Lag0021105 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0021105
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Actin-depolymerizing factor
Location	chr7: 4697649 .. 4699361 (+)
RNA-Seq Expression	Lag0021105
Synteny	Lag0021105

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGAAAAGAGGTGGTGATGGAAAAGACTGGGAGCTCAGCCGAGAGTTTCAGTGATTTCACTGCTTCATTACATGAGAATGATTGTAGATATGCAGTTTATGACTACAATTTTGTGACTTCTGAGAACTGCCAAAAGAGCAAAGTCTTTTCCATTGCCTGGTTAGTTATTATAATTAATTTAAACAGTTTCTTTTCCTGGTTAGTTCAGTAATTTGACTTACAGTTTAGTTTTCTTCCTGTTCCTTTTATGCTAGATTTCAATTTCAACCTCTTTCTGTTTGTGTTAAACGAGTATTGCATTTATTTATTGGAGTTCCATTACTATATCTGCGAGATTCTGCTTTAATTTCATGTTTCTTAACTATTTCTTTATATCAAGCATCTTTCTTTATGTGTTGAATGTACACATCAGGTGCCATTTCTAACATAGCTATCATGAATTAAAAACATAAATGGCTTATGATATGAATGAAGGGTGGAAATTGTGGGGAGCTATCACATTCCTAATGTATTTGGTTGACTTTCTTTCTGATATGGTTTCATAAAATTGTTTCTTAACTATTTCATTATATTGAGCATCTTTCTTTCTGATTGAGAATTTTGGAAATTTATACAGAGGGTGGTTTTAACTTTTAATATCAGTTTGTTGTCAATTTGCTCCAATTAATTGGCCCGGTTGATTGTAATAGCACTACCATATTTTTGAGTTTCCGTCCTCTGCTGTGCTGTAGTGCCAAACATCCATATCATGATCATTGATTCATAGCCATCTTTATTGCAATATTTTTGCAATGCAGTCTGGTTCTACAAGGCATTGGTAATTCCTGCCAGTCTTTCCCACTCTATCCTCTTGGGGACCCTTCCAGTCTGTAGTTTTCACACCTGATTTCTTCTATTTTCTGGCCACTGGTAATCACATTCAATGTTCAATATGGTCATTCTAGTTCTGCAGGCACATGAAATGTAGTGGTATGCAACTGTTTGATATAGTCTTCCTCGAGATGATGATAGGCATTGTCCAACAATTTTATTTATAATCACATGGCAGGGTAACAAGAGAAGATTAGAGAGCTTCCTGCCTCTCCCCCAATTCTCATCAGAGAATATTACAAAATAGTTTAGCAAAAGGATACCCTTCCCAAGCAAACAAACAACTCTATATTCCCTACTCTCCAGTCTCCACGGCCCTGTCCAACTAACAGAATACTCTGGCCCACCACACACAAACATTCTTTTCTCACACACCCACACCCCTCCTTACTTGCTTATGTGTTTATACTTCTGTAATTGGTGGCTCTCGGATGATTAATTGAGCCTAAAGCTCTCTCAAAGAACTCAAAGGAAATCTTTATTTAACGTTTGCAAAATATGAAGAATAAGTACACCAACTGAAAGCATTTAACTAAAATGAAATTACAAACTTATCGCAAAATATAGAGAATCTTTATTTTAATGTTCACAAAATATAGAGAATAAGTACACTAACTAAAATGAAATTACAAAATTATCCTACTATTCCCTACATCAATGATTTACTTTAGTTCTACGTCTTACGAGCAACTCTTTTTACAGGTCGATTCGTTCCAAGATGCTTTATGCAACATCAAAGGACAGGTTTAGAAGGGAATGGGAGGGAATCCATTACGAGATACAGGCAACTGACCCAACTGAGATGGATCTTGAAGTCCTTAGAGACCGTGCAAACTGA

mRNA sequence

ATGAAAAGAAAAGAGGTGGTGATGGAAAAGACTGGGAGCTCAGCCGAGAGTTTCAGTGATTTCACTGCTTCATTACATGAGAATGATTGTAGATATGCAGTTTATGACTACAATTTTGTGACTTCTGAGAACTGCCAAAAGAGCAAAGTCTTTTCCATTGCCTGGTCGATTCGTTCCAAGATGCTTTATGCAACATCAAAGGACAGGTTTAGAAGGGAATGGGAGGGAATCCATTACGAGATACAGGCAACTGACCCAACTGAGATGGATCTTGAAGTCCTTAGAGACCGTGCAAACTGA

Coding sequence (CDS)

ATGAAAAGAAAAGAGGTGGTGATGGAAAAGACTGGGAGCTCAGCCGAGAGTTTCAGTGATTTCACTGCTTCATTACATGAGAATGATTGTAGATATGCAGTTTATGACTACAATTTTGTGACTTCTGAGAACTGCCAAAAGAGCAAAGTCTTTTCCATTGCCTGGTCGATTCGTTCCAAGATGCTTTATGCAACATCAAAGGACAGGTTTAGAAGGGAATGGGAGGGAATCCATTACGAGATACAGGCAACTGACCCAACTGAGATGGATCTTGAAGTCCTTAGAGACCGTGCAAACTGA

Protein sequence

MKRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWSIRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN

Homology

[Close]

BLAST of Lag0021105 vs. NCBI nr
Match: KAA8527768.1 (hypothetical protein F0562_035363 [Nyssa sinensis])

HSP 1 Score: 169.5 bits (428), Expect = 1.5e-38
Identity = 85/103 (82.52%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+KEVV+EKTG  AES+ DFTASL ENDCRYAVYD++FVTSENCQKSK+F IAWS     
Sbjct: 41  KKKEVVIEKTGGPAESYDDFTASLPENDCRYAVYDFDFVTSENCQKSKIFFIAWSPSVSR 100

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSKDRFRRE EGIHYEIQATDPTEMDLEVLRDRAN
Sbjct: 101 IRAKMLYATSKDRFRRELEGIHYEIQATDPTEMDLEVLRDRAN 143

[Close]

BLAST of Lag0021105 vs. NCBI nr
Match: XP_023914794.1 (actin-depolymerizing factor [Quercus suber] >XP_030922753.1 actin-depolymerizing factor-like [Quercus lobata] >KAF3974937.1 hypothetical protein CMV_001781 [Castanea mollissima])

HSP 1 Score: 169.5 bits (428), Expect = 1.5e-38
Identity = 85/103 (82.52%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+KEVV+EKTG  AES+ DFTASL ENDCRYAVYD++FVTSENCQKSK+F IAWS     
Sbjct: 41  KKKEVVVEKTGGPAESYDDFTASLPENDCRYAVYDFDFVTSENCQKSKIFFIAWSPSVSR 100

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSKDRFRRE EGIHYEIQATDPTEMDLEVLRDRAN
Sbjct: 101 IRAKMLYATSKDRFRRELEGIHYEIQATDPTEMDLEVLRDRAN 143

[Close]

BLAST of Lag0021105 vs. NCBI nr
Match: XP_028780695.1 (actin-depolymerizing factor-like [Prosopis alba] >XP_028780703.1 actin-depolymerizing factor-like [Prosopis alba] >XP_028795660.1 actin-depolymerizing factor-like [Prosopis alba])

HSP 1 Score: 169.5 bits (428), Expect = 1.5e-38
Identity = 85/103 (82.52%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+KEVV+EKTG  AES+ DFTASL ENDCRYAVYD++FVTSENCQKSK+F IAWS     
Sbjct: 43  KKKEVVVEKTGGPAESYDDFTASLPENDCRYAVYDFDFVTSENCQKSKIFFIAWSPSVSR 102

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSKDRFRRE EGIHYEIQATDPTEMDLEVLRDRAN
Sbjct: 103 IRAKMLYATSKDRFRRELEGIHYEIQATDPTEMDLEVLRDRAN 145

[Close]

BLAST of Lag0021105 vs. NCBI nr
Match: XP_041001240.1 (actin-depolymerizing factor-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 168.7 bits (426), Expect = 2.5e-38
Identity = 85/103 (82.52%), Postives = 91/103 (88.35%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+KEVV+EKTG  AES+ DFTASL ENDCRYAVYDY+FVTSENCQKSK+F IAWS     
Sbjct: 43  KKKEVVVEKTGGPAESYDDFTASLPENDCRYAVYDYDFVTSENCQKSKIFFIAWSPAVSR 102

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSK RFRRE EGIHYEIQATDPTEMDLEVLRDRAN
Sbjct: 103 IRAKMLYATSKQRFRRELEGIHYEIQATDPTEMDLEVLRDRAN 145

[Close]

BLAST of Lag0021105 vs. NCBI nr
Match: THG07562.1 (hypothetical protein TEA_022966 [Camellia sinensis var. sinensis])

HSP 1 Score: 168.7 bits (426), Expect = 2.5e-38
Identity = 84/103 (81.55%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+KEVV+EKTG  AES+ DFTASL ENDCRYAVYDY+FVTSENCQKSK+F IAWS     
Sbjct: 51  KKKEVVVEKTGGPAESYDDFTASLPENDCRYAVYDYDFVTSENCQKSKIFFIAWSPAVSR 110

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSKDRFRRE EGIHYEIQATDPTEMDLEV++DRAN
Sbjct: 111 IRAKMLYATSKDRFRRELEGIHYEIQATDPTEMDLEVIQDRAN 153

[Close]

BLAST of Lag0021105 vs. ExPASy Swiss-Prot
Match: Q8SAG3 (Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 2.0e-38
Identity = 78/103 (75.73%), Postives = 91/103 (88.35%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+KEVV+EKTG  AESF +F A+L ENDCRYAVYD++FVTSENCQKSK+F IAWS     
Sbjct: 41  KKKEVVVEKTGGPAESFDEFAAALPENDCRYAVYDFDFVTSENCQKSKIFFIAWSPDSSR 100

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSK+RFRRE +G+HYEIQATDPTEMDLEVLR+RA+
Sbjct: 101 IRAKMLYATSKERFRRELDGVHYEIQATDPTEMDLEVLRERAH 143

[Close]

BLAST of Lag0021105 vs. ExPASy Swiss-Prot
Match: Q2QLT8 (Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 9.3e-36
Identity = 72/103 (69.90%), Postives = 89/103 (86.41%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K KEVV+EKTGSS ESF DF  SL E+DCRYA+YD++FVT ENCQKSK+F +AWS     
Sbjct: 43  KCKEVVVEKTGSSTESFDDFMDSLPESDCRYAIYDFDFVTEENCQKSKIFFVAWSPSVSR 102

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLYATSK+RFRRE +G+HYEIQATDP+E+D+E+LR+RA+
Sbjct: 103 IRAKMLYATSKERFRRELDGVHYEIQATDPSELDIELLRERAH 145

[Close]

BLAST of Lag0021105 vs. ExPASy Swiss-Prot
Match: Q9ZSK2 (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 4.6e-35
Identity = 72/102 (70.59%), Postives = 87/102 (85.29%), Query Frame = 0


Query: 3   RKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS-----I 62
           +KEVV+EKTG+  ES+ DF ASL +NDCRYAVYD++FVTSENCQKSK+F  AWS     I
Sbjct: 45  KKEVVVEKTGNPTESYDDFLASLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGI 104

Query: 63  RSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           R+K+LY+TSKD+  RE +GIHYEIQATDPTE+DLEVLR+RAN
Sbjct: 105 RAKVLYSTSKDQLSRELQGIHYEIQATDPTEVDLEVLRERAN 146

[Close]

BLAST of Lag0021105 vs. ExPASy Swiss-Prot
Match: Q9AY76 (Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 5.1e-34
Identity = 70/103 (67.96%), Postives = 88/103 (85.44%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAWS----- 61
           K+K+VV+EKTG++ ES+ DF ASL ENDCRYA+YD++FVT EN QKSK+F IAWS     
Sbjct: 43  KQKQVVVEKTGATTESYDDFLASLPENDCRYALYDFDFVTGENVQKSKIFFIAWSPSTSR 102

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           IR+KMLY+TSKDR ++E +G HYEIQATDPTE+DLEVLR+RA+
Sbjct: 103 IRAKMLYSTSKDRIKQELDGFHYEIQATDPTEVDLEVLRERAH 145

[Close]

BLAST of Lag0021105 vs. ExPASy Swiss-Prot
Match: Q9FVI2 (Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 5.3e-31
Identity = 64/103 (62.14%), Postives = 83/103 (80.58%), Query Frame = 0


Query: 2   KRKEVVMEKTGSSAESFSDFTASLHENDCRYAVYDYNFVTSENCQKSKVFSIAW-----S 61
           K+K+VV+EK G   ES+ DF ASL EN+CRYAVYD++FVT+ENCQKS++F IAW      
Sbjct: 37  KQKQVVVEKIGEPTESYEDFAASLPENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTAR 96

Query: 62  IRSKMLYATSKDRFRREWEGIHYEIQATDPTEMDLEVLRDRAN 100
           +RSKM+YA+SKDRF+RE +GI  E+QA DPTEM L+V++ RAN
Sbjct: 97  VRSKMIYASSKDRFKRELDGIQVELQACDPTEMGLDVIQSRAN 139

The following BLAST results are available for this feature:

BLAST of Lag0021105 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA8527768.1	1.5e-38	82.52	hypothetical protein F0562_035363 [Nyssa sinensis]	[more]
XP_023914794.1	1.5e-38	82.52	actin-depolymerizing factor [Quercus suber] >XP_030922753.1 actin-depolymerizing...	[more]
XP_028780695.1	1.5e-38	82.52	actin-depolymerizing factor-like [Prosopis alba] >XP_028780703.1 actin-depolymer...	[more]
XP_041001240.1	2.5e-38	82.52	actin-depolymerizing factor-like [Juglans microcarpa x Juglans regia]	[more]
THG07562.1	2.5e-38	81.55	hypothetical protein TEA_022966 [Camellia sinensis var. sinensis]	[more]

BLAST of Lag0021105 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8SAG3	2.0e-38	75.73	Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q2QLT8	9.3e-36	69.90	Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11...	[more]
Q9ZSK2	4.6e-35	70.59	Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1	[more]
Q9AY76	5.1e-34	67.96	Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 P...	[more]
Q9FVI2	5.3e-31	62.14	Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 1..99 e-value: 1.2E-23 score: 94.6
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 3..96 e-value: 4.0E-22 score: 78.3
IPR002108	Actin-depolymerising factor homology domain	PROSITE	PS51263	ADF_H	coord: 1..99 score: 21.320972
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	3.40.20.10	Severin	coord: 1..99 e-value: 1.8E-29 score: 104.4
IPR017904	ADF/Cofilin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 2..98
IPR017904	ADF/Cofilin	CDD	cd11286	ADF_cofilin_like	coord: 2..98 e-value: 1.0167E-39 score: 126.517
None	No IPR available	PANTHER	PTHR11913:SF96	COFILIN/ACTIN-DEPOLYMERIZING FACTOR-LIKE PROTEIN	coord: 2..98
None	No IPR available	SUPERFAMILY	55753	Actin depolymerizing proteins	coord: 3..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0021105.1	Lag0021105.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
molecular_function	GO:0003779	actin binding