Lag0020972 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0020972 vs. NCBI nr
Match: XP_022974983.1 (uncharacterized protein LOC111473852 isoform X1 [Cucurbita maxima] >XP_022974984.1 uncharacterized protein LOC111473852 isoform X1 [Cucurbita maxima] >XP_022974985.1 uncharacterized protein LOC111473852 isoform X2 [Cucurbita maxima])

HSP 1 Score: 788.5 bits (2035), Expect = 6.5e-224
Identity = 453/667 (67.92%), Postives = 506/667 (75.86%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQSQQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSVGF 60
           MYGRPS  +  GAGRR SQ QQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+S GF
Sbjct: 1   MYGRPSRFAPSGAGRRHSQPQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQSSGGF 60

Query: 61  IPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQPPK 120
           IPGHQNVNFP+QNSNLP QN GF F Q PN GFHS                    PQPP 
Sbjct: 61  IPGHQNVNFPMQNSNLPTQNHGFQFSQAPNPGFHS--------------------PQPPN 120

Query: 121 TGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGF 180
           TG Q H  T   AEG+ RI  AVAKAHSDLL A ES+SAWKVSQSALSILQVDSWDSLG 
Sbjct: 121 TGSQAHGKTTMPAEGVTRINNAVAKAHSDLLVAKESISAWKVSQSALSILQVDSWDSLGV 180

Query: 181 RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLR 240
           RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLGPFLR
Sbjct: 181 RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLGPFLR 240

Query: 241 HPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSVTK 300
           HPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKRS+ K
Sbjct: 241 HPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKRSIKK 300

Query: 301 RERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAI 360
           RE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEK RKRPLF +QKQQLD+R+ AI
Sbjct: 301 REMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKCRKRPLFKMQKQQLDERFSAI 360

Query: 361 SQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSD 420
           +QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK SLQ+++SSD
Sbjct: 361 AQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSLQKSRSSD 420

Query: 421 RSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGTPT 480
           R+SSCPYPSATEEMTRLGLKGEV GHAT IG QKH + + TSKRKRKSRDQSHTI G+ T
Sbjct: 421 RASSCPYPSATEEMTRLGLKGEVIGHATLIGSQKHIMNSGTSKRKRKSRDQSHTIFGSST 480

Query: 481 LGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEELAT- 540
            GKKVK EASI+DKDSF NSDEGS DEAEYKITNNS+RMFITTWKDGCRD+TV E L   
Sbjct: 481 SGKKVKVEASILDKDSFYNSDEGSGDEAEYKITNNSMRMFITTWKDGCRDMTVDEVLKRM 540

Query: 541 --KYGCK----------TGNWPNSYLGLPLNGSPRTL--SFWDPIVEKIEK-RLSVWGTS 600
              YG K            ++P  ++GL LN +  ++    WD I + ++   L     +
Sbjct: 541 LHHYGIKFRSRIRVRSMLSSYP--FIGL-LNVAVSSIKNGMWDSIYDSLQTINLHELSNT 600

Query: 601 YISKGGRHTLLQ-------ATLSNLPTYY--MSLFNMPNKVANKV---DRIFRN--FLWK 635
           ++ K   H  +        A + N+P +   +S+ ++ NKV   +     I  N  FL +
Sbjct: 601 HVEKQPVHERIDVGPSEEGAPIKNVPKFLHGISVEDILNKVNQHIKFEQEIHSNGWFLLE 644

BLAST of Lag0020972 vs. NCBI nr
Match: KAG6597285.1 (Protein NO VEIN, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 786.2 bits (2029), Expect = 3.2e-223
Identity = 429/541 (79.30%), Postives = 456/541 (84.29%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQS----QQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQT 60
           MYGRPS  +  GAGRRQS    QQQQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+
Sbjct: 1   MYGRPSRFAPSGAGRRQSQPQQQQQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQS 60

Query: 61  SVGFIPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVP 120
           S GFIPGHQNVNFP+QNSNLP QN GF F Q PN GFH          F Q  + GF  P
Sbjct: 61  SGGFIPGHQNVNFPMQNSNLPTQNHGFQFSQAPNPGFH----------FPQSARAGFHSP 120

Query: 121 QPPKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWD 180
           QPP T  Q H  TK   EG+ RI  AVA+AHSDLL A ES+SAWKVSQSALSILQVDSWD
Sbjct: 121 QPPNTSSQAHGKTKMPEEGVTRINNAVAEAHSDLLVAKESISAWKVSQSALSILQVDSWD 180

Query: 181 SLGFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLG 240
           SLG RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLG
Sbjct: 181 SLGVRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLG 240

Query: 241 PFLRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKR 300
           PFLRHPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKR
Sbjct: 241 PFLRHPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKR 300

Query: 301 SVTKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQR 360
           S+ KRE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEKRRKRPLF +QKQQLD+R
Sbjct: 301 SIKKREMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKRRKRPLFKMQKQQLDER 360

Query: 361 YGAISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRN 420
           + AI+QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK SLQ +
Sbjct: 361 FSAIAQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSLQNS 420

Query: 421 QSSDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTIL 480
           +SSDR+SSCPYPSATEEMTRLGLKGEV GHATPIGGQKH + + TSKRKRKSRDQSHTI 
Sbjct: 421 RSSDRASSCPYPSATEEMTRLGLKGEVIGHATPIGGQKHIMNSGTSKRKRKSRDQSHTIF 480

Query: 481 GTPTLGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEE 535
           G+ T  KKVK EASI DKDS  NSDEGS DEAEYKIT NS+RMFITTWKDGCRD+TV E 
Sbjct: 481 GSSTSSKKVKVEASIPDKDSLYNSDEGSGDEAEYKITYNSMRMFITTWKDGCRDMTVDEV 531

