Lag0021557 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021557
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMLP-like protein 43
Locationchr7: 9133217 .. 9133993 (-)
RNA-Seq ExpressionLag0021557
SyntenyLag0021557
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATGTAAAAGGAAAGCTTCACTGATTTTCCATCAAGGGAGAATTGGAGCATTGAGGCGCTGGAGATTTTATTTGAATGATTTTTGGAGCCTTGATTTGGGTGATTTCTCCATAATTGTGAGAATTAGCTAATGTTGTGTTGTCATGAGCACATTTTTCCAACTGTGCTTTTGTGATTAATGTGGTTGTCTGTGGGTGTATTTGTGAGCTGCTATTATTTCTTTCAATTTCAGGCGAGACCTTACCTCCTCACTAAAATCTCTCCCGAAAATTTACACAGCTCTGAGTTACTAAAAGGCGAATGGGGCAAAGTTGGCTCTATTATCTATTGGACCTATACGCTCGGTTTGTTTCTAATTTTCGAATCATCTCTTTGAATTTTCATTTTCTTTCTAAAAACCATTGCATTGATGTTATATTTACCTGTTACTGTAGATTAAATTCATAGGTGGAAAGCCTTCGGTAGTCAAACACAAAGTTGAAGCTGTTGATGAAGAGAAAAATTTGATCACTTTCACATGGATAGAAGGAACCCTTTTGGAGTATTACAAAAAGTTTCAAGTTACAGTTCAATGTGTTCAAAAACATATGGGAAGCGTGGTTCGTTGGATTACTGAATTTGAGATATTGCGCGTTGAGATTCCATATTTACGTAACTTGCCTCAGTTCTATGGTTCGATCTCGAAAGACATTGAAGCTTACCTTAGGCAAGGTAACCAAGGACCATATGAAGATTTTGCAGACGATGATGTTGCATCAACTTCCAAGGCATGA

mRNA sequence

ATGAAATGTAAAAGGAAAGCTTCACTGATTTTCCATCAAGGGAGAATTGGAGCATTGAGGCGCTGGAGATTTTATTTGAATGATTTTTGGAGCCTTGATTTGGGTGATTTCTCCATAATTGCGAGACCTTACCTCCTCACTAAAATCTCTCCCGAAAATTTACACAGCTCTGAGTTACTAAAAGGCGAATGGGGCAAAGTTGGCTCTATTATCTATTGGACCTATACGCTCGGTGGAAAGCCTTCGGTAGTCAAACACAAAGTTGAAGCTGTTGATGAAGAGAAAAATTTGATCACTTTCACATGGATAGAAGGAACCCTTTTGGAGTATTACAAAAAGTTTCAAGTTACAGTTCAATGTGTTCAAAAACATATGGGAAGCGTGGTTCGTTGGATTACTGAATTTGAGATATTGCGCGTTGAGATTCCATATTTACGTAACTTGCCTCAGTTCTATGGTTCGATCTCGAAAGACATTGAAGCTTACCTTAGGCAAGGTAACCAAGGACCATATGAAGATTTTGCAGACGATGATGTTGCATCAACTTCCAAGGCATGA

Coding sequence (CDS)

ATGAAATGTAAAAGGAAAGCTTCACTGATTTTCCATCAAGGGAGAATTGGAGCATTGAGGCGCTGGAGATTTTATTTGAATGATTTTTGGAGCCTTGATTTGGGTGATTTCTCCATAATTGCGAGACCTTACCTCCTCACTAAAATCTCTCCCGAAAATTTACACAGCTCTGAGTTACTAAAAGGCGAATGGGGCAAAGTTGGCTCTATTATCTATTGGACCTATACGCTCGGTGGAAAGCCTTCGGTAGTCAAACACAAAGTTGAAGCTGTTGATGAAGAGAAAAATTTGATCACTTTCACATGGATAGAAGGAACCCTTTTGGAGTATTACAAAAAGTTTCAAGTTACAGTTCAATGTGTTCAAAAACATATGGGAAGCGTGGTTCGTTGGATTACTGAATTTGAGATATTGCGCGTTGAGATTCCATATTTACGTAACTTGCCTCAGTTCTATGGTTCGATCTCGAAAGACATTGAAGCTTACCTTAGGCAAGGTAACCAAGGACCATATGAAGATTTTGCAGACGATGATGTTGCATCAACTTCCAAGGCATGA

