Lag0021471 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0021471 vs. NCBI nr
Match: XP_038880915.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 693.0 bits (1787), Expect = 1.7e-195
Identity = 344/433 (79.45%), Postives = 392/433 (90.53%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           M+SS+  SHSIDI A +QGSS++ESLLSS+EGKLEA CSSITIF+ P++ISIED++ FVP
Sbjct: 1   MESSKPFSHSIDISAIAQGSSQEESLLSSVEGKLEAFCSSITIFRAPNDISIEDKNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLE MENLKWRYL  FLKHNPS+TLDDL++ VVKSES+LRKCYE E 
Sbjct: 61  AKVSIGPFHHGAPHLEPMENLKWRYLSTFLKHNPSLTLDDLIELVVKSESRLRKCYEGEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           YDLD+DKFSQMMLLDCCFILELLLRYSKKR RR NDPVF TPGLL  LRCDL+LLENQIP
Sbjct: 121 YDLDSDKFSQMMLLDCCFILELLLRYSKKRFRRWNDPVFNTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL +VYE VQDPLEENMSLNDLTFRFF+TMVAG+R+FVYDNF V+ADHLLEMVHSCFL
Sbjct: 181 YFLLDEVYENVQDPLEENMSLNDLTFRFFKTMVAGDRKFVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYPR+ET+DKSKS ELP+ASKLKT GIKF+NARSPKS+LDIKFQ GVLEIP L+VY QT
Sbjct: 241 STYPRMETNDKSKSRELPSASKLKTAGIKFKNARSPKSLLDIKFQKGVLEIPPLRVYQQT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNL AYEI Q+GS  QVKSY++FMSHLLQSDEDVK+LCRR KIL DLED EE+II+
Sbjct: 301 EAILRNLAAYEIRQFGSDLQVKSYINFMSHLLQSDEDVKILCRR-KILIDLEDDEEQIIQ 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
           NLKWMR++KE LSGTYFAG+VQKLNEKP+RC+ +WR LRRNPV +GVAA+WV+++IFV A
Sbjct: 361 NLKWMREEKESLSGTYFAGIVQKLNEKPDRCLTQWRGLRRNPVAIGVAAVWVVVVIFVAA 420

Query: 421 FFSALTVLQRRYK 434
           FFSA+++LQRRYK
Sbjct: 421 FFSAISLLQRRYK 432

BLAST of Lag0021471 vs. NCBI nr
Match: XP_023526431.1 (UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526432.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526433.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526435.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526436.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526437.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526438.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo] >XP_023526439.1 UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.2 bits (1759), Expect = 3.0e-192
Identity = 348/433 (80.37%), Postives = 388/433 (89.61%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MDSSRALSHSID+PATSQGSSE+ESLLSSIEGKLEA CSSITIF+ P+EISIEDR+ FVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLESMENLKW YL AFLK+NPSV L  L++ VVKSES+LRKCYEEE 
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           Y  D+DKFSQ+MLLDCCFILELLLR+SKKRLRRRND VFTTPGLL  LRCDL+LLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL+DVYE VQDP EENMSLNDLTFRFF+T+V G+RQ VYDNFTV+ADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEENMSLNDLTFRFFKTLVVGDRQLVYDNFTVEADHLLEMVHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYPR+ET+DKSKS ELP+ASKLKT GIK +NARS KS+LDIKFQNGVLEIP LKVY +T
Sbjct: 241 STYPRVETNDKSKSRELPSASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNLVAYEI Q GS RQVKSY++FMSHLLQSD+DVK+L RR KIL D ED EE+II 
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRR-KILIDQEDDEEQIIR 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
           NLKWM  +KE LSGTYFAG+VQKLNEKP+RCVARWR+LRRNPV +G+ A+ V+++IFV A
Sbjct: 361 NLKWM-SEKESLSGTYFAGIVQKLNEKPDRCVARWRKLRRNPVAIGIVAVLVVVVIFVAA 420

Query: 421 FFSALTVLQRRYK 434
           FFSA +VLQRRYK
Sbjct: 421 FFSAFSVLQRRYK 431

BLAST of Lag0021471 vs. NCBI nr
Match: XP_022955709.1 (UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955710.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955711.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955712.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955713.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955714.1 UPF0481 protein At3g47200-like [Cucurbita moschata] >XP_022955716.1 UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 681.8 bits (1758), Expect = 3.9e-192
Identity = 348/433 (80.37%), Postives = 388/433 (89.61%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MDSSRALSHSID+PATSQGSSE+ESLLSSIEGKLEA CSSITIF+ P+EISIEDR+ FVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLESMENLKW YL AFLK+NPSV L  L++ VVKSES+LRKCYEEE 
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           Y  D+DKFSQ+MLLDCCFILELLLR+SKKRLRRRND VFTTPGLL  LRCDL+LLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL+DVYE VQDP EENMSLNDLTFRFF+TMVAG+RQ VYDNF V+ADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEENMSLNDLTFRFFKTMVAGDRQLVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYPR+ET+DKSKS ELPTASKLKT GIK +NARS KS+LDIKFQNGVLEIP LKVY +T
Sbjct: 241 STYPRVETNDKSKSKELPTASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNLVAYEI Q GS RQVKSY++FMSHLLQSD+DVK+L RR KIL+D ED EE+II 
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRR-KILNDQEDDEEQIIR 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
           NLKWM  ++E LSGTYFAG+VQKLNEKP+RCVARWR+LRR PV +G+ A+ V+++IFV A
Sbjct: 361 NLKWM-SERESLSGTYFAGIVQKLNEKPDRCVARWRKLRRTPVAIGIVAVLVVVVIFVAA 420

Query: 421 FFSALTVLQRRYK 434
           FFSA +VLQRRYK
Sbjct: 421 FFSAFSVLQRRYK 431

BLAST of Lag0021471 vs. NCBI nr
Match: KAG7018572.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681.0 bits (1756), Expect = 6.6e-192
Identity = 348/433 (80.37%), Postives = 389/433 (89.84%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MDSSRALSHSID+PATSQGSSE+ESLLSSIEGKLEA CSSITIF+ P+EISIEDR+ FVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
           +KVSIGPFHHGA HLESMENLKW YL AFLK+NPSV L  L++ VVKSES+LRKCYEEE 
Sbjct: 61  SKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           Y  D+DKFSQ+MLLDCCFILELLLR+SKKRLRRRND VFTTPGLL  LRCDL+LLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL+DVYE VQDP EE MSLNDLTFRFF+TMVAG+RQ VYDNF V+ADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEEIMSLNDLTFRFFKTMVAGDRQLVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYPR+ET+DKSKS ELPTASKLKT GIK +NARS KS+LDIKFQNGVLEIP LKVY +T
Sbjct: 241 STYPRVETNDKSKSKELPTASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNLVAYEI Q GS RQVKSY++FMSHLLQSD+DVK+L RR KIL+D ED EE+II 
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRR-KILNDQEDDEEQIIR 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
           NLKWM  ++E LSGTYFAG+VQKLNEKP+RCVARWR+LRRNPV +G+ A+ V+++IFV A
Sbjct: 361 NLKWM-SERESLSGTYFAGIVQKLNEKPDRCVARWRKLRRNPVAIGIVAVLVVVVIFVAA 420

Query: 421 FFSALTVLQRRYK 434
           FFSA +VLQRRYK
Sbjct: 421 FFSAFSVLQRRYK 431

BLAST of Lag0021471 vs. NCBI nr
Match: XP_022138112.1 (UPF0481 protein At3g47200-like [Momordica charantia] >XP_022138114.1 UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 638.3 bits (1645), Expect = 4.9e-179
Identity = 325/434 (74.88%), Postives = 377/434 (86.87%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           M+ SRALSH+IDIPA S+  S++ESLL S+E K+EA CSSI IFKVPDEISI++R  FVP
Sbjct: 1   MNPSRALSHAIDIPAISRERSDEESLLCSMEAKMEAFCSSIIIFKVPDEISIDNREVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLESME+LKW YL AFLKHNPSV LDDLL+FV KSES++RKCYE E 
Sbjct: 61  AKVSIGPFHHGAPHLESMEDLKWNYLCAFLKHNPSVGLDDLLEFVAKSESRVRKCYEVEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           +DLD+ KF++MM+LDCCF+LELLLR+S KRL+RRNDPVFTTPGLL+ L+ DLILLENQIP
Sbjct: 121 HDLDSQKFARMMVLDCCFVLELLLRFSIKRLKRRNDPVFTTPGLLLDLKSDLILLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLLR+VYE VQD  EENM LNDL FRFFRT+VAGERQ VYDNF  DADHLL++VHSCFL
Sbjct: 181 YFLLREVYEKVQDSREENMPLNDLAFRFFRTIVAGERQSVYDNFQQDADHLLDIVHSCFL 240

Query: 241 STYPRIET-DDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQ 300
           STYPRIET ++KSK+ ELP ASKLK+ GIKF+NA +PKSVLDIKFQNG LEIPTL+V   
Sbjct: 241 STYPRIETKNNKSKTAELPRASKLKSAGIKFKNAVTPKSVLDIKFQNGGLEIPTLEVSKH 300

Query: 301 TETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKII 360
           TETIL+NL+AYEICQ GSA+QVKSYV FMSHLLQSDED+K+LC R KIL +LE  E +II
Sbjct: 301 TETILKNLIAYEICQIGSAQQVKSYVDFMSHLLQSDEDMKLLCGR-KILINLEKDETQII 360

Query: 361 ENLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVM 420
            NLKWMR+QK +LSGTYFAGVVQKLNE P+R +  WRRLRRNPV +GV A+W L++IFV 
Sbjct: 361 ANLKWMRQQKANLSGTYFAGVVQKLNEPPDRFIVWWRRLRRNPVAIGVVAVWALVVIFVA 420

Query: 421 AFFSALTVLQRRYK 434
           AFFSAL++LQRRY+
Sbjct: 421 AFFSALSLLQRRYR 433

BLAST of Lag0021471 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-36
Identity = 132/438 (30.14%), Postives = 215/438 (49.09%), Query Frame = 0

Query: 17  SQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVPTKVSIGPFHHGAQHLE 76
           S GS E   LL S  GK E+ C    IF+VP+     +  A+ P  VSIGP+H+G +HL+
Sbjct: 28  SSGSKEPVLLLES-AGK-ESCC----IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQ 87

Query: 77  SMENLKWRYLYAFLKHNPSVTLDD--LLQFVVKSESQLRKCYEEECYDLDTDKFSQMMLL 136
            ++  K R L  FL       +++  L++ VV  E ++RK Y EE        F  MM+L
Sbjct: 88  MIQQHKPRLLQLFLDEAKKKDVEENVLVKAVVDLEDKIRKSYSEELKTGHDLMF--MMVL 147

Query: 137 DCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIPYFLLRDVYETVQDP 196
           D CFIL + L  S   +    DP+F+ P LL  ++ DL+LLENQ+P+F+L+ +Y  V   
Sbjct: 148 DGCFILMVFLIMS-GNIELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLY--VGSK 207

Query: 197 LEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL--------STYPRI 256
           +  +  LN + F FF+  +  E  +   +    A HLL+++   FL        ++ P +
Sbjct: 208 IGVSSDLNRIAFHFFKNPIDKEGSYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHV 267

Query: 257 ETD-DKSKSGELP-----------TASKLKTTGIKFRNARSPK-SVLDIKFQNGVLEIPT 316
           +    + KSG +P           +A +L+  GIKFR  RS + S+L+++ +   L+IP 
Sbjct: 268 QVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQ 327

Query: 317 LKVYSQTETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLED 376
           L+      +   N VA+E     S+ ++ +Y+ FM  LL ++EDV  L   K I+ +   
Sbjct: 328 LRFDGFISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFG 387

Query: 377 KEEKIIENLKWMRKQKE-DLSGTYFAGVVQKLNEKPERCV-ARWRRLRRNPVVVGVAALW 430
              ++ E  K + K    ++  +Y   V + +NE  ++     W   R         + W
Sbjct: 388 SNNEVSEFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRH----THFESPW 447

BLAST of Lag0021471 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.1e-11
Identity = 102/498 (20.48%), Postives = 180/498 (36.14%), Query Frame = 0

Query: 31  EGKLEAVCSSITIFKVPDEISIEDRSAFVPTKVSIGPFHHGAQHLESMENLKWRYLYAFL 90
           E  LE V  +++IF VP  +      ++ P +VSIGP+H     L  ME  K        
Sbjct: 35  EHDLEEV--TVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIR 94

Query: 91  KHNPSVTLDDLLQFVVKSESQLRKCYEEECYDLDTDKFSQMMLLDCCFILELLLRYSKKR 150
               S    DL++ +   E ++R CY  +    + +    +M +D  F++E L  YS ++
Sbjct: 95  NQYNSFRFHDLVEKLQSMEIKIRACY-HKYIGFNGETLLWIMAVDSSFLIEFLKIYSFRK 154

Query: 151 LRRRNDPVFTTPGLLVRLRCDLILLENQIPYFLLRDVYETVQDPLEENMSLNDLTFRFFR 210
           +    + +    G    LR D++++ENQIP F+LR   +T++  LE   S +DL      
Sbjct: 155 V----ETLINRVGHNEILR-DIMMIENQIPLFVLR---KTLEFQLESTESADDLLLSVLT 214

Query: 211 TMVAGERQFV--YDNFTV------DADHLLEMVHSCFLSTYPRIETDDKSKSGE------ 270
            +       V  +D+  +      + +H+L+ ++   +   PRIE ++  +  E      
Sbjct: 215 GLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIV---PRIEEEELEEDDEENRADE 274

Query: 271 ------------------------------------------------------------ 330
                                                                       
Sbjct: 275 NGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENE 334

Query: 331 ------------------------LPTASKLKTTGIKFR-NARSPKSVLDIKFQNGVLEI 390
                                   +P+ S L   G++F+  A    S +     +G   +
Sbjct: 335 ATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYL 394

Query: 391 PTLKVYSQTETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDL 430
           P + +   TET+LRNLVAYE            Y   ++ ++ S+EDV++L  +  ++S L
Sbjct: 395 PVINLDINTETVLRNLVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRL 454

BLAST of Lag0021471 vs. ExPASy TrEMBL
Match: A0A6J1GVU1 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111457630 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.9e-192
Identity = 348/433 (80.37%), Postives = 388/433 (89.61%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MDSSRALSHSID+PATSQGSSE+ESLLSSIEGKLEA CSSITIF+ P+EISIEDR+ FVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIEGKLEAFCSSITIFRAPNEISIEDRNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLESMENLKW YL AFLK+NPSV L  L++ VVKSES+LRKCYEEE 
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLVELVVKSESRLRKCYEEEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           Y  D+DKFSQ+MLLDCCFILELLLR+SKKRLRRRND VFTTPGLL  LRCDL+LLENQIP
Sbjct: 121 YGFDSDKFSQIMLLDCCFILELLLRFSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL+DVYE VQDP EENMSLNDLTFRFF+TMVAG+RQ VYDNF V+ADHLLEMVHSCFL
Sbjct: 181 YFLLKDVYENVQDPTEENMSLNDLTFRFFKTMVAGDRQLVYDNFMVEADHLLEMVHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYPR+ET+DKSKS ELPTASKLKT GIK +NARS KS+LDIKFQNGVLEIP LKVY +T
Sbjct: 241 STYPRVETNDKSKSKELPTASKLKTAGIKIKNARSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNLVAYEI Q GS RQVKSY++FMSHLLQSD+DVK+L RR KIL+D ED EE+II 
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRR-KILNDQEDDEEQIIR 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
           NLKWM  ++E LSGTYFAG+VQKLNEKP+RCVARWR+LRR PV +G+ A+ V+++IFV A
Sbjct: 361 NLKWM-SERESLSGTYFAGIVQKLNEKPDRCVARWRKLRRTPVAIGIVAVLVVVVIFVAA 420

Query: 421 FFSALTVLQRRYK 434
           FFSA +VLQRRYK
Sbjct: 421 FFSAFSVLQRRYK 431

BLAST of Lag0021471 vs. ExPASy TrEMBL
Match: A0A6J1CA62 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111009362 PE=4 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.4e-179
Identity = 325/434 (74.88%), Postives = 377/434 (86.87%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           M+ SRALSH+IDIPA S+  S++ESLL S+E K+EA CSSI IFKVPDEISI++R  FVP
Sbjct: 1   MNPSRALSHAIDIPAISRERSDEESLLCSMEAKMEAFCSSIIIFKVPDEISIDNREVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLESME+LKW YL AFLKHNPSV LDDLL+FV KSES++RKCYE E 
Sbjct: 61  AKVSIGPFHHGAPHLESMEDLKWNYLCAFLKHNPSVGLDDLLEFVAKSESRVRKCYEVEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           +DLD+ KF++MM+LDCCF+LELLLR+S KRL+RRNDPVFTTPGLL+ L+ DLILLENQIP
Sbjct: 121 HDLDSQKFARMMVLDCCFVLELLLRFSIKRLKRRNDPVFTTPGLLLDLKSDLILLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLLR+VYE VQD  EENM LNDL FRFFRT+VAGERQ VYDNF  DADHLL++VHSCFL
Sbjct: 181 YFLLREVYEKVQDSREENMPLNDLAFRFFRTIVAGERQSVYDNFQQDADHLLDIVHSCFL 240

Query: 241 STYPRIET-DDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQ 300
           STYPRIET ++KSK+ ELP ASKLK+ GIKF+NA +PKSVLDIKFQNG LEIPTL+V   
Sbjct: 241 STYPRIETKNNKSKTAELPRASKLKSAGIKFKNAVTPKSVLDIKFQNGGLEIPTLEVSKH 300

Query: 301 TETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKII 360
           TETIL+NL+AYEICQ GSA+QVKSYV FMSHLLQSDED+K+LC R KIL +LE  E +II
Sbjct: 301 TETILKNLIAYEICQIGSAQQVKSYVDFMSHLLQSDEDMKLLCGR-KILINLEKDETQII 360

Query: 361 ENLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVM 420
            NLKWMR+QK +LSGTYFAGVVQKLNE P+R +  WRRLRRNPV +GV A+W L++IFV 
Sbjct: 361 ANLKWMRQQKANLSGTYFAGVVQKLNEPPDRFIVWWRRLRRNPVAIGVVAVWALVVIFVA 420

Query: 421 AFFSALTVLQRRYK 434
           AFFSAL++LQRRY+
Sbjct: 421 AFFSALSLLQRRYR 433

BLAST of Lag0021471 vs. ExPASy TrEMBL
Match: A0A1S3AY98 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103483890 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.7e-175
Identity = 316/433 (72.98%), Postives = 370/433 (85.45%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MDSS  +SH+I+IP  SQ SS +ESLLSSIEGKLEA CSS+TIFK P EI+IE R+ FVP
Sbjct: 1   MDSSTPVSHTINIPGISQESSREESLLSSIEGKLEANCSSVTIFKAPSEINIEGRNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HL+S+ENLKWRYL  FLKHN S+TL DL++ VVKSES+L+KCYE++ 
Sbjct: 61  AKVSIGPFHHGAAHLQSVENLKWRYLSTFLKHNSSLTLQDLIKIVVKSESRLKKCYEKKF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
             LD D+FS +MLLDCCFILELLLRYSK+R +RRNDPVFTTPGLL  ++CDL+LLENQIP
Sbjct: 121 CSLDRDEFSLIMLLDCCFILELLLRYSKRRFKRRNDPVFTTPGLLFDIKCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL ++YE V DP EENM L+DLTFRFFRTMV G+R+F+ DNF V+ADHLLEMVHSCFL
Sbjct: 181 YFLLDEIYEKVLDPREENMFLSDLTFRFFRTMVPGDRKFMGDNFVVEADHLLEMVHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYP ++T+DK KS ELP+ASKLKT GIKF+NARS KS+LDIKFQNGVLEIP L+VY QT
Sbjct: 241 STYPPVKTNDKLKSKELPSASKLKTAGIKFKNARSSKSLLDIKFQNGVLEIPPLRVYQQT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNL AYEI Q G+ +QVKSY+ FMSHLLQSD DVK+LC RKKIL  LED EE+IIE
Sbjct: 301 EAILRNLAAYEIRQSGTDQQVKSYLKFMSHLLQSDGDVKILC-RKKILYALEDDEEQIIE 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
           NLKW+R+QKE LSGTYFAG+VQKLNEKP+R V RWRRLRR P  +GVAA  ++++IF  A
Sbjct: 361 NLKWIREQKESLSGTYFAGIVQKLNEKPDRSVVRWRRLRRKPTAIGVAADLMVVVIFGAA 420

Query: 421 FFSALTVLQRRYK 434
           FF+A ++LQRRYK
Sbjct: 421 FFAAFSILQRRYK 432

BLAST of Lag0021471 vs. ExPASy TrEMBL
Match: A0A6J1IWZ4 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111479248 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 5.3e-171
Identity = 313/392 (79.85%), Postives = 349/392 (89.03%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MDSSRALSHSID+PATSQGSSE+ESLLSSIE KLEA CSSITIF+  +EISIEDR+ FVP
Sbjct: 1   MDSSRALSHSIDVPATSQGSSEEESLLSSIERKLEAFCSSITIFRATNEISIEDRNVFVP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLESMENLKW YL AFLK+NPSV L  L++ VVKSES+LRKCYEEE 
Sbjct: 61  AKVSIGPFHHGAPHLESMENLKWCYLSAFLKNNPSVDLQYLIELVVKSESRLRKCYEEEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           Y  D++KFSQ+MLLDCCFILELLLRYSKKRLRRRND VFTTPGLL  LRCDL+LLENQIP
Sbjct: 121 YGFDSNKFSQIMLLDCCFILELLLRYSKKRLRRRNDSVFTTPGLLFDLRCDLMLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL+DVY  VQDP EENMSLNDLTFRFF+TMVAG+RQFVYDNF V+ADHLLEM+HSCFL
Sbjct: 181 YFLLKDVYANVQDPTEENMSLNDLTFRFFKTMVAGDRQFVYDNFMVEADHLLEMIHSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYPR+ET+D SKS ELP+ASKLKT GIK +N +S KS+LDIKFQNGVLEIP LKVY +T
Sbjct: 241 STYPRMETNDNSKSRELPSASKLKTAGIKIKNFKSSKSLLDIKFQNGVLEIPPLKVYQKT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           E ILRNLVAYEI Q GS RQVKSY++FMSHLLQSD+DVK+L RR KIL+D E+ EE+II 
Sbjct: 301 EVILRNLVAYEIHQSGSDRQVKSYINFMSHLLQSDQDVKILYRR-KILNDQENDEEQIIR 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCV 393
           NLKWMR +KE LSGTYFAG+VQKLN+K +RCV
Sbjct: 361 NLKWMR-EKESLSGTYFAGIVQKLNKKRDRCV 390

BLAST of Lag0021471 vs. ExPASy TrEMBL
Match: A0A0A0L821 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G166290 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.2e-167
Identity = 303/433 (69.98%), Postives = 362/433 (83.60%), Query Frame = 0

Query: 1   MDSSRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVP 60
           MD S  +SH+I+I   SQ S ++ESLLS IE KLEA CSS TI+K P EI+IEDR+ F+P
Sbjct: 1   MDPSTPVSHTINISGISQESFQEESLLSCIERKLEANCSSFTIYKAPSEINIEDRNVFLP 60

Query: 61  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 120
            KVSIGPFHHGA HLES+E LKW YL  FL H PS+TL DL++ VVKSES+ RKCYE+E 
Sbjct: 61  AKVSIGPFHHGAPHLESVEKLKWHYLSTFLTHKPSLTLQDLIKLVVKSESRGRKCYEKEF 120

Query: 121 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 180
           Y  D D+FSQ+MLLDCCFILELLLRY+K+R RR NDPVFTTPGLL  LRCDL+LLENQIP
Sbjct: 121 YSSDRDEFSQIMLLDCCFILELLLRYTKRRFRRPNDPVFTTPGLLYDLRCDLVLLENQIP 180

Query: 181 YFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFL 240
           YFLL ++Y  V D LEENM L+DLT RFFRTMV G+R+F+ DNF V+A+HLLEMV+SCFL
Sbjct: 181 YFLLEEIYAKVLDGLEENMYLSDLTSRFFRTMVPGDRKFIGDNFIVEANHLLEMVYSCFL 240

Query: 241 STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQT 300
           STYP +ET+DK KS ELP+ASKLK  GIKF+NARS KS+LDIKFQNGVLEIP L+VY +T
Sbjct: 241 STYPPVETNDKLKSKELPSASKLKAAGIKFKNARSSKSLLDIKFQNGVLEIPPLRVYQKT 300

Query: 301 ETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIE 360
           ETILRNL AYEICQ+G+  QVKSY++FMSHLLQSDEDVK+LC RKKIL+ L+D+EE+IIE
Sbjct: 301 ETILRNLAAYEICQFGTDLQVKSYLNFMSHLLQSDEDVKILC-RKKILNALKDEEEQIIE 360

Query: 361 NLKWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRRNPVVVGVAALWVLIMIFVMA 420
            LKW+R+QK+ LSGT+FAG+VQKL EKP+R VARWRRLR N   + VA + ++++IF  A
Sbjct: 361 KLKWIREQKDSLSGTFFAGIVQKLKEKPDRSVARWRRLRSNSTAISVATVLMVVVIFGAA 420

Query: 421 FFSALTVLQRRYK 434
           FF+A +VLQRRYK
Sbjct: 421 FFAAFSVLQRRYK 432

BLAST of Lag0021471 vs. TAIR 10
Match: AT5G22540.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 174.9 bits (442), Expect = 1.4e-43
Identity = 120/357 (33.61%), Postives = 186/357 (52.10%), Query Frame = 0

Query: 43  IFKVPDEISIEDRSAFVPTKVSIGPFHHGAQHLESMENLKWRYLYAFL--KHNPSVTLDD 102
           I ++P  ++  +  A+ P  VSIGP+HHG +HL+  +  K R+L  F+           +
Sbjct: 33  IVRIPQSLARINLKAYEPKIVSIGPYHHGKEHLKMTQQHKRRFLKFFVAKMEEKGFVPQE 92

Query: 103 LLQFVVKSESQLRKCYEEECYDLDTDKFSQMMLLDCCFILELLLRYS-KKRLRRRNDPVF 162
           L++ V   E  +R  Y E+   LD++   QMM+LD CFIL L    S K      +DP+F
Sbjct: 93  LVKAVSSLEGVIRGSYSED-LGLDSENLVQMMVLDGCFILTLFFVVSGKVEYTNLDDPIF 152

Query: 163 TTPGLLVRLRCDLILLENQIPYFLLRDVYETVQDPLEENMSLNDLTFRFFRTMVAGERQF 222
             P +L  +R DL+LLENQ+PY LL+ ++ET    L     LN++ F FF   +     F
Sbjct: 153 RMPWILPSIRADLLLLENQVPYVLLQTLFET--SKLVTCSGLNEIAFEFFNYSLQKPETF 212

Query: 223 VYDNFTVDADHLLEMVHSCFLST-YPRIETDDKSKS--------GELPTASKLKTTGIKF 282
              ++ ++A HLL+++   F+     R   D  SKS        G + +A KL   GIKF
Sbjct: 213 WEKHYGLEAKHLLDLIRKTFVPVPSQRRIKDHSSKSSFNDHEYLGFVLSAKKLHLRGIKF 272

Query: 283 RNARSPKSVLDIKFQNGVLEIPTLKVYSQTETILRNLVAYEICQYGSARQVKSYVSFMSH 342
           +  ++  S+LDI + NGVL IP + +   T +I  N VA+E     S+  + SYV+FM+ 
Sbjct: 273 KPRKNTDSILDISYSNGVLHIPPVVMDDFTASIFLNCVAFEQLYADSSNHITSYVAFMAC 332

Query: 343 LLQSDEDVKMLCRRKKILSDLEDKEEKIIENLKWMRKQ-KEDLSGTYFAGVVQKLNE 387
           L+  + D   L  R +IL +    E+++    K + K    DL  +Y A V + +NE
Sbjct: 333 LINEESDASFLSER-RILENYFGTEDEVSRFYKRIGKDIALDLEKSYLAKVFEGVNE 385

BLAST of Lag0021471 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-42
Identity = 124/439 (28.25%), Postives = 229/439 (52.16%), Query Frame = 0

Query: 4   SRALSHSIDIPATSQGSSEKESLLSSIEGKLEAVCSSIT---IFKVPDEISIEDRSAFVP 63
           S    H +         +E ++L+ SI+ KL  + S  T   I+KVP+++   +  A+ P
Sbjct: 253 SEVQCHRLCFAYERMNQNEGDALVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTP 312

Query: 64  TKVSIGPFHHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEEC 123
             VS GP H G + L++ME+ K+RYL +F+    S +L+DL++     E   R CY E+ 
Sbjct: 313 RLVSFGPLHRGKEELQAMEDQKYRYLLSFIPRTNS-SLEDLVRLARTWEQNARSCYAED- 372

Query: 124 YDLDTDKFSQMMLLDCCFILELLLRYSKKRLRRRNDPVFTTPGLLVRLRCDLILLENQIP 183
             L +D+F +M+++D  F++ELLLR    RLR  ND +F    ++  +  D+IL+ENQ+P
Sbjct: 373 VKLHSDEFVEMLVVDGSFLVELLLRSHYPRLRGENDRIFGNSMMITDVCRDMILIENQLP 432

Query: 184 YFLLRDVYETVQDPLEEN----MSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVH 243
           +F++++++  + +  ++     + L    F +F + +  E+      F  + +H ++++ 
Sbjct: 433 FFVVKEIFLLLLNYYQQGTPSIIQLAQRHFSYFLSRIDDEK------FITEPEHFVDLLR 492

Query: 244 SCFLSTYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKV 303
           SC+L  +P        K    P A++L T G++F+ A +   +LDI F +GVL+IPT+ V
Sbjct: 493 SCYLPQFPIKLEYTTVKVDNAPEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVV 552

Query: 304 YSQTETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEE 363
              TE++ +N++ +E C+  S +    Y+  +   ++S  D  +L     I++ L +  +
Sbjct: 553 DDLTESLYKNIIGFEQCRC-SNKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNSVD 612

Query: 364 KIIENL-KWMRKQKEDLSGTYFAGVVQKLNEKPERCVARWRRLRR-----NPVVVG--VA 423
             + NL   + K+       YF+ + + L         RW+ + R     NP  V    A
Sbjct: 613 --VSNLFNSISKEVIYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFA 672

Query: 424 ALWVLIMIFVMAFFSALTV 428
           AL +L++ F+ +  S L +
Sbjct: 673 ALLLLLLTFIQSVCSILAL 680

BLAST of Lag0021471 vs. TAIR 10
Match: AT3G47210.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 170.6 bits (431), Expect = 2.7e-42
Identity = 130/393 (33.08%), Postives = 201/393 (51.15%), Query Frame = 0

Query: 21  SEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVPTKVSIGPFHHGAQHLESMEN 80
           SEK  LL    GK E+ C    IF+VP   +  +  A+ P  VSIGP+HHG +HLE ++ 
Sbjct: 78  SEKRVLLLESAGK-ESCC----IFRVPKSFAEMNPEAYKPKVVSIGPYHHGRKHLEMIQQ 137

Query: 81  LKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEECYDLDTDKFSQMMLLDCCFIL 140
            K R+L+ FL+   SV  D L   VV  E ++RK Y E   +    +   MM+LD CFIL
Sbjct: 138 HKLRFLHLFLR-TASVDRDVLFNAVVDWEDEIRKSYSEG-LEGSPHELVYMMILDGCFIL 197

Query: 141 ELLLRYSKK-RLRRRNDPVFTTPGLLVRLRCDLILLENQIPYFLLRDVYETVQDPLEENM 200
            LLL  S+K  L    DP+ T P +L  ++ DL+LLENQ+P+F+L+ +++  +  +    
Sbjct: 198 MLLLIVSRKIELYESEDPILTIPWILPSIQSDLLLLENQVPFFVLQTLFD--KSEIGVPG 257

Query: 201 SLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFLS-------TYPRIETDDKS 260
            LN + F FF   +    ++   +   +A HLL+++   FL           + ++  KS
Sbjct: 258 DLNRMAFSFFNLSMDKPERYWVKHRNFNAKHLLDLIRMSFLPMDGYEDFQLTKGKSRKKS 317

Query: 261 KSG--ELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKVYSQTETILRNLVAY 320
            SG   L +A++L   GI F       S+LDI+ +   L+IP L++     +IL N VA+
Sbjct: 318 SSGLTLLLSATRLSLQGIDFSLRSGADSMLDIRLKKNRLQIPVLRLDGFIISILLNCVAF 377

Query: 321 EICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEEKIIENLKWMRKQKE 380
           E     S   + SYV FM  LL   ED   L RR +I+ +    E+++ +  K + K   
Sbjct: 378 EQFYAKSTNHITSYVVFMGCLLNGKEDATFLSRR-RIIENYFGSEKEVSKFFKTICKDVV 437

Query: 381 -DLSGTYFAGVVQKLNEKPERCVARWRRLRRNP 403
            D+  +Y   V  ++NE   +  +  R    +P
Sbjct: 438 FDIHASYLRNVFVEINENTSKWYSICRSFLLSP 460

BLAST of Lag0021471 vs. TAIR 10
Match: AT3G50180.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-41
Identity = 113/347 (32.56%), Postives = 183/347 (52.74%), Query Frame = 0

Query: 18  QGSSEKE-SLLSSIEGKLEAV--------CSSITIFKVPDEISIEDRSAFVPTKVSIGPF 77
           +GS  K    + SI+ KLE             + I+KVP  +   D+ ++ P  VS+GP+
Sbjct: 146 EGSGTKRLEWVISIKDKLEQAYRNDDRTSWGKLCIYKVPHYLHGNDKKSYFPQTVSLGPY 205

Query: 78  HHGAQHLESMENLKWRYLYAFLKHNPSVTLDDLLQFVVKSESQLRKCYEEECYDLDTDKF 137
           HHG Q  +SME  KWR +   LK   +  ++  L  +++ E + R CYE     L +++F
Sbjct: 206 HHGRQQTQSMECHKWRAVNMVLKRT-NQGIEVFLDAMIELEEKARACYEGSIV-LSSNEF 265

Query: 138 SQMMLLDCCFILELLLRYSKKRLR---RRNDPVFTTPGLLVRLRCDLILLENQIPYFLLR 197
           ++M+LLD CFILELL   ++  L+     NDPVF   G +  ++ D+I+LENQ+P F+L 
Sbjct: 266 TEMLLLDGCFILELLQGVNEGFLKLGYDHNDPVFAVRGSMHSIQRDMIMLENQLPLFVLN 325

Query: 198 DVYETVQDPLEENMSLNDLTFRFFRTMVAGERQFVYDN----FTVDADHLLEMVHSCFL- 257
            + E +Q   +    L +L  RFF  ++        ++     +    H L++ H   L 
Sbjct: 326 RLLE-LQPGTQNQTGLVELVVRFFIPLMPTAETLTENSPPRGVSNGELHCLDVFHRSLLF 385

Query: 258 ------STYPRIETDDKSKSGELPTASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTL 317
                 + Y R+   DK     +PT ++L+  G KF+  ++ +   DIKF NG LEIP L
Sbjct: 386 PRSSGKANYSRVA--DKHLQRVIPTVTELRDAGFKFKLNKTDR-FWDIKFSNGYLEIPGL 445

Query: 318 KVYSQTETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKML 342
            ++  T+++  NL+A+E C   S+  + SY+ FM +L+ S ED+  L
Sbjct: 446 LIHDGTKSLFLNLIAFEQCHIESSNDITSYIIFMDNLIDSPEDISYL 486

BLAST of Lag0021471 vs. TAIR 10
Match: AT3G47250.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-40
Identity = 130/438 (29.68%), Postives = 207/438 (47.26%), Query Frame = 0

Query: 20  SSEKESLLSSIEGKLEAVCSSITIFKVPDEISIEDRSAFVPTKVSIGPFHHGAQHLESME 79
           SS K  +L    GK     +S  IF++PD ++  +  A+ P  VSIGP+H+G  HL+ ++
Sbjct: 45  SSGKPLILLESAGK-----ASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQ 104

Query: 80  NLKWRYLYAFLKHNPSVTLDD--LLQFVVKSESQLRKCYEEECYDLDTDKFSQMMLLDCC 139
             K+R+L  F+       +D+  L   V   + ++R  Y EE   ++  +   MM+LD C
Sbjct: 105 QHKFRFLELFVDRATKKGMDENVLYAAVGALQHKIRASYSEE-LRIEKSELVSMMILDGC 164

Query: 140 FILELLLRYSKK-RLRRRNDPVFTTPGLLVRLRCDLILLENQIPYFLLRDVYETVQDPLE 199
           FIL LLL  S+K  L    DP+FT P +L  ++ DL+LLENQ+P+F+LR +++  +  + 
Sbjct: 165 FILMLLLIVSRKIDLDMNKDPIFTIPWILASIQSDLLLLENQVPFFVLRTIFD--KSGIG 224

Query: 200 ENMSLNDLTFRFFRTMVAGERQFVYDNFTVDADHLLEMVHSCFLSTYPRIETDD------ 259
               LN + F FF   +     +   +    A HLL++    F+ +   +   +      
Sbjct: 225 SPGDLNRMAFSFFNLSIDKPDTYWAKHRDRGAKHLLDLNRKTFIPSMRSMGESETTPSSK 284

Query: 260 ----KSKSGELP----------TASKLKTTGIKFRNARSPKSVLDIKFQNGVLEIPTLKV 319
               K KSGE+           +A +L+  GIKFR     +S+LDIK +   L+IP L++
Sbjct: 285 FQHSKGKSGEVSSSESTFPLILSAKRLRLQGIKFRLRSDAESILDIKLKKNKLQIPLLRL 344

Query: 320 YSQTETILRNLVAYEICQYGSARQVKSYVSFMSHLLQSDEDVKMLCRRKKILSDLEDKEE 379
                +I  N VA+E     S   + SYV FM  LL   ED   L   K+I+ +    E 
Sbjct: 345 DGFISSIFLNCVAFEQFYTESTNDITSYVVFMGCLLNDQEDATFLNNDKRIIENYFGNEN 404

Query: 380 KIIENLKWMRKQKE-DLSGTYFAGVVQKLNEKPERCV-ARWRRLRRNPVVVGVAALWVLI 433
           ++ +  K + K    D   +Y   V + +NE   +     W   R         + W  +
Sbjct: 405 EVSQFFKTICKDVVFDTRRSYLRNVFEGVNEYTSKTYNGVWAGFRH----THFESPWTAL 464