGTTTCTCGAACAACAAAGAAAGAAAATAACGTATCCTGCTCTGAGACCTGACAAACCTGCTTGTGATAATGGAGCCGTAGGTCAACCTTATGGCAAAAGCTGTGCTCCTTCTGTGGCTAATAAATATAACACAGGATCATGTGGTTCAGTATACAACAGAGGATGCCCTTCAAAAAATACTCCGGCGCGGCCTTAG

Protein sequence

MQEVTKNTKSMKERSSSHQSLRVLLLLGHLYLLIFDNMVVVALGSSMVPCNGTIAECYEEEEMLMESEISRRFLEQQRKKITYPALRPDKPACDNGAVGQPYGKSCAPSVANKYNTGSCGSVYNRGCPSKNTPARP
Homology
BLAST of Lag0021569 vs. NCBI nr
Match: XP_004147646.3 (protein RALF-like 32 [Cucumis sativus])

HSP 1 Score: 104.0 bits (258), Expect = 1.0e-18
Identity = 63/122 (51.64%), Postives = 80/122 (65.57%), Query Frame = 0

Query: 5   TKNTKSMKERSSSHQSLRV-LLLLGHLYLLIFDNMVVVALGSSMVPCNGTIAECYEEEEM 64
           ++N  + K  SS   SL+   LLL  L+L +F ++ +V   SS   CNG+IAEC  EEEM
Sbjct: 3   SQNNNTRKRISSKPMSLQTCFLLLLFLFLFLFQDLCLVR-ASSTHSCNGSIAECANEEEM 62

Query: 65  LMESEISRRFLEQQRKKITYPALRPDKPACDNGAVGQPYGK--SCAPSVANKYNTGSCGS 124
           LMESEI+RRFLEQQ+K I+  AL+ D PACD  + GQPY +  SCAP  AN YN G C  
Sbjct: 63  LMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRG-CSK 122

BLAST of Lag0021569 vs. NCBI nr
Match: XP_022138044.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 102.8 bits (255), Expect = 2.3e-18
Identity = 60/109 (55.05%), Postives = 75/109 (68.81%), Query Frame = 0

Query: 17  SHQSLRVLLLLGHLYLLIFDNMVVVALGSSMVPCNGTIAECYEEEEMLMESEISRRFLEQ 76
           +H+ + VL+L+  L  L   N V   LG+SM PCNG+IAECY EEEMLMESEISRRFLEQ
Sbjct: 13  THRLVHVLILV--LLCLPSFNNVFTVLGNSMTPCNGSIAECYVEEEMLMESEISRRFLEQ 72

Query: 77  QRKKITYPALRPDKPACDNGAVGQPYGK--SCAPSVANKYNTGSCGSVY 124
           Q+K I+Y  L+ D+PAC  G  G+PY K  SC P  +N Y+ G C  +Y
Sbjct: 73  QKKYISYDTLKKDQPACQGGK-GKPYTKSESCLPPPSNPYDRG-CKPIY 117

BLAST of Lag0021569 vs. NCBI nr
Match: KAG6582109.1 (Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018526.1 Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 99.8 bits (247), Expect = 2.0e-17
Identity = 64/114 (56.14%), Postives = 75/114 (65.79%), Query Frame = 0

Query: 13  ERSSSHQSLRVLLLLGHLYLLIFDN-MVVVALGSSMVPCNGTIAECYEEEEMLMESEISR 72
           E SSSH SL   L+LG LYLLIF N +VVVA+ S + PCNG+IAECY+E E LMESEISR
Sbjct: 2   EGSSSHSSLN--LVLGLLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISR 61

Query: 73  RFLEQQRKKITYPALRPDKPACDNGAVGQPYGKSCAPSVANKYNTGSCGSVYNR 126
           RFL +  K +TY  L  +K  C NG  G+PY   C P  AN Y+   C   YNR
Sbjct: 62  RFLGRP-KDLTYEFLDKNKAPCANGR-GKPYRGKCQPREANPYDR-ECSGTYNR 110