BLAST of Lag0020972 vs. NCBI nr
Match: XP_023539694.1 (uncharacterized protein LOC111800299 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.4 bits (2027), Expect = 5.5e-223
Identity = 428/537 (79.70%), Postives = 454/537 (84.54%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQSQQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSVGF 60
           MYGRPS  +  GAGRRQSQ QQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+S GF
Sbjct: 1   MYGRPSRFAPSGAGRRQSQPQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQSSGGF 60

Query: 61  IPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQPPK 120
           IPGHQNVNFP+QNSNLP QN GF F          SQ SNPG  F Q    GF  PQPP 
Sbjct: 61  IPGHQNVNFPMQNSNLPTQNHGFQF----------SQASNPGFHFPQSAHAGFHSPQPPN 120

Query: 121 TGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGF 180
           TG Q H  T   AEG+ RI  AVAKAHSDLL A ES+SAWKVSQSALSILQVDSWDSLG 
Sbjct: 121 TGSQAHGKTTMPAEGVTRINNAVAKAHSDLLVAKESISAWKVSQSALSILQVDSWDSLGV 180

Query: 181 RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLR 240
           RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLGPFLR
Sbjct: 181 RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLGPFLR 240

Query: 241 HPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSVTK 300
           HPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKRS+ K
Sbjct: 241 HPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKRSIKK 300

Query: 301 RERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAI 360
           RE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEK RKRPLF +QKQQLD+R+ AI
Sbjct: 301 REMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKCRKRPLFKMQKQQLDERFSAI 360

Query: 361 SQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSD 420
           +QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK S Q ++SSD
Sbjct: 361 AQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSQQNSRSSD 420

Query: 421 RSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGTPT 480
           R+SSCPYPSATEEMTRLGLKGEV GHATPIGGQKH + + TSKRKRKSRDQSHTI G+ T
Sbjct: 421 RASSCPYPSATEEMTRLGLKGEVIGHATPIGGQKHIMNSGTSKRKRKSRDQSHTIFGSST 480

Query: 481 LGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEEL 535
             KKVK EASI+DKDS  NSDEGS DEAEYKIT NS+RMFITTWKDGCRD+TV E L
Sbjct: 481 SSKKVKGEASILDKDSLYNSDEGSGDEAEYKITYNSMRMFITTWKDGCRDMTVDEVL 527

BLAST of Lag0020972 vs. NCBI nr
Match: XP_038891959.1 (protein NO VEIN isoform X2 [Benincasa hispida])

HSP 1 Score: 785.4 bits (2027), Expect = 5.5e-223
Identity = 423/544 (77.76%), Postives = 456/544 (83.82%), Query Frame = 0

Query: 1   MYGRPSSHPGAGRRQSQQQQQQPPPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSVGFIPG 60
           MYGRPSS    G  + Q QQQQPP   P+ QFPAAPY NP+V +QNLNH LQT +GF+P 
Sbjct: 1   MYGRPSSFGARGSGRRQPQQQQPP---PETQFPAAPYLNPDVAVQNLNHLLQTQIGFMP- 60

Query: 61  HQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQPPKTGF 120
           HQNVNFP+Q SNLPMQN GF F QP N G H  Q  NPG   SQ P PGFR PQP K G 
Sbjct: 61  HQNVNFPMQTSNLPMQNIGFQFSQPSNPGLHFPQAPNPGFHSSQSPNPGFRPPQPSKAGS 120

Query: 121 QPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGFRMQ 180
           QP SSTKK A GI  I   VAKAHSDLLAAGES+SAWKVSQSALSILQVD+WDSLG+RMQ
Sbjct: 121 QPQSSTKKLAGGILMINHTVAKAHSDLLAAGESISAWKVSQSALSILQVDTWDSLGYRMQ 180

Query: 181 EIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLRHPL 240
           EIDALHRL+ MEGKINSFIHCFVGVRRITTLYELEKAIC +EGV++FEELRLGPFLRHPL
Sbjct: 181 EIDALHRLMMMEGKINSFIHCFVGVRRITTLYELEKAICKDEGVRKFEELRLGPFLRHPL 240

Query: 241 VLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSVTKRER 300
           VLHYFSVNSEVTEV +IKSE+I+SFLS FMD D   NKNITVEDFLDFISRKRSVTKRE 
Sbjct: 241 VLHYFSVNSEVTEVCKIKSEDIISFLSEFMDQDVCSNKNITVEDFLDFISRKRSVTKREM 300

Query: 301 LGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAISQR 360
           LGIRVQNLG HISVIRKARRSEDDIL KYLT SDEKRRKRPLFSLQKQ+LD+R+ AISQR
Sbjct: 301 LGIRVQNLGTHISVIRKARRSEDDILSKYLTNSDEKRRKRPLFSLQKQKLDERFSAISQR 360

Query: 361 VESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSDRSS 420
           VESFS++HKDFCGKHVRF SSSSEDE  DDN +E KKN  + SS LKLSLQ N+SSDRSS
Sbjct: 361 VESFSSVHKDFCGKHVRFDSSSSEDEGCDDNAHEEKKNGTDLSSGLKLSLQSNKSSDRSS 420

Query: 421 SCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGTPTLGK 480
           SCPYPSATEEMTRLGLKGEVSGHATPIG Q HS+GN+TSKRKR+ R Q H   G+PTLGK
Sbjct: 421 SCPYPSATEEMTRLGLKGEVSGHATPIGSQNHSIGNSTSKRKRRYRHQRHNTSGSPTLGK 480

Query: 481 KVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAE---ELATK 540
           KVK E+SI+DKDSFDNSDEGS  EAEYKITNNSLRMFI TWKDGCRD+TVAE    +   
Sbjct: 481 KVKVESSILDKDSFDNSDEGSGVEAEYKITNNSLRMFIATWKDGCRDMTVAEVVKRMLDH 540

Query: 541 YGCK 542
           YG K
Sbjct: 541 YGIK 540

BLAST of Lag0020972 vs. NCBI nr
Match: KAG7028754.1 (hypothetical protein SDJN02_09935, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.5 bits (2022), Expect = 2.1e-222
Identity = 428/539 (79.41%), Postives = 454/539 (84.23%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQS--QQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSV 60
           MYGRPS  +  GAGRRQS  QQQQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+S 
Sbjct: 1   MYGRPSRFAPSGAGRRQSQPQQQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQSSG 60

Query: 61  GFIPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQP 120
           GFIPGHQNVNFP+QNSNLP QN GF F Q PN GFH          F Q    GF  PQP
Sbjct: 61  GFIPGHQNVNFPMQNSNLPTQNHGFQFSQAPNPGFH----------FPQSAHAGFHSPQP 120

Query: 121 PKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSL 180
           P T  Q H  T    EG+ RI  AVA+AHSDLL A ES+SAWKVSQSALSILQVDSWDSL
Sbjct: 121 PNTSSQAHGKTTMPEEGVTRINNAVAEAHSDLLVAKESISAWKVSQSALSILQVDSWDSL 180

Query: 181 GFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPF 240
           G RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLGPF
Sbjct: 181 GVRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLGPF 240

Query: 241 LRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSV 300
           LRHPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKRS+
Sbjct: 241 LRHPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKRSI 300

Query: 301 TKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYG 360
            KRE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEKRRKRPLF +QKQQLD+R+ 
Sbjct: 301 KKREMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKRRKRPLFKMQKQQLDERFS 360

Query: 361 AISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQS 420
           AI+QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK SLQ ++S
Sbjct: 361 AIAQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSLQNSRS 420

Query: 421 SDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGT 480
           SDR+SSCPYPSATEEMTRLGLKGEV GHATPIGGQKH + + TSKRKRKSRDQSHTI G+
Sbjct: 421 SDRASSCPYPSATEEMTRLGLKGEVIGHATPIGGQKHIMNSGTSKRKRKSRDQSHTIFGS 480

Query: 481 PTLGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEEL 535
            T  KKVK EASI DKDS  NSDEGS DEAEYKIT NS+RMFITTWKDGCRD+TV E L
Sbjct: 481 STSSKKVKVEASIPDKDSLYNSDEGSGDEAEYKITYNSMRMFITTWKDGCRDMTVDEVL 529

BLAST of Lag0020972 vs. ExPASy Swiss-Prot
Match: F4JTS8 (Protein NO VEIN OS=Arabidopsis thaliana OX=3702 GN=NOV PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 6.9e-67
Identity = 199/546 (36.45%), Postives = 288/546 (52.75%), Query Frame = 0

Query: 24  PPPQPQAQFPAAPYP-----NPNV-PLQNLN-------HHLQTSVG-FIPGHQNVNFPVQ 83
           P PQ  A F    +P     NPN  P QNLN       H   ++   F+  H   +F   
Sbjct: 54  PIPQFPANFYRPNFPDFSLGNPNFQPHQNLNFLHQQIPHQYGSAANHFLQNHNQNSFSFP 113

Query: 84  NSNLP------MQNQGFPFPQP-----------------PNSGFHSSQPSNPGSQFSQPP 143
             ++P       QN G  F                    P S F S + +N     S P 
Sbjct: 114 PQSIPNNDISISQNHG-AFENSSLKRRRQEEVVQVTDVVPKSNFASGESANNSFSVSLPI 173

Query: 144 KPGFRVPQPPKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSI 203
                     +   +  S   K    + RI KAV K     +AAGESVS+ +VS++ L  
Sbjct: 174 PIATDDSGVSRVHGEKSSGKPKRKVDVLRIDKAVNKTRKLFVAAGESVSSTRVSRAVLEE 233

Query: 204 LQVDSWDSLGFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQ 263
           LQ DSW SLG +MQ++ +L +L+A+EGKIN+FIHCFVG RRI TL++LE AIC NE V  
Sbjct: 234 LQADSWRSLGVQMQDVPSLRQLMAIEGKINAFIHCFVGARRIVTLHDLEVAICRNEFVDS 293

Query: 264 FEELRLGPFLRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFL 323
           F++L LGP L+HPLVL YF   S  T   +I SEEI+SFL +++       +++ +++FL
Sbjct: 294 FDDLELGPLLQHPLVLLYFPSISSSTGPVKITSEEIISFLDSYL--HTYMTEDVKLDEFL 353

Query: 324 DFISRKRSVTKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQ 383
           +F++ ++SVT +E+LG+R+Q+L M++S I  A+R E + L+  LT   E  +K  + S +
Sbjct: 354 NFVASQKSVTSKEKLGVRIQSLRMYVSFILDAKRQEGETLKVLLT---ELHQKYHIPSSK 413

Query: 384 KQQLDQRYGAISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDL 443
           KQQ D+    +S+R +SF+  HKD+CGKH+RF SSSS   D +DN YE            
Sbjct: 414 KQQRDKSL-TVSERADSFALHHKDYCGKHIRFDSSSS---DENDNVYE------------ 473

Query: 444 KLSLQRNQSSDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSR 503
              ++   SSD  +SCPYPS  EEM RLG            G  K   G    +R  +  
Sbjct: 474 ---VRNLNSSDHINSCPYPSVAEEMKRLG------------GSNKKRKG---ERRNHEKS 533

Query: 504 DQSHTILGTPT-LGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGC 532
           D S  +  +P+ L    K E   +  DS +     S DEA++ ++   LR+FI+TWKD C
Sbjct: 534 DSSKLLRKSPSKLQGHAKQEIPKLADDS-EAKKVFSVDEADFTLSEGDLRLFISTWKDTC 558

BLAST of Lag0020972 vs. ExPASy Swiss-Prot
Match: A0A1P8ARG1 (Protein NO VEIN-LIKE OS=Arabidopsis thaliana OX=3702 GN=NVL PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 9.6e-61
Identity = 155/409 (37.90%), Postives = 235/409 (57.46%), Query Frame = 0

Query: 133 IARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGFRMQEIDALHRLIAME 192
           + RI KAV      L+AAG++VS+ +VSQS L+ LQ D+W SLG  MQ++ +L +L+A+E
Sbjct: 70  LIRIDKAVNNTRKSLIAAGDNVSSTRVSQSVLAQLQADTWRSLGIWMQDVPSLRQLMALE 129

Query: 193 GKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLRHPLVLHYFSVNSEVT 252
           GKI +FIHCF+G R I TL++LE AIC NE V  F++L LGP L+HPLVL YF      T
Sbjct: 130 GKIIAFIHCFIGARGIVTLHDLEVAICQNEFVGCFDDLGLGPLLQHPLVLLYFPSVYCST 189

Query: 253 EVFEIKSEEIVSFLSAFM---DVDARRNKNITVEDFLDFISRKRSVTKRERLGIRVQNLG 312
              +I SEEI+S L +++   D+D     ++ +++FLDF++  ++VT +E+LG+R+QNL 
Sbjct: 190 APVKITSEEIISLLDSYLNTYDID-----DVKLDEFLDFVAEAKAVTSKEKLGVRIQNLR 249

Query: 313 MHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAISQRVESFSAIHK 372
           M++S I+ A+R E +IL+  LT+  +K R   + S +KQ+ D                 K
Sbjct: 250 MYVSFIQDAKRQEGEILKIVLTELHQKYR---ILSSKKQRQD-----------------K 309

Query: 373 DFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSDRSSSCPYPSATE 432
           D+CGKH RF S SSE+ DS D   EN K                  SD  SSCPY SA E
Sbjct: 310 DYCGKHTRFNSPSSEENDSADYEVENVKR-----------------SDHFSSCPYSSAEE 369

Query: 433 EMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGTPTL---GKKVKAEA 492
           E+ +LG                      +S +KRK+  ++H    +P L   G       
Sbjct: 370 EVKQLG----------------------SSSKKRKAESRNHEKSDSPKLLRRGPSKLRRG 414

Query: 493 SIVDK--DSFDNSDEG--SCDEAEYKITNNSLRMFITTWKDGCRDVTVA 532
            +  K   S D+SD    S ++A++ ++  +L++FI+TWK+ C++++++
Sbjct: 430 HVKQKIPKSADDSDAQIFSVNDADFTLSEGALKLFISTWKETCKELSIS 414

BLAST of Lag0020972 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 3.6e-31
Identity = 117/441 (26.53%), Postives = 194/441 (43.99%), Query Frame = 0

Query: 567 IVEKIEKRLSVWGTSYISKGGRHTLLQATLSNLPTYYMSLFNMPNKVANKVDRIFRNFLW 626
           I+E++  R+S W    +S  GR TL +A LS++P + MS   +P  + N++D++ R FLW
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 627 -----KDGQHLIKWSTLICPLKQGGLGLHSMRDKNRALLAKWVWRFNHEKTALWRKLLND 686
                K  QHL+KWS +  P K+GGLG+ + +  NRAL++K  WR   EK +LW  +L  
Sbjct: 76  GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 687 KY--GPSTYLNW----GSLNKAKGPWKAI-VKHQDLVTSRV-----RHQVVR---EAWNT 746
           KY  G      W    GS +     W++I +  +D+V+  V       Q +R   + W +
Sbjct: 136 KYHVGEIRDSRWLIPKGSWSST---WRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVS 195

Query: 747 NCSFWDLQLGRHLKDDEASEWMDI-------------------------NVILDPIVLAQ 806
                +L  G    D +     D+                          V+LD +  A+
Sbjct: 196 GKPLLELDNGERPTDCDTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVTGAR 255

Query: 807 TADSWIWTLSANGLFSTKS----LMVD---MGNTTTIFLENHLVRAIWKDIYPKKIKIFL 866
             D   W  S +G FS +S    L VD     N  + F        +WK   P+++K FL
Sbjct: 256 --DRLSWKFSQDGQFSVRSAYEMLTVDEVPRPNMASFF------NCLWKVRVPERVKTFL 315

Query: 867 WGLAHRAISTKDILQKRMPTLALSPSWCTLCKQSDETLDHLFIHCSYAWGIWTKILSSFG 926
           W + ++A+ T++   +R  +   + + C +CK   E++ H+   C    GIW +++    
Sbjct: 316 WLVGNQAVMTEEERHRRHLS---ASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQRR 375

Query: 927 WNLALTGDITSTLHSTLLGHPFKNAKVVLWLHIIRAFFWVLWKESNYRIFSDK-----EL 951
                +  +   L+  L        + + W  I     W  WK     IF +       +
Sbjct: 376 QQGFFSKSLFEWLYDNLGDR--SGCEDIPWSTIFAVIIWWGWKWRCGNIFGENTKCRDRV 435

BLAST of Lag0020972 vs. ExPASy TrEMBL
Match: A0A6J1IHX9 (uncharacterized protein LOC111473852 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111473852 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 3.2e-224
Identity = 453/667 (67.92%), Postives = 506/667 (75.86%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQSQQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSVGF 60
           MYGRPS  +  GAGRR SQ QQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+S GF
Sbjct: 1   MYGRPSRFAPSGAGRRHSQPQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQSSGGF 60

Query: 61  IPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQPPK 120
           IPGHQNVNFP+QNSNLP QN GF F Q PN GFHS                    PQPP 
Sbjct: 61  IPGHQNVNFPMQNSNLPTQNHGFQFSQAPNPGFHS--------------------PQPPN 120

Query: 121 TGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGF 180
           TG Q H  T   AEG+ RI  AVAKAHSDLL A ES+SAWKVSQSALSILQVDSWDSLG 
Sbjct: 121 TGSQAHGKTTMPAEGVTRINNAVAKAHSDLLVAKESISAWKVSQSALSILQVDSWDSLGV 180

Query: 181 RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLR 240
           RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLGPFLR
Sbjct: 181 RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLGPFLR 240

Query: 241 HPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSVTK 300
           HPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKRS+ K
Sbjct: 241 HPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKRSIKK 300

Query: 301 RERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAI 360
           RE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEK RKRPLF +QKQQLD+R+ AI
Sbjct: 301 REMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKCRKRPLFKMQKQQLDERFSAI 360

Query: 361 SQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSD 420
           +QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK SLQ+++SSD
Sbjct: 361 AQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSLQKSRSSD 420

Query: 421 RSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGTPT 480
           R+SSCPYPSATEEMTRLGLKGEV GHAT IG QKH + + TSKRKRKSRDQSHTI G+ T
Sbjct: 421 RASSCPYPSATEEMTRLGLKGEVIGHATLIGSQKHIMNSGTSKRKRKSRDQSHTIFGSST 480

Query: 481 LGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEELAT- 540
            GKKVK EASI+DKDSF NSDEGS DEAEYKITNNS+RMFITTWKDGCRD+TV E L   
Sbjct: 481 SGKKVKVEASILDKDSFYNSDEGSGDEAEYKITNNSMRMFITTWKDGCRDMTVDEVLKRM 540

Query: 541 --KYGCK----------TGNWPNSYLGLPLNGSPRTL--SFWDPIVEKIEK-RLSVWGTS 600
              YG K            ++P  ++GL LN +  ++    WD I + ++   L     +
Sbjct: 541 LHHYGIKFRSRIRVRSMLSSYP--FIGL-LNVAVSSIKNGMWDSIYDSLQTINLHELSNT 600

Query: 601 YISKGGRHTLLQ-------ATLSNLPTYY--MSLFNMPNKVANKV---DRIFRN--FLWK 635
           ++ K   H  +        A + N+P +   +S+ ++ NKV   +     I  N  FL +
Sbjct: 601 HVEKQPVHERIDVGPSEEGAPIKNVPKFLHGISVEDILNKVNQHIKFEQEIHSNGWFLLE 644

BLAST of Lag0020972 vs. ExPASy TrEMBL
Match: A0A6J1FQ82 (uncharacterized protein LOC111446266 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446266 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 3.9e-222
Identity = 427/539 (79.22%), Postives = 453/539 (84.04%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQS--QQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSV 60
           MYGRPS  +  GAGRRQS  QQQQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+S 
Sbjct: 1   MYGRPSRFAPSGAGRRQSQPQQQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQSSG 60

Query: 61  GFIPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQP 120
           GFIPGHQNVNFP+QNSNLP QN GF F Q PN GFH          F Q    GF  PQP
Sbjct: 61  GFIPGHQNVNFPMQNSNLPTQNHGFQFSQAPNPGFH----------FPQSAHAGFHSPQP 120

Query: 121 PKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSL 180
           P T  Q H  T    EG+ RI  AVA+AHSDLL A ES+SAWKVSQSALSILQVDSWDSL
Sbjct: 121 PNTSSQAHGKTTMPEEGVTRINNAVAEAHSDLLVAKESISAWKVSQSALSILQVDSWDSL 180

Query: 181 GFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPF 240
           G RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLGPF
Sbjct: 181 GVRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLGPF 240

Query: 241 LRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSV 300
           LRHPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKRS+
Sbjct: 241 LRHPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKRSI 300

Query: 301 TKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYG 360
            KRE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEKRRKRPLF +QKQQLD+R+ 
Sbjct: 301 KKREMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKRRKRPLFKMQKQQLDERFS 360

Query: 361 AISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQS 420
           AI+QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK SLQ ++S
Sbjct: 361 AIAQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSLQNSRS 420

Query: 421 SDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGT 480
           SDR+SSCPYPSATEEMTRLGLKGEV GHATPIGGQKH + + TSKRKRKSRDQ HTI G+
Sbjct: 421 SDRASSCPYPSATEEMTRLGLKGEVIGHATPIGGQKHIMNSGTSKRKRKSRDQRHTIFGS 480

Query: 481 PTLGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEEL 535
            T  KKVK EASI DKDS  NSDEGS DEAEYKIT NS+RMFITTWKDGCRD+TV E L
Sbjct: 481 STSSKKVKVEASIPDKDSLYNSDEGSGDEAEYKITYNSMRMFITTWKDGCRDMTVDEVL 529

BLAST of Lag0020972 vs. ExPASy TrEMBL
Match: A0A6J1FJE1 (uncharacterized protein LOC111446266 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446266 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 4.7e-220
Identity = 424/539 (78.66%), Postives = 450/539 (83.49%), Query Frame = 0

Query: 1   MYGRPS--SHPGAGRRQS--QQQQQQP-PPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSV 60
           MYGRPS  +  GAGRRQS  QQQQQQP P PQPQAQFPAAP+ NPNV L+ LNH LQ+S 
Sbjct: 1   MYGRPSRFAPSGAGRRQSQPQQQQQQPWPQPQPQAQFPAAPFFNPNVQLEALNHILQSSG 60

Query: 61  GFIPGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQP 120
           GFIPGHQNVNFP+QNSNLP QN GF F Q PN GFHS                    PQP
Sbjct: 61  GFIPGHQNVNFPMQNSNLPTQNHGFQFSQAPNPGFHS--------------------PQP 120

Query: 121 PKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSL 180
           P T  Q H  T    EG+ RI  AVA+AHSDLL A ES+SAWKVSQSALSILQVDSWDSL
Sbjct: 121 PNTSSQAHGKTTMPEEGVTRINNAVAEAHSDLLVAKESISAWKVSQSALSILQVDSWDSL 180

Query: 181 GFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPF 240
           G RMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAIC NEGV QFEELRLGPF
Sbjct: 181 GVRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICKNEGVNQFEELRLGPF 240

Query: 241 LRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSV 300
           LRHPLVLHYFSVNSEVTEVF IKSEEIV+ LS FMDVDA RNK+ITVEDFLDFISRKRS+
Sbjct: 241 LRHPLVLHYFSVNSEVTEVFNIKSEEIVTLLSKFMDVDACRNKDITVEDFLDFISRKRSI 300

Query: 301 TKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYG 360
            KRE LGIRVQNLGMHISVIRKARRSEDDIL KYLTKSDEKRRKRPLF +QKQQLD+R+ 
Sbjct: 301 KKREMLGIRVQNLGMHISVIRKARRSEDDILSKYLTKSDEKRRKRPLFKMQKQQLDERFS 360

Query: 361 AISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQS 420
           AI+QRVESFS++ KDFCGKHVRFASSSSEDEDSD NT ENKKN H+SSSDLK SLQ ++S
Sbjct: 361 AIAQRVESFSSVQKDFCGKHVRFASSSSEDEDSDVNTCENKKNGHDSSSDLKPSLQNSRS 420

Query: 421 SDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGT 480
           SDR+SSCPYPSATEEMTRLGLKGEV GHATPIGGQKH + + TSKRKRKSRDQ HTI G+
Sbjct: 421 SDRASSCPYPSATEEMTRLGLKGEVIGHATPIGGQKHIMNSGTSKRKRKSRDQRHTIFGS 480

Query: 481 PTLGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEEL 535
            T  KKVK EASI DKDS  NSDEGS DEAEYKIT NS+RMFITTWKDGCRD+TV E L
Sbjct: 481 STSSKKVKVEASIPDKDSLYNSDEGSGDEAEYKITYNSMRMFITTWKDGCRDMTVDEVL 519

BLAST of Lag0020972 vs. ExPASy TrEMBL
Match: A0A6J1CZA6 (uncharacterized protein LOC111016138 OS=Momordica charantia OX=3673 GN=LOC111016138 PE=4 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 3.9e-214
Identity = 427/605 (70.58%), Postives = 479/605 (79.17%), Query Frame = 0

Query: 1   MYGRPSSH--PGAGRRQSQQQQQQPPPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSVGFI 60
           MYGRPS    P AG R  QQQQ    PP PQAQFP APYPNPN PLQNLN+ LQ+ +GFI
Sbjct: 1   MYGRPSRFCPPSAGWRHPQQQQ----PPPPQAQFPPAPYPNPNDPLQNLNYLLQSHLGFI 60

Query: 61  PGHQNVNFPVQNSNLPMQNQGFPFPQPPNSGF--HSS--QPSNPGSQFSQPPKPGFRVPQ 120
           PGHQN+NFP+QNSNLPMQN GF FPQPPN GF  HSS  +P+   ++  +P KPGF+ PQ
Sbjct: 61  PGHQNLNFPMQNSNLPMQNPGFHFPQPPNPGFQPHSSVTEPAEVDARI-KPTKPGFQ-PQ 120

Query: 121 --------------PPKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVS 180
                          P  GFQP  S  + AE + RI  AV KAH DLL AGESVSAWKVS
Sbjct: 121 SSATKQAEVKARIKSPNQGFQPQVSATRPAEVMVRINHAVDKAHKDLLVAGESVSAWKVS 180

Query: 181 QSALSILQVDSWDSLGFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICT 240
           QS+LSILQ DSWD LG RMQEIDALHRLI +EGKINSFIHCFVGVRRITTLYELEKAIC 
Sbjct: 181 QSSLSILQADSWDYLGLRMQEIDALHRLIVVEGKINSFIHCFVGVRRITTLYELEKAICK 240

Query: 241 NEGVKQFEELRLGPFLRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNI 300
           NEGVKQFEELRLGPFLRHPLVLHYFSVNSEVTEVF+IKSEEIVSFLS F+DVDA RNKN 
Sbjct: 241 NEGVKQFEELRLGPFLRHPLVLHYFSVNSEVTEVFKIKSEEIVSFLSEFIDVDAGRNKNF 300

Query: 301 TVEDFLDFISRKRSVTKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKR 360
            VEDF+DFIS KRSVTKRE LG+RVQNLGMHISVI+ ARRSED+IL KYL+K DEKRRKR
Sbjct: 301 RVEDFMDFISGKRSVTKREMLGVRVQNLGMHISVIQNARRSEDEILSKYLSKPDEKRRKR 360

Query: 361 PLFSLQKQQLDQRYGAISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDH 420
           PLFSLQKQQLD+R+ AISQRVESFS++HKDFCGKHVRF SSSSEDEDS+DNT     N H
Sbjct: 361 PLFSLQKQQLDERFSAISQRVESFSSVHKDFCGKHVRFDSSSSEDEDSNDNT-----NSH 420

Query: 421 NSSSDLKLSLQRNQSSDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSK 480
           +SSS +KLSLQ N+SS+R+SSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHS+G  +SK
Sbjct: 421 DSSSHIKLSLQSNKSSERTSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSMGIGSSK 480

Query: 481 RKRKSRDQSHTILGTPTLGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITT 540
           RKRKSRDQSHT+ G+PTL KK K +ASI+DK S +N DEGS D+AEYKIT NSLR+FI T
Sbjct: 481 RKRKSRDQSHTVSGSPTLRKKPKIKASILDKVSINNFDEGSNDQAEYKITKNSLRIFIAT 540

Query: 541 WKDGCRDVTVAEELAT---KYGCK------TGNWPNSY--LGLPLNGSPRTL--SFWDPI 573
           WKDGCRD++VAE L      YG K       G+  +SY  +GL LN +  ++    WD I
Sbjct: 541 WKDGCRDLSVAEVLQRMLHHYGIKFRSKKRVGSMLSSYPFIGL-LNVAVSSIKNGMWDSI 593

BLAST of Lag0020972 vs. ExPASy TrEMBL
Match: A0A6J1E990 (uncharacterized protein LOC111431955 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431955 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 6.4e-209
Identity = 404/549 (73.59%), Postives = 446/549 (81.24%), Query Frame = 0

Query: 1   MYGRPSSHPGAGRRQSQQQQQQPPPPQPQAQFPAAPYPNPNVPLQNLNHHLQTSVGFIPG 60
           MYG PS+    G R+  +++Q P  P  QAQFPAAP+ NPNVP+QNLNH LQ    FIPG
Sbjct: 1   MYGGPSNFNNRGSRRRPRRRQNPQRPS-QAQFPAAPHLNPNVPVQNLNHPLQNRNVFIPG 60

Query: 61  HQNVNFPVQNSNLPMQNQGFPFPQPPNSGFHSSQPSNPGSQFSQPPKPGFRVPQPPKTGF 120
           HQNVNFP+QNSNLP QN GF                    QFSQPP PGFR  QP +TGF
Sbjct: 61  HQNVNFPMQNSNLPEQNLGF--------------------QFSQPPSPGFRFRQPSRTGF 120

Query: 121 QPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGFRMQ 180
           QP SST KSAEGIARI  AVAKAHSDLL  GES+SAWKVSQSALSILQVDSW+ LGFRMQ
Sbjct: 121 QPQSSTTKSAEGIARINHAVAKAHSDLLVGGESISAWKVSQSALSILQVDSWEYLGFRMQ 180

Query: 181 EIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLRHPL 240
           E+DALHRLI MEGKINSFIHCFVGVRRITTLYELEKAIC +E VKQFEELRLGPFLRHPL
Sbjct: 181 EVDALHRLIVMEGKINSFIHCFVGVRRITTLYELEKAICKDERVKQFEELRLGPFLRHPL 240

Query: 241 VLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFLDFISRKRSVTKRER 300
           VLHYFSVNSEV EVF+IKSEEI+ +L  FMDVDA RNKNI VEDFLDFISRKRSVTK+E 
Sbjct: 241 VLHYFSVNSEVNEVFKIKSEEIICYLLEFMDVDACRNKNIMVEDFLDFISRKRSVTKKEM 300

Query: 301 LGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAISQR 360
           LGIRVQNL  HISVIRKARRSEDDIL KYLTKS+EKRRKRPLFS++KQQLD+R+ A+SQR
Sbjct: 301 LGIRVQNLETHISVIRKARRSEDDILSKYLTKSNEKRRKRPLFSMKKQQLDKRFSAVSQR 360

Query: 361 VESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSDRSS 420
           VES S++ KD+CGKHVRF+S SS DEDSDDNT++ +KN ++SSSDLK+ LQ N+S D SS
Sbjct: 361 VESSSSVPKDYCGKHVRFSSESSGDEDSDDNTHKTEKNGNHSSSDLKVPLQINKSYDSSS 420

Query: 421 SCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATS--KRKRKSRDQSHTILGTPTL 480
           SCPYPSATEE+ RLGL GEVS HA+PIG QK S+GN TS  KRKRKS  Q HT  G+PTL
Sbjct: 421 SCPYPSATEEVARLGLNGEVSVHASPIGSQKDSVGNGTSKRKRKRKSGHQMHTTFGSPTL 480

Query: 481 GKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGCRDVTVAEELAT-- 540
           GKKVK EAS+++KDSFDNSDEGS DEAE KITNNSLRMFITTWKDGCRD TV E L    
Sbjct: 481 GKKVKVEASLLEKDSFDNSDEGSGDEAECKITNNSLRMFITTWKDGCRDATVPEVLKRMF 528

Query: 541 -KYGCKTGN 545
             YG K G+
Sbjct: 541 HHYGIKLGS 528

BLAST of Lag0020972 vs. TAIR 10
Match: AT4G13750.1 (Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase family protein )

HSP 1 Score: 257.3 bits (656), Expect = 4.9e-68
Identity = 199/546 (36.45%), Postives = 288/546 (52.75%), Query Frame = 0

Query: 24  PPPQPQAQFPAAPYP-----NPNV-PLQNLN-------HHLQTSVG-FIPGHQNVNFPVQ 83
           P PQ  A F    +P     NPN  P QNLN       H   ++   F+  H   +F   
Sbjct: 54  PIPQFPANFYRPNFPDFSLGNPNFQPHQNLNFLHQQIPHQYGSAANHFLQNHNQNSFSFP 113

Query: 84  NSNLP------MQNQGFPFPQP-----------------PNSGFHSSQPSNPGSQFSQPP 143
             ++P       QN G  F                    P S F S + +N     S P 
Sbjct: 114 PQSIPNNDISISQNHG-AFENSSLKRRRQEEVVQVTDVVPKSNFASGESANNSFSVSLPI 173

Query: 144 KPGFRVPQPPKTGFQPHSSTKKSAEGIARIKKAVAKAHSDLLAAGESVSAWKVSQSALSI 203
                     +   +  S   K    + RI KAV K     +AAGESVS+ +VS++ L  
Sbjct: 174 PIATDDSGVSRVHGEKSSGKPKRKVDVLRIDKAVNKTRKLFVAAGESVSSTRVSRAVLEE 233

Query: 204 LQVDSWDSLGFRMQEIDALHRLIAMEGKINSFIHCFVGVRRITTLYELEKAICTNEGVKQ 263
           LQ DSW SLG +MQ++ +L +L+A+EGKIN+FIHCFVG RRI TL++LE AIC NE V  
Sbjct: 234 LQADSWRSLGVQMQDVPSLRQLMAIEGKINAFIHCFVGARRIVTLHDLEVAICRNEFVDS 293

Query: 264 FEELRLGPFLRHPLVLHYFSVNSEVTEVFEIKSEEIVSFLSAFMDVDARRNKNITVEDFL 323
           F++L LGP L+HPLVL YF   S  T   +I SEEI+SFL +++       +++ +++FL
Sbjct: 294 FDDLELGPLLQHPLVLLYFPSISSSTGPVKITSEEIISFLDSYL--HTYMTEDVKLDEFL 353

Query: 324 DFISRKRSVTKRERLGIRVQNLGMHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQ 383
           +F++ ++SVT +E+LG+R+Q+L M++S I  A+R E + L+  LT   E  +K  + S +
Sbjct: 354 NFVASQKSVTSKEKLGVRIQSLRMYVSFILDAKRQEGETLKVLLT---ELHQKYHIPSSK 413

Query: 384 KQQLDQRYGAISQRVESFSAIHKDFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDL 443
           KQQ D+    +S+R +SF+  HKD+CGKH+RF SSSS   D +DN YE            
Sbjct: 414 KQQRDKSL-TVSERADSFALHHKDYCGKHIRFDSSSS---DENDNVYE------------ 473

Query: 444 KLSLQRNQSSDRSSSCPYPSATEEMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSR 503
              ++   SSD  +SCPYPS  EEM RLG            G  K   G    +R  +  
Sbjct: 474 ---VRNLNSSDHINSCPYPSVAEEMKRLG------------GSNKKRKG---ERRNHEKS 533

Query: 504 DQSHTILGTPT-LGKKVKAEASIVDKDSFDNSDEGSCDEAEYKITNNSLRMFITTWKDGC 532
           D S  +  +P+ L    K E   +  DS +     S DEA++ ++   LR+FI+TWKD C
Sbjct: 534 DSSKLLRKSPSKLQGHAKQEIPKLADDS-EAKKVFSVDEADFTLSEGDLRLFISTWKDTC 558

BLAST of Lag0020972 vs. TAIR 10
Match: AT1G08300.1 (no vein-like )

HSP 1 Score: 236.9 bits (603), Expect = 6.8e-62
Identity = 155/409 (37.90%), Postives = 235/409 (57.46%), Query Frame = 0

Query: 133 IARIKKAVAKAHSDLLAAGESVSAWKVSQSALSILQVDSWDSLGFRMQEIDALHRLIAME 192
           + RI KAV      L+AAG++VS+ +VSQS L+ LQ D+W SLG  MQ++ +L +L+A+E
Sbjct: 124 LIRIDKAVNNTRKSLIAAGDNVSSTRVSQSVLAQLQADTWRSLGIWMQDVPSLRQLMALE 183

Query: 193 GKINSFIHCFVGVRRITTLYELEKAICTNEGVKQFEELRLGPFLRHPLVLHYFSVNSEVT 252
           GKI +FIHCF+G R I TL++LE AIC NE V  F++L LGP L+HPLVL YF      T
Sbjct: 184 GKIIAFIHCFIGARGIVTLHDLEVAICQNEFVGCFDDLGLGPLLQHPLVLLYFPSVYCST 243

Query: 253 EVFEIKSEEIVSFLSAFM---DVDARRNKNITVEDFLDFISRKRSVTKRERLGIRVQNLG 312
              +I SEEI+S L +++   D+D     ++ +++FLDF++  ++VT +E+LG+R+QNL 
Sbjct: 244 APVKITSEEIISLLDSYLNTYDID-----DVKLDEFLDFVAEAKAVTSKEKLGVRIQNLR 303

Query: 313 MHISVIRKARRSEDDILRKYLTKSDEKRRKRPLFSLQKQQLDQRYGAISQRVESFSAIHK 372
           M++S I+ A+R E +IL+  LT+  +K R   + S +KQ+ D                 K
Sbjct: 304 MYVSFIQDAKRQEGEILKIVLTELHQKYR---ILSSKKQRQD-----------------K 363

Query: 373 DFCGKHVRFASSSSEDEDSDDNTYENKKNDHNSSSDLKLSLQRNQSSDRSSSCPYPSATE 432
           D+CGKH RF S SSE+ DS D   EN K                  SD  SSCPY SA E
Sbjct: 364 DYCGKHTRFNSPSSEENDSADYEVENVKR-----------------SDHFSSCPYSSAEE 423

Query: 433 EMTRLGLKGEVSGHATPIGGQKHSLGNATSKRKRKSRDQSHTILGTPTL---GKKVKAEA 492
           E+ +LG                      +S +KRK+  ++H    +P L   G       
Sbjct: 424 EVKQLG----------------------SSSKKRKAESRNHEKSDSPKLLRRGPSKLRRG 468

Query: 493 SIVDK--DSFDNSDEG--SCDEAEYKITNNSLRMFITTWKDGCRDVTVA 532
            +  K   S D+SD    S ++A++ ++  +L++FI+TWK+ C++++++
Sbjct: 484 HVKQKIPKSADDSDAQIFSVNDADFTLSEGALKLFISTWKETCKELSIS 468

BLAST of Lag0020972 vs. TAIR 10
Match: AT1G45063.1 (copper ion binding;electron carriers )

HSP 1 Score: 50.4 bits (119), Expect = 9.1e-06
Identity = 30/93 (32.26%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 839 PSWCTLCKQSDETLDHLFIHCSYAWGIWTKILSSFGWNLALTGDITSTLHSTLLGHPFKN 898
           PS C LC   DET  H+F  C ++  +W    S F  N  +T        +  L HP ++
Sbjct: 281 PSLCLLCNALDETRQHVFFDCPFSHEVW----SFFCSNARVTPPRMFKDSARWLRHPCRD 340

Query: 899 AKVVLWLHI-IRAFFWVLWKESNYRIFSDKELP 931
            KV   L +  +A  + +W+E N R+ S+K  P
Sbjct: 341 KKVAFILKLAYQASVYHIWRERNIRLNSNKSFP 369