Lag0021531 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0021531 vs. NCBI nr
Match: XP_022949236.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita moschata] >KAG7029039.1 Diacylglycerol O-acyltransferase 3, cytosolic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.2 bits (1341), Expect = 6.8e-144
Identity = 280/342 (81.87%), Postives = 295/342 (86.26%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGG V   TRD GL  RRN+
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NTSYR RKA    LSQPF DQWQT AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSL 300
           AR +SVGR+EVCMGNKCKKAGAAALMEEFERV+GAD VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

Query: 301 DIEARSANPLCIGVGVEDVGRIVADYLGQEGQ-KQSRFAPAI 338
           D+E RSANPLCIGVGVEDVGRIV D+LG +G+ KQ RFAP+I
Sbjct: 301 DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPSI 342

BLAST of Lag0021531 vs. NCBI nr
Match: KAG6597595.1 (Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520.0 bits (1338), Expect = 1.5e-143
Identity = 279/342 (81.58%), Postives = 295/342 (86.26%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGG V   TRD GL  RRN+
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    +SQPF DQWQT AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKANSPSISQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSL 300
           AR +SVGR+EVCMGNKCKKAGAAALMEEFERV+GAD VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

Query: 301 DIEARSANPLCIGVGVEDVGRIVADYLGQEGQ-KQSRFAPAI 338
           D+E RSANPLCIGVGVEDVGRIVAD+LG +G+ KQ RFAP+I
Sbjct: 301 DMETRSANPLCIGVGVEDVGRIVADHLGGQGEHKQPRFAPSI 342

BLAST of Lag0021531 vs. NCBI nr
Match: XP_023539958.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516.5 bits (1329), Expect = 1.7e-142
Identity = 278/342 (81.29%), Postives = 293/342 (85.67%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGG V   TRD GL  RRN+
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    LSQPF DQWQ  AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKANSPSLSQPFSDQWQANAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSL 300
           AR ASVGR+EVCMGNKCKKAGAAALMEEFERV+GAD VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

Query: 301 DIEARSANPLCIGVGVEDVGRIVADYLGQEGQ-KQSRFAPAI 338
           D+E RS NPLCIGVGVEDVGRIVA++LG +G+ KQ RFAP+I
Sbjct: 301 DMETRSPNPLCIGVGVEDVGRIVAEHLGGQGEHKQPRFAPSI 342

BLAST of Lag0021531 vs. NCBI nr
Match: XP_022972166.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita maxima])

HSP 1 Score: 505.0 bits (1299), Expect = 5.1e-139
Identity = 276/342 (80.70%), Postives = 289/342 (84.50%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSRRLPGL RREIAQSS+EVSG DARRAPCSLS+SGG VG  TRD GL  RRN+
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSVEVSGHDARRAPCSLSISGGSVGTETRDFGLHVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGL G  SAA   GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLPGDSSAALAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR  T ++SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLPTAYDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    LSQ F DQWQ  AIQGSAL PPLQT QL SN  N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKASSPTLSQLFSDQWQANAIQGSALAPPLQTHQLNSNIGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSL 300
           AR ASVGR+EVCMGNKCKKAGAAALMEEF RV+GAD VVCGCKCMG+CRDGPNVRVSGSL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFGRVMGADSVVCGCKCMGRCRDGPNVRVSGSL 300

Query: 301 DIEARSANPLCIGVGVEDVGRIVADYLGQEG-QKQSRFAPAI 338
           D+E  SANPLCIGVGVEDVGRIVAD LG +G QKQSRFAP+I
Sbjct: 301 DMETGSANPLCIGVGVEDVGRIVADRLGGQGEQKQSRFAPSI 342

BLAST of Lag0021531 vs. NCBI nr
Match: XP_038900889.1 (diacylglycerol O-acyltransferase 3 [Benincasa hispida])

HSP 1 Score: 488.8 bits (1257), Expect = 3.8e-134
Identity = 266/337 (78.93%), Postives = 287/337 (85.16%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSR L   FR +  Q S+E+S ADARRA CSLS+SGG VG GTR LGL  R N+
Sbjct: 1   MEVSGAVSRHLSAFFRHDFDQ-SIELSRADARRASCSLSISGGSVGTGTRTLGLASRWNM 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRFCDDGHLRYYVGP CHGGRLKKEKEAVKKKLKLLKGLS   +  YES  +F  D G
Sbjct: 61  DSSRFCDDGHLRYYVGPTCHGGRLKKEKEAVKKKLKLLKGLS---ATDYESSLLFHLDHG 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           S+GEFQ DRFSIEETREAL RQLQQLK+EEKEQKRK+KLEKAKLKAARAQTIH+SS SSS
Sbjct: 121 SIGEFQADRFSIEETREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARAQTIHDSSFSSS 180

Query: 181 ESSDNESNTINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARPA 240
           ESSDNE++ IN SYRIRKALSQPFPDQ+QT AIQGS LPPPLQT  L S + N GV+   
Sbjct: 181 ESSDNEAHMINKSYRIRKALSQPFPDQYQTNAIQGSTLPPPLQTQLLNSKSGNVGVS--T 240

Query: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSLDIEA 300
           SVGRIEVCMGNKCKKAGAAALMEEF RV+GADG VCGCKCMGKCRDGPNVRVSG+LDI+ 
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFGRVMGADGAVCGCKCMGKCRDGPNVRVSGALDIQ- 300

Query: 301 RSANPLCIGVGVEDVGRIVADYLGQE-GQKQSRFAPA 337
              NPLCIGVGVEDVGRIVA+YLGQE GQ+QSRFAPA
Sbjct: 301 ---NPLCIGVGVEDVGRIVAEYLGQERGQQQSRFAPA 327

BLAST of Lag0021531 vs. ExPASy Swiss-Prot
Match: Q9C5W0 (Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 4.0e-38
Identity = 140/360 (38.89%), Postives = 190/360 (52.78%), Query Frame = 0

Query: 24  MEVSGADARRAPC--SLSVSG-----GFVGVGTRDLGLVGRR---NVDSSRFCDDGHLRY 83
           MEVSG   R+ PC  S SV+G      F G  TR +G   RR    V ++ F D GH+ Y
Sbjct: 1   MEVSGVVLRQIPCVSSGSVAGLRLVSEFSG-NTRTVGFRTRRFRGIVCNNEFADKGHVNY 60

Query: 84  YVGP-RCHGGR-----LKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDGSVGEFQG 143
           Y+ P RC   +     ++KEK+A+KKK K+LK LS  L      GF    + G VGE Q 
Sbjct: 61  YIEPTRCGEEKEKVKVMEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEIQT 120

Query: 144 DRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHES-SSSSSESSDNE 203
              +I E  E L +QL+QLKAEEK  K++RK EKAK KA +  T  +S SSSSSESSD++
Sbjct: 121 K--TISEATEILVKQLEQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSD 180

Query: 204 SN-------------------------TINTSYRIRK-ALSQPFPDQWQTKAIQGSALPP 263
            +                         T+ T  RI++ A+S     +    A+Q S + P
Sbjct: 181 CDKGKVVDMSSLRNKAKPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTSTIFP 240

Query: 264 ----PLQTLQLGSNTENTGVARPASVGRIEVCMGNKCKKAGAAALMEEFER-VVGADGVV 323
               P QTL+        G+     + R+EVCMG KCK++G A L++EF+R + G +G  
Sbjct: 241 SMANPGQTLKTVEAVSVVGL----PLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSA 300

Query: 324 CGCKCMGKCRDGPNVRVSGSLDIEARS-----ANPLCIGVGVEDVGRIVADYLGQEGQKQ 331
             CKCMGKCRDGPNVRV    D          +  LC+GVG++DV  IV  +  +E  ++
Sbjct: 301 VACKCMGKCRDGPNVRVVKETDAVMTDSVRTPSKTLCVGVGLQDVETIVTSFFDEECSRE 353

BLAST of Lag0021531 vs. ExPASy TrEMBL
Match: A0A6J1GBG4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111452646 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.3e-144
Identity = 280/342 (81.87%), Postives = 295/342 (86.26%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSRRLPGL RREIAQSSMEVSG DARRAPCSLS+SGG V   TRD GL  RRN+
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGLSG  SAA+  GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR QT H+SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NTSYR RKA    LSQPF DQWQT AIQGSAL PPLQT QL SNT N GV
Sbjct: 181 ESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSL 300
           AR +SVGR+EVCMGNKCKKAGAAALMEEFERV+GAD VVCGCKCMG+CRDGPNVRVS SL
Sbjct: 241 ARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSSL 300

Query: 301 DIEARSANPLCIGVGVEDVGRIVADYLGQEGQ-KQSRFAPAI 338
           D+E RSANPLCIGVGVEDVGRIV D+LG +G+ KQ RFAP+I
Sbjct: 301 DMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPRFAPSI 342

BLAST of Lag0021531 vs. ExPASy TrEMBL
Match: A0A6J1I582 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111470783 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.5e-139
Identity = 276/342 (80.70%), Postives = 289/342 (84.50%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAVSRRLPGL RREIAQSS+EVSG DARRAPCSLS+SGG VG  TRD GL  RRN+
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSVEVSGHDARRAPCSLSISGGSVGTETRDFGLHVRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           +S RF DDGH+RYYVG RCHGGRLKKEKEAVKKKLKLLKGL G  SAA   GFVFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLPGDSSAALAFGFVFRFDDS 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           SVGEFQ DRFSIEETRE L RQLQQLKAEEKEQKRKRKLEKAKLKAAR  T ++SSSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLPTAYDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKA----LSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGV 240
           ESS  E N  NT YR RKA    LSQ F DQWQ  AIQGSAL PPLQT QL SN  N GV
Sbjct: 181 ESSAIEGNMTNTRYRTRKASSPTLSQLFSDQWQANAIQGSALAPPLQTHQLNSNIGNFGV 240

Query: 241 ARPASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSL 300
           AR ASVGR+EVCMGNKCKKAGAAALMEEF RV+GAD VVCGCKCMG+CRDGPNVRVSGSL
Sbjct: 241 ARSASVGRVEVCMGNKCKKAGAAALMEEFGRVMGADSVVCGCKCMGRCRDGPNVRVSGSL 300

Query: 301 DIEARSANPLCIGVGVEDVGRIVADYLGQEG-QKQSRFAPAI 338
           D+E  SANPLCIGVGVEDVGRIVAD LG +G QKQSRFAP+I
Sbjct: 301 DMETGSANPLCIGVGVEDVGRIVADRLGGQGEQKQSRFAPSI 342

BLAST of Lag0021531 vs. ExPASy TrEMBL
Match: A0A6J1GV38 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111457454 PE=4 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.6e-133
Identity = 260/334 (77.84%), Postives = 289/334 (86.53%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAV+RRL  LFR EIAQ S++V+ ADARRAPCSLSVSGG V  GT  +GLVGRRN+
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQ-SVDVASADARRAPCSLSVSGGSVSTGTHVIGLVGRRNI 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRF D+GHLRYYVGP C G RLKKEKEAVKKKLKLLKGLSG  S  +ESG VFRFDD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDC 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHE-SSSSS 180
            +G+FQ DRFSIEETREAL +QLQQLK+EEK+QKR+RKLEKAKLKA R +TIHE SSSSS
Sbjct: 121 LIGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRVKTIHESSSSSS 180

Query: 181 SESSDNESNTINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARP 240
           SESSDNE    NTSY+IRKALSQPFPDQW+T  I+ S LPPPLQ+  L S+T+N GVAR 
Sbjct: 181 SESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARS 240

Query: 241 ASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSLDIE 300
            SVGR+EVCMGNKCKKAGAAALMEEFERV+GADG VCGCKCMGKCRDGPNVRVSG+L  +
Sbjct: 241 VSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQ 300

Query: 301 ARSANPLCIGVGVEDVGRIVADYLGQEGQ-KQSR 333
           + SANPLCIGVGVEDVGRIVAD+LGQ+G+ KQSR
Sbjct: 301 SCSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 333

BLAST of Lag0021531 vs. ExPASy TrEMBL
Match: A0A6J1IRZ4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111479426 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 7.1e-131
Identity = 259/334 (77.54%), Postives = 285/334 (85.33%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEVSGAV+RRL  LFR EI Q S++V+ ADARRAPCSLSVSGG V  GTR +GLVGRRNV
Sbjct: 1   MEVSGAVTRRLSSLFRCEIVQ-SVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNV 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRF D+GHLRYYVGP C G RLKKEKEAVKKK KLLKGLSG  S  +ESG VFR DD 
Sbjct: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKFKLLKGLSGYSSTDHESGLVFRLDDC 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHE-SSSSS 180
            +G+ Q DRFSIEETREAL RQLQQLK+EEK+QKRKRKLEKAKLKA RA+TIH+ SSSSS
Sbjct: 121 LIGDSQADRFSIEETREALIRQLQQLKSEEKDQKRKRKLEKAKLKATRAKTIHKSSSSSS 180

Query: 181 SESSDNESNTINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARP 240
           SESSDNE   INT YRIRKALSQPFPDQW+T  I+ S LPPPLQ+  L S+T+N GVA+ 
Sbjct: 181 SESSDNEGYMINTRYRIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVAQS 240

Query: 241 ASVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSLDIE 300
            SVGR+EVCMGNKCKKAGAAALMEEFERV+GADG VCGCKCMGKCRDGPNVRVSG+L  +
Sbjct: 241 VSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALARQ 300

Query: 301 ARSANPLCIGVGVEDVGRIVADYLGQE-GQKQSR 333
           + SANPLCIGVGV DVGRIVAD+LGQ+ G KQSR
Sbjct: 301 SCSANPLCIGVGVGDVGRIVADHLGQQGGLKQSR 333

BLAST of Lag0021531 vs. ExPASy TrEMBL
Match: E5GC43 (Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.7e-125
Identity = 249/337 (73.89%), Postives = 277/337 (82.20%), Query Frame = 0

Query: 1   MEVSGAVSRRLPGLFRREIAQSSMEVSGADARRAPCSLSVSGGFVGVGTRDLGLVGRRNV 60
           MEV GAVSR+LPGLFRR+ +Q S+EV  AD RR PCSLS+SG  VG  TR LGLV RR +
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQ-SVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRM 60

Query: 61  DSSRFCDDGHLRYYVGPRCHGGRLKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDG 120
           DSSRFCDDG+LRYYVGP C GG LKKEKEAVKKKLKLLKGLS   +  YES  +FRFD G
Sbjct: 61  DSSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLS---ATDYESSLLFRFDHG 120

Query: 121 SVGEFQGDRFSIEETREALGRQLQQLKAEEKEQKRKRKLEKAKLKAARAQTIHESSSSSS 180
           S+ EFQ DRFSIE+TREAL RQLQQLK EEKEQKRK+KLEKAKLKAAR +TIH+SSSSSS
Sbjct: 121 SIDEFQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSS 180

Query: 181 ESSDNESNTINTSYRIRKALSQPFPDQWQTKAIQGSALPPPLQTLQLGSNTENTGVARPA 240
           ESSDNE +    SY+++KALSQPFPDQ++T AI  S LP PLQT  L S TEN GV R  
Sbjct: 181 ESSDNEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRST 240

Query: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVVGADGVVCGCKCMGKCRDGPNVRVSGSLDIEA 300
           SVGRIEVCMGNKCKKAGAAALMEEFERV+G +  VCGCKCMGKCRDGPNVRV GS++++ 
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ- 300

Query: 301 RSANPLCIGVGVEDVGRIVADYLGQE-GQKQSRFAPA 337
              NPLCIGVGVEDV RIVA+YLGQ+ GQ QSR APA
Sbjct: 301 ---NPLCIGVGVEDVDRIVAEYLGQKGGQTQSRLAPA 329

BLAST of Lag0021531 vs. TAIR 10
Match: AT1G48300.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 96 Blast hits to 87 proteins in 37 species: Archae - 0; Bacteria - 2; Metazoa - 12; Fungi - 2; Plants - 65; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 136.7 bits (343), Expect = 3.4e-32
Identity = 111/284 (39.08%), Postives = 153/284 (53.87%), Query Frame = 0

Query: 84  LKKEKEAVKKKLKLLKGLSGGLSAAYESGFVFRFDDGSVGEFQGDRFSIEETREALGRQL 143
           ++KEK+A+KKK K+LK LS  L      GF    + G VGE Q    +I E  E L +QL
Sbjct: 1   MEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEIQTK--TISEATEILVKQL 60

Query: 144 QQLKAEEKEQKRKRKLEKAKLKAARAQTIHES-SSSSSESSDNESN-------------- 203
           +QLKAEEK  K++RK EKAK KA +  T  +S SSSSSESSD++ +              
Sbjct: 61  EQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSDCDKGKVVDMSSLRNKA 120

Query: 204 -----------TINTSYRIRK-ALSQPFPDQWQTKAIQGSALPP----PLQTLQLGSNTE 263
                      T+ T  RI++ A+S     +    A+Q S + P    P QTL+      
Sbjct: 121 KPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTSTIFPSMANPGQTLKTVEAVS 180

Query: 264 NTGVARPASVGRIEVCMGNKCKKAGAAALMEEFER-VVGADGVVCGCKCMGKCRDGPNVR 323
             G+     + R+EVCMG KCK++G A L++EF+R + G +G    CKCMGKCRDGPNVR
Sbjct: 181 VVGL----PLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSAVACKCMGKCRDGPNVR 240

Query: 324 VSGSLDIEARS-----ANPLCIGVGVEDVGRIVADYLGQEGQKQ 331
           V    D          +  LC+GVG++DV  IV  +  +E  ++
Sbjct: 241 VVKETDAVMTDSVRTPSKTLCVGVGLQDVETIVTSFFDEECSRE 278