Lag0021468 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021468
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionubiquitin-conjugating enzyme E2 32
Locationchr7: 8082241 .. 8084414 (+)
RNA-Seq ExpressionLag0021468
SyntenyLag0021468
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGATAAGTACAATCTCAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGTCTAATCCTTCTGACGATTTCATGAGTCTGCCTCTTGAGGTTTGTTGCTTTTGAGTTTCGCTGGATTTCCATTTCTGTTTTGAAATTTACCTTTAGTGGGTTTTTGTTTTTGTTCCTGTTTGGTCTGTTTTCCATTCTAATTGTTTGCGTTTCTTTGTTGAATGATTGTGATTGTTCCCGATTATTGAATTGGTTTTGATGCATGTAGAATTGTGGTTGGAAATCGATGATTAATTTGGCGTTCTTTGATTTTTCTTACCTGCTGGTGTCTGCTGTTATTAGCATTTGAATGTTCTAATAGGGCTCAAAAAGGAAGAAAAATGGAAATATTAACGAGTTTTTGTTAAATTTTTGTTATGGAATTCATTCGAATTTATAATCTTCGAAAAATTTTCGATAGCGGAAAATTTGGGGCAGTCCTTTGTTTAAAAGCTTTACTCTTAGGTTAATAATTGCTTAGGTATCTCGACCGGATCCGGAATGTGTATCAATCTTCGTGCATCTTATGCTATTTTCATGTTCCTTGATTATTATAACTGTATTAACTTGACTGCATTTGGGAAAAAGAAAGAAAAAAAAAACAGCTATTGAAATCGTTGCTTGATTCATATACCAGTTCTGCATAATTTTATTTTTTTTAATTACTATAGGATCTAAGGCTTTTGCCAAACATCTTTCTATTGAATTTAAGGCTTGTGTTTCTTTTTCAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCCGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGTTAACCGTAAGATAGCAATCTCTTATTCTTACTTGTTTCCTTTTCTGTTGATTCTCTTCTGTTGTGTTCTTTGTAAGTGATTTTATACTTCATTGATGAGCTGATCATTGCCAAGTCCTTTTTTTCAGCCAAATGGACGCTTTGAAACCCAGACGAAGATTTGCTTAAGCATATCGAATCATCATCCTGAGCATTGGCAACCCTCATGGAGTGGTGAGTTCTTTGTATTATTTATTTCACCTGTCTTGTTACATTAGCTCATTAGATGTCCGTCATCTGGTTTCTGCATTTACATTTAGGGCATGTTTGGGTGTGATTTTAATAATAATGATCTGAACGAAAATGATTTGTTCATGAGAAGAGTGTTTTTCATATTATCATTAGAAGTTTGATTATATACTTTTAAAAGTGATTTTCATGAATACTTTGTTCTTAAAATCAAAGTGATTTCGTCCTTCCAAAATCACCCCAAACACGTCCTTTGTTACTTCGGGTTCCACATGCTCTGATCTCTTTTCTCTTTTCCCCGTCCCCCATTTTTGTTGATTGCTGTTTGTAGTGCGCACTGCCTTGGTTGCACTAATTGCTTTCATGCCCACCAACCCAAATGGCGCTCTTGGCTCCCTGGACTACAAGAAGGAGGAAAGACGGGTATTGGCCATCAAATCTCGAGATGCACCCCCCAAATTCGGTACTCCTGAACGTCAAAAGCTAATCGACGAGGTACTGCTGAGTTCTGTTGATATTTCATAAGCTGTTGAGAAGTACATTGAATTTCCCACATGTGATATGATATTGTTCATTCATGAGAGCTTCTTTACATCACAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCTGTCCCTCAACCGGGTTCTTCAGACGTTTCCGAGGACAAACCTGCTAACAAAGAGGCTGATGTCCAGGCAACTTCTGAATCTGCTGAACTTGCAGCTGCTGCTGGGGAAGAGCTGCAGAATCCACCTGCAAGCAACAGGATCGTCGAGGAAGCAGTACCAGAGGAGATATCTATGAATGCCAATGTCCGACCAGAAGCAGCAAGAGCAGCAAGAGAGGTGCCATCTAGTCGCGCCACCGCTCAGGTGCAGCGGAGGCCGGAGACTAGAGTTAGTAAACCCGCTGATGACCGGTTGTTCACATGGGCTGCTATAGGACTCACTCTTGCAATTGTTGTTCTCTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTCCTGTCTTCATGGACGAATCTTAG