BLAST of Lag0021569 vs. NCBI nr
Match: XP_023528073.1 (protein RALF-like 32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 97.1 bits (240), Expect = 1.3e-16
Identity = 55/96 (57.29%), Postives = 66/96 (68.75%), Query Frame = 0

Query: 30  LYLLIFDNMVVVALGSSMVPCNGTIAECYEEEEMLMESEISRRFLEQQRKKITYPALRPD 89
           L+LL+  ++ +V   SS   CNG+IAEC  EEE LMESEISRRFLEQQ+K I+  AL+ D
Sbjct: 12  LFLLLVQDLCLVR-ASSKHSCNGSIAECGGEEETLMESEISRRFLEQQKKYISIGALKKD 71

Query: 90  KPACDNGAVGQPYGK--SCAPSVANKYNTGSCGSVY 124
            PACD G  GQPY +  SCAP  AN YN G C  +Y
Sbjct: 72  HPACDGGGGGQPYTRSGSCAPPPANPYNRG-CSKIY 105

BLAST of Lag0021569 vs. NCBI nr
Match: XP_022138261.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 96.7 bits (239), Expect = 1.7e-16
Identity = 53/94 (56.38%), Postives = 67/94 (71.28%), Query Frame = 0

Query: 32  LLIFDNMVVVALGSSMVPCNGTIAECYEEEEMLMESEISRRFLEQQRKKITYPALRPDKP 91
           LL F+N V + +GSSM PCNG+I+ECY EEE L ESEISRRFLEQQ+K I+Y A + D+P
Sbjct: 21  LLSFNNAVTI-VGSSMTPCNGSISECYAEEETLXESEISRRFLEQQKKYISYDARKKDQP 80

Query: 92  ACDNGAVGQPY--GKSCAPSVANKYNTGSCGSVY 124
           AC  G  G+PY   +SC P  +N Y+ G C  +Y
Sbjct: 81  AC-KGGKGKPYTESESCLPPPSNPYDRG-CSLIY 111

BLAST of Lag0021569 vs. ExPASy Swiss-Prot
Match: O23262 (Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 38/92 (41.30%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 40  VVALGSSMVPCNGTIAEC-----YEEEEMLMESEISRRFLEQQRKKITYPALRPDKPACD 99
           V +  SS   CNG++AEC      EE  ++MES  S+R  E+Q  K++Y ALR ++PACD
Sbjct: 24  VTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACD 83

Query: 100 NGAVGQPYGKSCAPSVANKYNTGSCGSVYNRG 127
            G  G+ Y   C P  +N Y+ G C   Y  G
Sbjct: 84  GGKRGESYSTQCLPPPSNPYSRG-CSKHYRCG 114

BLAST of Lag0021569 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.3e-05
Identity = 34/85 (40.00%), Postives = 46/85 (54.12%), Query Frame = 0

Query: 36  DNMVVVALGSSMV--PCNGTIAECY-EEEEMLMESEISRRFLEQQRKKITYPALRPDKPA 95
           D++  V  GSS +   C G+IAEC  EEEEM  +S+ISRR L  Q+K I+Y A+R +   
Sbjct: 29  DSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNSVP 88

Query: 96  ----------CDNGAVGQPYGKSCA 108
                     C  GA   PY + C+
Sbjct: 89  CSRRGASYYNCQRGAQANPYSRGCS 112

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147646.31.0e-1851.64protein RALF-like 32 [Cucumis sativus][more]
XP_022138044.12.3e-1855.05protein RALF-like 32 [Momordica charantia][more]
KAG6582109.12.0e-1756.14Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018526... [more]
XP_023528073.11.3e-1657.29protein RALF-like 32 [Cucurbita pepo subsp. pepo][more]
XP_022138261.11.7e-1656.38protein RALF-like 32 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O232621.1e-1041.30Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1[more]
Q9MA625.3e-0540.00Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 61..119
e-value: 4.9E-13
score: 49.8
NoneNo IPR availablePANTHERPTHR33136:SF4PROTEIN RALF-LIKE 32coord: 21..125
NoneNo IPR availablePANTHERPTHR33136RAPID ALKALINIZATION FACTOR-LIKEcoord: 21..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021569.1Lag0021569.1mRNA