Lag0021546 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0021546
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Protein RALF-like 32
Location	chr7: 9000690 .. 9001025 (-)
RNA-Seq Expression	Lag0021546
Synteny	Lag0021546

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTTACAACCTTGCTTTCTTCTCCTCCTCCTTCTCTTCCTCCTCCAAGATCTTCGTCTCCTTCGAGCTTCGTCGATGAGCTCTTGCAACGGCTCCATAGCCGAGTGTGATAGCGAGAAGGAGATGCTGATGGAGTCGGAGATAAGTCGAAGGTTTCTCGAACAACAGAAGAAGAAATACATCTCCATTGGAGCTTTGAAGAAAGATCGACCGGCTTGTGATGGCAGCGGTGGCGGTCAACCTTACACCAAAAGCGGAAGCTGCTCTCCGCCCCCGACTAATCCTTACAACCGAGGCTGCTCGAAGATATATCGTTGTAGGTCCGACGATTGA

mRNA sequence

ATGTTTTTACAACCTTGCTTTCTTCTCCTCCTCCTTCTCTTCCTCCTCCAAGATCTTCGTCTCCTTCGAGCTTCGTCGATGAGCTCTTGCAACGGCTCCATAGCCGAGTGTGATAGCGAGAAGGAGATGCTGATGGAGTCGGAGATAAGTCGAAGGTTTCTCGAACAACAGAAGAAGAAATACATCTCCATTGGAGCTTTGAAGAAAGATCGACCGGCTTGTGATGGCAGCGGTGGCGGTCAACCTTACACCAAAAGCGGAAGCTGCTCTCCGCCCCCGACTAATCCTTACAACCGAGGCTGCTCGAAGATATATCGTTGTAGGTCCGACGATTGA

Coding sequence (CDS)

ATGTTTTTACAACCTTGCTTTCTTCTCCTCCTCCTTCTCTTCCTCCTCCAAGATCTTCGTCTCCTTCGAGCTTCGTCGATGAGCTCTTGCAACGGCTCCATAGCCGAGTGTGATAGCGAGAAGGAGATGCTGATGGAGTCGGAGATAAGTCGAAGGTTTCTCGAACAACAGAAGAAGAAATACATCTCCATTGGAGCTTTGAAGAAAGATCGACCGGCTTGTGATGGCAGCGGTGGCGGTCAACCTTACACCAAAAGCGGAAGCTGCTCTCCGCCCCCGACTAATCCTTACAACCGAGGCTGCTCGAAGATATATCGTTGTAGGTCCGACGATTGA

Protein sequence

MFLQPCFLLLLLLFLLQDLRLLRASSMSSCNGSIAECDSEKEMLMESEISRRFLEQQKKKYISIGALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSDD

Homology

[Close]

BLAST of Lag0021546 vs. NCBI nr
Match: XP_038900965.1 (protein RALF-like 32 [Benincasa hispida])

HSP 1 Score: 173.7 bits (439), Expect = 8.8e-40
Identity = 94/110 (85.45%), Postives = 96/110 (87.27%), Query Frame = 0


Query: 3   LQPCFLLL-LLLFLLQDLRLLRASSMSSCNGSIAECDSEKEMLMESEISRRFLEQQKKKY 62
           LQ CFLLL L L LLQDL L+RA S  SCNGSIAEC SE+EMLMESEISRRFLEQ  KKY
Sbjct: 25  LQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEISRRFLEQH-KKY 84

Query: 63  ISIGALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSDD 112
           ISIGALKKD PACDGSGGGQPYTKSGSC PPPTNPY RGCSKIYRCRSDD
Sbjct: 85  ISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSDD 133

[Close]

BLAST of Lag0021546 vs. NCBI nr
Match: KAA0067637.1 (protein RALF-like 32 [Cucumis melo var. makuwa] >TYK23639.1 protein RALF-like 32 [Cucumis melo var. makuwa])

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-39
Identity = 94/115 (81.74%), Postives = 99/115 (86.09%), Query Frame = 0


Query: 1   MFLQPCFLLLLL----LFLLQDLRLLRASSMSSCNGSIAECDSEKEMLMESEISRRFLEQ 60
           M LQPCFLLLLL    LFLLQDL L+RASS  SCNGSIAEC SE EMLMESEISRRFL Q
Sbjct: 10  MSLQPCFLLLLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEMLMESEISRRFLAQ 69

Query: 61  QKKKYISIGALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSDD 112
           Q KKYISIGALKKD PACDG+ GG+PYTKSGSC+PPP NPY+RGC KIYRCRSDD
Sbjct: 70  Q-KKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD 123

[Close]

BLAST of Lag0021546 vs. NCBI nr
Match: XP_022138047.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 172.6 bits (436), Expect = 2.0e-39
Identity = 93/108 (86.11%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 4   QPCFLLLLLLFLLQDLRLLRASSMSSCNGSIAECDSEKEMLMESEISRRFLEQQKKKYIS 63
           QP F  LLLLFLLQ L LLRAS  S CNGSIAEC++EKEMLMESEISRRFLEQQ KKYIS
Sbjct: 13  QPFF--LLLLFLLQALCLLRASFASPCNGSIAECNTEKEMLMESEISRRFLEQQ-KKYIS 72

Query: 64  IGALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSDD 112
           IGALKKDRPACDGSG GQPYTKSGSC PPP+NPY+RGCSKIYRCRSDD
Sbjct: 73  IGALKKDRPACDGSGRGQPYTKSGSCVPPPSNPYDRGCSKIYRCRSDD 117

[Close]

BLAST of Lag0021546 vs. NCBI nr
Match: XP_004147646.3 (protein RALF-like 32 [Cucumis sativus])

HSP 1 Score: 172.6 bits (436), Expect = 2.0e-39
Identity = 92/113 (81.42%), Postives = 99/113 (87.61%), Query Frame = 0


Query: 1   MFLQPCFLLL--LLLFLLQDLRLLRASSMSSCNGSIAECDSEKEMLMESEISRRFLEQQK 60
           M LQ CFLLL  L LFL QDL L+RASS  SCNGSIAEC +E+EMLMESEI+RRFLEQQ 
Sbjct: 17  MSLQTCFLLLLFLFLFLFQDLCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQ- 76

Query: 61  KKYISIGALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSDD 112
           KKYISIGALKKD PACDG+ GGQPYT+SGSC+PPP NPYNRGCSKIYRCRSDD
Sbjct: 77  KKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of Lag0021546 vs. NCBI nr
Match: XP_023528073.1 (protein RALF-like 32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 167.5 bits (423), Expect = 6.3e-38
Identity = 88/106 (83.02%), Postives = 93/106 (87.74%), Query Frame = 0


Query: 6   CFLLLLLLFLLQDLRLLRASSMSSCNGSIAECDSEKEMLMESEISRRFLEQQKKKYISIG 65
           CFLL LL  L+QDL L+RASS  SCNGSIAEC  E+E LMESEISRRFLEQQ KKYISIG
Sbjct: 9   CFLLFLL--LVQDLCLVRASSKHSCNGSIAECGGEEETLMESEISRRFLEQQ-KKYISIG 68

Query: 66  ALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSDD 112
           ALKKD PACDG GGGQPYT+SGSC+PPP NPYNRGCSKIYRCRSDD
Sbjct: 69  ALKKDHPACDGGGGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 111

[Close]

BLAST of Lag0021546 vs. ExPASy Swiss-Prot
Match: O23262 (Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 7.1e-16
Identity = 54/109 (49.54%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 7   FLLLLLLFLLQDLRLLRASSMSSCNGSIAECDS-----EKEMLMESEISRRFLEQQKKKY 66
           FL LLL  +       +ASS S CNGS+AEC S     E  ++MES  S+R  E+Q  K 
Sbjct: 16  FLCLLLAHVTS-----KASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHK- 75

Query: 67  ISIGALKKDRPACDGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCRSD 111
           +S GAL++++PACDG   G+ Y  S  C PPP+NPY+RGCSK YRC  D
Sbjct: 76  LSYGALRRNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of Lag0021546 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 9.9e-10
Identity = 42/94 (44.68%), Postives = 54/94 (57.45%), Query Frame = 0


Query: 16  LQDLRLLRASSMSSCNGSIAEC-DSEKEMLMESEISRRFLEQQKKKYISIGALKKDRPAC 75
           L  +R   +S  S C GSIAEC   E+EM  +S+ISRR L Q  KKYIS GA++++   C
Sbjct: 31  LDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILAQ--KKYISYGAMRRNSVPC 90

Query: 76  DGSGGGQPYTKSGSCSPPPTNPYNRGCSKIYRCR 109
              G      + G+      NPY+RGCS I RCR
Sbjct: 91  SRRGASYYNCQRGA----QANPYSRGCSTITRCR 118

[Close]

BLAST of Lag0021546 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.2e-09
Identity = 38/82 (46.34%), Postives = 49/82 (59.76%), Query Frame = 0


Query: 28  SSCNGSIAECDSEKEMLMESEISRRFLEQQKKKYISIGALKKDRPACDGSGGGQPYTKSG 87
           S C+GSIAEC   +E  M+SEI+RR L     KYIS  +LK++   C   G      ++G
Sbjct: 45  SGCHGSIAECIGAEEEEMDSEINRRIL--ATTKYISYQSLKRNSVPCSRRGASYYNCQNG 104

Query: 88  SCSPPPTNPYNRGCSKIYRCRS 110
           +      NPY+RGCSKI RCRS
Sbjct: 105 A----QANPYSRGCSKIARCRS 120

[Close]

BLAST of Lag0021546 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.4e-09
Identity = 40/90 (44.44%), Postives = 51/90 (56.67%), Query Frame = 0


Query: 21  LLRASSMSSCNGSIAECDSEKEML-MESEISRRFLEQQKKKYISIGALKKDRPACDGSGG 80
           ++ A S   C GSI EC +E+E   ++SE +RR L    KKYIS GAL+K+   C   G 
Sbjct: 32  VMPARSGGGCKGSIGECIAEEEEFELDSESNRRIL--ATKKYISYGALQKNSVPCSRRGA 91

Query: 81  GQPYTKSGSCSPPPTNPYNRGCSKIYRCRS 110
                K G+      NPY+RGCS I RCRS
Sbjct: 92  SYYNCKPGA----QANPYSRGCSAITRCRS 115

The following BLAST results are available for this feature:

BLAST of Lag0021546 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900965.1	8.8e-40	85.45	protein RALF-like 32 [Benincasa hispida]	[more]
KAA0067637.1	1.2e-39	81.74	protein RALF-like 32 [Cucumis melo var. makuwa] >TYK23639.1 protein RALF-like 32...	[more]
XP_022138047.1	2.0e-39	86.11	protein RALF-like 32 [Momordica charantia]	[more]
XP_004147646.3	2.0e-39	81.42	protein RALF-like 32 [Cucumis sativus]	[more]
XP_023528073.1	6.3e-38	83.02	protein RALF-like 32 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0021546 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23262	7.1e-16	49.54	Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1	[more]
Q9MA62	9.9e-10	44.68	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
Q9SRY3	2.2e-09	46.34	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
Q945T0	8.4e-09	44.44	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 42..108 e-value: 7.4E-24 score: 84.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 81..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 69..99
None	No IPR available	PANTHER	PTHR33136:SF4	PROTEIN RALF-LIKE 32	coord: 4..108
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 4..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0021546.1	Lag0021546.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region