Lag0021317 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0021317 vs. NCBI nr
Match: XP_008438729.1 (PREDICTED: endo-1,3;1,4-beta-D-glucanase-like isoform X1 [Cucumis melo] >KAA0049403.1 endo-1,3 [Cucumis melo var. makuwa] >TYK16081.1 endo-1,3 [Cucumis melo var. makuwa])

HSP 1 Score: 426.4 bits (1095), Expect = 1.6e-115
Identity = 207/239 (86.61%), Postives = 225/239 (94.14%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PPTLNPS+GAGH+EQLGGL+TYV+GSPDSK+AV+FITDV+G+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKIAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDP+VPDDANRPI+VW QDHG DK FDDAK VVEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHGPDKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVVEL+K ELIQAAVLLHPS VTVDDIKGVKA IS+LGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAHISILGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEEILS+  EVD FVKIFPKVSHGWTVRY+VEDEEAVK ADEAH DLLAWFTKY+K
Sbjct: 181 LKQFEEILSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Lag0021317 vs. NCBI nr
Match: XP_004134157.1 (endo-1,3;1,4-beta-D-glucanase [Cucumis sativus] >KGN57031.1 hypothetical protein Csa_011638 [Cucumis sativus])

HSP 1 Score: 423.7 bits (1088), Expect = 1.1e-114
Identity = 205/239 (85.77%), Postives = 224/239 (93.72%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PPTLNPS+GAGH+EQLGGL+TYV+GSPDSKLAV+FITDV+G+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKLAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDP+VPDDANRPI+VW QDH  +K FDDAK VVEALK+KGI+A
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHETEKGFDDAKPVVEALKNKGITA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVVEL+K ELIQAAVLLHPS VTVDDIKGVKAPIS+LGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAPISILGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LK+FEEILS+  EVD FVKIFPKVSHGWTVRY VEDEEAVK ADEAH DLLAWFTKY+K
Sbjct: 181 LKEFEEILSAKPEVDGFVKIFPKVSHGWTVRYKVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Lag0021317 vs. NCBI nr
Match: XP_038879789.1 (endo-1,3;1,4-beta-D-glucanase-like [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 6.8e-114
Identity = 205/239 (85.77%), Postives = 226/239 (94.56%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           M+G QCCS+PPTLNPS+GAGH+E+LGGL+TYVTGSPD KLAV+FITDV+G+EAPLLRKLA
Sbjct: 1   MAGPQCCSNPPTLNPSSGAGHLEELGGLTTYVTGSPD-KLAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDP+VPDDANRPI+VW QDHG +K FDDAK VVEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVPDDANRPIRVWLQDHGPEKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVV+L+K ELIQAAVLLHPS VTVDDIKGVKA ISVLGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVDLTKVELIQAAVLLHPSFVTVDDIKGVKAHISVLGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LK+FEE+LS+  EVD FVKIFPKVSHGWTVRY++EDEEAVK ADEAHGDLLAWFTKYLK
Sbjct: 181 LKEFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNMEDEEAVKCADEAHGDLLAWFTKYLK 238

BLAST of Lag0021317 vs. NCBI nr
Match: XP_022137980.1 (endo-1,3;1,4-beta-D-glucanase-like [Momordica charantia])

HSP 1 Score: 420.6 bits (1080), Expect = 8.9e-114
Identity = 206/239 (86.19%), Postives = 219/239 (91.63%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PP LNPS GAGHVEQL GLSTYV+GSPDSKLAV+F+TDVFGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPALNPSGGAGHVEQLAGLSTYVSGSPDSKLAVIFVTDVFGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPY+PDDANRPI+ W QDHG DK F+DAK VVEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPYIPDDANRPIRTWLQDHGPDKGFEDAKPVVEALKSKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVG CWGAKVVV L+K ELIQAAVLLHPS VTVDDIK VKA ISVLGAEID ISPPEL
Sbjct: 121 IGAVGFCWGAKVVVALAKEELIQAAVLLHPSFVTVDDIKEVKAHISVLGAEIDQISPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEE+LS+  EVD FVKIFPKV HGW+VRY+VEDEEAVKHA+EAHGDLLAWFTKYLK
Sbjct: 181 LKQFEEVLSAKPEVDGFVKIFPKVHHGWSVRYNVEDEEAVKHAEEAHGDLLAWFTKYLK 239

BLAST of Lag0021317 vs. NCBI nr
Match: XP_023539324.1 (endo-1,3;1,4-beta-D-glucanase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.5 bits (1072), Expect = 7.5e-113
Identity = 206/239 (86.19%), Postives = 222/239 (92.89%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PP+LNPS+GAGHVEQLGGLSTYV+G+PDSK AV+FITDV+GYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPSSGAGHVEQLGGLSTYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPYV +D NRPIK W Q+HG +KSFDD KAVVEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPYVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVV L+K ELIQAAVLLHPSLVTVDDIK VKA +SVLGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVSVLGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEE L++  EVD FVKIFPKVSHGWTVRY+VEDEEAVK ADEAHGDLLAWFTKYLK
Sbjct: 181 LKQFEEALAAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTKYLK 239

BLAST of Lag0021317 vs. ExPASy Swiss-Prot
Match: Q9ZT66 (Endo-1,3;1,4-beta-D-glucanase OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 8.6e-35
Identity = 87/197 (44.16%), Postives = 117/197 (59.39%), Query Frame = 0

Query: 4   SQCCSSPPTLN----PSTGAGHV--EQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLR 63
           SQC  +PP  +        AG V  +  GGL  YV+G+  S  AV+  +DVFGYEAPLLR
Sbjct: 27  SQCLDNPPDRSIHGRQLAEAGEVVHDLPGGLRAYVSGAASSSRAVVLASDVFGYEAPLLR 86

Query: 64  KLADKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKG 123
           ++ DKVA AG+FVV PDF KGD Y+ D  N     W + H   K+ +DAK +  ALK +G
Sbjct: 87  QIVDKVAKAGYFVVVPDFLKGD-YLDDKKN--FTEWLEAHSPVKAAEDAKPLFAALKKEG 146

Query: 124 ISAIGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISP 183
             ++   G CWG K+ VE+ K   ++A  L HP  VT DD+K VK PI +LGA+ D  +P
Sbjct: 147 -KSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTP 206

Query: 184 PELLKQFEEILSSNSEV 195
           P+ + +F  +L    EV
Sbjct: 207 PKEVYRFVHVLRERHEV 219

BLAST of Lag0021317 vs. ExPASy Swiss-Prot
Match: A0A2V5GSC6 (Hydrolase pyvD OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=pyvD PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.6e-25
Identity = 72/241 (29.88%), Postives = 121/241 (50.21%), Query Frame = 0

Query: 4   SQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKL--AVMFITDVFGYEAPLLRKLAD 63
           SQCC    +L+     G + + G +  YV+  PD     A++F++D+FG      + +AD
Sbjct: 7   SQCCIG-GSLHEGETQGELTKFGDIPVYVSYPPDKSTHNAILFLSDIFGLALVNSKLIAD 66

Query: 64  KVAAAGFFVVAPDFFKGDPYVPDDANRPIKV--WFQDHGADKSFDDAKAVVEALKSK-GI 123
             AA G+ VV PD F+GDP VP D +   ++  W Q H   ++     A +  ++ + G 
Sbjct: 67  LFAANGYLVVMPDLFQGDP-VPVDHSPSFQIMDWLQGHLPPQTDPIVTATLREMRERLGC 126

Query: 124 SAIGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPP 183
             IG VG C+G K VV       +    + HP+ +  D++K ++ P+S+  +  D++ PP
Sbjct: 127 QRIGGVGYCYGGKYVVRYLHPGQLDVGFVAHPTFIEPDELKAIEGPLSISASAQDNLFPP 186

Query: 184 ELLKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYL 240
           E   + E IL +  +V   + IF  V HG+ VR  +         +++    + WF +YL
Sbjct: 187 EKRHESETIL-AQLDVPYQINIFSDVEHGFAVRCDLNKTRHRFAKEQSFSQGVTWFNQYL 244

BLAST of Lag0021317 vs. ExPASy Swiss-Prot
Match: Q6P7K0 (Carboxymethylenebutenolidase homolog OS=Xenopus tropicalis OX=8364 GN=cmbl PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.1e-24
Identity = 71/224 (31.70%), Postives = 115/224 (51.34%), Query Frame = 0

Query: 20  GHVEQLGGLSTYVTGSPDS-KLAVMFITDVFGYEAPLLRKLADKVAAAGFFVVAPDFFKG 79
           G   Q+  +  YV+    S   AV+ + D+FG++ P  R +AD + A G+  + PDFF G
Sbjct: 22  GQEVQIEHIKAYVSKPHSSTDKAVIVVQDIFGWQLPNTRFMADLLTAHGYITICPDFFVG 81

Query: 80  -DPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSK-GISAIGAVGICWGAKVVVEL 139
            +P+ P +       W Q   A K   +   V++ LK +  +  IG +G CWG  V   L
Sbjct: 82  QEPWKPSNDRSTFTEWLQTRQATKVEKEINVVLKYLKEQCHVKKIGVIGFCWGGVVTHHL 141

Query: 140 S-KFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPELLKQFEEILSSNSEVD 199
             K+  ++A V  +  +  V+D   +  P   + AE+DH+ P E +   EE L  +S+VD
Sbjct: 142 MLKYPELKAGVSFYGIIRDVEDRYNLLNPTLFIFAEMDHVIPLEQVSLLEEKLKVHSKVD 201

Query: 200 CFVKIFPKVSHGWTVRYSVE-DEEAVKHADEAHGDLLAWFTKYL 239
             VK+FPK +HG+  R + + + E     +EA  ++L W  KY+
Sbjct: 202 FQVKVFPKQTHGFVHRKNEDINPEDKPFIEEARKNMLEWLHKYI 245

BLAST of Lag0021317 vs. ExPASy Swiss-Prot
Match: Q5XH09 (Carboxymethylenebutenolidase homolog OS=Xenopus laevis OX=8355 GN=cmbl PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.4e-23
Identity = 70/224 (31.25%), Postives = 113/224 (50.45%), Query Frame = 0

Query: 20  GHVEQLGGLSTYVTGSPDS-KLAVMFITDVFGYEAPLLRKLADKVAAAGFFVVAPDFFKG 79
           G   Q+  +  YV+    S   AV+ + D+FG++ P  R +AD + A G+  + PDFF G
Sbjct: 22  GQEIQIEHIKAYVSKPHSSTDKAVIVVQDIFGWQLPNTRFMADLLTAHGYITICPDFFVG 81

Query: 80  -DPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSK-GISAIGAVGICWGAKVVVEL 139
            + + P +       W Q   A K   +   V++ LK +  +  IG +G CWG  V   L
Sbjct: 82  QESWKPSNDWSTFTEWLQTRQATKVEKEMNVVLKYLKEQCHVKKIGVIGFCWGGVVTHHL 141

Query: 140 S-KFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPELLKQFEEILSSNSEVD 199
             K+  ++A V  +  +  V+D   +  P   + AEIDH+ P E +   E+ L  +S+VD
Sbjct: 142 MLKYPELKAGVSFYGIIRDVEDRYNLLNPTLFIFAEIDHVIPLEQVSLLEQKLKVHSKVD 201

Query: 200 CFVKIFPKVSHGWTVRYSVE-DEEAVKHADEAHGDLLAWFTKYL 239
             +K+FPK +HG+  R   + + E     +EA  D+L W  KY+
Sbjct: 202 FQIKVFPKQTHGFVHRKKEDINPEDKPFIEEARKDMLEWLQKYI 245

BLAST of Lag0021317 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.1e-21
Identity = 70/226 (30.97%), Postives = 114/226 (50.44%), Query Frame = 0

Query: 18  GAGHVEQLGGLSTYVTGSP-DSKLAVMFITDVFGYEAPLLRKLADKVAAAGFFVVAPDFF 77
           G GH  Q+  +  YVT SP D+  AV+ + D+FG++ P  R +AD +A  G+  + PDFF
Sbjct: 20  GMGHEVQVEHIKAYVTRSPVDAGKAVIVVQDIFGWQLPNTRYMADMIARNGYTTIVPDFF 79

Query: 78  KG-DPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSK-GISAIGAVGICWGAKVVV 137
            G +P+ P         W +   A K   +  AV+  L+ +     IG VG CWG  VV 
Sbjct: 80  VGQEPWDPAGDWSTFPAWLKSRNARKVNREVDAVLRYLRQQCHAQKIGIVGFCWGGVVVH 139

Query: 138 E-LSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPELLKQFEEILSSNSE 197
           + ++ +  I+A V ++  +   +D+  +K P   + AE D + P E +    + L  +  
Sbjct: 140 QVMTAYPDIRAGVSVYGIIRDSEDVYNLKNPTLFIFAENDTVIPLEQVSTLTQKLKEHCI 199

Query: 198 VDCFVKIFPKVSHGWTVRYSVEDEEAVK-HADEAHGDLLAWFTKYL 239
           V+  VK F   +HG+  R   +   A K + +EA  +L+ W  KY+
Sbjct: 200 VNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEARRNLIEWLNKYV 245

BLAST of Lag0021317 vs. ExPASy TrEMBL
Match: A0A5D3CVZ9 (Endo-1,3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00010 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 7.9e-116
Identity = 207/239 (86.61%), Postives = 225/239 (94.14%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PPTLNPS+GAGH+EQLGGL+TYV+GSPDSK+AV+FITDV+G+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKIAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDP+VPDDANRPI+VW QDHG DK FDDAK VVEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHGPDKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVVEL+K ELIQAAVLLHPS VTVDDIKGVKA IS+LGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAHISILGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEEILS+  EVD FVKIFPKVSHGWTVRY+VEDEEAVK ADEAH DLLAWFTKY+K
Sbjct: 181 LKQFEEILSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Lag0021317 vs. ExPASy TrEMBL
Match: A0A1S3AWR8 (endo-1,31,4-beta-D-glucanase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483753 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 7.9e-116
Identity = 207/239 (86.61%), Postives = 225/239 (94.14%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PPTLNPS+GAGH+EQLGGL+TYV+GSPDSK+AV+FITDV+G+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKIAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDP+VPDDANRPI+VW QDHG DK FDDAK VVEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHGPDKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVVEL+K ELIQAAVLLHPS VTVDDIKGVKA IS+LGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAHISILGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEEILS+  EVD FVKIFPKVSHGWTVRY+VEDEEAVK ADEAH DLLAWFTKY+K
Sbjct: 181 LKQFEEILSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Lag0021317 vs. ExPASy TrEMBL
Match: A0A0A0LAG0 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G150110 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.1e-115
Identity = 205/239 (85.77%), Postives = 224/239 (93.72%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PPTLNPS+GAGH+EQLGGL+TYV+GSPDSKLAV+FITDV+G+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKLAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDP+VPDDANRPI+VW QDH  +K FDDAK VVEALK+KGI+A
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHETEKGFDDAKPVVEALKNKGITA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVVEL+K ELIQAAVLLHPS VTVDDIKGVKAPIS+LGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAPISILGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LK+FEEILS+  EVD FVKIFPKVSHGWTVRY VEDEEAVK ADEAH DLLAWFTKY+K
Sbjct: 181 LKEFEEILSAKPEVDGFVKIFPKVSHGWTVRYKVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Lag0021317 vs. ExPASy TrEMBL
Match: A0A6J1C860 (endo-1,31,4-beta-D-glucanase-like OS=Momordica charantia OX=3673 GN=LOC111009255 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.3e-114
Identity = 206/239 (86.19%), Postives = 219/239 (91.63%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PP LNPS GAGHVEQL GLSTYV+GSPDSKLAV+F+TDVFGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPALNPSGGAGHVEQLAGLSTYVSGSPDSKLAVIFVTDVFGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPY+PDDANRPI+ W QDHG DK F+DAK VVEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPYIPDDANRPIRTWLQDHGPDKGFEDAKPVVEALKSKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVG CWGAKVVV L+K ELIQAAVLLHPS VTVDDIK VKA ISVLGAEID ISPPEL
Sbjct: 121 IGAVGFCWGAKVVVALAKEELIQAAVLLHPSFVTVDDIKEVKAHISVLGAEIDQISPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEE+LS+  EVD FVKIFPKV HGW+VRY+VEDEEAVKHA+EAHGDLLAWFTKYLK
Sbjct: 181 LKQFEEVLSAKPEVDGFVKIFPKVHHGWSVRYNVEDEEAVKHAEEAHGDLLAWFTKYLK 239

BLAST of Lag0021317 vs. ExPASy TrEMBL
Match: A0A6J1F9P2 (endo-1,31,4-beta-D-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111443361 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.0e-112
Identity = 203/239 (84.94%), Postives = 221/239 (92.47%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCCS+PP+LNP++GAGHVEQLGGLS YV+G+PDSK AV+FITDV+GYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPTSGAGHVEQLGGLSAYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDP+V +D NRPIK W Q+HG +KSFDD KAVVEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGAVGICWGAKVVV L+K ELIQAAVLLHPSLVTVDDIK VKA +SVLGAEIDH+SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVSVLGAEIDHMSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEE L++  EVD FVKIFPKVSHGWTVRY+VEDEEAVK ADEAHGDLLAWFTKYLK
Sbjct: 181 LKQFEEALAAKPEVDAFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTKYLK 239

BLAST of Lag0021317 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 3.1e-96
Identity = 164/239 (68.62%), Postives = 200/239 (83.68%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCC +PPTLNP +G+GHVE+LGGL  YV+GS +SKL V+ I+D+FG+EAP LR LA
Sbjct: 1   MSGPQCCENPPTLNPVSGSGHVEKLGGLDAYVSGSAESKLCVLLISDIFGFEAPNLRALA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAA+GF+VV PD+F GDPY P + +RPI VW +DHG DK F++ K V+E +K+KGI+A
Sbjct: 61  DKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIKNKGITA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGA G+CWGAKVVVELSK ELIQAAVLLHPS V VDDIKG KAPI++LGAEID +SPP L
Sbjct: 121 IGAAGMCWGAKVVVELSKEELIQAAVLLHPSFVNVDDIKGGKAPIAILGAEIDQMSPPAL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEEILSS  EV+ +VKI PKVSHGWTVRY++++ EAVK A+EAH ++L WF  Y+K
Sbjct: 181 LKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWFVTYIK 239

BLAST of Lag0021317 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 3.2e-93
Identity = 161/239 (67.36%), Postives = 197/239 (82.43%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QCC +PPTLNP +G+GHVE+LGGL  YV+GS +SKL V+ I+D+FG+EAP LR LA
Sbjct: 1   MSGPQCCENPPTLNPVSGSGHVEKLGGLDAYVSGSAESKLCVLLISDIFGFEAPNLRALA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVAA+GF+VV PD+F GDPY P + +RPI VW +DHG DK F++ K V+E +K+KGI+A
Sbjct: 61  DKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIKNKGITA 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGA G+CWG   VVELSK ELIQAAVLLHPS V VDDIKG KAPI++LGAEID +SPP L
Sbjct: 121 IGAAGMCWG---VVELSKEELIQAAVLLHPSFVNVDDIKGGKAPIAILGAEIDQMSPPAL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           LKQFEEILSS  EV+ +VKI PKVSHGWTVRY++++ EAVK A+EAH ++L WF  Y+K
Sbjct: 181 LKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWFVTYIK 236

BLAST of Lag0021317 vs. TAIR 10
Match: AT3G23570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 2.4e-85
Identity = 147/239 (61.51%), Postives = 184/239 (76.99%), Query Frame = 0

Query: 1   MSGSQCCSSPPTLNPSTGAGHVEQLGGLSTYVTGSPDSKLAVMFITDVFGYEAPLLRKLA 60
           MSG QC  +PP L+P++G+GHVE+LG L TYV GS  SKLAV+ +  VFGYE P LRKLA
Sbjct: 1   MSGHQCTENPPDLDPTSGSGHVEKLGNLDTYVCGSTHSKLAVLLVPHVFGYETPNLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADKSFDDAKAVVEALKSKGISA 120
           DKVA AGF+ V PDFF GDPY P++ +RP  +W +DH  +K F+++K +VEALK+KGI++
Sbjct: 61  DKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELEKGFEESKPIVEALKNKGITS 120

Query: 121 IGAVGICWGAKVVVELSKFELIQAAVLLHPSLVTVDDIKGVKAPISVLGAEIDHISPPEL 180
           IGA G CWGAKV VEL+K +L+ A VLLHP+ VTVDDIK V  PI+VLGAEID +SPPEL
Sbjct: 121 IGAAGFCWGAKVAVELAKEKLVDATVLLHPARVTVDDIKEVNLPIAVLGAEIDQVSPPEL 180

Query: 181 LKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYSVEDEEAVKHADEAHGDLLAWFTKYLK 240
           ++QFE+IL+S  +V  FVKIFP+  HGWTVRY+  D   V+ A EAH D+LAW   YLK
Sbjct: 181 VRQFEDILASKPQVKSFVKIFPRCKHGWTVRYNENDPSEVEAAMEAHKDMLAWLIDYLK 239

BLAST of Lag0021317 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-11
Identity = 49/205 (23.90%), Postives = 94/205 (45.85%), Query Frame = 0

Query: 42  VMFITDVFGYEAPLLRKLADKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADK 101
           ++ ++DVFG++    R  A +VA  G+ V+ PD F+GDP+  +      + W + H  ++
Sbjct: 111 LLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNR 170

Query: 102 SFDD----AKAVVEALKSKGIS-AIGAVGICWGAKVVVEL---SKFELIQAAVLLHPSLV 161
              D     K +VE   + GIS  +G +G C+G   VV++    +       V  + + +
Sbjct: 171 IRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRI 230

Query: 162 TVDDIKGVKAPISVLGAEIDHISPPELLKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYS 221
                  VK P+  +  + D +   + L + EE +   S+    V ++    HG+  R  
Sbjct: 231 DSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSK----VVVYEGRGHGFVHRPE 290

Query: 222 VEDEEAVKHADEAHGDLLAWFTKYL 239
             +++  + A+EA   +  W   +L
Sbjct: 291 TPEDD--RDAEEAFALMRNWLHHHL 309

BLAST of Lag0021317 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-11
Identity = 49/205 (23.90%), Postives = 94/205 (45.85%), Query Frame = 0

Query: 42  VMFITDVFGYEAPLLRKLADKVAAAGFFVVAPDFFKGDPYVPDDANRPIKVWFQDHGADK 101
           ++ ++DVFG++    R  A +VA  G+ V+ PD F+GDP+  +      + W + H  ++
Sbjct: 116 LLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNR 175

Query: 102 SFDD----AKAVVEALKSKGIS-AIGAVGICWGAKVVVEL---SKFELIQAAVLLHPSLV 161
              D     K +VE   + GIS  +G +G C+G   VV++    +       V  + + +
Sbjct: 176 IRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRI 235

Query: 162 TVDDIKGVKAPISVLGAEIDHISPPELLKQFEEILSSNSEVDCFVKIFPKVSHGWTVRYS 221
                  VK P+  +  + D +   + L + EE +   S+    V ++    HG+  R  
Sbjct: 236 DSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSK----VVVYEGRGHGFVHRPE 295

Query: 222 VEDEEAVKHADEAHGDLLAWFTKYL 239
             +++  + A+EA   +  W   +L
Sbjct: 296 TPEDD--RDAEEAFALMRNWLHHHL 314