Protein sequence

MKCKRKASLIFHQGRIGALRRWRFYLNDFWSLDLGDFSIIARPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFTWIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEAYLRQGNQGPYEDFADDDVASTSKA
Homology
BLAST of Lag0021557 vs. NCBI nr
Match: XP_022956029.1 (MLP-like protein 43 [Cucurbita moschata])

HSP 1 Score: 136.0 bits (341), Expect = 3.4e-28
Identity = 62/129 (48.06%), Postives = 88/129 (68.22%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           R + ++ +S + +H  +LL+GEWG+VGSII W Y   GK  V+K ++EAVDEE N ITFT
Sbjct: 27  RTHHISNVSTDKVHGVDLLEGEWGEVGSIICWRYFHDGKARVLKERIEAVDEENNSITFT 86

Query: 102 WIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEA 161
            IEG L E YK F+  +QC+ K  GSV+ W+ E+E L  EIP   ++ QF   +SKDI+A
Sbjct: 87  VIEGDLTEKYKSFKFNIQCIPKEKGSVIHWVLEYEKLHDEIPDSHSMLQFCIDVSKDIDA 146

Query: 162 YLRQGNQGP 171
           +L +G++ P
Sbjct: 147 HLMEGSEEP 155

BLAST of Lag0021557 vs. NCBI nr
Match: XP_023526913.1 (MLP-like protein 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 135.6 bits (340), Expect = 4.4e-28
Identity = 61/132 (46.21%), Postives = 89/132 (67.42%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           R + ++ +S + +H  +LL+GEWG+VGSII W Y   GK  V K ++EAVD++ N ITFT
Sbjct: 27  RTHRISNVSTDKVHGVDLLEGEWGEVGSIICWRYFHDGKARVTKERIEAVDKQNNSITFT 86

Query: 102 WIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEA 161
            IEG L E YK F+  +QC+ K  GSV+ W+ E+E L  EIP   ++ QF   +SKDI+A
Sbjct: 87  VIEGDLTEKYKSFKFNIQCIPKEKGSVIHWVLEYEKLHDEIPDSHSMLQFCVEMSKDIDA 146

Query: 162 YLRQGNQGPYED 174
           +L +G++ PY +
Sbjct: 147 HLMEGSEEPYHN 158

BLAST of Lag0021557 vs. NCBI nr
Match: XP_022979964.1 (MLP-like protein 43 [Cucurbita maxima])

HSP 1 Score: 135.6 bits (340), Expect = 4.4e-28
Identity = 62/129 (48.06%), Postives = 86/129 (66.67%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           R + ++ +S + +H  +LL+GEWGKVGSII W Y   GK  V K ++EAVDEE N ITF 
Sbjct: 27  RTHHISNVSADKVHGIDLLEGEWGKVGSIICWRYFHDGKARVAKERIEAVDEENNSITFA 86

Query: 102 WIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEA 161
            IEG L E YK F+  +QC+ K  GSV+ W+ E+E L  EIP   ++ QF   +SKDI+A
Sbjct: 87  VIEGDLTEKYKSFKFNIQCIPKEKGSVIHWVLEYEKLHDEIPDSHSMLQFCVDVSKDIDA 146

Query: 162 YLRQGNQGP 171
           +L +G++ P
Sbjct: 147 HLMEGSEEP 155

BLAST of Lag0021557 vs. NCBI nr
Match: XP_022944436.1 (MLP-like protein 34 [Cucurbita moschata])

HSP 1 Score: 134.8 bits (338), Expect = 7.6e-28
Identity = 60/129 (46.51%), Postives = 87/129 (67.44%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           RP+ ++ +S + +   E+ +GEWG+VGSI+YW Y   GKP V K  +EAVDEE N+I+F 
Sbjct: 27  RPHHISNVSSDKIQGCEIHEGEWGQVGSIVYWNYVHDGKPCVAKEIIEAVDEENNMISFK 86

Query: 102 WIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEA 161
            IEG LLE YK F++T+ C+ +  GSV+ W  E+E L  E+P   +L QF   +SKDIEA
Sbjct: 87  VIEGDLLEEYKSFKLTIHCLPRDKGSVIHWTLEYEKLHDEVPDSHSLLQFCVDVSKDIEA 146

Query: 162 YLRQGNQGP 171
           +L + ++ P
Sbjct: 147 HLMEDDKEP 155

BLAST of Lag0021557 vs. NCBI nr
Match: XP_023526175.1 (MLP-like protein 43 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 134.4 bits (337), Expect = 9.9e-28
Identity = 61/129 (47.29%), Postives = 87/129 (67.44%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           R + ++ +S + +H  +LL+GEWG+VGSII W Y   GK   +K ++EAVDEE N ITFT
Sbjct: 27  RTHHISNVSTDKVHGIDLLEGEWGEVGSIICWRYFHDGKARALKERIEAVDEENNSITFT 86

Query: 102 WIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEA 161
            IEG L E YK F+  +QC+ K  GSV+ W+ E+E L  EIP   ++ QF   +SKDI+A
Sbjct: 87  VIEGDLTEKYKSFKFNIQCIPKEKGSVIHWVLEYEKLHDEIPDSHSMLQFCIDVSKDIDA 146

Query: 162 YLRQGNQGP 171
           +L +G++ P
Sbjct: 147 HLMEGSEEP 155

BLAST of Lag0021557 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.6e-26
Identity = 55/124 (44.35%), Postives = 81/124 (65.32%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           +P+ ++K +P N+ S +L +G+WG VGSI++W Y   G+  V K ++EAVD EKNLITF 
Sbjct: 189 KPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEAKVAKERIEAVDPEKNLITFR 248

Query: 102 WIEGTLLEYYKKFQVTVQCVQKH--MGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDI 161
            IEG L++ YK F +T+Q   KH   GSVV W  E+E +  E+ +   L QF   +SK+I
Sbjct: 249 VIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKINEEVAHPETLLQFAVEVSKEI 308

Query: 162 EAYL 164
           + +L
Sbjct: 309 DEHL 312

BLAST of Lag0021557 vs. ExPASy Swiss-Prot
Match: Q9SSK5 (MLP-like protein 43 OS=Arabidopsis thaliana OX=3702 GN=MLP43 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 6.2e-25
Identity = 56/124 (45.16%), Postives = 79/124 (63.71%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           RP+ ++K +P+ +H  EL +G+WGKVGSI+ W Y   GK +V K+K+EAVD EKNLITF 
Sbjct: 31  RPHHVSKATPDKIHGCELHEGDWGKVGSIVIWKYVHDGKLTVGKNKIEAVDPEKNLITFK 90

Query: 102 WIEGTLLEYYKKFQVTVQCVQK--HMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDI 161
            +EG L+  YK F  T+Q   K    GS+  W  E+E +  E+ +   L QF   ISK+I
Sbjct: 91  VLEGDLMNEYKSFAFTLQVTPKQGESGSIAHWHLEYEKISEEVAHPETLLQFCVEISKEI 150

Query: 162 EAYL 164
           + +L
Sbjct: 151 DEHL 154

BLAST of Lag0021557 vs. ExPASy Swiss-Prot
Match: Q9SSK9 (MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.4e-23
Identity = 51/124 (41.13%), Postives = 79/124 (63.71%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           +P+ ++K SP N+   +L +G+WG VGSI++W Y   G+  V K ++EAV+ +KNLITF 
Sbjct: 46  KPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYVHDGEAKVAKERIEAVEPDKNLITFR 105

Query: 102 WIEGTLLEYYKKFQVTVQCVQK--HMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDI 161
            IEG L++ YK F +T+Q   K    GS+V W  E+E +  E+ +   L QF   +SK+I
Sbjct: 106 VIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLEYEKISEEVAHPETLLQFCVEVSKEI 165

Query: 162 EAYL 164
           + +L
Sbjct: 166 DEHL 169

BLAST of Lag0021557 vs. ExPASy Swiss-Prot
Match: Q941R6 (MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 4.5e-23
Identity = 52/124 (41.94%), Postives = 77/124 (62.10%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           RP+ ++K +P  +   EL +G+WGKVGSI++W Y   G+  V K ++EAV+ EKNLITF 
Sbjct: 44  RPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDGEAKVAKERIEAVEPEKNLITFR 103

Query: 102 WIEGTLLEYYKKFQVTVQCVQKH--MGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDI 161
            IEG LL+ YK F +T+Q   K    GSVV W  E+E +  ++ +      F   +SK+I
Sbjct: 104 VIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEKIDDKVAHPETFLDFCVEVSKEI 163

Query: 162 EAYL 164
           + +L
Sbjct: 164 DEHL 167

BLAST of Lag0021557 vs. ExPASy Swiss-Prot
Match: P85524 (Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.2e-21
Identity = 50/121 (41.32%), Postives = 72/121 (59.50%), Query Frame = 0

Query: 42  RPYLLTKISPENLHSSELLKGEWGKVGSIIYWTYTLGGKPSVVKHKVEAVDEEKNLITFT 101
           R YL++++SP N+   +LL+G WG VGS+I++ YT+ GK    K  VEA+DEE   +TF 
Sbjct: 27  RLYLISEMSPVNIQGVDLLEGNWGTVGSVIFFKYTIDGKEKTAKDIVEAIDEETKSVTFK 86

Query: 102 WIEGTLLEYYKKFQVTVQCVQKHMGSVVRWITEFEILRVEIPYLRNLPQFYGSISKDIEA 161
            +EG L+E YK F + VQ   K   + V W   +E L+ ++     L  F   I+KDIE 
Sbjct: 87  IVEGDLMELYKTFIIIVQVDTKGEHNSVTWTFHYEKLKEDVEEPNTLMNFCIEITKDIET 146

Query: 162 Y 163
           Y
Sbjct: 147 Y 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022956029.13.4e-2848.06MLP-like protein 43 [Cucurbita moschata][more]
XP_023526913.14.4e-2846.21MLP-like protein 34 [Cucurbita pepo subsp. pepo][more]
XP_022979964.14.4e-2848.06MLP-like protein 43 [Cucurbita maxima][more]
XP_022944436.17.6e-2846.51MLP-like protein 34 [Cucurbita moschata][more]
XP_023526175.19.9e-2847.29MLP-like protein 43 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SSK79.6e-2644.35MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1[more]
Q9SSK56.2e-2545.16MLP-like protein 43 OS=Arabidopsis thaliana OX=3702 GN=MLP43 PE=1 SV=1[more]
Q9SSK93.4e-2341.13MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1[more]
Q941R64.5e-2341.94MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2[more]
P855244.2e-2141.32Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000916Bet v I/Major latex proteinSMARTSM01037Bet_v_1_2coord: 37..166
e-value: 2.2E-24
score: 97.0
IPR000916Bet v I/Major latex proteinPFAMPF00407Bet_v_1coord: 44..164
e-value: 6.7E-32
score: 110.5
IPR023393START-like domain superfamilyGENE3D3.30.530.20coord: 37..164
e-value: 1.0E-36
score: 128.3
NoneNo IPR availablePANTHERPTHR31907:SF4MLP-LIKE PROTEIN 34-RELATEDcoord: 40..162
NoneNo IPR availablePANTHERPTHR31907MLP-LIKE PROTEIN 423coord: 40..162
NoneNo IPR availableCDDcd07816Bet_v1-likecoord: 42..138
e-value: 7.48116E-27
score: 97.6452
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 45..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021557.1Lag0021557.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response