Lag0020922 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020922
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	PMEI domain-containing protein
Location	chr7: 3185637 .. 3186188 (-)
RNA-Seq Expression	Lag0020922
Synteny	Lag0020922

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTATGCTAATACGCACCGTTTCATTGCGCTCGCATTTTCCTTCTTCGTCGCCTTCAATTTCGCAGAAGCCAACGACGTCGTTTCACGAACCTGCGAGATCTCCACCGCGAGCGATCCGAACGTCCACCTCGACTTCTGCCTGCGTTCTCTCGCCGCCGCACCGGGGAGTGACACGGCGGACCTGTTCGGACTCGGCGCGATCTCGATCGAGTTGATCGGAAAGAACGCGACCAGTACGCGGCGGTACATCAAGCGCTTGTTGAAGAAGAAGAATCCGTCGCCGGACTCGTTCGTGAGGCCGCGGCTGTGCGATTGCCTAGAGCTGTACTCCGACGCCGTCGAGACGGTGCGCGAGGCGGCGGTGGAGTACGCTGCGAAGCGCCACGACGAGGCGAACGTGAAGTTGAGTTCGGTCATGGATGCGGTTACGACGTGCGAGGACGGATTCAAGGAAATGGACGGCGGAGTTTCGCCGTTGACGAAGAGGAACGGCGACGCGTTTGAGTTAACCGCCATCGCGCTGTGTATTCTCAACATGCGTCCTTGA

mRNA sequence

ATGATTTATGCTAATACGCACCGTTTCATTGCGCTCGCATTTTCCTTCTTCGTCGCCTTCAATTTCGCAGAAGCCAACGACGTCGTTTCACGAACCTGCGAGATCTCCACCGCGAGCGATCCGAACGTCCACCTCGACTTCTGCCTGCGTTCTCTCGCCGCCGCACCGGGGAGTGACACGGCGGACCTGTTCGGACTCGGCGCGATCTCGATCGAGTTGATCGGAAAGAACGCGACCAGTACGCGGCGGTACATCAAGCGCTTGTTGAAGAAGAAGAATCCGTCGCCGGACTCGTTCGTGAGGCCGCGGCTGTGCGATTGCCTAGAGCTGTACTCCGACGCCGTCGAGACGGTGCGCGAGGCGGCGGTGGAGTACGCTGCGAAGCGCCACGACGAGGCGAACGTGAAGTTGAGTTCGGTCATGGATGCGGTTACGACGTGCGAGGACGGATTCAAGGAAATGGACGGCGGAGTTTCGCCGTTGACGAAGAGGAACGGCGACGCGTTTGAGTTAACCGCCATCGCGCTGTGTATTCTCAACATGCGTCCTTGA

Coding sequence (CDS)

ATGATTTATGCTAATACGCACCGTTTCATTGCGCTCGCATTTTCCTTCTTCGTCGCCTTCAATTTCGCAGAAGCCAACGACGTCGTTTCACGAACCTGCGAGATCTCCACCGCGAGCGATCCGAACGTCCACCTCGACTTCTGCCTGCGTTCTCTCGCCGCCGCACCGGGGAGTGACACGGCGGACCTGTTCGGACTCGGCGCGATCTCGATCGAGTTGATCGGAAAGAACGCGACCAGTACGCGGCGGTACATCAAGCGCTTGTTGAAGAAGAAGAATCCGTCGCCGGACTCGTTCGTGAGGCCGCGGCTGTGCGATTGCCTAGAGCTGTACTCCGACGCCGTCGAGACGGTGCGCGAGGCGGCGGTGGAGTACGCTGCGAAGCGCCACGACGAGGCGAACGTGAAGTTGAGTTCGGTCATGGATGCGGTTACGACGTGCGAGGACGGATTCAAGGAAATGGACGGCGGAGTTTCGCCGTTGACGAAGAGGAACGGCGACGCGTTTGAGTTAACCGCCATCGCGCTGTGTATTCTCAACATGCGTCCTTGA

Protein sequence

MIYANTHRFIALAFSFFVAFNFAEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTADLFGLGAISIELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAVETVREAAVEYAAKRHDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNMRP

Homology

[Close]

BLAST of Lag0020922 vs. NCBI nr
Match: XP_023528439.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 279.6 bits (714), Expect = 1.9e-71
Identity = 148/184 (80.43%), Postives = 158/184 (85.87%), Query Frame = 0


Query: 6   THRFIALAFSFFVA--FNFAEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTADL 65
           THRFIALA S FVA   N   ANDVV+RTCEIS A DPNVHLDFCLRSLAAAP SD ADL
Sbjct: 6   THRFIALASSLFVALYLNSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDMADL 65

Query: 66  FGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAVETVR 125
           + LGAISIELIG+NATSTRRYI+RLL    KKKNPS DS+VRPRL DC ELYSD   TVR
Sbjct: 66  YELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDVKATVR 125

Query: 126 EAAVEYAAKRHDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCIL 184
           +AA +YA+KR++E NVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRN DAFELTAIALCIL
Sbjct: 126 DAAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCIL 185

[Close]

BLAST of Lag0020922 vs. NCBI nr
Match: XP_038905481.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 278.9 bits (712), Expect = 3.2e-71
Identity = 144/186 (77.42%), Postives = 160/186 (86.02%), Query Frame = 0


Query: 1   MIYANTHRFIALAFSFFVAFNF--AEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGS 60
           M +  THRFIALA+SF +AF+F    +ND+V RTCEIS ASDPNV LDFCLRSLAA P S
Sbjct: 1   MSFTYTHRFIALAYSFLLAFSFQSTASNDIVLRTCEISAASDPNVRLDFCLRSLAADPAS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLKKKNP-SPDSFVRPRLCDCLELYSDAVET 120
           DTADL+ LGAISIELIG+NATSTRRYI RLLKKK   SPDS+VRPRL DC ELYSDAVET
Sbjct: 61  DTADLYELGAISIELIGRNATSTRRYIDRLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120

Query: 121 VREAAVEYAAKRHDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALC 180
           V +++ EYA KR++E NVKLSSVMDAVTTCEDGFKEM+GG+SPLTKRNGD FELTAIALC
Sbjct: 121 VGDSSAEYARKRYEEVNVKLSSVMDAVTTCEDGFKEMEGGISPLTKRNGDVFELTAIALC 180

Query: 181 ILNMRP 184
           ILNM P
Sbjct: 181 ILNMHP 186

[Close]

BLAST of Lag0020922 vs. NCBI nr
Match: KAG6582515.1 (hypothetical protein SDJN03_22517, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018899.1 hypothetical protein SDJN02_20772, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.9 bits (712), Expect = 3.2e-71
Identity = 147/184 (79.89%), Postives = 158/184 (85.87%), Query Frame = 0


Query: 6   THRFIALAFSFFVA--FNFAEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTADL 65
           THRFIALA S FVA   N   ANDVV+RTCEIS A DPNVHLDFCLRSLAAAP SDTADL
Sbjct: 6   THRFIALASSLFVALYLNSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDTADL 65

Query: 66  FGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAVETVR 125
           + LGAISIELIG+NATSTRRYI+RLL    KKKNPSPDS++RP L DC ELYSD   TVR
Sbjct: 66  YELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSPDSYLRPPLNDCRELYSDVRVTVR 125

Query: 126 EAAVEYAAKRHDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCIL 184
           +AA +YA+KR++E NVKLSSVMDA TTCEDGFKEMDGGVSPLTKRN DAFELTAIALCIL
Sbjct: 126 DAAADYASKRYEEVNVKLSSVMDAATTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCIL 185

[Close]

BLAST of Lag0020922 vs. NCBI nr
Match: XP_022924291.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 278.5 bits (711), Expect = 4.2e-71
Identity = 146/184 (79.35%), Postives = 158/184 (85.87%), Query Frame = 0


Query: 6   THRFIALAFSFFVAFNFAE--ANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTADL 65
           THRFIALA S FVA       ANDVV+RTCEIS A DPNVHLDFCLRSLAAAP SDTADL
Sbjct: 6   THRFIALASSLFVALYLTSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDTADL 65

Query: 66  FGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAVETVR 125
           + LGAISIELIG+NATSTRRYI+RLL    KKKNPSPDS++RP L DC ELYSD   TVR
Sbjct: 66  YELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDVRVTVR 125

Query: 126 EAAVEYAAKRHDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCIL 184
           +AA +YA+KR++E NVKLSS+MDAVTTCEDGFKEMDGGVSPLTKRN DAFELTAIALCIL
Sbjct: 126 DAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCIL 185

[Close]

BLAST of Lag0020922 vs. NCBI nr
Match: XP_008438273.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 270.0 bits (689), Expect = 1.5e-68
Identity = 142/187 (75.94%), Postives = 155/187 (82.89%), Query Frame = 0


Query: 1   MIYANTHRFIALAFSFFVAFNF--AEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGS 60
           M +  THR+IALAFSF V  NF    AND+VSRTCE S A DPNV LDFCLRSLAAAPGS
Sbjct: 1   MSFTYTHRYIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLK--KKNPSPDSFVRPRLCDCLELYSDAVE 120
           DTADL+ LGAISI LIG+NATST+RYI+RLLK  KK  S DS++RPRL DC ELYSDAVE
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVREAAVEYAAKRHDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIAL 180
           TV EAA EY  KR+DE NVKLSSVMDAVTTCEDGFKEM+  VSPLTKRNGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 184
           CIL++RP
Sbjct: 181 CILDLRP 187

[Close]

BLAST of Lag0020922 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.2e-29
Identity = 71/168 (42.26%), Postives = 102/168 (60.71%), Query Frame = 0


Query: 14  FSFFVAFNFAEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTADLFGLGAISIEL 73
           F+  +      + D+V  TC+      PNV+ DFC++SL A P S TADL GLG IS  L
Sbjct: 12  FNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANL 71

Query: 74  IGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAVETVREAAVEYAAKRHDEA 133
             ++ +  + +I R+LK K    D  ++  L DC+ LY+DA  +V+EA  ++ +K +  A
Sbjct: 72  AIQHGSKIQTFIGRILKSK---VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASA 131

Query: 134 NVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
           NVK+S+ +D   TCEDGFKE  G VSP+TK N D  +LTAI+L I  +
Sbjct: 132 NVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Lag0020922 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.0e-28
Identity = 71/170 (41.76%), Postives = 102/170 (60.00%), Query Frame = 0


Query: 12  LAFSFFVAFNFAEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTADLFGLGAISI 71
           +  SF +   F  + D+V  TC+      PNV+ DFC++SL A P S +ADL GLG IS 
Sbjct: 1   MKLSFSLCIFFLISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISA 60

Query: 72  ELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAVETVREAAVEYAAKRHD 131
            L  +  +  + +I R+LK K    D  ++  L DC+ LY+DA  +V+EA  ++ +K + 
Sbjct: 61  NLAIQQGSKIQTFIGRILKSK---VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYA 120

Query: 132 EANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
            ANVK+S+ +D   TCEDGFKE  G  SP+TK N D  +LTAI+L I  +
Sbjct: 121 SANVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of Lag0020922 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.3e-10
Identity = 51/169 (30.18%), Postives = 77/169 (45.56%), Query Frame = 0


Query: 14  FSFFVAFNFAEANDVVSRTCEISTASDPNVHLDFCLRSLAAAPGSDTA-DLFGLGAISIE 73
           FS F+   FA A  ++  +C+ + A DP +  DFC+ SL   P S  A  L  L   S +
Sbjct: 10  FSLFLN-GFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTK 69

Query: 74  LIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAVETVREAAVEYAAKRHDE 133
                 T+ +  + + LK +       +   L  CL  Y+DA + +  A     ++ +  
Sbjct: 70  TAAAKITNLKGIVAQDLKDQRYQD---IVEDLKLCLGFYNDANDDLTTALANIKSRDYQG 129

Query: 134 ANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
           AN+ LS+ +D    CED FKE     SP+T  N   F+   I L   NM
Sbjct: 130 ANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

The following BLAST results are available for this feature:

BLAST of Lag0020922 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528439.1	1.9e-71	80.43	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
XP_038905481.1	3.2e-71	77.42	putative invertase inhibitor [Benincasa hispida]	[more]
KAG6582515.1	3.2e-71	79.89	hypothetical protein SDJN03_22517, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022924291.1	4.2e-71	79.35	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_008438273.1	1.5e-68	75.94	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]

BLAST of Lag0020922 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	9.2e-29	42.26	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	2.0e-28	41.76	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	3.3e-10	30.18	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 24..178 e-value: 9.1E-25 score: 98.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..178 e-value: 3.8E-26 score: 92.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..181 e-value: 1.0E-33 score: 114.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 24..181 e-value: 1.3E-34 score: 121.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 26..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 17..180
None	No IPR available	PANTHER	PTHR31080:SF226	PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATED	coord: 17..180
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 33..181 e-value: 5.33655E-47 score: 149.052

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020922.1	Lag0020922.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity