Lag0020931 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020931
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein kinase domain-containing protein
Locationchr7: 3231704 .. 3233554 (-)
RNA-Seq ExpressionLag0020931
SyntenyLag0020931
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTGCTTCTTCTACTTCAGAGACAAGACCAGGAGTAAGCATCAAAGATCGGCTCCTGAATTGAAAGAGCAGCAAGAATCTGACTTTTCGGGGTGTAGTCGGACCGGTGCTTCTTCGTGCTCTCTGGCTTCTTCTCGGAGTGTACCTGAATTGTATGAAGAGAAAGCACATAATTTGAGAGTTTTCTCATTTGCAGAGCTGAGACAGGCAACGCATGACTTTGACAGGCTTCTTAAGATTGGGCAAGGGGGATTTGGGAGTGTCTTTAAAGGCTCAATTAAGCCTGTTGATGGGAAGGGAGATCCCCTTGTTGTTGCTATCAAGCAACTGAGCAAGGATGGCTTACAGGTATGGATGTTTACCTTAGAAAATAAGTCCTAAGGGTTCTTTTTTAATGTTTTGCTTTGAATTTAATGGGTGATTTTCTTCTTTCTGGGAAGATGCTGATTGGGTGATTTGGTTATATTCAGTCCATATTATATGTATGCATCCAGTACAGAATCAATACTATTATTTGATAAGTTTTAATCCTGTGGAAAAGAATGATGAGCAAGAAAACAGGGAAAGGGCAAATAAGTGCATTTTTCAAGCTGTTTCCTTTGGATGTGATGCAAATTTCTTGAGGAAGTGATTTTGTTAATACATACCATCTGTATTTTGCAAATAGGGTCACAAGCAGTGGCTGGCAGAAGTTCAATTTCTTGGTATTGTGGAGCATCCAAATCTTGTCAAACTCATAGGATACTGTGCTGTGGATGGAGCAAGAGGAATTCAAAGACTGCTTGTATATGAGTATATGACGAACAGAAGCTTGGAAGATCATCTCTTCAACAAGGCGCTACCAACACTCGCTTGGAAAACAAGATTGCAGATAGTACTTGGAGCAGCTCAAGGATTGGCTTATCTGCATGAAGGATTGGAAGTTCAGGTACCATATCCGCTAAAATTGAATTCAATGCGTGAGTGTGTGCATGTATATGTCTGCAAACAAGAAAGTTATAACAAGCATACCATCTTTTGCTGAAGTTTTTCGTTCTTCAGAACTTCCATAATGATTAGATTTACTTTAGTTTTCTTCCATAATGGCACAGATAATATACAGAGATTTCAAGAGCTCAAATGTGTTACTGGATGAGAATTTTCATCCAAAACTTTCAGATTTTGGGCTTGCCAGGGAAGGACCAGAAATTGGGCGCACTCATGTTTCAACAGCAGTAAGTTTTCTTTGTGTTCTTCCCCTTTAATCACTCTCAGGCTGCATTGATGCTTACAGTGAACTATATCACGTACACTCAGGTAATGGGGACTAATGGATATGCAGCTCCAGACTACATCAAGACAGGCCATCTCACAGCAAAAAGCGATGTATGGAGTTTTGGTGTGGTGCTTTATGAGATTCTGACAGGAAGACGATCATTGGATAGACATCGTCCGAGACCAGAACAGAAACTTGTGGAATGGGTTAAATATTTCAACCCTGACAGCAAAAGGTTTAGTTTGATAATAGATCCTCGGCTTGAAAATCAGTATCCTATCAATGCAGCAAGAGAAGTAGCAAAGTTAGCAGATACTTGCCTCGCAAAGAGTGCAAAAGATAGGCCCTCAATGGCAGAGGTAGTAAACAGATTGAAGCAGATCATCAAAGACAAGGACGACGAAGCACCTTACAAGAAAAGTCCTGATTCCATCGATGACGAACCTGAAATGGAAAGAGAGCCAGAGAAAATGGAGGCACAGGCACCGGAGTCATGGAGAAGGCGGATGAGCCACCTGCAGAAATTGGGAGAGCATGTGGAGGGTGCAAGTAGAAGAAGATTTATGATAATGCAAAGAGCAAAAGTGCCTTAA

mRNA sequence

ATGAAGTGCTTCTTCTACTTCAGAGACAAGACCAGGAGTAAGCATCAAAGATCGGCTCCTGAATTGAAAGAGCAGCAAGAATCTGACTTTTCGGGGTGTAGTCGGACCGGTGCTTCTTCGTGCTCTCTGGCTTCTTCTCGGAGTGTACCTGAATTGTATGAAGAGAAAGCACATAATTTGAGAGTTTTCTCATTTGCAGAGCTGAGACAGGCAACGCATGACTTTGACAGGCTTCTTAAGATTGGGCAAGGGGGATTTGGGAGTGTCTTTAAAGGCTCAATTAAGCCTGTTGATGGGAAGGGAGATCCCCTTGTTGTTGCTATCAAGCAACTGAGCAAGGATGGCTTACAGGGTCACAAGCAGTGGCTGGCAGAAGTTCAATTTCTTGGTATTGTGGAGCATCCAAATCTTGTCAAACTCATAGGATACTGTGCTGTGGATGGAGCAAGAGGAATTCAAAGACTGCTTGTATATGAGTATATGACGAACAGAAGCTTGGAAGATCATCTCTTCAACAAGGCGCTACCAACACTCGCTTGGAAAACAAGATTGCAGATAGTACTTGGAGCAGCTCAAGGATTGGCTTATCTGCATGAAGGATTGGAAGTTCAGATAATATACAGAGATTTCAAGAGCTCAAATGTGTTACTGGATGAGAATTTTCATCCAAAACTTTCAGATTTTGGGCTTGCCAGGGAAGGACCAGAAATTGGGCGCACTCATGTTTCAACAGCAGTAATGGGGACTAATGGATATGCAGCTCCAGACTACATCAAGACAGGCCATCTCACAGCAAAAAGCGATGTATGGAGTTTTGGTGTGGTGCTTTATGAGATTCTGACAGGAAGACGATCATTGGATAGACATCGTCCGAGACCAGAACAGAAACTTGTGGAATGGGTTAAATATTTCAACCCTGACAGCAAAAGGTTTAGTTTGATAATAGATCCTCGGCTTGAAAATCAGTATCCTATCAATGCAGCAAGAGAAGTAGCAAAGTTAGCAGATACTTGCCTCGCAAAGAGTGCAAAAGATAGGCCCTCAATGGCAGAGGTAGTAAACAGATTGAAGCAGATCATCAAAGACAAGGACGACGAAGCACCTTACAAGAAAAGTCCTGATTCCATCGATGACGAACCTGAAATGGAAAGAGAGCCAGAGAAAATGGAGGCACAGGCACCGGAGTCATGGAGAAGGCGGATGAGCCACCTGCAGAAATTGGGAGAGCATGTGGAGGGTGCAAGTAGAAGAAGATTTATGATAATGCAAAGAGCAAAAGTGCCTTAA

Coding sequence (CDS)

ATGAAGTGCTTCTTCTACTTCAGAGACAAGACCAGGAGTAAGCATCAAAGATCGGCTCCTGAATTGAAAGAGCAGCAAGAATCTGACTTTTCGGGGTGTAGTCGGACCGGTGCTTCTTCGTGCTCTCTGGCTTCTTCTCGGAGTGTACCTGAATTGTATGAAGAGAAAGCACATAATTTGAGAGTTTTCTCATTTGCAGAGCTGAGACAGGCAACGCATGACTTTGACAGGCTTCTTAAGATTGGGCAAGGGGGATTTGGGAGTGTCTTTAAAGGCTCAATTAAGCCTGTTGATGGGAAGGGAGATCCCCTTGTTGTTGCTATCAAGCAACTGAGCAAGGATGGCTTACAGGGTCACAAGCAGTGGCTGGCAGAAGTTCAATTTCTTGGTATTGTGGAGCATCCAAATCTTGTCAAACTCATAGGATACTGTGCTGTGGATGGAGCAAGAGGAATTCAAAGACTGCTTGTATATGAGTATATGACGAACAGAAGCTTGGAAGATCATCTCTTCAACAAGGCGCTACCAACACTCGCTTGGAAAACAAGATTGCAGATAGTACTTGGAGCAGCTCAAGGATTGGCTTATCTGCATGAAGGATTGGAAGTTCAGATAATATACAGAGATTTCAAGAGCTCAAATGTGTTACTGGATGAGAATTTTCATCCAAAACTTTCAGATTTTGGGCTTGCCAGGGAAGGACCAGAAATTGGGCGCACTCATGTTTCAACAGCAGTAATGGGGACTAATGGATATGCAGCTCCAGACTACATCAAGACAGGCCATCTCACAGCAAAAAGCGATGTATGGAGTTTTGGTGTGGTGCTTTATGAGATTCTGACAGGAAGACGATCATTGGATAGACATCGTCCGAGACCAGAACAGAAACTTGTGGAATGGGTTAAATATTTCAACCCTGACAGCAAAAGGTTTAGTTTGATAATAGATCCTCGGCTTGAAAATCAGTATCCTATCAATGCAGCAAGAGAAGTAGCAAAGTTAGCAGATACTTGCCTCGCAAAGAGTGCAAAAGATAGGCCCTCAATGGCAGAGGTAGTAAACAGATTGAAGCAGATCATCAAAGACAAGGACGACGAAGCACCTTACAAGAAAAGTCCTGATTCCATCGATGACGAACCTGAAATGGAAAGAGAGCCAGAGAAAATGGAGGCACAGGCACCGGAGTCATGGAGAAGGCGGATGAGCCACCTGCAGAAATTGGGAGAGCATGTGGAGGGTGCAAGTAGAAGAAGATTTATGATAATGCAAAGAGCAAAAGTGCCTTAA

Protein sequence

MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQIIKDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP
Homology
BLAST of Lag0020931 vs. NCBI nr
Match: KAG6582509.1 (putative serine/threonine-protein kinase PBL19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018894.1 putative serine/threonine-protein kinase PBL19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 778.9 bits (2010), Expect = 2.3e-221
Identity = 392/428 (91.59%), Postives = 404/428 (94.39%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYFR+K RS+HQRSAPELKEQQESD SGCSRT AS  SL S RSVPELYEEKAHNL
Sbjct: 1   MKCFFYFRNKNRSRHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQATHDF+RLLKIGQGGFGSVFKGSIKPVDGKG+PLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFL IVEHPNLVKLIGYCAVDG RGIQRLLVYEYM NRSLEDHLFNKALP LAW
Sbjct: 121 QWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFNKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           KTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT
Sbjct: 181 KTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSL+RHR RPEQKLVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRPEQKLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDS RFSLIIDPRLENQYPINAAR+VAKLADTCLAK+AKDRPSMAEVVNRLK II
Sbjct: 301 VKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +DD  PY KSPDS++DEP MEREPEKME  AP SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KAEDDSEPYAKSPDSVNDEPGMEREPEKME--APMSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. NCBI nr
Match: XP_022924530.1 (probable serine/threonine-protein kinase PBL19 [Cucurbita moschata] >XP_022924531.1 probable serine/threonine-protein kinase PBL19 [Cucurbita moschata])

HSP 1 Score: 778.1 bits (2008), Expect = 3.9e-221
Identity = 391/428 (91.36%), Postives = 404/428 (94.39%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYFR+K RS+HQRSAPELKEQQESD SGCSRT AS  SL S RSVPELYEEKAHNL
Sbjct: 1   MKCFFYFRNKNRSRHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQATHDF+RLLKIGQGGFGSVFKGSIKPVDGKG+PLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFL IVEHPNLVKLIGYCAVDG RGIQRLLVYEYM NRSLEDHLF KALP LAW
Sbjct: 121 QWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFKKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           KTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT
Sbjct: 181 KTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSL+RHR RPEQKLVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRPEQKLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDS RFSLIIDPRLENQYPINAAR+VAKLADTCLAK+AKDRPSMAEVVNRLK II
Sbjct: 301 VKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +DD  PY KSPDS++DEP+MEREPEKME  AP SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KAEDDSEPYAKSPDSVNDEPDMEREPEKME--APMSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. NCBI nr
Match: XP_038892560.1 (probable serine/threonine-protein kinase PBL19 [Benincasa hispida])

HSP 1 Score: 774.2 bits (1998), Expect = 5.7e-220
Identity = 385/428 (89.95%), Postives = 409/428 (95.56%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYF+ K+RS+HQRSAPELKEQQES+FSGCSRT ASSCS+ S RSVPELYEE+AHNL
Sbjct: 1   MKCFFYFKGKSRSRHQRSAPELKEQQESEFSGCSRTAASSCSVTSPRSVPELYEERAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELR+ATHDF+RLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRRATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFLGIVEHPNLVKLIGYCAVDG+R IQRLLVYEYM NRSLEDHLFNKALP LAW
Sbjct: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGSRAIQRLLVYEYMPNRSLEDHLFNKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPE+GRT
Sbjct: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEVGRT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYI+TGHLTAKSDVWS GVVLYEILTGRRSL+R+R RPEQKLVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIETGHLTAKSDVWSLGVVLYEILTGRRSLERNRSRPEQKLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDSK+F LIIDPRLENQYPINAAR++AKLADTCLAK+AKDRPSMAEVVNRLKQII
Sbjct: 301 VKHFNPDSKKFRLIIDPRLENQYPINAARKLAKLADTCLAKNAKDRPSMAEVVNRLKQII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
             +D   PY++SPDS+ DEP+MEREPEK+E  APESWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 IAEDGSEPYEQSPDSVTDEPDMEREPEKVE--APESWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. NCBI nr
Match: XP_023527511.1 (probable serine/threonine-protein kinase PBL19 [Cucurbita pepo subsp. pepo] >XP_023527512.1 probable serine/threonine-protein kinase PBL19 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.9 bits (1997), Expect = 7.4e-220
Identity = 389/428 (90.89%), Postives = 403/428 (94.16%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYFR+K RS+HQ SAPELKEQQESDFSGCSRT AS  SL S RSVPELYEEKAHNL
Sbjct: 1   MKCFFYFRNKNRSRHQTSAPELKEQQESDFSGCSRTAASLSSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQATHDF+RLLKIGQGGFGSVFKGSIKPVDGKG+PLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFL IVEHPNLVKLIGYCAVDG RGIQRLLVYEYM NRSLEDHLFNKALP LAW
Sbjct: 121 QWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFNKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           +TRLQIVLGAAQGLAYLHEGLE+QIIYRD KSSNVLLDENFHPKLSDFGLAREGPEIGRT
Sbjct: 181 RTRLQIVLGAAQGLAYLHEGLEIQIIYRDLKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSL+RHR RPEQKLVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRPEQKLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDS RFSLIIDPRLENQYPINAAR+VAKLADTCLAK+AKDRPSMAEVVNRLK II
Sbjct: 301 VKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +DD  PY KSPDS++DE +MEREPEKME  AP SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KAEDDNEPYAKSPDSVNDESDMEREPEKME--APMSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. NCBI nr
Match: XP_022980299.1 (probable serine/threonine-protein kinase PBL19 [Cucurbita maxima])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 388/428 (90.65%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYFR+K RS+HQRSAPELKEQQESD SGCSRT AS  SL S RSVPELYEEKAHNL
Sbjct: 1   MKCFFYFRNKNRSRHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQATHDF+RLLKIGQGGFGSVFKGSIKPVDGKG+PLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFL IVEHPNLVKLIGYCAVDG RGIQRLLVYEYM NRSLEDHLFNKALP LAW
Sbjct: 121 QWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFNKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           KTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIG T
Sbjct: 181 KTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGHT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSL+RHR R EQ+LVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRLEQRLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDS RFSLIIDPRLENQYPINAAR+VAKLADTCLAK+AKDRPSMAEVVNRLK II
Sbjct: 301 VKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +D   PY KSPDS++DEP+MEREPEKME  AP SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KAEDVSEPYAKSPDSVNDEPDMEREPEKME--APMSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. ExPASy Swiss-Prot
Match: Q9LTC0 (Probable serine/threonine-protein kinase PBL19 OS=Arabidopsis thaliana OX=3702 GN=PBL19 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.7e-118
Identity = 224/404 (55.45%), Postives = 293/404 (72.52%), Query Frame = 0

Query: 1   MKCFFYFR-------DKTRSKHQRSAPELKEQQESDFSGCSRTGASSC----SLASSRSV 60
           M C F F+        K  +K++R   EL +    + +  S T + +     SL S RS+
Sbjct: 1   MNCLFLFKSKKPRKQQKDNNKNKRKGKELLQNSAPELTNRSETSSFNLQTPRSLPSPRSI 60

Query: 61  PELYEEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSI-KPVDGKGDPLVVAI 120
            +LY E+  NLRVFS+ EL +AT+ F R L IG+GGFG V+KG I    D    PLVVAI
Sbjct: 61  KDLYTEREQNLRVFSYEELSKATYVFSRKLVIGEGGFGIVYKGKILSNGDSSDPPLVVAI 120

Query: 121 KQLSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLED 180
           K+L++ GLQGHKQWLAEVQFLG+V HPN+VKLIGYC+ DG  GI+RLLVYEYM+NRSLED
Sbjct: 121 KKLNRQGLQGHKQWLAEVQFLGVVNHPNVVKLIGYCSEDGETGIERLLVYEYMSNRSLED 180

Query: 181 HLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDF 240
           HLF +   TL WK RL+I+LGAA+GL YLH   ++++IYRDFKSSNVLLD+ F PKLSDF
Sbjct: 181 HLFPRRSHTLPWKKRLEIMLGAAEGLTYLH---DLKVIYRDFKSSNVLLDDQFCPKLSDF 240

Query: 241 GLAREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDR 300
           GLAREGP+   THV+TA +GT+GYAAP+Y++TGHL  KSDV+SFGVVLYEI+TGRR+++R
Sbjct: 241 GLAREGPDGDNTHVTTARVGTHGYAAPEYVQTGHLRLKSDVYSFGVVLYEIITGRRTIER 300

Query: 301 HRPRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPS 360
           ++P  E++L++WVK +  DS+RFS+I+DPRL N YP   AR +AKLAD CL K+ K+RP+
Sbjct: 301 NKPVAERRLLDWVKEYPADSQRFSMIVDPRLRNNYPAAGARSLAKLADLCLKKNDKERPT 360

Query: 361 MAEVVNRLKQIIKDKDDEAPYKKSPDSIDDEPEMER----EPEK 389
           M  VV RLK+II++ D E  Y  +  +  +  ++ R    +PEK
Sbjct: 361 MEIVVERLKKIIEESDSE-DYPMATTTTKESSQVRRRQVAKPEK 400

BLAST of Lag0020931 vs. ExPASy Swiss-Prot
Match: F4JPX3 (Probable serine/threonine-protein kinase PBL20 OS=Arabidopsis thaliana OX=3702 GN=PBL20 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.0e-108
Identity = 209/358 (58.38%), Postives = 262/358 (73.18%), Query Frame = 0

Query: 16  QRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKA----HNLRVFSFAELRQA 75
           Q+SAPEL  ++ +     S    +  SL S  S+ +LY ++      NLRVFSF EL  A
Sbjct: 32  QKSAPELTTRKTT----LSFNLPTPRSLPSPTSIKDLYTDREQNQNQNLRVFSFKELSDA 91

Query: 76  THDFDRLLKIGQGGFGSVFKGSI-KPV--DGKGDPLVVAIKQLSKDGLQGHKQWLAEVQF 135
           T +F R LKIG+GGFGSV+K +I  P   D    PL VA+K+L++  LQGHKQWLAEV F
Sbjct: 92  TCEFSRKLKIGEGGFGSVYKATINNPTVGDSHSSPLTVAVKKLNRQSLQGHKQWLAEVHF 151

Query: 136 LGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAWKTRLQIVL 195
           LG+V HPN+V+L+GYC+ D     +RLLVYE M+NRSLEDHLF     TL+WK RL+I+L
Sbjct: 152 LGVVNHPNVVRLLGYCSEDR----ERLLVYELMSNRSLEDHLFTLRTLTLSWKQRLEIML 211

Query: 196 GAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRTHVSTAVMG 255
           GAAQGLAYLH   E+Q+IYRDFKSSNVLL+E FHPKLSDFGLAREGPE   THV+TA +G
Sbjct: 212 GAAQGLAYLH---EIQVIYRDFKSSNVLLNEEFHPKLSDFGLAREGPEGDNTHVTTARVG 271

Query: 256 TNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEWVKYFNPDS 315
           T+GYAAP+Y+ TGHL    DV+SFGVVLYEI+TGRR+L+R +P  EQKL+EWVK +  +S
Sbjct: 272 TDGYAAPEYVITGHLKTHCDVYSFGVVLYEIITGRRTLERMKPLAEQKLLEWVKKYPINS 331

Query: 316 KRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQIIKDKDDE 367
           KRF +I+D +L N+YPI   R VAKLAD C+ K  K+RP+MA VV  L  II++ + E
Sbjct: 332 KRFKMIVDSKLCNKYPIAMVRRVAKLADHCVNKIDKERPTMAFVVESLTNIIEESNSE 378

BLAST of Lag0020931 vs. ExPASy Swiss-Prot
Match: Q9SF86 (Serine/threonine-protein kinase PCRK1 OS=Arabidopsis thaliana OX=3702 GN=PCRK1 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.4e-104
Identity = 192/371 (51.75%), Postives = 263/371 (70.89%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQR----SAPELKEQQE-----SDFSGCSRTGASS-CSLASSRSVP 60
           MKCF +     R + +     S   +   +E     S+F+    +G S+  S+    S P
Sbjct: 1   MKCFLFSGGDKRGEQKTPISVSLTSIFSDREINRSGSEFNSRDVSGTSTESSMGRKNSYP 60

Query: 61  ELYEEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQ 120
            +   +A NLR FS  +L+ AT +F R + IG+GGFG VF+G+++ ++     + VA+KQ
Sbjct: 61  PV-STRASNLREFSITDLKSATKNFSRSVMIGEGGFGCVFRGTVRNLEDSSVKIEVAVKQ 120

Query: 121 LSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHL 180
           L K GLQGHK+W+ EV FLGIVEH NLVKL+GYCA D  RGIQRLLVYEYM NRS+E HL
Sbjct: 121 LGKRGLQGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHL 180

Query: 181 FNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGL 240
             ++L  L W  RL+I   AA+GL YLHE +E QII+RDFKSSN+LLDE++  KLSDFGL
Sbjct: 181 SPRSLTVLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGL 240

Query: 241 AREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHR 300
           AR GP  G THVST V+GT GYAAP+YI+TG LT+KSDVW +GV LYE++TGRR +DR+R
Sbjct: 241 ARLGPSEGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRRPVDRNR 300

Query: 301 PRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMA 360
           P+ EQKL+EWV+ +  D+++F LI+DPRLE +YPI + +++A +A+ CL +++K RP M+
Sbjct: 301 PKGEQKLLEWVRPYLSDTRKFKLILDPRLEGKYPIKSVQKLAVVANRCLVRNSKARPKMS 360

Query: 361 EVVNRLKQIIK 362
           EV+  + +I++
Sbjct: 361 EVLEMVNKIVE 370

BLAST of Lag0020931 vs. ExPASy Swiss-Prot
Match: Q9LZF8 (Serine/threonine-protein kinase PCRK2 OS=Arabidopsis thaliana OX=3702 GN=PCRK2 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 3.2e-101
Identity = 189/368 (51.36%), Postives = 259/368 (70.38%), Query Frame = 0

Query: 1   MKCFFY----FRDKTRSKHQRSAPEL---KEQQESDFSGCSRTGASSCSLASSRSVPELY 60
           MKCF +     +D+ RS    S         +  SDFS    +G S+ S ++ R+     
Sbjct: 1   MKCFLFPLGDKKDEQRSPKPVSPTSNFSDVNKSGSDFSPRDVSGTSTVS-STGRNSNTSM 60

Query: 61  EEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSK 120
             + +NLR F+  +L+ AT +F R   IG+GGFG VF G+IK ++     + VA+KQL K
Sbjct: 61  SARENNLREFTIGDLKSATRNFSRSGMIGEGGFGCVFWGTIKNLEDPSKKIEVAVKQLGK 120

Query: 121 DGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNK 180
            GLQGHK+W+ EV FLG+VEH NLVKL+G+CA D  RGIQRLLVYEYM N+S+E HL  +
Sbjct: 121 RGLQGHKEWVTEVNFLGVVEHSNLVKLLGHCAEDDERGIQRLLVYEYMPNQSVEFHLSPR 180

Query: 181 ALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLARE 240
           +   L W  RL+I   AA+GL YLHE ++ QII+RDFKSSN+LLDEN+  KLSDFGLAR 
Sbjct: 181 SPTVLTWDLRLRIAQDAARGLTYLHEEMDFQIIFRDFKSSNILLDENWTAKLSDFGLARL 240

Query: 241 GPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRP 300
           GP  G +HVST V+GT GYAAP+YI+TG LT+KSDVW +GV +YE++TGRR LDR++P+ 
Sbjct: 241 GPSPGSSHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFIYELITGRRPLDRNKPKG 300

Query: 301 EQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVV 360
           EQKL+EWV+ +  D++RF LI+DPRLE +Y I + +++A +A+ CL ++AK RP M+EV+
Sbjct: 301 EQKLLEWVRPYLSDTRRFRLIVDPRLEGKYMIKSVQKLAVVANLCLTRNAKARPKMSEVL 360

Query: 361 NRLKQIIK 362
             + +I++
Sbjct: 361 EMVTKIVE 367

BLAST of Lag0020931 vs. ExPASy Swiss-Prot
Match: Q9LFP7 (Serine/threonine-protein kinase PBL34 OS=Arabidopsis thaliana OX=3702 GN=PBL34 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.0e-91
Identity = 176/327 (53.82%), Postives = 233/327 (71.25%), Query Frame = 0

Query: 45  SSRSVPELYEEK--AHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIK-----PV 104
           SS S P + EE   + +LR F+F +L+ +T +F     +G+GGFG VFKG I+     PV
Sbjct: 110 SSSSTPVISEELNISSHLRKFTFNDLKLSTRNFRPESLLGEGGFGCVFKGWIEENGTAPV 169

Query: 105 DGKGDPLVVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLV 164
              G  L VA+K L+ DGLQGHK+WLAE+ FLG + HPNLVKL+GYC  D     QRLLV
Sbjct: 170 K-PGTGLTVAVKTLNPDGLQGHKEWLAEINFLGNLLHPNLVKLVGYCIEDD----QRLLV 229

Query: 165 YEYMTNRSLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLL 224
           YE+M   SLE+HLF ++LP L W  R++I LGAA+GL++LHE     +IYRDFK+SN+LL
Sbjct: 230 YEFMPRGSLENHLFRRSLP-LPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILL 289

Query: 225 DENFHPKLSDFGLAREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLY 284
           D +++ KLSDFGLA++ P+ G+THVST VMGT GYAAP+Y+ TGHLT+KSDV+SFGVVL 
Sbjct: 290 DADYNAKLSDFGLAKDAPDEGKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLL 349

Query: 285 EILTGRRSLDRHRPRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADT 344
           E+LTGRRS+D++RP  E  LVEW +    D +RF  ++DPRLE  + I  A++V +LA  
Sbjct: 350 EMLTGRRSMDKNRPNGEHNLVEWARPHLLDKRRFYRLLDPRLEGHFSIKGAQKVTQLAAQ 409

Query: 345 CLAKSAKDRPSMAEVVNRLKQIIKDKD 365
           CL++  K RP M++VV  LK +   KD
Sbjct: 410 CLSRDPKIRPKMSDVVEALKPLPHLKD 430

BLAST of Lag0020931 vs. ExPASy TrEMBL
Match: A0A6J1EFA4 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=LOC111431988 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.9e-221
Identity = 391/428 (91.36%), Postives = 404/428 (94.39%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYFR+K RS+HQRSAPELKEQQESD SGCSRT AS  SL S RSVPELYEEKAHNL
Sbjct: 1   MKCFFYFRNKNRSRHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQATHDF+RLLKIGQGGFGSVFKGSIKPVDGKG+PLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFL IVEHPNLVKLIGYCAVDG RGIQRLLVYEYM NRSLEDHLF KALP LAW
Sbjct: 121 QWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFKKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           KTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT
Sbjct: 181 KTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSL+RHR RPEQKLVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRPEQKLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDS RFSLIIDPRLENQYPINAAR+VAKLADTCLAK+AKDRPSMAEVVNRLK II
Sbjct: 301 VKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +DD  PY KSPDS++DEP+MEREPEKME  AP SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KAEDDSEPYAKSPDSVNDEPDMEREPEKME--APMSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. ExPASy TrEMBL
Match: A0A6J1IYW5 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita maxima OX=3661 GN=LOC111479715 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.2e-219
Identity = 388/428 (90.65%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCFFYFR+K RS+HQRSAPELKEQQESD SGCSRT AS  SL S RSVPELYEEKAHNL
Sbjct: 1   MKCFFYFRNKNRSRHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQATHDF+RLLKIGQGGFGSVFKGSIKPVDGKG+PLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFL IVEHPNLVKLIGYCAVDG RGIQRLLVYEYM NRSLEDHLFNKALP LAW
Sbjct: 121 QWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFNKALPALAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           KTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIG T
Sbjct: 181 KTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGHT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSL+RHR R EQ+LVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRLEQRLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDS RFSLIIDPRLENQYPINAAR+VAKLADTCLAK+AKDRPSMAEVVNRLK II
Sbjct: 301 VKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +D   PY KSPDS++DEP+MEREPEKME  AP SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KAEDVSEPYAKSPDSVNDEPDMEREPEKME--APMSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQRAKVP
Sbjct: 421 IMQRAKVP 426

BLAST of Lag0020931 vs. ExPASy TrEMBL
Match: A0A6J1IB57 (probable serine/threonine-protein kinase PBL19 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111473487 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 5.7e-218
Identity = 379/429 (88.34%), Postives = 408/429 (95.10%), Query Frame = 0

Query: 1   MKCFFYFRDKTRS-KHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHN 60
           MKCFFYFR+K+RS +HQRSAPELK + ESDFSGCSRT ASSCSL S+RSVPELYEEKAHN
Sbjct: 1   MKCFFYFRNKSRSRRHQRSAPELKGELESDFSGCSRTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAE+R+ATHDF+RLLKIGQGGFGSVFKGSIKP+DGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLV+L+GYCAVDG RGIQRLL+YEY+TNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLIYEYVTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPE GR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS+GVVLYEILTGRRSL+RH PRPEQ LVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQI 360
           WVKY NPDSK+FS IIDPRLEN+YPIN AR+VAKLA+TCL K+AKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSKKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRF 420
           IK +DD+ PY+KSPD+ D++P+MEREPEK +    +SWRRRMSHLQKLGEHVEGASRRRF
Sbjct: 361 IKAEDDDEPYEKSPDNSDNKPDMEREPEKTKVM--DSWRRRMSHLQKLGEHVEGASRRRF 420

Query: 421 MIMQRAKVP 429
           MIMQRAKVP
Sbjct: 421 MIMQRAKVP 427

BLAST of Lag0020931 vs. ExPASy TrEMBL
Match: A0A0A0L4J8 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128960 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 5.7e-218
Identity = 383/428 (89.49%), Postives = 406/428 (94.86%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCF+YF+DK+RS+ QRSAP+LKEQQESDFSGCSRT ASSCSL S RSVPELYEEKAHNL
Sbjct: 1   MKCFYYFKDKSRSRRQRSAPQLKEQQESDFSGCSRTAASSCSLTSPRSVPELYEEKAHNL 60

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           RVFSF ELRQAT DF+RLLKIGQGGFGSVFKGSIKPVDG GDPLVVAIKQLSKDGLQGHK
Sbjct: 61  RVFSFTELRQATQDFNRLLKIGQGGFGSVFKGSIKPVDGNGDPLVVAIKQLSKDGLQGHK 120

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAW 180
           QWLAEVQFLGIVEHPNLVKLIGYCAVDG+RGIQRLLVYEYM NRSLEDHLFNKALP LAW
Sbjct: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGSRGIQRLLVYEYMPNRSLEDHLFNKALPPLAW 180

Query: 181 KTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240
           +TRL IVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT
Sbjct: 181 RTRLHIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRT 240

Query: 241 HVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEW 300
           HVSTAVMGTNGYAAPDYI+TGHLTAKSDVWS GVVLYEILTGRRSL+R+R R E KLVEW
Sbjct: 241 HVSTAVMGTNGYAAPDYIETGHLTAKSDVWSLGVVLYEILTGRRSLERNRSRFEHKLVEW 300

Query: 301 VKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQII 360
           VK+FNPDSK+FSLIIDPRLENQYPINAAR++AKLADTCLAK+AKDRPSMAEVVN LK+II
Sbjct: 301 VKHFNPDSKKFSLIIDPRLENQYPINAARKLAKLADTCLAKNAKDRPSMAEVVNSLKEII 360

Query: 361 KDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRFM 420
           K +D+  PY+KSPDS+ DEP+MEREPEKME  AP+SWRRRMSHLQKLGEHVEGASRRRFM
Sbjct: 361 KSEDNSKPYEKSPDSVIDEPDMEREPEKME--APKSWRRRMSHLQKLGEHVEGASRRRFM 420

Query: 421 IMQRAKVP 429
           IMQ AKVP
Sbjct: 421 IMQSAKVP 426

BLAST of Lag0020931 vs. ExPASy TrEMBL
Match: A0A6J1FKY9 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=LOC111446363 PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.3e-217
Identity = 378/429 (88.11%), Postives = 407/429 (94.87%), Query Frame = 0

Query: 1   MKCFFYFRDKTRS-KHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHN 60
           MKCFFYF++K+RS +HQRSAPELK + ES+FSGCS+T ASSCSL S+RSVPELYEEKAHN
Sbjct: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESNFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAE+R+ATHDF+RLLKIGQGGFGSVFKGSIKP+DGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLV+L+GYCAVDG RGIQRLLVYEYMTNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPE GR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS+GVVLYEILTGRRSL+RH PRPEQ LVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQI 360
           WVKY NPDS++FS IIDPRLEN+YPIN AR+VAKLA+TCL K+AKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKDKDDEAPYKKSPDSIDDEPEMEREPEKMEAQAPESWRRRMSHLQKLGEHVEGASRRRF 420
           IK KDD  PY+KSPD+ D++P+MEREPEK +    +SWRRRMSHLQKLGEHVEGASRRRF
Sbjct: 361 IKAKDDNEPYEKSPDNSDNKPDMEREPEKTKVM--DSWRRRMSHLQKLGEHVEGASRRRF 420

Query: 421 MIMQRAKVP 429
           MIMQRAKVP
Sbjct: 421 MIMQRAKVP 427

BLAST of Lag0020931 vs. TAIR 10
Match: AT5G47070.1 (Protein kinase superfamily protein )

HSP 1 Score: 427.6 bits (1098), Expect = 1.2e-119
Identity = 224/404 (55.45%), Postives = 293/404 (72.52%), Query Frame = 0

Query: 1   MKCFFYFR-------DKTRSKHQRSAPELKEQQESDFSGCSRTGASSC----SLASSRSV 60
           M C F F+        K  +K++R   EL +    + +  S T + +     SL S RS+
Sbjct: 1   MNCLFLFKSKKPRKQQKDNNKNKRKGKELLQNSAPELTNRSETSSFNLQTPRSLPSPRSI 60

Query: 61  PELYEEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSI-KPVDGKGDPLVVAI 120
            +LY E+  NLRVFS+ EL +AT+ F R L IG+GGFG V+KG I    D    PLVVAI
Sbjct: 61  KDLYTEREQNLRVFSYEELSKATYVFSRKLVIGEGGFGIVYKGKILSNGDSSDPPLVVAI 120

Query: 121 KQLSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLED 180
           K+L++ GLQGHKQWLAEVQFLG+V HPN+VKLIGYC+ DG  GI+RLLVYEYM+NRSLED
Sbjct: 121 KKLNRQGLQGHKQWLAEVQFLGVVNHPNVVKLIGYCSEDGETGIERLLVYEYMSNRSLED 180

Query: 181 HLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDF 240
           HLF +   TL WK RL+I+LGAA+GL YLH   ++++IYRDFKSSNVLLD+ F PKLSDF
Sbjct: 181 HLFPRRSHTLPWKKRLEIMLGAAEGLTYLH---DLKVIYRDFKSSNVLLDDQFCPKLSDF 240

Query: 241 GLAREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDR 300
           GLAREGP+   THV+TA +GT+GYAAP+Y++TGHL  KSDV+SFGVVLYEI+TGRR+++R
Sbjct: 241 GLAREGPDGDNTHVTTARVGTHGYAAPEYVQTGHLRLKSDVYSFGVVLYEIITGRRTIER 300

Query: 301 HRPRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPS 360
           ++P  E++L++WVK +  DS+RFS+I+DPRL N YP   AR +AKLAD CL K+ K+RP+
Sbjct: 301 NKPVAERRLLDWVKEYPADSQRFSMIVDPRLRNNYPAAGARSLAKLADLCLKKNDKERPT 360

Query: 361 MAEVVNRLKQIIKDKDDEAPYKKSPDSIDDEPEMER----EPEK 389
           M  VV RLK+II++ D E  Y  +  +  +  ++ R    +PEK
Sbjct: 361 MEIVVERLKKIIEESDSE-DYPMATTTTKESSQVRRRQVAKPEK 400

BLAST of Lag0020931 vs. TAIR 10
Match: AT2G39110.1 (Protein kinase superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.3e-110
Identity = 197/369 (53.39%), Postives = 264/369 (71.54%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKAHNL 60
           MKCF++ +DKT+    ++            SG      +S + + + S+  L E  ++NL
Sbjct: 16  MKCFYFSKDKTQDDEAKTRKFGSATMARGGSGSEFNSDTSTATSITSSLHVLSETHSNNL 75

Query: 61  RVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQLSKDGLQGHK 120
           +VF   +L+ AT +F R L IG+GGFG VF+G I+        + +A+KQLS+ GLQGHK
Sbjct: 76  KVFVLDDLKTATKNFSRSLMIGEGGFGGVFRGVIQNPQDSRKKIDIAVKQLSRRGLQGHK 135

Query: 121 QWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPT-LA 180
           +W+ EV  LG+VEHPNLVKLIGYCA D  RGIQRLLVYEY+ NRS++DHL N+ + T L 
Sbjct: 136 EWVTEVNVLGVVEHPNLVKLIGYCAEDDERGIQRLLVYEYVQNRSVQDHLSNRFIVTPLP 195

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGR 240
           W TRL+I    A+GLAYLH+G+E QII+RDFKSSN+LLDEN++ KLSDFGLAR GP  G 
Sbjct: 196 WSTRLKIAQDTARGLAYLHQGMEFQIIFRDFKSSNILLDENWNAKLSDFGLARMGPSDGI 255

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVE 300
           THVSTAV+GT GYAAP+YI+TGHLTAKSDVWS+G+ LYE++TGRR  DR+RPR EQ ++E
Sbjct: 256 THVSTAVVGTIGYAAPEYIQTGHLTAKSDVWSYGIFLYELITGRRPFDRNRPRNEQNILE 315

Query: 301 WVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQI 360
           W++    D K+F +IIDPRLE  Y + +A ++A +A+ CL   AK RP+M++V   L++I
Sbjct: 316 WIRPHLSDIKKFKMIIDPRLEGNYYLKSALKLAAVANRCLMVKAKARPTMSQVSEMLERI 375

Query: 361 IKDKDDEAP 369
           ++   D AP
Sbjct: 376 VETSSDGAP 384

BLAST of Lag0020931 vs. TAIR 10
Match: AT4G17660.1 (Protein kinase superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 5.7e-109
Identity = 209/358 (58.38%), Postives = 262/358 (73.18%), Query Frame = 0

Query: 16  QRSAPELKEQQESDFSGCSRTGASSCSLASSRSVPELYEEKA----HNLRVFSFAELRQA 75
           Q+SAPEL  ++ +     S    +  SL S  S+ +LY ++      NLRVFSF EL  A
Sbjct: 32  QKSAPELTTRKTT----LSFNLPTPRSLPSPTSIKDLYTDREQNQNQNLRVFSFKELSDA 91

Query: 76  THDFDRLLKIGQGGFGSVFKGSI-KPV--DGKGDPLVVAIKQLSKDGLQGHKQWLAEVQF 135
           T +F R LKIG+GGFGSV+K +I  P   D    PL VA+K+L++  LQGHKQWLAEV F
Sbjct: 92  TCEFSRKLKIGEGGFGSVYKATINNPTVGDSHSSPLTVAVKKLNRQSLQGHKQWLAEVHF 151

Query: 136 LGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHLFNKALPTLAWKTRLQIVL 195
           LG+V HPN+V+L+GYC+ D     +RLLVYE M+NRSLEDHLF     TL+WK RL+I+L
Sbjct: 152 LGVVNHPNVVRLLGYCSEDR----ERLLVYELMSNRSLEDHLFTLRTLTLSWKQRLEIML 211

Query: 196 GAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGRTHVSTAVMG 255
           GAAQGLAYLH   E+Q+IYRDFKSSNVLL+E FHPKLSDFGLAREGPE   THV+TA +G
Sbjct: 212 GAAQGLAYLH---EIQVIYRDFKSSNVLLNEEFHPKLSDFGLAREGPEGDNTHVTTARVG 271

Query: 256 TNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHRPRPEQKLVEWVKYFNPDS 315
           T+GYAAP+Y+ TGHL    DV+SFGVVLYEI+TGRR+L+R +P  EQKL+EWVK +  +S
Sbjct: 272 TDGYAAPEYVITGHLKTHCDVYSFGVVLYEIITGRRTLERMKPLAEQKLLEWVKKYPINS 331

Query: 316 KRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMAEVVNRLKQIIKDKDDE 367
           KRF +I+D +L N+YPI   R VAKLAD C+ K  K+RP+MA VV  L  II++ + E
Sbjct: 332 KRFKMIVDSKLCNKYPIAMVRRVAKLADHCVNKIDKERPTMAFVVESLTNIIEESNSE 378

BLAST of Lag0020931 vs. TAIR 10
Match: AT3G09830.1 (Protein kinase superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.0e-105
Identity = 192/371 (51.75%), Postives = 263/371 (70.89%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQR----SAPELKEQQE-----SDFSGCSRTGASS-CSLASSRSVP 60
           MKCF +     R + +     S   +   +E     S+F+    +G S+  S+    S P
Sbjct: 1   MKCFLFSGGDKRGEQKTPISVSLTSIFSDREINRSGSEFNSRDVSGTSTESSMGRKNSYP 60

Query: 61  ELYEEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQ 120
            +   +A NLR FS  +L+ AT +F R + IG+GGFG VF+G+++ ++     + VA+KQ
Sbjct: 61  PV-STRASNLREFSITDLKSATKNFSRSVMIGEGGFGCVFRGTVRNLEDSSVKIEVAVKQ 120

Query: 121 LSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHL 180
           L K GLQGHK+W+ EV FLGIVEH NLVKL+GYCA D  RGIQRLLVYEYM NRS+E HL
Sbjct: 121 LGKRGLQGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHL 180

Query: 181 FNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGL 240
             ++L  L W  RL+I   AA+GL YLHE +E QII+RDFKSSN+LLDE++  KLSDFGL
Sbjct: 181 SPRSLTVLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGL 240

Query: 241 AREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHR 300
           AR GP  G THVST V+GT GYAAP+YI+TG LT+KSDVW +GV LYE++TGRR +DR+R
Sbjct: 241 ARLGPSEGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRRPVDRNR 300

Query: 301 PRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMA 360
           P+ EQKL+EWV+ +  D+++F LI+DPRLE +YPI + +++A +A+ CL +++K RP M+
Sbjct: 301 PKGEQKLLEWVRPYLSDTRKFKLILDPRLEGKYPIKSVQKLAVVANRCLVRNSKARPKMS 360

Query: 361 EVVNRLKQIIK 362
           EV+  + +I++
Sbjct: 361 EVLEMVNKIVE 370

BLAST of Lag0020931 vs. TAIR 10
Match: AT3G09830.2 (Protein kinase superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.0e-105
Identity = 192/371 (51.75%), Postives = 263/371 (70.89%), Query Frame = 0

Query: 1   MKCFFYFRDKTRSKHQR----SAPELKEQQE-----SDFSGCSRTGASS-CSLASSRSVP 60
           MKCF +     R + +     S   +   +E     S+F+    +G S+  S+    S P
Sbjct: 1   MKCFLFSGGDKRGEQKTPISVSLTSIFSDREINRSGSEFNSRDVSGTSTESSMGRKNSYP 60

Query: 61  ELYEEKAHNLRVFSFAELRQATHDFDRLLKIGQGGFGSVFKGSIKPVDGKGDPLVVAIKQ 120
            +   +A NLR FS  +L+ AT +F R + IG+GGFG VF+G+++ ++     + VA+KQ
Sbjct: 61  PV-STRASNLREFSITDLKSATKNFSRSVMIGEGGFGCVFRGTVRNLEDSSVKIEVAVKQ 120

Query: 121 LSKDGLQGHKQWLAEVQFLGIVEHPNLVKLIGYCAVDGARGIQRLLVYEYMTNRSLEDHL 180
           L K GLQGHK+W+ EV FLGIVEH NLVKL+GYCA D  RGIQRLLVYEYM NRS+E HL
Sbjct: 121 LGKRGLQGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHL 180

Query: 181 FNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGL 240
             ++L  L W  RL+I   AA+GL YLHE +E QII+RDFKSSN+LLDE++  KLSDFGL
Sbjct: 181 SPRSLTVLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGL 240

Query: 241 AREGPEIGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSFGVVLYEILTGRRSLDRHR 300
           AR GP  G THVST V+GT GYAAP+YI+TG LT+KSDVW +GV LYE++TGRR +DR+R
Sbjct: 241 ARLGPSEGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRRPVDRNR 300

Query: 301 PRPEQKLVEWVKYFNPDSKRFSLIIDPRLENQYPINAAREVAKLADTCLAKSAKDRPSMA 360
           P+ EQKL+EWV+ +  D+++F LI+DPRLE +YPI + +++A +A+ CL +++K RP M+
Sbjct: 301 PKGEQKLLEWVRPYLSDTRKFKLILDPRLEGKYPIKSVQKLAVVANRCLVRNSKARPKMS 360

Query: 361 EVVNRLKQIIK 362
           EV+  + +I++
Sbjct: 361 EVLEMVNKIVE 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6582509.12.3e-22191.59putative serine/threonine-protein kinase PBL19, partial [Cucurbita argyrosperma ... [more]
XP_022924530.13.9e-22191.36probable serine/threonine-protein kinase PBL19 [Cucurbita moschata] >XP_02292453... [more]
XP_038892560.15.7e-22089.95probable serine/threonine-protein kinase PBL19 [Benincasa hispida][more]
XP_023527511.17.4e-22090.89probable serine/threonine-protein kinase PBL19 [Cucurbita pepo subsp. pepo] >XP_... [more]
XP_022980299.11.1e-21890.65probable serine/threonine-protein kinase PBL19 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LTC01.7e-11855.45Probable serine/threonine-protein kinase PBL19 OS=Arabidopsis thaliana OX=3702 G... [more]
F4JPX38.0e-10858.38Probable serine/threonine-protein kinase PBL20 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9SF861.4e-10451.75Serine/threonine-protein kinase PCRK1 OS=Arabidopsis thaliana OX=3702 GN=PCRK1 P... [more]
Q9LZF83.2e-10151.36Serine/threonine-protein kinase PCRK2 OS=Arabidopsis thaliana OX=3702 GN=PCRK2 P... [more]
Q9LFP71.0e-9153.82Serine/threonine-protein kinase PBL34 OS=Arabidopsis thaliana OX=3702 GN=PBL34 P... [more]
Match NameE-valueIdentityDescription
A0A6J1EFA41.9e-22191.36probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IYW55.2e-21990.65probable serine/threonine-protein kinase PBL19 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1IB575.7e-21888.34probable serine/threonine-protein kinase PBL19 isoform X2 OS=Cucurbita maxima OX... [more]
A0A0A0L4J85.7e-21889.49Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128... [more]
A0A6J1FKY96.3e-21788.11probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT5G47070.11.2e-11955.45Protein kinase superfamily protein [more]
AT2G39110.12.3e-11053.39Protein kinase superfamily protein [more]
AT4G17660.15.7e-10958.38Protein kinase superfamily protein [more]
AT3G09830.11.0e-10551.75Protein kinase superfamily protein [more]
AT3G09830.21.0e-10551.75Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 161..379
e-value: 3.5E-63
score: 214.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 47..160
e-value: 5.1E-27
score: 96.0
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 75..283
e-value: 1.8E-26
score: 90.6
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 80..285
e-value: 5.5E-12
score: 43.4
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 67..283
e-value: 1.9E-20
score: 70.9
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 69..278
e-value: 1.6E-10
score: 37.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..378
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..397
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..41
NoneNo IPR availablePANTHERPTHR45621:SF190OS07G0495150 PROTEINcoord: 22..412
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 22..412
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 81..359
e-value: 7.64492E-90
score: 271.454
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 80..356
e-value: 6.0E-47
score: 160.1
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 205..217
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 81..109
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 75..360
score: 39.763901
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 58..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020931.1Lag0020931.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity