Lag0021431 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021431
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr7: 7638542 .. 7639274 (-)
RNA-Seq ExpressionLag0021431
SyntenyLag0021431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGAGGGACTCAAATTCATGCACGAAAATTTCGAAGATGTGGAGGAGATTGAAGTCGAATCGGATGCCCTTGATCTCATCCAATTCTTGAACAACGAAGACGAAAATATGACTGAGATCAAGAATTTAGTTGAAGCCATCAAAGGTTTGGCTCCCAGGATTGGAGTGCTGTCTTTCAAGCACTGCCCTCGAACTCAGAATGGGGTGGCTCACTCCCTTGTTCGTGAAAGTGCTTTTCACTGTTTTGATTTTTTTTTTTTTTTTTGTTGAGGAGCAGAGGCTTAACTTCCACACTAGAAGATTGTTGTAGTTATTTTTGGACCTCTGATCCGCCTCTTTGGTTATCCTCCTTAATTTTAGAGGAGATAGTTGTATCTAACAATTTTTAGTTCTGGTTATGCATTATCTTGTTTTAAAAAAAAAGACGATATTAAAAAGTGTAAAAACCAAATATATATATATATATATATATATATATATATAAACACATATGTTAAATAAATTGAATTGATTTAACGCTAAGTGCAACAATAGAATTGAATCAAGTCGAGAAAAAGAAAAATATATTGAAGATCTTGAATGAAAACCCTAATTTCAAAATATTCTTAAAATTATCTTAAAAATCACTATTTATGGTTTGACATTATAAGGAATCCAGCTAAGAACATTTGTCCTTTTTCATGAATTTTCTAATTCTAATATCTTTATAATTAAGTTAGGCAAAAGATAG

mRNA sequence

ATGTGGGAGGGACTCAAATTCATGCACGAAAATTTCGAAGATGTGGAGGAGATTGAAGTCGAATCGGATGCCCTTGATCTCATCCAATTCTTGAACAACGAAGACGAAAATATGACTGAGATCAAGAATTTAGTTGAAGCCATCAAAGGTTTGGCTCCCAGGATTGGAGTGCTGTCTTTCAAGCACTGCCCTCGAACTCAGAATGGGGTGGCTCACTCCCTTGTTCGTGAAAGCAAAAGATAG

Coding sequence (CDS)

ATGTGGGAGGGACTCAAATTCATGCACGAAAATTTCGAAGATGTGGAGGAGATTGAAGTCGAATCGGATGCCCTTGATCTCATCCAATTCTTGAACAACGAAGACGAAAATATGACTGAGATCAAGAATTTAGTTGAAGCCATCAAAGGTTTGGCTCCCAGGATTGGAGTGCTGTCTTTCAAGCACTGCCCTCGAACTCAGAATGGGGTGGCTCACTCCCTTGTTCGTGAAAGCAAAAGATAG

Protein sequence

MWEGLKFMHENFEDVEEIEVESDALDLIQFLNNEDENMTEIKNLVEAIKGLAPRIGVLSFKHCPRTQNGVAHSLVRESKR
Homology
BLAST of Lag0021431 vs. NCBI nr
Match: KAA3474504.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 56.6 bits (135), Expect = 1.1e-04
Identity = 28/64 (43.75%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 17  EIEVESDALDLIQFLNNEDENMTEIKNLVEAIKGLAPRIGVLSFKHCPRTQNGVAHSLVR 76
           EI VE D+L +I  +N+E E+ + I +L++ I+G  P+  VLSF+H PR  N VAH +  
Sbjct: 384 EICVEGDSLTVINKINSEMEDRSTISSLIKKIRGRFPKFRVLSFRHIPREANKVAHEMAC 443

Query: 77  ESKR 81
           E K+
Sbjct: 444 EGKK 447

BLAST of Lag0021431 vs. NCBI nr
Match: TYH08087.1 (hypothetical protein ES288_A08G287600v1 [Gossypium darwinii])

HSP 1 Score: 56.2 bits (134), Expect = 1.5e-04
Identity = 28/65 (43.08%), Postives = 42/65 (64.62%), Query Frame = 0

Query: 16  EEIEVESDALDLIQFLNNEDENMTEIKNLVEAIKGLAPRIGVLSFKHCPRTQNGVAHSLV 75
           ++I VE DAL +I  +N+ +EN + I N+++ IKG  P+   LSFK+ PR  N  AH +V
Sbjct: 314 QDICVEGDALTIICKVNSLEENKSNISNMIKEIKGRTPKFRRLSFKYVPREANKTAHGMV 373

Query: 76  RESKR 81
            E +R
Sbjct: 374 IEGRR 378

BLAST of Lag0021431 vs. NCBI nr
Match: TYI16890.1 (hypothetical protein ES332_A08G286100v1 [Gossypium tomentosum])

HSP 1 Score: 56.2 bits (134), Expect = 1.5e-04
Identity = 28/65 (43.08%), Postives = 42/65 (64.62%), Query Frame = 0

Query: 16  EEIEVESDALDLIQFLNNEDENMTEIKNLVEAIKGLAPRIGVLSFKHCPRTQNGVAHSLV 75
           ++I VE DAL +I  +N+ +EN + I N+++ IKG  P+   LSFK+ PR  N  AH +V
Sbjct: 357 QDICVEGDALTIICKVNSLEENKSNISNMIKEIKGRTPKFRRLSFKYVPREANKTAHGIV 416

Query: 76  RESKR 81
            E +R
Sbjct: 417 IEGRR 421

BLAST of Lag0021431 vs. NCBI nr
Match: MBA0847404.1 (hypothetical protein [Gossypium schwendimanii])

HSP 1 Score: 55.1 bits (131), Expect = 3.3e-04
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 0

Query: 16 EEIEVESDALDLIQFLNNEDENMTEIKNLVEAIKGLAPRIGVLSFKHCPRTQNGVAHSLV 75
          ++I +E DAL +I+ LN+ DE+ + I NL++ IKG       LSFKH P+  N VAH++ 
Sbjct: 35 QDICIEGDALTIIRKLNSADEDRSCISNLIKEIKGRGYNFRSLSFKHVPKEANKVAHAMA 94

Query: 76 RE 78
          +E
Sbjct: 95 KE 96

BLAST of Lag0021431 vs. NCBI nr
Match: KAA3472633.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 53.9 bits (128), Expect = 7.3e-04
Identity = 24/65 (36.92%), Postives = 41/65 (63.08%), Query Frame = 0

Query: 16  EEIEVESDALDLIQFLNNEDENMTEIKNLVEAIKGLAPRIGVLSFKHCPRTQNGVAHSLV 75
           +EI VE D+L +I+ +N+ +++ + I NL++ I+G  P+    SF+H PR  N  AH + 
Sbjct: 372 QEICVEGDSLTIIKKVNSLEDDRSNISNLIKKIRGRLPKFRATSFRHIPREANRAAHEMA 431

Query: 76  RESKR 81
           RE  +
Sbjct: 432 REGNK 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA3474504.11.1e-0443.75reverse transcriptase [Gossypium australe][more]
TYH08087.11.5e-0443.08hypothetical protein ES288_A08G287600v1 [Gossypium darwinii][more]
TYI16890.11.5e-0443.08hypothetical protein ES332_A08G286100v1 [Gossypium tomentosum][more]
MBA0847404.13.3e-0443.55hypothetical protein [Gossypium schwendimanii][more]
KAA3472633.17.3e-0436.92reverse transcriptase [Gossypium australe][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 4..77
e-value: 1.5E-11
score: 44.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..80
e-value: 2.0E-6
score: 29.8
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 15..76
e-value: 1.15277E-10
score: 51.1608

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021431.1Lag0021431.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity