Lag0021268 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021268
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionHeat shock 70 kDa protein 8
Locationchr7: 6004913 .. 6006628 (-)
RNA-Seq ExpressionLag0021268
SyntenyLag0021268
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAACCAATATACACTGTAGCTTCCGATAGTGAAACCACTGGGGAGGAAAAAACAACATCAACCTTTCCGGAGATTGCGGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAATCTGGAATGGATCTCAGATAGAGCTTCTTAAGAACACTAGAAATCAGAAAATGATGAGATCATATGTCACCTTCAAGGATGATACACCTTCTGGAGGAGTTAGTAATGAGCTTAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGCTTGATTGGAAGAGTTGACACTGATCCAGTTGTTCATGCAAGCAAGAGCCTTCCTTTTCTTGTGCAAACTTTGGACGTTGGTGTTCGTCCATTTGTTGCAGCTCTGGTGAATAATGTATGGAGATCGACCACTCCTGAAGAAGTTCTGGCGATCTTTCTCGTTGAACTGAAAATGATGGCTGAATATCAACTAAAGCGGCCAATTAGAAATGTTGTTTTGACAATTCCAGTTTCATTCAGCCGGTTCCAGCTTACTCGGGTTGAACGAGCTTGTGCAATGGCTGGCCTTCAAGTCAGGCTGATGCCTGAACCAACAGCCATAGCTTTATTATATGCCCAACAACAGCAGCAGGCTGTACATGAGAACATGGGCAGTGGAAGCGAAAAAATTGCCCTTATTTTCAACATGGGAGCTGGGTATTGTGATGTTGCTGTATCAGCTACAGGCGGAGGAGTGTCACAGATCAGAGCCCTGGCTGGAAGTCCTATTGGAGGTGAAGATCTACTTCAAAATACCATGAAGTATCTTTTGCCGAATTCTGAATCTATATTCTCACACCGTGGAATTGATGAGATCAGAAGGATGGGATTGCTCCGAGTTGCAACCCAAGATGCTATCCACAAGCTTTCATTCCAGTCAAATGTTCAAATCGATGTCAACTTGGGAAATGGATCAAAAATATGCAAGGTCCTAAGCCGGGACGAGTTTGAGATGGTGAACAGCAAGGTGTTTGACAAATGTGAGAACCTCGTAAAGCAATGCTTACACGATGCAAGAGTAGAGATTCAAGAGGTGAGCGACGTGGTAGTCGTTGGTGGATGTTCATACATACCAAAAATCAGAAAACTTATGATGGAAATAACTAAAAAGAAGGCACTTTATGAAGGAATGGATCCTTTAGAAGCTGCTGTTTCTGGTGCAGCCTTAGAAGGTGCGGTGGCATCAGGCATCGGCGATCCATTTGGAAGTTTAGATCTTCTTAGCATCCAAGCGACGCCTCTAGCCATTGGAATTCGAGCTGATGGAAACAGCTTCATTCCAATTATTCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTGGTTTTCACAACCGTTCAAGATAATCAAAGCGAGGCACTGATCCTTGTCTATGAAGGCGAAGAGACAAAAGCAGAAGAAAACCATCTGCTTGGCTATTTCAAGATCATTGGAATTCCACCAGCACCCAAAGGAGTCCCTGAAATTACTATTTGCATGGACATTGATTCTTCAAATATGCTAAGAGTTCTAGCTGGGGCAACACTCCCAGGTTCTCAACACCCTGCAACTCCTTTCATGGAAGTCAAGATGCCGACCGTAGACGACGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATACGGTGCCGCCATGGACTTGGTTACATTGCGGAAGAAAATCTAG

mRNA sequence

ATGGCTGAACCAATATACACTGTAGCTTCCGATAGTGAAACCACTGGGGAGGAAAAAACAACATCAACCTTTCCGGAGATTGCGGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAATCTGGAATGGATCTCAGATAGAGCTTCTTAAGAACACTAGAAATCAGAAAATGATGAGATCATATGTCACCTTCAAGGATGATACACCTTCTGGAGGAGTTAGTAATGAGCTTAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGCTTGATTGGAAGAGTTGACACTGATCCAGTTGTTCATGCAAGCAAGAGCCTTCCTTTTCTTGTGCAAACTTTGGACGTTGGTGTTCGTCCATTTGTTGCAGCTCTGGTGAATAATGTATGGAGATCGACCACTCCTGAAGAAGTTCTGGCGATCTTTCTCGTTGAACTGAAAATGATGGCTGAATATCAACTAAAGCGGCCAATTAGAAATGTTGTTTTGACAATTCCAGTTTCATTCAGCCGGTTCCAGCTTACTCGGGTTGAACGAGCTTGTGCAATGGCTGGCCTTCAAGTCAGGCTGATGCCTGAACCAACAGCCATAGCTTTATTATATGCCCAACAACAGCAGCAGGCTGTACATGAGAACATGGGCAGTGGAAGCGAAAAAATTGCCCTTATTTTCAACATGGGAGCTGGGTATTGTGATGTTGCTGTATCAGCTACAGGCGGAGGAGTGTCACAGATCAGAGCCCTGGCTGGAAGTCCTATTGGAGGTGAAGATCTACTTCAAAATACCATGAAGTATCTTTTGCCGAATTCTGAATCTATATTCTCACACCGTGGAATTGATGAGATCAGAAGGATGGGATTGCTCCGAGTTGCAACCCAAGATGCTATCCACAAGCTTTCATTCCAGTCAAATGTTCAAATCGATGTCAACTTGGGAAATGGATCAAAAATATGCAAGGTCCTAAGCCGGGACGAGTTTGAGATGGTGAACAGCAAGGTGTTTGACAAATGTGAGAACCTCGTAAAGCAATGCTTACACGATGCAAGAGTAGAGATTCAAGAGGTGAGCGACGTGGTAGTCGTTGGTGGATGTTCATACATACCAAAAATCAGAAAACTTATGATGGAAATAACTAAAAAGAAGGCACTTTATGAAGGAATGGATCCTTTAGAAGCTGCTGTTTCTGGTGCAGCCTTAGAAGGTGCGGTGGCATCAGGCATCGGCGATCCATTTGGAAGTTTAGATCTTCTTAGCATCCAAGCGACGCCTCTAGCCATTGGAATTCGAGCTGATGGAAACAGCTTCATTCCAATTATTCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTGGTTTTCACAACCGTTCAAGATAATCAAAGCGAGGCACTGATCCTTGTCTATGAAGGCGAAGAGACAAAAGCAGAAGAAAACCATCTGCTTGGCTATTTCAAGATCATTGGAATTCCACCAGCACCCAAAGGAGTCCCTGAAATTACTATTTGCATGGACATTGATTCTTCAAATATGCTAAGAGTTCTAGCTGGGGCAACACTCCCAGGTTCTCAACACCCTGCAACTCCTTTCATGGAAGTCAAGATGCCGACCGTAGACGACGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATACGGTGCCGCCATGGACTTGGTTACATTGCGGAAGAAAATCTAG

Coding sequence (CDS)

ATGGCTGAACCAATATACACTGTAGCTTCCGATAGTGAAACCACTGGGGAGGAAAAAACAACATCAACCTTTCCGGAGATTGCGGTTGGAATTGATATTGGTACTTCACAATGTAGTATTGCAATCTGGAATGGATCTCAGATAGAGCTTCTTAAGAACACTAGAAATCAGAAAATGATGAGATCATATGTCACCTTCAAGGATGATACACCTTCTGGAGGAGTTAGTAATGAGCTTAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGCTTGATTGGAAGAGTTGACACTGATCCAGTTGTTCATGCAAGCAAGAGCCTTCCTTTTCTTGTGCAAACTTTGGACGTTGGTGTTCGTCCATTTGTTGCAGCTCTGGTGAATAATGTATGGAGATCGACCACTCCTGAAGAAGTTCTGGCGATCTTTCTCGTTGAACTGAAAATGATGGCTGAATATCAACTAAAGCGGCCAATTAGAAATGTTGTTTTGACAATTCCAGTTTCATTCAGCCGGTTCCAGCTTACTCGGGTTGAACGAGCTTGTGCAATGGCTGGCCTTCAAGTCAGGCTGATGCCTGAACCAACAGCCATAGCTTTATTATATGCCCAACAACAGCAGCAGGCTGTACATGAGAACATGGGCAGTGGAAGCGAAAAAATTGCCCTTATTTTCAACATGGGAGCTGGGTATTGTGATGTTGCTGTATCAGCTACAGGCGGAGGAGTGTCACAGATCAGAGCCCTGGCTGGAAGTCCTATTGGAGGTGAAGATCTACTTCAAAATACCATGAAGTATCTTTTGCCGAATTCTGAATCTATATTCTCACACCGTGGAATTGATGAGATCAGAAGGATGGGATTGCTCCGAGTTGCAACCCAAGATGCTATCCACAAGCTTTCATTCCAGTCAAATGTTCAAATCGATGTCAACTTGGGAAATGGATCAAAAATATGCAAGGTCCTAAGCCGGGACGAGTTTGAGATGGTGAACAGCAAGGTGTTTGACAAATGTGAGAACCTCGTAAAGCAATGCTTACACGATGCAAGAGTAGAGATTCAAGAGGTGAGCGACGTGGTAGTCGTTGGTGGATGTTCATACATACCAAAAATCAGAAAACTTATGATGGAAATAACTAAAAAGAAGGCACTTTATGAAGGAATGGATCCTTTAGAAGCTGCTGTTTCTGGTGCAGCCTTAGAAGGTGCGGTGGCATCAGGCATCGGCGATCCATTTGGAAGTTTAGATCTTCTTAGCATCCAAGCGACGCCTCTAGCCATTGGAATTCGAGCTGATGGAAACAGCTTCATTCCAATTATTCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTGGTTTTCACAACCGTTCAAGATAATCAAAGCGAGGCACTGATCCTTGTCTATGAAGGCGAAGAGACAAAAGCAGAAGAAAACCATCTGCTTGGCTATTTCAAGATCATTGGAATTCCACCAGCACCCAAAGGAGTCCCTGAAATTACTATTTGCATGGACATTGATTCTTCAAATATGCTAAGAGTTCTAGCTGGGGCAACACTCCCAGGTTCTCAACACCCTGCAACTCCTTTCATGGAAGTCAAGATGCCGACCGTAGACGACGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATACGGTGCCGCCATGGACTTGGTTACATTGCGGAAGAAAATCTAG

Protein sequence

MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKKI
Homology
BLAST of Lag0021268 vs. NCBI nr
Match: XP_004134140.1 (heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa protein 8 [Cucumis sativus] >KGN56991.1 hypothetical protein Csa_011014 [Cucumis sativus])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.2e-309
Identity = 533/570 (93.51%), Postives = 565/570 (99.12%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQQQAVH++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR++FEMVNSKVF+KCENLVKQCLHDAR+ I++
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIR L+MEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYGA M+LVTLRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Lag0021268 vs. NCBI nr
Match: XP_038897373.1 (heat shock 70 kDa protein 8 [Benincasa hispida])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.6e-309
Identity = 535/570 (93.86%), Postives = 561/570 (98.42%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE+KTTSTFP+IAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDKTTSTFPDIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHE+DMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEFDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKR I+NVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRSIKNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQQQAVHE+MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHESMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+VQIDVNLGNGSK+CKVLSR+EFEMVNSKVF+KCENLVKQCLHDARV I++
Sbjct: 301 AIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVRIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIR L+MEI K KALYEGMDPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRNLLMEICKNKALYEGMDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTV+DNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVRDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGY KIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATP+MEVKM
Sbjct: 481 AEENHLLGYVKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYG  M+LV+LRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGPTMELVSLRKK 570

BLAST of Lag0021268 vs. NCBI nr
Match: KAA0049367.1 (heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70 kDa protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.7e-308
Identity = 532/570 (93.33%), Postives = 562/570 (98.60%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVF+KCENLVKQCLHDARV I++
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIRKL+MEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYGA M+LVTLRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Lag0021268 vs. NCBI nr
Match: XP_008438682.1 (PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.6e-307
Identity = 531/570 (93.16%), Postives = 561/570 (98.42%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVF+KCENLVKQCLHDARV I++
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIRKL+MEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYGA M+LVTLRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Lag0021268 vs. NCBI nr
Match: XP_022138266.1 (heat shock 70 kDa protein 8 [Momordica charantia])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.7e-304
Identity = 528/570 (92.63%), Postives = 556/570 (97.54%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVS+EL HEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQ QAVHENMGSGSEKIAL+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           +SAT GGVSQIRAL GSPIGGEDLLQNTMKYLLPNSESIFS+ GIDEIRRMGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AI KLSFQ++ QIDVNLGNGSKICKVLSR+EFEMVNSKVF+KCENLVKQCLHDARVEI++
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCS IPKIRKL+MEI+KKKALYE +DPLEAAVSGAA+EGAVASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQ TPLAIGIRADGN+FIPIIPKNTTMPARKELVFTTVQDNQSEALI+VYEGEE +
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSN LRVLAGATLPG+QHPATPFMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PT+DDGH WCAEALHGK+G +MDLVTLRKK
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKK 570

BLAST of Lag0021268 vs. ExPASy Swiss-Prot
Match: Q9SKY8 (Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 2.1e-244
Identity = 413/574 (71.95%), Postives = 495/574 (86.24%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTS--TFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQK 60
           MAE  YTVASDSE TGEEK++S  + PEIA+GIDIGTSQCSIA+WNGSQ+ +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  MMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQ 120
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRF 180
           TLD+GVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGLQV-RLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYC 240
           QLTR ERACAMAGL V RLMPEPTAIALLYAQQQQ   H+NMGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 300
           DVAV+AT GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVE 360
            QDAIH+L+ Q NVQI+V+LGNG+KI KVL R EFE VN KVF++CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IQEVSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPF 420
             ++ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGE 480
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFME 540
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PGS  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKKI 572
           V+MPTVDDGHGWCA+AL+ KYGA +DL+TL++K+
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRKM 563

BLAST of Lag0021268 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.2e-76
Identity = 175/511 (34.25%), Postives = 286/511 (55.97%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHASKS-LPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 148 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 207
           + L++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 267
            Y       + +   S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AY------GLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 327
               + + +   +           R +  LR A + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 328 ICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKIRKLMME 387
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++L+ +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Lag0021268 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 5.3e-75
Identity = 178/515 (34.56%), Postives = 284/515 (55.15%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +DP V +  K  PF V     G +P +        +    EE+ +
Sbjct: 69  INTVFDAKRLIGRRFSDPSVQSDIKLWPFKV-IPGPGDKPMIVVTYKGEEKQFAAEEISS 128

Query: 148 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 207
           + L ++K +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 267
            Y       + +   S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AY------GLDKKASSAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEI----RRMGLLRVATQDAIHKLS--FQSNVQIDVNLG 327
               + + +      F  +   +I    R +  LR A + A   LS   Q+ ++ID +L 
Sbjct: 249 DNRMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLY 308

Query: 328 NGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKIRK 387
            G      ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++
Sbjct: 309 EGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQ 368

Query: 388 LMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADG 447
           L+ +    K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G
Sbjct: 369 LLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAG 428

Query: 448 NSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIPPA 507
                +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPA
Sbjct: 429 GGMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 488

Query: 508 PKGVPEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           P+GVP+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Lag0021268 vs. ExPASy Swiss-Prot
Match: Q9S9N1 (Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.5e-74
Identity = 177/517 (34.24%), Postives = 284/517 (54.93%), Query Frame = 0

Query: 22  STFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSH 81
           +T  E A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 82  EYDMLSGAAIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDVGVRPFVAALVNNVWRSTT 141
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 142 PEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPE 201
           PEE+ ++ LV++K +AE  L R ++N V+T+P  F+  Q    + A A++GL V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 202 PTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SP 261
           PTA A+ Y   ++           EK  LIF++G G  DV++     GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 262 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVN 321
           +GGED     + + +               R +  LR A + A   LS   Q+ ++ID +
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 322 LGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKI 381
           L  G      +SR  FE +N  +F KC + V++ L DA+++   V DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 382 RKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRA 441
           ++L+ +    K L + ++P EA   GAA++ A+ +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 442 DGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIP 501
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  +  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 502 PAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           PAP+GVP+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Lag0021268 vs. ExPASy Swiss-Prot
Match: P22953 (Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3)

HSP 1 Score: 280.0 bits (715), Expect = 5.9e-74
Identity = 173/511 (33.86%), Postives = 282/511 (55.19%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHASKSL-PFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +D  V +   L PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 148 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 207
           + L++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 267
            Y   ++        S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 327
               + + +   +           R +  LR + + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 328 ICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKIRKLMME 387
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++L+ +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + ++P EA   GAA++GA+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Lag0021268 vs. ExPASy TrEMBL
Match: A0A0A0LAC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 533/570 (93.51%), Postives = 565/570 (99.12%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQQQAVH++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR++FEMVNSKVF+KCENLVKQCLHDAR+ I++
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIR L+MEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYGA M+LVTLRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Lag0021268 vs. ExPASy TrEMBL
Match: A0A5D3D052 (Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00370 PE=3 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.7e-308
Identity = 532/570 (93.33%), Postives = 562/570 (98.60%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVF+KCENLVKQCLHDARV I++
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIRKL+MEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYGA M+LVTLRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Lag0021268 vs. ExPASy TrEMBL
Match: A0A1S3AXP0 (heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 8.0e-308
Identity = 531/570 (93.16%), Postives = 561/570 (98.42%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVF+KCENLVKQCLHDARV I++
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCSYIPKIRKL+MEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALI+VYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PTVDDGHGWCAEALHGKYGA M+LVTLRKK
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKK 570

BLAST of Lag0021268 vs. ExPASy TrEMBL
Match: A0A6J1C992 (heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.2e-305
Identity = 528/570 (92.63%), Postives = 556/570 (97.54%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGVS+EL HEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 240
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQ QAVHENMGSGSEKIAL+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 241 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           +SAT GGVSQIRAL GSPIGGEDLLQNTMKYLLPNSESIFS+ GIDEIRRMGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQE 360
           AI KLSFQ++ QIDVNLGNGSKICKVLSR+EFEMVNSKVF+KCENLVKQCLHDARVEI++
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 361 VSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 420
           +SDVVVVGGCS IPKIRKL+MEI+KKKALYE +DPLEAAVSGAA+EGAVASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETK 480
           DLLSIQ TPLAIGIRADGN+FIPIIPKNTTMPARKELVFTTVQDNQSEALI+VYEGEE +
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSN LRVLAGATLPG+QHPATPFMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           PT+DDGH WCAEALHGK+G +MDLVTLRKK
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKK 570

BLAST of Lag0021268 vs. ExPASy TrEMBL
Match: A0A6J1GXD4 (heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 3.8e-302
Identity = 527/571 (92.29%), Postives = 555/571 (97.20%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 60
           MAEPIYTVASDSETTGE+++ S FPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRSASIFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120
           RSYVTFKDDTPSGGV NELS EYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVINELSQEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           D+GVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAE+QLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNIWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGLQVRLMPEPTAIALLYA-QQQQQAVHENMGSGSEKIALIFNMGAGYCDV 240
           TRVERACAMAGLQVRLMPEPTA+ALLYA QQQQQAVHENMGSGSEKIALIFNMGAGYCDV
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAVHENMGSGSEKIALIFNMGAGYCDV 240

Query: 241 AVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQ 300
           AVSATGGGVSQI+ALAGSPIGGEDLLQNTMKYLLPN+ESIFSHRGIDEIRRMGLLRVATQ
Sbjct: 241 AVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNAESIFSHRGIDEIRRMGLLRVATQ 300

Query: 301 DAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQ 360
           DAIHKLSFQS+VQIDVNLGNGSK+CKVLSR+EFEMVNS+VF+KCENLV QCLHDARV I 
Sbjct: 301 DAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCENLVNQCLHDARVGID 360

Query: 361 EVSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGS 420
           ++SDVVVVGGCS IPKIR  +ME +KKKALYEGM+PLEAAV GAALEGAVASGI DPFGS
Sbjct: 361 DLSDVVVVGGCSNIPKIRSFLMEKSKKKALYEGMNPLEAAVCGAALEGAVASGIADPFGS 420

Query: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEET 480
           LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTV+DNQSEALI+VYEGEET
Sbjct: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEGEET 480

Query: 481 KAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFMEVK 540
           +AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPG+QHPATPFMEVK
Sbjct: 481 RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGARLPGAQHPATPFMEVK 540

Query: 541 MPTVDDGHGWCAEALHGKYGAAMDLVTLRKK 571
           MPTVDDG GWCAEALHGKYGAAMDLV+LRKK
Sbjct: 541 MPTVDDGQGWCAEALHGKYGAAMDLVSLRKK 571

BLAST of Lag0021268 vs. TAIR 10
Match: AT2G32120.1 (heat-shock protein 70T-2 )

HSP 1 Score: 846.3 bits (2185), Expect = 1.5e-245
Identity = 413/574 (71.95%), Postives = 495/574 (86.24%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTS--TFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQK 60
           MAE  YTVASDSE TGEEK++S  + PEIA+GIDIGTSQCSIA+WNGSQ+ +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  MMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQ 120
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRF 180
           TLD+GVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGLQV-RLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYC 240
           QLTR ERACAMAGL V RLMPEPTAIALLYAQQQQ   H+NMGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 300
           DVAV+AT GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVE 360
            QDAIH+L+ Q NVQI+V+LGNG+KI KVL R EFE VN KVF++CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IQEVSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPF 420
             ++ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGE 480
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFME 540
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PGS  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKKI 572
           V+MPTVDDGHGWCA+AL+ KYGA +DL+TL++K+
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRKM 563

BLAST of Lag0021268 vs. TAIR 10
Match: AT2G32120.2 (heat-shock protein 70T-2 )

HSP 1 Score: 846.3 bits (2185), Expect = 1.5e-245
Identity = 413/574 (71.95%), Postives = 495/574 (86.24%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEEKTTS--TFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQK 60
           MAE  YTVASDSE TGEEK++S  + PEIA+GIDIGTSQCSIA+WNGSQ+ +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  MMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQ 120
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRF 180
           TLD+GVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGLQV-RLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYC 240
           QLTR ERACAMAGL V RLMPEPTAIALLYAQQQQ   H+NMGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 300
           DVAV+AT GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVE 360
            QDAIH+L+ Q NVQI+V+LGNG+KI KVL R EFE VN KVF++CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IQEVSDVVVVGGCSYIPKIRKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPF 420
             ++ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGE 480
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPFME 540
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PGS  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKKI 572
           V+MPTVDDGHGWCA+AL+ KYGA +DL+TL++K+
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRKM 563

BLAST of Lag0021268 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 175/511 (34.25%), Postives = 286/511 (55.97%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHASKS-LPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 148 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 207
           + L++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 267
            Y       + +   S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AY------GLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 327
               + + +   +           R +  LR A + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 328 ICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKIRKLMME 387
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++L+ +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Lag0021268 vs. TAIR 10
Match: AT1G16030.1 (heat shock protein 70B )

HSP 1 Score: 282.0 bits (720), Expect = 1.1e-75
Identity = 177/517 (34.24%), Postives = 284/517 (54.93%), Query Frame = 0

Query: 22  STFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSH 81
           +T  E A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 82  EYDMLSGAAIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDVGVRPFVAALVNNVWRSTT 141
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 142 PEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPE 201
           PEE+ ++ LV++K +AE  L R ++N V+T+P  F+  Q    + A A++GL V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 202 PTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SP 261
           PTA A+ Y   ++           EK  LIF++G G  DV++     GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 262 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVN 321
           +GGED     + + +               R +  LR A + A   LS   Q+ ++ID +
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 322 LGNGSKICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKI 381
           L  G      +SR  FE +N  +F KC + V++ L DA+++   V DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 382 RKLMMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRA 441
           ++L+ +    K L + ++P EA   GAA++ A+ +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 442 DGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIP 501
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  +  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 502 PAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           PAP+GVP+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Lag0021268 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 280.0 bits (715), Expect = 4.2e-75
Identity = 173/511 (33.86%), Postives = 282/511 (55.19%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHASKSL-PFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +D  V +   L PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 148 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 207
           + L++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 267
            Y   ++        S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 327
               + + +   +           R +  LR + + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 328 ICKVLSRDEFEMVNSKVFDKCENLVKQCLHDARVEIQEVSDVVVVGGCSYIPKIRKLMME 387
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++L+ +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + ++P EA   GAA++GA+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALILVYEGEETKAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGATLPGSQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004134140.12.2e-30993.51heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa ... [more]
XP_038897373.16.6e-30993.86heat shock 70 kDa protein 8 [Benincasa hispida][more]
KAA0049367.15.7e-30893.33heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70... [more]
XP_008438682.11.6e-30793.16PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo][more]
XP_022138266.11.7e-30492.63heat shock 70 kDa protein 8 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SKY82.1e-24471.95Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1[more]
Q9LHA82.2e-7634.25Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1[more]
P091895.3e-7534.56Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1[more]
Q9S9N11.5e-7434.24Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1[more]
P229535.9e-7433.86Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LAC61.1e-30993.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1[more]
A0A5D3D0522.7e-30893.33Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AXP08.0e-30893.16heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1[more]
A0A6J1C9928.2e-30592.63heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 ... [more]
A0A6J1GXD43.8e-30292.29heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT2G32120.11.5e-24571.95heat-shock protein 70T-2 [more]
AT2G32120.21.5e-24571.95heat-shock protein 70T-2 [more]
AT3G12580.11.5e-7734.25heat shock protein 70 [more]
AT1G16030.11.1e-7534.24heat shock protein 70B [more]
AT5G02500.14.2e-7533.86heat shock cognate protein 70-1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 167..187
score: 28.47
coord: 361..377
score: 45.28
coord: 502..518
score: 35.4
coord: 27..40
score: 41.3
coord: 393..413
score: 39.8
coord: 421..440
score: 36.79
NoneNo IPR availableGENE3D3.30.420.40coord: 212..390
e-value: 6.9E-79
score: 267.2
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 261..342
e-value: 6.9E-79
score: 267.2
NoneNo IPR availableGENE3D3.30.420.40coord: 29..408
e-value: 6.9E-79
score: 267.2
NoneNo IPR availableGENE3D3.30.30.30coord: 92..147
e-value: 6.9E-79
score: 267.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR19375:SF101HEAT SHOCK 70 KDA PROTEIN 8coord: 4..570
NoneNo IPR availableCDDcd10170HSP70_NBDcoord: 29..408
e-value: 3.84767E-78
score: 249.452
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 419..533
e-value: 4.8E-34
score: 119.5
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 423..525
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 28..525
e-value: 3.2E-89
score: 299.7
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 4..570
IPR018181Heat shock protein 70, conserved sitePROSITEPS01036HSP70_3coord: 364..378
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 27..209
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 224..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021268.1Lag0021268.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
biological_process GO:0009408 response to heat
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone
molecular_function GO:0051082 unfolded protein binding