Lag0021547 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021547
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr7: 9010347 .. 9012653 (+)
RNA-Seq ExpressionLag0021547
SyntenyLag0021547
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTTGGGTTCCAAATTTGCCCTCTTCCACCAATCCTTTTCTCATTTCAGGTTCGAGGAAATCATTGGGGCGATGAGGGACAAACTGACAGGGCCTGATTTTGAGCTTGTGGTGATTTTTTGGTGGTCTGTGTGGAGCCTACGGAATAATCTGTTTTGGGGTGGGCAGTCAGACGGTCGGGATCTCTGGGCATATTCGAGTGATTACCTCCATGCCTTCCATGTCGGTGGGGGACGTTGCGGGGTAAGGGACTCCTTATGGGCTCAGTCGGGGGAGCAAGAAGAGCGCGGTGTATGGAGACCGCCCCCTAATAGGGTGCTGAAACTTAATATTGATGCTTCAGTACGGCCGGATACAGGGGAAGCGAGGGGTGGTTGTGTGCTGCGAGGGGCTGAGGGGAGGTATTCATGGCAGCTTGTTTGAGCTTACAGAGGTGTTGGAGCGTGGATTTGGCTGAGGGTTGGGCTGTGTATAAAGGGATCCAACTTGCTCGACAGTTGGGGTTTGTGGATTTTGTGGTGGAGACTGACTCTCTAAGACTGGTCAAAATTTTGAATGGGGAGCTGCATGATGTGTCGGAAGTGGGGCTGCTGATGGATGACATTCGACGGATCCTCAGTCCTTGGGTCAACGGTAAGGTGTTGTTTATTCCACGTCAGGGGAATAGGGTGGCGCATGTTCTGGCCCGCCTGGCCTTTTCATATGTTGATCGTGTATGGCTTGAGGAGTGGCCTAGCGAGGTCTCGGATGTCCTGAGGGGTGATTTTGCGTCAGTTGCGTGAGAAGGGCTTCTTAATGCGATAATGGTGTTTCGAGCTAAGCGTTCAGTCTCTCCTGATCGTTGTACCAAAGCAGGGAAGGTTTTCTGTTTCCCTTTCACTTGTCGTTTATACATTGTCTTATGGTTGCATTGTAGATGTTCCTGGGAATAATGTATGATGTCGATTGGGGGTGGTAGCATGTATTTACTAGGGGTAGCTAGGCATTGAGCCTAGATGCATCTGCATGGCAGTTTGGTGTTTGCATGTTACTCTGTTTGGGTTTTCTCTCTGTTTAGTTGAATTGTGGTCCGTTATGTGGGTGCTGGGTCTGGTCTGAGTGGGGTTTGGTTTTGGCCTTAGAAGGGGGGTGAGGTGTTGGGCCTTTTGTGATAATTTCTGTATGTGTTGGTATGCTCTATTTTGCCTGGAATTGAGGCGATGACTGATAACATATTGTGAGCCTGGTTTTAAATTATGTCTTGATTATTCTTGCCTATCTGTTCACGTGGCCTCCCTTTTGGCTAGTCTTGCAGGTCTAGGCTTGCTTTATGGGTTTCCCCAAGTCTGTTGTCACCTCGATAGGATATGGGGTTTAGCCGGAGAGTCTATTTGCGTTTTCAGATTATGGTTGTTTGCATGAGGCATAAAATAAGGGGTAAAAAGAGCTGCCTGCAGGGTGATCAGTTAGGCCAACCATGATAATGCTCTGGTTAGGGGTCCCTTCTCTGCAGTGCGAAAGCTAGTGCCCTGCCTCTGAGTGGAAAGAAAATGGTGAGTATGGAATGTTTTCGGAAGATTGTTTTCTGAGAATGAAAGAAGATTGAAAATGATATGAAAATGAAATGAAAATGAAATGAATAAGTCTGACTCGTATGTCGGATTTGAGAGGGAAAGTGTGAGTGAGGACAGAGTGGGCCCAGGCTGCTGGGTAGCGTGCTAGCTTCCTAATTTGTTCTCCTTTAGCTCTTAGGTGAGACTTTTGTTCCAGTGTAGGGGGAAGATGGACGGTGGGTGCTTATAAATCTGGTCAGGGCTTCTAGGGCTGCTCTAGGTGAGGCTTGTCACACACTCACGTGAGAATTGTCTGGATAACTTTGGCTTACTTCATTCATGTGGTCAGGTATATTTCGCCTCTTCTGGGTAGGATTTAGCTTTTGCCATGCAAGGGCGTTATCTATGAGGGTTCCCGATTCCAGAGCCCTTCAGTGGAAAGTATACTGCTTAGGCCTACCTGGGGTGTCCATGTGGGGTGGTTGGTTGTTGTAGTAATGGTCCTATGCTGTTTGGTTTTCTGTTTATGCAGTCTGCTTCACTTTCTGGAATATTGTTATGCAGGTTGTTTATGCATGCTTTGTGATGAGGATCTTAGGTGTGTATGATGGCTTTCGCTAGAATGTCGGCTCCTATGGTTTCTTGTTGGGAAGCTCAAGGGGCTGGAGGTGTGTCGTGGCAGATGGGCCTGTGGATAGGATGGATCAAGATAATGCGTCATCCTGCCATATTGTGCCATCAAGATTCCAGGTGGATGGACCAGTGCCCATGA

mRNA sequence

ATGTGGTTGGGTTCCAAATTTGCCCTCTTCCACCAATCCTTTTCTCATTTCAGGTTCGAGGAAATCATTGGGGCGATGAGGGACAAACTGACAGGGCCTGATTTTGAGCTTGTGGTGATTTTTTGGTGGTCTGTGTGGAGCCTACGGAATAATCTGTTTTGGGGTGGGCAGTCAGACGGTCGGGATCTCTGGGCATATTCGAGTGATTACCTCCATGCCTTCCATGTCGGTGGGGGACGTTGCGGGGTAAGGGACTCCTTATGGGCTCAGTCGGGGGAGCAAGAAGAGCGCGGTGTATGGAGACCGCCCCCTAATAGGGTGCTGAAACTTAATATTGATGCTTCAAGGTGTTGGAGCGTGGATTTGGCTGAGGGTTGGGCTGTGTATAAAGGGATCCAACTTGCTCGACAGTTGGGGTTTGTGGATTTTGTGGTGGAGACTGACTCTCTAAGACTGGTCAAAATTTTGAATGGGGAGCTGCATGATGTGTCGGAAGTGGGGCTGCTGATGGATGACATTCGACGGATCCTCAGTCCTTGGGTCAACGGTAAGGTGTTGTTTATTCCACGTCAGGGGAATAGGGTGGCGCATGTTCTGGCCCGCCTGGCCTTTTCATATGTTGATCGTGTATGGCTTGAGGAGTGGCCTAGCGAGGTCTCGGATGTCCTGAGGGGTGATTTTGCGTCAGGAAGGTTTTCTGTTTCCCTTTCACTTGTCGTTTATACATTGTCTTATGGTTGCATTGTAGATGTTCCTGGGAATAATTTGAATTGTGGTCCGTTATGTGGGTGCTGGGTCTGGTCTGAGTGGGGTTTGGTTTTGGCCTTAGAAGGGGGTGCGAAAGCTAGTGCCCTGCCTCTGAGTGGAAAGAAAATGGGGGAAGATGGACGGTGGGTGCTTATAAATCTGGTCAGGGCTTCTAGGGCTGCTCTAGGTATATTTCGCCTCTTCTGGGTAGGATTTAGCTTTTGCCATGCAAGGGCGTTATCTATGAGGGTTCCCGATTCCAGAGCCCTTCAGTGGAAAGTGTGTATGATGGCTTTCGCTAGAATGTCGGCTCCTATGGTTTCTTGTTGGGAAGCTCAAGGGGCTGGAGGTGTGTCGTGGCAGATGGGCCTGTGGATAGGATGGATCAAGATAATGCGTCATCCTGCCATATTGTGCCATCAAGATTCCAGGTGGATGGACCAGTGCCCATGA

Coding sequence (CDS)

ATGTGGTTGGGTTCCAAATTTGCCCTCTTCCACCAATCCTTTTCTCATTTCAGGTTCGAGGAAATCATTGGGGCGATGAGGGACAAACTGACAGGGCCTGATTTTGAGCTTGTGGTGATTTTTTGGTGGTCTGTGTGGAGCCTACGGAATAATCTGTTTTGGGGTGGGCAGTCAGACGGTCGGGATCTCTGGGCATATTCGAGTGATTACCTCCATGCCTTCCATGTCGGTGGGGGACGTTGCGGGGTAAGGGACTCCTTATGGGCTCAGTCGGGGGAGCAAGAAGAGCGCGGTGTATGGAGACCGCCCCCTAATAGGGTGCTGAAACTTAATATTGATGCTTCAAGGTGTTGGAGCGTGGATTTGGCTGAGGGTTGGGCTGTGTATAAAGGGATCCAACTTGCTCGACAGTTGGGGTTTGTGGATTTTGTGGTGGAGACTGACTCTCTAAGACTGGTCAAAATTTTGAATGGGGAGCTGCATGATGTGTCGGAAGTGGGGCTGCTGATGGATGACATTCGACGGATCCTCAGTCCTTGGGTCAACGGTAAGGTGTTGTTTATTCCACGTCAGGGGAATAGGGTGGCGCATGTTCTGGCCCGCCTGGCCTTTTCATATGTTGATCGTGTATGGCTTGAGGAGTGGCCTAGCGAGGTCTCGGATGTCCTGAGGGGTGATTTTGCGTCAGGAAGGTTTTCTGTTTCCCTTTCACTTGTCGTTTATACATTGTCTTATGGTTGCATTGTAGATGTTCCTGGGAATAATTTGAATTGTGGTCCGTTATGTGGGTGCTGGGTCTGGTCTGAGTGGGGTTTGGTTTTGGCCTTAGAAGGGGGTGCGAAAGCTAGTGCCCTGCCTCTGAGTGGAAAGAAAATGGGGGAAGATGGACGGTGGGTGCTTATAAATCTGGTCAGGGCTTCTAGGGCTGCTCTAGGTATATTTCGCCTCTTCTGGGTAGGATTTAGCTTTTGCCATGCAAGGGCGTTATCTATGAGGGTTCCCGATTCCAGAGCCCTTCAGTGGAAAGTGTGTATGATGGCTTTCGCTAGAATGTCGGCTCCTATGGTTTCTTGTTGGGAAGCTCAAGGGGCTGGAGGTGTGTCGTGGCAGATGGGCCTGTGGATAGGATGGATCAAGATAATGCGTCATCCTGCCATATTGTGCCATCAAGATTCCAGGTGGATGGACCAGTGCCCATGA

Protein sequence

MWLGSKFALFHQSFSHFRFEEIIGAMRDKLTGPDFELVVIFWWSVWSLRNNLFWGGQSDGRDLWAYSSDYLHAFHVGGGRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDASRCWSVDLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRRILSPWVNGKVLFIPRQGNRVAHVLARLAFSYVDRVWLEEWPSEVSDVLRGDFASGRFSVSLSLVVYTLSYGCIVDVPGNNLNCGPLCGCWVWSEWGLVLALEGGAKASALPLSGKKMGEDGRWVLINLVRASRAALGIFRLFWVGFSFCHARALSMRVPDSRALQWKVCMMAFARMSAPMVSCWEAQGAGGVSWQMGLWIGWIKIMRHPAILCHQDSRWMDQCP
Homology
BLAST of Lag0021547 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 113.6 bits (283), Expect = 3.9e-21
Identity = 77/229 (33.62%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 38  VVIFWWSVWSLRN----NLFWGGQSDGRDLWAYSSDYLHAFHVGGGRCGVRDSLWAQSGE 97
           +V+  W++W+ RN    +   GG     DL ++S +YL  +     +   R S  A    
Sbjct: 44  IVVLLWAIWNARNQTPQHFSLGGSLS--DLVSWSENYLQVY-----QAAQRSSXSALVVC 103

Query: 98  QEER-GVWRPPPNRVLKLNIDA------------------------------SRCWSVDL 157
           +  R  +WRPP   +LK+N+DA                              +R   VD 
Sbjct: 104 RPPRVAIWRPPAAPLLKVNVDAAFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDW 163

Query: 158 AEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRRILSPWVN 217
            EG+AVY+GI LA + GF+ F +ETDSLR+  +L  +  D SEVG+L   I+  LS    
Sbjct: 164 VEGFAVYEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSSHAE 223

Query: 218 G-KVLFIPRQGNRVAHVLARLAFSYVD-RVWLEEWPSEVSDVLRGDFAS 230
                F  R GN  AH+LA+LA +    ++W+EEWP E+S VL  D  S
Sbjct: 224 RVSFSFTHRNGNAXAHLLAQLALTSPHLQIWVEEWPDEISSVLAVDCRS 265

BLAST of Lag0021547 vs. NCBI nr
Match: TXG71533.1 (hypothetical protein EZV62_000112 [Acer yangbiense])

HSP 1 Score: 101.3 bits (251), Expect = 2.0e-17
Identity = 62/231 (26.84%), Postives = 111/231 (48.05%), Query Frame = 0

Query: 7   FALFHQSFSHFRFEEIIGAMRDKLTGPDFELVVIFWWSVWSLRNNLFWGGQ-SDGRDLWA 66
           FA    S  +  F E + +   +L   +FEL+ I WW VW  RN            +++ 
Sbjct: 36  FAAGDASLDNVPFIEFVISCSSQLVLKEFELLCIIWWRVWHRRNKAVHNNNILPAAEIYD 95

Query: 67  YSSDYLHAFHVGGGRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDASRCWSV----- 126
           +  +++  +       G  +  + Q  E      W  PP   +K+N DA+  + +     
Sbjct: 96  WGCNFITDYR------GANERQYVQKVEVLRPPRWLAPPFGSVKINTDAALNFQLKVAGF 155

Query: 127 ----DLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRRI 186
                +AE  A+ +G++ A    F    +E+D+L +V+ +N +    SEVG++++DI  +
Sbjct: 156 GNKPPIAEALAILEGLRFAFSRNFSKVCLESDALCVVQAINNKSPPSSEVGVVLNDIFLV 215

Query: 187 LSPWVNGKVLFIPRQGNRVAHVLARLAFSYV-DRVWLEEWPSEVSDVLRGD 227
           L  +      F+PR GN+VAH LA+L  ++  + VWL + P  V +++ GD
Sbjct: 216 LDRFSEVSFAFVPRLGNKVAHGLAKLELNHEGESVWLNDCPLCVENLVLGD 260

BLAST of Lag0021547 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 100.9 bits (250), Expect = 2.6e-17
Identity = 68/238 (28.57%), Postives = 109/238 (45.80%), Query Frame = 0

Query: 19   FEEIIGAMRDKLTGPDFELVVIFWWSVWSLRNN-LFWGGQSDGRDLWAYSSDYLHAFHVG 78
            F   I  M  + +  + EL++++ W +WS RN  +F G +SD R L A +   L A+   
Sbjct: 1215 FFSAIQEMWSRSSTAEAELMIVYCWVIWSARNKFIFEGKKSDSRFLAAKADSVLKAYQ-- 1274

Query: 79   GGRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDASRCWS------------------ 138
              R     ++        ++  W+PP   VLKLN+DA+                      
Sbjct: 1275 --RVSKPGNVHGAKDRGIDQQKWKPPSQNVLKLNVDAAVSTKDQKVGLGAIVRDAEGKIL 1334

Query: 139  ------------VDLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVG 198
                        V LAE  A++ G+Q+A Q+     +VE+D   +V++LN      +E+ 
Sbjct: 1335 AVGIKQAQFRERVSLAEAEAIHWGLQVANQISSSSLIVESDCKEVVELLNNTKGSRTEIH 1394

Query: 199  LLMDDIRRILSPWVNGKVLFIPRQGNRVAHVLARLAF--SYVDRVWLEEWPSEVSDVL 224
             ++ D+RR    +   +  FIPR  N  AH LA+ A   S  D VW+  +P+EV +VL
Sbjct: 1395 WILSDVRRESKEFKQVQFSFIPRTCNTYAHALAKFALRNSSTD-VWVGTFPAEVQNVL 1447

BLAST of Lag0021547 vs. NCBI nr
Match: XP_023928118.1 (uncharacterized protein LOC112039474 [Quercus suber])

HSP 1 Score: 99.8 bits (247), Expect = 5.8e-17
Identity = 72/242 (29.75%), Postives = 113/242 (46.69%), Query Frame = 0

Query: 19  FEEIIGAMRDKLTGPDFELVVIFWWSVWSLRNNLFWGGQ-SDGRDLWAYSSDYLHAFHVG 78
           F+E++G    KL+  +FEL VI  W +W+ RN + +GG+  D + L  ++ ++L  FH  
Sbjct: 83  FQELLG----KLSINEFELFVIQSWIIWNQRNTVVFGGKLKDPKWLNKWAKEFLDEFHQA 142

Query: 79  GGRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDA-----SRCWSVD----------- 138
            G+  +  S    S       VWRPPP+   KLN DA       C  V            
Sbjct: 143 QGQLRIPVSCLGDS-------VWRPPPDSTFKLNFDAVVFSKLSCSGVGAMIRNENGEVM 202

Query: 139 --------------LAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVG 198
                         +AE  A  + ++ A + GF D VVE D+L ++K L     D+S +G
Sbjct: 203 ATMLARVPHVVDSVVAEVIACRRAMKFACKAGFTDLVVEGDNLSVMKSLTTSETDLSWLG 262

Query: 199 LLMDDIRRILSPWVNGKVLFIPRQGNRVAHVLARLAFS-YVDRVWLEEWPSEVSDVLRGD 229
            ++ DI+ +   +      ++ R  N VA+ LAR A   + D  W+E+ P  V + L  D
Sbjct: 263 HIIQDIKWLTRSFRRVSFSYVRRAANSVAYGLARYAKDIHEDMYWMEDNPPPVLEALYYD 313

BLAST of Lag0021547 vs. NCBI nr
Match: XP_023884925.1 (uncharacterized protein LOC111997106 [Quercus suber])

HSP 1 Score: 94.4 bits (233), Expect = 2.4e-15
Identity = 67/234 (28.63%), Postives = 105/234 (44.87%), Query Frame = 0

Query: 28  DKLTGPDFELVVIFWWSVWSLRNNLFWGGQ-SDGRDLWAYSSDYLHAFHVGGGRCGVRDS 87
           ++L+  + EL     W VW+ RN L  GGQ      L   + +Y+  F     R GV   
Sbjct: 6   ERLSQDELELFWTQAWIVWNQRNCLLHGGQLKVPSSLNKRAEEYISEFKEAQSRLGV--- 65

Query: 88  LWAQSGEQEERGVWRPPPNRVLKLNIDASRCWSV-------------------------- 147
              Q  +Q     W+PPP    K+N DA+    +                          
Sbjct: 66  ---QRAQQPAGDTWQPPPPGEFKMNFDAAMFSELGKTGYGAIIRNENGEVMAAMSASGPM 125

Query: 148 ----DLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRRI 207
               D AE  A  + I+ A   GF   +VE D++ +V+ ++  + ++S +G+++DDIR +
Sbjct: 126 VRTSDEAEFLACRRAIEFAVDAGFSRMIVEGDNINVVQAISSSVENISPIGIVVDDIRHL 185

Query: 208 LS--PWVNGKVLFIPRQGNRVAHVLARLAFSYVDR--VWLEEWPSEVSDVLRGD 227
           L    WV+  +  I R GN VAHVLA+ A + +D    WLE+ P   ++ L  D
Sbjct: 186 LRSLQWVS--ICCIRRGGNHVAHVLAQYARNCLDEDLYWLEDSPPPAAEALYRD 231

BLAST of Lag0021547 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-21
Identity = 77/229 (33.62%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 38  VVIFWWSVWSLRN----NLFWGGQSDGRDLWAYSSDYLHAFHVGGGRCGVRDSLWAQSGE 97
           +V+  W++W+ RN    +   GG     DL ++S +YL  +     +   R S  A    
Sbjct: 44  IVVLLWAIWNARNQTPQHFSLGGSLS--DLVSWSENYLQVY-----QAAQRSSXSALVVC 103

Query: 98  QEER-GVWRPPPNRVLKLNIDA------------------------------SRCWSVDL 157
           +  R  +WRPP   +LK+N+DA                              +R   VD 
Sbjct: 104 RPPRVAIWRPPAAPLLKVNVDAAFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDW 163

Query: 158 AEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRRILSPWVN 217
            EG+AVY+GI LA + GF+ F +ETDSLR+  +L  +  D SEVG+L   I+  LS    
Sbjct: 164 VEGFAVYEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSSHAE 223

Query: 218 G-KVLFIPRQGNRVAHVLARLAFSYVD-RVWLEEWPSEVSDVLRGDFAS 230
                F  R GN  AH+LA+LA +    ++W+EEWP E+S VL  D  S
Sbjct: 224 RVSFSFTHRNGNAXAHLLAQLALTSPHLQIWVEEWPDEISSVLAVDCRS 265

BLAST of Lag0021547 vs. ExPASy TrEMBL
Match: A0A5B7BI33 (Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_037968 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.3e-18
Identity = 68/249 (27.31%), Postives = 107/249 (42.97%), Query Frame = 0

Query: 10  FHQSFSHFRFEEIIGAMRDKLTGPDFELVVIFWWSVWSLRNNLFWGGQ-SDGRDLWAYSS 69
           F  +  H  F +    + +KL     EL  +  W VW  RN ++   Q  D   +   + 
Sbjct: 127 FPDALVHLDFRQFFEYICNKLDSSFIELWCVIIWIVWGHRNAIYHDRQGKDPLRVVFDAQ 186

Query: 70  DYLHAFHVGGGRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDAS------------- 129
            YL+ +H    R  +  S+ +++G      VW PPP  + KLN+D S             
Sbjct: 187 QYLNEYHEAQHRLHIPPSVSSRAGS----SVWSPPPEDLFKLNVDGSWVPGSFSGGIGGV 246

Query: 130 -----------------RCWSVDLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNG 189
                             C S D AE  A++ G+  A+++G VD ++E+D L LV  +  
Sbjct: 247 IRDSRGLVIAGFAKPLKWCGSADYAEACAMFFGVAFAKEIGIVDVLIESDCLSLVNSVGC 306

Query: 190 ELHDVSEVGLLMDDIRRILSPWVNGKVLFIPRQGNRVAHVLARLAFSYVDR-VWLEEWPS 227
              D S +G + DDIRR +    + +V  + R  N+ AH +A  A    +  +W+E  PS
Sbjct: 307 SSPDFSHIGHITDDIRRGMVGLRSFQVRHVRRSANQTAHEIASFARDVDEELLWIENLPS 366

BLAST of Lag0021547 vs. ExPASy TrEMBL
Match: A0A5C7IQ65 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000112 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 9.7e-18
Identity = 62/231 (26.84%), Postives = 111/231 (48.05%), Query Frame = 0

Query: 7   FALFHQSFSHFRFEEIIGAMRDKLTGPDFELVVIFWWSVWSLRNNLFWGGQ-SDGRDLWA 66
           FA    S  +  F E + +   +L   +FEL+ I WW VW  RN            +++ 
Sbjct: 36  FAAGDASLDNVPFIEFVISCSSQLVLKEFELLCIIWWRVWHRRNKAVHNNNILPAAEIYD 95

Query: 67  YSSDYLHAFHVGGGRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDASRCWSV----- 126
           +  +++  +       G  +  + Q  E      W  PP   +K+N DA+  + +     
Sbjct: 96  WGCNFITDYR------GANERQYVQKVEVLRPPRWLAPPFGSVKINTDAALNFQLKVAGF 155

Query: 127 ----DLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRRI 186
                +AE  A+ +G++ A    F    +E+D+L +V+ +N +    SEVG++++DI  +
Sbjct: 156 GNKPPIAEALAILEGLRFAFSRNFSKVCLESDALCVVQAINNKSPPSSEVGVVLNDIFLV 215

Query: 187 LSPWVNGKVLFIPRQGNRVAHVLARLAFSYV-DRVWLEEWPSEVSDVLRGD 227
           L  +      F+PR GN+VAH LA+L  ++  + VWL + P  V +++ GD
Sbjct: 216 LDRFSEVSFAFVPRLGNKVAHGLAKLELNHEGESVWLNDCPLCVENLVLGD 260

BLAST of Lag0021547 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-15
Identity = 66/233 (28.33%), Postives = 107/233 (45.92%), Query Frame = 0

Query: 28  DKLTGPDFELVVIFWWSVWSLRN-NLFWGGQSDGR-DLWAYSSDYLHAFHVGGGRCGVRD 87
           D++     E + +F W++W+ RN ++F  G      ++  + +DYL  +        +  
Sbjct: 2   DRIKWTGLEELSVFLWAIWNARNQSMFTNGHGFLLVNMVDWVADYLQVYQSAQEGPSLPL 61

Query: 88  SLWAQSGEQEERGVWRPPPNRVLKLNIDA-------------------------SRCW-- 147
            L  + G       W PP     K+N+DA                         + C+  
Sbjct: 62  LLDGRGGRV---SAWCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFIT 121

Query: 148 ---SVDLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGLLMDDIRR 207
               V LAE  A  +G+ LA + G + F +ETDS ++  +L  +  D SE+G+L   IR 
Sbjct: 122 HVSDVTLAECLAAQEGVCLAIEAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRH 181

Query: 208 ILSP-WVNGKVLFIPRQGNRVAHVLARLAF-SYVDRVWLEEWPSEVSDVLRGD 227
           I+S   + G   F+ R+GN  AH LAR+   S    VW+EEW S++S+V+  D
Sbjct: 182 IVSSLHIGGGFSFVNREGNSGAHTLARMGMVSESFHVWVEEWLSDLSEVIAAD 231

BLAST of Lag0021547 vs. ExPASy TrEMBL
Match: A0A2P6SAP1 (Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0328181 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.7e-15
Identity = 74/254 (29.13%), Postives = 112/254 (44.09%), Query Frame = 0

Query: 20   EEIIGAMRDKLTGPDFELVVIFWWSVWSLRNNLFWGGQ-SDGRDLWAYSSDYLHAFHVGG 79
            EE +  + D +T     L  ++ W +WS RNNL W G   +  +   ++S +L  +    
Sbjct: 980  EEWVKNVIDVITEHHRNLFFVYLWVIWSERNNLVWNGSVFNAYNAAQWASKFLGEYQ--- 1039

Query: 80   GRCGVRDSLWAQSGEQEERGVWRPPPNRVLKLNIDAS----------------------- 139
                V  +   +SG    R  W  PP   LK+NID S                       
Sbjct: 1040 ---QVHPTRQKKSG--RTRAKWENPPTGRLKINIDGSYRPESGDGGIGVVIRDDKGSCMA 1099

Query: 140  -------RCWSVDLAEGWAVYKGIQLARQLGFVDFVVETDSLRLVKILNGELHDVSEVGL 199
                      S    E  A   G+ LA    + +F++E+D   +V  LN  L D SEVG 
Sbjct: 1100 AMARYFPNVLSAFHMEAEACRAGLLLAIYQEWDEFILESDCSLMVAALNQNLEDRSEVGR 1159

Query: 200  LMDDIRRILSPWVNGKVLFIPRQGNRVAHVLARLAFS-YVDRVWLEEWPSEVSDVLRGDF 239
            ++DD +  L+   + K+  + R+ N VA+ LA LA S +++ VWLEE P  + DVL  D 
Sbjct: 1160 IIDDCKSYLTSLHSIKISHVFREANGVANRLAHLARSKFLNDVWLEEAPVIIQDVLYEDL 1219

BLAST of Lag0021547 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-10
Identity = 53/206 (25.73%), Postives = 96/206 (46.60%), Query Frame = 0

Query: 36  ELVVIFWWSVWSLRNNL-FWGGQSDGRDLWAYSSDYLHAFHV--GGGRCGVRDSLWAQSG 95
           +LV    W +W  RN L F G + + +++   + D L  + +      CG +  +     
Sbjct: 358 QLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQV----- 417

Query: 96  EQEERGVWRPPPNRVLKLNIDAS------RC---WSVDLAEGWAVYKGIQLARQLGFV-- 155
            +   G WRPPP++ +K N DA+      RC   W +   +G   + G +   +L  V  
Sbjct: 418 NRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLE 477

Query: 156 ------------------DFVV-ETDSLRLVKILNGELHDVSEVGLLMDDIRRILSPWVN 209
                             ++V+ E+DS  L++ILN +      +   + D++R+LS +  
Sbjct: 478 AELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQRLLSQFTE 537

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150944.13.9e-2133.62uncharacterized protein LOC111018973 [Momordica charantia][more]
TXG71533.12.0e-1726.84hypothetical protein EZV62_000112 [Acer yangbiense][more]
XP_006491472.12.6e-1728.57uncharacterized protein LOC102626455 [Citrus sinensis][more]
XP_023928118.15.8e-1729.75uncharacterized protein LOC112039474 [Quercus suber][more]
XP_023884925.12.4e-1528.63uncharacterized protein LOC111997106 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DBJ71.9e-2133.62uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A5B7BI334.3e-1827.31Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_037968... [more]
A0A5C7IQ659.7e-1826.84RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000112 ... [more]
A0A6J1C4671.2e-1528.33uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A2P6SAP17.7e-1529.13Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1... [more]
Match NameE-valueIdentityDescription
AT4G29090.11.1e-1025.73Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 94..207
e-value: 1.2E-7
score: 33.7
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 112..203
e-value: 4.0E-17
score: 62.2
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 104..205
score: 9.249923
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 119..227
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 119..227
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 98..201
e-value: 2.05518E-15
score: 70.0356
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 113..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021547.1Lag0021547.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity