Lag0020965 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0020965 vs. NCBI nr
Match: KGN56746.2 (hypothetical protein Csa_011800 [Cucumis sativus])

HSP 1 Score: 963.8 bits (2490), Expect = 8.6e-277
Identity = 523/726 (72.04%), Postives = 569/726 (78.37%), Query Frame = 0

Query: 1   MSIHSTNDIREWNAGVVERRTVMDFDLNCPPPDECIDPTVPHEEAAQYYNRYPGQAMDNP 60
           MSI S+ND+ +W++ V+ERRTV+DFDLNCPPPDECIDPT   +EAAQYYN Y GQA D  
Sbjct: 1   MSIQSSNDLHDWSSRVIERRTVLDFDLNCPPPDECIDPTGLPDEAAQYYNHYQGQATD-- 60

Query: 61  EFVDEDIAIISPRKFAEARKNFRRNHFESSCGVAVRRNGNTEVYGSLSDVTSWPPFTIWP 120
             +DEDIAIISPRKFAEARKNFRRNHFES CG  +RRNGNTEVYG+LSDVT+WPPFTIW 
Sbjct: 61  -AIDEDIAIISPRKFAEARKNFRRNHFESGCGAVIRRNGNTEVYGALSDVTTWPPFTIWS 120

Query: 121 PLTISNNVSIQE-QAIHNLDLCLSCESSSRAIKA-TDTDIPSALAQNSSIPPAAPSLRCA 180
           PLTISNNVS+QE Q IHNLDL LSCESSSRA KA TDTDIPS LA +SSIPP   +LRCA
Sbjct: 121 PLTISNNVSLQEQQTIHNLDLRLSCESSSRATKAKTDTDIPSTLALSSSIPPTDRTLRCA 180

Query: 181 ICIEPLVEETTTKCGHVFCRNCIETAIATQHRCPICRRFGFGFYNAFLSSYSKDISVSQP 240
           ICIEPLVEETTTKCGH                                            
Sbjct: 181 ICIEPLVEETTTKCGHT------------------------------------------- 240

Query: 241 EGVSNWPASIDLAFVFLVHHAGKESENRWLCLYLFCLFSSLSFIKAAFEVDFGGHQVRFC 300
                                   +EN+ L                           RF 
Sbjct: 241 ----------------------NNAENQNLS--------------------------RFR 300

Query: 301 ANSGQRKEKTMPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKL 360
           ANSG+ + K MPESMEATPSV PSLDLQAVR   SELEEL RSLEE+E STTDSLGSEKL
Sbjct: 301 ANSGRIRRKMMPESMEATPSVPPSLDLQAVR---SELEELQRSLEENEESTTDSLGSEKL 360

Query: 361 LKECAVHLESRLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLK 420
           L+ECA+HLESR+QQVLSE SNV+SFLGIDDLDAYVE MKEELV VEAESSKISNEIEVLK
Sbjct: 361 LRECALHLESRIQQVLSEYSNVDSFLGIDDLDAYVEHMKEELVAVEAESSKISNEIEVLK 420

Query: 421 RTNMEDSNKLKMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVL 480
           RTN+EDSNKLKMDLEVLK S+D  F SQDPE+ATFNC S+NGED MN+IV RECNAFEVL
Sbjct: 421 RTNIEDSNKLKMDLEVLKLSLD-RFPSQDPEEATFNCSSMNGEDPMNVIVNRECNAFEVL 480

Query: 481 ELDSQIEKNKRILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNL 540
           EL+SQIEKNK+ILKSLQE+DEIFKSLDVIEQVE TIGG+KVIDVADN IRLSLHTHIPN+
Sbjct: 481 ELESQIEKNKKILKSLQEVDEIFKSLDVIEQVEGTIGGMKVIDVADNSIRLSLHTHIPNV 540

Query: 541 EYFSSLQRLEGMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSL 600
           E FS+LQRLEG+IE SELDHEL+IEVL+GTMELKNAEIFP DVHLHDIINASKS  NSSL
Sbjct: 541 EDFSTLQRLEGLIEKSELDHELIIEVLDGTMELKNAEIFPADVHLHDIINASKSISNSSL 600

Query: 601 EWFVRKVQDRIVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPL 660
           EWFVRKVQDRIVLCTLRR  VKSANKS HSFEYLDQDE I+C+MIGGIDA IKV QGWPL
Sbjct: 601 EWFVRKVQDRIVLCTLRRFAVKSANKSCHSFEYLDQDEMIMCSMIGGIDACIKVSQGWPL 628

Query: 661 SDSPLKLISLKNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHL 720
           +DSPLKLISLK+SDHYTKGVSLSLICKVEKMANSLDA +R+NLS+FADA+EKILKEQMHL
Sbjct: 661 ADSPLKLISLKSSDHYTKGVSLSLICKVEKMANSLDAHIRRNLSSFADAVEKILKEQMHL 628

Query: 721 ELQADS 725
           ELQADS
Sbjct: 721 ELQADS 628

BLAST of Lag0020965 vs. NCBI nr
Match: KAG7018870.1 (hypothetical protein SDJN02_20743, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 886.7 bits (2290), Expect = 1.3e-253
Identity = 501/738 (67.89%), Postives = 552/738 (74.80%), Query Frame = 0

Query: 14  AGVVERRTVMDFDLNCPPPDECIDPTVPHEEAAQYYNRYPGQAMDNPEFVDEDIAIISPR 73
           A VVERRT +D DLNCPPPDECIDPT PH+EAAQY N +  QA D    VDEDIAIISPR
Sbjct: 76  ARVVERRTRLDIDLNCPPPDECIDPTGPHDEAAQYNNHHREQATD---AVDEDIAIISPR 135

Query: 74  KFAEARKNFRRNHFESSCGVAVRRNGNTEVYGSLSDVTSWPPFTIWPPLTISNNVSIQE- 133
           KFAEARKNFRRNHFESS GV VRRNG+TEVY +L+DV+SWPPFTIW P T  N+VSIQE 
Sbjct: 136 KFAEARKNFRRNHFESSYGVVVRRNGSTEVYSALTDVSSWPPFTIWSPRTNRNSVSIQEQ 195

Query: 134 QAIHNLDLCLSCESSSRAIKA-TDTDIPSALAQNSSIPPAAPSLRCAICIEPLVEETTTK 193
           Q  HNLDL LSCE+SSRA K  TD  IPSA A NSSI PA   LRCAICIEPLVEETTTK
Sbjct: 196 QTTHNLDLRLSCETSSRATKVKTDIVIPSAPALNSSITPADRKLRCAICIEPLVEETTTK 255

Query: 194 CGHVFCRNCIETAIATQHRCPICRRFGFGFYNAFLSSYSKDISVSQPEGVSNWPASIDLA 253
           CGH+FCRNCIE AIATQH+CPICRR                        +  W +S    
Sbjct: 256 CGHIFCRNCIEIAIATQHKCPICRR------------------------LYEWLSS---- 315

Query: 254 FVFLVHHAGKESENRWLCLYLFCLFSSLSFIKAAFEVDFGGHQVRFCANSGQRKEKTMPE 313
              L  H G ES+ R +   +                           + G  +   + E
Sbjct: 316 --SLFIHGGNESQTRGITRGI---------------------------SLGSVRIPAVDE 375

Query: 314 SMEATPSVSPSLDLQAVRS-RISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLESRL 373
              A    S ++ +   RS   S+LEEL RSL EDE+ +TDSLGSEKLLKECA+HLESRL
Sbjct: 376 ENNAEIDGSYTVCVFKPRSPSSSQLEELQRSLAEDEAYSTDSLGSEKLLKECALHLESRL 435

Query: 374 QQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIE------VLKRTNMED 433
           QQVLSECSNV+SFLGIDDLDAYVE MKEELV VEAESSKISNEIE      VL    +  
Sbjct: 436 QQVLSECSNVDSFLGIDDLDAYVEHMKEELVAVEAESSKISNEIEPPICIDVLLLLPITG 495

Query: 434 SNKLKMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQI 493
           SNKL++DLE+L  S+D  FTSQD EK TFN CS+NGEDQMN+IV+ ECNAFEVLELDS I
Sbjct: 496 SNKLEVDLELLDVSLD-RFTSQDTEKETFNFCSMNGEDQMNVIVDCECNAFEVLELDSHI 555

Query: 494 EKNKRILKSLQELDEIFKSL----------------DVIEQVEDTIGGLKVIDVADNFIR 553
           EKNKRILKSLQE+DEIFKS+                +  + VEDTIGGLKVI VADNFIR
Sbjct: 556 EKNKRILKSLQEVDEIFKSITAPSCSALFLPFLCSAEPHDSVEDTIGGLKVIGVADNFIR 615

Query: 554 LSLHTHIPNLEYFSSLQRLEGMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIIN 613
           LSL THIPNLE FSSLQRLEGMIEPSEL+HELLIEVLEGTMELKNAEIFPGDVHLHDIIN
Sbjct: 616 LSLRTHIPNLEDFSSLQRLEGMIEPSELNHELLIEVLEGTMELKNAEIFPGDVHLHDIIN 675

Query: 614 ASKSFRNSSLEWFVRKVQDRIVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDA 673
           ASKS  N SLEWFV+KVQDRIVLCTLRR VVKSANKSSHSF+Y+DQDETI+C MIGGIDA
Sbjct: 676 ASKSVSN-SLEWFVKKVQDRIVLCTLRRFVVKSANKSSHSFDYIDQDETIVCCMIGGIDA 735

Query: 674 LIKVFQGWPLSDSPLKLISLKNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAI 727
            IKV QGWPL+DSPLKL+SLK+SDHYTKG SLSL+CKVEKMANSLDAR+RQNLS+FADA+
Sbjct: 736 FIKVSQGWPLADSPLKLVSLKSSDHYTKGASLSLVCKVEKMANSLDARIRQNLSSFADAV 751

BLAST of Lag0020965 vs. NCBI nr
Match: XP_023528067.1 (uncharacterized protein LOC111791098 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528069.1 uncharacterized protein LOC111791098 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.5 bits (1804), Expect = 3.0e-197
Identity = 367/421 (87.17%), Postives = 394/421 (93.59%), Query Frame = 0

Query: 306 EKTMPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVH 365
           EKTMPESMEATPSVS SLDLQAVRSRISELEEL RSLEEDE+  TDSLGSEKLLKECA+H
Sbjct: 2   EKTMPESMEATPSVSSSLDLQAVRSRISELEELQRSLEEDEAYCTDSLGSEKLLKECALH 61

Query: 366 LESRLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDS 425
           LESRLQQVLSECSNV+SFL IDDLDAYVE MKEELV VEAESSKISNEIEVLKRTN+E S
Sbjct: 62  LESRLQQVLSECSNVDSFLRIDDLDAYVEHMKEELVAVEAESSKISNEIEVLKRTNIEGS 121

Query: 426 NKLKMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIE 485
           NKL++DLE+L  S+D  FTSQDPE  TFN CS+NGEDQMN+IV+ ECNAFEVLELDS IE
Sbjct: 122 NKLEVDLELLNVSLD-RFTSQDPENETFNFCSMNGEDQMNVIVDPECNAFEVLELDSHIE 181

Query: 486 KNKRILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQ 545
           KNKRILKSLQE+DEIFKSLDV+EQVEDTIGGLKVIDVADNFIRLSL THIPNLE FSSLQ
Sbjct: 182 KNKRILKSLQEVDEIFKSLDVVEQVEDTIGGLKVIDVADNFIRLSLRTHIPNLEDFSSLQ 241

Query: 546 RLEGMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKV 605
           +LEGMIEPSEL+HELLIEVLEGTMELKNAEIFPGDVHLHDIINASKS  N SLEWFV+KV
Sbjct: 242 KLEGMIEPSELNHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSVSN-SLEWFVKKV 301

Query: 606 QDRIVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKL 665
           QDRIVLCTLRR VVKSANKSSHSF+Y+DQDETI+C+MIGGIDA IKV QGWPL+DSPLKL
Sbjct: 302 QDRIVLCTLRRFVVKSANKSSHSFDYIDQDETIVCSMIGGIDAFIKVSQGWPLADSPLKL 361

Query: 666 ISLKNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSA 725
           +SLK+SDHYTKG SLSL+CKVEKMANSLDAR+RQNLS+FADA+EKILKEQMHLELQADS 
Sbjct: 362 VSLKSSDHYTKGASLSLVCKVEKMANSLDARIRQNLSSFADAVEKILKEQMHLELQADSG 420

Query: 726 L 727
           L
Sbjct: 422 L 420

BLAST of Lag0020965 vs. NCBI nr
Match: XP_022147070.1 (uncharacterized protein LOC111016098 [Momordica charantia])

HSP 1 Score: 695.3 bits (1793), Expect = 5.7e-196
Identity = 364/418 (87.08%), Postives = 391/418 (93.54%), Query Frame = 0

Query: 309 MPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLES 368
           MPESMEATPSV P LDLQAVR RISELEEL RSLEEDE+S+TDSLGSEKLLKECA+HLES
Sbjct: 1   MPESMEATPSVPPILDLQAVRCRISELEELQRSLEEDEASSTDSLGSEKLLKECALHLES 60

Query: 369 RLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKL 428
           RLQQ+LSE SNV+SFLGIDDLDAYVE MKEELVMVEAESSKISNEIE +KR N+EDSN+L
Sbjct: 61  RLQQILSEFSNVDSFLGIDDLDAYVEHMKEELVMVEAESSKISNEIEAIKRNNIEDSNRL 120

Query: 429 KMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNK 488
           KMDLEVLK S+D   TS+DPEKATFNC S +GEDQ+NMI +RECNAFEVL+LDSQIE+NK
Sbjct: 121 KMDLEVLKLSLD-RLTSEDPEKATFNCRSPDGEDQINMIDKRECNAFEVLQLDSQIEQNK 180

Query: 489 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLE 548
           RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVA+NFIRLSL THIPNLE  SSLQRLE
Sbjct: 181 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVAENFIRLSLRTHIPNLEDLSSLQRLE 240

Query: 549 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKVQDR 608
           G+IEPSEL+HELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSF NSSLEWFVRKVQDR
Sbjct: 241 GVIEPSELNHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFSNSSLEWFVRKVQDR 300

Query: 609 IVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISL 668
           IVLCTLRR VVK+ANKSSHSFEYLD+D TIICTMIGGI A+I+V QGWPLSDSPLKLISL
Sbjct: 301 IVLCTLRRFVVKTANKSSHSFEYLDEDNTIICTMIGGIGAVIRVSQGWPLSDSPLKLISL 360

Query: 669 KNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSAL 727
           KNSDHYTKG+SLSLICKVEKMANSLD R+R NLS+FADAIEKILKE+MHLEL +D AL
Sbjct: 361 KNSDHYTKGISLSLICKVEKMANSLDVRIRHNLSSFADAIEKILKERMHLELHSDGAL 417

BLAST of Lag0020965 vs. NCBI nr
Match: XP_023538691.1 (uncharacterized protein LOC111799561 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693.7 bits (1789), Expect = 1.7e-195
Identity = 366/418 (87.56%), Postives = 390/418 (93.30%), Query Frame = 0

Query: 309 MPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLES 368
           M E MEATPSVSPS+D+QAVRS ISE+EEL RSLEEDE+ TTDSLGSEKLLKEC++ LES
Sbjct: 1   MAELMEATPSVSPSVDVQAVRSCISEIEELQRSLEEDEAYTTDSLGSEKLLKECSLLLES 60

Query: 369 RLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKL 428
           RLQQ LSE SNV+SFLGIDDLDAYVERMKEEL+ VEAESSKIS+EIEVLKRT++EDSNKL
Sbjct: 61  RLQQTLSEWSNVDSFLGIDDLDAYVERMKEELIAVEAESSKISSEIEVLKRTSIEDSNKL 120

Query: 429 KMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNK 488
           +MDLEVLK S+D    SQDPEKATFNC SVNGEDQM+MIVERECNAFEVLELDSQIEKN+
Sbjct: 121 RMDLEVLKLSLD-RCASQDPEKATFNCYSVNGEDQMDMIVERECNAFEVLELDSQIEKNE 180

Query: 489 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLE 548
           R LKSLQELDEIFKSLDVIEQVEDTIGGLKVIDV DNF+R+SLH+HIPNLE FSSLQRLE
Sbjct: 181 RTLKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVTDNFVRVSLHSHIPNLEEFSSLQRLE 240

Query: 549 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKVQDR 608
           GMIEPSELDHELLIEVLEGTMEL NAEIFPGDVHLHDIINASKSF NSSLEWFVRKVQDR
Sbjct: 241 GMIEPSELDHELLIEVLEGTMELTNAEIFPGDVHLHDIINASKSFSNSSLEWFVRKVQDR 300

Query: 609 IVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISL 668
           IVLC LRR VVKSANKSSHSFEYLDQDETIICTMIGGIDA+IKV QGWPLSDSPLKLISL
Sbjct: 301 IVLCALRRFVVKSANKSSHSFEYLDQDETIICTMIGGIDAVIKVSQGWPLSDSPLKLISL 360

Query: 669 KNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSAL 727
           K+SDHY KGVSLSLICKVEKMANSLD  +R NLS+FADA+EKI+KEQMHLELQ DSAL
Sbjct: 361 KSSDHYIKGVSLSLICKVEKMANSLDVGLRHNLSSFADAVEKIMKEQMHLELQFDSAL 417

BLAST of Lag0020965 vs. ExPASy Swiss-Prot
Match: Q95KF1 (E3 ubiquitin-protein ligase RNF125 OS=Macaca fascicularis OX=9541 GN=RNF125 PE=2 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 1.9e-05
Identity = 27/73 (36.99%), Postives = 38/73 (52.05%), Query Frame = 0

Query: 146 SSSRAIKATDTDIPSALAQNSSIPPAAPSLRCAICIEPLVEETTTKCGHVFCRNCIETAI 205
           SS     A  +  P AL +         S  CA+C+E L +   T+CGHVFCR+CI T++
Sbjct: 6   SSDSGKSAPPSATPRALERRGDPELPVTSFDCAVCLEVLHQPVRTRCGHVFCRSCIATSL 65

Query: 206 A-TQHRCPICRRF 218
              +  CP CR +
Sbjct: 66  KNNKWTCPYCRAY 78

BLAST of Lag0020965 vs. ExPASy Swiss-Prot
Match: Q9D9R0 (E3 ubiquitin-protein ligase RNF125 OS=Mus musculus OX=10090 GN=Rnf125 PE=2 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 1.9e-05
Identity = 28/78 (35.90%), Postives = 43/78 (55.13%), Query Frame = 0

Query: 142 LSCESSSRAIKATDTDIPSALAQNSSIPPAAPSLRCAICIEPLVEETTTKCGHVFCRNCI 201
           L    SS++  A+ T  P  L ++        S  C++C+E L +   T+CGHVFCR+CI
Sbjct: 4   LLSSDSSKSAPASAT--PRTLERSGDSELPITSFDCSVCLEVLHQPVRTRCGHVFCRSCI 63

Query: 202 ETAIATQHR--CPICRRF 218
            T+I   ++  CP CR +
Sbjct: 64  ATSIKNNNKWTCPYCRAY 79

BLAST of Lag0020965 vs. ExPASy Swiss-Prot
Match: Q96EQ8 (E3 ubiquitin-protein ligase RNF125 OS=Homo sapiens OX=9606 GN=RNF125 PE=1 SV=4)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-05
Identity = 21/45 (46.67%), Postives = 30/45 (66.67%), Query Frame = 0

Query: 174 SLRCAICIEPLVEETTTKCGHVFCRNCIETAIA-TQHRCPICRRF 218
           S  CA+C+E L +   T+CGHVFCR+CI T++   +  CP CR +
Sbjct: 34  SFDCAVCLEVLHQPVRTRCGHVFCRSCIATSLKNNKWTCPYCRAY 78

BLAST of Lag0020965 vs. ExPASy Swiss-Prot
Match: Q14258 (E3 ubiquitin/ISG15 ligase TRIM25 OS=Homo sapiens OX=9606 GN=TRIM25 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 5.7e-05
Identity = 23/49 (46.94%), Postives = 28/49 (57.14%), Query Frame = 0

Query: 170 PAAPSLRCAICIEPLVEETTTKCGHVFCRNCIETAIATQ---HRCPICR 216
           P A  L C+IC+EP  E  TT CGH FC +C+    A Q   + CP CR
Sbjct: 6   PLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCR 54

BLAST of Lag0020965 vs. ExPASy Swiss-Prot
Match: Q61510 (E3 ubiquitin/ISG15 ligase TRIM25 OS=Mus musculus OX=10090 GN=Trim25 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-04
Identity = 21/54 (38.89%), Postives = 31/54 (57.41%), Query Frame = 0

Query: 166 SSIPPAAPSLRCAICIEPLVEETTTKCGHVFCRNCIETAIATQ---HRCPICRR 217
           + + P A  L C++C+E   E  TT CGH FC +C++     Q   +RCP CR+
Sbjct: 2   AELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRK 55

BLAST of Lag0020965 vs. ExPASy TrEMBL
Match: A0A6J1CZ44 (uncharacterized protein LOC111016098 OS=Momordica charantia OX=3673 GN=LOC111016098 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 2.8e-196
Identity = 364/418 (87.08%), Postives = 391/418 (93.54%), Query Frame = 0

Query: 309 MPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLES 368
           MPESMEATPSV P LDLQAVR RISELEEL RSLEEDE+S+TDSLGSEKLLKECA+HLES
Sbjct: 1   MPESMEATPSVPPILDLQAVRCRISELEELQRSLEEDEASSTDSLGSEKLLKECALHLES 60

Query: 369 RLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKL 428
           RLQQ+LSE SNV+SFLGIDDLDAYVE MKEELVMVEAESSKISNEIE +KR N+EDSN+L
Sbjct: 61  RLQQILSEFSNVDSFLGIDDLDAYVEHMKEELVMVEAESSKISNEIEAIKRNNIEDSNRL 120

Query: 429 KMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNK 488
           KMDLEVLK S+D   TS+DPEKATFNC S +GEDQ+NMI +RECNAFEVL+LDSQIE+NK
Sbjct: 121 KMDLEVLKLSLD-RLTSEDPEKATFNCRSPDGEDQINMIDKRECNAFEVLQLDSQIEQNK 180

Query: 489 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLE 548
           RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVA+NFIRLSL THIPNLE  SSLQRLE
Sbjct: 181 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVAENFIRLSLRTHIPNLEDLSSLQRLE 240

Query: 549 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKVQDR 608
           G+IEPSEL+HELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSF NSSLEWFVRKVQDR
Sbjct: 241 GVIEPSELNHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFSNSSLEWFVRKVQDR 300

Query: 609 IVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISL 668
           IVLCTLRR VVK+ANKSSHSFEYLD+D TIICTMIGGI A+I+V QGWPLSDSPLKLISL
Sbjct: 301 IVLCTLRRFVVKTANKSSHSFEYLDEDNTIICTMIGGIGAVIRVSQGWPLSDSPLKLISL 360

Query: 669 KNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSAL 727
           KNSDHYTKG+SLSLICKVEKMANSLD R+R NLS+FADAIEKILKE+MHLEL +D AL
Sbjct: 361 KNSDHYTKGISLSLICKVEKMANSLDVRIRHNLSSFADAIEKILKERMHLELHSDGAL 417

BLAST of Lag0020965 vs. ExPASy TrEMBL
Match: A0A6J1F0V9 (uncharacterized protein LOC111441363 OS=Cucurbita moschata OX=3662 GN=LOC111441363 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 8.9e-195
Identity = 367/418 (87.80%), Postives = 388/418 (92.82%), Query Frame = 0

Query: 309 MPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLES 368
           M E MEATPSVSPS+D+QAVRS ISELEEL RSLEEDE+ TTDSLGS KLLKEC++ LES
Sbjct: 1   MAELMEATPSVSPSVDVQAVRSCISELEELQRSLEEDEAYTTDSLGSGKLLKECSLLLES 60

Query: 369 RLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKL 428
           RLQQ LSE SNV+SFLGIDDLDAYVERMKEEL+ VEAESSKISNEIEVLKRT++EDSNKL
Sbjct: 61  RLQQTLSEWSNVDSFLGIDDLDAYVERMKEELIAVEAESSKISNEIEVLKRTSIEDSNKL 120

Query: 429 KMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNK 488
           +MDLEVLK S+D    SQDPEKAT NC SVNGEDQM+MIVERECNAFEVLELDSQIEKN+
Sbjct: 121 RMDLEVLKLSLD-RCASQDPEKATVNCYSVNGEDQMDMIVERECNAFEVLELDSQIEKNE 180

Query: 489 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLE 548
           R LKSLQELDEIFKSLDVIEQVEDTIGGLKVIDV DNFIRLSLH+HIPNLE FSSLQRLE
Sbjct: 181 RTLKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVTDNFIRLSLHSHIPNLEEFSSLQRLE 240

Query: 549 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKVQDR 608
           GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSF NSSLEWFVRKVQDR
Sbjct: 241 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFSNSSLEWFVRKVQDR 300

Query: 609 IVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISL 668
           IVLC LRR VVKSANKSSHSFEYLDQDETIICTMIGGIDA+IKV QGWPLSDSPLKLISL
Sbjct: 301 IVLCALRRFVVKSANKSSHSFEYLDQDETIICTMIGGIDAVIKVPQGWPLSDSPLKLISL 360

Query: 669 KNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSAL 727
           K+SDHY  GVSLSLICKVEKMANSLD  +R +LS+FADA+EKI+KEQMHLELQ DSAL
Sbjct: 361 KSSDHYKNGVSLSLICKVEKMANSLDVGLRHDLSSFADAVEKIMKEQMHLELQFDSAL 417

BLAST of Lag0020965 vs. ExPASy TrEMBL
Match: A0A6J1IHB4 (uncharacterized protein LOC111473498 OS=Cucurbita maxima OX=3661 GN=LOC111473498 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 2.0e-194
Identity = 365/418 (87.32%), Postives = 388/418 (92.82%), Query Frame = 0

Query: 309 MPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLES 368
           M E MEATPSVS S+DLQAVRS ISE+EEL RSLEEDE+ TTDSLGSEKLLKEC++ LES
Sbjct: 1   MAELMEATPSVSLSVDLQAVRSCISEIEELQRSLEEDEAYTTDSLGSEKLLKECSLLLES 60

Query: 369 RLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKL 428
           RLQQ LSE SNV+S LGIDDLDAYVERMKEEL+ VEAESSKISNEIEVLKRT++EDSNKL
Sbjct: 61  RLQQTLSEWSNVDSLLGIDDLDAYVERMKEELIAVEAESSKISNEIEVLKRTSIEDSNKL 120

Query: 429 KMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNK 488
           KMDLEVLK S+D    SQDPEKATFNC SVNGEDQM+M VERECNAFEVLELDSQIEKN+
Sbjct: 121 KMDLEVLKLSLD-RCASQDPEKATFNCYSVNGEDQMDMTVERECNAFEVLELDSQIEKNE 180

Query: 489 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLE 548
           R LKSLQELDEIFKSLDVIEQVEDTIGGLKVIDV DNF+RLSLH+H+PNLE FSSLQRLE
Sbjct: 181 RTLKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVTDNFVRLSLHSHLPNLEEFSSLQRLE 240

Query: 549 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKVQDR 608
           GMIEPSELDHELLIEVLEGTM+LKNAEIFPGDVHLHDIINASKSF NSSLEWFVRKVQDR
Sbjct: 241 GMIEPSELDHELLIEVLEGTMKLKNAEIFPGDVHLHDIINASKSFSNSSLEWFVRKVQDR 300

Query: 609 IVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISL 668
           IVLC LRR VVKSANKSSHSFEYLDQDETIICTMIGGIDA+IKV QGWPLSDSPLKLISL
Sbjct: 301 IVLCALRRFVVKSANKSSHSFEYLDQDETIICTMIGGIDAVIKVSQGWPLSDSPLKLISL 360

Query: 669 KNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSAL 727
           K+SDHY KGVSLSLICKVEKMANSLD  +R +LS+FADA+EKI+KEQMHLELQ DSAL
Sbjct: 361 KSSDHYIKGVSLSLICKVEKMANSLDVGLRHDLSSFADAVEKIMKEQMHLELQFDSAL 417

BLAST of Lag0020965 vs. ExPASy TrEMBL
Match: A0A6J1E9V8 (uncharacterized protein LOC111432106 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111432106 PE=4 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 1.3e-193
Identity = 361/421 (85.75%), Postives = 391/421 (92.87%), Query Frame = 0

Query: 306 EKTMPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVH 365
           +K MPESMEATPSVS SLDLQAVRSRI ELEEL RSL EDE+ +TDSLGSEKLLKECA+H
Sbjct: 2   KKIMPESMEATPSVSSSLDLQAVRSRIRELEELQRSLAEDEAYSTDSLGSEKLLKECALH 61

Query: 366 LESRLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDS 425
           LESRLQQVLSECSNV+SFLGIDDLDAYVE MKEELV VEAESS+ISNEIEVLKRTN+E S
Sbjct: 62  LESRLQQVLSECSNVDSFLGIDDLDAYVEHMKEELVAVEAESSQISNEIEVLKRTNIEGS 121

Query: 426 NKLKMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIE 485
           NKL+++LE+L  S+D  FTSQDPEK TFN CS+NGEDQMN+IV+RE NAFEVLELDS IE
Sbjct: 122 NKLEVNLELLDVSLD-RFTSQDPEKETFNFCSMNGEDQMNVIVDRERNAFEVLELDSHIE 181

Query: 486 KNKRILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQ 545
           KNKRILKSLQE+DEIFKSLDV+EQVEDTIGGLKVI VADNFIRLSL THIPNLE FSSLQ
Sbjct: 182 KNKRILKSLQEVDEIFKSLDVVEQVEDTIGGLKVIGVADNFIRLSLRTHIPNLEDFSSLQ 241

Query: 546 RLEGMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKV 605
           RLEGMIEPSEL+HELLIEVLEGTMELKNAEIFPGDVHLHDIINASKS  N SLEWFV+KV
Sbjct: 242 RLEGMIEPSELNHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSVSN-SLEWFVKKV 301

Query: 606 QDRIVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKL 665
           QDRIVLCTLRR VVKSANKSSHSF+Y+DQDETI+C MIGGIDA IKV QGWPL+DSPLKL
Sbjct: 302 QDRIVLCTLRRFVVKSANKSSHSFDYIDQDETIVCCMIGGIDAFIKVSQGWPLADSPLKL 361

Query: 666 ISLKNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSA 725
           +SLK+SDHYTKG SLSL+CKVEKMANSLDAR+RQNLS+FADA+EKILKEQMHLEL+AD  
Sbjct: 362 VSLKSSDHYTKGASLSLVCKVEKMANSLDARIRQNLSSFADAVEKILKEQMHLELEADGG 420

Query: 726 L 727
           L
Sbjct: 422 L 420

BLAST of Lag0020965 vs. ExPASy TrEMBL
Match: A0A0A0L6Q3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G129760 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 3.2e-192
Identity = 359/416 (86.30%), Postives = 386/416 (92.79%), Query Frame = 0

Query: 309 MPESMEATPSVSPSLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLES 368
           MPESMEATPSV PSLDLQAVR   SELEEL RSLEE+E STTDSLGSEKLL+ECA+HLES
Sbjct: 2   MPESMEATPSVPPSLDLQAVR---SELEELQRSLEENEESTTDSLGSEKLLRECALHLES 61

Query: 369 RLQQVLSECSNVESFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKL 428
           R+QQVLSE SNV+SFLGIDDLDAYVE MKEELV VEAESSKISNEIEVLKRTN+EDSNKL
Sbjct: 62  RIQQVLSEYSNVDSFLGIDDLDAYVEHMKEELVAVEAESSKISNEIEVLKRTNIEDSNKL 121

Query: 429 KMDLEVLKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNK 488
           KMDLEVLK S+D  F SQDPE+ATFNC S+NGED MN+IV RECNAFEVLEL+SQIEKNK
Sbjct: 122 KMDLEVLKLSLD-RFPSQDPEEATFNCSSMNGEDPMNVIVNRECNAFEVLELESQIEKNK 181

Query: 489 RILKSLQELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLE 548
           +ILKSLQE+DEIFKSLDVIEQVE TIGG+KVIDVADN IRLSLHTHIPN+E FS+LQRLE
Sbjct: 182 KILKSLQEVDEIFKSLDVIEQVEGTIGGMKVIDVADNSIRLSLHTHIPNVEDFSTLQRLE 241

Query: 549 GMIEPSELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFRNSSLEWFVRKVQDR 608
           G+IE SELDHEL+IEVL+GTMELKNAEIFP DVHLHDIINASKS  NSSLEWFVRKVQDR
Sbjct: 242 GLIEKSELDHELIIEVLDGTMELKNAEIFPADVHLHDIINASKSISNSSLEWFVRKVQDR 301

Query: 609 IVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISL 668
           IVLCTLRR  VKSANKS HSFEYLDQDE I+C+MIGGIDA IKV QGWPL+DSPLKLISL
Sbjct: 302 IVLCTLRRFAVKSANKSCHSFEYLDQDEMIMCSMIGGIDACIKVSQGWPLADSPLKLISL 361

Query: 669 KNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADS 725
           K+SDHYTKGVSLSLICKVEKMANSLDA +R+NLS+FADA+EKILKEQMHLELQADS
Sbjct: 362 KSSDHYTKGVSLSLICKVEKMANSLDAHIRRNLSSFADAVEKILKEQMHLELQADS 413

BLAST of Lag0020965 vs. TAIR 10
Match: AT3G23910.1 (BEST Arabidopsis thaliana protein match is: RNA-directed DNA polymerase (reverse transcriptase)-related family protein (TAIR:AT3G24255.2); Has 562 Blast hits to 532 proteins in 147 species: Archae - 28; Bacteria - 51; Metazoa - 157; Fungi - 82; Plants - 85; Viruses - 6; Other Eukaryotes - 153 (source: NCBI BLink). )

HSP 1 Score: 323.6 bits (828), Expect = 4.2e-88
Identity = 184/415 (44.34%), Postives = 274/415 (66.02%), Query Frame = 0

Query: 322 SLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLESRLQQVLSECSNVE 381
           SLDLQ +R R+ EL+   R+  E+   +  S     ++++  +  E ++++++ E  +V+
Sbjct: 9   SLDLQEIRRRVKELDFFPRNCREEPVESCSSDYETLVVQDFVLQFEPKVKEIVEEYGDVD 68

Query: 382 SFLGIDDLDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKLKMDLEVLKFSVDH 441
             L ++D DAY+E ++ EL  VEAES+K+S EIE L +++ +DS++L+ DLE L  S+D 
Sbjct: 69  -LLDVEDSDAYLEYLRNELQSVEAESAKVSEEIERLSQSHAQDSSRLQRDLEGLLLSLD- 128

Query: 442 HFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNKRILKSLQELDEIF 501
             +SQD EK+  N  S +  +   +I   + + F++ EL++Q+E+ + ILKSL++LD + 
Sbjct: 129 SMSSQDVEKSKENQPSSSSMEVCEVI---DDDKFKMFELENQMEEKRMILKSLEDLDSLR 188

Query: 502 KSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLEGMIEPSELDHELL 561
           K  D  EQVED + GLKV++   NFIRL L T+I  L+ F    + + + EPSEL HELL
Sbjct: 189 KRFDAAEQVEDALTGLKVLEFDGNFIRLQLRTYIQKLDGFLGQHKFDHITEPSELIHELL 248

Query: 562 IEVLEGTMELKNAEIFPGDVHLHDIINASKSFR-----------NSSLEWFVRKVQDRIV 621
           I + + T E+   E+FP D+++ DII A+ SFR            SS++W V KVQD+I+
Sbjct: 249 IYLKDKTTEITKFEMFPNDIYIGDIIEAADSFRQVRLHSAVLDTRSSVQWVVAKVQDKII 308

Query: 622 LCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISLKN 681
             TLR+ +V S+    ++FEY D+DETI+  + GGIDA +KV  GWPL ++PLKL SLKN
Sbjct: 309 STTLRKYIVMSSKTIRYTFEYYDKDETIVAHIAGGIDAFLKVSDGWPLLNTPLKLASLKN 368

Query: 682 SDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSA 726
           SD+ +KG+SLSLICKVE++ANSLD   RQNLS F DAIEKIL EQ   ELQ++ +
Sbjct: 369 SDNQSKGISLSLICKVEELANSLDLETRQNLSGFMDAIEKILVEQTREELQSNKS 418

BLAST of Lag0020965 vs. TAIR 10
Match: AT3G24255.2 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 299.3 bits (765), Expect = 8.5e-81
Identity = 177/422 (41.94%), Postives = 268/422 (63.51%), Query Frame = 0

Query: 322 SLDLQAVRSRISELEELHRSLEEDESSTTDSLGSEKLLKECAVHLESRLQQVLSECSNVE 381
           SLDLQ +R R+ E +   R+  E+   +  S     ++++  +  E ++++++ +  +V+
Sbjct: 9   SLDLQEIRRRVKEFDFFPRNCREEPVESCSSDYETLVVQDFVLQFEPKVKEIVEDYGDVD 68

Query: 382 SFLGIDD-------LDAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKLKMDLEV 441
             L +D         DAY+E ++ EL  VEAES+K+S EIE L +++  DS++L+ DLE 
Sbjct: 69  -LLDVDHTLVDGNLTDAYLEYLRNELQSVEAESAKVSEEIERLSQSHALDSSRLQRDLEG 128

Query: 442 LKFSVDHHFTSQDPEKATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNKRILKSL 501
           L  S+D   +SQD EK+  N  S +  +   +I   + + F++ EL++Q+E+ + ILKSL
Sbjct: 129 LLLSLD-SMSSQDVEKSKENQPSSSSMEVCEVI---DDDKFKMFELENQMEEKRMILKSL 188

Query: 502 QELDEIFKSLDVIEQVEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLEGMIEPS 561
           ++LD + K  D  EQVED + GLKV++   NFIRL L T+I  L+ F    + + + EPS
Sbjct: 189 EDLDSLRKRFDAAEQVEDALTGLKVLEFDGNFIRLQLRTYIQKLDGFLGQHKFDHITEPS 248

Query: 562 ELDHELLIEVLEGTMELKNAEIFPGDVHLHDIINASKSFR-----------NSSLEWFVR 621
           EL HELLI + + T E+   E+FP D+++ DII A+ SFR            SS++W V 
Sbjct: 249 ELIHELLIYLKDKTTEITKFEMFPNDIYIGDIIEAADSFRQVRLHSAVLDTRSSVQWVVA 308

Query: 622 KVQDRIVLCTLRRVVVKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPL 681
           KVQD+I+  TLR+  V S+    ++FEY D+DETI+  + GGIDA +KV  GWPL ++PL
Sbjct: 309 KVQDKIISTTLRKDFVMSSKTIRYTFEYYDKDETIVAHIAGGIDAFLKVSDGWPLLNTPL 368

Query: 682 KLISLKNSDHYTKGVSLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQAD 726
           KL SLKNSD+ +KG SLSLI K+E++ANSLD   RQNLS F DA+EKIL +Q   EL+++
Sbjct: 369 KLASLKNSDNQSKGFSLSLISKLEELANSLDLETRQNLSGFMDAVEKILVQQTREELKSN 425

BLAST of Lag0020965 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 285.4 bits (729), Expect = 1.3e-76
Identity = 162/347 (46.69%), Postives = 232/347 (66.86%), Query Frame = 0

Query: 390 DAYVERMKEELVMVEAESSKISNEIEVLKRTNMEDSNKLKMDLEVLKFSVDHHFTSQDPE 449
           DAY+E ++ EL  VEAES+K+S EIE L +++  DS++L+ DLE L  S+D   +SQD E
Sbjct: 401 DAYLEYLRNELQSVEAESAKVSEEIERLSQSHALDSSRLQRDLEGLLLSLD-SMSSQDVE 460

Query: 450 KATFNCCSVNGEDQMNMIVERECNAFEVLELDSQIEKNKRILKSLQELDEIFKSLDVIEQ 509
           K+  N  S +  +   +I   + + F++ EL++Q+E+ + ILKSL++LD + K  D  EQ
Sbjct: 461 KSKENQPSSSSMEVCEVI---DDDKFKMFELENQMEEKRMILKSLEDLDSLRKRFDAAEQ 520

Query: 510 VEDTIGGLKVIDVADNFIRLSLHTHIPNLEYFSSLQRLEGMIEPSELDHELLIEVLEGTM 569
           VED + GLKV++   NFIRL L T+I  L+ F    + + + EPSEL HELLI + + T 
Sbjct: 521 VEDALTGLKVLEFDGNFIRLQLRTYIQKLDGFLGQHKFDHITEPSELIHELLIYLKDKTT 580

Query: 570 ELKNAEIFPGDVHLHDIINASKSFR-----------NSSLEWFVRKVQDRIVLCTLRRVV 629
           E+   E+FP D+++ DII A+ SFR            SS++W V KVQD+I+  TLR+  
Sbjct: 581 EITKFEMFPNDIYIGDIIEAADSFRQVRLHSAVLDTRSSVQWVVAKVQDKIISTTLRKDF 640

Query: 630 VKSANKSSHSFEYLDQDETIICTMIGGIDALIKVFQGWPLSDSPLKLISLKNSDHYTKGV 689
           V S+    ++FEY D+DETI+  + GGIDA +KV  GWPL ++PLKL SLKNSD+ +KG 
Sbjct: 641 VMSSKTIRYTFEYYDKDETIVAHIAGGIDAFLKVSDGWPLLNTPLKLASLKNSDNQSKGF 700

Query: 690 SLSLICKVEKMANSLDARVRQNLSTFADAIEKILKEQMHLELQADSA 726
           SLSLI K+E++ANSLD   RQNLS F DA+EKIL +Q   EL+++ +
Sbjct: 701 SLSLISKLEELANSLDLETRQNLSGFMDAVEKILVQQTREELKSNES 743

BLAST of Lag0020965 vs. TAIR 10
Match: AT5G48655.2 (RING/U-box superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 9.9e-13
Identity = 56/200 (28.00%), Postives = 86/200 (43.00%), Query Frame = 0

Query: 19  RRTVMDFDLNCPPPDECIDPTVPHEEAAQYYNRYPGQAMDNPEFVDEDIAIISPRKFAEA 78
           RR     DLN  P D+                  P   M + + +++D+   S   FAEA
Sbjct: 13  RRRKAVIDLNAVPVDQEGTSASVRTLTVPITPSQPAPTMIDVDAIEDDVIESSASAFAEA 72

Query: 79  RKNFRRNHFESSCGVAVRRNGNTEVYGSLSDVTSWPPFTIWPPLTISNNVSIQEQAIHNL 138
            K+  RN       V V   G T                   P  ISN    + + I + 
Sbjct: 73  -KSKSRNARRRPLMVDVESGGTTRF-----------------PANISN----KRRRIPSS 132

Query: 139 DLCLSCESSSRAIKATDTDIPSALAQNS--SIPPAAPSLRCAICIEPLVEETTTKCGHVF 198
           +  + CE +S      + ++ S ++++   + PP  P   C IC+ P  EE +TKCGH+F
Sbjct: 133 ESVIDCEHAS---VNDEVNMSSRVSRSKAPAPPPEEPKFTCPICMCPFTEEMSTKCGHIF 187

Query: 199 CRNCIETAIATQHRCPICRR 217
           C+ CI+ AI+ Q +CP CR+
Sbjct: 193 CKGCIKMAISRQGKCPTCRK 187

BLAST of Lag0020965 vs. TAIR 10
Match: AT5G48655.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 9.9e-13
Identity = 56/200 (28.00%), Postives = 86/200 (43.00%), Query Frame = 0

Query: 19  RRTVMDFDLNCPPPDECIDPTVPHEEAAQYYNRYPGQAMDNPEFVDEDIAIISPRKFAEA 78
           RR     DLN  P D+                  P   M + + +++D+   S   FAEA
Sbjct: 13  RRRKAVIDLNAVPVDQEGTSASVRTLTVPITPSQPAPTMIDVDAIEDDVIESSASAFAEA 72

Query: 79  RKNFRRNHFESSCGVAVRRNGNTEVYGSLSDVTSWPPFTIWPPLTISNNVSIQEQAIHNL 138
            K+  RN       V V   G T                   P  ISN    + + I + 
Sbjct: 73  -KSKSRNARRRPLMVDVESGGTTRF-----------------PANISN----KRRRIPSS 132

Query: 139 DLCLSCESSSRAIKATDTDIPSALAQNS--SIPPAAPSLRCAICIEPLVEETTTKCGHVF 198
           +  + CE +S      + ++ S ++++   + PP  P   C IC+ P  EE +TKCGH+F
Sbjct: 133 ESVIDCEHAS---VNDEVNMSSRVSRSKAPAPPPEEPKFTCPICMCPFTEEMSTKCGHIF 187

Query: 199 CRNCIETAIATQHRCPICRR 217
           C+ CI+ AI+ Q +CP CR+
Sbjct: 193 CKGCIKMAISRQGKCPTCRK 187