mRNA sequence

ATGGCGGAGGATAAGTACAATCTCAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGTCTAATCCTTCTGACGATTTCATGAGTCTGCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCCGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGTTAACCCCAAATGGACGCTTTGAAACCCAGACGAAGATTTGCTTAAGCATATCGAATCATCATCCTGAGCATTGGCAACCCTCATGGAGTGTGCGCACTGCCTTGGTTGCACTAATTGCTTTCATGCCCACCAACCCAAATGGCGCTCTTGGCTCCCTGGACTACAAGAAGGAGGAAAGACGGGTATTGGCCATCAAATCTCGAGATGCACCCCCCAAATTCGGTACTCCTGAACGTCAAAAGCTAATCGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCTGTCCCTCAACCGGGTTCTTCAGACGTTTCCGAGGACAAACCTGCTAACAAAGAGGCTGATGTCCAGGCAACTTCTGAATCTGCTGAACTTGCAGCTGCTGCTGGGGAAGAGCTGCAGAATCCACCTGCAAGCAACAGGATCGTCGAGGAAGCAGTACCAGAGGAGATATCTATGAATGCCAATGTCCGACCAGAAGCAGCAAGAGCAGCAAGAGAGGTGCCATCTAGTCGCGCCACCGCTCAGGTGCAGCGGAGGCCGGAGACTAGAGTTAGTAAACCCGCTGATGACCGGTTGTTCACATGGGCTGCTATAGGACTCACTCTTGCAATTGTTGTTCTCTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTCCTGTCTTCATGGACGAATCTTAG

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATCTCAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGTCTAATCCTTCTGACGATTTCATGAGTCTGCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCCGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGTTAACCCCAAATGGACGCTTTGAAACCCAGACGAAGATTTGCTTAAGCATATCGAATCATCATCCTGAGCATTGGCAACCCTCATGGAGTGTGCGCACTGCCTTGGTTGCACTAATTGCTTTCATGCCCACCAACCCAAATGGCGCTCTTGGCTCCCTGGACTACAAGAAGGAGGAAAGACGGGTATTGGCCATCAAATCTCGAGATGCACCCCCCAAATTCGGTACTCCTGAACGTCAAAAGCTAATCGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCTGTCCCTCAACCGGGTTCTTCAGACGTTTCCGAGGACAAACCTGCTAACAAAGAGGCTGATGTCCAGGCAACTTCTGAATCTGCTGAACTTGCAGCTGCTGCTGGGGAAGAGCTGCAGAATCCACCTGCAAGCAACAGGATCGTCGAGGAAGCAGTACCAGAGGAGATATCTATGAATGCCAATGTCCGACCAGAAGCAGCAAGAGCAGCAAGAGAGGTGCCATCTAGTCGCGCCACCGCTCAGGTGCAGCGGAGGCCGGAGACTAGAGTTAGTAAACCCGCTGATGACCGGTTGTTCACATGGGCTGCTATAGGACTCACTCTTGCAATTGTTGTTCTCTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTCCTGTCTTCATGGACGAATCTTAG

Protein sequence

MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQPGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVRPEAARAAREVPSSRATAQVQRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGPVFMDES
Homology
BLAST of Lag0021468 vs. NCBI nr
Match: XP_038895230.1 (ubiquitin-conjugating enzyme E2 32 [Benincasa hispida])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 276/311 (88.75%), Postives = 284/311 (91.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GSSDVS +KP N+EADVQ TSE AEL AA+GEELQNPPA++R+VEE VPE       VR
Sbjct: 181 SGSSDVSGEKPTNEEADVQVTSEPAEL-AASGEELQNPPANDRVVEEVVPE-------VR 240

Query: 241 PE-AARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKA 300
           PE  AR AREVPSSR  AQ  QRR ET V KPADDRLFTWAAIGLTLAIVVLLLKKFMKA
Sbjct: 241 PEVVARVAREVPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKA 300

Query: 301 SAHGPVFMDES 310
           SAHG VFMDES
Sbjct: 301 SAHGSVFMDES 301

BLAST of Lag0021468 vs. NCBI nr
Match: XP_022138234.1 (ubiquitin-conjugating enzyme E2 32 [Momordica charantia])

HSP 1 Score: 522.7 bits (1345), Expect = 2.2e-144
Identity = 269/310 (86.77%), Postives = 280/310 (90.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAE+KYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPSDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFG+PERQKLIDEIHEY+LSKAPP+P P 
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGSPERQKLIDEIHEYILSKAPPIPQPS 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
           P             +ADVQ TSE AEL AAAGEELQNPPA +RIVEEAVPEE++ NAN +
Sbjct: 181 P------------SDADVQVTSEPAEL-AAAGEELQNPPAGDRIVEEAVPEEVTTNANPQ 240

Query: 241 PEAARAAREVPSSR-ATAQVQRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE  R AREVPSSR ATAQVQ R ETRV KPADDRLFTWAA+GLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVVRVAREVPSSRAATAQVQMRAETRVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. NCBI nr
Match: KAA0049541.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa])

HSP 1 Score: 521.2 bits (1341), Expect = 6.3e-144
Identity = 273/310 (88.06%), Postives = 282/310 (90.97%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GSSDVS++KP N+E+DVQ TSE AEL AAAGEELQNPP  NRIVEEAVPE       V+
Sbjct: 181 SGSSDVSDEKPTNEESDVQVTSEPAEL-AAAGEELQNPPTINRIVEEAVPE-------VQ 240

Query: 241 PEAARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE AR A   PSSR T Q  Q+RPETRV KPADDRLFTWAAIGLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVARVA---PSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. NCBI nr
Match: XP_008438945.1 (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 273/310 (88.06%), Postives = 281/310 (90.65%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GSSDVS+ KP N+E+DVQ TSE AEL AAAGEELQNPP  NRIVEEAVPE       V+
Sbjct: 181 SGSSDVSDQKPTNEESDVQVTSEPAEL-AAAGEELQNPPTINRIVEEAVPE-------VQ 240

Query: 241 PEAARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE AR A   PSSR T Q  Q+RPETRV KPADDRLFTWAAIGLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVARVA---PSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. NCBI nr
Match: XP_004134243.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical protein Csa_009813 [Cucumis sativus])

HSP 1 Score: 516.9 bits (1330), Expect = 1.2e-142
Identity = 273/310 (88.06%), Postives = 280/310 (90.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GS+DVS +KP N+EADVQ TSE+AELAAA  EELQN P  NRIVEEAVPE       V+
Sbjct: 181 SGSTDVSGEKPTNEEADVQVTSETAELAAAV-EELQNSPTINRIVEEAVPE-------VQ 240

Query: 241 PEAARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE AR A   PSSR T Q  Q RPETRV KPADDRLFTWAAIGLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVARVA---PSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. ExPASy Swiss-Prot
Match: Q9LSP7 (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 5.4e-106
Identity = 199/313 (63.58%), Postives = 237/313 (75.72%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPT+PNGALGS+DY K+ERR LAIKSR+ PPK+G+PERQK+IDEIH+Y+LSKA  VP P 
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
           P   + S+      EA  Q   + A        E ++   ++ IV++ + EE +   N  
Sbjct: 181 P--LECSQAPSIVSEAHSQVEPQEAITVV----EERSIATTDTIVDDQIIEETAEAVNTA 240

Query: 241 PEAARAAREVPSSRATAQV-----QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKF 300
                AA  +P+     +      QR       KP DDRLFTWAA+GLT+AI+VLLLKKF
Sbjct: 241 ASVVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKF 300

Query: 301 MKASAHGPVFMDE 309
           +K++ +   FMD+
Sbjct: 301 IKSNGYSTGFMDD 307

BLAST of Lag0021468 vs. ExPASy Swiss-Prot
Match: Q9Y385 (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 4.8e-54
Identity = 95/146 (65.07%), Postives = 118/146 (80.82%), Query Frame = 0

Query: 3   EDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRD 149
           T   GA+GSLDY  EERR LA KS+D
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

BLAST of Lag0021468 vs. ExPASy Swiss-Prot
Match: Q9JJZ4 (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 4.8e-54
Identity = 95/146 (65.07%), Postives = 118/146 (80.82%), Query Frame = 0

Query: 3   EDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRD 149
           T   GA+GSLDY  EERR LA KS+D
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

BLAST of Lag0021468 vs. ExPASy Swiss-Prot
Match: Q6FQK7 (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-33
Identity = 61/135 (45.19%), Postives = 91/135 (67.41%), Query Frame = 0

Query: 12  AVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K M  NP    ++ P EENI EW + I GP D+ ++GG YHG +  P++YP
Sbjct: 6   AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           +KPP+  ++TPNGRF+  T++CLS+S++HP+ W P WSV T L  L++FM T      GS
Sbjct: 66  YKPPAIRMITPNGRFKENTRLCLSMSDYHPDTWNPGWSVATILNGLLSFM-TGDESTTGS 125

Query: 132 LDYKKEERRVLAIKS 147
           +   ++E+++LA KS
Sbjct: 126 ITTTQQEKKILAKKS 139

BLAST of Lag0021468 vs. ExPASy Swiss-Prot
Match: Q54AK6 (Ubiquitin-conjugating enzyme E2 J2-like OS=Dictyostelium discoideum OX=44689 GN=DDB_G0294380 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.6e-31
Identity = 62/152 (40.79%), Postives = 96/152 (63.16%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVK----RILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFE 60
           M+E   ++ +P  K    R+ +E +E+  NP ++ +  P   NI EW + I G  ++ +E
Sbjct: 1   MSEHNSDIVHPPTKDCITRLKREFQEISKNPIENILVTPSPSNILEWHYVILGASNTPYE 60

Query: 61  GGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVA 120
           GG+Y+G++     YP  PPS ++ TP+GRFETQ ++CLSIS++HPE W PSWS  + L+ 
Sbjct: 61  GGVYYGQLIFKYNYPLSPPSILMTTPSGRFETQKRLCLSISDYHPESWSPSWSTSSILLG 120

Query: 121 LIAFMPTNPNGALGSLDYKKEERRVLAIKSRD 149
           L++FM  N   A GS+    +E+R+LA KS D
Sbjct: 121 LLSFMSDNEVTA-GSIVTTNDEKRILATKSMD 151

BLAST of Lag0021468 vs. ExPASy TrEMBL
Match: A0A6J1C9I9 (ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC111009459 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 269/310 (86.77%), Postives = 280/310 (90.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAE+KYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPSDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFG+PERQKLIDEIHEY+LSKAPP+P P 
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGSPERQKLIDEIHEYILSKAPPIPQPS 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
           P             +ADVQ TSE AEL AAAGEELQNPPA +RIVEEAVPEE++ NAN +
Sbjct: 181 P------------SDADVQVTSEPAEL-AAAGEELQNPPAGDRIVEEAVPEEVTTNANPQ 240

Query: 241 PEAARAAREVPSSR-ATAQVQRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE  R AREVPSSR ATAQVQ R ETRV KPADDRLFTWAA+GLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVVRVAREVPSSRAATAQVQMRAETRVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. ExPASy TrEMBL
Match: A0A5A7U7K5 (Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001470 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 273/310 (88.06%), Postives = 282/310 (90.97%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GSSDVS++KP N+E+DVQ TSE AEL AAAGEELQNPP  NRIVEEAVPE       V+
Sbjct: 181 SGSSDVSDEKPTNEESDVQVTSEPAEL-AAAGEELQNPPTINRIVEEAVPE-------VQ 240

Query: 241 PEAARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE AR A   PSSR T Q  Q+RPETRV KPADDRLFTWAAIGLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVARVA---PSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. ExPASy TrEMBL
Match: A0A1S3AXJ7 (ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.2e-144
Identity = 273/310 (88.06%), Postives = 281/310 (90.65%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GSSDVS+ KP N+E+DVQ TSE AEL AAAGEELQNPP  NRIVEEAVPE       V+
Sbjct: 181 SGSSDVSDQKPTNEESDVQVTSEPAEL-AAAGEELQNPPTINRIVEEAVPE-------VQ 240

Query: 241 PEAARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE AR A   PSSR T Q  Q+RPETRV KPADDRLFTWAAIGLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVARVA---PSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. ExPASy TrEMBL
Match: A0A0A0L954 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.7e-143
Identity = 273/310 (88.06%), Postives = 280/310 (90.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKKEERRVLAIKSR+APPKFGTPERQKLIDEIHEYMLSKAP  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
            GS+DVS +KP N+EADVQ TSE+AELAAA  EELQN P  NRIVEEAVPE       V+
Sbjct: 181 SGSTDVSGEKPTNEEADVQVTSETAELAAAV-EELQNSPTINRIVEEAVPE-------VQ 240

Query: 241 PEAARAAREVPSSRATAQV-QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 300
           PE AR A   PSSR T Q  Q RPETRV KPADDRLFTWAAIGLTLAIVVLLLKKFMKAS
Sbjct: 241 PEVARVA---PSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297

Query: 301 AHGPVFMDES 310
           AHG VFMDES
Sbjct: 301 AHGSVFMDES 297

BLAST of Lag0021468 vs. ExPASy TrEMBL
Match: A0A6J1GUE3 (ubiquitin-conjugating enzyme E2 32-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457632 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.1e-141
Identity = 261/309 (84.47%), Postives = 279/309 (90.29%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNL NPAVKRILQEVKE+QSNPSDDF SLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNLNNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPTNPNGALGSLDYKK+ERR LAIKSR+APPKFGTPERQKLI+EIHEY+LSK P  PVPQ
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPKFGTPERQKLINEIHEYLLSKVP--PVPQ 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
           PGSS+ SE+KPAN    VQ TS +AE+AAAA  E+ NPPA + IVEEA PE +S+N N++
Sbjct: 181 PGSSNASEEKPAN----VQVTSGAAEVAAAAVVEIPNPPAIDMIVEEAAPEGLSVNVNLQ 240

Query: 241 PEAARAAREVPSSRATAQVQRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKFMKASA 300
           PEAAR AREVPSSR TAQVQ+RPET   +PA+D LF WAAIGLTLAIVVLLLKKFMKASA
Sbjct: 241 PEAARVAREVPSSRPTAQVQQRPETGAHRPANDWLFKWAAIGLTLAIVVLLLKKFMKASA 300

Query: 301 HGPVFMDES 310
           HG VFMDES
Sbjct: 301 HGSVFMDES 303

BLAST of Lag0021468 vs. TAIR 10
Match: AT3G17000.1 (ubiquitin-conjugating enzyme 32 )

HSP 1 Score: 385.6 bits (989), Expect = 3.8e-107
Identity = 199/313 (63.58%), Postives = 237/313 (75.72%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSRDAPPKFGTPERQKLIDEIHEYMLSKAPPVPVPQ 180
           MPT+PNGALGS+DY K+ERR LAIKSR+ PPK+G+PERQK+IDEIH+Y+LSKA  VP P 
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 PGSSDVSEDKPANKEADVQATSESAELAAAAGEELQNPPASNRIVEEAVPEEISMNANVR 240
           P   + S+      EA  Q   + A        E ++   ++ IV++ + EE +   N  
Sbjct: 181 P--LECSQAPSIVSEAHSQVEPQEAITVV----EERSIATTDTIVDDQIIEETAEAVNTA 240

Query: 241 PEAARAAREVPSSRATAQV-----QRRPETRVSKPADDRLFTWAAIGLTLAIVVLLLKKF 300
                AA  +P+     +      QR       KP DDRLFTWAA+GLT+AI+VLLLKKF
Sbjct: 241 ASVVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKF 300

Query: 301 MKASAHGPVFMDE 309
           +K++ +   FMD+
Sbjct: 301 IKSNGYSTGFMDD 307

BLAST of Lag0021468 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 123.6 bits (309), Expect = 2.7e-28
Identity = 55/134 (41.04%), Postives = 81/134 (60.45%), Query Frame = 0

Query: 13  VKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKS 147
           +    E++ LA  S
Sbjct: 127 NTSVAEKQRLAKSS 139

BLAST of Lag0021468 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 123.6 bits (309), Expect = 2.7e-28
Identity = 55/134 (41.04%), Postives = 81/134 (60.45%), Query Frame = 0

Query: 13  VKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKS 147
           +    E++ LA  S
Sbjct: 127 NTSVAEKQRLAKSS 139

BLAST of Lag0021468 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-27
Identity = 69/199 (34.67%), Postives = 107/199 (53.77%), Query Frame = 0

Query: 13  VKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSRDAPPKFGTPERQKL----IDEIHEYMLSKAPPVPVPQPGSSDVS 192
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++     +  P S   S
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

Query: 193 EDKPANKEADVQATSESAE 207
           + K    E  +  T   +E
Sbjct: 187 DTK-VESEKTIDPTKGDSE 200

BLAST of Lag0021468 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-27
Identity = 69/199 (34.67%), Postives = 107/199 (53.77%), Query Frame = 0

Query: 13  VKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSRDAPPKFGTPERQKL----IDEIHEYMLSKAPPVPVPQPGSSDVS 192
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++     +  P S   S
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

Query: 193 EDKPANKEADVQATSESAE 207
           + K    E  +  T   +E
Sbjct: 187 DTK-VESEKTIDPTKGDSE 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895230.11.3e-14488.75ubiquitin-conjugating enzyme E2 32 [Benincasa hispida][more]
XP_022138234.12.2e-14486.77ubiquitin-conjugating enzyme E2 32 [Momordica charantia][more]
KAA0049541.16.3e-14488.06ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiqui... [more]
XP_008438945.11.1e-14388.06PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo][more]
XP_004134243.11.2e-14288.06ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q9LSP75.4e-10663.58Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2... [more]
Q9Y3854.8e-5465.07Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2[more]
Q9JJZ44.8e-5465.07Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2[more]
Q6FQK72.7e-3345.19Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 13... [more]
Q54AK62.6e-3140.79Ubiquitin-conjugating enzyme E2 J2-like OS=Dictyostelium discoideum OX=44689 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1C9I91.0e-14486.77ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC11100945... [more]
A0A5A7U7K53.0e-14488.06Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXJ75.2e-14488.06ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 ... [more]
A0A0A0L9545.7e-14388.06UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE... [more]
A0A6J1GUE39.1e-14184.47ubiquitin-conjugating enzyme E2 32-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT3G17000.13.8e-10763.58ubiquitin-conjugating enzyme 32 [more]
AT5G50430.12.7e-2841.04ubiquitin-conjugating enzyme 33 [more]
AT5G50430.22.7e-2841.04ubiquitin-conjugating enzyme 33 [more]
AT1G17280.13.9e-2734.67ubiquitin-conjugating enzyme 34 [more]
AT1G17280.23.9e-2734.67ubiquitin-conjugating enzyme 34 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..174
e-value: 1.0E-27
score: 108.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 244..266
NoneNo IPR availablePANTHERPTHR24068:SF303BNAC01G21910D PROTEINcoord: 7..309
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 7..309
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..173
e-value: 8.0E-47
score: 161.0
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 12..134
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 15..126
e-value: 4.3E-20
score: 71.8
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 11..166
score: 37.747028
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 13..128
e-value: 3.60716E-44
score: 145.808

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021468.1Lag0021468.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity