Lag0021427 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021427
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr7: 7609174 .. 7610942 (+)
RNA-Seq ExpressionLag0021427
SyntenyLag0021427
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCTGTTTTCCAACAAGCCATTCTTCTTGCCTTGCTATTAGTAGTAGCATTGGTTGGTGCTGAGGCCAAGCTGCTCATCGGGCCGATCACGGCCGACCCCTTTAAAAGCTTTAAAAACATTAGACATCGAAAGGAAACGGTGTTTAATGTGCTCAGTTTTGGTGCCAAGCCTGATGGCAAGAAAGACAATACTGAGGTGACCGTTAAGAAAGACCCGTTAAACTAGGCTCAAGTTGACTTTTGTTATTGTTTAACAAGGCTTTGAATGAATTTGTTTTGCAGAGTTTTATGAAGACATGGGTTGCAGCATGTCACTCCAAGGGAGCGGCAAGAGTTGTTATTCCTAGTGGTGTTTTTTTGATCAATCAAATTGTATTTGCAGGGCCATGTACCTCTGTAGGTCCTATCGTTGTTCAAATCGATGCGACCTTAAAAGGAGGCGTCGACGTGAGCGAATACGTCTCCGATGAATGGATATTGCTTGAGAAGATCAATGGTTTGATACTCACTGGCAGAGGTACTTTGGATGGACAAGGTAGTGCAGTTTGGAAATACAACGATTGTGGTATGAATCCAACTTGCCAAAAGCTCCCTGTGGTAAGTAGTTAGACTTCATCACCTTATTGATTTTCCGGATCCTTCGTTGACAAAGGAAAAATTGTTCAATAACGTCTGTTCGTCCTCCTCTTCTTACTACTAGATATAGATTTAAAGTACTTTTGTAACTGCTAGTCACATTGAATAATTTCTCGTCGAGAAAAACAGGACCTTGAATTCAAAATTCTTTTCTCTTTCTCCTTTCTTCCTAGAACTTGAAACTGAACGGCATCACACATGGACTTGTGAAAGGCATCAGTTCCATCAACAGCAAGGGGTTTCACATGTTCATCACAAATTCTGAGAGTGTAAGGGTGAGAAAAATGCACATAACAGCGCCGGAAGACAGTCCAAACACCGATGGCATACATCTTAGCCGCTCCAACAATGTGAGGATATCAAGATCTGTCATCTCCACCGGTGACGATTGCATCTCCATCGGTCGAGGATCGACGAATGTCGCCATCAACAAAGTAACCTGCGGTCCCGGACACGGTATCAGGTAACTCCCACATCTCAAAAAACTCTCTTCAGCTATGAAATATTGCAGGGACTTAATGATCAACAGAGGCTCCTTCTACTCTGTTTGGTGTCTCTATACTATGACAGTGTCGGCAGCCTCGGAAAGTACCCAAATGAGGAGGACGTGCGCGGTATCATCGTCAGGAACTGCACGTTGACGAACACAGACAACGGACTTAGAATCAAAACATGGCCAGACTCACCCCCAAGTGCAGCCTCAGGCATACTATTCCAAGACATCAATATGATTGATGTCAAGAACCCCATCATCATAGACCAAGAGTACAGCTGCTCAACAACTTGCAATAGACCGGTTCGTTATTCTCGCTCTCGTTCTCGTTCTCGTTCTCGATTACCTCCCCTTTTCATAATATATAACTTTCTCGTTTCGATCGGTTTCGACGCACGCAGCCGTCACGAGTGCGAATCAGCGACGTTCATTTCATCAATGTGAAAGGATCATCAATCTCCCCAGTAGCAGTTCAATTGGTATGCAGCAAGAGGTTCCCTTGCCAAAACATTGAGTTGTTTAACATCAATCTGAAGTACACGGGCAACCCGAAAGCCTCCTTGCCGTTTAGCTCGCTTTGTACCAATGCAAAAGTGGGCTACAGAGGCCTTCAATTTCCTCCCCCTTGCAAATGA

mRNA sequence

ATGAAATCTGTTTTCCAACAAGCCATTCTTCTTGCCTTGCTATTAGTAGTAGCATTGGTTGGTGCTGAGGCCAAGCTGCTCATCGGGCCGATCACGGCCGACCCCTTTAAAAGCTTTAAAAACATTAGACATCGAAAGGAAACGGTGTTTAATGTGCTCAGTTTTGGTGCCAAGCCTGATGGCAAGAAAGACAATACTGAGAGTTTTATGAAGACATGGGTTGCAGCATGTCACTCCAAGGGAGCGGCAAGAGTTGTTATTCCTAGTGGTGTTTTTTTGATCAATCAAATTGTATTTGCAGGGCCATGTACCTCTGTAGGTCCTATCGTTGTTCAAATCGATGCGACCTTAAAAGGAGGCGTCGACGTGAGCGAATACGTCTCCGATGAATGGATATTGCTTGAGAAGATCAATGGTTTGATACTCACTGGCAGAGGTACTTTGGATGGACAAGGTAGTGCAGTTTGGAAATACAACGATTGTGGTATGAATCCAACTTGCCAAAAGCTCCCTGTGAACTTGAAACTGAACGGCATCACACATGGACTTGTGAAAGGCATCAGTTCCATCAACAGCAAGGGGTTTCACATGTTCATCACAAATTCTGAGAGTGTAAGGGTGAGAAAAATGCACATAACAGCGCCGGAAGACAGTCCAAACACCGATGGCATACATCTTAGCCGCTCCAACAATGTGAGGATATCAAGATCTGTCATCTCCACCGGTGACGATTGCATCTCCATCGGTCGAGGATCGACGAATGTCGCCATCAACAAAGTAACCTGCGGTCCCGGACACGGTATCAGTGTCGGCAGCCTCGGAAAGTACCCAAATGAGGAGGACGTGCGCGGTATCATCGTCAGGAACTGCACGTTGACGAACACAGACAACGGACTTAGAATCAAAACATGGCCAGACTCACCCCCAAGTGCAGCCTCAGGCATACTATTCCAAGACATCAATATGATTGATGTCAAGAACCCCATCATCATAGACCAAGAGTACAGCTGCTCAACAACTTGCAATAGACCGCCGTCACGAGTGCGAATCAGCGACGTTCATTTCATCAATGTGAAAGGATCATCAATCTCCCCAGTAGCAGTTCAATTGGTATGCAGCAAGAGGTTCCCTTGCCAAAACATTGAGTTGTTTAACATCAATCTGAAGTACACGGGCAACCCGAAAGCCTCCTTGCCGTTTAGCTCGCTTTGTACCAATGCAAAAGTGGGCTACAGAGGCCTTCAATTTCCTCCCCCTTGCAAATGA

Coding sequence (CDS)

ATGAAATCTGTTTTCCAACAAGCCATTCTTCTTGCCTTGCTATTAGTAGTAGCATTGGTTGGTGCTGAGGCCAAGCTGCTCATCGGGCCGATCACGGCCGACCCCTTTAAAAGCTTTAAAAACATTAGACATCGAAAGGAAACGGTGTTTAATGTGCTCAGTTTTGGTGCCAAGCCTGATGGCAAGAAAGACAATACTGAGAGTTTTATGAAGACATGGGTTGCAGCATGTCACTCCAAGGGAGCGGCAAGAGTTGTTATTCCTAGTGGTGTTTTTTTGATCAATCAAATTGTATTTGCAGGGCCATGTACCTCTGTAGGTCCTATCGTTGTTCAAATCGATGCGACCTTAAAAGGAGGCGTCGACGTGAGCGAATACGTCTCCGATGAATGGATATTGCTTGAGAAGATCAATGGTTTGATACTCACTGGCAGAGGTACTTTGGATGGACAAGGTAGTGCAGTTTGGAAATACAACGATTGTGGTATGAATCCAACTTGCCAAAAGCTCCCTGTGAACTTGAAACTGAACGGCATCACACATGGACTTGTGAAAGGCATCAGTTCCATCAACAGCAAGGGGTTTCACATGTTCATCACAAATTCTGAGAGTGTAAGGGTGAGAAAAATGCACATAACAGCGCCGGAAGACAGTCCAAACACCGATGGCATACATCTTAGCCGCTCCAACAATGTGAGGATATCAAGATCTGTCATCTCCACCGGTGACGATTGCATCTCCATCGGTCGAGGATCGACGAATGTCGCCATCAACAAAGTAACCTGCGGTCCCGGACACGGTATCAGTGTCGGCAGCCTCGGAAAGTACCCAAATGAGGAGGACGTGCGCGGTATCATCGTCAGGAACTGCACGTTGACGAACACAGACAACGGACTTAGAATCAAAACATGGCCAGACTCACCCCCAAGTGCAGCCTCAGGCATACTATTCCAAGACATCAATATGATTGATGTCAAGAACCCCATCATCATAGACCAAGAGTACAGCTGCTCAACAACTTGCAATAGACCGCCGTCACGAGTGCGAATCAGCGACGTTCATTTCATCAATGTGAAAGGATCATCAATCTCCCCAGTAGCAGTTCAATTGGTATGCAGCAAGAGGTTCCCTTGCCAAAACATTGAGTTGTTTAACATCAATCTGAAGTACACGGGCAACCCGAAAGCCTCCTTGCCGTTTAGCTCGCTTTGTACCAATGCAAAAGTGGGCTACAGAGGCCTTCAATTTCCTCCCCCTTGCAAATGA

Protein sequence

MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSFKNIRHRKETVFNVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATLKGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNRPPSRVRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQFPPPCK
Homology
BLAST of Lag0021427 vs. NCBI nr
Match: XP_004134494.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 731.9 bits (1888), Expect = 3.2e-207
Identity = 352/425 (82.82%), Postives = 387/425 (91.06%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           M SVF+Q ILL LLL VALV  +AKLL GPI+ DPF +    K+I+HRKETVFNVLSFGA
Sbjct: 1   MSSVFKQLILLTLLL-VALVAVDAKLLAGPISPDPFLNVNLGKDIKHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGK DN+ESFMKTWVAACHSKGAARVVIP G FLINQI FAGPC S  PI+VQIDATL
Sbjct: 61  KPDGKTDNSESFMKTWVAACHSKGAARVVIPRGTFLINQIAFAGPCASTSPIIVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGGVD+S Y SDEWIL EKINGLI+TGRG LDGQGSAVW+YNDCGMNP CQKLPVNLKLN
Sbjct: 121 KGGVDISNYFSDEWILFEKINGLIVTGRGALDGQGSAVWEYNDCGMNPNCQKLPVNLKLN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
            ITHGLVKGISS+NSKGFHMF+TN E+VR+RK+H+TAPEDSPNTDGIHLSRSNN++ISRS
Sbjct: 181 NITHGLVKGISSVNSKGFHMFVTNCENVRLRKLHLTAPEDSPNTDGIHLSRSNNIKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VI+TGDDCIS+GRGS NVAINK+TCGPGHGISVGSLGKYPNEEDVRGI+V+NCTL +TDN
Sbjct: 241 VIATGDDCISLGRGSMNVAINKITCGPGHGISVGSLGKYPNEEDVRGIVVKNCTLLSTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINM++VKNPI+IDQEYSCS T C RPPSRV+ISDVH+I
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMVNVKNPILIDQEYSCSKTNCQRPPSRVKISDVHYI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGSSISPVAVQL CSK+FPCQNIE+FNINLKY+G P   LPFS+ C+NAKVGYRGLQF
Sbjct: 361 NVKGSSISPVAVQLKCSKQFPCQNIEMFNINLKYSGPPNKELPFSAKCSNAKVGYRGLQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 424

BLAST of Lag0021427 vs. NCBI nr
Match: XP_008438909.1 (PREDICTED: exopolygalacturonase-like [Cucumis melo] >KAA0049517.1 exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK16197.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 730.7 bits (1885), Expect = 7.1e-207
Identity = 354/425 (83.29%), Postives = 385/425 (90.59%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           M S F+Q ILL LLL VALV  +AKLL GPI  DPF++    K+I+HRKETVFNVLSFGA
Sbjct: 1   MSSFFKQVILLTLLL-VALVAVDAKLLPGPILPDPFRNVNLGKDIKHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGK DNTESFMKTWVAACHSKGAARVVIPSG FLINQ+ FAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKTDNTESFMKTWVAACHSKGAARVVIPSGTFLINQVAFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGG+D+S Y SDEWIL EKINGLI+TGRGTLDGQGSAVW+YNDCGMNP CQKLPVNLKL+
Sbjct: 121 KGGIDISNYFSDEWILFEKINGLIVTGRGTLDGQGSAVWEYNDCGMNPNCQKLPVNLKLS 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GITHGLVKGISS+NSKGFHMFITN E+VR+RK+HITAPE SPNTDGIH+SRSNNV+ISRS
Sbjct: 181 GITHGLVKGISSVNSKGFHMFITNCENVRLRKLHITAPETSPNTDGIHVSRSNNVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VI+TGDDCIS+GRGS N+AINK+TCGPGHGISVGSLGKY NEEDVRGIIV+NCTL  TDN
Sbjct: 241 VIATGDDCISLGRGSMNIAINKITCGPGHGISVGSLGKYQNEEDVRGIIVKNCTLVKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C +PPSRV+ISDVH+I
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQKPPSRVKISDVHYI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGSSISPVAVQL CSK+FPCQNIE+FNINLKYTG P   LPF + C+NAKVGYRGLQF
Sbjct: 361 NVKGSSISPVAVQLKCSKQFPCQNIEMFNINLKYTGPPNKELPFIAQCSNAKVGYRGLQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 424

BLAST of Lag0021427 vs. NCBI nr
Match: XP_038877778.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 728.8 bits (1880), Expect = 2.7e-206
Identity = 351/425 (82.59%), Postives = 385/425 (90.59%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           MKS F+Q + L LLL VALV  EAK L GPI  +P ++    K+IRHRKE VFNVLSFGA
Sbjct: 1   MKSAFKQVVPLTLLL-VALVAVEAKPLAGPILPNPLQNVNLGKDIRHRKEAVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGK DNT+SFMKTW+AACHSKGAARVVIPSG FLINQ+ FAGPCTS+ PI+VQIDA L
Sbjct: 61  KPDGKTDNTQSFMKTWIAACHSKGAARVVIPSGTFLINQVAFAGPCTSISPIIVQIDAML 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGG DVS YVSDEWIL EKINGLI+TGRGTLDGQG AVW+YNDCGMNP CQKLPVNLKLN
Sbjct: 121 KGGTDVSNYVSDEWILFEKINGLIVTGRGTLDGQGGAVWQYNDCGMNPNCQKLPVNLKLN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GITHGLVKGISSINSKGFHMFITN E++R+RK+HITAP DSPNTDGIH SRSNNV+ISRS
Sbjct: 181 GITHGLVKGISSINSKGFHMFITNCENLRLRKLHITAPVDSPNTDGIHTSRSNNVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VI+TGDDCIS+GRGS N+AINK+TCGPGHGIS+GSLGKYPNEEDVRGIIV+NCTLTNTDN
Sbjct: 241 VIATGDDCISLGRGSMNIAINKITCGPGHGISIGSLGKYPNEEDVRGIIVKNCTLTNTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMI+VKNPIIIDQEYSCS T C +PPSRV+ISDVH+I
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMINVKNPIIIDQEYSCSKTNCQKPPSRVKISDVHYI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGSSISPVAV+L CSK+FPCQNIE+FNINLKY+G P   LPFS+ CTNAKVGYRG+QF
Sbjct: 361 NVKGSSISPVAVRLKCSKQFPCQNIEMFNINLKYSGPPNKELPFSAACTNAKVGYRGIQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 424

BLAST of Lag0021427 vs. NCBI nr
Match: XP_022980001.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 716.8 bits (1849), Expect = 1.1e-202
Identity = 348/425 (81.88%), Postives = 377/425 (88.71%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           MKS F+QA LL  LL+V LV   AK  +G    D F+     K+IRHRKETVFNVLSFGA
Sbjct: 1   MKSAFKQATLLTWLLLVELVAVNAKFFVGTNPPDLFQKINFGKDIRHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGKKDNT+SFMKTWVAACHSKGAARVVIP G FLINQIVFAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKKDNTQSFMKTWVAACHSKGAARVVIPGGTFLINQIVFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGG+D+S YVS+EWIL EKINGLI+TGRGTLDGQG AVWKYNDCGMNPTCQKLPVNLK N
Sbjct: 121 KGGIDLSNYVSNEWILFEKINGLIITGRGTLDGQGDAVWKYNDCGMNPTCQKLPVNLKFN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GIT+  +KGISSINSKGFHMFITNSE+VR++K+HITAP+DSPNTDGIHLSRSN V+ISRS
Sbjct: 181 GITNVFMKGISSINSKGFHMFITNSENVRLKKLHITAPDDSPNTDGIHLSRSNKVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VISTGDDCIS+GRGS NV INK+TCGPGHGISVGSLGKY NEEDV G++++NCTL  TDN
Sbjct: 241 VISTGDDCISLGRGSMNVTINKITCGPGHGISVGSLGKYDNEEDVLGVVIKNCTLIKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C RPPSRV+ISDVHFI
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQRPPSRVKISDVHFI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGS+IS VAV L CSKRFPCQNIELFNINLKY+G PK SLPF S CTNAKVGYRG+QF
Sbjct: 361 NVKGSTISSVAVHLKCSKRFPCQNIELFNINLKYSGPPKKSLPFVSTCTNAKVGYRGVQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 425

BLAST of Lag0021427 vs. NCBI nr
Match: XP_022955822.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 715.7 bits (1846), Expect = 2.4e-202
Identity = 348/425 (81.88%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           MKS F+QA LLA LL+VALV   AK  +G    D F+     K+IRHRKETVFNVLSFGA
Sbjct: 1   MKSAFKQATLLAWLLLVALVAVNAKFFVGTNPPDLFQKINFGKDIRHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGKKDNT+SFMKTWVAACHSKGAARVVIP G FLI+QIVFAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKKDNTQSFMKTWVAACHSKGAARVVIPGGTFLISQIVFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KG +D+S YVS+EWIL EKINGLI+TGRGTLDGQG AVWKYNDCGMNPTCQKLPVNLK N
Sbjct: 121 KGDIDLSNYVSNEWILFEKINGLIITGRGTLDGQGDAVWKYNDCGMNPTCQKLPVNLKFN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GIT+  +KGISSINSKGFHMFITNSE+VR++K+HITAP+DSPNTDGIHLSRSN V+ISRS
Sbjct: 181 GITNVFMKGISSINSKGFHMFITNSENVRLKKLHITAPDDSPNTDGIHLSRSNKVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VISTGDDCIS+GRGS NV INK+TCGPGHGISVGSLGKY NEEDV G++++NCTL  TDN
Sbjct: 241 VISTGDDCISLGRGSMNVTINKITCGPGHGISVGSLGKYENEEDVLGVVIKNCTLIKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C RPPSRV+ISDVHFI
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQRPPSRVKISDVHFI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGS+IS VAV L CSKRFPCQNIELFNINLKY+G PK SLPF S CTNAKVGYRG+QF
Sbjct: 361 NVKGSTISSVAVHLKCSKRFPCQNIELFNINLKYSGPPKKSLPFVSTCTNAKVGYRGVQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 425

BLAST of Lag0021427 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 2.1e-97
Identity = 177/377 (46.95%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 48  TVFNVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVG 107
           TV+++  FGA  DG  +  ++F+ TW+  C S   A +++P G FL   ++FAGPC S  
Sbjct: 50  TVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKS-- 109

Query: 108 PIVVQIDATLKGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTC 167
            + V +  T+      S Y + EW L E+++ L+LTG GT  G+G AVWK + CG    C
Sbjct: 110 KVTVNVIGTII--ATTSGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQC 169

Query: 168 QKLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLS 227
              P +LK   + +  + GISS+N+K FHMF+  +E+V ++ + +TAP +SPNTDGIHLS
Sbjct: 170 NLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLS 229

Query: 228 RSNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIV 287
            ++NV I  S I+TGDDC+S+GRGS NV + +V CGPGHG+SVGSLGKY NEEDV GI V
Sbjct: 230 NADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHV 289

Query: 288 RNCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNRPPSR 347
            NCT+  TDNGLRIKTW  S PS A  I F++I M  VKNPIIIDQ Y          S+
Sbjct: 290 NNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYG----SRGGDSQ 349

Query: 348 VRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSS----- 407
           V ISD+ F N++G++I+   VQ++CSK  PCQ + + ++NL Y G        SS     
Sbjct: 350 VAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVG 409

Query: 408 -LCTNAKVGYRG-LQFP 418
            LC NA V + G L FP
Sbjct: 410 ALCDNANVIFGGKLSFP 418

BLAST of Lag0021427 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 6.2e-97
Identity = 171/371 (46.09%), Postives = 245/371 (66.04%), Query Frame = 0

Query: 48  TVFNVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVG 107
           +VFNV  +GAK  G  D +++ MK W AAC S+G + V+IP G + + ++   GPC    
Sbjct: 8   SVFNVNDYGAK--GAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKG-S 67

Query: 108 PIVVQIDATLKGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTC 167
            I  QID  +K   D S++ SD W+   +I+GL ++G GTLDGQG   W  N+C  NP C
Sbjct: 68  KIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNC 127

Query: 168 QKLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLS 227
           +   +NL+ + + H +V+ I+S+NSK FH+ +   E +  + + +TAP  S NTDGIH+ 
Sbjct: 128 KHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVG 187

Query: 228 RSNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIV 287
            S  V I+ + I+TGDDCISIG GS NV I +V CGPGHGIS+GSLG+Y NE++VRGI V
Sbjct: 188 ISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITV 247

Query: 288 RNCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNR-PPS 347
           + CT + T NG+R+KTWP+SPP AA+ + FQD+ M +V+NP+I+DQEY     C+R  PS
Sbjct: 248 KGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPS 307

Query: 348 RVRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTN 407
           R+++S+++F N++G+S   VAV + CS   PC N+++  INL Y G   A  P +S C+N
Sbjct: 308 RIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRG---AGGPATSTCSN 367

Query: 408 AKVGYRGLQFP 418
            K  + G Q P
Sbjct: 368 VKPTFSGKQVP 372

BLAST of Lag0021427 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 4.0e-88
Identity = 164/373 (43.97%), Postives = 238/373 (63.81%), Query Frame = 0

Query: 49  VFNVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGP 108
           VF++  +GA  +   D +E+ +  +  AC S   + +VIP G F +NQ+   GPC S  P
Sbjct: 26  VFDITKYGA--NSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKS--P 85

Query: 109 IVVQIDATLKGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQ 168
           + +QI ATLK   D S+    EW+ + K++   ++G G LDGQ +A W   +C  +  C 
Sbjct: 86  LELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAW---ECKQSKKCN 145

Query: 169 KLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSR 228
           KLP NL  N +T+  +K I++++SK FH+ +   +++   + +++AP +SPNTDGIH+SR
Sbjct: 146 KLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSR 205

Query: 229 SNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVR 288
           S++V I+ S  STGDDCIS+G  +  + I +VTCGPGHGISVGSLG  P+E+ V G+ VR
Sbjct: 206 SSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVR 265

Query: 289 NCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNRP-PSR 348
           NCT TNTDNG+RIKTWP S P   + + F+DI + +V NP++IDQ Y     CN+  PS+
Sbjct: 266 NCTFTNTDNGVRIKTWPASHPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQ 325

Query: 349 VRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNA 408
           V+IS V F N+KG+S +  AV L+ SK  PC+ IE+ +I++ Y+G      P  S C N 
Sbjct: 326 VKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEG---PAKSSCENI 385

Query: 409 KVGYRGLQFPPPC 421
           K   +G Q PP C
Sbjct: 386 KPSLKGKQNPPVC 388

BLAST of Lag0021427 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.3e-86
Identity = 157/370 (42.43%), Postives = 230/370 (62.16%), Query Frame = 0

Query: 52  VLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVV 111
           V  FGAK DGK D ++ F+  W  AC S   + VVIP G +L++++   GPC +  PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKA--PIEI 91

Query: 112 QIDATLKGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLP 171
            +  T++   D S +    W+    +    + G G  DGQGS  ++ N C       KLP
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 172 VNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNN 231
           VN++ + +T+ L++ I+S +SK FH+ +   +++ + ++ I AP++SPNTDGIH+ +S  
Sbjct: 152 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 232 VRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCT 291
           V I  S I TGDDCISIG G+ N+ I ++TCGPGHGIS+GSLGK+ NEE V GI + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 292 LTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNR-PPSRVRI 351
           +TNT NG RIKTWP     A S I F+DI M +V +PI+IDQ+Y     C +   S+V++
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKL 331

Query: 352 SDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVG 411
           S++ F N++G+S  P A++ +CS   PCQN+EL +I++K+ G    + P +S C N K  
Sbjct: 332 SNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNG----AEPATSQCLNVKPI 391

Query: 412 YRGLQFPPPC 421
             G   P PC
Sbjct: 392 TSGKLNPIPC 395

BLAST of Lag0021427 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 321.2 bits (822), Expect = 1.7e-86
Identity = 163/373 (43.70%), Postives = 223/373 (59.79%), Query Frame = 0

Query: 51  NVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIV 110
           +V + GAK D K D++ +F   W  AC     + + +P G +++  + F GPC   GP+ 
Sbjct: 71  DVKASGAKGDSKTDDSAAFAAAWKEAC--AAGSTITVPKGEYMVESLEFKGPCK--GPVT 130

Query: 111 VQIDATLKGGVDVSEYVSDE-WILLEKINGLILTG-RGTLDGQGSAVWKYNDCGMNPTCQ 170
           ++++   K    V        WI  E I    L G +   DGQGS  WK NDC     C 
Sbjct: 131 LELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCN 190

Query: 171 KLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSR 230
            LP+N++  G+T+  +  I+S NSK FHM I N +++ +  + I AP +S NTDGIH+ R
Sbjct: 191 SLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGR 250

Query: 231 SNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVR 290
           SN V +  + I TGDDC+SIG G+ N+ +  V CGPGHGIS+GSLG+YPNE+ V+G+ VR
Sbjct: 251 SNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVR 310

Query: 291 NCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNR-PPSR 350
            C + NTDNG+RIKTWP SPP  AS ILF+DI M +V  P++IDQEY     C    PS+
Sbjct: 311 KCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQ 370

Query: 351 VRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNA 410
           V++SDV    +KG+S + VAV+L+CSK  PC NI L +INL + G      P  S C+N 
Sbjct: 371 VKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG---PAVSACSNI 430

Query: 411 KVGYRGLQFPPPC 421
           K    G   P  C
Sbjct: 431 KPILSGKLVPAAC 436

BLAST of Lag0021427 vs. ExPASy TrEMBL
Match: A0A0A0LAM9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164550 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.5e-207
Identity = 352/425 (82.82%), Postives = 387/425 (91.06%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           M SVF+Q ILL LLL VALV  +AKLL GPI+ DPF +    K+I+HRKETVFNVLSFGA
Sbjct: 1   MSSVFKQLILLTLLL-VALVAVDAKLLAGPISPDPFLNVNLGKDIKHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGK DN+ESFMKTWVAACHSKGAARVVIP G FLINQI FAGPC S  PI+VQIDATL
Sbjct: 61  KPDGKTDNSESFMKTWVAACHSKGAARVVIPRGTFLINQIAFAGPCASTSPIIVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGGVD+S Y SDEWIL EKINGLI+TGRG LDGQGSAVW+YNDCGMNP CQKLPVNLKLN
Sbjct: 121 KGGVDISNYFSDEWILFEKINGLIVTGRGALDGQGSAVWEYNDCGMNPNCQKLPVNLKLN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
            ITHGLVKGISS+NSKGFHMF+TN E+VR+RK+H+TAPEDSPNTDGIHLSRSNN++ISRS
Sbjct: 181 NITHGLVKGISSVNSKGFHMFVTNCENVRLRKLHLTAPEDSPNTDGIHLSRSNNIKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VI+TGDDCIS+GRGS NVAINK+TCGPGHGISVGSLGKYPNEEDVRGI+V+NCTL +TDN
Sbjct: 241 VIATGDDCISLGRGSMNVAINKITCGPGHGISVGSLGKYPNEEDVRGIVVKNCTLLSTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINM++VKNPI+IDQEYSCS T C RPPSRV+ISDVH+I
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMVNVKNPILIDQEYSCSKTNCQRPPSRVKISDVHYI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGSSISPVAVQL CSK+FPCQNIE+FNINLKY+G P   LPFS+ C+NAKVGYRGLQF
Sbjct: 361 NVKGSSISPVAVQLKCSKQFPCQNIEMFNINLKYSGPPNKELPFSAKCSNAKVGYRGLQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 424

BLAST of Lag0021427 vs. ExPASy TrEMBL
Match: A0A5D3CWB7 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001250 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.4e-207
Identity = 354/425 (83.29%), Postives = 385/425 (90.59%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           M S F+Q ILL LLL VALV  +AKLL GPI  DPF++    K+I+HRKETVFNVLSFGA
Sbjct: 1   MSSFFKQVILLTLLL-VALVAVDAKLLPGPILPDPFRNVNLGKDIKHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGK DNTESFMKTWVAACHSKGAARVVIPSG FLINQ+ FAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKTDNTESFMKTWVAACHSKGAARVVIPSGTFLINQVAFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGG+D+S Y SDEWIL EKINGLI+TGRGTLDGQGSAVW+YNDCGMNP CQKLPVNLKL+
Sbjct: 121 KGGIDISNYFSDEWILFEKINGLIVTGRGTLDGQGSAVWEYNDCGMNPNCQKLPVNLKLS 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GITHGLVKGISS+NSKGFHMFITN E+VR+RK+HITAPE SPNTDGIH+SRSNNV+ISRS
Sbjct: 181 GITHGLVKGISSVNSKGFHMFITNCENVRLRKLHITAPETSPNTDGIHVSRSNNVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VI+TGDDCIS+GRGS N+AINK+TCGPGHGISVGSLGKY NEEDVRGIIV+NCTL  TDN
Sbjct: 241 VIATGDDCISLGRGSMNIAINKITCGPGHGISVGSLGKYQNEEDVRGIIVKNCTLVKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C +PPSRV+ISDVH+I
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQKPPSRVKISDVHYI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGSSISPVAVQL CSK+FPCQNIE+FNINLKYTG P   LPF + C+NAKVGYRGLQF
Sbjct: 361 NVKGSSISPVAVQLKCSKQFPCQNIEMFNINLKYTGPPNKELPFIAQCSNAKVGYRGLQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 424

BLAST of Lag0021427 vs. ExPASy TrEMBL
Match: A0A1S3AXH3 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103483868 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.4e-207
Identity = 354/425 (83.29%), Postives = 385/425 (90.59%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           M S F+Q ILL LLL VALV  +AKLL GPI  DPF++    K+I+HRKETVFNVLSFGA
Sbjct: 1   MSSFFKQVILLTLLL-VALVAVDAKLLPGPILPDPFRNVNLGKDIKHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGK DNTESFMKTWVAACHSKGAARVVIPSG FLINQ+ FAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKTDNTESFMKTWVAACHSKGAARVVIPSGTFLINQVAFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGG+D+S Y SDEWIL EKINGLI+TGRGTLDGQGSAVW+YNDCGMNP CQKLPVNLKL+
Sbjct: 121 KGGIDISNYFSDEWILFEKINGLIVTGRGTLDGQGSAVWEYNDCGMNPNCQKLPVNLKLS 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GITHGLVKGISS+NSKGFHMFITN E+VR+RK+HITAPE SPNTDGIH+SRSNNV+ISRS
Sbjct: 181 GITHGLVKGISSVNSKGFHMFITNCENVRLRKLHITAPETSPNTDGIHVSRSNNVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VI+TGDDCIS+GRGS N+AINK+TCGPGHGISVGSLGKY NEEDVRGIIV+NCTL  TDN
Sbjct: 241 VIATGDDCISLGRGSMNIAINKITCGPGHGISVGSLGKYQNEEDVRGIIVKNCTLVKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C +PPSRV+ISDVH+I
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQKPPSRVKISDVHYI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGSSISPVAVQL CSK+FPCQNIE+FNINLKYTG P   LPF + C+NAKVGYRGLQF
Sbjct: 361 NVKGSSISPVAVQLKCSKQFPCQNIEMFNINLKYTGPPNKELPFIAQCSNAKVGYRGLQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 424

BLAST of Lag0021427 vs. ExPASy TrEMBL
Match: A0A6J1ISC3 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111479527 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 5.1e-203
Identity = 348/425 (81.88%), Postives = 377/425 (88.71%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           MKS F+QA LL  LL+V LV   AK  +G    D F+     K+IRHRKETVFNVLSFGA
Sbjct: 1   MKSAFKQATLLTWLLLVELVAVNAKFFVGTNPPDLFQKINFGKDIRHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGKKDNT+SFMKTWVAACHSKGAARVVIP G FLINQIVFAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKKDNTQSFMKTWVAACHSKGAARVVIPGGTFLINQIVFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KGG+D+S YVS+EWIL EKINGLI+TGRGTLDGQG AVWKYNDCGMNPTCQKLPVNLK N
Sbjct: 121 KGGIDLSNYVSNEWILFEKINGLIITGRGTLDGQGDAVWKYNDCGMNPTCQKLPVNLKFN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GIT+  +KGISSINSKGFHMFITNSE+VR++K+HITAP+DSPNTDGIHLSRSN V+ISRS
Sbjct: 181 GITNVFMKGISSINSKGFHMFITNSENVRLKKLHITAPDDSPNTDGIHLSRSNKVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VISTGDDCIS+GRGS NV INK+TCGPGHGISVGSLGKY NEEDV G++++NCTL  TDN
Sbjct: 241 VISTGDDCISLGRGSMNVTINKITCGPGHGISVGSLGKYDNEEDVLGVVIKNCTLIKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C RPPSRV+ISDVHFI
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQRPPSRVKISDVHFI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGS+IS VAV L CSKRFPCQNIELFNINLKY+G PK SLPF S CTNAKVGYRG+QF
Sbjct: 361 NVKGSTISSVAVHLKCSKRFPCQNIELFNINLKYSGPPKKSLPFVSTCTNAKVGYRGVQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 425

BLAST of Lag0021427 vs. ExPASy TrEMBL
Match: A0A6J1GUN7 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457697 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.1e-202
Identity = 348/425 (81.88%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MKSVFQQAILLALLLVVALVGAEAKLLIGPITADPFKSF---KNIRHRKETVFNVLSFGA 60
           MKS F+QA LLA LL+VALV   AK  +G    D F+     K+IRHRKETVFNVLSFGA
Sbjct: 1   MKSAFKQATLLAWLLLVALVAVNAKFFVGTNPPDLFQKINFGKDIRHRKETVFNVLSFGA 60

Query: 61  KPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATL 120
           KPDGKKDNT+SFMKTWVAACHSKGAARVVIP G FLI+QIVFAGPC S  PIVVQIDATL
Sbjct: 61  KPDGKKDNTQSFMKTWVAACHSKGAARVVIPGGTFLISQIVFAGPCASTSPIVVQIDATL 120

Query: 121 KGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLN 180
           KG +D+S YVS+EWIL EKINGLI+TGRGTLDGQG AVWKYNDCGMNPTCQKLPVNLK N
Sbjct: 121 KGDIDLSNYVSNEWILFEKINGLIITGRGTLDGQGDAVWKYNDCGMNPTCQKLPVNLKFN 180

Query: 181 GITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRS 240
           GIT+  +KGISSINSKGFHMFITNSE+VR++K+HITAP+DSPNTDGIHLSRSN V+ISRS
Sbjct: 181 GITNVFMKGISSINSKGFHMFITNSENVRLKKLHITAPDDSPNTDGIHLSRSNKVKISRS 240

Query: 241 VISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDN 300
           VISTGDDCIS+GRGS NV INK+TCGPGHGISVGSLGKY NEEDV G++++NCTL  TDN
Sbjct: 241 VISTGDDCISLGRGSMNVTINKITCGPGHGISVGSLGKYENEEDVLGVVIKNCTLIKTDN 300

Query: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCS-TTCNRPPSRVRISDVHFI 360
           GLRIKTWPDSPPSAASGILFQDINMIDVKNPI+IDQEYSCS T C RPPSRV+ISDVHFI
Sbjct: 301 GLRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQRPPSRVKISDVHFI 360

Query: 361 NVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQF 420
           NVKGS+IS VAV L CSKRFPCQNIELFNINLKY+G PK SLPF S CTNAKVGYRG+QF
Sbjct: 361 NVKGSTISSVAVHLKCSKRFPCQNIELFNINLKYSGPPKKSLPFVSTCTNAKVGYRGVQF 420

Query: 421 PPPCK 422
           PPPCK
Sbjct: 421 PPPCK 425

BLAST of Lag0021427 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 357.5 bits (916), Expect = 1.5e-98
Identity = 177/377 (46.95%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 48  TVFNVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVG 107
           TV+++  FGA  DG  +  ++F+ TW+  C S   A +++P G FL   ++FAGPC S  
Sbjct: 50  TVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKS-- 109

Query: 108 PIVVQIDATLKGGVDVSEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTC 167
            + V +  T+      S Y + EW L E+++ L+LTG GT  G+G AVWK + CG    C
Sbjct: 110 KVTVNVIGTII--ATTSGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQC 169

Query: 168 QKLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLS 227
              P +LK   + +  + GISS+N+K FHMF+  +E+V ++ + +TAP +SPNTDGIHLS
Sbjct: 170 NLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLS 229

Query: 228 RSNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIV 287
            ++NV I  S I+TGDDC+S+GRGS NV + +V CGPGHG+SVGSLGKY NEEDV GI V
Sbjct: 230 NADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHV 289

Query: 288 RNCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNRPPSR 347
            NCT+  TDNGLRIKTW  S PS A  I F++I M  VKNPIIIDQ Y          S+
Sbjct: 290 NNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYG----SRGGDSQ 349

Query: 348 VRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSS----- 407
           V ISD+ F N++G++I+   VQ++CSK  PCQ + + ++NL Y G        SS     
Sbjct: 350 VAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVG 409

Query: 408 -LCTNAKVGYRG-LQFP 418
            LC NA V + G L FP
Sbjct: 410 ALCDNANVIFGGKLSFP 418

BLAST of Lag0021427 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 6.4e-97
Identity = 175/389 (44.99%), Postives = 258/389 (66.32%), Query Frame = 0

Query: 46  KETVF--NVLSFGAKPDGKKDNTESFMKTWVAACHSKGAA-RVVIPSGVFLINQIVFAGP 105
           K +VF  +V SFGA+ +  +D+T++F+  W  AC S  ++  ++IP G F +  + F+GP
Sbjct: 29  KSSVFLVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGP 88

Query: 106 CTSVGPIVVQIDATLKGGVDVSEYVS-DEWILLEKINGLILTGRGTLDGQGSAVWKYNDC 165
           CT+V  + V++    K   D+S+Y S   WI    INGL LTG GT DGQG+  W +N+C
Sbjct: 89  CTNVSNLTVRV----KASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNC 148

Query: 166 GMNPTCQKLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNT 225
             +  C+ LP +LK  G+   +V+ ISS+NSK FH+ +      +  +++ITAP DSPNT
Sbjct: 149 TSDSNCKLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNT 208

Query: 226 DGIHLSRSNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEED 285
           DGIH+ RS+NV  SRS I+TGDDC+SIG+G++ + I  + CGPGHGISVGSLG+YPNE+D
Sbjct: 209 DGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKD 268

Query: 286 VRGIIVRNCTLTNTDNGLRIKTWPDSPP-SAASGILFQDINMIDVKNPIIIDQEYSCSTT 345
           V G++V++C ++ T NG+RIKTW +SP  SAA+ + F++I M +V NPIIIDQ Y   ++
Sbjct: 269 VNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSS 328

Query: 346 C-NRPPSRVRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNP----- 405
           C +  PS+V +S+++F N++G+S S VAVQL CS+  PC+ + L N++L  + +      
Sbjct: 329 CISNVPSKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQ 388

Query: 406 ---KASLPFSSLCTNAKVGYRGLQFPPPC 421
              + +   SS C N +  Y G Q PPPC
Sbjct: 389 SSNRGNEAVSSSCRNVRANYIGTQIPPPC 413

BLAST of Lag0021427 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 3.8e-89
Identity = 165/373 (44.24%), Postives = 225/373 (60.32%), Query Frame = 0

Query: 51  NVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIV 110
           +V + GAK DGK D++ +F   W  AC     + + +P G +L+  + F GPC   GP+ 
Sbjct: 72  DVKASGAKGDGKTDDSAAFAAAWKEAC--AAGSTITVPKGEYLVESLEFKGPCK--GPVT 131

Query: 111 VQIDATLKGGVDVSEYVSDE-WILLEKINGLILTG-RGTLDGQGSAVWKYNDCGMNPTCQ 170
           ++++   K    V        WI  E +    L G +   DGQGS  WK NDC     C 
Sbjct: 132 LELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCN 191

Query: 171 KLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSR 230
            LP+N++  G+T+  +  I+S NSK FHM I N +++ +  + I AP +S NTDGIH+ R
Sbjct: 192 SLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGR 251

Query: 231 SNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVR 290
           SN V +  + I TGDDC+SIG G+ N+ +  V CGPGHGIS+GSLG+YPNE+ V+G+ VR
Sbjct: 252 SNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVR 311

Query: 291 NCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNR-PPSR 350
            C + NTDNG+RIKTWP SPP  AS ILF+DI M +V  P++IDQEY     C    PS+
Sbjct: 312 KCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSK 371

Query: 351 VRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNA 410
           V++SDV   N+KG+S + VAV+L+CSK  PC NI L +INL + G      P  S C+N 
Sbjct: 372 VKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG---PAVSACSNI 431

Query: 411 KVGYRGLQFPPPC 421
           K    G   P  C
Sbjct: 432 KPILSGKLVPAAC 437

BLAST of Lag0021427 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.4e-88
Identity = 162/359 (45.13%), Postives = 236/359 (65.74%), Query Frame = 0

Query: 64  DNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIVVQIDATLKGGVDV 123
           D T++ +K + +AC S   ++VVIP G F + +I   GPC +  PI V +  T+K   + 
Sbjct: 34  DITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKA--PIEVTLQGTVKADGNA 93

Query: 124 SEYVSDEWILLEKINGLILTGRGTLDGQGSAVWKYNDCGMNPTCQKLPVNLKLNGITHGL 183
            +   ++W++   I+G  L G G  DG+G+A W+ N+C     C+KLP++++ + I +  
Sbjct: 94  IQ-GKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSE 153

Query: 184 VKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSRSNNVRISRSVISTGD 243
           ++ ISSI++K FH+ +  ++++ +  + I APEDSPNTDGIHL RS+ V+I  S ISTGD
Sbjct: 154 IRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGD 213

Query: 244 DCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVRNCTLTNTDNGLRIKT 303
           DCIS+G G  N+ + KVTCGPGHGISVGSLG+Y +E+DV GI V NCTL  TDNGLRIKT
Sbjct: 214 DCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKT 273

Query: 304 WPDSPPS-AASGILFQDINMIDVKNPIIIDQEYSCSTTCNR-PPSRVRISDVHFINVKGS 363
           WP +  S  AS I F+DI + DV NPI+IDQEY     CN+   S +++ ++ F N++G+
Sbjct: 274 WPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGT 333

Query: 364 SISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNAKVGYRGLQFPPPC 421
           S +  AV+L+CSK +PCQN+E+ +I++KY G   A  P +  C+N      G Q P  C
Sbjct: 334 SGNKDAVKLLCSKGYPCQNVEIGDIDIKYNG---ADGPATFHCSNVSPKILGSQSPKAC 386

BLAST of Lag0021427 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-87
Identity = 163/373 (43.70%), Postives = 223/373 (59.79%), Query Frame = 0

Query: 51  NVLSFGAKPDGKKDNTESFMKTWVAACHSKGAARVVIPSGVFLINQIVFAGPCTSVGPIV 110
           +V + GAK D K D++ +F   W  AC     + + +P G +++  + F GPC   GP+ 
Sbjct: 71  DVKASGAKGDSKTDDSAAFAAAWKEAC--AAGSTITVPKGEYMVESLEFKGPCK--GPVT 130

Query: 111 VQIDATLKGGVDVSEYVSDE-WILLEKINGLILTG-RGTLDGQGSAVWKYNDCGMNPTCQ 170
           ++++   K    V        WI  E I    L G +   DGQGS  WK NDC     C 
Sbjct: 131 LELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCN 190

Query: 171 KLPVNLKLNGITHGLVKGISSINSKGFHMFITNSESVRVRKMHITAPEDSPNTDGIHLSR 230
            LP+N++  G+T+  +  I+S NSK FHM I N +++ +  + I AP +S NTDGIH+ R
Sbjct: 191 SLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGR 250

Query: 231 SNNVRISRSVISTGDDCISIGRGSTNVAINKVTCGPGHGISVGSLGKYPNEEDVRGIIVR 290
           SN V +  + I TGDDC+SIG G+ N+ +  V CGPGHGIS+GSLG+YPNE+ V+G+ VR
Sbjct: 251 SNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVR 310

Query: 291 NCTLTNTDNGLRIKTWPDSPPSAASGILFQDINMIDVKNPIIIDQEYSCSTTCNR-PPSR 350
            C + NTDNG+RIKTWP SPP  AS ILF+DI M +V  P++IDQEY     C    PS+
Sbjct: 311 KCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQ 370

Query: 351 VRISDVHFINVKGSSISPVAVQLVCSKRFPCQNIELFNINLKYTGNPKASLPFSSLCTNA 410
           V++SDV    +KG+S + VAV+L+CSK  PC NI L +INL + G      P  S C+N 
Sbjct: 371 VKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG---PAVSACSNI 430

Query: 411 KVGYRGLQFPPPC 421
           K    G   P  C
Sbjct: 431 KPILSGKLVPAAC 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004134494.13.2e-20782.82exopolygalacturonase [Cucumis sativus][more]
XP_008438909.17.1e-20783.29PREDICTED: exopolygalacturonase-like [Cucumis melo] >KAA0049517.1 exopolygalactu... [more]
XP_038877778.12.7e-20682.59exopolygalacturonase-like [Benincasa hispida][more]
XP_022980001.11.1e-20281.88exopolygalacturonase-like [Cucurbita maxima][more]
XP_022955822.12.4e-20281.88exopolygalacturonase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P490622.1e-9746.95Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ... [more]
Q6H9K06.2e-9746.09Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q059674.0e-8843.97Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Q397861.3e-8642.43Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
P490631.7e-8643.70Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LAM91.5e-20782.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164550 PE=3 SV=1[more]
A0A5D3CWB73.4e-20783.29Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3AXH33.4e-20783.29exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103483868 PE=3 SV=1[more]
A0A6J1ISC35.1e-20381.88exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111479527 PE=3 SV=1[more]
A0A6J1GUN71.1e-20281.88exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457697 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G02790.11.5e-9846.95polygalacturonase 4 [more]
AT4G18180.16.4e-9744.99Pectin lyase-like superfamily protein [more]
AT3G14040.13.8e-8944.24Pectin lyase-like superfamily protein [more]
AT3G07820.11.4e-8845.13Pectin lyase-like superfamily protein [more]
AT3G07850.11.2e-8743.70Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 252..272
e-value: 9700.0
score: 0.2
coord: 229..250
e-value: 150.0
score: 11.2
coord: 312..333
e-value: 7400.0
score: 1.0
coord: 282..303
e-value: 2.2
score: 17.3
coord: 202..228
e-value: 2100.0
score: 5.0
coord: 345..367
e-value: 2100.0
score: 4.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 77..407
e-value: 3.9E-92
score: 308.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 259..272
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 36..415
e-value: 5.4E-111
score: 373.5
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 12..420
NoneNo IPR availablePANTHERPTHR31375:SF117EXOPOLYGALACTURONASE-LIKEcoord: 12..420
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 46..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021427.1Lag0021427.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity