Lag0021190 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021190
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr7: 5407655 .. 5410092 (+)
RNA-Seq ExpressionLag0021190
SyntenyLag0021190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAGGCATATGATAGAGTCGAATGGGTTTTCTTGGAGAAGATTATGCTGAAAATGGGTTTTGCTCCGGAGTGGGTGGATCTGATTTCTCTTTGTATTTCTTCCGTACGTTTCTCTTTTAATGTGAATGGGATCAGGTGTGGGGGTGTGGTTCCGAGTAGAGGTTTGCGGCAGGGTGACCCATTATCCCCGTATCTTTTTCTTTATGTGCTGAGGGGTTGTCATGTATGCTTCGGGGGGCGGAGGAGTCGAAGGCAATTAGGGGATTGAAGGTAGCTCGGTATGGTCCTGCTATCTCACATTTATTTTTCGCTGACGATAGTCTGCTATTTTTCAGGGCCAACGAGAGTGATGCCACAGCGGTTCGGGGCATTCTGGACTGTTATGAACGGGTGTCGGGTCAGACAGTGAATTTCGATAAGTCTATTATCTCTTTCAGTCCGAGTACGGATGGGAGGGTTAAGGCTCAGGTGGGTCAGATTCTGCAGGTTCAGGTTACGGCATGGCACCGCCAATATCTAGGCCTACCTTCTTTTATGCCACGGGACAGAACGAGCTCGTTGAGTTTCATTAAGGATCGAGTATGGCAGCAGATTCAGGGGTGGAAGGGCAAACTATTCTCAGTTGGGGGTAGGGAGGTTCTTCTAAAGTCTGTTGTGCAGGCGATTCCGTGTTATTCGATGAATTGCTTCCGTTTACCAAAGAAGTTGATTCTTGAGATCAGCAGAGCCATGGCCCGGTTTTGGTGGGGTGGGGAGAAGGTGGATCGAGGAATTCATTGGGTGAGTTGGAAATCCCTATGTAAGCCTAAGTGCTATGGTGGGATGGGGTTTAGGGATATGGAGATTTTTAACCAAGCTTTGCTGGCAAAACAGTGTTGGAGAATTATCCAGAATCCATCTTCCCTCCTGGCACGTGTTCTGAAGGGCAGATATTTTCCTTTCGGAGATTTCTTGAGGGCAAATGTGGGGACGAGACCGTCTTTTATATGGAGGAGTTTATTGTGGGGAAGAGAGCTTTTGGAGATGGGCTTGCGTTGGCAAGTGGGGAATGGAGAAAGTGTGTCGGTATATGGAGTGAATTGGCTTCCTGATGATGGTAATTTTAGAGTACGGTCATCAGGGACTTTGGCTCCGGAGACTCGGGTTGCAGACCTGATGACGGCATCGGGGCAGTGGAATGAAGGGCTCATCCGACAGAATTTTAGCCCTCAGGAGGTCGGTCTAATTTTGTCAATTCTTGTGCGGGCTGGGGCGGAGGATAAGATTGTTTGGCATTATGAGAAGTCGGGCCTGTTTTCGGTGAAAAGCGGATATCGGTTGGGGCAATCAACCTGGCTTGCGCAGTTTCCGTCTTCCTCTTCGAACGAGTCGGCAATGGGTTGGTGGAAGGGGGTTTGGAAAATGTTTATTCCGAATAAGATCAAGATTTTTCTTTGGAGACTTTGTTTAGACCGCTTGCCCACGATTGATAATTTGGGTGTTCGGGGCTGTGACGTGTTGAACGTTTGTGGCCTCTGTAGGCGAGGTGGGGAGTCCAGTCTGCATGTCTTCTGGCATTGCAAGTTCGTGAAGGCCGTGTTGATGGAGTCCGAGTTTGGAGGATTGATACATAATGTGCAGGCTGGGTCTATGTTTGATCTGCTTAGGGAAGTGAAGGACAAGGTTGGTTGGGGAAGATTTGAGTTGTTTGTGGTGGTGCTGTGGGCTGTGTGGAACTGCCGAAATCAGCGGAAATTTAGGGGCCTAGAACCAGTGGTTGGGCTAGTAGAATGGGCGACGAGTTATATCTCGTCGTTCCAGCAGGCGACCTCGGCCTGTGGTGTTGGGTGAGGAGTGTTCTTGCAAGGGAAGATGTGAGATGGAGCCCCCCGGAGGCTGGGTGGTATAAGGTGAACGTAGATGCGTCCTTTAGGAGGGAGCGATGGCAGGCGGGTCTGGGAGTGGTGGTTCGGGATTCCTCTGGTCGGGTTATGTTGTCGGCATCTTTGGTGCAGCGACATGTGCGAAGCCCGGAGATGGCTGAAGGATGGGCCGCAGTTAAGGGAACGAGACTGGCAGTGGAGATGGGTTTGGGCCCATTGATGTTGGAGACTGACTCCAGTCGGGTGGCTAGCTTTTTCCAAGATGAGGCAGGGGATGACTTCTCAGATGTGGGTGCCCTGGTGGCTGAATTACGAAGGGACATGCCAGATCCTTCCTTTTTCGGTTGCAGGTTCACTCGAAGGGAGGGAAATGAGGTGGCACACCAGTTGGCTTTTCTGGCAGGGAGGGACGAAGTGTCTAGGGTGTGGGTTGAGTTTGTACCCCAGTGTGTTGAGGCGTTGATCCTTTCTGATATGGCACTGTTGTGATGTTTTCTTACAGGTTACGACGCGCAGGCATGATGCTGGTGAAGATCGTGAATGGATAGTGAACAAAGAGCCATCAAATTAG

mRNA sequence

ATGAGTAAGGCATATGATAGAGTCGAATGGGTTTTCTTGGAGAAGATTATGCTGAAAATGGGTTTTGCTCCGGAGTGGGTGGATCTGATTTCTCTTTGTATTTCTTCCGTACGTTTCTCTTTTAATGTGAATGGGATCAGGTGTGGGGGTGTGGTTCCGAGTAGAGGTTTGCGGCAGGGTGACCCATTATCCCCGGCCAACGAGAGTGATGCCACAGCGGTTCGGGGCATTCTGGACTGTTATGAACGGGTGTCGGGTCAGACAGTGAATTTCGATAAGTCTATTATCTCTTTCAGTCCGAGTACGGATGGGAGGGTTAAGGCTCAGGTGGGTCAGATTCTGCAGGTTCAGGTTACGGCATGGCACCGCCAATATCTAGGCCTACCTTCTTTTATGCCACGGGACAGAACGAGCTCGTTGAGTTTCATTAAGGATCGAGTATGGCAGCAGATTCAGGGGTGGAAGGGCAAACTATTCTCAGTTGGGGGTAGGGAGGTTCTTCTAAAGTCTGTTGTGCAGGCGATTCCGTGTTATTCGATGAATTGCTTCCGTTTACCAAAGAAGTTGATTCTTGAGATCAGCAGAGCCATGGCCCGGTTTTGGTGGGGTGGGGAGAAGGTGGATCGAGGAATTCATTGGGTGAGTTGGAAATCCCTATGTAAGCCTAAGTGCTATGGTGGGATGGGGTTTAGGGATATGGAGATTTTTAACCAAGCTTTGCTGGCAAAACAGTGTTGGAGAATTATCCAGAATCCATCTTCCCTCCTGGCACGTGTTCTGAAGGGCAGATATTTTCCTTTCGGAGATTTCTTGAGGGCAAATGTGGGGACGAGACCGTCTTTTATATGGAGGAGTTTATTGTGGGGAAGAGAGCTTTTGGAGATGGGCTTGCGTTGGCAAGTGGGGAATGGAGAAAGTGTGTCGGTATATGGAGTGAATTGGCTTCCTGATGATGGTAATTTTAGAGTACGGTCATCAGGGACTTTGGCTCCGGAGACTCGGGTTGCAGACCTGATGACGGCATCGGGGCAGTGGAATGAAGGGCTCATCCGACAGAATTTTAGCCCTCAGGAGGTCGGTCTAATTTTGTCAATTCTTGTGCGGGCTGGGGCGGAGGATAAGATTGTTTGGCATTATGAGAAGTCGGGCCTGTTTTCGGTGAAAAGCGGATATCGGTTGGGGCAATCAACCTGGCTTGCGCAGTTTCCGTCTTCCTCTTCGAACGAGTCGGCAATGGGTTGGTGGAAGGGGGTTTGGAAAATGTTTATTCCGAATAAGATCAAGATTTTTCTTTGGAGACTTTGTTTAGACCGCTTGCCCACGATTGATAATTTGGGTGTTCGGGGCTGTGACGTGTTGAACGTTTGTGGCCTCTGTAGGCGAGGTGGGGAGTCCAGTCTGCATGTCTTCTGGCATTGCAAGTTCGTGAAGGCCGTGTTGATGGAGTCCGAGTTTGGAGGATTGATACATAATGTGCAGGCTGGGTCTATGTTTGATCTGCTTAGGGAAGTGAAGGACAAGGGGCCTAGAACCAGTGGTTGGGCTAGTAGAATGGGCGACGAGTTATATCTCGTCGTTCCAGCAGGCGACCTCGGCCTGTGGTGTTGGGTGAGGAGTGTTCTTGCAAGGGAAGATGTGAGATGGAGCCCCCCGGAGGCTGGGTGGTATAAGGTGAACGTAGATGCGTCCTTTAGGAGGGAGCGATGGCAGGCGGGTCTGGGAGTGGTGGTTCGGGATTCCTCTGGTCGGGTTATGTTGTCGGCATCTTTGGTGCAGCGACATGTGCGAAGCCCGGAGATGGCTGAAGGATGGGCCGCAGTTAAGGGAACGAGACTGGCAGTGGAGATGGGTTTGGGCCCATTGATGTTGGAGACTGACTCCAGTCGGGTGGCTAGCTTTTTCCAAGATGAGGCAGGGGATGACTTCTCAGATGTGGGTGCCCTGGTGGCTGAATTACGAAGGGACATGCCAGATCCTTCCTTTTTCGGTTGCAGGTTCACTCGAAGGGAGGGAAATGAGGTGGCACACCAGTTGGCTTTTCTGGCAGGGAGGGACGAAGTGTCTAGGGTTACGACGCGCAGGCATGATGCTGGTGAAGATCGTGAATGGATAGTGAACAAAGAGCCATCAAATTAG

Coding sequence (CDS)

ATGAGTAAGGCATATGATAGAGTCGAATGGGTTTTCTTGGAGAAGATTATGCTGAAAATGGGTTTTGCTCCGGAGTGGGTGGATCTGATTTCTCTTTGTATTTCTTCCGTACGTTTCTCTTTTAATGTGAATGGGATCAGGTGTGGGGGTGTGGTTCCGAGTAGAGGTTTGCGGCAGGGTGACCCATTATCCCCGGCCAACGAGAGTGATGCCACAGCGGTTCGGGGCATTCTGGACTGTTATGAACGGGTGTCGGGTCAGACAGTGAATTTCGATAAGTCTATTATCTCTTTCAGTCCGAGTACGGATGGGAGGGTTAAGGCTCAGGTGGGTCAGATTCTGCAGGTTCAGGTTACGGCATGGCACCGCCAATATCTAGGCCTACCTTCTTTTATGCCACGGGACAGAACGAGCTCGTTGAGTTTCATTAAGGATCGAGTATGGCAGCAGATTCAGGGGTGGAAGGGCAAACTATTCTCAGTTGGGGGTAGGGAGGTTCTTCTAAAGTCTGTTGTGCAGGCGATTCCGTGTTATTCGATGAATTGCTTCCGTTTACCAAAGAAGTTGATTCTTGAGATCAGCAGAGCCATGGCCCGGTTTTGGTGGGGTGGGGAGAAGGTGGATCGAGGAATTCATTGGGTGAGTTGGAAATCCCTATGTAAGCCTAAGTGCTATGGTGGGATGGGGTTTAGGGATATGGAGATTTTTAACCAAGCTTTGCTGGCAAAACAGTGTTGGAGAATTATCCAGAATCCATCTTCCCTCCTGGCACGTGTTCTGAAGGGCAGATATTTTCCTTTCGGAGATTTCTTGAGGGCAAATGTGGGGACGAGACCGTCTTTTATATGGAGGAGTTTATTGTGGGGAAGAGAGCTTTTGGAGATGGGCTTGCGTTGGCAAGTGGGGAATGGAGAAAGTGTGTCGGTATATGGAGTGAATTGGCTTCCTGATGATGGTAATTTTAGAGTACGGTCATCAGGGACTTTGGCTCCGGAGACTCGGGTTGCAGACCTGATGACGGCATCGGGGCAGTGGAATGAAGGGCTCATCCGACAGAATTTTAGCCCTCAGGAGGTCGGTCTAATTTTGTCAATTCTTGTGCGGGCTGGGGCGGAGGATAAGATTGTTTGGCATTATGAGAAGTCGGGCCTGTTTTCGGTGAAAAGCGGATATCGGTTGGGGCAATCAACCTGGCTTGCGCAGTTTCCGTCTTCCTCTTCGAACGAGTCGGCAATGGGTTGGTGGAAGGGGGTTTGGAAAATGTTTATTCCGAATAAGATCAAGATTTTTCTTTGGAGACTTTGTTTAGACCGCTTGCCCACGATTGATAATTTGGGTGTTCGGGGCTGTGACGTGTTGAACGTTTGTGGCCTCTGTAGGCGAGGTGGGGAGTCCAGTCTGCATGTCTTCTGGCATTGCAAGTTCGTGAAGGCCGTGTTGATGGAGTCCGAGTTTGGAGGATTGATACATAATGTGCAGGCTGGGTCTATGTTTGATCTGCTTAGGGAAGTGAAGGACAAGGGGCCTAGAACCAGTGGTTGGGCTAGTAGAATGGGCGACGAGTTATATCTCGTCGTTCCAGCAGGCGACCTCGGCCTGTGGTGTTGGGTGAGGAGTGTTCTTGCAAGGGAAGATGTGAGATGGAGCCCCCCGGAGGCTGGGTGGTATAAGGTGAACGTAGATGCGTCCTTTAGGAGGGAGCGATGGCAGGCGGGTCTGGGAGTGGTGGTTCGGGATTCCTCTGGTCGGGTTATGTTGTCGGCATCTTTGGTGCAGCGACATGTGCGAAGCCCGGAGATGGCTGAAGGATGGGCCGCAGTTAAGGGAACGAGACTGGCAGTGGAGATGGGTTTGGGCCCATTGATGTTGGAGACTGACTCCAGTCGGGTGGCTAGCTTTTTCCAAGATGAGGCAGGGGATGACTTCTCAGATGTGGGTGCCCTGGTGGCTGAATTACGAAGGGACATGCCAGATCCTTCCTTTTTCGGTTGCAGGTTCACTCGAAGGGAGGGAAATGAGGTGGCACACCAGTTGGCTTTTCTGGCAGGGAGGGACGAAGTGTCTAGGGTTACGACGCGCAGGCATGATGCTGGTGAAGATCGTGAATGGATAGTGAACAAAGAGCCATCAAATTAG

Protein sequence

MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQGDPLSPANESDATAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSFMPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYGVNWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSILVRAGAEDKIVWHYEKSGLFSVKSGYRLGQSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGLIHNVQAGSMFDLLREVKDKGPRTSGWASRMGDELYLVVPAGDLGLWCWVRSVLAREDVRWSPPEAGWYKVNVDASFRRERWQAGLGVVVRDSSGRVMLSASLVQRHVRSPEMAEGWAAVKGTRLAVEMGLGPLMLETDSSRVASFFQDEAGDDFSDVGALVAELRRDMPDPSFFGCRFTRREGNEVAHQLAFLAGRDEVSRVTTRRHDAGEDREWIVNKEPSN
Homology
BLAST of Lag0021190 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 569.7 bits (1467), Expect = 3.5e-158
Identity = 305/722 (42.24%), Postives = 418/722 (57.89%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLC-ISSVRFSFNVNGIRCGG--------- 60
            MSKAYDRVEW FLE +MLKMGF   W    + C +  +         R G          
Sbjct: 426  MSKAYDRVEWNFLEAVMLKMGFDFRWKVYQTYCNVLQIERVLRDYVSRVGALLLLTFSLL 485

Query: 61   VVPSRGLR-QGDPLSPANESDATAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQ 120
            ++ S  LR  G  +S A  +           Y + +GQ      ++I   P    R  + 
Sbjct: 486  MIVSYFLRLVGGKVSVAEIA-----------YRKCTGQ------ALIKL-PEYQDRQSSL 545

Query: 121  VGQILQVQVTAWHRQYLGLPSFMPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLK 180
            +  IL V +     QYLGLP+FMPR+R    ++IKDRVW+ +QGWK KLFS+GG+EVL+K
Sbjct: 546  IQNILSVNMVECQLQYLGLPTFMPRNRRMHFNYIKDRVWKHLQGWKAKLFSIGGKEVLIK 605

Query: 181  SVVQAIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMG 240
            +V QAIPCY+M+CFRLPK+LI E     ARFWWG  K D+ IHWV+W SL  PKC GGMG
Sbjct: 606  AVAQAIPCYTMSCFRLPKRLIREFHHITARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMG 665

Query: 241  FRDMEIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWG 300
            FRD+E+FN+ALLAKQCWRI+ +P+S+L+RVLKGRYF    F+ A +   PS+IWRS+LWG
Sbjct: 666  FRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWG 725

Query: 301  RELLEMGLRWQVGNGESVSVYGVNWLPDDGNFRVRSSGTLAPETRVADLMT-ASGQWNEG 360
            R+LL+ GLRW++GNG+SV +YG NW+P+    ++ SS  L   +RV+ L+    G W   
Sbjct: 726  RDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGD 785

Query: 361  LIRQNFSPQEVGLILSILVRAGA-EDKIVWHYEKSGLFSVKSGYRLG-QSTWLAQFPSSS 420
            ++R  F+P E   ILSI +  GA ED+++W+YEK+G++SV+SGY++   +    Q PSSS
Sbjct: 786  VVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSS 845

Query: 421  SNESAMGWWKGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESS 480
            S+E    WW G WKM IPNKIK+FLWRLCLDRLPT  NL  RG ++ N C  C R GE S
Sbjct: 846  SSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDS 905

Query: 481  LHVFWHCKFVKAVLMESEFGGLIHNVQAGSMFDLLREVKDKGPRTSG----------WAS 540
            +H+FW CKF +A+ + S+FG L       S F +LRE  +   +             W  
Sbjct: 906  IHLFWICKFAEALWINSKFGKL-------SPFLILRESHESLSKADFEELCVVIWGLWNQ 965

Query: 541  R----MGDELYLVVPAG-DLGLWCWVRSVLARE--------------DVRWSPPEAGWYK 600
            R      D    V   G +L  W    ++  RE              ++ W PP+ G YK
Sbjct: 966  RNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAEILWQPPDEGIYK 1025

Query: 601  VNVDASFRRERWQAGLGVVVRDSSGRVMLSASLVQRHVRSPEMAEGWAAVKGTRLAVEMG 660
            +N DASF      AGLG+++ +  G+VM +A+    +++S +MAE  AAV+G +LA E+G
Sbjct: 1026 INTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIG 1085

Query: 661  LGPLMLETDSSRVASFFQDEAGDDFSDVGALVAELRRDMPDPSFFGCRFTRREGNEVAHQ 680
            + P +                 +D S+ G +V + +            F +REGN+ AH 
Sbjct: 1086 MHPAL-----------------EDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHM 1105

BLAST of Lag0021190 vs. NCBI nr
Match: VVA32947.1 (PREDICTED: retrotransposon [Prunus dulcis])

HSP 1 Score: 509.2 bits (1310), Expect = 5.7e-140
Identity = 293/766 (38.25%), Postives = 392/766 (51.17%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
            M+KAYDRVEWVFL ++MLK+GF+  WV  +  CIS+  FS    G   G ++P RGLRQG
Sbjct: 509  MAKAYDRVEWVFLREMMLKLGFSATWVAKVMDCISTTTFSMLWKGNPVGHIMPQRGLRQG 568

Query: 61   DPLSP-------------------------------------------------ANESDA 120
             PLSP                                                 A     
Sbjct: 569  CPLSPYLFLMCTEGFSCLLRGAERRGDLVGVQVARGGPSVTHLLFADDSILFMKATNEAC 628

Query: 121  TAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSF 180
             A+  +   YE VSGQ +N+ KS  S SP+        +  +L V V   H +YLGLP+ 
Sbjct: 629  RALETLFQTYEEVSGQQINYSKSAFSLSPNATRADFDMIKGVLNVPVVQCHEKYLGLPTI 688

Query: 181  MPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLIL 240
              + R      +KD++W+ I GWK KL S  G+E+L+K+V+QAIP YSM+CFR+PK L  
Sbjct: 689  AGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILMKAVLQAIPTYSMSCFRIPKGLCK 748

Query: 241  EISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQN 300
            E++  MARFWW   K  RGIHWV W+ LCK K  GG+GFRD+E FNQALLAKQCWRI++ 
Sbjct: 749  ELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRT 808

Query: 301  PSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYG 360
            P SL+AR+ + RY P   FL A VGT PSFIWRSL WG+ELL  GLRW+VGNG S+ VY 
Sbjct: 809  PESLVARIFRARYHPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGNGVSIQVYT 868

Query: 361  VNWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI-LVRAG 420
              WLP    F++ S   L   T V DL T+SGQWN  L++  F  QEV   L I L    
Sbjct: 869  DKWLPAPSFFKIMSPPQLPLSTLVCDLFTSSGQWNVPLLKDIFWDQEVDAKLQIPLASLA 928

Query: 421  AEDKIVWHYEKSGLFSVKSGYRLG--QSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIK 480
              D ++WHYE++G++SVKSGYRL   +   ++  PS   + ++  +WK +W + IPNKIK
Sbjct: 929  GHDCLIWHYERNGMYSVKSGYRLACLEKDKMSGEPSVRVDLNSK-FWKKIWALKIPNKIK 988

Query: 481  IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGL 540
             FLWR   D LP    L  R      +C  C R  ES LH  W C+  K V   S +G +
Sbjct: 989  FFLWRCAWDFLPCGQILFNRKIAPTPICPNCHRKAESVLHAVWLCETAKEVWRNSAWGNV 1048

Query: 541  IHNVQAGSMFDLLREV----------------------------KDKGPRTSGWASRMGD 600
                +  S  +L   +                            + K    +    RM  
Sbjct: 1049 CEEWRVNSFRELWHALQLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETATQLLHRMTK 1108

Query: 601  ELYLVVPAGDLGLWCWVRSVLAREDVR-WSPPEAGWYKVNVDASFRRERWQAGLGVVVRD 660
                   A +L      R    +  +  W PP AG YK+NVD + +      G+GVVVR+
Sbjct: 1109 LAQEFSNANNLSHTIHGRQSSPQAPLHGWRPPPAGIYKINVDGAVKSGDSVRGVGVVVRN 1168

Query: 661  SSGRVMLSASLVQRHVRS--PEMAEGWAAVKGTRLAVEMGLGPLMLETDSSR-VASFFQD 683
            ++G  M  A+ V+R   S      E  A ++G R A++MG    +LE D+   + S    
Sbjct: 1169 ANGEFM--AACVRRIQASYGARQTELMATIEGLRFAIDMGFTAAVLEMDAQDCINSILST 1228

BLAST of Lag0021190 vs. NCBI nr
Match: ONI01138.1 (hypothetical protein PRUPE_6G123900 [Prunus persica])

HSP 1 Score: 496.5 bits (1277), Expect = 3.8e-136
Identity = 295/769 (38.36%), Postives = 391/769 (50.85%), Query Frame = 0

Query: 1   MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
           M+KAYDRVEWVFL  +MLK+GF+  WV  +  CIS+  FS    G   G ++P RGLRQG
Sbjct: 243 MAKAYDRVEWVFLRAMMLKLGFSATWVSKVMDCISTTTFSVLWKGTPVGHIMPQRGLRQG 302

Query: 61  DPLSP-------------------------------------------------ANESDA 120
            PLSP                                                 A   D 
Sbjct: 303 CPLSPYLFLICTEGFSCLLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKDC 362

Query: 121 TAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSF 180
            A+  +   YE V+GQ +N+ KS +S SP+        +  +L V V   H  YLGLP+ 
Sbjct: 363 MALETLFQTYEEVTGQQINYSKSALSLSPNATRADFDMIEGVLNVPVVRCHENYLGLPTI 422

Query: 181 MPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLIL 240
             + R      +KD++W+ I GWK KL S  G+E+L+K+V+QAIP YSM+CFR+PK L  
Sbjct: 423 AGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFRIPKGLCK 482

Query: 241 EISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQN 300
           E++  MARFWW   K  RGIHWV W+ LCK K  GG+GFRD+E FNQALLAKQCWRI++ 
Sbjct: 483 ELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRT 542

Query: 301 PSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYG 360
           P SL+AR+ + RY P   FL A VGT PSFIWRSL WG+ELL  GLRW+VG+G S+ VY 
Sbjct: 543 PESLVARIFRARYHPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGSGVSIQVYT 602

Query: 361 VNWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI-LVRAG 420
             WLP    F++ S   L   TRV DL T+SGQWN  L++  F  QEV  IL I L    
Sbjct: 603 DKWLPAPSCFKIMSPPQLPLSTRVCDLFTSSGQWNVPLLKDIFWDQEVDAILQIPLASLA 662

Query: 421 AEDKIVWHYEKSGLFSVKSGYRLG--QSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIK 480
             D ++WHYE++G++SVKSGYRL   +   ++  PS+  + ++  +WK +W + IPNKIK
Sbjct: 663 GHDCLIWHYERNGMYSVKSGYRLAGLEKDKMSGEPSARVDLNSK-FWKKIWALKIPNKIK 722

Query: 481 IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGL 540
            FLWR   D LP    L  R      +C  C R  ES LH  W C+  K V   S +G +
Sbjct: 723 FFLWRCAWDFLPCGQILFNRKIAPTPICPKCHRKAESVLHAVWLCEAAKEVWRNSAWGNV 782

Query: 541 IHNVQAGSMFDLLREVKDKGPRTSGWASRMGDELYLVVPAGDLGLW-------------- 600
               +  S  +L   +     + S      G   YL       GLW              
Sbjct: 783 CEVWRVNSFRELWHAL-----QLSSSGEEQGLFAYLC-----WGLWNRRNSFIFEGKSET 842

Query: 601 ---------------CWVRSVLAREDVRWSPPEA---GWYKVNVDASFRRERWQAGLGVV 660
                              ++L     R S P+A   GW       S    R   G+GVV
Sbjct: 843 AIQLLSRMTKLAQEFSDANNILHTIHGRQSSPQAPLQGWRPPPAVKSGDSVR---GVGVV 902

Query: 661 VRDSSGRVMLSASLVQRHVRS--PEMAEGWAAVKGTRLAVEMGLGPLMLETDSSR-VASF 683
           VR+++G  M  A+ V+R   S      E  A ++G R A++MG    +LE D+   + S 
Sbjct: 903 VRNANGEFM--AACVRRIHASYGARQTELMATIEGLRFAIDMGFTDAILEMDAQDCLNSI 962

BLAST of Lag0021190 vs. NCBI nr
Match: XP_024956542.1 (uncharacterized protein LOC112498908 [Citrus sinensis])

HSP 1 Score: 475.3 bits (1222), Expect = 9.1e-130
Identity = 266/759 (35.05%), Postives = 390/759 (51.38%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
            +SKAYDR+EW FLE+IM ++GF+ +W+ LI  CISSV FS  +NG   G + P RGLRQG
Sbjct: 348  VSKAYDRLEWSFLEQIMKRLGFSHQWISLIMRCISSVSFSVIINGSVKGFIKPQRGLRQG 407

Query: 61   DPLSP------------------------------------------------ANESDAT 120
             P+SP                                                A+ +D  
Sbjct: 408  CPISPYLFITCAEAFSSLLQQAEQQRLIHGLSFGKELKISHLLFADDSLIFCRASVADCQ 467

Query: 121  AVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSFM 180
             ++ ILDCY   SGQ  NF+KS +  S +      + +G I  + + + +  YLGLP+ +
Sbjct: 468  KLKMILDCYSLASGQIFNFEKSSLFLSGNVQQAQASAIGNIFHLNIVSKYENYLGLPAMV 527

Query: 181  PRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLILE 240
             R R+   + IK +V  +I  W+ K FS GG+EVL+K+ VQAIP ++M+ F++P  +  +
Sbjct: 528  GRKRSGFFNDIKLKVLNKISSWQHKFFSCGGKEVLIKAAVQAIPAFAMSVFKIPLGICED 587

Query: 241  ISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQNP 300
            I R +  FWWG  +  R IHW  W+ + + KC GGMGFRD   FNQALLAKQ WRI Q P
Sbjct: 588  IQRIVGNFWWGSSRERRSIHWSKWEKMSQAKCKGGMGFRDFASFNQALLAKQGWRIQQCP 647

Query: 301  SSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYGV 360
             SL+ARVL+ RYF   +FL A +G+ PS+IWRS+LWGR+++  G RW++GNG+ V ++  
Sbjct: 648  DSLVARVLQARYFHSSNFLNAKIGSNPSYIWRSILWGRQIICGGSRWRIGNGQDVHIHKA 707

Query: 361  NWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI-LVRAGA 420
            NW+P    F+     T+  E  V++L+     W+E LI ++F   +  +I  I L R   
Sbjct: 708  NWIPKPFTFKPVIKPTMPSEALVSELINGENCWDEELIYKHFDKMDADVITQIPLPRRLR 767

Query: 421  EDKIVWHYEKSGLFSVKSGYRLGQSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIKIFL 480
            ED+++WH+ KSG ++VKSGY+          PSSS  ES+   W  +W + +P KI+IF+
Sbjct: 768  EDELIWHFGKSGQYTVKSGYQRALKIRFPAMPSSS--ESSKNEWNIIWSLALPKKIRIFV 827

Query: 481  WRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGLIHN 540
            WR   + LP+ +NL  R       C LC+ G E+  H    CK  K V   S F   I  
Sbjct: 828  WRAAKNLLPSAENLWKRKIVQEPTCQLCKMGIENVFHALVDCKAAKKVWRLSLFYIDIQA 887

Query: 541  VQAGSMFDLLREVKDKGPRTSG-------WASRMGDELYL----------VVPAGDLGLW 600
                 +  LL  VK               WA       +L          VV   +  + 
Sbjct: 888  APGQDILSLLHGVKRMRSNADVDLFAVMLWAKWNARNQWLFKGKRENPQSVVAKAEAVME 947

Query: 601  CWVRSVLAREDVR-----------WSPPEAGWYKVNVDASFRRERWQAGLGVVVRDSSGR 660
             + R V    DV            W+PP+ G+ K+N DA+   E+  AGLG V+RD +G+
Sbjct: 948  AYKR-VQPSADVSHGKQQKVAQLGWNPPQEGFVKINTDAATNSEKNLAGLGAVIRDENGQ 1007

Query: 661  VMLSASLVQRHVRSPEMAEGWAAVKGTRLAVEMGLGPLMLETDSSRVASFFQDEAGDDFS 683
            V  +A  V +   S   AE  A   G ++A +  +  +++E+DS  V S   +  G   S
Sbjct: 1008 VTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKDVIMESDSQEVVSLVNNRQGSR-S 1067

BLAST of Lag0021190 vs. NCBI nr
Match: XP_023889222.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC112001275 [Quercus suber])

HSP 1 Score: 473.0 bits (1216), Expect = 4.5e-129
Identity = 271/757 (35.80%), Postives = 388/757 (51.25%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
            MSKAYDRVEW+FLEKIM KMGF  +WV L+  CI++V +S  +NG     + PSRG+RQG
Sbjct: 848  MSKAYDRVEWLFLEKIMRKMGFDDKWVALLMECITTVSYSIMINGEPTSVIRPSRGIRQG 907

Query: 61   DPLSP-------------------------------------------------ANESDA 120
            DPLSP                                                 A+  + 
Sbjct: 908  DPLSPYLFLLCTEGLHNLLHQAAATGHIRGVSICKKGPRLTHLFFADDSLIFCRASMLEC 967

Query: 121  TAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSF 180
              V+ +L CYE+ SGQ +N  K+ + FS +T   +  Q+   L VQ      +YLGLP+ 
Sbjct: 968  QKVQSLLSCYEKASGQLLNLHKTSLFFSKATHPDILNQIKASLGVQEVKHCEKYLGLPTL 1027

Query: 181  MPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLIL 240
            + +++  S  +IK+RVW ++QGWK +L S  GREVLLK+V+QAIP Y+M+CF+LP  L  
Sbjct: 1028 IGKNKKESFRYIKERVWAKLQGWKEQLLSQAGREVLLKAVIQAIPTYAMSCFKLPITLCN 1087

Query: 241  EISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQN 300
            EI   + +FWWG     R IHW  W SLCKPK  GGMGFRD++ FN+A+LAKQ WR++ N
Sbjct: 1088 EIESLIKKFWWGQRGEQRKIHWSKWSSLCKPKTLGGMGFRDLQKFNEAMLAKQVWRLLTN 1147

Query: 301  PSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYG 360
              SL  R  K ++FP G  L A  G   SF W+S+L GR +++ G+ W+VGNG S+ +Y 
Sbjct: 1148 EDSLFYRFFKAKFFPNGSILEAEEG-NGSFAWKSILKGRVIIQKGMLWRVGNGSSIQIYH 1207

Query: 361  VNWLPDDGNFRVRSSG-TLAPETRVADLMTASGQ-WNEGLIRQNFSPQEVGLILSILVRA 420
             NWLPD    ++ S         +V+ LM   GQ W++ +I  NF P E  +I +I +  
Sbjct: 1208 DNWLPDPSLKKIISKPFHFDNHEKVSSLMAIGGQCWSQEVIDPNFLPSEAAIIKAIPLCF 1267

Query: 421  G-AEDKIVWHYEKSGLFSVKSGYRLGQSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIK 480
            G   D   W     G++SVKSGY+L     + + P SS        WKG+W + +PN++K
Sbjct: 1268 GECIDVRTWPLNTDGIYSVKSGYQLLVEQEINEQPRSSDLSKTKRLWKGIWSLKVPNRVK 1327

Query: 481  IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGL 540
              LWR   D LP+  NL  R   +   C  C    E+SLH  W C  ++ +  +  FG L
Sbjct: 1328 SLLWRAGSDSLPSKSNLKKRKIPIDATCSSCGMDPETSLHAIWSCPSLEQI-WKVHFGWL 1387

Query: 541  IHNV-QAGSMFDLLREVKDKG--------PRTSGWAS----RMGDE---LYLVVPAGDLG 600
            ++      S  D+L+   +K           +  W      R+G++   L ++       
Sbjct: 1388 VNAASMTASFLDVLQLCSEKSNLVDLFVMTVSQIWTRRNKLRVGEDAAPLDMINQLASAS 1447

Query: 601  LWCWVRSVLAREDV-------RWSPPEAGWYKVNVDASFRRERWQAGLGVVVRDSSGRVM 660
            L  +++S L    V       +W PP + W K+N D +   +   AGLG ++R+  G VM
Sbjct: 1448 LQVFIQSSLNPTKVPSPPKVTKWLPPPSNWVKINFDGATFSDLSSAGLGAIIRNDLGLVM 1507

Query: 661  LSASLVQRHVRSPEMAEGWAAVKGTRLAVEMGLGPLMLETDSSRVASFFQDEAGDDFSDV 683
             + + +     S EM E  AA      A E+G   +++E DS  V        G  FS +
Sbjct: 1508 AAFTHLIPLPTSVEMVEVLAARSALCFAKELGFNNIIVEGDSEIVIKALSTN-GPSFSSI 1567

BLAST of Lag0021190 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 4.6e-44
Identity = 144/565 (25.49%), Postives = 235/565 (41.59%), Query Frame = 0

Query: 128 LPSFMPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPK 187
           +P    R    +   I +RV  ++ GW+ K  S  GR  L K+V+ ++P +SM+   LP+
Sbjct: 1   MPVLQKRINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQ 60

Query: 188 KLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWR 247
            ++  + +    F WG     +  H V W  +C PK  GG+G R  +  N+AL++K  WR
Sbjct: 61  SILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWR 120

Query: 248 IIQNPSSLLARVLKGRYFPFGDFLRANVGTRP----SFIWRSLLWG-RELLEMGLRWQVG 307
           ++Q  +SL   VL+ +Y   G+ +R +    P    S  WRS+  G R+++  G+ W  G
Sbjct: 121 LLQEKNSLWTLVLQKKYH-VGE-IRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPG 180

Query: 308 NGESVSVYGVNW------LPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLI---RQN 367
           +G+ +  +   W      L  D   R     T+  +    DL      W+   I     N
Sbjct: 181 DGQQIRFWTDRWVSGKPLLELDNGERPTDCDTVVAK----DLWIPGRGWDFAKIDPYTTN 240

Query: 368 FSPQEVGLILSILVRAGAEDKIVWHYEKSGLFSVKSGYRLGQSTWLAQFPSSSSNESAMG 427
            +  E+  ++  LV  GA D++ W + + G FSV+S Y +     + + P      +   
Sbjct: 241 NTRLELRAVVLDLV-TGARDRLSWKFSQDGQFSVRSAYEM---LTVDEVP----RPNMAS 300

Query: 428 WWKGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHC 487
           ++  +WK+ +P ++K FLW +    + T +    R     NVC +C+ G ES LHV   C
Sbjct: 301 FFNCLWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDC 360

Query: 488 K-----FVKAV--------LMESEFGGLIHNV-------------------------QAG 547
                 +V+ V          +S F  L  N+                         + G
Sbjct: 361 PAQLGIWVRVVPQRRQQGFFSKSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCG 420

Query: 548 SMFDLLREVKDKGPRTSGWASRMGDELYLVVPAGDLGLWCWVRSVLAREDVRWSPPEAGW 607
           ++F    + +D+      WA         V  A    +   +        + W  P  GW
Sbjct: 421 NIFGENTKCRDRVKFVKEWAVE-------VYRAHSGNVLVGITQPRVERMIGWVSPCVGW 480

Query: 608 YKVNVDASFRRERWQAGLGVVVRDSSGRVMLSASLVQRHVRSPEMAEGWAAVKGTRLAVE 641
            KVN D + R     A  G V+RD +G      SL      +P+ AE W    G   A E
Sbjct: 481 VKVNTDGASRGNPGLASAGGVLRDCTGAWCGGFSLNIGRCSAPQ-AELWGVYYGLYFAWE 540

BLAST of Lag0021190 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 3.2e-37
Identity = 72/146 (49.32%), Postives = 98/146 (67.12%), Query Frame = 0

Query: 174 AIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPK-CYGGMGFRD 233
           A+P Y+M+CFRL K L  +++ AM  FWW   +  R I WV+W+ LCK K   GG+GFRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 234 MEIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGREL 293
           +  FNQALLAKQ +RII  P +LL+R+L+ RYFP    +  +VGTRPS+ WRS++ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 294 LEMGLRWQVGNGESVSVYGVNWLPDD 319
           L  GL   +G+G    V+   W+ D+
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDE 147

BLAST of Lag0021190 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 1.7e-158
Identity = 305/722 (42.24%), Postives = 418/722 (57.89%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLC-ISSVRFSFNVNGIRCGG--------- 60
            MSKAYDRVEW FLE +MLKMGF   W    + C +  +         R G          
Sbjct: 426  MSKAYDRVEWNFLEAVMLKMGFDFRWKVYQTYCNVLQIERVLRDYVSRVGALLLLTFSLL 485

Query: 61   VVPSRGLR-QGDPLSPANESDATAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQ 120
            ++ S  LR  G  +S A  +           Y + +GQ      ++I   P    R  + 
Sbjct: 486  MIVSYFLRLVGGKVSVAEIA-----------YRKCTGQ------ALIKL-PEYQDRQSSL 545

Query: 121  VGQILQVQVTAWHRQYLGLPSFMPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLK 180
            +  IL V +     QYLGLP+FMPR+R    ++IKDRVW+ +QGWK KLFS+GG+EVL+K
Sbjct: 546  IQNILSVNMVECQLQYLGLPTFMPRNRRMHFNYIKDRVWKHLQGWKAKLFSIGGKEVLIK 605

Query: 181  SVVQAIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMG 240
            +V QAIPCY+M+CFRLPK+LI E     ARFWWG  K D+ IHWV+W SL  PKC GGMG
Sbjct: 606  AVAQAIPCYTMSCFRLPKRLIREFHHITARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMG 665

Query: 241  FRDMEIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWG 300
            FRD+E+FN+ALLAKQCWRI+ +P+S+L+RVLKGRYF    F+ A +   PS+IWRS+LWG
Sbjct: 666  FRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWG 725

Query: 301  RELLEMGLRWQVGNGESVSVYGVNWLPDDGNFRVRSSGTLAPETRVADLMT-ASGQWNEG 360
            R+LL+ GLRW++GNG+SV +YG NW+P+    ++ SS  L   +RV+ L+    G W   
Sbjct: 726  RDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGD 785

Query: 361  LIRQNFSPQEVGLILSILVRAGA-EDKIVWHYEKSGLFSVKSGYRLG-QSTWLAQFPSSS 420
            ++R  F+P E   ILSI +  GA ED+++W+YEK+G++SV+SGY++   +    Q PSSS
Sbjct: 786  VVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSS 845

Query: 421  SNESAMGWWKGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESS 480
            S+E    WW G WKM IPNKIK+FLWRLCLDRLPT  NL  RG ++ N C  C R GE S
Sbjct: 846  SSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDS 905

Query: 481  LHVFWHCKFVKAVLMESEFGGLIHNVQAGSMFDLLREVKDKGPRTSG----------WAS 540
            +H+FW CKF +A+ + S+FG L       S F +LRE  +   +             W  
Sbjct: 906  IHLFWICKFAEALWINSKFGKL-------SPFLILRESHESLSKADFEELCVVIWGLWNQ 965

Query: 541  R----MGDELYLVVPAG-DLGLWCWVRSVLARE--------------DVRWSPPEAGWYK 600
            R      D    V   G +L  W    ++  RE              ++ W PP+ G YK
Sbjct: 966  RNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAEILWQPPDEGIYK 1025

Query: 601  VNVDASFRRERWQAGLGVVVRDSSGRVMLSASLVQRHVRSPEMAEGWAAVKGTRLAVEMG 660
            +N DASF      AGLG+++ +  G+VM +A+    +++S +MAE  AAV+G +LA E+G
Sbjct: 1026 INTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIG 1085

Query: 661  LGPLMLETDSSRVASFFQDEAGDDFSDVGALVAELRRDMPDPSFFGCRFTRREGNEVAHQ 680
            + P +                 +D S+ G +V + +            F +REGN+ AH 
Sbjct: 1086 MHPAL-----------------EDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHM 1105

BLAST of Lag0021190 vs. ExPASy TrEMBL
Match: A0A5E4FZN9 (PREDICTED: retrotransposon OS=Prunus dulcis OX=3755 GN=ALMOND_2B007697 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 2.7e-140
Identity = 293/766 (38.25%), Postives = 392/766 (51.17%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
            M+KAYDRVEWVFL ++MLK+GF+  WV  +  CIS+  FS    G   G ++P RGLRQG
Sbjct: 509  MAKAYDRVEWVFLREMMLKLGFSATWVAKVMDCISTTTFSMLWKGNPVGHIMPQRGLRQG 568

Query: 61   DPLSP-------------------------------------------------ANESDA 120
             PLSP                                                 A     
Sbjct: 569  CPLSPYLFLMCTEGFSCLLRGAERRGDLVGVQVARGGPSVTHLLFADDSILFMKATNEAC 628

Query: 121  TAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSF 180
             A+  +   YE VSGQ +N+ KS  S SP+        +  +L V V   H +YLGLP+ 
Sbjct: 629  RALETLFQTYEEVSGQQINYSKSAFSLSPNATRADFDMIKGVLNVPVVQCHEKYLGLPTI 688

Query: 181  MPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLIL 240
              + R      +KD++W+ I GWK KL S  G+E+L+K+V+QAIP YSM+CFR+PK L  
Sbjct: 689  AGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILMKAVLQAIPTYSMSCFRIPKGLCK 748

Query: 241  EISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQN 300
            E++  MARFWW   K  RGIHWV W+ LCK K  GG+GFRD+E FNQALLAKQCWRI++ 
Sbjct: 749  ELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRT 808

Query: 301  PSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYG 360
            P SL+AR+ + RY P   FL A VGT PSFIWRSL WG+ELL  GLRW+VGNG S+ VY 
Sbjct: 809  PESLVARIFRARYHPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGNGVSIQVYT 868

Query: 361  VNWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI-LVRAG 420
              WLP    F++ S   L   T V DL T+SGQWN  L++  F  QEV   L I L    
Sbjct: 869  DKWLPAPSFFKIMSPPQLPLSTLVCDLFTSSGQWNVPLLKDIFWDQEVDAKLQIPLASLA 928

Query: 421  AEDKIVWHYEKSGLFSVKSGYRLG--QSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIK 480
              D ++WHYE++G++SVKSGYRL   +   ++  PS   + ++  +WK +W + IPNKIK
Sbjct: 929  GHDCLIWHYERNGMYSVKSGYRLACLEKDKMSGEPSVRVDLNSK-FWKKIWALKIPNKIK 988

Query: 481  IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGL 540
             FLWR   D LP    L  R      +C  C R  ES LH  W C+  K V   S +G +
Sbjct: 989  FFLWRCAWDFLPCGQILFNRKIAPTPICPNCHRKAESVLHAVWLCETAKEVWRNSAWGNV 1048

Query: 541  IHNVQAGSMFDLLREV----------------------------KDKGPRTSGWASRMGD 600
                +  S  +L   +                            + K    +    RM  
Sbjct: 1049 CEEWRVNSFRELWHALQLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETATQLLHRMTK 1108

Query: 601  ELYLVVPAGDLGLWCWVRSVLAREDVR-WSPPEAGWYKVNVDASFRRERWQAGLGVVVRD 660
                   A +L      R    +  +  W PP AG YK+NVD + +      G+GVVVR+
Sbjct: 1109 LAQEFSNANNLSHTIHGRQSSPQAPLHGWRPPPAGIYKINVDGAVKSGDSVRGVGVVVRN 1168

Query: 661  SSGRVMLSASLVQRHVRS--PEMAEGWAAVKGTRLAVEMGLGPLMLETDSSR-VASFFQD 683
            ++G  M  A+ V+R   S      E  A ++G R A++MG    +LE D+   + S    
Sbjct: 1169 ANGEFM--AACVRRIQASYGARQTELMATIEGLRFAIDMGFTAAVLEMDAQDCINSILST 1228

BLAST of Lag0021190 vs. ExPASy TrEMBL
Match: M5W5F3 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa026368mg PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 1.8e-136
Identity = 295/769 (38.36%), Postives = 391/769 (50.85%), Query Frame = 0

Query: 1    MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
            M+KAYDRVEWVFL  +MLK+GF+  WV  +  CIS+  FS    G   G ++P RGLRQG
Sbjct: 280  MAKAYDRVEWVFLRAMMLKLGFSATWVSKVMDCISTTTFSVLWKGTPVGHIMPQRGLRQG 339

Query: 61   DPLSP-------------------------------------------------ANESDA 120
             PLSP                                                 A   D 
Sbjct: 340  CPLSPYLFLICTEGFSCLLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKDC 399

Query: 121  TAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSF 180
             A+  +   YE V+GQ +N+ KS +S SP+        +  +L V V   H  YLGLP+ 
Sbjct: 400  MALETLFQTYEEVTGQQINYSKSALSLSPNATRADFDMIEGVLNVPVVRCHENYLGLPTI 459

Query: 181  MPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLIL 240
              + R      +KD++W+ I GWK KL S  G+E+L+K+V+QAIP YSM+CFR+PK L  
Sbjct: 460  AGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFRIPKGLCK 519

Query: 241  EISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQN 300
            E++  MARFWW   K  RGIHWV W+ LCK K  GG+GFRD+E FNQALLAKQCWRI++ 
Sbjct: 520  ELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRT 579

Query: 301  PSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYG 360
            P SL+AR+ + RY P   FL A VGT PSFIWRSL WG+ELL  GLRW+VG+G S+ VY 
Sbjct: 580  PESLVARIFRARYHPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGSGVSIQVYT 639

Query: 361  VNWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI-LVRAG 420
              WLP    F++ S   L   TRV DL T+SGQWN  L++  F  QEV  IL I L    
Sbjct: 640  DKWLPAPSCFKIMSPPQLPLSTRVCDLFTSSGQWNVPLLKDIFWDQEVDAILQIPLASLA 699

Query: 421  AEDKIVWHYEKSGLFSVKSGYRLG--QSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIK 480
              D ++WHYE++G++SVKSGYRL   +   ++  PS+  + ++  +WK +W + IPNKIK
Sbjct: 700  GHDCLIWHYERNGMYSVKSGYRLAGLEKDKMSGEPSARVDLNSK-FWKKIWALKIPNKIK 759

Query: 481  IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGL 540
             FLWR   D LP    L  R      +C  C R  ES LH  W C+  K V   S +G +
Sbjct: 760  FFLWRCAWDFLPCGQILFNRKIAPTPICPKCHRKAESVLHAVWLCEAAKEVWRNSAWGNV 819

Query: 541  IHNVQAGSMFDLLREVKDKGPRTSGWASRMGDELYLVVPAGDLGLW-------------- 600
                +  S  +L   +     + S      G   YL       GLW              
Sbjct: 820  CEVWRVNSFRELWHAL-----QLSSSGEEQGLFAYLC-----WGLWNRRNSFIFEGKSET 879

Query: 601  ---------------CWVRSVLAREDVRWSPPEA---GWYKVNVDASFRRERWQAGLGVV 660
                               ++L     R S P+A   GW       S    R   G+GVV
Sbjct: 880  AIQLLSRMTKLAQEFSDANNILHTIHGRQSSPQAPLQGWRPPPAVKSGDSVR---GVGVV 939

Query: 661  VRDSSGRVMLSASLVQRHVRS--PEMAEGWAAVKGTRLAVEMGLGPLMLETDSSR-VASF 683
            VR+++G  M  A+ V+R   S      E  A ++G R A++MG    +LE D+   + S 
Sbjct: 940  VRNANGEFM--AACVRRIHASYGARQTELMATIEGLRFAIDMGFTDAILEMDAQDCLNSI 999

BLAST of Lag0021190 vs. ExPASy TrEMBL
Match: A0A251NPF0 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G123900 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 1.8e-136
Identity = 295/769 (38.36%), Postives = 391/769 (50.85%), Query Frame = 0

Query: 1   MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
           M+KAYDRVEWVFL  +MLK+GF+  WV  +  CIS+  FS    G   G ++P RGLRQG
Sbjct: 243 MAKAYDRVEWVFLRAMMLKLGFSATWVSKVMDCISTTTFSVLWKGTPVGHIMPQRGLRQG 302

Query: 61  DPLSP-------------------------------------------------ANESDA 120
            PLSP                                                 A   D 
Sbjct: 303 CPLSPYLFLICTEGFSCLLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKDC 362

Query: 121 TAVRGILDCYERVSGQTVNFDKSIISFSPSTDGRVKAQVGQILQVQVTAWHRQYLGLPSF 180
            A+  +   YE V+GQ +N+ KS +S SP+        +  +L V V   H  YLGLP+ 
Sbjct: 363 MALETLFQTYEEVTGQQINYSKSALSLSPNATRADFDMIEGVLNVPVVRCHENYLGLPTI 422

Query: 181 MPRDRTSSLSFIKDRVWQQIQGWKGKLFSVGGREVLLKSVVQAIPCYSMNCFRLPKKLIL 240
             + R      +KD++W+ I GWK KL S  G+E+L+K+V+QAIP YSM+CFR+PK L  
Sbjct: 423 AGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFRIPKGLCK 482

Query: 241 EISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDMEIFNQALLAKQCWRIIQN 300
           E++  MARFWW   K  RGIHWV W+ LCK K  GG+GFRD+E FNQALLAKQCWRI++ 
Sbjct: 483 ELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRT 542

Query: 301 PSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYG 360
           P SL+AR+ + RY P   FL A VGT PSFIWRSL WG+ELL  GLRW+VG+G S+ VY 
Sbjct: 543 PESLVARIFRARYHPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGSGVSIQVYT 602

Query: 361 VNWLPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI-LVRAG 420
             WLP    F++ S   L   TRV DL T+SGQWN  L++  F  QEV  IL I L    
Sbjct: 603 DKWLPAPSCFKIMSPPQLPLSTRVCDLFTSSGQWNVPLLKDIFWDQEVDAILQIPLASLA 662

Query: 421 AEDKIVWHYEKSGLFSVKSGYRLG--QSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIK 480
             D ++WHYE++G++SVKSGYRL   +   ++  PS+  + ++  +WK +W + IPNKIK
Sbjct: 663 GHDCLIWHYERNGMYSVKSGYRLAGLEKDKMSGEPSARVDLNSK-FWKKIWALKIPNKIK 722

Query: 481 IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAVLMESEFGGL 540
            FLWR   D LP    L  R      +C  C R  ES LH  W C+  K V   S +G +
Sbjct: 723 FFLWRCAWDFLPCGQILFNRKIAPTPICPKCHRKAESVLHAVWLCEAAKEVWRNSAWGNV 782

Query: 541 IHNVQAGSMFDLLREVKDKGPRTSGWASRMGDELYLVVPAGDLGLW-------------- 600
               +  S  +L   +     + S      G   YL       GLW              
Sbjct: 783 CEVWRVNSFRELWHAL-----QLSSSGEEQGLFAYLC-----WGLWNRRNSFIFEGKSET 842

Query: 601 ---------------CWVRSVLAREDVRWSPPEA---GWYKVNVDASFRRERWQAGLGVV 660
                              ++L     R S P+A   GW       S    R   G+GVV
Sbjct: 843 AIQLLSRMTKLAQEFSDANNILHTIHGRQSSPQAPLQGWRPPPAVKSGDSVR---GVGVV 902

Query: 661 VRDSSGRVMLSASLVQRHVRS--PEMAEGWAAVKGTRLAVEMGLGPLMLETDSSR-VASF 683
           VR+++G  M  A+ V+R   S      E  A ++G R A++MG    +LE D+   + S 
Sbjct: 903 VRNANGEFM--AACVRRIHASYGARQTELMATIEGLRFAIDMGFTDAILEMDAQDCLNSI 962

BLAST of Lag0021190 vs. ExPASy TrEMBL
Match: M5XK32 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016563mg PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 1.5e-133
Identity = 275/706 (38.95%), Postives = 400/706 (56.66%), Query Frame = 0

Query: 1   MSKAYDRVEWVFLEKIMLKMGFAPEWVDLISLCISSVRFSFNVNGIRCGGVVPSRGLRQG 60
           MSKAYDRVEW FLEK+ML MGF   WV ++  C+++V +SF VNG     + P+RGLRQG
Sbjct: 176 MSKAYDRVEWEFLEKMMLAMGFPILWVRMVMDCVTTVSYSFLVNGEPTRILYPTRGLRQG 235

Query: 61  DPLSP-------------------ANESDATAVRGILDCYERVSGQTVNFDKSIISFSPS 120
           DPLSP                   A +++   ++ I + YER SG+ +N  KS ++FS +
Sbjct: 236 DPLSPYLFLLCAEVLPHDSFVFAKATDNNCGVLKHIFEVYERASGEQINCQKSCVAFSAN 295

Query: 121 TDGRVKAQVGQILQVQVTAWHRQYLGLPSFMPRDRTSSLSFIKDRVWQQIQGWKGKLFSV 180
                ++++  +L V     H  YLGLP  + R++T    ++K+RVW+++QGW+ +  S+
Sbjct: 296 IHMDTQSRLASVLGVPRVDSHATYLGLPMMLGRNKTFCFRYLKERVWKKLQGWREQTLSI 355

Query: 181 GGREVLLKSVVQAIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCK 240
            G+EVLLK V Q+IP Y MNCF LP+ L  EI + MARFWWG +  +R IHW+ W+ LCK
Sbjct: 356 AGKEVLLKVVAQSIPLYVMNCFLLPQGLCHEIEQMMARFWWGQQGENRKIHWMRWERLCK 415

Query: 241 PKCYGGMGFRDMEIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSF 300
            K  GGMGFR ++ FN A+LAKQ WR++ NP SL +R+LK +YFP  +F  A +G+RPS 
Sbjct: 416 AKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPHSLASRLLKAKYFPHTNFWEATLGSRPSC 475

Query: 301 IWRSLLWGRELLEMGLRWQVGNGESVSVYGVNWLPDDGNFRVRSSGTLAPE-TRVADLMT 360
           +W+S+   R++LEMG R+Q+G+G+SV ++G  W+P    F V +S     E T+V++L+ 
Sbjct: 476 VWKSIWTARKVLEMGSRFQIGDGKSVRIWGDKWVPRPAAFVVITSPLDGMENTKVSELIC 535

Query: 361 ASG--QWNEGLIRQNFSPQEVGLILSILVRAGA-EDKIVWHYEKSGLFSVKSGYRLG-QS 420
             G  QW+   +   F P +V   + I +   A  D+IVW+Y+K GLF+VKS YR+  + 
Sbjct: 536 NEGSPQWDLQKLNNLFLPVDVVDFVRIPLSIRAPPDRIVWNYDKHGLFTVKSAYRVALRV 595

Query: 421 TWLAQFPSSSSNESAMGWWKGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVC 480
           T   +  SSSSN      W+ +W   +P K+KIF WR+  D LPT  NL  +G D+ ++C
Sbjct: 596 TSGDEDESSSSNSDTSMLWRHIWNATVPTKLKIFAWRVAHDILPTKANLIKKGVDMQDMC 655

Query: 481 GLCRRGGESSLHVFWHCKFVKAVLMESEFGGLIHNVQAGSMFDLLREVKDKGPRTSGWAS 540
             C    ES+LHV   C F  A    S      H     S  D++           G+A 
Sbjct: 656 MFCGDITESALHVLAMCPFAVATWNISLLTRHAHQGVQRSPHDVV-----------GFAQ 715

Query: 541 RMGDELYLVVPAGDLGLWCWVRSVLAREDVRWSPPEAGWYKVNVDASFRRERWQAGLGVV 600
           +   E    + A D       R    R+ VRW+ P +G  K N D +F     +  +GVV
Sbjct: 716 QYVHEF---ITANDTPSKVTDR---VRDPVRWAAPSSGRLKFNFDGAFDPTSGRGAVGVV 775

Query: 601 VRDSSGRVMLSASLVQRHVRSPEMAEGWAAVKGTRLAVEMGLGPLMLETDSSRVASFFQD 660
            RD+ G  + + +     V S E AE  AA +G  LA+ +G    + E DS+ V S  + 
Sbjct: 776 ARDADGGFVAAVAKSVGEVLSAEHAEILAAREGVALALSLGTASPIFEGDSAVVVSAVK- 835

Query: 661 EAGDDFSDVGALVAE---LRRDMPDPSFFGCRFTRREGNEVAHQLA 680
            AG D+S++G +V +   L++  P   F   +FT RE N V H+LA
Sbjct: 836 RAGQDYSNIGTIVEDVKHLQQQFPSSLF---QFTPREANGVVHRLA 860

BLAST of Lag0021190 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 1.4e-59
Identity = 163/556 (29.32%), Postives = 252/556 (45.32%), Query Frame = 0

Query: 174 AIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPKCYGGMGFRDM 233
           A+P Y+M CF LPK +  +I   +A FWW  ++  +G+HW +W  L   K  GG+GF+D+
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 234 EIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELL 293
           E FN ALL KQ WR++  P SL+A+V K RYF   D L A +G+RPSF+W+S+   +E+L
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 294 EMGLRWQVGNGESVSVYGVNWL---PDDGNFRV-----RSSGTLAPETRVADLMTASG-Q 353
             G R  VGNGE + ++   WL   P     R+     +   +++   +V+DL+  SG +
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGRE 181

Query: 354 WNEGLIRQNFSPQEVGLILSILVRAGAE---DKIVWHYEKSGLFSVKSGY-RLGQSTWLA 413
           W + +I   F   E  LI  +  R G     D   W Y  SG ++VKSGY  L Q     
Sbjct: 182 WRKDVIEMLFPEVERKLIGEL--RPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKR 241

Query: 414 QFPSSSSNESAMGWWKGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCR 473
             P   S  S    ++ +WK     KI+ FLW+   + LP    L  R     + C  C 
Sbjct: 242 SSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCP 301

Query: 474 RGGESSLHVFWHCKFVKAVLMESEFGGLIHNVQAGSMFDLLREVKDKGPRTSGW--ASRM 533
              E+  H+ + C F +     S     +    A S++  L  V + G     W  AS++
Sbjct: 302 SCKETVNHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKASQL 361

Query: 534 ------------------GDEL----YLVVPAGDLGLW--------CWVRSVLAREDV-R 593
                             G E      L     DL  W        C  +  + R    R
Sbjct: 362 VPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGR 421

Query: 594 WSPPEAGWYKVNVDASFRRERWQAGLGVVVRDSSGRVMLSASLVQRHVRSPEMAE----G 653
           W PP   W K N DA++ R+  + G+G V+R+  G V    +     ++S   AE     
Sbjct: 422 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 481

Query: 654 WAAVKGTRLAVEMGLGPLMLETDSSRVASFFQDEAGDDFSDVGALVAELRRDMPDPSFFG 680
           WA +  +R         ++ E+DS  +     ++  + +  +   + +L+R +   +   
Sbjct: 482 WAVLSLSRFQYNY----VIFESDSQVLIEILNND--EIWPSLKPTIQDLQRLLSQFTEVK 541

BLAST of Lag0021190 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 158.3 bits (399), Expect = 2.3e-38
Identity = 72/146 (49.32%), Postives = 98/146 (67.12%), Query Frame = 0

Query: 174 AIPCYSMNCFRLPKKLILEISRAMARFWWGGEKVDRGIHWVSWKSLCKPK-CYGGMGFRD 233
           A+P Y+M+CFRL K L  +++ AM  FWW   +  R I WV+W+ LCK K   GG+GFRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 234 MEIFNQALLAKQCWRIIQNPSSLLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGREL 293
           +  FNQALLAKQ +RII  P +LL+R+L+ RYFP    +  +VGTRPS+ WRS++ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 294 LEMGLRWQVGNGESVSVYGVNWLPDD 319
           L  GL   +G+G    V+   W+ D+
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDE 147

BLAST of Lag0021190 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-29
Identity = 112/465 (24.09%), Postives = 193/465 (41.51%), Query Frame = 0

Query: 260 LKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYGVNWLPDDG 319
           +K RYF     L A V  + S+ W SLL G  LL+ G R  +G+G+++ + G++ + D  
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRI-GLDNIVDSH 60

Query: 320 NFRVRSSGTLAPETRVADLMTASGQ---WNEGLIRQNFSPQEVGLILSI-LVRAGAEDKI 379
             R  ++     E  + +L    G    W++  I Q     + G I  I L ++   DKI
Sbjct: 61  PPRPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKI 120

Query: 380 VWHYEKSGLFSVKSGYRLGQSTWLAQFPSSSSNESAMGWWKGVWKMFIPNKIKIFLWRLC 439
           +W+Y  +G ++V+SGY L         P+ +    ++     +W + I  K+K FLWR  
Sbjct: 121 IWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRAL 180

Query: 440 LDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKF------------VKAVLMES 499
              L T + L  RG  +   C  C R  ES  H  + C F            ++  LM +
Sbjct: 181 SQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSN 240

Query: 500 EF----GGLIHNVQAGSMFDLLREV------KDKGPRTSGWASRMGDELYLVVPAGDLGL 559
           +F      +++ VQ  +M D  + +      +    R +   ++  +     V +     
Sbjct: 241 DFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAET 300

Query: 560 WCWVRSV------------LAREDVRWSPPEAGWYKVNVDASFRRERWQAGLGVVVRDSS 619
             W+ +             +A   + W  P A + K N DA F  ++ +A  G ++R+  
Sbjct: 301 HDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHY 360

Query: 620 GRVMLSASLVQRHVRSPEMAEGWAAVKGTRLAVEMGLGPLMLETDSSRVASFFQDEAGDD 679
           G  +   S+   H  +P  AE  A +   +     G   + +E D   + +      G  
Sbjct: 361 GTPISWGSMKLAHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLIN---GIS 420

BLAST of Lag0021190 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 1.6e-12
Identity = 67/306 (21.90%), Postives = 114/306 (37.25%), Query Frame = 0

Query: 416 KGVWKMFIPNKIKIFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKF 475
           + +WK+ +  KIK FLWR     L T   L  R  D   +C  C    E+  H+ ++C +
Sbjct: 35  QAIWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPY 94

Query: 476 VKAVLMESEFGGLIHNVQAG--SMFD--LLREVKDKGPRTSG--------------WASR 535
            ++V   +    +I   Q G  S F+  L R ++    +T+               W SR
Sbjct: 95  TQSVWRSA---NIIIGNQWGPPSSFEDNLNRLIQLSKTQTTNSLDRFLPFWIMWRLWKSR 154

Query: 536 ---------MGDELYLVVPAGDLGLWCWVRSVL---------------AREDVRWSPPEA 595
                       +        D   W                       R+  +W+PP  
Sbjct: 155 NVFLFQQKCQSPDYEARKGIQDATEWLNANETTENTNVHVATNPIQTSRRDSSQWNPPPE 214

Query: 596 GWYKVNVDASFRRERWQAGLGVVVRDSSGRVMLSASLVQRHVRSPEMAEGWAAVKGTRLA 655
           GW K N D+ + +       G  +R+ +G ++L  +   +       AE    +   ++ 
Sbjct: 215 GWVKCNFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSLHAEALGFLHALQVI 274

Query: 656 VEMGLGPLMLETDSSRVASFFQDEAGDDFSDVGALVAELRRDMPDPSFFGCRFTRREGNE 680
              GL  +  E+DS  + +   +  G+D S +G L+ ++R  M    +    F  RE N 
Sbjct: 275 WAHGLRYVWFESDSKSLVTLINN--GEDHSLLGTLIYDIRHWMLKLPYCSLEFVNRERNS 334

BLAST of Lag0021190 vs. TAIR 10
Match: AT1G43730.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 63.5 bits (153), Expect = 7.7e-10
Identity = 65/231 (28.14%), Postives = 89/231 (38.53%), Query Frame = 0

Query: 255 LLARVLKGRYFPFGDFLRANVGTRPSFIWRSLLWGRELLEMGLRWQVGNGESVSVYGVNW 314
           LLA  L   +    +F   N  T  S+IWR L   RE+    +   VG+G +   +  NW
Sbjct: 25  LLASELVENFLGQRNFWTLNSTTSDSWIWRRLCKLREVARPFVVCDVGSGVTAKFWHDNW 84

Query: 315 LPDDGNFRVRSSGTLAPETRVADLMTASGQWNEGLIRQNFSPQEVGLILSI--LVRAGAE 374
                       G L       DL+   G            PQ VGL +    L+    +
Sbjct: 85  ---------TGHGPL------IDLVGPLG------------PQTVGLPIDAVGLIDCQHD 144

Query: 375 DKIVWH---YEKSGLFSVKSGYRLGQSTWLAQFPSSSSNESAMGWWKGVW-KMFIPNKIK 434
           D  +W    +  S +FS          T LA  P +      + W+K VW K  +P K  
Sbjct: 145 DSFIWKTDLHAPSNIFST-------AKTSLALHPQN----HIVPWYKAVWFKNHVP-KHA 204

Query: 435 IFLWRLCLDRLPTIDNLGVRGCDVLNVCGLCRRGGESSLHVFWHCKFVKAV 480
              W +  +RL T D L   G  +  VC LC    ES  H+F+ C F  AV
Sbjct: 205 FICWVVAWNRLHTRDRLRSWGLSIPAVCLLCNSHDESRAHLFFECPFCGAV 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150918.13.5e-15842.24uncharacterized protein LOC111018954 [Momordica charantia][more]
VVA32947.15.7e-14038.25PREDICTED: retrotransposon [Prunus dulcis][more]
ONI01138.13.8e-13638.36hypothetical protein PRUPE_6G123900 [Prunus persica][more]
XP_024956542.19.1e-13035.05uncharacterized protein LOC112498908 [Citrus sinensis][more]
XP_023889222.14.5e-12935.80LOW QUALITY PROTEIN: uncharacterized protein LOC112001275 [Quercus suber][more]
Match NameE-valueIdentityDescription
P0C2F64.6e-4425.49Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P932953.2e-3749.32Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DAR41.7e-15842.24uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A5E4FZN92.7e-14038.25PREDICTED: retrotransposon OS=Prunus dulcis OX=3755 GN=ALMOND_2B007697 PE=4 SV=1[more]
M5W5F31.8e-13638.36Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
A0A251NPF01.8e-13638.36Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRU... [more]
M5XK321.5e-13338.95Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
Match NameE-valueIdentityDescription
AT4G29090.11.4e-5929.32Ribonuclease H-like superfamily protein [more]
ATMG00310.12.3e-3849.32RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G09510.11.1e-2924.09Ribonuclease H-like superfamily protein [more]
AT2G02650.11.6e-1221.90Ribonuclease H-like superfamily protein [more]
AT1G43730.17.7e-1028.14RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 406..478
e-value: 5.8E-17
score: 62.2
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 2..66
e-value: 2.6E-8
score: 33.6
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 559..679
e-value: 2.1E-18
score: 66.3
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 555..683
e-value: 7.4E-11
score: 44.2
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..431
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 1..431
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 558..679
e-value: 1.12741E-16
score: 74.658
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 554..682

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021190.1Lag0021190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity