Lag0021249 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0021249
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	N-acetyltransferase domain-containing protein
Location	chr7: 5884320 .. 5884832 (+)
RNA-Seq Expression	Lag0021249
Synteny	Lag0021249

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTCATCAAGAATTTCCGTCCGGCCGTTCAATCTCTCCGACGCCAACGACTTTCTCCGGTGGGCCGGCGACGACAGAGTAACCCGCTATCTCCGATGGAATACAATTGCCTCCAAAAATGAAGCATTAACTTACCTAGAGAAGGTCGCGATTCCCCATCCATGGCGGCGGTCCATCTGCTTGGACGGCCGTTCCGTCGGATACGTTTCGATCAAGCCGGAATCGGAGGAGAAATGCAGAGCGCATATCAGTTATGCTGTGGCAGCGGAGCATTGGGGCCGAGGAATAGCCACGGCGGCGCTGAGGGCGGCGATTCCAGCGGCGTTGAAAGAGTTTCCGGAGGTGGTTAGGGTTCAGGCGCTGGTGGAGGTCGAGAATGAGGGATCGCAGAGGGTTCTGGAGAAGGTGGGGTTTTGCAGAGAGGGGCAGTTGAGGAAATATGGAATTTGTAAGGGCGAAATTCGGGATTTGATAATTTTCAGCTTTTTAAGAACCGATCAACTCAAGTGA

mRNA sequence

ATGGACTCATCAAGAATTTCCGTCCGGCCGTTCAATCTCTCCGACGCCAACGACTTTCTCCGGTGGGCCGGCGACGACAGAGTAACCCGCTATCTCCGATGGAATACAATTGCCTCCAAAAATGAAGCATTAACTTACCTAGAGAAGGTCGCGATTCCCCATCCATGGCGGCGGTCCATCTGCTTGGACGGCCGTTCCGTCGGATACGTTTCGATCAAGCCGGAATCGGAGGAGAAATGCAGAGCGCATATCAGTTATGCTGTGGCAGCGGAGCATTGGGGCCGAGGAATAGCCACGGCGGCGCTGAGGGCGGCGATTCCAGCGGCGTTGAAAGAGTTTCCGGAGGTGGTTAGGGTTCAGGCGCTGGTGGAGGTCGAGAATGAGGGATCGCAGAGGGTTCTGGAGAAGGTGGGGTTTTGCAGAGAGGGGCAGTTGAGGAAATATGGAATTTGTAAGGGCGAAATTCGGGATTTGATAATTTTCAGCTTTTTAAGAACCGATCAACTCAAGTGA

Coding sequence (CDS)

ATGGACTCATCAAGAATTTCCGTCCGGCCGTTCAATCTCTCCGACGCCAACGACTTTCTCCGGTGGGCCGGCGACGACAGAGTAACCCGCTATCTCCGATGGAATACAATTGCCTCCAAAAATGAAGCATTAACTTACCTAGAGAAGGTCGCGATTCCCCATCCATGGCGGCGGTCCATCTGCTTGGACGGCCGTTCCGTCGGATACGTTTCGATCAAGCCGGAATCGGAGGAGAAATGCAGAGCGCATATCAGTTATGCTGTGGCAGCGGAGCATTGGGGCCGAGGAATAGCCACGGCGGCGCTGAGGGCGGCGATTCCAGCGGCGTTGAAAGAGTTTCCGGAGGTGGTTAGGGTTCAGGCGCTGGTGGAGGTCGAGAATGAGGGATCGCAGAGGGTTCTGGAGAAGGTGGGGTTTTGCAGAGAGGGGCAGTTGAGGAAATATGGAATTTGTAAGGGCGAAATTCGGGATTTGATAATTTTCAGCTTTTTAAGAACCGATCAACTCAAGTGA

Protein sequence

MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSICLDGRSVGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTDQLK

Homology

[Close]

BLAST of Lag0021249 vs. NCBI nr
Match: XP_023527193.1 (uncharacterized protein LOC111790505 [Cucurbita pepo subsp. pepo] >XP_023527195.1 uncharacterized protein LOC111790505 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.9 bits (803), Expect = 8.4e-82
Identity = 151/169 (89.35%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 1   MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSI 60
           M+SS ISVRPFNLSDA+DFLRWAGDDRVTRYLRWNTI SK EALTYLEKVAIPHPWRRSI
Sbjct: 17  MNSSTISVRPFNLSDADDFLRWAGDDRVTRYLRWNTITSKEEALTYLEKVAIPHPWRRSI 76

Query: 61  CLDGRSVGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQ 120
           CL+GRSVGYVS+KPESEEKCRA+ISYAVAAEHWGRGIATAALR AIPAAL +FPEVVR+Q
Sbjct: 77  CLNGRSVGYVSVKPESEEKCRANISYAVAAEHWGRGIATAALRVAIPAALIDFPEVVRIQ 136

Query: 121 ALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTDQL 170
           ALVEVENEGSQRVLEK+GFCREG LRKYG CKGEIR+ I+FSFLRTDQL
Sbjct: 137 ALVEVENEGSQRVLEKLGFCREGLLRKYGFCKGEIRNFIVFSFLRTDQL 185

[Close]

BLAST of Lag0021249 vs. NCBI nr
Match: KAA0049351.1 (putative N-acetyltransferase YoaA [Cucumis melo var. makuwa] >TYK17207.1 putative N-acetyltransferase YoaA [Cucumis melo var. makuwa])

HSP 1 Score: 312.4 bits (799), Expect = 2.4e-81
Identity = 147/169 (86.98%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 1   MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSI 60
           M+SSRIS+RPFNLSDA+DFLRWA D+RVTRYLRWNTI SK EALTY+EK+AIPH WRRSI
Sbjct: 1   MNSSRISIRPFNLSDADDFLRWASDERVTRYLRWNTITSKEEALTYIEKIAIPHQWRRSI 60

Query: 61  CLDGRSVGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQ 120
           CLDGRSVGYVS KPESEEKCRAHISYAVAAEHWG+GIAT ALRAAIPAAL+EFPEVVRVQ
Sbjct: 61  CLDGRSVGYVSFKPESEEKCRAHISYAVAAEHWGQGIATIALRAAIPAALREFPEVVRVQ 120

Query: 121 ALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTDQL 170
           A+VEVENEGSQ+VLEK+GFCREG LRKYG CKGEIRDL++FSFLRT+QL
Sbjct: 121 AMVEVENEGSQKVLEKLGFCREGVLRKYGFCKGEIRDLVVFSFLRTEQL 169

[Close]

BLAST of Lag0021249 vs. NCBI nr
Match: XP_022955367.1 (uncharacterized protein LOC111457416 [Cucurbita moschata])

HSP 1 Score: 312.0 bits (798), Expect = 3.2e-81
Identity = 150/169 (88.76%), Postives = 160/169 (94.67%), Query Frame = 0


Query: 1   MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSI 60
           M+SS ISVRPFNLSDA+DFLRWAGDDRVTRYLRWNTI SK EALTYLEKVAIPHPWRRSI
Sbjct: 1   MNSSTISVRPFNLSDADDFLRWAGDDRVTRYLRWNTITSKEEALTYLEKVAIPHPWRRSI 60

Query: 61  CLDGRSVGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQ 120
           CL+G SVGYVS+KPESEEKCR +ISYAVAAEHWGRGIATAALRAAIPAAL +FPEVVR+Q
Sbjct: 61  CLNGHSVGYVSVKPESEEKCRTNISYAVAAEHWGRGIATAALRAAIPAALIDFPEVVRIQ 120

Query: 121 ALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTDQL 170
           ALVEVENEGSQRVLEK+GFCREG LRKYG CKGEIR+ I+FSFLRTDQL
Sbjct: 121 ALVEVENEGSQRVLEKLGFCREGLLRKYGFCKGEIRNFIVFSFLRTDQL 169

[Close]

BLAST of Lag0021249 vs. NCBI nr
Match: KAE8650353.1 (hypothetical protein Csa_010832 [Cucumis sativus])

HSP 1 Score: 310.5 bits (794), Expect = 9.2e-81
Identity = 147/169 (86.98%), Postives = 160/169 (94.67%), Query Frame = 0


Query: 1   MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSI 60
           M+SSRIS+RPFNLSDA+DFLRWA D+RVTRYLRWNTI SK EALTYLEKVAIPH WRRSI
Sbjct: 1   MNSSRISIRPFNLSDADDFLRWASDERVTRYLRWNTITSKEEALTYLEKVAIPHQWRRSI 60

Query: 61  CLDGRSVGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQ 120
           CLDGRSVGYVS KPESEEKCRAHISYAVAAEHWG+GIAT ALRAAIPAAL++FPE+VRVQ
Sbjct: 61  CLDGRSVGYVSFKPESEEKCRAHISYAVAAEHWGQGIATIALRAAIPAALRQFPELVRVQ 120

Query: 121 ALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTDQL 170
           A+VEVENEGSQ+VLEK+GFCREG LRKYG CKGEIRDL++FS LRTDQL
Sbjct: 121 AMVEVENEGSQKVLEKLGFCREGVLRKYGFCKGEIRDLVVFSLLRTDQL 169

[Close]

BLAST of Lag0021249 vs. NCBI nr
Match: XP_004134439.1 (uncharacterized protein LOC101219695 [Cucumis sativus])

HSP 1 Score: 310.5 bits (794), Expect = 9.2e-81
Identity = 147/169 (86.98%), Postives = 160/169 (94.67%), Query Frame = 0


Query: 1   MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSI 60
           M+SSRIS+RPFNLSDA+DFLRWA D+RVTRYLRWNTI SK EALTYLEKVAIPH WRRSI
Sbjct: 1   MNSSRISIRPFNLSDADDFLRWASDERVTRYLRWNTITSKEEALTYLEKVAIPHQWRRSI 60

Query: 61  CLDGRSVGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQ 120
           CLDGRSVGYVS KPESEEKCRAHISYAVAAEHWG+GIAT ALRAAIPAAL++FPE+VRVQ
Sbjct: 61  CLDGRSVGYVSFKPESEEKCRAHISYAVAAEHWGQGIATIALRAAIPAALRQFPELVRVQ 120

Query: 121 ALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTDQL 170
           A+VEVENEGSQ+VLEK+GFCREG LRKYG CKGEIRDL++FS LRTDQL
Sbjct: 121 AMVEVENEGSQKVLEKLGFCREGVLRKYGFCKGEIRDLVVFSLLRTDQL 169

[Close]

BLAST of Lag0021249 vs. ExPASy Swiss-Prot
Match: P05332 (Uncharacterized N-acetyltransferase p20 OS=Bacillus licheniformis OX=1402 GN=p20 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.5e-12
Identity = 45/178 (25.28%), Postives = 82/178 (46.07%), Query Frame = 0


Query: 3   SSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSICL 62
           + R+++R   L DA+   ++  D  VT+Y+        ++A   ++ +         + L
Sbjct: 6   TERLTLRKMELEDADVLCQYWSDPEVTKYMNITPFTDVSQARDMIQMI-------NDLSL 65

Query: 63  DGRS-------------VGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAA 122
           +G++             +G        +E  RA I Y +   HWG+G A+ A++  I   
Sbjct: 66  EGQANRFSIIVKETDEVIGTCGFNMIDQENGRAEIGYDLGRNHWGKGFASEAVQKLIDYG 125

Query: 123 LKEFPEVVRVQALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTD 168
                 + R++A VE EN  S ++L  + F +EG LR Y   KG + D+ +FS L+ +
Sbjct: 126 FTSL-NLNRIEAKVEPENTPSIKLLNSLSFQKEGLLRDYEKAKGRLIDVYMFSLLKRE 175

[Close]

BLAST of Lag0021249 vs. ExPASy Swiss-Prot
Match: O34569 (Uncharacterized N-acetyltransferase YoaA OS=Bacillus subtilis (strain 168) OX=224308 GN=yoaA PE=3 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-10
Identity = 44/171 (25.73%), Postives = 81/171 (47.37%), Query Frame = 0


Query: 1   MDSSRISVRPFNLSDANDFLRWAGDDRVTRYLRWNTIASKNEALTYLEKVAIPHPWRRSI 60
           +++ R+ +R     DA        +D VTRY     + S  +A++ ++  A  +  +R I
Sbjct: 5   LETDRLILRQITDQDAEAIFACFSNDEVTRYYGLENMESIEQAISMIQTFAALYQEKRGI 64

Query: 61  --CLDGRS----VGYVSIKPESEEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFP 120
              ++ R     +G +     +++  RA I Y +  EHW  G A+  +   +        
Sbjct: 65  RWGIERRDTKELIGTIGFHALAQKHRRAEIGYEIIPEHWRNGFASEVISKVVSYGFSAL- 124

Query: 121 EVVRVQALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLR 166
            + R+ A+V  +NE S R+L K+GF +EG LR+Y    G   D  ++S ++
Sbjct: 125 GLSRIGAVVFTDNEASNRLLLKMGFQKEGVLRQYMYQNGTPYDTNVYSIVK 174

[Close]

BLAST of Lag0021249 vs. ExPASy Swiss-Prot
Match: O31633 (Putative [ribosomal protein S5]-alanine N-acetyltransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=yjcK PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 52/173 (30.06%), Postives = 80/173 (46.24%), Query Frame = 0


Query: 6   ISVRPFNLSDANDFLRWAGDDR-------VTRYLRWNTIASKNEALT-YLEKVAIPHPWR 65
           I VRP  ++DA + L    ++R       + R   + T+  + + +T Y E++     + 
Sbjct: 7   IYVRPLEVTDAEENLGLQSENRDFFEQFSMIRADDYYTVEGQRKRITEYQERLEKDEEYH 66

Query: 66  RSI--CLDGRSVGYVSIKPESEEKCR-AHISYAVAAEHWGRGIATAALRAAIPAALKEFP 125
             I    D R +G VS+        + A I Y +   H G+GI T A+R  +  A  E  
Sbjct: 67  FGIFTASDDRLIGTVSLFQIIRGALQTAFIGYFLDKAHNGKGIMTEAVRLVVDYAFHEL- 126

Query: 126 EVVRVQALVEVENEGSQRVLEKVGFCREGQLRKYGICKGEIRDLIIFSFLRTD 168
           ++ R++A V   N GS RVLEK GF +EG  RK     G   D  + + L  D
Sbjct: 127 KLHRIEAGVMPRNLGSMRVLEKAGFHKEGIARKNVKINGVWEDHQVLAILNPD 178

[Close]

BLAST of Lag0021249 vs. ExPASy Swiss-Prot
Match: P49855 (Uncharacterized protein YkkB OS=Bacillus subtilis (strain 168) OX=224308 GN=ykkB PE=4 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.5e-04
Identity = 39/140 (27.86%), Postives = 57/140 (40.71%), Query Frame = 0


Query: 11  FNLSDANDFLRWAGDDRVTR-YLRWNTIASKNEALTYLEKVAIPHPWRRSICLDGRSVGY 70
           FN  D   +     D R TR ++ WN    K   ++          W       G  +G 
Sbjct: 30  FNDPDVMTYYSGLKDKRQTREWVNWNQRNEKGYGVSL---------WIAEDKRTGEFLGQ 89

Query: 71  VSIKPES-EEKCRAHISYAVAAEHWGRGIATAALRAAIPAALKEFPEVVRVQALVEVENE 130
             I P+  E +    I Y  A  HWG G A  A RA +     E  +  ++ AL++  N+
Sbjct: 90  CGIVPQQIENQTVMEIGYMFARRHWGNGYAQEAARACLDYGFNE-RQFGKMAALIDPGNK 149

Query: 131 GSQRVLEKVGFCREGQLRKY 149
            S RV EK+G      +RK+
Sbjct: 150 ASIRVAEKIGMHYSKTIRKW 159

The following BLAST results are available for this feature:

BLAST of Lag0021249 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527193.1	8.4e-82	89.35	uncharacterized protein LOC111790505 [Cucurbita pepo subsp. pepo] >XP_023527195....	[more]
KAA0049351.1	2.4e-81	86.98	putative N-acetyltransferase YoaA [Cucumis melo var. makuwa] >TYK17207.1 putativ...	[more]
XP_022955367.1	3.2e-81	88.76	uncharacterized protein LOC111457416 [Cucurbita moschata]	[more]
KAE8650353.1	9.2e-81	86.98	hypothetical protein Csa_010832 [Cucumis sativus]	[more]
XP_004134439.1	9.2e-81	86.98	uncharacterized protein LOC101219695 [Cucumis sativus]	[more]

BLAST of Lag0021249 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05332	9.5e-12	25.28	Uncharacterized N-acetyltransferase p20 OS=Bacillus licheniformis OX=1402 GN=p20...	[more]
O34569	1.0e-10	25.73	Uncharacterized N-acetyltransferase YoaA OS=Bacillus subtilis (strain 168) OX=22...	[more]
O31633	2.4e-07	30.06	Putative [ribosomal protein S5]-alanine N-acetyltransferase OS=Bacillus subtilis...	[more]
P49855	1.5e-04	27.86	Uncharacterized protein YkkB OS=Bacillus subtilis (strain 168) OX=224308 GN=ykkB...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000182	GNAT domain	PFAM	PF13302	Acetyltransf_3	coord: 5..139 e-value: 5.0E-23 score: 82.3
IPR000182	GNAT domain	PROSITE	PS51186	GNAT	coord: 6..165 score: 16.568041
None	No IPR available	GENE3D	3.40.630.30		coord: 1..170 e-value: 4.0E-40 score: 139.5
None	No IPR available	PANTHER	PTHR46067:SF18	ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN	coord: 1..167
None	No IPR available	PANTHER	PTHR46067	ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN	coord: 1..167
None	No IPR available	CDD	cd04301	NAT_SF	coord: 60..99 e-value: 0.00164365 score: 33.4033
IPR016181	Acyl-CoA N-acyltransferase	SUPERFAMILY	55729	Acyl-CoA N-acyltransferases (Nat)	coord: 3..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0021249.1	Lag0021249.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008080	N-acetyltransferase activity